RchiOBHm_Chr4g0432381

Belongs to the actin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0008021
Physical Location & Seq
Reverse (-)
56551377 .. 56556736
5360 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40099

Sequence Viewer

Length: 1284 bp
ATGGACCCTGCCTCTCGCCCCGCAGTCGTCATTGACAACGGCACCGGGTATACTAAGATGGGGTTCGCCGGAAACGTCGAGCCGTGTTACATTGTCCCCACTGTAGTTGCTGTCAACGAATCGTTTCTGAACCAATCGAGAAGCACCTCCAAGGCGAGCTGGATAGCGCAGCACAATGCCGGTGTGATGGCTGATCTTGATTTCTTCATAGGGGATGAGGCTTTGGAGAAGTCTCGATCCAGCTCTACTTATAATTTGAGCTATCCGATCAGTCATGGGCAGGTGGACAATTGGGATTCAATGGAGCGCTTTTGGCAGCAGTGTATATTTAACTATTTGCGTTGTGATCCTGAAGATCATTACTTTCTTTTGACTGAGAGCCCGCTTACTGCCCCGGAGAGCCGGGAGTACACCGGCGAAATTATGTTTGAGACTTTCAATGTTCCTGGCCTTTACATTGCTGTGAATTCGGTACTTGCTCTCGCAGCTGGCTACACTACCTCTAAGTGTGAGATGACAGGTGTTGTAGTAGATGTTGGAGATGGGGCAAGTCATGTTGTTCCTGTTGCAGAAGGTTATGTGATTGGGAGCAGCATCAAGTCAGTTCCGATTGCCGGGAGAGATGTCACTCTCTTTGTTCAACAGCTTATGCGGGAAAGAGGAGAGAACGTGCCACCAGAAGACTCCTTTGATGTAGCTCGCAAAGTGAAGGAAATGTATTGCTATACTAGTTCTGACATTGTCAAGGAGTTCAATAAGCACGATAAAGAACCATCCAAATATATTAAGCAATGGAAAGGCATTAAACCAAAGACAGGGGCACCCTACACTTGTGATATTGGCTATGAACGATTTCTCGGACCAGAGGTATTTTTTAATCCTGAGATTTATAGCAGCGATTTCACGACTCCATTACCAGTTGTAATTGACAAGTGTATTCAGTCCGCCCCAATTGACACCAGGAGGTCTTTGTATAAGAATATCGTGTTGTCTGGAGGTTCAACGATGTTCAAGGACTTCCATAGAAGGCTGCAACGAGATATAAAGAAGATTGTGGATGCCCGGCTTCGTGCATCTGAAGCTCGAGTCGGTGGAGAACTAAAATCACATCCAGTGGAAGTAAATGTGGTAAGCCATCCCATCCAGAGATATGCAGTTTGGTTTGGAGGCTCCGTACTTGCATCCACGCCTGAATTCTTTGGGGCTTGCCATACTAAAGCAGAGTATGAGGAATATGGGGCAAGCATCTGTAGAACAAATCCTGTTTTCAAGGGGATGTACTGA

Protein Analysis

427

Amino Acids

47.68

Weight (kDa)

5.69

Isoelectric Point (pI)

