Rh4DG317000

Belongs to the actin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0008021
Physical Location & Seq
Reverse (-)
53990570 .. 53995418
4849 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG317000.1

Sequence Viewer

Length: 1320 bp
ATGGACCCTGCCTCTCGCCCCGCAGTCGTCATTGACAACGGCACCGGGTATACTAAGATGGGGTTCGCCGGAAACGTCGAGCCGTGTTACATTGTCCCCACTGTAGTTGCTGTCAACGAATCGTTTCTGAACCAATCGAGAAGCACCTCCAAGGCGAGCTGGATAGCGCAGCACAATGCCGGTGTGATGGCTGATCTTGATTTCTTCATAGGGGATGAGGCTTTGGAGAAGTCTCGATCCAGCTCTACTTATAATTTGAGCTATCCGATCAGTCATGGGCAGGTGGACAATTGGGATTCAATGGAGCGCTTTTGGCAGCAGTGTATATTTAACTATTTGCGTTGTGATCCTGAAGATCATTACTTTCTTTTGACTGAGAGCCCGCTTACTGCCCCGGAGAGCCGGGAGTACACCGGCGAAATTATGTTTGAGACTTTCAATGTTCCTGGCCTTTACATTGCTGTGAATTCGGTACTTGCTCTCGCAGCTGGCTACACTACCTCTAAGTGTGAGATGACAGGTGTTGTAGTAGATGTTGGAGATGGGGCAAGTCATGTTGTTCCTGTTGCAGAAGGTTATGTGATTGGGAGCAGCATCAAGTCAGTTCCGATTGCCGGGAGAGATGTCACTCTCTTTGTTCAACAGCTTATGCGGGAAAGAGGAGAGAACGTGCCACCAGAAGACTCCTTTGATGTAGCTCGCAAAGTGAAGGAAATGTATTGCTATACTAGTTCTGACATTGTCAAGGAGTTCAATAAGCACGATAAAGAACCATCCAAATATATTAAGCAATGGAAAGGCATTAAACCAAAGACAGGGGCACCCTACACTTGTGATATTGGCTATGAACGATTTCTCGGACCAGAGGTATTTTTTAATCCTGAGATTTATAGCAGCGATTTCACGACTCCATTACCAGTTGTAATTGACAAGTGTATTCAGTCCGCCCCAATTGACACCAGGAGGTCTTTGTATAAGAATATCGTGTTGTCTGGAGGTTCAACGATGTTCAAGGACTTCCATAGAAGGCTGCAACGAGATATAAAGAAGATTGTGGATGCCCGGCTTCGTGCATCTGAAGCTCGAGTCGGTGGAGAACTAAAAGTAAGCTTTTTTTTTTGTTTTGTTTGCATTATGTATTTGTCTATGAATTTGTTAAGTTTCAGTCTCATCACAGTCAATGCATTTGATTTTTATGTTTGTCTGGGTCTGCAGTCACATCCAGTGGAAGTAAATGTGGTAAGCCATCCCATCCAGAGATATGCAGTTTGGTTTGGAGGCTCCGTACTTGCATCCACGCCTGAATTCTTTGGGGTATGA

Protein Analysis

439

Amino Acids

49.1

Weight (kDa)

5.49

Isoelectric Point (pI)

