MD16G1073600.v1.1

Protein PHYTOCHROME KINASE SUBSTRATE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0013994
Physical Location & Seq
Forward (+)
5164867 .. 5166372
1506 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1073600.v1.1.491

Sequence Viewer

Length: 1506 bp
ATGGCTATGGTTACCTTAAAATCGACTAGCACGGCGAACATATCGCAAACTTTTCCATTCGAGAACAACGCCGCCAACCGTGATGCTTCCTTTTCGTCATACTTGAGCGGCAATGAGGAAGCCTTTGTGCGTAAACTTGCCGATCAGGCGAGCAAGGAGCAGCAGCAGCATAACATTAACAATCACTTGGGTACAAAGAAAGCAGCTGACGACGAAGAAATTGGAGTTTTTGGAGCTGAAAAGTACTTCAATGGAGTGATGGATGAGGAGACGAGCGTGACAAGCTCGAAAAGCGGCAGCAGCAGCAGCACGAAGAAATACGATCACAAGAAGGATAAACCAGTGGGTATGGATCACATGAAGATAAGGGTTCAGTCGGGGACTCCGAGTGTTCGTTCTGAATCGAGCTTGAACAGCCAAAGCGCCTTGCTTACAAGTGCGTCGAGAAATCCTTACCGGACAAACACAAGTAAGGGACACATACAGAGGAAGAACTTCTTTGCCAGCCTTGGCTGCAAATGTGTGTGTTCGGATAAGAATTCGGTTGATGTGGATGAACACGTCGGAGAAATCAGCTTCAAGAAGAACAACCATGGTGCAAACACAGGCTGCACGATAACACCAATCAATCATAAACCTCCTGACGTGGTGGACATTGTTGATGAAGTTATAGTTGATATGAAAAAAAGCGAAAAGCTTGAAAAGTTGGGAGTTGGGTTGGGAAGAGAGAACTGTTTTACTTTCCCAACTTCAAAATCCGGGGCGGGGAGTTTGCCGGCGAAGATGCTGCCTTTTCAGCAAGCAGAAGAGGAGGCGGAGAAAGTGAGGAAGTCTTTGGAAGTGTTTGGCTCCCCTGTGTTTGAGAAGCGAAATAAGTCTTTCGCCCTCGAAAAAAAGCTCAAAATGCTGCCGTGGGATGAGTTTCCGGCGAATTCTGGCCGTGTAATCTACAATAACGAGTCGGATAGTGATGCAAGTTCGGATCTTTTCGAGATTGAGAGCCTCACCGGCAAAGCAAACCCTTTTCTTGCGCGGCAAGCATCAGATGCCGGCTGCGTGACGCCAACGAATTGTTATGCGCCGAGTGAGGCGAGCATAGAGTGGAGTGTGGTCACTGCCAGCGTTGCTGACATCTCCATGATGTCCGATTTCGAAGATCACCAGAGACCAGCGAAAATGGCTCCAAATTCTACGAATGCCAAAGCAAGAATGACCAAGGAAATTCCGAGGCACCGCTCCGGGGCGCTGTTGGGTTGTAAGAGCCAGAAATCTGTGAAAGTTGCCGGAGATGCACACAGAAGTACGTATCAACATGAGAAGAGCTATTTCGAACAGCAGGTCACGCACCGCAGGGCGGAATCATACGTGCCAGGGACAAGGTTTCAAGCGGAGACAACTAATAAGCTGACAGGATTTGATGCAAGAGTCCAAGGGCAGCGTGCTCTTGCAGCACGGCCGCTCCCTCGTTCACATTCACCGCACGCTTCACATCTTTTGTACATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

502

Amino Acids

55.08

Weight (kDa)

8.69

Isoelectric Point (pI)

43.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013994)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14280 AT2G02950
fragaria_vesca FvH4_4g31450
malus_domestica MD16G1073600.v1.1