38.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Actin PF00022 7 - 418 1.2e-79 Actin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 252
AarI CACCTGC 1 cut(s) 271
Acc36I ACCTGC 1 cut(s) 271
AccB1I GGYRCC 2 cut(s) 41, 820
AccI GTMKAC 1 cut(s) 50
AciI CCGC 4 cut(s) 21, 383, 652, 945
AclWI GGATC 2 cut(s) 231, 341
AcsI RAATTY 2 cut(s) 466, 1193
AcuI CTGAAG 2 cut(s) 372, 1098
AfaI GTAC 4 cut(s) 410, 474, 1176, 1280
AfeI AGCGCT 1 cut(s) 308
AfiI CCNNNNNNNGG 2 cut(s) 614, 815
AgsI TTSAA 7 cut(s) 300, 439, 641, 754, 1002, 1012, 1270
AhlI ACTAGT 1 cut(s) 728
AjnI CCWGG 2 cut(s) 445, 959
AluBI AGCT 7 cut(s) 159, 243, 261, 488, 646, 698, 1082
AluI AGCT 7 cut(s) 159, 243, 261, 488, 646, 698, 1082
Alw26I GTCTC 2 cut(s) 237, 425
AlwI GGATC 2 cut(s) 231, 341
Ama87I CYCGRG 1 cut(s) 1083
Aor51HI AGCGCT 1 cut(s) 308
AoxI GGCC 1 cut(s) 448
ApeKI GCWGC 6 cut(s) 169, 316, 485, 591, 894, 1030
ApoI RAATTY 2 cut(s) 466, 1193
Asp700I GAANNNNTTC 2 cut(s) 123, 852
AspLEI GCGC 2 cut(s) 169, 309
AspS9I GGNCC 2 cut(s) 4, 860
AsuC2I CCSGG 5 cut(s) 46, 395, 404, 616, 1063
AvaI CYCGRG 1 cut(s) 1083
AvaII GGWCC 2 cut(s) 4, 860
BaeGI GKGCMC 1 cut(s) 823
BanI GGYRCC 2 cut(s) 41, 820
BanII GRGCYC 1 cut(s) 383
BbsI GAAGAC 1 cut(s) 687
BbvI GCAGC 6 cut(s) 181, 328, 497, 603, 906, 1017
BccI CCATC 6 cut(s) 52, 181, 536, 781, 1143, 1148
BceAI ACGGC 2 cut(s) 55, 67
BciT130I CCWGG 2 cut(s) 447, 961
BcnI CCSGG 5 cut(s) 46, 395, 404, 616, 1063
BcoDI GTCTC 2 cut(s) 237, 425
BcuI ACTAGT 1 cut(s) 728
BfaI CTAG 1 cut(s) 729
BfmI CTRYAG 2 cut(s) 102, 1249
BfoI RGCGCY 1 cut(s) 310
BfuAI ACCTGC 1 cut(s) 271
BisI GCNGC 6 cut(s) 170, 317, 486, 592, 895, 1031
BlsI GCNGC 6 cut(s) 171, 318, 487, 593, 896, 1032
Bme1390I CCNGG 7 cut(s) 46, 395, 404, 447, 616, 961, 1063
Bme18I GGWCC 2 cut(s) 4, 860
BmeT110I CYCGRG 1 cut(s) 1083
BmgT120I GGNCC 2 cut(s) 4, 860
BmiI GGNNCC 4 cut(s) 6, 43, 822, 1171
BmrFI CCNGG 7 cut(s) 46, 395, 404, 447, 616, 961, 1063
BmsI GCATC 5 cut(s) 603, 1048, 1082, 1190, 1254
BpiI GAAGAC 1 cut(s) 687
BpmI CTGGAG 1 cut(s) 1014
BpuMI CCSGG 5 cut(s) 46, 395, 404, 616, 1063
BsaJI CCNNGG 2 cut(s) 150, 393
BsaXI ACNNNNNCTCC 4 cut(s) 654, 684, 1158, 1188
Bsc4I CCNNNNNNNGG 2 cut(s) 614, 815
Bse118I RCCGGY 2 cut(s) 179, 413
Bse1I ACTGG 2 cut(s) 917, 1112
Bse3DI GCAATG 2 cut(s) 456, 797
BseBI CCWGG 2 cut(s) 447, 961
BseDI CCNNGG 2 cut(s) 150, 393
BseGI GGATG 8 cut(s) 220, 773, 1063, 1108, 1135, 1140, 1181, 1281
BseLI CCNNNNNNNGG 2 cut(s) 614, 815
BseMI GCAATG 2 cut(s) 456, 797
