Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0015229

Physical Location & Seq

Genomic Coordinates Forward (+)

2553901 .. 2554413

Gene Structure 513 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1035500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 513 bp

ATGGGGTTACAAACCCAGCTGAACGACGTCTCATCCGACTCCATCCCACTCCTACTCATCGCACTCATCGCCGGTTCTATCTACCGCCTCCGCTCCTCCCTCCTCACCCTCCTTCATTTCCTGGGTCTCAGCCCCCATCCCGGTCCAGGCACAGACTACGACGAAGGCGATATTGCGGGCCCGACCGTGGGCTCGGGACTCGCGGGTCTCGTTAACCTGTCCGACCAGCTCGCCATCAACCGCCAGTTATCCTATCCATACGACCGTGACGACGCCGTTGCCACCGCACGGGATTGCGATTGCGTGGTGTGCTTGTGCACCTTGAGGGATGGGGAGCTGGTCCGCATGCTCCAGTGCCGCCACGTGTTCCACAAGCACTGCTTCGACTCCTGGCTCAACAACCTCAACTTCAATTGCCCTCTCTGCCGCTCTCCCGTCCTACACCGCGACAGCGGCGCGCTCACGCGCTGCCGACTCTCCCGCGACCTCCTCCACTGGCTTTCCATCCGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009628 GO:0009651 GO:0009787 GO:0009789 GO:0009966 GO:0009967 GO:0009987 GO:0010498 GO:0010646 GO:0010647 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0023051 GO:0023056 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0047484 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051603 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0080134 GO:0140096 GO:1901419 GO:1901421 GO:1901564 GO:1901565 GO:1901575 GO:1905957 GO:1905959

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

171

Amino Acids

18.83

Weight (kDa)

6.35

Isoelectric Point (pI)

