Rw4G031940

phytochrome kinase substrate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0013994
Physical Location & Seq
Forward (+)
59127974 .. 59130091
2118 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G031940.1

Sequence Viewer

Length: 1503 bp
ATGGCTATGGTTACCTCTAATATCCGAGACGCCTCCTTTTCTTCATACTTGAGTACTAATGAGGAAGCCTTTCTATGTAAACTCGTTGATCAGAAGTCAAGCCGAAACCTCAGTCCAGTCATCACAACCACCAGTCCTTCCAAGGGAGATCAGCATAAGCACTTGGGAACTAAAAAGAAGGAAGAAGAGGAAGAAATTGGAGTATTTGGAGCTGAAAAATACTTCAATGGAGTAATGGACGAGGACAGCTCAAAAAGTTCAAGCAGCACCTGTAGTTCAAGGAAGTTCCATGTTCATCAGCAGCGAGTCAATCTAGATCACATGAAGCTAAAGGTACAACAGCCCGGGACTCCAAGTGTTCGTTCCGAATCAAGTTGGAACAGCCAGAGTGCACTGCTTACAAGTGGTTCGAGAAATCCCAAAAGGACTAACAAGACAAGCAGCAACAAGGCACAGAGGAAAAGCTTCTTTGCAACTCTTGGTTGCTCATGTACTGATAAGCATTCTGTTGATGTTAATGAAGACCATGTTGGTGAAATCAGCTTCAAGCAGAATGGTGCTGCTGCCAACTCTGGAATCACAACACCCCACATTAAACAAAGTCTAGACCTTGTTGGTGAAGCTCTAGTCAAGATTAAACAAACCCCGGATTCATGGGTGAAGGAGGAGATATTGACAAACAACGAAAAGATGGATAAGTTGGGAACTGGATTGAGCAGAGAAAACTGCTTTACTTTCCCAGATACAAAATCCGGGGCCGTGAGTAACTTGCCTATCAAGATTCCTACCTTTCAAGAAATAGAAGATGAGAAACTGAGGAAGTCTATAGAGGTGTTTGGTTCTCCAGTGTTAGAAAAAAGGAAAAAGGCTTTAGGCCTTGAGAAAAGGCTAACAATGCTGTCTTGGGATGCCGAGTTTCCTGCAGCAAAATCTGGTGGAACCTACACTCATGAGTCAGACAGTGATGCAAGTTCAGACCTGTTTGAGATTGAGAGCCTCACAGGCAAGAACAACCCTTTTCTTGCAAGGCAAGCATCAGATGGTTGTGCCACCCCTACAACATGTTACGCGCCTAGTGAGGCAAGCATAGAGTGGAGTGTGGTCACTGCCAGTGCTGCAGATTTCAGCTCTGCCATGTCAGATTGTGAAGACCTTGTCAGGCCAAGTCCAACCACCACCACAAGTGCCAAAGCAGCTAGTATGAACAAGGAGATGGTGCCAATTAGGCACCGTCCAACTGTTCTGTTAGGTTGCAAGAGTCAAAAAGCTGTGAAAGTGGCGGCAAATGCGCACAGACCATACAACCAGAAGACCATTATTGACCCACAGATGATGCCTCGCAAGCCGGAAATGCCATTCATGCCATCAACAAGGTTTCAAGCTGAGACAACCTCTAAGTTGACAGGTTTTGACTCCAGAGTACAAGGGCAACATGCACTTGCACCACGGTCGCTCCCTCGTGCACATTCACCACATGCTTCTCATCTTCTGTTCATGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

500

Amino Acids

54.89

Weight (kDa)

8.78

Isoelectric Point (pI)

