Rh4BG336000

Plays an essential role in chain termination during de novo fatty acid synthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0014724
Physical Location & Seq
Forward (+)
49934073 .. 49935537
1465 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG336000.1

Sequence Viewer

Length: 915 bp
ATGGCCACCTTCTCAACTCTAGCCTACTTCCCCATTACAATTAGGTTCTCCTGGAATAGGGCCAGTTATGATCCGAACAAGAAGAGCCTGAACAACATCAAGATTAATGGTACTTCAACAAAGTCCACAACCCAGCTTGACATGGCAAGCGCTTTAACCTCAGTTGCTTACATTAGTGAACAGCAAGTTCGACAGAATATTCCGACAACCAAGCAGTTTGTTGATCATTATCGCCAAGGGATGATCATCGAAGGTGGTGTAGGGTACAGACAGAGGGTGGTTATTAGGTCTTATGAAGTCGGTCCTGATAAAACTGTAACATTGGAGAGCATCCTTAATCTTCTGCAGGAAACAGCATTGAATCATGTGTGGATGTCAGGACTCCTCAGTGATGGGTTTGGAGCAACTCATGGAATGATGAAGCATGATCTCATATGGGTTGTGTCAAGAATGCAAGTTGAAGTTGATCACTATCCAATCTGGGGAGAAGTATTGGAAATTGACACTTGGGTTGGGGCATCAGGGAAGAATGGTATGCGTCGAGACTGGTTAATCCGAAGTGAAGCCACAGGTCATGTTTTTGCTAGTGCAACCAGCACCTGGGTGATGATGAACCGAACAACAAGACGCCTCTCAAAAATGCCAGAAGAAGTGAGAGCTGAGATTGCACCTTGGTTTATAGAGAAGCAAGCAATCACAGAAGATGTCCTAGAGAAAATTGTCAAATTGGACAACAAAGCCAAGTACATGAACAACGACTTGAAGGTAAAAAGACTCCTACACCACAAGTCAGGGTTAGAAAGAATTTTAGCATATGCTGAATATGTAATTTTCTTACTATGTGTAGCCCAAGAGAAGCGACTTGGACATGAACCAGCATGTCAACAATGTCAAGTATGTGAGGTGGATGCTTGA

Protein Analysis

304

Amino Acids

34.69

Weight (kDa)

8.56

Isoelectric Point (pI)

