Prupe.1G273300_v2.0.a1

phytochrome kinase substrate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0013994
Physical Location & Seq
Reverse (-)
27974515 .. 27975948
1434 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G273300.1

Sequence Viewer

Length: 1434 bp
ATGGCTATGGTTACCTTAAAATCAACTAGCAAGGCCAACCTCTCACAAACGTTTCCCTGTGACAACAACAAGAACCGTGATGCCTCCTTTTCTTCATACTTGAGTAGCAATGAGGAAACCCTTGTACGTAAACTTGTTGATCAGTCAAGTGAGGAACATAACAATCACTTGGCAACAAAGAAGGAAGAAGATGAAGAAATTGGAGTATTTGGAGCTGAAAAGTACTTCAATGGAGTAATGGACGAGGAGACTAGCCCAAAAAGCGGCAGCAGCTCAAAGAATTACCATCATAACAAAGAGAAACGAGTTCAACCTGGAACTCCAAGTGTTCGTTCTGAATCAAGTTGGAACAGCCAAACTTCGCTATTGCAAAGCCGGTTGAGAAATCCTTATCAGAGTAATACAAGCAGGGCACAGAGACAGAAGAAGAACTTTTTTGCAAGCCTTGGTTGCAAATGCTCTTGTTCGGATAAGAATTCGGTCGATGTCGATGAACATGTTGGTGAAATCAGCTTCAAGAAGAATAATGGTGCTGCTAACAGTGGAACATTGGTCCATGGCAAAGCAAGCACAACAAGCACCATTAACAATAAACCTGCAGCTGTAGTAGACCTTGTTGGTGAAGCTCTAGTTGAGATGAACAACAGCCAAAAGCTTGATAAGTTGGGAGTTGGGTTGAGCAGAGAAAACTGTTTTACTTTCCCATCTTCAAAACCTGGGATGGGGAGTTTGCCAGTGAAGATGCCCTTTCAAGAAGAAGAAGAAGCAGAGAAAGTGAGGAAATCTCTGGAGGTATTTGGTTCTCCTGTGTTTGAGAAAAGGAACAGGTCTTTAGGCCTTGACAAAAGGCTAACAATGTTGCCTTGGGATGAGTTTCCTGCAAACTCCGGTGGAGTGATATACAATAATGAGTCAGATAGTGATGCAAGTTCAGATCTTTTTGAGATAGAGAGCCTCACAGGCAAAGCCAACCCTTGCATGACCCCAACAACTTGTTATGCCCCAAGTGAGGCAAGCATAGAGTGGAGTGTGGCCACTGCCAGTGTTGCTGATTTCTCTGTGACGTCAGACTCTGAAGATCTGCAAAGACTAAGTCCAATGAAAATGGTTCCAAACAACACCAGAAATGCCAAAACAAGAATGAACAAGGAGATTCCAAGGCATCGTCCCACGGCTCTATTGGGTTGTAAGAGCCAAAAAGCTGTCAAAATTGCAGGAGATGCCCACAAAACATATCATCATGAGAAGACCAACTTTGACCAACAGATGATGCGCCGCAACAGGCCAGAATCGTTCGTGCCAGCAACAAGGTTTCAAGCTGAGACGTCCCCCAATAAGTTGACAGGCTTTGACTCAAGAGTACAAGGGCAGCATGCTCTTGCAGCACAGTCTCGTTCGCATTCACCACATGCCTCACATCTTTTGTTCATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

478

Amino Acids

52.78

Weight (kDa)

8.47

Isoelectric Point (pI)

58.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013994)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14280 AT2G02950
fragaria_vesca FvH4_4g31450
malus_domestica MD16G1073600.v1.1
prunus_persica Prupe.1G273300_v2.0.a1
pyrus_communis pycom13g06530
rosa_chinensis RchiOBHm_Chr4g0439931
rosa_laevigata RLG00000006224