39.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Actin PF00022 7 - 357 2e-66 Actin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 252
AarI CACCTGC 1 cut(s) 271
Acc36I ACCTGC 1 cut(s) 271
AccB1I GGYRCC 2 cut(s) 41, 820
AccI GTMKAC 1 cut(s) 50
AciI CCGC 4 cut(s) 21, 383, 652, 945
AclWI GGATC 2 cut(s) 231, 341
AcsI RAATTY 3 cut(s) 466, 1150, 1304
AcuI CTGAAG 2 cut(s) 372, 1098
AfaI GTAC 3 cut(s) 410, 474, 1287
AfeI AGCGCT 1 cut(s) 308
AfiI CCNNNNNNNGG 2 cut(s) 614, 815
AgsI TTSAA 6 cut(s) 300, 439, 641, 754, 1002, 1012
AhlI ACTAGT 1 cut(s) 728
AjnI CCWGG 2 cut(s) 445, 959
AluBI AGCT 8 cut(s) 159, 243, 261, 488, 646, 698, 1082, 1110
AluI AGCT 8 cut(s) 159, 243, 261, 488, 646, 698, 1082, 1110
Alw26I GTCTC 3 cut(s) 237, 425, 1172
AlwI GGATC 2 cut(s) 231, 341
Ama87I CYCGRG 1 cut(s) 1083
Aor51HI AGCGCT 1 cut(s) 308
AoxI GGCC 1 cut(s) 448
ApeKI GCWGC 6 cut(s) 169, 316, 485, 591, 894, 1030
ApoI RAATTY 3 cut(s) 466, 1150, 1304
Asp700I GAANNNNTTC 2 cut(s) 123, 852
AspLEI GCGC 2 cut(s) 169, 309
AspS9I GGNCC 2 cut(s) 4, 860
AsuC2I CCSGG 5 cut(s) 46, 395, 404, 616, 1063
AvaI CYCGRG 1 cut(s) 1083
AvaII GGWCC 2 cut(s) 4, 860
BaeGI GKGCMC 1 cut(s) 823
BanI GGYRCC 2 cut(s) 41, 820
BanII GRGCYC 1 cut(s) 383
BbsI GAAGAC 1 cut(s) 687
BbvI GCAGC 6 cut(s) 181, 328, 497, 603, 906, 1017
BccI CCATC 6 cut(s) 52, 181, 536, 781, 1254, 1259
BceAI ACGGC 2 cut(s) 55, 67
BciT130I CCWGG 2 cut(s) 447, 961
BcnI CCSGG 5 cut(s) 46, 395, 404, 616, 1063
BcoDI GTCTC 3 cut(s) 237, 425, 1172
BcuI ACTAGT 1 cut(s) 728
BfaI CTAG 1 cut(s) 729
BfmI CTRYAG 2 cut(s) 102, 1211
BfoI RGCGCY 1 cut(s) 310
BfuAI ACCTGC 1 cut(s) 271
BisI GCNGC 6 cut(s) 170, 317, 486, 592, 895, 1031
BlsI GCNGC 6 cut(s) 171, 318, 487, 593, 896, 1032
Bme1390I CCNGG 7 cut(s) 46, 395, 404, 447, 616, 961, 1063
Bme18I GGWCC 2 cut(s) 4, 860
BmeT110I CYCGRG 1 cut(s) 1083
BmgT120I GGNCC 2 cut(s) 4, 860
BmiI GGNNCC 4 cut(s) 6, 43, 822, 1282
BmrFI CCNGG 7 cut(s) 46, 395, 404, 447, 616, 961, 1063
BmsI GCATC 4 cut(s) 603, 1048, 1082, 1301
BpiI GAAGAC 1 cut(s) 687
BpmI CTGGAG 1 cut(s) 1014
BpuMI CCSGG 5 cut(s) 46, 395, 404, 616, 1063
BsaJI CCNNGG 2 cut(s) 150, 393
BsaXI ACNNNNNCTCC 4 cut(s) 654, 684, 1269, 1299
Bsc4I CCNNNNNNNGG 2 cut(s) 614, 815
Bse118I RCCGGY 2 cut(s) 179, 413
Bse1I ACTGG 2 cut(s) 917, 1223
Bse3DI GCAATG 2 cut(s) 456, 797
BseBI CCWGG 2 cut(s) 447, 961
BseDI CCNNGG 2 cut(s) 150, 393
BseGI GGATG 7 cut(s) 220, 773, 1063, 1219, 1246, 1251, 1292
BseLI CCNNNNNNNGG 2 cut(s) 614, 815
BseMI GCAATG 2 cut(s) 456, 797
BseMII CTCAG 2 cut(s) 366, 873
BseNI ACTGG 2 cut(s) 917, 1223