42.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	100 - 143	1.6e-10	Ring finger domain
zf-RING_11	PF17123	100 - 127	7.3e-08	RING-like zinc finger
zf-C3HC4_2	PF13923	101 - 142	1.6e-07	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	101 - 142	5.4e-06	Zinc finger, C3HC4 type (RING finger)
zf-rbx1	PF12678	114 - 143	4.5e-07	RING-H2 zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015229)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G15100
fragaria_vesca	FvH4_4g26470
malus_domestica	MD13G1033600.v1.1 MD16G1035500.v1.1
prunus_persica	Prupe.1G319200_v2.0.a1
pyrus_communis	pycom13g02900 pycom16g03040
rosa_chinensis	RchiOBHm_Chr4g0434111
rosa_laevigata	RLG00000006699
rosa_multiflora	Rmu_sc0000352.1_g000035
rosa_roxburghii	Rroxscaffold_5G00375510
rosa_rugosa	Rorug04G0274200
rosa_samantha	Rh4BG336800 Rh4CG352300 Rh4DG332900
rosa_wichuraiana	Rw4G028550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	204
AatII	GACGTC	1 cut(s)	30
AccBSI	CCGCTC	2 cut(s)	93, 429
AccII	CGCG	5 cut(s)	203, 447, 458, 466, 483
AcvI	CACGTG	1 cut(s)	364
AcyI	GRCGYC	2 cut(s)	27, 273
AfiI	CCNNNNNNNGG	5 cut(s)	140, 146, 187, 188, 288
AflIII	ACRYGT	1 cut(s)	363
AgsI	TTSAA	1 cut(s)	412
AjnI	CCWGG	3 cut(s)	120, 145, 389
AluBI	AGCT	3 cut(s)	19, 229, 337
AluI	AGCT	3 cut(s)	19, 229, 337
Alw21I	GWGCWC	1 cut(s)	320
Alw26I	GTCTC	3 cut(s)	34, 131, 212
Alw44I	GTGCAC	1 cut(s)	316
Ama87I	CYCGRG	1 cut(s)	193
AoxI	GGCC	1 cut(s)	178
ApaI	GGGCCC	1 cut(s)	182
ApaLI	GTGCAC	1 cut(s)	316
ApeKI	GCWGC	1 cut(s)	468
AspLEI	GCGC	3 cut(s)	458, 460, 468
AspS9I	GGNCC	4 cut(s)	143, 178, 179, 340
AsuC2I	CCSGG	1 cut(s)	141
AsuHPI	GGTGA	1 cut(s)	97
AvaI	CYCGRG	1 cut(s)	193
AvaII	GGWCC	2 cut(s)	143, 340
BaeGI	GKGCMC	2 cut(s)	182, 320
BanII	GRGCYC	2 cut(s)	182, 194
BbrPI	CACGTG	1 cut(s)	364
Bbv12I	GWGCWC	1 cut(s)	320
BbvI	GCAGC	1 cut(s)	455
BccI	CCATC	5 cut(s)	50, 144, 242, 323, 512
BceAI	ACGGC	1 cut(s)	260
BcgI	CGANNNNNNTGC	2 cut(s)	260, 294
BciT130I	CCWGG	3 cut(s)	122, 147, 391
BcnI	CCSGG	1 cut(s)	141
BcoDI	GTCTC	3 cut(s)	34, 131, 212
BisI	GCNGC	4 cut(s)	358, 427, 454, 469
BlsI	GCNGC	4 cut(s)	359, 428, 455, 470
Bme1390I	CCNGG	4 cut(s)	122, 141, 147, 391
Bme18I	GGWCC	2 cut(s)	143, 340
BmeT110I	CYCGRG	1 cut(s)	193
BmgT120I	GGNCC	4 cut(s)	143, 178, 179, 340
BmiI	GGNNCC	1 cut(s)	180
BmrFI	CCNGG	4 cut(s)	122, 141, 147, 391
BpmI	CTGGAG	1 cut(s)	335
BpuEI	CTTGAG	1 cut(s)	343
BpuMI	CCSGG	1 cut(s)	141
BsaAI	YACGTR	1 cut(s)	364
BsaHI	GRCGYC	2 cut(s)	27, 273
BsaI	GGTCTC	2 cut(s)	131, 212
BsaJI	CCNNGG	2 cut(s)	121, 186
Bsc4I	CCNNNNNNNGG	5 cut(s)	140, 146, 187, 188, 288
Bse118I	RCCGGY	1 cut(s)	71
Bse1I	ACTGG	3 cut(s)	244, 352, 500
BseBI	CCWGG	3 cut(s)	122, 147, 391
BseDI	CCNNGG	2 cut(s)	121, 186
BseGI	GGATG	5 cut(s)	32, 42, 136, 334, 504
BseLI	CCNNNNNNNGG	5 cut(s)	140, 146, 187, 188, 288
BseMII	CTCAG	1 cut(s)	142
BseNI	ACTGG	3 cut(s)	244, 352, 500
BsePI	GCGCGC	1 cut(s)	456
BseRI	GAGGAG	3 cut(s)	85, 92, 479
BseSI	GKGCMC	2 cut(s)	182, 320
BseXI	GCAGC	1 cut(s)	455
BseYI	CCCAGC	1 cut(s)	15