54.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013994)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14280 AT2G02950
fragaria_vesca FvH4_4g31450
malus_domestica MD16G1073600.v1.1
prunus_persica Prupe.1G273300_v2.0.a1
pyrus_communis pycom13g06530
rosa_chinensis RchiOBHm_Chr4g0439931
rosa_laevigata RLG00000006224
rosa_multiflora Rmu_sc0006000.1_g000001 Rmu_sc0022459.1_g000001
rosa_roxburghii Rroxscaffold_5G00380880
rosa_rugosa Rorug04G0322600
rosa_samantha Rh4AG373500 Rh4BG385800 Rh4CG400300 Rh4DG380100
rosa_wichuraiana Rw4G031940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1290
AccB1I GGYRCC 2 cut(s) 1216, 1227
AccII CGCG 1 cut(s) 1070
AciI CCGC 1 cut(s) 1280
AcyI GRCGYC 1 cut(s) 30
AfaI GTAC 4 cut(s) 55, 336, 493, 1422
AfiI CCNNNNNNNGG 1 cut(s) 143
AflIII ACRYGT 1 cut(s) 1061
AgsI TTSAA 6 cut(s) 226, 261, 279, 547, 794, 1379
AjuI GAANNNNNNNTTGG 4 cut(s) 346, 378, 513, 545
Alw21I GWGCWC 2 cut(s) 394, 1465
Alw26I GTCTC 2 cut(s) 21, 1379
Alw44I GTGCAC 2 cut(s) 390, 1461
AlwNI CAGNNNCTG 1 cut(s) 270
Ama87I CYCGRG 1 cut(s) 344
AoxI GGCC 3 cut(s) 756, 874, 1160
ApaLI GTGCAC 2 cut(s) 390, 1461
ApeKI GCWGC 8 cut(s) 264, 301, 441, 560, 563, 923, 1115, 1193
ArsI GACNNNNNNTTYG 2 cut(s) 97, 129
Asp700I GAANNNNTTC 2 cut(s) 69, 464
AspLEI GCGC 2 cut(s) 1072, 1291
AspS9I GGNCC 1 cut(s) 756
AsuC2I CCSGG 4 cut(s) 345, 346, 647, 754
AsuHPI GGTGA 4 cut(s) 545, 629, 670, 1461
AvaI CYCGRG 1 cut(s) 344
BaeGI GKGCMC 2 cut(s) 394, 1465
BanI GGYRCC 2 cut(s) 1216, 1227
BauI CACGAG 1 cut(s) 1458
BbsI GAAGAC 3 cut(s) 528, 1155, 1316
Bbv12I GWGCWC 2 cut(s) 394, 1465
BbvI GCAGC 8 cut(s) 276, 313, 453, 547, 550, 935, 1102, 1205
BccI CCATC 4 cut(s) 685, 1034, 1207, 1372
BceAI ACGGC 1 cut(s) 743
BcgI CGANNNNNNTGC 4 cut(s) 902, 936, 1431, 1465
BclI TGATCA 1 cut(s) 88
BcnI CCSGG 4 cut(s) 345, 346, 647, 754
BcoDI GTCTC 2 cut(s) 21, 1379
BfaI CTAG 5 cut(s) 314, 605, 626, 1074, 1197
BfmI CTRYAG 4 cut(s) 271, 825, 921, 1116
BglI GCCNNNNNGGC 2 cut(s) 1002, 1225
BisI GCNGC 9 cut(s) 265, 302, 442, 561, 564, 924, 1116, 1194, 1281
BlsI GCNGC 9 cut(s) 266, 303, 443, 562, 565, 925, 1117, 1195, 1282
BmcAI AGTACT 1 cut(s) 55
Bme1390I CCNGG 4 cut(s) 345, 346, 647, 754
BmeT110I CYCGRG 1 cut(s) 344
BmgT120I GGNCC 1 cut(s) 756
BmiI GGNNCC 4 cut(s) 757, 940, 1218, 1229
BmrFI CCNGG 4 cut(s) 345, 346, 647, 754
BmsI GCATC 4 cut(s) 898, 955, 1043, 1323
BpiI GAAGAC 3 cut(s) 528, 1155, 1316
BplI GAGNNNNNCTC 4 cut(s) 233, 265, 1376, 1408
BpmI CTGGAG 2 cut(s) 828, 1399
BpuEI CTTGAG 2 cut(s) 70, 899
BpuMI CCSGG 4 cut(s) 345, 346, 647, 754