BseRI GAGGAG 1 cut(s) 675
BseSI GKGCMC 1 cut(s) 823
BseXI GCAGC 6 cut(s) 181, 328, 497, 603, 906, 1017
BshFI GGCC 1 cut(s) 450
BshNI GGYRCC 2 cut(s) 41, 820
BsiHKCI CYCGRG 1 cut(s) 1083
BsiSI CCGG 8 cut(s) 45, 69, 180, 395, 403, 414, 615, 1063
BslFI GGGAC 1 cut(s) 80
BslI CCNNNNNNNGG 2 cut(s) 614, 815
BsmAI GTCTC 3 cut(s) 237, 425, 1172
BsmFI GGGAC 1 cut(s) 80
BsnI GGCC 1 cut(s) 450
BsoBI CYCGRG 1 cut(s) 1083
Bsp1286I GDGCHC 2 cut(s) 383, 823
Bsp143I GATC 5 cut(s) 193, 236, 267, 346, 355
BspACI CCGC 4 cut(s) 21, 383, 652, 945
BspANI GGCC 1 cut(s) 450
BspCNI CTCAG 2 cut(s) 367, 874
BspLI GGNNCC 4 cut(s) 6, 43, 822, 1282
BspMAI CTGCAG 1 cut(s) 1215
BspMI ACCTGC 1 cut(s) 271
BspPI GGATC 2 cut(s) 231, 341
BspT107I GGYRCC 2 cut(s) 41, 820
BsrDI GCAATG 2 cut(s) 456, 797
BsrFI RCCGGY 2 cut(s) 179, 413
BsrI ACTGG 2 cut(s) 917, 1223
BssAI RCCGGY 2 cut(s) 179, 413
BssECI CCNNGG 2 cut(s) 150, 393
BssMI GATC 5 cut(s) 193, 236, 267, 346, 355
BssNAI GTATAC 1 cut(s) 51
BssT1I CCWWGG 1 cut(s) 150
Bst1107I GTATAC 1 cut(s) 51
Bst2UI CCWGG 2 cut(s) 447, 961
Bst4CI ACNGT 2 cut(s) 103, 1177
BstC8I GCNNGC 4 cut(s) 157, 383, 490, 700
BstDEI CTNAG 4 cut(s) 54, 375, 504, 882
BstF5I GGATG 7 cut(s) 220, 773, 1063, 1219, 1246, 1251, 1292
BstH2I RGCGCY 1 cut(s) 310
BstHHI GCGC 2 cut(s) 169, 309
BstKTI GATC 5 cut(s) 196, 239, 270, 349, 358
BstMAI GTCTC 3 cut(s) 237, 425, 1172
BstMBI GATC 5 cut(s) 193, 236, 267, 346, 355
BstMWI GCNNNNNNNGC 3 cut(s) 313, 485, 1079
BstNI CCWGG 2 cut(s) 447, 961
BstSCI CCNGG 7 cut(s) 44, 393, 402, 445, 614, 959, 1061
BstSFI CTRYAG 2 cut(s) 102, 1211
BstSLI GKGCMC 1 cut(s) 823
BstV1I GCAGC 6 cut(s) 181, 328, 497, 603, 906, 1017
BstV2I GAAGAC 1 cut(s) 687
BstZ17I GTATAC 1 cut(s) 51
BsuRI GGCC 1 cut(s) 450
BtsCI GGATG 7 cut(s) 220, 773, 1063, 1219, 1246, 1251, 1292
BtsI GCAGTG 1 cut(s) 326
BtsIMutI CAGTG 3 cut(s) 99, 326, 1230
BveI ACCTGC 1 cut(s) 271
Cac8I GCNNGC 4 cut(s) 157, 383, 490, 700
CfoI GCGC 2 cut(s) 169, 309
Cfr10I RCCGGY 2 cut(s) 179, 413
Cfr13I GGNCC 2 cut(s) 4, 860
Csp6I GTAC 3 cut(s) 409, 473, 1286
CspCI CAANNNNNGTGG 2 cut(s) 88, 123
CviAII CATG 2 cut(s) 275, 554
CviQI GTAC 3 cut(s) 409, 473, 1286
DdeI CTNAG 4 cut(s) 54, 375, 504, 882
DpnI GATC 5 cut(s) 195, 238, 269, 348, 357
DpnII GATC 5 cut(s) 193, 236, 267, 346, 355
EciI GGCGGA 1 cut(s) 934
Eco130I CCWWGG 1 cut(s) 150
Eco24I GRGCYC 1 cut(s) 383
Eco47I GGWCC 2 cut(s) 4, 860
Eco47III AGCGCT 1 cut(s) 308
Eco57I CTGAAG 2 cut(s) 372, 1098
Eco88I CYCGRG 1 cut(s) 1083
EcoRI GAATTC 2 cut(s) 466, 1304
EcoRII CCWGG 2 cut(s) 445, 959
EcoT14I CCWWGG 1 cut(s) 150
