Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0008021

Physical Location & Seq

Genomic Coordinates Forward (+)

7450473 .. 7455032

Gene Structure 4560 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1106100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1284 bp

ATGGACCCGGCCTCTCGCCCAGCTGTAGTAATCGACAATGGAAGTGGGTATACTAAAATGGGTTTCGCGGGAAATGTCGAGCCATGTTACACTGTTCCGACTGTAGTTGCATTAAACGAATCGTTTTTGAACCCGTCCAGAACCTCCTCCAAGTCCGGCTGGATAGCGCAGCACAACGCTGGCGTGATGGCTGATCTTGATTTCTTTATTGGGGATGAGGCGCTGGAAAGATCTCGATCAAGTACAACTTACAATCTAAGCTATCCCGTTCGACAGGGCCAAGTCGAGAATTGGGATTCAATGGAGCGGTTCTGGCAGCAAAGTATATTTAACTACTTGCGTTGTGATCCTGAAGACCATTATTTTCTTTTAACCGAGAGTCCGCTTACTGCCCCGGAGAGCCGGGAGTATACCGGTGAAATCATGTTCGAAACTTTCAATGTTCCTGGCCTTTACATTGCCGTGAATTCGGTACTTGCTCTTGCAGCTGGTTACACTACCTCCAAGTGTGAGATGACAGGGGTTGTAGTAGACGTTGGAGATGGGGCAACTCATATTGTTCCTGTTGCAGAAGGTTATGTTATTGGGAGCAGCATCAAGTCAGTTCCTATTGCCGGGAAAGATGTCACTCTCTTCGTCCAGCAGCTTATGCGGGAAAGAGGAGAGAATGTGCCACCAGACGACTCCTTTGATGTAGCTCGCAGAGTGAAGGAAATGTATTGCTATACTAGTTCTGATATTGTCAAGGAGTTTAATAAGCACGATAAAGAACCATCCAAGTATATTAAGCAATGGAGAGGCATCAAACCAAAAACAGGGGCGCCCTACTCTTGTGACATTGGCTATGAACGATTTCTTGGCCCAGAGGTTTTCTTCAATCCTGAGATTTATAGCACCGACTTCACCACTCCCTTACCCGTTGTAATTGACAAGTGTATTCAGTCTGCTCCAATTGACACTAGGAGGTCTTTGTATAAGAATATAGTGTTGTCTGGAGGTTCAACCATGTTCAAGGACTTCCATAGGAGGTTGCAACGGGATATAAAGAAGATTGTGGATGCCCGGCTTCTTGCATCTGAAGCTCGAGTTGGTGGAGAAGTAAAATCACATCCAGTGGAAGTGAATGTAGTTAGCCATCCCATCCAGAGATTTGCAGTTTGGTTTGGCGGCTCAGTACTTGCATCCACACCTGAATTCTTTGCAGCTTGCCATACAAAAGAAGAGTATGAGGAATACGGTGCAAGCATATGCAGAACAAATCCTGTTTTCAAGGGGATGTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0000904 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005856 GO:0005885 GO:0006996 GO:0007010 GO:0007015 GO:0008150 GO:0009653 GO:0009825 GO:0009888 GO:0009987 GO:0010026 GO:0010090 GO:0015629 GO:0016043 GO:0016049 GO:0030029 GO:0030036 GO:0030154 GO:0032502 GO:0032989 GO:0032991 GO:0040007 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0048468 GO:0048856 GO:0048869 GO:0071840 GO:0090558 GO:0090626 GO:0097435

KEGG Pathways

Metabolic & Signaling

ko03015 ko04138 ko04530 map03015 map04138 map04530

Pfam Domains

Protein Families

Actin

Protein Analysis

428

Amino Acids

47.75

Weight (kDa)

5.52

Isoelectric Point (pI)