BsaHI GRCGYC 1 cut(s) 30
BsaJI CCNNGG 5 cut(s) 141, 344, 645, 753, 1445
BsaXI ACNNNNNCTCC 2 cut(s) 1437, 1467
Bsc4I CCNNNNNNNGG 1 cut(s) 143
Bse1I ACTGG 5 cut(s) 116, 132, 712, 845, 1110
BseDI CCNNGG 5 cut(s) 141, 344, 645, 753, 1445
BseGI GGATG 1 cut(s) 913
BseLI CCNNNNNNNGG 1 cut(s) 143
BseMII CTCAG 3 cut(s) 124, 806, 1374
BseNI ACTGG 5 cut(s) 116, 132, 712, 845, 1110
BseRI GAGGAG 1 cut(s) 680
BseSI GKGCMC 2 cut(s) 394, 1465
BseXI GCAGC 8 cut(s) 276, 313, 453, 547, 550, 935, 1102, 1205
Bsh1236I CGCG 1 cut(s) 1070
Bsh1285I CGRYCG 1 cut(s) 1451
BshFI GGCC 3 cut(s) 758, 876, 1162
BshNI GGYRCC 2 cut(s) 1216, 1227
BsiEI CGRYCG 1 cut(s) 1451
BsiHKAI GWGCWC 2 cut(s) 394, 1465
BsiHKCI CYCGRG 1 cut(s) 344
BsiSI CCGG 4 cut(s) 345, 647, 753, 1346
BslFI GGGAC 1 cut(s) 361
BslI CCNNNNNNNGG 1 cut(s) 143
BsmAI GTCTC 2 cut(s) 21, 1379
BsmBI CGTCTC 1 cut(s) 21
BsmFI GGGAC 1 cut(s) 361
BsmI GAATGC 1 cut(s) 502
BsnI GGCC 3 cut(s) 758, 876, 1162
BsoBI CYCGRG 1 cut(s) 344
Bsp1286I GDGCHC 2 cut(s) 394, 1465
Bsp143I GATC 3 cut(s) 88, 148, 316
BspACI CCGC 1 cut(s) 1280
BspANI GGCC 3 cut(s) 758, 876, 1162
BspCNI CTCAG 3 cut(s) 123, 807, 1375
BspFNI CGCG 1 cut(s) 1070
BspHI TCATGA 1 cut(s) 949
BspLI GGNNCC 4 cut(s) 757, 940, 1218, 1229
BspMAI CTGCAG 2 cut(s) 925, 1120
BspT107I GGYRCC 2 cut(s) 1216, 1227
BsrI ACTGG 5 cut(s) 116, 132, 712, 845, 1110
BssECI CCNNGG 5 cut(s) 141, 344, 645, 753, 1445
BssMI GATC 3 cut(s) 88, 148, 316
BssNI GRCGYC 1 cut(s) 30
BssSI CACGAG 1 cut(s) 1458
BssT1I CCWWGG 1 cut(s) 141
Bst2BI CACGAG 1 cut(s) 1458
Bst4CI ACNGT 4 cut(s) 962, 1232, 1240, 1449
Bst6I CTCTTC 1 cut(s) 180
BstACI GRCGYC 1 cut(s) 30
BstC8I GCNNGC 3 cut(s) 1032, 1084, 1343
BstDEI CTNAG 4 cut(s) 110, 815, 1383, 1395
BstDSI CCRYGG 1 cut(s) 1445
BstEII GGTNACC 1 cut(s) 10
BstF5I GGATG 1 cut(s) 913
BstFNI CGCG 1 cut(s) 1070
BstHHI GCGC 2 cut(s) 1072, 1291
BstKTI GATC 3 cut(s) 91, 151, 319
BstMAI GTCTC 2 cut(s) 21, 1379
BstMBI GATC 3 cut(s) 88, 148, 316
BstMCI CGRYCG 1 cut(s) 1451
BstNSI RCATGY 3 cut(s) 1065, 1436, 1478
BstPI GGTNACC 1 cut(s) 10
BstSCI CCNGG 4 cut(s) 343, 344, 645, 752
BstSFI CTRYAG 4 cut(s) 271, 825, 921, 1116
BstSLI GKGCMC 2 cut(s) 394, 1465
BstUI CGCG 1 cut(s) 1070
BstV1I GCAGC 8 cut(s) 276, 313, 453, 547, 550, 935, 1102, 1205
BstV2I GAAGAC 3 cut(s) 528, 1155, 1316
BsuRI GGCC 3 cut(s) 758, 876, 1162
BtgI CCRYGG 1 cut(s) 1445
BtsCI GGATG 1 cut(s) 913
BtsI GCAGTG 2 cut(s) 392, 1104
BtsIMutI CAGTG 5 cut(s) 392, 852, 967, 1104, 1117
Cac8I GCNNGC 3 cut(s) 1032, 1084, 1343
CaiI CAGNNNCTG 1 cut(s) 270
CciI TCATGA 1 cut(s) 949
CfoI GCGC 2 cut(s) 1072, 1291