BseMII CTCAG 2 cut(s) 366, 873
BseNI ACTGG 2 cut(s) 917, 1112
BseRI GAGGAG 1 cut(s) 675
BseSI GKGCMC 1 cut(s) 823
BseXI GCAGC 6 cut(s) 181, 328, 497, 603, 906, 1017
BshFI GGCC 1 cut(s) 450
BshNI GGYRCC 2 cut(s) 41, 820
BsiHKCI CYCGRG 1 cut(s) 1083
BsiSI CCGG 8 cut(s) 45, 69, 180, 395, 403, 414, 615, 1063
BslFI GGGAC 1 cut(s) 80
BslI CCNNNNNNNGG 2 cut(s) 614, 815
BsmAI GTCTC 2 cut(s) 237, 425
BsmFI GGGAC 1 cut(s) 80
BsnI GGCC 1 cut(s) 450
BsoBI CYCGRG 1 cut(s) 1083
Bsp1286I GDGCHC 2 cut(s) 383, 823
Bsp143I GATC 5 cut(s) 193, 236, 267, 346, 355
BspACI CCGC 4 cut(s) 21, 383, 652, 945
BspANI GGCC 1 cut(s) 450
BspCNI CTCAG 2 cut(s) 367, 874
BspLI GGNNCC 4 cut(s) 6, 43, 822, 1171
BspMI ACCTGC 1 cut(s) 271
BspPI GGATC 2 cut(s) 231, 341
BspT107I GGYRCC 2 cut(s) 41, 820
BsrDI GCAATG 2 cut(s) 456, 797
BsrFI RCCGGY 2 cut(s) 179, 413
BsrI ACTGG 2 cut(s) 917, 1112
BssAI RCCGGY 2 cut(s) 179, 413
BssECI CCNNGG 2 cut(s) 150, 393
BssMI GATC 5 cut(s) 193, 236, 267, 346, 355
BssNAI GTATAC 1 cut(s) 51
BssT1I CCWWGG 1 cut(s) 150
Bst1107I GTATAC 1 cut(s) 51
Bst2UI CCWGG 2 cut(s) 447, 961
Bst4CI ACNGT 1 cut(s) 103
BstC8I GCNNGC 6 cut(s) 157, 383, 490, 700, 1207, 1243
BstDEI CTNAG 4 cut(s) 54, 375, 504, 882
BstF5I GGATG 8 cut(s) 220, 773, 1063, 1108, 1135, 1140, 1181, 1281
BstH2I RGCGCY 1 cut(s) 310
BstHHI GCGC 2 cut(s) 169, 309
BstKTI GATC 5 cut(s) 196, 239, 270, 349, 358
BstMAI GTCTC 2 cut(s) 237, 425
BstMBI GATC 5 cut(s) 193, 236, 267, 346, 355
BstMWI GCNNNNNNNGC 3 cut(s) 313, 485, 1079
BstNI CCWGG 2 cut(s) 447, 961
BstSCI CCNGG 7 cut(s) 44, 393, 402, 445, 614, 959, 1061
BstSFI CTRYAG 2 cut(s) 102, 1249
BstSLI GKGCMC 1 cut(s) 823
BstV1I GCAGC 6 cut(s) 181, 328, 497, 603, 906, 1017
BstV2I GAAGAC 1 cut(s) 687
BstZ17I GTATAC 1 cut(s) 51
BsuRI GGCC 1 cut(s) 450
BtsCI GGATG 8 cut(s) 220, 773, 1063, 1108, 1135, 1140, 1181, 1281
BtsI GCAGTG 1 cut(s) 326
BtsIMutI CAGTG 3 cut(s) 99, 326, 1119
BveI ACCTGC 1 cut(s) 271
Cac8I GCNNGC 6 cut(s) 157, 383, 490, 700, 1207, 1243
CfoI GCGC 2 cut(s) 169, 309
Cfr10I RCCGGY 2 cut(s) 179, 413
Cfr13I GGNCC 2 cut(s) 4, 860
Csp6I GTAC 4 cut(s) 409, 473, 1175, 1279
CspCI CAANNNNNGTGG 2 cut(s) 88, 123
CviAII CATG 2 cut(s) 275, 554
CviQI GTAC 4 cut(s) 409, 473, 1175, 1279
DdeI CTNAG 4 cut(s) 54, 375, 504, 882
DpnI GATC 5 cut(s) 195, 238, 269, 348, 357
DpnII GATC 5 cut(s) 193, 236, 267, 346, 355
EciI GGCGGA 1 cut(s) 934
Eco130I CCWWGG 1 cut(s) 150
Eco24I GRGCYC 1 cut(s) 383
Eco47I GGWCC 2 cut(s) 4, 860
Eco47III AGCGCT 1 cut(s) 308