Bsh1236I	CGCG	5 cut(s)	203, 447, 458, 466, 483
Bsh1285I	CGRYCG	2 cut(s)	186, 265
BshFI	GGCC	1 cut(s)	180
BsiEI	CGRYCG	2 cut(s)	186, 265
BsiHKAI	GWGCWC	1 cut(s)	320
BsiHKCI	CYCGRG	1 cut(s)	193
BsiSI	CCGG	2 cut(s)	72, 141
BslFI	GGGAC	1 cut(s)	210
BslI	CCNNNNNNNGG	5 cut(s)	140, 146, 187, 188, 288
BsmAI	GTCTC	3 cut(s)	34, 131, 212
BsmBI	CGTCTC	1 cut(s)	34
BsmFI	GGGAC	1 cut(s)	210
BsnI	GGCC	1 cut(s)	180
Bso31I	GGTCTC	2 cut(s)	131, 212
BsoBI	CYCGRG	1 cut(s)	193
Bsp120I	GGGCCC	1 cut(s)	178
Bsp1286I	GDGCHC	3 cut(s)	182, 194, 320
BspANI	GGCC	1 cut(s)	180
BspCNI	CTCAG	1 cut(s)	141
BspFNI	CGCG	5 cut(s)	203, 447, 458, 466, 483
BspLI	GGNNCC	1 cut(s)	180
BspTNI	GGTCTC	2 cut(s)	131, 212
BsrBI	CCGCTC	2 cut(s)	93, 429
BsrFI	RCCGGY	1 cut(s)	71
BsrI	ACTGG	3 cut(s)	244, 352, 500
BssAI	RCCGGY	1 cut(s)	71
BssECI	CCNNGG	2 cut(s)	121, 186
BssHII	GCGCGC	1 cut(s)	456
BssNI	GRCGYC	2 cut(s)	27, 273
Bst2UI	CCWGG	3 cut(s)	122, 147, 391
Bst4CI	ACNGT	2 cut(s)	187, 266
BstACI	GRCGYC	2 cut(s)	27, 273
BstBAI	YACGTR	1 cut(s)	364
BstC8I	GCNNGC	4 cut(s)	178, 231, 347, 458
BstDEI	CTNAG	1 cut(s)	128
BstDSI	CCRYGG	1 cut(s)	186
BstF5I	GGATG	5 cut(s)	32, 42, 136, 334, 504
BstFNI	CGCG	5 cut(s)	203, 447, 458, 466, 483
BstHHI	GCGC	3 cut(s)	458, 460, 468
BstMAI	GTCTC	3 cut(s)	34, 131, 212
BstMCI	CGRYCG	2 cut(s)	186, 265
BstMWI	GCNNNNNNNGC	4 cut(s)	68, 309, 423, 453
BstNI	CCWGG	3 cut(s)	122, 147, 391
BstNSI	RCATGY	1 cut(s)	349
BstSCI	CCNGG	4 cut(s)	120, 139, 145, 389
BstSLI	GKGCMC	2 cut(s)	182, 320
BstUI	CGCG	5 cut(s)	203, 447, 458, 466, 483
BstV1I	GCAGC	1 cut(s)	455
BsuRI	GGCC	1 cut(s)	180
BtgI	CCRYGG	1 cut(s)	186
BtgZI	GCGATG	2 cut(s)	43, 52
BtsCI	GGATG	5 cut(s)	32, 42, 136, 334, 504
BtsI	GCAGTG	1 cut(s)	376
BtsIMutI	CAGTG	3 cut(s)	359, 376, 493
Cac8I	GCNNGC	4 cut(s)	178, 231, 347, 458
CfoI	GCGC	3 cut(s)	458, 460, 468
Cfr10I	RCCGGY	1 cut(s)	71
Cfr13I	GGNCC	4 cut(s)	143, 178, 179, 340
CseI	GACGC	1 cut(s)	281
CviAII	CATG	1 cut(s)	346
CviJI	RGCY	8 cut(s)	19, 132, 180, 192, 229, 337, 394, 499
CviKI_1	RGCY	8 cut(s)	19, 132, 180, 192, 229, 337, 394, 499
DdeI	CTNAG	1 cut(s)	128
DrdI	GACNNNNNNGTC	1 cut(s)	204
DseDI	GACNNNNNNGTC	1 cut(s)	204
Eco24I	GRGCYC	2 cut(s)	182, 194
Eco31I	GGTCTC	2 cut(s)	131, 212
Eco47I	GGWCC	2 cut(s)	143, 340
Eco72I	CACGTG	1 cut(s)	364
Eco88I	CYCGRG	1 cut(s)	193
EcoRII	CCWGG	3 cut(s)	120, 145, 389
EcoT38I	GRGCYC	2 cut(s)	182, 194
Esp3I	CGTCTC	1 cut(s)	34
FaeI	CATG	1 cut(s)	349
FaiI	YATR	2 cut(s)	259, 347
FalI	AAGNNNNNCTT	2 cut(s)	365, 397
FaqI	GGGAC	1 cut(s)	210
FatI	CATG	1 cut(s)	345
FauI	CCCGC	3 cut(s)	169, 196, 488
Fnu4HI	GCNGC	4 cut(s)	358, 427, 454, 469
FokI	GGATG	5 cut(s)	19, 29, 123, 341, 491
FriOI	GRGCYC	2 cut(s)	182, 194
Fsp4HI	GCNGC	4 cut(s)	358, 427, 454, 469
GlaI	GCGC	3 cut(s)	457, 459, 467
GluI	GCNGC	4 cut(s)	358, 427, 454, 469
GsaI	CCCAGC	1 cut(s)	19
GsuI	CTGGAG	1 cut(s)	335
HaeIII	GGCC	1 cut(s)	180
HapII	CCGG	2 cut(s)	72, 141
HgaI	GACGC	1 cut(s)	281
HhaI	GCGC	3 cut(s)	458, 460, 468
Hin1I	GRCGYC	2 cut(s)	27, 273