EcoT22I ATGCAT 1 cut(s) 1186
EcoT38I GRGCYC 1 cut(s) 383
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 2 cut(s) 278, 557
FaqI GGGAC 1 cut(s) 80
FatI CATG 2 cut(s) 274, 553
FauI CCCGC 3 cut(s) 28, 390, 645
FblI GTMKAC 1 cut(s) 50
Fnu4HI GCNGC 6 cut(s) 170, 317, 486, 592, 895, 1031
FokI GGATG 7 cut(s) 227, 760, 1070, 1206, 1233, 1238, 1279
FriOI GRGCYC 1 cut(s) 383
Fsp4HI GCNGC 6 cut(s) 170, 317, 486, 592, 895, 1031
FspBI CTAG 1 cut(s) 729
GlaI GCGC 2 cut(s) 168, 308
GluI GCNGC 6 cut(s) 170, 317, 486, 592, 895, 1031
GsuI CTGGAG 1 cut(s) 1014
HaeII RGCGCY 1 cut(s) 310
HaeIII GGCC 1 cut(s) 450
HapII CCGG 8 cut(s) 45, 69, 180, 395, 403, 414, 615, 1063
HhaI GCGC 2 cut(s) 169, 309
Hin1II CATG 2 cut(s) 278, 557
Hin6I GCGC 2 cut(s) 167, 307
HinP1I GCGC 2 cut(s) 167, 307
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HindIII AAGCTT 1 cut(s) 1108
HinfI GANTC 5 cut(s) 119, 296, 683, 907, 1086
HpaII CCGG 8 cut(s) 45, 69, 180, 395, 403, 414, 615, 1063
Hpy166II GTNNAC 4 cut(s) 51, 115, 286, 411
Hpy188I TCNGA 6 cut(s) 129, 267, 609, 736, 860, 1078
Hpy188III TCNNGA 8 cut(s) 138, 197, 234, 350, 881, 904, 993, 1255
Hpy8I GTNNAC 4 cut(s) 51, 115, 286, 411
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 3 cut(s) 566, 703, 1020
HpyCH4III ACNGT 2 cut(s) 103, 1177
HpyCH4IV ACGT 2 cut(s) 75, 669
HpyCH4V TGCA 8 cut(s) 569, 1033, 1073, 1131, 1184, 1213, 1265, 1292
HpyF10VI GCNNNNNNNGC 3 cut(s) 313, 485, 1079
HpyF3I CTNAG 4 cut(s) 54, 375, 504, 882
HpySE526I ACGT 2 cut(s) 75, 669
Hsp92II CATG 2 cut(s) 278, 557
HspAI GCGC 2 cut(s) 167, 307
Kzo9I GATC 5 cut(s) 193, 236, 267, 346, 355
LmnI GCTCC 3 cut(s) 304, 588, 1286
Lsp1109I GCAGC 6 cut(s) 181, 328, 497, 603, 906, 1017
LweI GCATC 4 cut(s) 603, 1048, 1082, 1301
MaeI CTAG 1 cut(s) 729
MaeII ACGT 2 cut(s) 75, 669
MaeIII GTNAC 3 cut(s) 86, 625, 1215
MalI GATC 5 cut(s) 195, 238, 269, 348, 357
MboI GATC 5 cut(s) 193, 236, 267, 346, 355
MboII GAAGA 4 cut(s) 196, 365, 692, 1060
MfeI CAATTG 2 cut(s) 289, 951
MhlI GDGCHC 2 cut(s) 383, 823
MluCI AATT 8 cut(s) 253, 289, 420, 466, 924, 951, 1150, 1304
MlyI GAGTC 3 cut(s) 677, 901, 1095
MmeI TCCRAC 1 cut(s) 517
MnlI CCTC 9 cut(s) 22, 157, 211, 511, 653, 859, 957, 989, 1271
Mph1103I ATGCAT 1 cut(s) 1186
MroXI GAANNNNTTC 2 cut(s) 123, 852
MseI TTAA 5 cut(s) 330, 786, 804, 876, 1157
MslI CAYNNNNRTG 1 cut(s) 461
MspA1I CMGCKG 1 cut(s) 488
MspI CCGG 8 cut(s) 45, 69, 180, 395, 403, 414, 615, 1063
MspR9I CCNGG 7 cut(s) 46, 395, 404, 447, 616, 961, 1063
MunI CAATTG 2 cut(s) 289, 951
MvaI CCWGG 2 cut(s) 447, 961
