Rh4DG380100

phytochrome kinase substrate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0013994
Physical Location & Seq
Forward (+)
59808229 .. 59811505
3277 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG380100.1

Sequence Viewer

Length: 1494 bp
ATGGCTATGGTTACCTCTAATATCCGAGACGCCTCCTTTTCTTCATACTTGAGTACTAATGAGGAAACCTTTCTATGTAAACTCGTTGATCAGAAGTCAAGCCGAAACCTCATCACAACCACCAGTCCTTCCAAGGGAGATCAGCATAAGCACTTGGGAACTAACAAGAAGGAAGAAGAGGAAGAAATTGGAGTATTTGGAGCTGAAAAATACTTCAATGGAGTAATGGACGAGGACAGCTCAAAAAGTTCAAGCAGCACCTGTAGTTCAAGGAAGTTCCATGTTCATCAGCAGCGAGTCAATCTAGATCACATGAAGCTAAAGGTACAACAGCCCGGGACTCCAAGTGTTCATTCCGAATCAAGTTGGAACAGCCAGAGTGCACTGCTTACAAGTGGTTCGAGAAATCCCAAGAGGACTCACAAGACAAGCAGCAACAAGGCACAGAGGAAAAGCTTCTTTGCAACTCTTGGTTGCTCATGTACTGATAAGCATTCTGTTGATGTTAATGAAGACCATGTTGGTGAAATCAGCTTCAAGCAGAATGGTGCTGCTGCCAACTCTGGAATCACAACACCCCACATTAAACAAAGTCTAGACCTTGTTGGTGAAGCTCTAGTCAAGATTAAACAAACCCCGGATTCATTGGTGAAGGAGGAGATATTGACGAACAACGAAAAGATGGATAAGTTGGGAACTGGATTGAGCAGAGAAAACTGCTTTACTTTCCCAGCTTCAAAATCCGGGGCCGTGAGTAACTTGCCTATCAAGATTCCTACCTTTCAAGAAATAGAAGATGAGAAACTGAGGAAGTCTATAGAGGTGTTTGGTTCTCCAGTGCTAGAAAAAAGGAAAAAGGCTTTGGGCCTTGAGAAAAGGCTAACAATGCTGTCTTGGGATGCCGAGTTTCCTGCAGCAAAATCTGGTGGAACCTACACTCATGAGTCAGACAGTGATGCAAGTTCAGACCTGTTTGAGATTGAGAGCCTCACAGGCAAGAACAACCCTTTTCTTGCAGGGCAAGCATCAGATGGTTGTGCCACCCCTACAACATGTTACGCGCCTAGTGAGGCAAGCATAGAGTGGAGTGTGGTCACTGCCAGTGCTGCAGATTTCAGCTCTGCCATGTCAGATTGTGAAGACCTTGTCAGGCCAAGTCCAACCACCACCACAAGTGCCAAAGCAGCTAGTATGAATAAAGAGATGGTGCCAATTAGGCACCGTCCAACTGTTCTGTTAGGTTGCAAGAGTCAAAAAGCTGTGAAAGTGGCGGCAAATGCGCACAGACCATACAACCAGAAGACCATTATTGACCCACAGATGATGCCTCGCAAGCCTGAAATGCCATTCATGCCATCAACAAGGTTTCAAGCTGAGACAACCTCTAAGTTGACAGGTTTTGACTCTAGAGTACAAGGGCAACATGCACTTGCACCACGGTCGCTCCCTCGTGCACATTCACCACATGCTTCTCATCTTCTGTTCATGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

497

Amino Acids

54.39

Weight (kDa)

8.57

Isoelectric Point (pI)