rosa_multiflora Rmu_sc0006000.1_g000001 Rmu_sc0022459.1_g000001
rosa_roxburghii Rroxscaffold_5G00380880
rosa_rugosa Rorug04G0322600
rosa_samantha Rh4AG373500 Rh4BG385800 Rh4CG400300 Rh4DG380100
rosa_wichuraiana Rw4G031940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 1067, 1328
Acc36I ACCTGC 1 cut(s) 604
AccI GTMKAC 1 cut(s) 609
AciI CCGC 2 cut(s) 264, 1276
AclI AACGTT 1 cut(s) 50
AcoI YGGCCR 1 cut(s) 1032
AcsI RAATTY 1 cut(s) 475
AcuI CTGAAG 1 cut(s) 1095
AcyI GRCGYC 2 cut(s) 1064, 1325
AfaI GTAC 3 cut(s) 126, 224, 1362
AfiI CCNNNNNNNGG 3 cut(s) 263, 722, 1009
AflIII ACRYGT 1 cut(s) 496
AgsI TTSAA 6 cut(s) 229, 311, 517, 711, 752, 1316
AjnI CCWGG 2 cut(s) 313, 715
AjuI GAANNNNNNNTTGG 2 cut(s) 316, 348
AluBI AGCT 8 cut(s) 215, 273, 513, 602, 626, 655, 1202, 1319
AluI AGCT 8 cut(s) 215, 273, 513, 602, 626, 655, 1202, 1319
Alw26I GTCTC 4 cut(s) 242, 412, 1316, 1395
AlwNI CAGNNNCTG 1 cut(s) 1073
AoxI GGCC 4 cut(s) 33, 835, 1032, 1283
ApeKI GCWGC 6 cut(s) 267, 270, 533, 599, 1369, 1382
ApoI RAATTY 1 cut(s) 475
ArsI GACNNNNNNTTYG 2 cut(s) 1078, 1110
AspLEI GCGC 1 cut(s) 1275
AspS9I GGNCC 1 cut(s) 553
AsuHPI GGTGA 3 cut(s) 515, 632, 1395
AvaII GGWCC 1 cut(s) 553
BaeGI GKGCMC 1 cut(s) 415
BalI TGGCCA 1 cut(s) 1034
BbsI GAAGAC 1 cut(s) 1253
BbvI GCAGC 6 cut(s) 279, 282, 520, 611, 1381, 1394
BccI CCATC 3 cut(s) 294, 712, 715
BceAI ACGGC 1 cut(s) 1188
BciT130I CCWGG 2 cut(s) 315, 717
BclI TGATCA 1 cut(s) 139
BcoDI GTCTC 4 cut(s) 242, 412, 1316, 1395
BfaI CTAG 3 cut(s) 27, 252, 629
BfmI CTRYAG 2 cut(s) 597, 603
BfuAI ACCTGC 1 cut(s) 604
BglI GCCNNNNNGGC 1 cut(s) 960
BglII AGATCT 2 cut(s) 934, 1078
BisI GCNGC 8 cut(s) 265, 268, 271, 534, 600, 1276, 1370, 1383
BlsI GCNGC 8 cut(s) 266, 269, 272, 535, 601, 1277, 1371, 1384
BmcAI AGTACT 1 cut(s) 224
Bme1390I CCNGG 2 cut(s) 315, 717
Bme18I GGWCC 1 cut(s) 553
BmgT120I GGNCC 1 cut(s) 553
BmiI GGNNCC 1 cut(s) 1110
BmrFI CCNGG 2 cut(s) 315, 717
BmsI GCATC 6 cut(s) 70, 732, 913, 1171, 1210, 1260
BpiI GAAGAC 1 cut(s) 1253
BplI GAGNNNNNCTC 2 cut(s) 769, 801
BpmI CTGGAG 1 cut(s) 809
BpuEI CTTGAG 2 cut(s) 121, 1339
BsaAI YACGTR 1 cut(s) 128
BsaHI GRCGYC 2 cut(s) 1064, 1325