Eco57I CTGAAG 2 cut(s) 372, 1098
Eco88I CYCGRG 1 cut(s) 1083
EcoRI GAATTC 2 cut(s) 466, 1193
EcoRII CCWGG 2 cut(s) 445, 959
EcoT14I CCWWGG 1 cut(s) 150
EcoT38I GRGCYC 1 cut(s) 383
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 2 cut(s) 278, 557
FaqI GGGAC 1 cut(s) 80
FatI CATG 2 cut(s) 274, 553
FauI CCCGC 3 cut(s) 28, 390, 645
FblI GTMKAC 1 cut(s) 50
Fnu4HI GCNGC 6 cut(s) 170, 317, 486, 592, 895, 1031
FokI GGATG 7 cut(s) 227, 760, 1070, 1095, 1122, 1127, 1168
FriOI GRGCYC 1 cut(s) 383
Fsp4HI GCNGC 6 cut(s) 170, 317, 486, 592, 895, 1031
FspBI CTAG 1 cut(s) 729
GlaI GCGC 2 cut(s) 168, 308
GluI GCNGC 6 cut(s) 170, 317, 486, 592, 895, 1031
GsuI CTGGAG 1 cut(s) 1014
HaeII RGCGCY 1 cut(s) 310
HaeIII GGCC 1 cut(s) 450
HapII CCGG 8 cut(s) 45, 69, 180, 395, 403, 414, 615, 1063
HhaI GCGC 2 cut(s) 169, 309
Hin1II CATG 2 cut(s) 278, 557
Hin6I GCGC 2 cut(s) 167, 307
HinP1I GCGC 2 cut(s) 167, 307
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HinfI GANTC 5 cut(s) 119, 296, 683, 907, 1086
HpaII CCGG 8 cut(s) 45, 69, 180, 395, 403, 414, 615, 1063
Hpy166II GTNNAC 4 cut(s) 51, 115, 286, 411
Hpy188I TCNGA 6 cut(s) 129, 267, 609, 736, 860, 1078
Hpy188III TCNNGA 8 cut(s) 138, 197, 234, 350, 881, 904, 993, 1144
Hpy8I GTNNAC 4 cut(s) 51, 115, 286, 411
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 3 cut(s) 566, 703, 1020
HpyCH4III ACNGT 1 cut(s) 103
HpyCH4IV ACGT 2 cut(s) 75, 669
HpyCH4V TGCA 5 cut(s) 569, 1033, 1073, 1154, 1181
HpyF10VI GCNNNNNNNGC 3 cut(s) 313, 485, 1079
HpyF3I CTNAG 4 cut(s) 54, 375, 504, 882
HpySE526I ACGT 2 cut(s) 75, 669
Hsp92II CATG 2 cut(s) 278, 557
HspAI GCGC 2 cut(s) 167, 307
Kzo9I GATC 5 cut(s) 193, 236, 267, 346, 355
LmnI GCTCC 3 cut(s) 304, 588, 1175
Lsp1109I GCAGC 6 cut(s) 181, 328, 497, 603, 906, 1017
LweI GCATC 5 cut(s) 603, 1048, 1082, 1190, 1254
MaeI CTAG 1 cut(s) 729
MaeII ACGT 2 cut(s) 75, 669
MaeIII GTNAC 2 cut(s) 86, 625
MalI GATC 5 cut(s) 195, 238, 269, 348, 357
MboI GATC 5 cut(s) 193, 236, 267, 346, 355
MboII GAAGA 4 cut(s) 196, 365, 692, 1060
MfeI CAATTG 2 cut(s) 289, 951
MhlI GDGCHC 2 cut(s) 383, 823
MluCI AATT 7 cut(s) 253, 289, 420, 466, 924, 951, 1193
MlyI GAGTC 3 cut(s) 677, 901, 1095
MmeI TCCRAC 1 cut(s) 517
MroXI GAANNNNTTC 2 cut(s) 123, 852
MseI TTAA 4 cut(s) 330, 786, 804, 876
MslI CAYNNNNRTG 1 cut(s) 461
MspA1I CMGCKG 1 cut(s) 488
MspI CCGG 8 cut(s) 45, 69, 180, 395, 403, 414, 615, 1063
MspR9I CCNGG 7 cut(s) 46, 395, 404, 447, 616, 961, 1063
MunI CAATTG 2 cut(s) 289, 951