43.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Actin	PF00022	7 - 418	9e-80	Actin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008021)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13180
fragaria_vesca	FvH4_4g25180 FvH4_4g25180 FvH4_4g25180 FvH4_4g25180 FvH4_4g25180 FvH4_4g25180
malus_domestica	MD13G1105900.v1.1 MD16G1106100.v1.1
prunus_persica	Prupe.1G240300_v2.0.a1
pyrus_communis	pycom13g09190 pycom16g09020
rosa_chinensis	RchiOBHm_Chr4g0432381
rosa_laevigata	RLG00000006848
rosa_multiflora	Rmu_sc0001084.1_g000035
rosa_roxburghii	Rroxscaffold_5G00373830
rosa_rugosa	Rorug04G0258000 Rorug04G0258000 Rorug04G0258000
rosa_samantha	Rh4AG313900 Rh4BG321400 Rh4CG336500 Rh4DG317000
rosa_wichuraiana	Rw4G027280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	820
AccBSI	CCGCTC	1 cut(s)	307
AccI	GTMKAC	3 cut(s)	50, 410, 531
AccII	CGCG	1 cut(s)	68
AciI	CCGC	5 cut(s)	68, 307, 383, 652, 1167
AclWI	GGATC	1 cut(s)	341
AcsI	RAATTY	2 cut(s)	466, 1193
AcuI	CTGAAG	2 cut(s)	372, 1098
AcyI	GRCGYC	1 cut(s)	821
AfaI	GTAC	3 cut(s)	244, 474, 1176
AfiI	CCNNNNNNNGG	2 cut(s)	614, 815
AgeI	ACCGGT	1 cut(s)	413
AgsI	TTSAA	7 cut(s)	130, 300, 439, 877, 1002, 1012, 1270
AhlI	ACTAGT	1 cut(s)	728
AjnI	CCWGG	1 cut(s)	445
AjuI	GAANNNNNNNTTGG	4 cut(s)	840, 872, 1071, 1103
AluBI	AGCT	7 cut(s)	23, 261, 488, 646, 698, 1082, 1205
AluI	AGCT	7 cut(s)	23, 261, 488, 646, 698, 1082, 1205
AlwI	GGATC	1 cut(s)	341
Ama87I	CYCGRG	1 cut(s)	1083
AoxI	GGCC	4 cut(s)	9, 277, 448, 859
ApeKI	GCWGC	6 cut(s)	169, 316, 485, 591, 643, 1202
ApoI	RAATTY	2 cut(s)	466, 1193
ArsI	GACNNNNNNTTYG	2 cut(s)	671, 703
AsiGI	ACCGGT	1 cut(s)	413
Asp700I	GAANNNNTTC	1 cut(s)	852
AspLEI	GCGC	3 cut(s)	169, 223, 823
AspS9I	GGNCC	3 cut(s)	4, 277, 860
AsuC2I	CCSGG	5 cut(s)	8, 395, 404, 616, 1063
AsuHPI	GGTGA	2 cut(s)	428, 895
AsuII	TTCGAA	1 cut(s)	429
AvaI	CYCGRG	1 cut(s)	1083
AvaII	GGWCC	1 cut(s)	4
BanI	GGYRCC	1 cut(s)	820
BarI	GAAGNNNNNNTAC	2 cut(s)	34, 66
BbsI	GAAGAC	1 cut(s)	360
BbvI	GCAGC	6 cut(s)	181, 328, 497, 603, 655, 1214
BccI	CCATC	5 cut(s)	181, 536, 781, 1143, 1148
BceAI	ACGGC	1 cut(s)	446
BciT130I	CCWGG	1 cut(s)	447
BcnI	CCSGG	5 cut(s)	8, 395, 404, 616, 1063
BcuI	ACTAGT	1 cut(s)	728