BsaJI CCNNGG 6 cut(s) 445, 556, 716, 863, 1157, 1170
BsaWI WCCGGW 1 cut(s) 887
Bsc4I CCNNNNNNNGG 3 cut(s) 263, 722, 1009
Bse118I RCCGGY 1 cut(s) 375
Bse1I ACTGG 2 cut(s) 734, 1041
Bse3DI GCAATG 1 cut(s) 115
BseBI CCWGG 2 cut(s) 315, 717
BseDI CCNNGG 6 cut(s) 445, 556, 716, 863, 1157, 1170
BseGI GGATG 2 cut(s) 726, 874
BseLI CCNNNNNNNGG 3 cut(s) 263, 722, 1009
BseMI GCAATG 1 cut(s) 115
BseMII CTCAG 1 cut(s) 1311
BseNI ACTGG 2 cut(s) 734, 1041
BseRI GAGGAG 1 cut(s) 260
BseSI GKGCMC 1 cut(s) 415
BseXI GCAGC 6 cut(s) 279, 282, 520, 611, 1381, 1394
Bsh1285I CGRYCG 1 cut(s) 483
BshFI GGCC 4 cut(s) 35, 837, 1034, 1285
BsiEI CGRYCG 1 cut(s) 483
BsiSI CCGG 2 cut(s) 376, 888
BslFI GGGAC 2 cut(s) 1152, 1312
BslI CCNNNNNNNGG 3 cut(s) 263, 722, 1009
BsmAI GTCTC 4 cut(s) 242, 412, 1316, 1395
BsmBI CGTCTC 1 cut(s) 1316
BsmFI GGGAC 2 cut(s) 1152, 1312
BsmI GAATGC 1 cut(s) 1399
BsnI GGCC 4 cut(s) 35, 837, 1034, 1285
Bsp1286I GDGCHC 1 cut(s) 415
Bsp143I GATC 3 cut(s) 139, 934, 1078
Bsp19I CCATGG 1 cut(s) 556
BspACI CCGC 2 cut(s) 264, 1276
BspANI GGCC 4 cut(s) 35, 837, 1034, 1285
BspCNI CTCAG 1 cut(s) 1312
BspHI TCATGA 1 cut(s) 1240
BspLI GGNNCC 1 cut(s) 1110
BspMAI CTGCAG 1 cut(s) 601
BspMI ACCTGC 1 cut(s) 604
BsrDI GCAATG 1 cut(s) 115
BsrFI RCCGGY 1 cut(s) 375
BsrI ACTGG 2 cut(s) 734, 1041
BssAI RCCGGY 1 cut(s) 375
BssECI CCNNGG 6 cut(s) 445, 556, 716, 863, 1157, 1170
BssMI GATC 3 cut(s) 139, 934, 1078
BssNI GRCGYC 2 cut(s) 1064, 1325
BssT1I CCWWGG 4 cut(s) 445, 556, 863, 1157
Bst2UI CCWGG 2 cut(s) 315, 717
Bst4CI ACNGT 4 cut(s) 77, 542, 692, 1389
BstACI GRCGYC 2 cut(s) 1064, 1325
BstAPI GCANNNNNTGC 1 cut(s) 1220
BstBAI YACGTR 1 cut(s) 128
BstC8I GCNNGC 5 cut(s) 442, 568, 1015, 1302, 1374
BstDEI CTNAG 2 cut(s) 1091, 1320
BstDSI CCRYGG 2 cut(s) 556, 1170
BstEII GGTNACC 1 cut(s) 10
BstF5I GGATG 2 cut(s) 726, 874
BstHHI GCGC 1 cut(s) 1275
BstKTI GATC 3 cut(s) 142, 937, 1081
BstMAI GTCTC 4 cut(s) 242, 412, 1316, 1395
BstMBI GATC 3 cut(s) 139, 934, 1078
BstMCI CGRYCG 1 cut(s) 483
BstMWI GCNNNNNNNGC 9 cut(s) 261, 270, 450, 567, 576, 960, 1046, 1220, 1382
BstNI CCWGG 2 cut(s) 315, 717
BstNSI RCATGY 3 cut(s) 500, 1376, 1412