53.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013994)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14280 AT2G02950
fragaria_vesca FvH4_4g31450
malus_domestica MD16G1073600.v1.1
prunus_persica Prupe.1G273300_v2.0.a1
pyrus_communis pycom13g06530
rosa_chinensis RchiOBHm_Chr4g0439931
rosa_laevigata RLG00000006224
rosa_multiflora Rmu_sc0006000.1_g000001 Rmu_sc0022459.1_g000001
rosa_roxburghii Rroxscaffold_5G00380880
rosa_rugosa Rorug04G0322600
rosa_samantha Rh4AG373500 Rh4BG385800 Rh4CG400300 Rh4DG380100
rosa_wichuraiana Rw4G031940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1281
AccB1I GGYRCC 2 cut(s) 1207, 1218
AccII CGCG 1 cut(s) 1061
AciI CCGC 1 cut(s) 1271
AcyI GRCGYC 1 cut(s) 30
AfaI GTAC 4 cut(s) 55, 327, 484, 1413
AfiI CCNNNNNNNGG 1 cut(s) 134
AflIII ACRYGT 1 cut(s) 1052
AgsI TTSAA 7 cut(s) 217, 252, 270, 538, 738, 785, 1370
AjuI GAANNNNNNNTTGG 6 cut(s) 337, 369, 504, 536, 845, 877
Alw21I GWGCWC 2 cut(s) 385, 1456
Alw26I GTCTC 2 cut(s) 21, 1370
Alw44I GTGCAC 2 cut(s) 381, 1452
AlwNI CAGNNNCTG 1 cut(s) 261
Ama87I CYCGRG 1 cut(s) 335
AoxI GGCC 3 cut(s) 747, 865, 1151
ApaLI GTGCAC 2 cut(s) 381, 1452
ApeKI GCWGC 8 cut(s) 255, 292, 432, 551, 554, 914, 1106, 1184
Asp700I GAANNNNTTC 2 cut(s) 69, 455
AspLEI GCGC 2 cut(s) 1063, 1282
AspS9I GGNCC 2 cut(s) 747, 865
AsuC2I CCSGG 4 cut(s) 336, 337, 638, 745
AsuHPI GGTGA 4 cut(s) 536, 620, 661, 1452
AvaI CYCGRG 1 cut(s) 335
BaeGI GKGCMC 2 cut(s) 385, 1456
BanI GGYRCC 2 cut(s) 1207, 1218
BauI CACGAG 1 cut(s) 1449
BbsI GAAGAC 3 cut(s) 519, 1146, 1307
Bbv12I GWGCWC 2 cut(s) 385, 1456
BbvI GCAGC 8 cut(s) 267, 304, 444, 538, 541, 926, 1093, 1196
BccI CCATC 4 cut(s) 676, 1025, 1198, 1363
BceAI ACGGC 1 cut(s) 734
BcgI CGANNNNNNTGC 4 cut(s) 893, 927, 1422, 1456
BclI TGATCA 1 cut(s) 88
BcnI CCSGG 4 cut(s) 336, 337, 638, 745
BcoDI GTCTC 2 cut(s) 21, 1370
BfaI CTAG 7 cut(s) 305, 596, 617, 842, 1065, 1188, 1407
BfmI CTRYAG 4 cut(s) 262, 816, 912, 1107
BglI GCCNNNNNGGC 2 cut(s) 993, 1216
BisI GCNGC 9 cut(s) 256, 293, 433, 552, 555, 915, 1107, 1185, 1272
BlsI GCNGC 9 cut(s) 257, 294, 434, 553, 556, 916, 1108, 1186, 1273
BmcAI AGTACT 1 cut(s) 55
Bme1390I CCNGG 4 cut(s) 336, 337, 638, 745
BmeT110I CYCGRG 1 cut(s) 335
BmgT120I GGNCC 2 cut(s) 747, 865
BmiI GGNNCC 4 cut(s) 748, 931, 1209, 1220
BmrFI CCNGG 4 cut(s) 336, 337, 638, 745
BmsI GCATC 4 cut(s) 889, 946, 1034, 1314
BpiI GAAGAC 3 cut(s) 519, 1146, 1307
BplI GAGNNNNNCTC 4 cut(s) 224, 256, 1367, 1399
BpmI CTGGAG 1 cut(s) 819
BpuEI CTTGAG 2 cut(s) 70, 890
BpuMI CCSGG 4 cut(s) 336, 337, 638, 745