Cfr13I GGNCC 1 cut(s) 756
Cfr9I CCCGGG 1 cut(s) 344
CseI GACGC 1 cut(s) 38
Csp6I GTAC 4 cut(s) 54, 335, 492, 1421
CviQI GTAC 4 cut(s) 54, 335, 492, 1421
DdeI CTNAG 4 cut(s) 110, 815, 1383, 1395
DpnI GATC 3 cut(s) 90, 150, 318
DpnII GATC 3 cut(s) 88, 148, 316
Eam1104I CTCTTC 1 cut(s) 180
EarI CTCTTC 1 cut(s) 180
Eco130I CCWWGG 1 cut(s) 141
Eco147I AGGCCT 1 cut(s) 876
Eco88I CYCGRG 1 cut(s) 344
Eco91I GGTNACC 1 cut(s) 10
EcoO65I GGTNACC 1 cut(s) 10
EcoT14I CCWWGG 1 cut(s) 141
ErhI CCWWGG 1 cut(s) 141
Esp3I CGTCTC 1 cut(s) 21
FaqI GGGAC 1 cut(s) 361
FbaI TGATCA 1 cut(s) 88
Fnu4HI GCNGC 9 cut(s) 265, 302, 442, 561, 564, 924, 1116, 1194, 1281
FokI GGATG 1 cut(s) 920
Fsp4HI GCNGC 9 cut(s) 265, 302, 442, 561, 564, 924, 1116, 1194, 1281
FspAI RTGCGCAY 1 cut(s) 1290
FspBI CTAG 5 cut(s) 314, 605, 626, 1074, 1197
FspI TGCGCA 1 cut(s) 1290
GlaI GCGC 2 cut(s) 1071, 1290
GluI GCNGC 9 cut(s) 265, 302, 442, 561, 564, 924, 1116, 1194, 1281
GsuI CTGGAG 2 cut(s) 828, 1399
HaeIII GGCC 3 cut(s) 758, 876, 1162
HapII CCGG 4 cut(s) 345, 647, 753, 1346
HgaI GACGC 1 cut(s) 38
HhaI GCGC 2 cut(s) 1072, 1291
Hin1I GRCGYC 1 cut(s) 30
Hin6I GCGC 2 cut(s) 1070, 1289
HinP1I GCGC 2 cut(s) 1070, 1289
HincII GTYRAC 1 cut(s) 1401
HindII GTYRAC 1 cut(s) 1401
HindIII AAGCTT 1 cut(s) 463
HinfI GANTC 9 cut(s) 306, 349, 368, 576, 650, 781, 953, 1258, 1412
HpaII CCGG 4 cut(s) 345, 647, 753, 1346
HphI GGTGA 4 cut(s) 545, 629, 670, 1461
Hpy166II GTNNAC 4 cut(s) 80, 392, 1401, 1463
Hpy188I TCNGA 7 cut(s) 26, 93, 367, 958, 976, 1039, 1141
Hpy188III TCNNGA 9 cut(s) 314, 411, 573, 605, 631, 778, 794, 950, 1416
Hpy8I GTNNAC 4 cut(s) 80, 392, 1401, 1463
HpyAV CCTTC 3 cut(s) 147, 172, 655
HpyCH4III ACNGT 4 cut(s) 962, 1232, 1240, 1449
HpyF3I CTNAG 4 cut(s) 110, 815, 1383, 1395
Hsp92I GRCGYC 1 cut(s) 30
HspAI GCGC 2 cut(s) 1070, 1289
Ksp22I TGATCA 1 cut(s) 88
Kzo9I GATC 3 cut(s) 88, 148, 316
LmnI GCTCC 2 cut(s) 209, 1458
Lsp1109I GCAGC 8 cut(s) 276, 313, 453, 547, 550, 935, 1102, 1205
LweI GCATC 4 cut(s) 898, 955, 1043, 1323
MaeI CTAG 5 cut(s) 314, 605, 626, 1074, 1197
MaeIII GTNAC 4 cut(s) 10, 764, 1064, 1102
MalI GATC 3 cut(s) 90, 150, 318
MboI GATC 3 cut(s) 88, 148, 316
MboII GAAGA 9 cut(s) 33, 194, 197, 203, 533, 815, 1160, 1321, 1478
MhlI GDGCHC 2 cut(s) 394, 1465
MluCI AATT 2 cut(s) 195, 1221
MlyI GAGTC 5 cut(s) 315, 343, 962, 1267, 1406
MmeI TCCRAC 3 cut(s) 356, 1193, 1259
MroXI GAANNNNTTC 2 cut(s) 69, 464
MseI TTAA 3 cut(s) 516, 594, 636
MslI CAYNNNNRTG 1 cut(s) 531
MspI CCGG 4 cut(s) 345, 647, 753, 1346
MspR9I CCNGG 4 cut(s) 345, 346, 647, 754