BstPI GGTNACC 1 cut(s) 10
BstSCI CCNGG 2 cut(s) 313, 715
BstSFI CTRYAG 2 cut(s) 597, 603
BstSLI GKGCMC 1 cut(s) 415
BstSNI TACGTA 1 cut(s) 128
BstV1I GCAGC 6 cut(s) 279, 282, 520, 611, 1381, 1394
BstV2I GAAGAC 1 cut(s) 1253
BstX2I RGATCY 2 cut(s) 934, 1078
BstYI RGATCY 2 cut(s) 934, 1078
BsuRI GGCC 4 cut(s) 35, 837, 1034, 1285
BtgI CCRYGG 2 cut(s) 556, 1170
BtsCI GGATG 2 cut(s) 726, 874
BtsI GCAGTG 1 cut(s) 1035
BtsIMutI CAGTG 4 cut(s) 547, 741, 1035, 1048
BveI ACCTGC 1 cut(s) 604
Cac8I GCNNGC 5 cut(s) 442, 568, 1015, 1302, 1374
CaiI CAGNNNCTG 1 cut(s) 1073
CciI TCATGA 1 cut(s) 1240
CfoI GCGC 1 cut(s) 1275
Cfr10I RCCGGY 1 cut(s) 375
Cfr13I GGNCC 1 cut(s) 553
Csp6I GTAC 3 cut(s) 125, 223, 1361
CviAII CATG 7 cut(s) 497, 557, 979, 1241, 1373, 1409, 1429
CviQI GTAC 3 cut(s) 125, 223, 1361
DdeI CTNAG 2 cut(s) 1091, 1320
DpnI GATC 3 cut(s) 141, 936, 1080
DpnII GATC 3 cut(s) 139, 934, 1078
EaeI YGGCCR 1 cut(s) 1032
Eco105I TACGTA 1 cut(s) 128
Eco130I CCWWGG 4 cut(s) 445, 556, 863, 1157
Eco147I AGGCCT 1 cut(s) 837
Eco47I GGWCC 1 cut(s) 553
Eco57I CTGAAG 1 cut(s) 1095
Eco91I GGTNACC 1 cut(s) 10
EcoO65I GGTNACC 1 cut(s) 10
EcoRI GAATTC 1 cut(s) 475
EcoRII CCWGG 2 cut(s) 313, 715
EcoT14I CCWWGG 4 cut(s) 445, 556, 863, 1157
ErhI CCWWGG 4 cut(s) 445, 556, 863, 1157
Esp3I CGTCTC 1 cut(s) 1316
FaeI CATG 7 cut(s) 500, 560, 982, 1244, 1376, 1412, 1432
FalI AAGNNNNNCTT 6 cut(s) 416, 448, 731, 763, 1238, 1270
FaqI GGGAC 2 cut(s) 1152, 1312
FatI CATG 7 cut(s) 496, 556, 978, 1240, 1372, 1408, 1428
FbaI TGATCA 1 cut(s) 139
FblI GTMKAC 1 cut(s) 609
Fnu4HI GCNGC 8 cut(s) 265, 268, 271, 534, 600, 1276, 1370, 1383
FokI GGATG 2 cut(s) 733, 881
Fsp4HI GCNGC 8 cut(s) 265, 268, 271, 534, 600, 1276, 1370, 1383
FspBI CTAG 3 cut(s) 27, 252, 629
GlaI GCGC 1 cut(s) 1274
GluI GCNGC 8 cut(s) 265, 268, 271, 534, 600, 1276, 1370, 1383
GsuI CTGGAG 1 cut(s) 809
HaeIII GGCC 4 cut(s) 35, 837, 1034, 1285
HapII CCGG 2 cut(s) 376, 888
HhaI GCGC 1 cut(s) 1275
Hin1I GRCGYC 2 cut(s) 1064, 1325
Hin1II CATG 7 cut(s) 500, 560, 982, 1244, 1376, 1412, 1432
Hin6I GCGC 1 cut(s) 1273
HinP1I GCGC 1 cut(s) 1273
HincII GTYRAC 1 cut(s) 1341
HindII GTYRAC 1 cut(s) 1341