44.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acyl-ACP_TE PF01643 88 - 222 3.4e-34 Acyl-ACP thioesterase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014724)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g26420
malus_domestica MD13G1032900.v1.1 MD16G1034900.v1.1
prunus_persica Prupe.1G319800_v2.0.a1
pyrus_communis pycom13g02840 pycom16g02970
rosa_chinensis RchiOBHm_Chr4g0434051
rosa_laevigata RLG00000006705
rosa_multiflora Rmu_sc0000352.1_g000027 Rmu_sc0000352.1_g000028
rosa_roxburghii Rroxscaffold_5G00375460
rosa_rugosa Rorug04G0273000
rosa_samantha Rh4AG328700 Rh4BG336000 Rh4CG351500 Rh4DG332400
rosa_wichuraiana Rw4G028500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 600
AclWI GGATC 1 cut(s) 65
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 804
AcyI GRCGYC 1 cut(s) 628
AfaI GTAC 3 cut(s) 112, 266, 746
AfeI AGCGCT 1 cut(s) 151
AfiI CCNNNNNNNGG 3 cut(s) 57, 482, 600
AgsI TTSAA 4 cut(s) 117, 361, 461, 763
AjnI CCWGG 2 cut(s) 50, 599
AleI CACNNNNGTG 1 cut(s) 602
AluBI AGCT 2 cut(s) 136, 659
AluI AGCT 2 cut(s) 136, 659
Alw26I GTCTC 1 cut(s) 537
AlwI GGATC 1 cut(s) 65
AlwNI CAGNNNCTG 1 cut(s) 600
Aor51HI AGCGCT 1 cut(s) 151
AoxI GGCC 2 cut(s) 3, 60
ApoI RAATTY 1 cut(s) 804
AseI ATTAAT 1 cut(s) 105
AspLEI GCGC 1 cut(s) 152
AspS9I GGNCC 2 cut(s) 60, 302
AsuHPI GGTGA 1 cut(s) 616
AvaII GGWCC 1 cut(s) 302
BalI TGGCCA 1 cut(s) 5
BccI CCATC 1 cut(s) 386
BciT130I CCWGG 2 cut(s) 52, 601
BclI TGATCA 3 cut(s) 223, 243, 466
BcoDI GTCTC 1 cut(s) 537
BfaI CTAG 3 cut(s) 20, 585, 710
BfmI CTRYAG 1 cut(s) 344
BfoI RGCGCY 1 cut(s) 153
Bme1390I CCNGG 2 cut(s) 52, 601
Bme18I GGWCC 1 cut(s) 302
BmgT120I GGNCC 2 cut(s) 60, 302
BmrFI CCNGG 2 cut(s) 52, 601
BmsI GCATC 3 cut(s) 339, 527, 898
BsaBI GATNNNNATC 3 cut(s) 228, 245, 471
BsaHI GRCGYC 1 cut(s) 628
BsaJI CCNNGG 3 cut(s) 235, 600, 671
Bsc4I CCNNNNNNNGG 3 cut(s) 57, 482, 600
Bse1I ACTGG 2 cut(s) 63, 551
Bse8I GATNNNNATC 3 cut(s) 228, 245, 471
BseBI CCWGG 2 cut(s) 52, 601
BseDI CCNNGG 3 cut(s) 235, 600, 671
BseGI GGATG 4 cut(s) 246, 330, 378, 913
BseJI GATNNNNATC 3 cut(s) 228, 245, 471
BseLI CCNNNNNNNGG 3 cut(s) 57, 482, 600
BseMII CTCAG 3 cut(s) 174, 400, 651
BseNI ACTGG 2 cut(s) 63, 551
BseRI GAGGAG 1 cut(s) 374
BseYI CCCAGC 1 cut(s) 132
BshFI GGCC 2 cut(s) 5, 62
BslI CCNNNNNNNGG 3 cut(s) 57, 482, 600
BsmAI GTCTC 1 cut(s) 537
BsmI GAATGC 1 cut(s) 456
BsnI GGCC 2 cut(s) 5, 62
Bsp143I GATC 5 cut(s) 70, 223, 243, 427, 466
BspANI GGCC 2 cut(s) 5, 62
BspCNI CTCAG 3 cut(s) 173, 399, 652
BspMAI CTGCAG 1 cut(s) 348
BspPI GGATC 1 cut(s) 65
BspQI GCTCTTC 1 cut(s) 77
BsrI ACTGG 2 cut(s) 63, 551
BssECI CCNNGG 3 cut(s) 235, 600, 671