MvaI CCWGG 2 cut(s) 447, 961
MwoI GCNNNNNNNGC 3 cut(s) 313, 485, 1079
NciI CCSGG 5 cut(s) 46, 395, 404, 616, 1063
NdeII GATC 5 cut(s) 193, 236, 267, 346, 355
NlaIII CATG 2 cut(s) 278, 557
NlaIV GGNNCC 4 cut(s) 6, 43, 822, 1171
NmuCI GTSAC 1 cut(s) 625
PaeR7I CTCGAG 1 cut(s) 1083
PaqCI CACCTGC 1 cut(s) 271
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PdmI GAANNNNTTC 2 cut(s) 123, 852
PfeI GAWTC 2 cut(s) 119, 296
PflFI GACNNNGTC 1 cut(s) 740
PkrI GCNGC 6 cut(s) 171, 318, 487, 593, 896, 1032
PleI GAGTC 3 cut(s) 677, 901, 1094
PpsI GAGTC 3 cut(s) 677, 901, 1094
PsiI TTATAA 1 cut(s) 252
Psp6I CCWGG 2 cut(s) 445, 959
PspGI CCWGG 2 cut(s) 445, 959
PspN4I GGNNCC 4 cut(s) 6, 43, 822, 1171
PspPI GGNCC 2 cut(s) 4, 860
PspXI VCTCGAGB 1 cut(s) 1083
PsyI GACNNNGTC 1 cut(s) 740
PvuII CAGCTG 1 cut(s) 488
RsaI GTAC 4 cut(s) 410, 474, 1176, 1280
RsaNI GTAC 4 cut(s) 409, 473, 1175, 1279
RseI CAYNNNNRTG 1 cut(s) 461
SaqAI TTAA 4 cut(s) 330, 786, 804, 876
SatI GCNGC 6 cut(s) 170, 317, 486, 592, 895, 1031
Sau3AI GATC 5 cut(s) 193, 236, 267, 346, 355
Sau96I GGNCC 2 cut(s) 4, 860
SchI GAGTC 3 cut(s) 677, 901, 1095
ScrFI CCNGG 7 cut(s) 46, 395, 404, 447, 616, 961, 1063
SduI GDGCHC 2 cut(s) 383, 823
SfaNI GCATC 5 cut(s) 603, 1048, 1082, 1190, 1254
SfcI CTRYAG 2 cut(s) 102, 1249
Sfr274I CTCGAG 1 cut(s) 1083
SgrAI CRCCGGYG 1 cut(s) 413
SinI GGWCC 2 cut(s) 4, 860
SlaI CTCGAG 1 cut(s) 1083
SmiMI CAYNNNNRTG 1 cut(s) 461
SmlI CTYRAG 1 cut(s) 1083
SmoI CTYRAG 1 cut(s) 1083
SpeI ACTAGT 1 cut(s) 728
Sse9I AATT 7 cut(s) 253, 289, 420, 466, 924, 951, 1193
SsiI CCGC 4 cut(s) 21, 383, 652, 945
SspMI CTAG 1 cut(s) 729
StyD4I CCNGG 7 cut(s) 44, 393, 402, 445, 614, 959, 1061
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 1 cut(s) 103
TaiI ACGT 2 cut(s) 78, 672
TaqI TCGA 4 cut(s) 78, 137, 235, 1084
TasI AATT 7 cut(s) 253, 289, 420, 466, 924, 951, 1193
TatI WGTACW 2 cut(s) 408, 1278
TfiI GAWTC 2 cut(s) 119, 296
Tru1I TTAA 4 cut(s) 330, 786, 804, 876
Tru9I TTAA 4 cut(s) 330, 786, 804, 876
TscAI CASTG 3 cut(s) 106, 326, 1119
TseFI GTSAC 1 cut(s) 625
TseI GCWGC 6 cut(s) 169, 316, 485, 591, 894, 1030
Tsp45I GTSAC 1 cut(s) 625
TspDTI ATGAA 2 cut(s) 196, 861
TspGWI ACGGA 1 cut(s) 1162
TspRI CASTG 3 cut(s) 106, 326, 1119
Tth111I GACNNNGTC 1 cut(s) 740
VpaK11BI GGWCC 2 cut(s) 4, 860
XapI RAATTY 2 cut(s) 466, 1193
XhoI CTCGAG 1 cut(s) 1083
XmiI GTMKAC 1 cut(s) 50
XmnI GAANNNNTTC 2 cut(s) 123, 852
XspI CTAG 1 cut(s) 729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.