MwoI GCNNNNNNNGC 3 cut(s) 313, 485, 1079
NciI CCSGG 5 cut(s) 46, 395, 404, 616, 1063
NdeII GATC 5 cut(s) 193, 236, 267, 346, 355
NlaIII CATG 2 cut(s) 278, 557
NlaIV GGNNCC 4 cut(s) 6, 43, 822, 1282
NmuCI GTSAC 2 cut(s) 625, 1215
NsiI ATGCAT 1 cut(s) 1186
PaeR7I CTCGAG 1 cut(s) 1083
PaqCI CACCTGC 1 cut(s) 271
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PdmI GAANNNNTTC 2 cut(s) 123, 852
PfeI GAWTC 2 cut(s) 119, 296
PflFI GACNNNGTC 1 cut(s) 740
PkrI GCNGC 6 cut(s) 171, 318, 487, 593, 896, 1032
PleI GAGTC 3 cut(s) 677, 901, 1094
PpsI GAGTC 3 cut(s) 677, 901, 1094
PsiI TTATAA 1 cut(s) 252
Psp6I CCWGG 2 cut(s) 445, 959
PspGI CCWGG 2 cut(s) 445, 959
PspN4I GGNNCC 4 cut(s) 6, 43, 822, 1282
PspPI GGNCC 2 cut(s) 4, 860
PspXI VCTCGAGB 1 cut(s) 1083
PstI CTGCAG 1 cut(s) 1215
PsyI GACNNNGTC 1 cut(s) 740
PvuII CAGCTG 1 cut(s) 488
RsaI GTAC 3 cut(s) 410, 474, 1287
RsaNI GTAC 3 cut(s) 409, 473, 1286
RseI CAYNNNNRTG 1 cut(s) 461
SaqAI TTAA 5 cut(s) 330, 786, 804, 876, 1157
SatI GCNGC 6 cut(s) 170, 317, 486, 592, 895, 1031
Sau3AI GATC 5 cut(s) 193, 236, 267, 346, 355
Sau96I GGNCC 2 cut(s) 4, 860
SchI GAGTC 3 cut(s) 677, 901, 1095
ScrFI CCNGG 7 cut(s) 46, 395, 404, 447, 616, 961, 1063
SduI GDGCHC 2 cut(s) 383, 823
SfaNI GCATC 4 cut(s) 603, 1048, 1082, 1301
SfcI CTRYAG 2 cut(s) 102, 1211
Sfr274I CTCGAG 1 cut(s) 1083
SgrAI CRCCGGYG 1 cut(s) 413
SinI GGWCC 2 cut(s) 4, 860
SlaI CTCGAG 1 cut(s) 1083
SmiMI CAYNNNNRTG 1 cut(s) 461
SmlI CTYRAG 1 cut(s) 1083
SmoI CTYRAG 1 cut(s) 1083
SpeI ACTAGT 1 cut(s) 728
Sse9I AATT 8 cut(s) 253, 289, 420, 466, 924, 951, 1150, 1304
SsiI CCGC 4 cut(s) 21, 383, 652, 945
SspMI CTAG 1 cut(s) 729
StyD4I CCNGG 7 cut(s) 44, 393, 402, 445, 614, 959, 1061
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 2 cut(s) 103, 1177
TaiI ACGT 2 cut(s) 78, 672
TaqI TCGA 4 cut(s) 78, 137, 235, 1084
TasI AATT 8 cut(s) 253, 289, 420, 466, 924, 951, 1150, 1304
TatI WGTACW 1 cut(s) 408
TfiI GAWTC 2 cut(s) 119, 296
Tru1I TTAA 5 cut(s) 330, 786, 804, 876, 1157
Tru9I TTAA 5 cut(s) 330, 786, 804, 876, 1157
TscAI CASTG 3 cut(s) 106, 326, 1230
TseFI GTSAC 2 cut(s) 625, 1215
TseI GCWGC 6 cut(s) 169, 316, 485, 591, 894, 1030
Tsp45I GTSAC 2 cut(s) 625, 1215
TspDTI ATGAA 3 cut(s) 196, 861, 1163
TspGWI ACGGA 1 cut(s) 1273
TspRI CASTG 3 cut(s) 106, 326, 1230
Tth111I GACNNNGTC 1 cut(s) 740
VpaK11BI GGWCC 2 cut(s) 4, 860
XapI RAATTY 3 cut(s) 466, 1150, 1304
XhoI CTCGAG 1 cut(s) 1083
XmiI GTMKAC 1 cut(s) 50
XmnI GAANNNNTTC 2 cut(s) 123, 852
XspI CTAG 1 cut(s) 729
Zsp2I ATGCAT 1 cut(s) 1186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.