BssMI GATC 5 cut(s) 70, 223, 243, 427, 466
BssNI GRCGYC 1 cut(s) 628
BssT1I CCWWGG 2 cut(s) 235, 671
Bst2UI CCWGG 2 cut(s) 52, 601
Bst4CI ACNGT 1 cut(s) 316
Bst6I CTCTTC 1 cut(s) 77
BstACI GRCGYC 1 cut(s) 628
BstC8I GCNNGC 2 cut(s) 148, 690
BstDEI CTNAG 3 cut(s) 160, 386, 660
BstENI CCTNNNNNAGG 1 cut(s) 55
BstF5I GGATG 4 cut(s) 246, 330, 378, 913
BstH2I RGCGCY 1 cut(s) 153
BstHHI GCGC 1 cut(s) 152
BstKTI GATC 5 cut(s) 73, 226, 246, 430, 469
BstMAI GTCTC 1 cut(s) 537
BstMBI GATC 5 cut(s) 70, 223, 243, 427, 466
BstMWI GCNNNNNNNGC 1 cut(s) 665
BstNI CCWGG 2 cut(s) 52, 601
BstNSI RCATGY 1 cut(s) 882
BstSCI CCNGG 2 cut(s) 50, 599
BstSFI CTRYAG 1 cut(s) 344
BsuRI GGCC 2 cut(s) 5, 62
BtsCI GGATG 4 cut(s) 246, 330, 378, 913
BtsIMutI CAGTG 1 cut(s) 394
Cac8I GCNNGC 2 cut(s) 148, 690
CaiI CAGNNNCTG 1 cut(s) 600
CfoI GCGC 1 cut(s) 152
Cfr13I GGNCC 2 cut(s) 60, 302
CseI GACGC 2 cut(s) 527, 636
Csp6I GTAC 3 cut(s) 111, 265, 745
CviAII CATG 8 cut(s) 142, 365, 410, 425, 575, 748, 869, 879
CviJI RGCY 9 cut(s) 5, 23, 62, 87, 136, 566, 659, 740, 848
CviKI_1 RGCY 9 cut(s) 5, 23, 62, 87, 136, 566, 659, 740, 848
CviQI GTAC 3 cut(s) 111, 265, 745
DdeI CTNAG 3 cut(s) 160, 386, 660
DpnI GATC 5 cut(s) 72, 225, 245, 429, 468
DpnII GATC 5 cut(s) 70, 223, 243, 427, 466
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 77
EarI CTCTTC 1 cut(s) 77
Eco130I CCWWGG 2 cut(s) 235, 671
Eco47I GGWCC 1 cut(s) 302
Eco47III AGCGCT 1 cut(s) 151
EcoNI CCTNNNNNAGG 1 cut(s) 55
EcoRII CCWGG 2 cut(s) 50, 599
EcoT14I CCWWGG 2 cut(s) 235, 671
ErhI CCWWGG 2 cut(s) 235, 671
FaeI CATG 8 cut(s) 145, 368, 413, 428, 578, 751, 872, 882
FatI CATG 8 cut(s) 141, 364, 409, 424, 574, 747, 868, 878
FauNDI CATATG 2 cut(s) 434, 814
FbaI TGATCA 3 cut(s) 223, 243, 466
FokI GGATG 3 cut(s) 253, 317, 385
FspBI CTAG 3 cut(s) 20, 585, 710
GlaI GCGC 1 cut(s) 151
GsaI CCCAGC 1 cut(s) 136
HaeII RGCGCY 1 cut(s) 153
HaeIII GGCC 2 cut(s) 5, 62
HgaI GACGC 2 cut(s) 527, 636
HhaI GCGC 1 cut(s) 152
Hin1I GRCGYC 1 cut(s) 628
Hin1II CATG 8 cut(s) 145, 368, 413, 428, 578, 751, 872, 882
Hin6I GCGC 1 cut(s) 150
HinP1I GCGC 1 cut(s) 150
HincII GTYRAC 1 cut(s) 884
HindII GTYRAC 1 cut(s) 884
HinfI GANTC 3 cut(s) 361, 381, 774
HphI GGTGA 1 cut(s) 616
Hpy166II GTNNAC 3 cut(s) 126, 179, 884
Hpy188I TCNGA 3 cut(s) 75, 204, 557
Hpy188III TCNNGA 5 cut(s) 100, 305, 378, 447, 542
Hpy8I GTNNAC 3 cut(s) 126, 179, 884
Hpy99I CGWCG 1 cut(s) 543
HpyAV CCTTC 3 cut(s) 19, 245, 757
HpyCH4III ACNGT 1 cut(s) 316
HpyCH4V TGCA 4 cut(s) 346, 454, 590, 668
HpyF10VI GCNNNNNNNGC 1 cut(s) 665
HpyF3I CTNAG 3 cut(s) 160, 386, 660
Hsp92I GRCGYC 1 cut(s) 628