BsaHI GRCGYC 1 cut(s) 30
BsaJI CCNNGG 5 cut(s) 132, 335, 636, 744, 1436
BsaXI ACNNNNNCTCC 2 cut(s) 1428, 1458
Bsc4I CCNNNNNNNGG 1 cut(s) 134
Bse1I ACTGG 4 cut(s) 123, 703, 836, 1101
BseDI CCNNGG 5 cut(s) 132, 335, 636, 744, 1436
BseGI GGATG 1 cut(s) 904
BseLI CCNNNNNNNGG 1 cut(s) 134
BseMII CTCAG 2 cut(s) 797, 1365
BseNI ACTGG 4 cut(s) 123, 703, 836, 1101
BseRI GAGGAG 1 cut(s) 671
BseSI GKGCMC 2 cut(s) 385, 1456
BseXI GCAGC 8 cut(s) 267, 304, 444, 538, 541, 926, 1093, 1196
BseYI CCCAGC 1 cut(s) 730
Bsh1236I CGCG 1 cut(s) 1061
Bsh1285I CGRYCG 1 cut(s) 1442
BshFI GGCC 3 cut(s) 749, 867, 1153
BshNI GGYRCC 2 cut(s) 1207, 1218
BsiEI CGRYCG 1 cut(s) 1442
BsiHKAI GWGCWC 2 cut(s) 385, 1456
BsiHKCI CYCGRG 1 cut(s) 335
BsiSI CCGG 3 cut(s) 336, 638, 744
BslFI GGGAC 1 cut(s) 352
BslI CCNNNNNNNGG 1 cut(s) 134
BsmAI GTCTC 2 cut(s) 21, 1370
BsmBI CGTCTC 1 cut(s) 21
BsmFI GGGAC 1 cut(s) 352
BsmI GAATGC 1 cut(s) 493
BsnI GGCC 3 cut(s) 749, 867, 1153
BsoBI CYCGRG 1 cut(s) 335
Bsp1286I GDGCHC 2 cut(s) 385, 1456
Bsp143I GATC 3 cut(s) 88, 139, 307
BspACI CCGC 1 cut(s) 1271
BspANI GGCC 3 cut(s) 749, 867, 1153
BspCNI CTCAG 2 cut(s) 798, 1366
BspFNI CGCG 1 cut(s) 1061
BspHI TCATGA 1 cut(s) 940
BspLI GGNNCC 4 cut(s) 748, 931, 1209, 1220
BspMAI CTGCAG 2 cut(s) 916, 1111
BspT107I GGYRCC 2 cut(s) 1207, 1218
BsrI ACTGG 4 cut(s) 123, 703, 836, 1101
BssECI CCNNGG 5 cut(s) 132, 335, 636, 744, 1436
BssMI GATC 3 cut(s) 88, 139, 307
BssNI GRCGYC 1 cut(s) 30
BssSI CACGAG 1 cut(s) 1449
BssT1I CCWWGG 1 cut(s) 132
Bst2BI CACGAG 1 cut(s) 1449
Bst4CI ACNGT 4 cut(s) 953, 1223, 1231, 1440
Bst6I CTCTTC 1 cut(s) 171
BstACI GRCGYC 1 cut(s) 30
BstC8I GCNNGC 3 cut(s) 1023, 1075, 1334
BstDEI CTNAG 3 cut(s) 806, 1374, 1386
BstDSI CCRYGG 1 cut(s) 1436
BstEII GGTNACC 1 cut(s) 10
BstF5I GGATG 1 cut(s) 904
BstFNI CGCG 1 cut(s) 1061
BstHHI GCGC 2 cut(s) 1063, 1282
BstKTI GATC 3 cut(s) 91, 142, 310
BstMAI GTCTC 2 cut(s) 21, 1370
BstMBI GATC 3 cut(s) 88, 139, 307
BstMCI CGRYCG 1 cut(s) 1442
BstNSI RCATGY 3 cut(s) 1056, 1427, 1469
BstPI GGTNACC 1 cut(s) 10
BstSCI CCNGG 4 cut(s) 334, 335, 636, 743
BstSFI CTRYAG 4 cut(s) 262, 816, 912, 1107
BstSLI GKGCMC 2 cut(s) 385, 1456
BstUI CGCG 1 cut(s) 1061
BstV1I GCAGC 8 cut(s) 267, 304, 444, 538, 541, 926, 1093, 1196
BstV2I GAAGAC 3 cut(s) 519, 1146, 1307
BsuRI GGCC 3 cut(s) 749, 867, 1153
BtgI CCRYGG 1 cut(s) 1436
BtsCI GGATG 1 cut(s) 904
BtsI GCAGTG 2 cut(s) 383, 1095
BtsIMutI CAGTG 5 cut(s) 383, 843, 958, 1095, 1108
Cac8I GCNNGC 3 cut(s) 1023, 1075, 1334
CaiI CAGNNNCTG 1 cut(s) 261
CciI TCATGA 1 cut(s) 940