HindIII AAGCTT 1 cut(s) 653
HinfI GANTC 6 cut(s) 338, 911, 1070, 1153, 1289, 1352
HpaII CCGG 2 cut(s) 376, 888
HphI GGTGA 3 cut(s) 515, 632, 1395
Hpy166II GTNNAC 3 cut(s) 131, 610, 1341
Hpy188I TCNGA 7 cut(s) 337, 396, 469, 916, 934, 1069, 1075
Hpy188III TCNNGA 5 cut(s) 517, 752, 788, 1241, 1356
Hpy8I GTNNAC 3 cut(s) 131, 610, 1341
HpyAV CCTTC 1 cut(s) 175
HpyCH4III ACNGT 4 cut(s) 77, 542, 692, 1389
HpyCH4IV ACGT 4 cut(s) 50, 127, 1064, 1325
HpyF10VI GCNNNNNNNGC 9 cut(s) 261, 270, 450, 567, 576, 960, 1046, 1220, 1382
HpyF3I CTNAG 2 cut(s) 1091, 1320
HpySE526I ACGT 4 cut(s) 50, 127, 1064, 1325
Hsp92I GRCGYC 2 cut(s) 1064, 1325
Hsp92II CATG 7 cut(s) 500, 560, 982, 1244, 1376, 1412, 1432
HspAI GCGC 1 cut(s) 1273
Ksp22I TGATCA 1 cut(s) 139
Kzo9I GATC 3 cut(s) 139, 934, 1078
LmnI GCTCC 1 cut(s) 212
Lsp1109I GCAGC 6 cut(s) 279, 282, 520, 611, 1381, 1394
LweI GCATC 6 cut(s) 70, 732, 913, 1171, 1210, 1260
MaeI CTAG 3 cut(s) 27, 252, 629
MaeII ACGT 4 cut(s) 50, 127, 1064, 1325
MaeIII GTNAC 3 cut(s) 10, 59, 1060
MalI GATC 3 cut(s) 141, 936, 1080
MboI GATC 3 cut(s) 139, 934, 1078
MflI RGATCY 2 cut(s) 934, 1078
MhlI GDGCHC 1 cut(s) 415
MlsI TGGCCA 1 cut(s) 1034
MluCI AATT 4 cut(s) 198, 280, 475, 1209
MluNI TGGCCA 1 cut(s) 1034
MlyI GAGTC 3 cut(s) 920, 1064, 1346
MmeI TCCRAC 1 cut(s) 326
Mox20I TGGCCA 1 cut(s) 1034
MscI TGGCCA 1 cut(s) 1034
MseI TTAA 2 cut(s) 17, 585
MslI CAYNNNNRTG 1 cut(s) 501
Msp20I TGGCCA 1 cut(s) 1034
MspA1I CMGCKG 1 cut(s) 602
MspI CCGG 2 cut(s) 376, 888
MspR9I CCNGG 2 cut(s) 315, 717
Mva1269I GAATGC 1 cut(s) 1399
MvaI CCWGG 2 cut(s) 315, 717
MwoI GCNNNNNNNGC 9 cut(s) 261, 270, 450, 567, 576, 960, 1046, 1220, 1382
NcoI CCATGG 1 cut(s) 556
NdeII GATC 3 cut(s) 139, 934, 1078
NlaIII CATG 7 cut(s) 500, 560, 982, 1244, 1376, 1412, 1432
NlaIV GGNNCC 1 cut(s) 1110
NmuCI GTSAC 2 cut(s) 59, 1060
NspI RCATGY 3 cut(s) 500, 1376, 1412
PaeI GCATGC 1 cut(s) 1376
PagI TCATGA 1 cut(s) 1240
PceI AGGCCT 1 cut(s) 837
PciI ACATGT 1 cut(s) 496
PctI GAATGC 1 cut(s) 1399
PfeI GAWTC 3 cut(s) 338, 1153, 1289
PflFI GACNNNGTC 1 cut(s) 1092
PkrI GCNGC 8 cut(s) 266, 269, 272, 535, 601, 1277, 1371, 1384
PleI GAGTC 3 cut(s) 919, 1064, 1346