prunus_persica Prupe.1G273300_v2.0.a1
pyrus_communis pycom13g06530
rosa_chinensis RchiOBHm_Chr4g0439931
rosa_laevigata RLG00000006224
rosa_multiflora Rmu_sc0006000.1_g000001 Rmu_sc0022459.1_g000001
rosa_roxburghii Rroxscaffold_5G00380880
rosa_rugosa Rorug04G0322600
rosa_samantha Rh4AG373500 Rh4BG385800 Rh4CG400300 Rh4DG380100
rosa_wichuraiana Rw4G031940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1327
AccB1I GGYRCC 1 cut(s) 1230
AccBSI CCGCTC 3 cut(s) 108, 1236, 1459
AccII CGCG 1 cut(s) 1033
AclWI GGATC 2 cut(s) 360, 990
AcoI YGGCCR 2 cut(s) 937, 1454
AcsI RAATTY 4 cut(s) 538, 931, 1186, 1221
AcyI GRCGYC 1 cut(s) 1061
AfaI GTAC 4 cut(s) 193, 245, 1303, 1499
AfiI CCNNNNNNNGG 3 cut(s) 765, 1240, 1354
AflIII ACRYGT 2 cut(s) 559, 1500
AgsI TTSAA 6 cut(s) 250, 412, 580, 701, 753, 1385
AjiI CACGTC 2 cut(s) 562, 646
AjnI CCWGG 1 cut(s) 1369
AluBI AGCT 9 cut(s) 206, 236, 285, 408, 576, 697, 898, 1323, 1405
AluI AGCT 9 cut(s) 206, 236, 285, 408, 576, 697, 898, 1323, 1405
Alw21I GWGCWC 1 cut(s) 1444
Alw26I GTCTC 3 cut(s) 263, 1159, 1385
AlwI GGATC 2 cut(s) 360, 990
AoxI GGCC 2 cut(s) 937, 1454
ApoI RAATTY 4 cut(s) 538, 931, 1186, 1221
ArsI GACNNNNNNTTYG 2 cut(s) 1323, 1355
Asp700I GAANNNNTTC 1 cut(s) 494
AspLEI GCGC 4 cut(s) 425, 1033, 1081, 1246
AsuC2I CCSGG 2 cut(s) 760, 1240
AsuHPI GGTGA 3 cut(s) 997, 1151, 1467
AsuII TTCGAA 2 cut(s) 1152, 1329
BanI GGYRCC 1 cut(s) 1230
Bbv12I GWGCWC 1 cut(s) 1444
BccI CCATC 1 cut(s) 253
BceAI ACGGC 4 cut(s) 48, 895, 924, 1469
BcgI CGANNNNNNTGC 2 cut(s) 769, 803
BciT130I CCWGG 1 cut(s) 1371
BcnI CCSGG 2 cut(s) 760, 1240
BcoDI GTCTC 3 cut(s) 263, 1159, 1385
BfaI CTAG 1 cut(s) 27
BfoI RGCGCY 2 cut(s) 426, 1247
BfuAI ACCTGC 1 cut(s) 1327
BglI GCCNNNNNGGC 2 cut(s) 146, 1008
BmcAI AGTACT 1 cut(s) 245
Bme1390I CCNGG 3 cut(s) 760, 1240, 1371
BmgBI CACGTC 2 cut(s) 562, 646
BmiI GGNNCC 3 cut(s) 850, 1182, 1232
BmrFI CCNGG 3 cut(s) 760, 1240, 1371
BmsI GCATC 7 cut(s) 73, 774, 961, 1036, 1049, 1279, 1408
Bpu14I TTCGAA 2 cut(s) 1152, 1329
BpuEI CTTGAG 1 cut(s) 124
BpuMI CCSGG 2 cut(s) 760, 1240
BsaAI YACGTR 2 cut(s) 1305, 1366
BsaHI GRCGYC 1 cut(s) 1061
BsaI GGTCTC 1 cut(s) 1159