CfoI GCGC 2 cut(s) 1063, 1282
Cfr13I GGNCC 2 cut(s) 747, 865
Cfr9I CCCGGG 1 cut(s) 335
CseI GACGC 1 cut(s) 38
Csp6I GTAC 4 cut(s) 54, 326, 483, 1412
CviQI GTAC 4 cut(s) 54, 326, 483, 1412
DdeI CTNAG 3 cut(s) 806, 1374, 1386
DpnI GATC 3 cut(s) 90, 141, 309
DpnII GATC 3 cut(s) 88, 139, 307
Eam1104I CTCTTC 1 cut(s) 171
EarI CTCTTC 1 cut(s) 171
Eco130I CCWWGG 1 cut(s) 132
Eco88I CYCGRG 1 cut(s) 335
Eco91I GGTNACC 1 cut(s) 10
EcoO65I GGTNACC 1 cut(s) 10
EcoT14I CCWWGG 1 cut(s) 132
ErhI CCWWGG 1 cut(s) 132
Esp3I CGTCTC 1 cut(s) 21
FaqI GGGAC 1 cut(s) 352
FbaI TGATCA 1 cut(s) 88
Fnu4HI GCNGC 9 cut(s) 256, 293, 433, 552, 555, 915, 1107, 1185, 1272
FokI GGATG 1 cut(s) 911
Fsp4HI GCNGC 9 cut(s) 256, 293, 433, 552, 555, 915, 1107, 1185, 1272
FspAI RTGCGCAY 1 cut(s) 1281
FspBI CTAG 7 cut(s) 305, 596, 617, 842, 1065, 1188, 1407
FspI TGCGCA 1 cut(s) 1281
GlaI GCGC 2 cut(s) 1062, 1281
GluI GCNGC 9 cut(s) 256, 293, 433, 552, 555, 915, 1107, 1185, 1272
GsaI CCCAGC 1 cut(s) 734
GsuI CTGGAG 1 cut(s) 819
HaeIII GGCC 3 cut(s) 749, 867, 1153
HapII CCGG 3 cut(s) 336, 638, 744
HgaI GACGC 1 cut(s) 38
HhaI GCGC 2 cut(s) 1063, 1282
Hin1I GRCGYC 1 cut(s) 30
Hin6I GCGC 2 cut(s) 1061, 1280
HinP1I GCGC 2 cut(s) 1061, 1280
HincII GTYRAC 1 cut(s) 1392
HindII GTYRAC 1 cut(s) 1392
HindIII AAGCTT 1 cut(s) 454
HpaII CCGG 3 cut(s) 336, 638, 744
HphI GGTGA 4 cut(s) 536, 620, 661, 1452
Hpy166II GTNNAC 4 cut(s) 80, 383, 1392, 1454
Hpy188I TCNGA 7 cut(s) 26, 93, 358, 949, 967, 1030, 1132
Hpy188III TCNNGA 9 cut(s) 305, 402, 564, 596, 622, 769, 785, 941, 1407
Hpy8I GTNNAC 4 cut(s) 80, 383, 1392, 1454
HpyAV CCTTC 3 cut(s) 138, 163, 646
HpyCH4III ACNGT 4 cut(s) 953, 1223, 1231, 1440
HpyF3I CTNAG 3 cut(s) 806, 1374, 1386
Hsp92I GRCGYC 1 cut(s) 30
HspAI GCGC 2 cut(s) 1061, 1280
Ksp22I TGATCA 1 cut(s) 88
Kzo9I GATC 3 cut(s) 88, 139, 307
LmnI GCTCC 2 cut(s) 200, 1449
Lsp1109I GCAGC 8 cut(s) 267, 304, 444, 538, 541, 926, 1093, 1196
LweI GCATC 4 cut(s) 889, 946, 1034, 1314
MaeI CTAG 7 cut(s) 305, 596, 617, 842, 1065, 1188, 1407
MaeIII GTNAC 4 cut(s) 10, 755, 1055, 1093
MalI GATC 3 cut(s) 90, 141, 309
MboI GATC 3 cut(s) 88, 139, 307
MboII GAAGA 9 cut(s) 33, 185, 188, 194, 524, 806, 1151, 1312, 1469
MhlI GDGCHC 2 cut(s) 385, 1456
MluCI AATT 2 cut(s) 186, 1212
MlyI GAGTC 6 cut(s) 306, 334, 412, 953, 1258, 1397
MmeI TCCRAC 3 cut(s) 347, 1184, 1250
MroXI GAANNNNTTC 2 cut(s) 69, 455
MseI TTAA 3 cut(s) 507, 585, 627
MslI CAYNNNNRTG 1 cut(s) 522
MspI CCGG 3 cut(s) 336, 638, 744
MspR9I CCNGG 4 cut(s) 336, 337, 638, 745
Mva1269I GAATGC 1 cut(s) 493