BfaI	CTAG	2 cut(s)	729, 960
BfmI	CTRYAG	2 cut(s)	24, 102
BfoI	RGCGCY	2 cut(s)	224, 824
BglII	AGATCT	1 cut(s)	230
BisI	GCNGC	7 cut(s)	170, 317, 486, 592, 644, 1168, 1203
BlsI	GCNGC	7 cut(s)	171, 318, 487, 593, 645, 1169, 1204
BmcAI	AGTACT	1 cut(s)	1176
Bme1390I	CCNGG	6 cut(s)	8, 395, 404, 447, 616, 1063
Bme18I	GGWCC	1 cut(s)	4
BmeT110I	CYCGRG	1 cut(s)	1083
BmgT120I	GGNCC	3 cut(s)	4, 277, 860
BmiI	GGNNCC	2 cut(s)	6, 822
BmrFI	CCNGG	6 cut(s)	8, 395, 404, 447, 616, 1063
BmsI	GCATC	5 cut(s)	603, 810, 1048, 1082, 1190
BpiI	GAAGAC	1 cut(s)	360
BpmI	CTGGAG	1 cut(s)	1014
Bpu14I	TTCGAA	1 cut(s)	429
BpuMI	CCSGG	5 cut(s)	8, 395, 404, 616, 1063
BsaBI	GATNNNNATC	1 cut(s)	235
BsaHI	GRCGYC	1 cut(s)	821
BsaJI	CCNNGG	1 cut(s)	393
BsaWI	WCCGGW	1 cut(s)	413
BsaXI	ACNNNNNCTCC	4 cut(s)	485, 515, 654, 684
Bsc4I	CCNNNNNNNGG	2 cut(s)	614, 815
Bse118I	RCCGGY	1 cut(s)	413
Bse1I	ACTGG	1 cut(s)	1112
Bse3DI	GCAATG	2 cut(s)	456, 797
Bse8I	GATNNNNATC	1 cut(s)	235
BseBI	CCWGG	1 cut(s)	447
BseDI	CCNNGG	1 cut(s)	393
BseGI	GGATG	8 cut(s)	220, 773, 1063, 1108, 1135, 1140, 1181, 1281
BseJI	GATNNNNATC	1 cut(s)	235
BseLI	CCNNNNNNNGG	2 cut(s)	614, 815
BseMI	GCAATG	2 cut(s)	456, 797
BseMII	CTCAG	2 cut(s)	873, 1185
BseNI	ACTGG	1 cut(s)	1112
BseRI	GAGGAG	2 cut(s)	136, 675
BseXI	GCAGC	6 cut(s)	181, 328, 497, 603, 655, 1214
BseYI	CCCAGC	1 cut(s)	19
Bsh1236I	CGCG	1 cut(s)	68
BshFI	GGCC	4 cut(s)	11, 279, 450, 861
BshNI	GGYRCC	1 cut(s)	820
BshTI	ACCGGT	1 cut(s)	413
BsiHKCI	CYCGRG	1 cut(s)	1083
BsiSI	CCGG	7 cut(s)	8, 156, 395, 403, 414, 615, 1063
BslI	CCNNNNNNNGG	2 cut(s)	614, 815
BsnI	GGCC	4 cut(s)	11, 279, 450, 861
BsoBI	CYCGRG	1 cut(s)	1083
Bsp119I	TTCGAA	1 cut(s)	429
Bsp143I	GATC	4 cut(s)	193, 230, 236, 346
BspACI	CCGC	5 cut(s)	68, 307, 383, 652, 1167
BspANI	GGCC	4 cut(s)	11, 279, 450, 861
BspCNI	CTCAG	2 cut(s)	874, 1184
BspFNI	CGCG	1 cut(s)	68
BspLI	GGNNCC	2 cut(s)	6, 822
BspPI	GGATC	1 cut(s)	341
BspT104I	TTCGAA	1 cut(s)	429
BspT107I	GGYRCC	1 cut(s)	820
BsrBI	CCGCTC	1 cut(s)	307
BsrDI	GCAATG	2 cut(s)	456, 797
BsrFI	RCCGGY	1 cut(s)	413
BsrI	ACTGG	1 cut(s)	1112
BssAI	RCCGGY	1 cut(s)	413
BssECI	CCNNGG	1 cut(s)	393
BssMI	GATC	4 cut(s)	193, 230, 236, 346
BssNAI	GTATAC	2 cut(s)	51, 411