BsaJI CCNNGG 9 cut(s) 508, 592, 759, 911, 1215, 1226, 1239, 1370, 1429
BsaWI WCCGGW 1 cut(s) 456
BsaXI ACNNNNNCTCC 2 cut(s) 1443, 1473
Bsc4I CCNNNNNNNGG 3 cut(s) 765, 1240, 1354
Bse118I RCCGGY 3 cut(s) 775, 1007, 1049
Bse1I ACTGG 1 cut(s) 341
Bse3DI GCAATG 1 cut(s) 118
BseBI CCWGG 1 cut(s) 1371
BseDI CCNNGG 9 cut(s) 508, 592, 759, 911, 1215, 1226, 1239, 1370, 1429
BseGI GGATG 3 cut(s) 268, 559, 922
BseLI CCNNNNNNNGG 3 cut(s) 765, 1240, 1354
BseMI GCAATG 1 cut(s) 118
BseNI ACTGG 1 cut(s) 341
BseRI GAGGAG 2 cut(s) 281, 824
BseX3I CGGCCG 1 cut(s) 1454
BsgI GTGCAG 1 cut(s) 595
Bsh1236I CGCG 1 cut(s) 1033
Bsh1285I CGRYCG 1 cut(s) 1457
BshFI GGCC 2 cut(s) 939, 1456
BshNI GGYRCC 1 cut(s) 1230
BsiEI CGRYCG 1 cut(s) 1457
BsiHKAI GWGCWC 1 cut(s) 1444
BsiSI CCGG 8 cut(s) 457, 759, 776, 926, 1008, 1050, 1239, 1284
BslFI GGGAC 3 cut(s) 394, 489, 1387
BslI CCNNNNNNNGG 3 cut(s) 765, 1240, 1354
BsmAI GTCTC 3 cut(s) 263, 1159, 1385
BsmBI CGTCTC 1 cut(s) 263
BsmFI GGGAC 3 cut(s) 394, 489, 1387
BsmI GAATGC 1 cut(s) 1201
BsnI GGCC 2 cut(s) 939, 1456
Bso31I GGTCTC 1 cut(s) 1159
Bsp119I TTCGAA 2 cut(s) 1152, 1329
Bsp1286I GDGCHC 1 cut(s) 1444
Bsp1407I TGTACA 1 cut(s) 1497
Bsp143I GATC 5 cut(s) 142, 322, 352, 982, 1156
Bsp19I CCATGG 1 cut(s) 592
BspANI GGCC 2 cut(s) 939, 1456
BspFNI CGCG 1 cut(s) 1033
BspLI GGNNCC 3 cut(s) 850, 1182, 1232
BspMI ACCTGC 1 cut(s) 1327
BspPI GGATC 2 cut(s) 360, 990
BspQI GCTCTTC 1 cut(s) 1313
BspT104I TTCGAA 2 cut(s) 1152, 1329
BspT107I GGYRCC 1 cut(s) 1230
BspTNI GGTCTC 1 cut(s) 1159
BsrBI CCGCTC 3 cut(s) 108, 1236, 1459
BsrDI GCAATG 1 cut(s) 118
BsrFI RCCGGY 3 cut(s) 775, 1007, 1049
BsrGI TGTACA 1 cut(s) 1497
BsrI ACTGG 1 cut(s) 341
BssAI RCCGGY 3 cut(s) 775, 1007, 1049
BssECI CCNNGG 9 cut(s) 508, 592, 759, 911, 1215, 1226, 1239, 1370, 1429
BssMI GATC 5 cut(s) 142, 322, 352, 982, 1156
BssNI GRCGYC 1 cut(s) 1061
BssT1I CCWWGG 4 cut(s) 508, 592, 1215, 1429
Bst2UI CCWGG 1 cut(s) 1371
Bst4CI ACNGT 2 cut(s) 80, 734
Bst6I CTCTTC 3 cut(s) 718, 801, 1313
BstACI GRCGYC 1 cut(s) 1061
BstAPI GCANNNNNTGC 1 cut(s) 1046
BstAUI TGTACA 1 cut(s) 1497
BstBAI YACGTR 2 cut(s) 1305, 1366