Hin1II	CATG	1 cut(s)	349
Hin6I	GCGC	3 cut(s)	456, 458, 466
HinP1I	GCGC	3 cut(s)	456, 458, 466
HincII	GTYRAC	1 cut(s)	214
HindII	GTYRAC	1 cut(s)	214
HinfI	GANTC	4 cut(s)	38, 198, 386, 474
HpaI	GTTAAC	1 cut(s)	214
HpaII	CCGG	2 cut(s)	72, 141
HphI	GGTGA	1 cut(s)	97
Hpy166II	GTNNAC	2 cut(s)	214, 318
Hpy188I	TCNGA	2 cut(s)	37, 223
Hpy188III	TCNNGA	1 cut(s)	195
Hpy8I	GTNNAC	2 cut(s)	214, 318
Hpy99I	CGWCG	3 cut(s)	29, 164, 275
HpyAV	CCTTC	2 cut(s)	122, 158
HpyCH4III	ACNGT	2 cut(s)	187, 266
HpyCH4IV	ACGT	2 cut(s)	27, 363
HpyCH4V	TGCA	1 cut(s)	318
HpyF10VI	GCNNNNNNNGC	4 cut(s)	68, 309, 423, 453
HpyF3I	CTNAG	1 cut(s)	128
HpySE526I	ACGT	2 cut(s)	27, 363
Hsp92I	GRCGYC	2 cut(s)	27, 273
Hsp92II	CATG	1 cut(s)	349
HspAI	GCGC	3 cut(s)	456, 458, 466
KspAI	GTTAAC	1 cut(s)	214
LmnI	GCTCC	3 cut(s)	98, 334, 354
Lsp1109I	GCAGC	1 cut(s)	455
MaeII	ACGT	2 cut(s)	27, 363
MaeIII	GTNAC	2 cut(s)	6, 266
MbiI	CCGCTC	2 cut(s)	93, 429
MfeI	CAATTG	1 cut(s)	412
MhlI	GDGCHC	3 cut(s)	182, 194, 320
MluCI	AATT	1 cut(s)	412
MlyI	GAGTC	4 cut(s)	32, 192, 380, 468
MmeI	TCCRAC	2 cut(s)	60, 246
MseI	TTAA	1 cut(s)	213
MspA1I	CMGCKG	3 cut(s)	19, 453, 510
MspI	CCGG	2 cut(s)	72, 141
MspR9I	CCNGG	4 cut(s)	122, 141, 147, 391
MunI	CAATTG	1 cut(s)	412
MvaI	CCWGG	3 cut(s)	122, 147, 391
MvnI	CGCG	5 cut(s)	203, 447, 458, 466, 483
MwoI	GCNNNNNNNGC	4 cut(s)	68, 309, 423, 453
NciI	CCSGG	1 cut(s)	141
NlaIII	CATG	1 cut(s)	349
NlaIV	GGNNCC	1 cut(s)	180
NmuCI	GTSAC	1 cut(s)	266
NspI	RCATGY	1 cut(s)	349
PaeI	GCATGC	1 cut(s)	349
PauI	GCGCGC	1 cut(s)	456
PcsI	WCGNNNNNNNCGW	4 cut(s)	33, 165, 207, 267
PkrI	GCNGC	4 cut(s)	359, 428, 455, 470
PleI	GAGTC	4 cut(s)	32, 192, 380, 468
PmaCI	CACGTG	1 cut(s)	364
PmlI	CACGTG	1 cut(s)	364
PpsI	GAGTC	4 cut(s)	32, 192, 380, 468
Ppu21I	YACGTR	1 cut(s)	364
Psp6I	CCWGG	3 cut(s)	120, 145, 389
PspCI	CACGTG	1 cut(s)	364
PspFI	CCCAGC	1 cut(s)	15
PspGI	CCWGG	3 cut(s)	120, 145, 389
PspN4I	GGNNCC	1 cut(s)	180
PspOMI	GGGCCC	1 cut(s)	178
PspPI	GGNCC	4 cut(s)	143, 178, 179, 340
PteI	GCGCGC	1 cut(s)	456
PvuII	CAGCTG	1 cut(s)	19
SaqAI	TTAA	1 cut(s)	213
SatI	GCNGC	4 cut(s)	358, 427, 454, 469
Sau96I	GGNCC	4 cut(s)	143, 178, 179, 340
SchI	GAGTC	4 cut(s)	32, 192, 380, 468
ScrFI	CCNGG	4 cut(s)	122, 141, 147, 391
SduI	GDGCHC	3 cut(s)	182, 194, 320
SetI	ASST	9 cut(s)	21, 30, 219, 231, 323, 339, 366, 405, 489
SinI	GGWCC	2 cut(s)	143, 340
SmlI	CTYRAG	1 cut(s)	322
SmoI	CTYRAG	1 cut(s)	322
SphI	GCATGC	1 cut(s)	349
Sse9I	AATT	1 cut(s)	412
StyD4I	CCNGG	4 cut(s)	120, 139, 145, 389
TaaI	ACNGT	2 cut(s)	187, 266
TaiI	ACGT	2 cut(s)	30, 366
TaqI	TCGA	1 cut(s)	384
TasI	AATT	1 cut(s)	412
TauI	GCSGC	3 cut(s)	360, 429, 456
Tru1I	TTAA	1 cut(s)	213
Tru9I	TTAA	1 cut(s)	213
TscAI	CASTG	3 cut(s)	359, 383, 500
TseFI	GTSAC	1 cut(s)	266
TseI	GCWGC	1 cut(s)	468
Tsp45I	GTSAC	1 cut(s)	266
TspDTI	ATGAA	1 cut(s)	104
TspRI	CASTG	3 cut(s)	359, 383, 500
VneI	GTGCAC	1 cut(s)	316
VpaK11BI	GGWCC	2 cut(s)	143, 340
XceI	RCATGY	1 cut(s)	349
ZraI	GACGTC	1 cut(s)	28

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.