PpsI GAGTC 3 cut(s) 919, 1064, 1346
Ppu21I YACGTR 1 cut(s) 128
PscI ACATGT 1 cut(s) 496
Psp1406I AACGTT 1 cut(s) 50
Psp6I CCWGG 2 cut(s) 313, 715
PspEI GGTNACC 1 cut(s) 10
PspGI CCWGG 2 cut(s) 313, 715
PspN4I GGNNCC 1 cut(s) 1110
PspPI GGNCC 1 cut(s) 553
PstI CTGCAG 1 cut(s) 601
PstNI CAGNNNCTG 1 cut(s) 1073
PsuI RGATCY 2 cut(s) 934, 1078
PsyI GACNNNGTC 1 cut(s) 1092
PvuII CAGCTG 1 cut(s) 602
RsaI GTAC 3 cut(s) 126, 224, 1362
RsaNI GTAC 3 cut(s) 125, 223, 1361
RseI CAYNNNNRTG 1 cut(s) 501
SaqAI TTAA 2 cut(s) 17, 585
SatI GCNGC 8 cut(s) 265, 268, 271, 534, 600, 1276, 1370, 1383
Sau3AI GATC 3 cut(s) 139, 934, 1078
Sau96I GGNCC 1 cut(s) 553
ScaI AGTACT 1 cut(s) 224
SchI GAGTC 3 cut(s) 920, 1064, 1346
ScrFI CCNGG 2 cut(s) 315, 717
SduI GDGCHC 1 cut(s) 415
SfaNI GCATC 6 cut(s) 70, 732, 913, 1171, 1210, 1260
SfcI CTRYAG 2 cut(s) 597, 603
SinI GGWCC 1 cut(s) 553
SmiMI CAYNNNNRTG 1 cut(s) 501
SmlI CTYRAG 2 cut(s) 100, 1354
SmoI CTYRAG 2 cut(s) 100, 1354
SnaBI TACGTA 1 cut(s) 128
SphI GCATGC 1 cut(s) 1376
Sse9I AATT 4 cut(s) 198, 280, 475, 1209
SseBI AGGCCT 1 cut(s) 837
SsiI CCGC 2 cut(s) 264, 1276
SspMI CTAG 3 cut(s) 27, 252, 629
StuI AGGCCT 1 cut(s) 837
StyD4I CCNGG 2 cut(s) 313, 715
StyI CCWWGG 4 cut(s) 445, 556, 863, 1157
TaaI ACNGT 4 cut(s) 77, 542, 692, 1389
TaiI ACGT 4 cut(s) 53, 130, 1067, 1328
TaqI TCGA 2 cut(s) 483, 489
TaqII GACCGA 1 cut(s) 469
TasI AATT 4 cut(s) 198, 280, 475, 1209
TatI WGTACW 2 cut(s) 222, 1360
TauI GCSGC 2 cut(s) 267, 1278
TfiI GAWTC 3 cut(s) 338, 1153, 1289
Tru1I TTAA 2 cut(s) 17, 585
Tru9I TTAA 2 cut(s) 17, 585
TscAI CASTG 4 cut(s) 547, 741, 1042, 1048
TseFI GTSAC 2 cut(s) 59, 1060
TseI GCWGC 6 cut(s) 267, 270, 533, 599, 1369, 1382
Tsp45I GTSAC 2 cut(s) 59, 1060
TspDTI ATGAA 7 cut(s) 84, 207, 507, 653, 1115, 1157, 1417
TspRI CASTG 4 cut(s) 547, 741, 1042, 1048
Tth111I GACNNNGTC 1 cut(s) 1092
VpaK11BI GGWCC 1 cut(s) 553
XapI RAATTY 1 cut(s) 475
XceI RCATGY 3 cut(s) 500, 1376, 1412
XcmI CCANNNNNNNNNTGG 1 cut(s) 1177
XmiI GTMKAC 1 cut(s) 609
XspI CTAG 3 cut(s) 27, 252, 629
ZraI GACGTC 2 cut(s) 1065, 1326
ZrmI AGTACT 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.