BssNI	GRCGYC	1 cut(s)	821
Bst1107I	GTATAC	2 cut(s)	51, 411
Bst2UI	CCWGG	1 cut(s)	447
Bst4CI	ACNGT	3 cut(s)	94, 103, 1238
Bst6I	CTCTTC	2 cut(s)	638, 1215
BstACI	GRCGYC	1 cut(s)	821
BstAPI	GCANNNNNTGC	1 cut(s)	649
BstBI	TTCGAA	1 cut(s)	429
BstC8I	GCNNGC	4 cut(s)	181, 700, 1207, 1243
BstDEI	CTNAG	3 cut(s)	257, 882, 1171
BstF5I	GGATG	8 cut(s)	220, 773, 1063, 1108, 1135, 1140, 1181, 1281
BstFNI	CGCG	1 cut(s)	68
BstH2I	RGCGCY	2 cut(s)	224, 824
BstHHI	GCGC	3 cut(s)	169, 223, 823
BstKTI	GATC	4 cut(s)	196, 233, 239, 349
BstMBI	GATC	4 cut(s)	193, 230, 236, 346
BstMWI	GCNNNNNNNGC	4 cut(s)	313, 485, 649, 1079
BstNI	CCWGG	1 cut(s)	447
BstSCI	CCNGG	6 cut(s)	6, 393, 402, 445, 614, 1061
BstSFI	CTRYAG	2 cut(s)	24, 102
BstUI	CGCG	1 cut(s)	68
BstV1I	GCAGC	6 cut(s)	181, 328, 497, 603, 655, 1214
BstV2I	GAAGAC	1 cut(s)	360
BstX2I	RGATCY	1 cut(s)	230
BstYI	RGATCY	1 cut(s)	230
BstZ17I	GTATAC	2 cut(s)	51, 411
BsuRI	GGCC	4 cut(s)	11, 279, 450, 861
BtsCI	GGATG	8 cut(s)	220, 773, 1063, 1108, 1135, 1140, 1181, 1281
BtsIMutI	CAGTG	2 cut(s)	90, 1119
Cac8I	GCNNGC	4 cut(s)	181, 700, 1207, 1243
CfoI	GCGC	3 cut(s)	169, 223, 823
Cfr10I	RCCGGY	1 cut(s)	413
Cfr13I	GGNCC	3 cut(s)	4, 277, 860
Csp6I	GTAC	3 cut(s)	243, 473, 1175
CspAI	ACCGGT	1 cut(s)	413
CspCI	CAANNNNNGTGG	2 cut(s)	25, 60
CviAII	CATG	3 cut(s)	84, 424, 1006
CviQI	GTAC	3 cut(s)	243, 473, 1175
DdeI	CTNAG	3 cut(s)	257, 882, 1171
DinI	GGCGCC	1 cut(s)	822
DpnI	GATC	4 cut(s)	195, 232, 238, 348
DpnII	GATC	4 cut(s)	193, 230, 236, 346
Eam1104I	CTCTTC	2 cut(s)	638, 1215
EarI	CTCTTC	2 cut(s)	638, 1215
Eco47I	GGWCC	1 cut(s)	4
Eco57I	CTGAAG	2 cut(s)	372, 1098
Eco88I	CYCGRG	1 cut(s)	1083
EcoRI	GAATTC	2 cut(s)	466, 1193
EcoRII	CCWGG	1 cut(s)	445
EgeI	GGCGCC	1 cut(s)	822
EheI	GGCGCC	1 cut(s)	822
FaeI	CATG	3 cut(s)	87, 427, 1009
FalI	AAGNNNNNCTT	2 cut(s)	232, 264
FatI	CATG	3 cut(s)	83, 423, 1005
FauI	CCCGC	2 cut(s)	61, 645
FauNDI	CATATG	1 cut(s)	1247
FblI	GTMKAC	3 cut(s)	50, 410, 531
Fnu4HI	GCNGC	7 cut(s)	170, 317, 486, 592, 644, 1168, 1203
FokI	GGATG	7 cut(s)	227, 760, 1070, 1095, 1122, 1127, 1168
Fsp4HI	GCNGC	7 cut(s)	170, 317, 486, 592, 644, 1168, 1203
FspBI	CTAG	2 cut(s)	729, 960
GlaI	GCGC	3 cut(s)	168, 222, 822