Hsp92II CATG 8 cut(s) 145, 368, 413, 428, 578, 751, 872, 882
HspAI GCGC 1 cut(s) 150
Ksp22I TGATCA 3 cut(s) 223, 243, 466
Kzo9I GATC 5 cut(s) 70, 223, 243, 427, 466
LguI GCTCTTC 1 cut(s) 77
LmnI GCTCC 1 cut(s) 401
LweI GCATC 3 cut(s) 339, 527, 898
MaeI CTAG 3 cut(s) 20, 585, 710
MaeIII GTNAC 1 cut(s) 316
MalI GATC 5 cut(s) 72, 225, 245, 429, 468
MboI GATC 5 cut(s) 70, 223, 243, 427, 466
MboII GAAGA 5 cut(s) 94, 332, 538, 659, 713
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 39, 498, 717, 725, 804, 828
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 375, 768
MmeI TCCRAC 1 cut(s) 227
MnlI CCTC 5 cut(s) 169, 267, 395, 641, 895
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 105, 155, 336, 551
MslI CAYNNNNRTG 1 cut(s) 602
Msp20I TGGCCA 1 cut(s) 5
MspR9I CCNGG 2 cut(s) 52, 601
Mva1269I GAATGC 1 cut(s) 456
MvaI CCWGG 2 cut(s) 52, 601
MwoI GCNNNNNNNGC 1 cut(s) 665
NdeI CATATG 2 cut(s) 434, 814
NdeII GATC 5 cut(s) 70, 223, 243, 427, 466
NlaIII CATG 8 cut(s) 145, 368, 413, 428, 578, 751, 872, 882
NspI RCATGY 1 cut(s) 882
OliI CACNNNNGTG 1 cut(s) 602
PciSI GCTCTTC 1 cut(s) 77
PctI GAATGC 1 cut(s) 456
PfeI GAWTC 1 cut(s) 361
PflMI CCANNNNNTGG 1 cut(s) 600
PfoI TCCNGGA 1 cut(s) 50
PleI GAGTC 2 cut(s) 375, 768
PpsI GAGTC 2 cut(s) 375, 768
PshBI ATTAAT 1 cut(s) 105
Psp6I CCWGG 2 cut(s) 50, 599
PspFI CCCAGC 1 cut(s) 132
PspGI CCWGG 2 cut(s) 50, 599
PspPI GGNCC 2 cut(s) 60, 302
PstI CTGCAG 1 cut(s) 348
PstNI CAGNNNCTG 1 cut(s) 600
RsaI GTAC 3 cut(s) 112, 266, 746
RsaNI GTAC 3 cut(s) 111, 265, 745
RseI CAYNNNNRTG 1 cut(s) 602
SapI GCTCTTC 1 cut(s) 77
SaqAI TTAA 4 cut(s) 105, 155, 336, 551
Sau3AI GATC 5 cut(s) 70, 223, 243, 427, 466
Sau96I GGNCC 2 cut(s) 60, 302
SchI GAGTC 2 cut(s) 375, 768
ScrFI CCNGG 2 cut(s) 52, 601
SfaNI GCATC 3 cut(s) 339, 527, 898
SfcI CTRYAG 1 cut(s) 344
SinI GGWCC 1 cut(s) 302
SmiMI CAYNNNNRTG 1 cut(s) 602
Sse9I AATT 6 cut(s) 39, 498, 717, 725, 804, 828
SspI AATATT 1 cut(s) 199
SspMI CTAG 3 cut(s) 20, 585, 710
StyD4I CCNGG 2 cut(s) 50, 599
StyI CCWWGG 2 cut(s) 235, 671
TaaI ACNGT 1 cut(s) 316
TaqI TCGA 3 cut(s) 190, 249, 541
TaqII GACCGA 1 cut(s) 290
TasI AATT 6 cut(s) 39, 498, 717, 725, 804, 828
TatI WGTACW 1 cut(s) 744
TfiI GAWTC 1 cut(s) 361
Tru1I TTAA 4 cut(s) 105, 155, 336, 551
Tru9I TTAA 4 cut(s) 105, 155, 336, 551
TscAI CASTG 1 cut(s) 394
TspDTI ATGAA 5 cut(s) 309, 434, 626, 764, 885
TspRI CASTG 1 cut(s) 394
Van91I CCANNNNNTGG 1 cut(s) 600
VpaK11BI GGWCC 1 cut(s) 302
VspI ATTAAT 1 cut(s) 105
XagI CCTNNNNNAGG 1 cut(s) 55
XapI RAATTY 1 cut(s) 804
XceI RCATGY 1 cut(s) 882
XspI CTAG 3 cut(s) 20, 585, 710
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.