MvnI CGCG 1 cut(s) 1061
NciI CCSGG 4 cut(s) 336, 337, 638, 745
NdeII GATC 3 cut(s) 88, 139, 307
NlaIV GGNNCC 4 cut(s) 748, 931, 1209, 1220
NmeAIII GCCGAG 1 cut(s) 928
NmuCI GTSAC 1 cut(s) 1093
NsbI TGCGCA 1 cut(s) 1281
NspI RCATGY 3 cut(s) 1056, 1427, 1469
PagI TCATGA 1 cut(s) 940
PciI ACATGT 1 cut(s) 1052
PctI GAATGC 1 cut(s) 493
PdmI GAANNNNTTC 2 cut(s) 69, 455
PfeI GAWTC 4 cut(s) 359, 567, 641, 772
PflFI GACNNNGTC 1 cut(s) 1145
PkrI GCNGC 9 cut(s) 257, 294, 434, 553, 556, 916, 1108, 1186, 1273
PleI GAGTC 6 cut(s) 305, 334, 412, 952, 1257, 1397
PpsI GAGTC 6 cut(s) 305, 334, 412, 952, 1257, 1397
PscI ACATGT 1 cut(s) 1052
PspEI GGTNACC 1 cut(s) 10
PspFI CCCAGC 1 cut(s) 730
PspN4I GGNNCC 4 cut(s) 748, 931, 1209, 1220
PspPI GGNCC 2 cut(s) 747, 865
PstI CTGCAG 2 cut(s) 916, 1111
PstNI CAGNNNCTG 1 cut(s) 261
PsyI GACNNNGTC 1 cut(s) 1145
RsaI GTAC 4 cut(s) 55, 327, 484, 1413
RsaNI GTAC 4 cut(s) 54, 326, 483, 1412
RseI CAYNNNNRTG 1 cut(s) 522
SaqAI TTAA 3 cut(s) 507, 585, 627
SatI GCNGC 9 cut(s) 256, 293, 433, 552, 555, 915, 1107, 1185, 1272
Sau3AI GATC 3 cut(s) 88, 139, 307
Sau96I GGNCC 2 cut(s) 747, 865
ScaI AGTACT 1 cut(s) 55
SchI GAGTC 6 cut(s) 306, 334, 412, 953, 1258, 1397
ScrFI CCNGG 4 cut(s) 336, 337, 638, 745
SduI GDGCHC 2 cut(s) 385, 1456
SfaNI GCATC 4 cut(s) 889, 946, 1034, 1314
SfcI CTRYAG 4 cut(s) 262, 816, 912, 1107
SmaI CCCGGG 1 cut(s) 337
SmiMI CAYNNNNRTG 1 cut(s) 522
SmlI CTYRAG 2 cut(s) 49, 869
SmoI CTYRAG 2 cut(s) 49, 869
Sse9I AATT 2 cut(s) 186, 1212
SsiI CCGC 1 cut(s) 1271
SspMI CTAG 7 cut(s) 305, 596, 617, 842, 1065, 1188, 1407
StyD4I CCNGG 4 cut(s) 334, 335, 636, 743
StyI CCWWGG 1 cut(s) 132
TaaI ACNGT 4 cut(s) 953, 1223, 1231, 1440
TaqI TCGA 1 cut(s) 401
TasI AATT 2 cut(s) 186, 1212
TatI WGTACW 3 cut(s) 53, 482, 1411
TauI GCSGC 1 cut(s) 1274
TfiI GAWTC 4 cut(s) 359, 567, 641, 772
Tru1I TTAA 3 cut(s) 507, 585, 627
Tru9I TTAA 3 cut(s) 507, 585, 627
TscAI CASTG 5 cut(s) 390, 843, 958, 1102, 1108
TseFI GTSAC 1 cut(s) 1093
TseI GCWGC 8 cut(s) 255, 292, 432, 551, 554, 914, 1106, 1184
Tsp45I GTSAC 1 cut(s) 1093
TspDTI ATGAA 9 cut(s) 33, 275, 329, 341, 525, 633, 1208, 1339, 1474
TspMI CCCGGG 1 cut(s) 335
TspRI CASTG 5 cut(s) 390, 843, 958, 1102, 1108
Tth111I GACNNNGTC 1 cut(s) 1145
VneI GTGCAC 2 cut(s) 381, 1452
XbaI TCTAGA 3 cut(s) 304, 595, 1406
XceI RCATGY 3 cut(s) 1056, 1427, 1469
XmaI CCCGGG 1 cut(s) 335
XmnI GAANNNNTTC 2 cut(s) 69, 455
XspI CTAG 7 cut(s) 305, 596, 617, 842, 1065, 1188, 1407
ZrmI AGTACT 1 cut(s) 55
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.