BstBI TTCGAA 2 cut(s) 1152, 1329
BstDSI CCRYGG 2 cut(s) 592, 911
BstEII GGTNACC 1 cut(s) 10
BstF5I GGATG 3 cut(s) 268, 559, 922
BstFNI CGCG 1 cut(s) 1033
BstH2I RGCGCY 2 cut(s) 426, 1247
BstHHI GCGC 4 cut(s) 425, 1033, 1081, 1246
BstKTI GATC 5 cut(s) 145, 325, 355, 985, 1159
BstMAI GTCTC 3 cut(s) 263, 1159, 1385
BstMBI GATC 5 cut(s) 142, 322, 352, 982, 1156
BstMCI CGRYCG 1 cut(s) 1457
BstNI CCWGG 1 cut(s) 1371
BstNSI RCATGY 1 cut(s) 1504
BstPI GGTNACC 1 cut(s) 10
BstSCI CCNGG 3 cut(s) 758, 1238, 1369
BstSNI TACGTA 1 cut(s) 1305
BstUI CGCG 1 cut(s) 1033
BstX2I RGATCY 1 cut(s) 982
BstYI RGATCY 1 cut(s) 982
BstZI CGGCCG 1 cut(s) 1454
BsuRI GGCC 2 cut(s) 939, 1456
BtgI CCRYGG 2 cut(s) 592, 911
BtrI CACGTC 2 cut(s) 562, 646
BtsCI GGATG 3 cut(s) 268, 559, 922
BtsI GCAGTG 1 cut(s) 1113
BtsIMutI CAGTG 2 cut(s) 348, 1113
BveI ACCTGC 1 cut(s) 1327
CfoI GCGC 4 cut(s) 425, 1033, 1081, 1246
Cfr10I RCCGGY 3 cut(s) 775, 1007, 1049
CseI GACGC 2 cut(s) 429, 1069
Csp6I GTAC 4 cut(s) 192, 244, 1302, 1498
CviAII CATG 5 cut(s) 358, 593, 1138, 1313, 1501
CviQI GTAC 4 cut(s) 192, 244, 1302, 1498
DpnI GATC 5 cut(s) 144, 324, 354, 984, 1158
DpnII GATC 5 cut(s) 142, 322, 352, 982, 1156
EaeI YGGCCR 2 cut(s) 937, 1454
EagI CGGCCG 1 cut(s) 1454
Eam1104I CTCTTC 3 cut(s) 718, 801, 1313
EarI CTCTTC 3 cut(s) 718, 801, 1313
EciI GGCGGA 2 cut(s) 830, 1370
EclXI CGGCCG 1 cut(s) 1454
Eco105I TACGTA 1 cut(s) 1305
Eco130I CCWWGG 4 cut(s) 508, 592, 1215, 1429
Eco31I GGTCTC 1 cut(s) 1159
Eco52I CGGCCG 1 cut(s) 1454
Eco91I GGTNACC 1 cut(s) 10
EcoO65I GGTNACC 1 cut(s) 10
EcoRI GAATTC 2 cut(s) 538, 931
EcoRII CCWGG 1 cut(s) 1369
EcoT14I CCWWGG 4 cut(s) 508, 592, 1215, 1429
ErhI CCWWGG 4 cut(s) 508, 592, 1215, 1429
Esp3I CGTCTC 1 cut(s) 263
FaeI CATG 5 cut(s) 361, 596, 1141, 1316, 1504
FalI AAGNNNNNCTT 2 cut(s) 482, 514
FaqI GGGAC 3 cut(s) 394, 489, 1387
FatI CATG 5 cut(s) 357, 592, 1137, 1312, 1500
FauI CCCGC 1 cut(s) 757
FokI GGATG 3 cut(s) 275, 566, 929
FspBI CTAG 1 cut(s) 27
GlaI GCGC 4 cut(s) 424, 1032, 1080, 1245
HaeII RGCGCY 2 cut(s) 426, 1247
HaeIII GGCC 2 cut(s) 939, 1456
HapII CCGG 8 cut(s) 457, 759, 776, 926, 1008, 1050, 1239, 1284