Mva1269I GAATGC 1 cut(s) 502
MvnI CGCG 1 cut(s) 1070
NciI CCSGG 4 cut(s) 345, 346, 647, 754
NdeII GATC 3 cut(s) 88, 148, 316
NlaIV GGNNCC 4 cut(s) 757, 940, 1218, 1229
NmeAIII GCCGAG 1 cut(s) 937
NmuCI GTSAC 1 cut(s) 1102
NsbI TGCGCA 1 cut(s) 1290
NspI RCATGY 3 cut(s) 1065, 1436, 1478
PagI TCATGA 1 cut(s) 949
PceI AGGCCT 1 cut(s) 876
PciI ACATGT 1 cut(s) 1061
PctI GAATGC 1 cut(s) 502
PdmI GAANNNNTTC 2 cut(s) 69, 464
PfeI GAWTC 4 cut(s) 368, 576, 650, 781
PflFI GACNNNGTC 1 cut(s) 1154
PkrI GCNGC 9 cut(s) 266, 303, 443, 562, 565, 925, 1117, 1195, 1282
PleI GAGTC 5 cut(s) 314, 343, 961, 1266, 1406
PpsI GAGTC 5 cut(s) 314, 343, 961, 1266, 1406
PscI ACATGT 1 cut(s) 1061
PspEI GGTNACC 1 cut(s) 10
PspN4I GGNNCC 4 cut(s) 757, 940, 1218, 1229
PspPI GGNCC 1 cut(s) 756
PstI CTGCAG 2 cut(s) 925, 1120
PstNI CAGNNNCTG 1 cut(s) 270
PsyI GACNNNGTC 1 cut(s) 1154
RsaI GTAC 4 cut(s) 55, 336, 493, 1422
RsaNI GTAC 4 cut(s) 54, 335, 492, 1421
RseI CAYNNNNRTG 1 cut(s) 531
SaqAI TTAA 3 cut(s) 516, 594, 636
SatI GCNGC 9 cut(s) 265, 302, 442, 561, 564, 924, 1116, 1194, 1281
Sau3AI GATC 3 cut(s) 88, 148, 316
Sau96I GGNCC 1 cut(s) 756
ScaI AGTACT 1 cut(s) 55
SchI GAGTC 5 cut(s) 315, 343, 962, 1267, 1406
ScrFI CCNGG 4 cut(s) 345, 346, 647, 754
SduI GDGCHC 2 cut(s) 394, 1465
SfaNI GCATC 4 cut(s) 898, 955, 1043, 1323
SfcI CTRYAG 4 cut(s) 271, 825, 921, 1116
SmaI CCCGGG 1 cut(s) 346
SmiMI CAYNNNNRTG 1 cut(s) 531
SmlI CTYRAG 2 cut(s) 49, 878
SmoI CTYRAG 2 cut(s) 49, 878
Sse9I AATT 2 cut(s) 195, 1221
SseBI AGGCCT 1 cut(s) 876
SsiI CCGC 1 cut(s) 1280
SspMI CTAG 5 cut(s) 314, 605, 626, 1074, 1197
StuI AGGCCT 1 cut(s) 876
StyD4I CCNGG 4 cut(s) 343, 344, 645, 752
StyI CCWWGG 1 cut(s) 141
TaaI ACNGT 4 cut(s) 962, 1232, 1240, 1449
TaqI TCGA 1 cut(s) 410
TasI AATT 2 cut(s) 195, 1221
TatI WGTACW 3 cut(s) 53, 491, 1420
TauI GCSGC 1 cut(s) 1283
TfiI GAWTC 4 cut(s) 368, 576, 650, 781
Tru1I TTAA 3 cut(s) 516, 594, 636
Tru9I TTAA 3 cut(s) 516, 594, 636
TscAI CASTG 5 cut(s) 399, 852, 967, 1111, 1117
TseFI GTSAC 1 cut(s) 1102
TseI GCWGC 8 cut(s) 264, 301, 441, 560, 563, 923, 1115, 1193
Tsp45I GTSAC 1 cut(s) 1102
TspDTI ATGAA 8 cut(s) 33, 284, 338, 534, 642, 1217, 1348, 1483
TspMI CCCGGG 1 cut(s) 344
TspRI CASTG 5 cut(s) 399, 852, 967, 1111, 1117
Tth111I GACNNNGTC 1 cut(s) 1154
VneI GTGCAC 2 cut(s) 390, 1461
XbaI TCTAGA 2 cut(s) 313, 604
XceI RCATGY 3 cut(s) 1065, 1436, 1478
XmaI CCCGGG 1 cut(s) 344
XmnI GAANNNNTTC 2 cut(s) 69, 464
XspI CTAG 5 cut(s) 314, 605, 626, 1074, 1197
ZrmI AGTACT 1 cut(s) 55
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.