GluI	GCNGC	7 cut(s)	170, 317, 486, 592, 644, 1168, 1203
GsaI	CCCAGC	1 cut(s)	23
GsuI	CTGGAG	1 cut(s)	1014
HaeII	RGCGCY	2 cut(s)	224, 824
HaeIII	GGCC	4 cut(s)	11, 279, 450, 861
HapII	CCGG	7 cut(s)	8, 156, 395, 403, 414, 615, 1063
HhaI	GCGC	3 cut(s)	169, 223, 823
Hin1I	GRCGYC	1 cut(s)	821
Hin1II	CATG	3 cut(s)	87, 427, 1009
Hin6I	GCGC	3 cut(s)	167, 221, 821
HinP1I	GCGC	3 cut(s)	167, 221, 821
HinfI	GANTC	4 cut(s)	119, 296, 379, 683
HpaII	CCGG	7 cut(s)	8, 156, 395, 403, 414, 615, 1063
HphI	GGTGA	2 cut(s)	428, 895
Hpy166II	GTNNAC	3 cut(s)	51, 411, 532
Hpy188I	TCNGA	3 cut(s)	99, 736, 1078
Hpy188III	TCNNGA	8 cut(s)	138, 197, 234, 286, 350, 881, 993, 1144
Hpy8I	GTNNAC	3 cut(s)	51, 411, 532
HpyAV	CCTTC	2 cut(s)	566, 703
HpyCH4III	ACNGT	3 cut(s)	94, 103, 1238
HpyCH4IV	ACGT	1 cut(s)	534
HpyF10VI	GCNNNNNNNGC	4 cut(s)	313, 485, 649, 1079
HpyF3I	CTNAG	3 cut(s)	257, 882, 1171
HpySE526I	ACGT	1 cut(s)	534
Hsp92I	GRCGYC	1 cut(s)	821
Hsp92II	CATG	3 cut(s)	87, 427, 1009
HspAI	GCGC	3 cut(s)	167, 221, 821
KasI	GGCGCC	1 cut(s)	820
Kzo9I	GATC	4 cut(s)	193, 230, 236, 346
LmnI	GCTCC	3 cut(s)	304, 588, 952
Lsp1109I	GCAGC	6 cut(s)	181, 328, 497, 603, 655, 1214
LweI	GCATC	5 cut(s)	603, 810, 1048, 1082, 1190
MaeI	CTAG	2 cut(s)	729, 960
MaeII	ACGT	1 cut(s)	534
MaeIII	GTNAC	4 cut(s)	86, 491, 625, 833
MalI	GATC	4 cut(s)	195, 232, 238, 348
MbiI	CCGCTC	1 cut(s)	307
MboI	GATC	4 cut(s)	193, 230, 236, 346
MboII	GAAGA	5 cut(s)	365, 625, 865, 1060, 1232
MfeI	CAATTG	1 cut(s)	951
MflI	RGATCY	1 cut(s)	230
MluCI	AATT	5 cut(s)	289, 466, 924, 951, 1193
Mly113I	GGCGCC	1 cut(s)	821
MlyI	GAGTC	2 cut(s)	388, 677
MmeI	TCCRAC	2 cut(s)	122, 517
MroXI	GAANNNNTTC	1 cut(s)	852
MseI	TTAA	5 cut(s)	113, 330, 371, 753, 786
MslI	CAYNNNNRTG	1 cut(s)	461
MspA1I	CMGCKG	2 cut(s)	23, 488
MspI	CCGG	7 cut(s)	8, 156, 395, 403, 414, 615, 1063
MspR9I	CCNGG	6 cut(s)	8, 395, 404, 447, 616, 1063
MunI	CAATTG	1 cut(s)	951
MvaI	CCWGG	1 cut(s)	447
MvnI	CGCG	1 cut(s)	68
MwoI	GCNNNNNNNGC	4 cut(s)	313, 485, 649, 1079
NarI	GGCGCC	1 cut(s)	821
NciI	CCSGG	5 cut(s)	8, 395, 404, 616, 1063
NdeI	CATATG	1 cut(s)	1247
NdeII	GATC	4 cut(s)	193, 230, 236, 346
NlaIII	CATG	3 cut(s)	87, 427, 1009
NlaIV	GGNNCC	2 cut(s)	6, 822