HgaI GACGC 2 cut(s) 429, 1069
HhaI GCGC 4 cut(s) 425, 1033, 1081, 1246
Hin1I GRCGYC 1 cut(s) 1061
Hin1II CATG 5 cut(s) 361, 596, 1141, 1316, 1504
Hin6I GCGC 4 cut(s) 423, 1031, 1079, 1244
HinP1I GCGC 4 cut(s) 423, 1031, 1079, 1244
HindIII AAGCTT 1 cut(s) 695
HinfI GANTC 5 cut(s) 382, 401, 959, 1358, 1425
HpaII CCGG 8 cut(s) 457, 759, 776, 926, 1008, 1050, 1239, 1284
HphI GGTGA 3 cut(s) 997, 1151, 1467
Hpy166II GTNNAC 3 cut(s) 134, 652, 1469
Hpy188I TCNGA 9 cut(s) 387, 400, 532, 566, 964, 982, 1045, 1147, 1227
Hpy188III TCNNGA 5 cut(s) 61, 444, 580, 641, 991
Hpy8I GTNNAC 3 cut(s) 134, 652, 1469
Hpy99I CGWCG 3 cut(s) 215, 445, 566
HpyAV CCTTC 1 cut(s) 325
HpyCH4III ACNGT 2 cut(s) 80, 734
HpyCH4IV ACGT 4 cut(s) 561, 645, 1304, 1365
HpyCH4V TGCA 7 cut(s) 516, 599, 612, 974, 1292, 1421, 1448
HpySE526I ACGT 4 cut(s) 561, 645, 1304, 1365
Hsp92I GRCGYC 1 cut(s) 1061
Hsp92II CATG 5 cut(s) 361, 596, 1141, 1316, 1504
HspAI GCGC 4 cut(s) 423, 1031, 1079, 1244
KroI GCCGGC 2 cut(s) 775, 1049
KroNI GCCGGC 2 cut(s) 777, 1051
Kzo9I GATC 5 cut(s) 142, 322, 352, 982, 1156
LguI GCTCTTC 1 cut(s) 1313
LmnI GCTCC 6 cut(s) 157, 233, 854, 1186, 1241, 1464
LweI GCATC 7 cut(s) 73, 774, 961, 1036, 1049, 1279, 1408
MaeI CTAG 1 cut(s) 27
MaeII ACGT 4 cut(s) 561, 645, 1304, 1365
MaeIII GTNAC 5 cut(s) 10, 277, 1057, 1111, 1339
MalI GATC 5 cut(s) 144, 324, 354, 984, 1158
MbiI CCGCTC 3 cut(s) 108, 1236, 1459
MboI GATC 5 cut(s) 142, 322, 352, 982, 1156
MflI RGATCY 1 cut(s) 982
MhlI GDGCHC 1 cut(s) 1444
MluCI AATT 6 cut(s) 219, 538, 931, 1069, 1186, 1221
MlyI GAGTC 3 cut(s) 376, 968, 1434
MmeI TCCRAC 2 cut(s) 544, 942
MroNI GCCGGC 2 cut(s) 775, 1049
MroXI GAANNNNTTC 1 cut(s) 494
MseI TTAA 2 cut(s) 17, 177
MslI CAYNNNNRTG 1 cut(s) 1136
MspA1I CMGCKG 1 cut(s) 206
MspI CCGG 8 cut(s) 457, 759, 776, 926, 1008, 1050, 1239, 1284
MspR9I CCNGG 3 cut(s) 760, 1240, 1371
Mva1269I GAATGC 1 cut(s) 1201
MvaI CCWGG 1 cut(s) 1371
MvnI CGCG 1 cut(s) 1033
NaeI GCCGGC 2 cut(s) 777, 1051
NciI CCSGG 2 cut(s) 760, 1240
NcoI CCATGG 1 cut(s) 592
NdeII GATC 5 cut(s) 142, 322, 352, 982, 1156
NgoMIV GCCGGC 2 cut(s) 775, 1049