NmuCI	GTSAC	2 cut(s)	625, 833
NspV	TTCGAA	1 cut(s)	429
PaeR7I	CTCGAG	1 cut(s)	1083
PdmI	GAANNNNTTC	1 cut(s)	852
PfeI	GAWTC	2 cut(s)	119, 296
PinAI	ACCGGT	1 cut(s)	413
PkrI	GCNGC	7 cut(s)	171, 318, 487, 593, 645, 1169, 1204
PleI	GAGTC	2 cut(s)	387, 677
PluTI	GGCGCC	1 cut(s)	824
PpsI	GAGTC	2 cut(s)	387, 677
Psp6I	CCWGG	1 cut(s)	445
PspFI	CCCAGC	1 cut(s)	19
PspGI	CCWGG	1 cut(s)	445
PspN4I	GGNNCC	2 cut(s)	6, 822
PspPI	GGNCC	3 cut(s)	4, 277, 860
PspXI	VCTCGAGB	1 cut(s)	1083
PsuI	RGATCY	1 cut(s)	230
PvuII	CAGCTG	2 cut(s)	23, 488
RsaI	GTAC	3 cut(s)	244, 474, 1176
RsaNI	GTAC	3 cut(s)	243, 473, 1175
RseI	CAYNNNNRTG	1 cut(s)	461
SaqAI	TTAA	5 cut(s)	113, 330, 371, 753, 786
SatI	GCNGC	7 cut(s)	170, 317, 486, 592, 644, 1168, 1203
Sau3AI	GATC	4 cut(s)	193, 230, 236, 346
Sau96I	GGNCC	3 cut(s)	4, 277, 860
ScaI	AGTACT	1 cut(s)	1176
SchI	GAGTC	2 cut(s)	388, 677
ScrFI	CCNGG	6 cut(s)	8, 395, 404, 447, 616, 1063
SfaNI	GCATC	5 cut(s)	603, 810, 1048, 1082, 1190
SfcI	CTRYAG	2 cut(s)	24, 102
SfoI	GGCGCC	1 cut(s)	822
Sfr274I	CTCGAG	1 cut(s)	1083
SfuI	TTCGAA	1 cut(s)	429
SinI	GGWCC	1 cut(s)	4
SlaI	CTCGAG	1 cut(s)	1083
SmiMI	CAYNNNNRTG	1 cut(s)	461
SmlI	CTYRAG	1 cut(s)	1083
SmoI	CTYRAG	1 cut(s)	1083
SpeI	ACTAGT	1 cut(s)	728
Sse9I	AATT	5 cut(s)	289, 466, 924, 951, 1193
SsiI	CCGC	5 cut(s)	68, 307, 383, 652, 1167
SspDI	GGCGCC	1 cut(s)	820
SspMI	CTAG	2 cut(s)	729, 960
StyD4I	CCNGG	6 cut(s)	6, 393, 402, 445, 614, 1061
TaaI	ACNGT	3 cut(s)	94, 103, 1238
TaiI	ACGT	1 cut(s)	537
TaqI	TCGA	7 cut(s)	33, 78, 235, 271, 285, 429, 1084
TasI	AATT	5 cut(s)	289, 466, 924, 951, 1193
TatI	WGTACW	2 cut(s)	242, 1174
TauI	GCSGC	1 cut(s)	1170
TfiI	GAWTC	2 cut(s)	119, 296
Tru1I	TTAA	5 cut(s)	113, 330, 371, 753, 786
Tru9I	TTAA	5 cut(s)	113, 330, 371, 753, 786
TscAI	CASTG	2 cut(s)	97, 1119
TseFI	GTSAC	2 cut(s)	625, 833
TseI	GCWGC	6 cut(s)	169, 316, 485, 591, 643, 1202
Tsp45I	GTSAC	2 cut(s)	625, 833
TspDTI	ATGAA	1 cut(s)	861
TspRI	CASTG	2 cut(s)	97, 1119
VpaK11BI	GGWCC	1 cut(s)	4
XapI	RAATTY	2 cut(s)	466, 1193
XhoI	CTCGAG	1 cut(s)	1083
XmiI	GTMKAC	3 cut(s)	50, 410, 531
XmnI	GAANNNNTTC	1 cut(s)	852
XspI	CTAG	2 cut(s)	729, 960
ZrmI	AGTACT	1 cut(s)	1176

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.