NlaIII CATG 5 cut(s) 361, 596, 1141, 1316, 1504
NlaIV GGNNCC 3 cut(s) 850, 1182, 1232
NmeAIII GCCGAG 1 cut(s) 1107
NmuCI GTSAC 4 cut(s) 277, 1057, 1111, 1339
NspI RCATGY 1 cut(s) 1504
NspV TTCGAA 2 cut(s) 1152, 1329
PciI ACATGT 1 cut(s) 1500
PciSI GCTCTTC 1 cut(s) 1313
PcsI WCGNNNNNNNCGW 1 cut(s) 383
PctI GAATGC 1 cut(s) 1201
PdiI GCCGGC 2 cut(s) 777, 1051
PdmI GAANNNNTTC 1 cut(s) 494
PfeI GAWTC 2 cut(s) 401, 1358
PleI GAGTC 3 cut(s) 376, 967, 1433
PpsI GAGTC 3 cut(s) 376, 967, 1433
Ppu21I YACGTR 2 cut(s) 1305, 1366
PscI ACATGT 1 cut(s) 1500
Psp6I CCWGG 1 cut(s) 1369
PspEI GGTNACC 1 cut(s) 10
PspGI CCWGG 1 cut(s) 1369
PspN4I GGNNCC 3 cut(s) 850, 1182, 1232
PsuI RGATCY 1 cut(s) 982
PvuII CAGCTG 1 cut(s) 206
RsaI GTAC 4 cut(s) 193, 245, 1303, 1499
RsaNI GTAC 4 cut(s) 192, 244, 1302, 1498
RseI CAYNNNNRTG 1 cut(s) 1136
SapI GCTCTTC 1 cut(s) 1313
SaqAI TTAA 2 cut(s) 17, 177
Sau3AI GATC 5 cut(s) 142, 322, 352, 982, 1156
ScaI AGTACT 1 cut(s) 245
SchI GAGTC 3 cut(s) 376, 968, 1434
ScrFI CCNGG 3 cut(s) 760, 1240, 1371
SduI GDGCHC 1 cut(s) 1444
SfaNI GCATC 7 cut(s) 73, 774, 961, 1036, 1049, 1279, 1408
SfuI TTCGAA 2 cut(s) 1152, 1329
SmiMI CAYNNNNRTG 1 cut(s) 1136
SmlI CTYRAG 1 cut(s) 103
SmoI CTYRAG 1 cut(s) 103
SnaBI TACGTA 1 cut(s) 1305
Sse9I AATT 6 cut(s) 219, 538, 931, 1069, 1186, 1221
SspMI CTAG 1 cut(s) 27
StyD4I CCNGG 3 cut(s) 758, 1238, 1369
StyI CCWWGG 4 cut(s) 508, 592, 1215, 1429
TaaI ACNGT 2 cut(s) 80, 734
TaiI ACGT 4 cut(s) 564, 648, 1307, 1368
TaqI TCGA 9 cut(s) 23, 60, 287, 404, 443, 888, 990, 1152, 1329
TasI AATT 6 cut(s) 219, 538, 931, 1069, 1186, 1221
TatI WGTACW 2 cut(s) 243, 1497
TauI GCSGC 5 cut(s) 74, 111, 297, 1036, 1459
TfiI GAWTC 2 cut(s) 401, 1358
Tru1I TTAA 2 cut(s) 17, 177
Tru9I TTAA 2 cut(s) 17, 177
TscAI CASTG 2 cut(s) 348, 1120
TseFI GTSAC 4 cut(s) 277, 1057, 1111, 1339
Tsp45I GTSAC 4 cut(s) 277, 1057, 1111, 1339
TspDTI ATGAA 4 cut(s) 374, 570, 678, 695
TspRI CASTG 2 cut(s) 348, 1120
XapI RAATTY 4 cut(s) 538, 931, 1186, 1221
XceI RCATGY 1 cut(s) 1504
XmnI GAANNNNTTC 1 cut(s) 494
XspI CTAG 1 cut(s) 27
ZrmI AGTACT 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.