MD16G1138400.v1.1

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0010833
Physical Location & Seq
Forward (+)
10612968 .. 10615106
2139 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1138400.v1.1.491

Sequence Viewer

Length: 357 bp
ATGGGGAAGATCCAATCTTTCAAGCAGGAGTTCTCATTCGATGAACGACTTGAAGAATCGAAAAGCATAATTGCAAAATACCCAGATCGAGTTCCGGTGATCATTGAAAGATATTCCAGGACAGACCTGCCTGAAATGGAAAAGAAAAAATTCCTGGTTCCTAGAGATATGTCTGTCGGGCAGTTTATCCATATCTTAAGCAGCAGGCTTCACTTGACCCCGGGGAAAGCGCTTTTTGTTTTTGTGAAGAACACTTTACCTCAAACAGCCGGTCGCTTGGATTCCATCTATGAAACTTACAAGGAGGATGACGGTTTCCTGTATATGTGTTACAGCAGCGAGAAAACCTTTGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04068 ko04136 ko04137 ko04138 ko04139 ko04140 ko04212 ko04371 ko04621 ko04727 ko05167 map04068 map04136 map04137 map04138 map04139 map04140 map04212 map04371 map04621 map04727 map05167
Pfam Domains
Protein Families
Atg8

Protein Analysis

119

Amino Acids

13.78

Weight (kDa)

7.81

Isoelectric Point (pI)

56.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG8 PF02991 15 - 118 4.1e-44 Autophagy protein Atg8 ubiquitin like
APG12 PF04110 41 - 118 9.5e-07 Ubiquitin-like autophagy protein Apg12
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010833)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06420
fragaria_vesca FvH4_4g21320 FvH4_4g21320
malus_domestica MD13G1141800.v1.1 MD16G1138400.v1.1
prunus_persica Prupe.1G107100_v2.0.a1 Prupe.1G107100_v2.0.a1
pyrus_communis pycom13g12340 pycom16g12230
rosa_chinensis RchiOBHm_Chr4g0427091
rosa_laevigata RLG00000007262
rosa_multiflora Rmu_sc0000953.1_g000030
rosa_roxburghii Rroxscaffold_5G00368920
rosa_rugosa Rorug04G0215900 Rorug04G0216000
rosa_samantha Rh4AG271700 Rh4BG277600 Rh4CG292300 Rh4DG275200
rosa_wichuraiana Rw4G023570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 135
AclWI GGATC 1 cut(s) 4
AcsI RAATTY 1 cut(s) 149
AfeI AGCGCT 1 cut(s) 231
AflII CTTAAG 1 cut(s) 196
AgsI TTSAA 3 cut(s) 22, 53, 107
AjnI CCWGG 2 cut(s) 116, 153
AlwI GGATC 1 cut(s) 4
Ama87I CYCGRG 1 cut(s) 220
Aor51HI AGCGCT 1 cut(s) 231
ApeKI GCWGC 2 cut(s) 201, 336
ApoI RAATTY 1 cut(s) 149
ArsI GACNNNNNNTTYG 2 cut(s) 256, 288
Asp700I GAANNNNTTC 1 cut(s) 149
AspLEI GCGC 1 cut(s) 232
AsuC2I CCSGG 2 cut(s) 221, 222
AsuHPI GGTGA 1 cut(s) 109
AvaI CYCGRG 1 cut(s) 220
BbvI GCAGC 2 cut(s) 213, 348
BccI CCATC 1 cut(s) 293
BciT130I CCWGG 2 cut(s) 118, 155
BclI TGATCA 1 cut(s) 99
BcnI CCSGG 2 cut(s) 221, 222
BfaI CTAG 1 cut(s) 162
BfoI RGCGCY 1 cut(s) 233
BfrI CTTAAG 1 cut(s) 196
BfuAI ACCTGC 1 cut(s) 135
BisI GCNGC 2 cut(s) 202, 337
BlsI GCNGC 2 cut(s) 203, 338
Bme1390I CCNGG 4 cut(s) 118, 155, 221, 222
BmeT110I CYCGRG 1 cut(s) 220
BmiI GGNNCC 1 cut(s) 159
BmrFI CCNGG 4 cut(s) 118, 155, 221, 222
BpuMI CCSGG 2 cut(s) 221, 222
BsaJI CCNNGG 3 cut(s) 219, 220, 221
BsaWI WCCGGW 1 cut(s) 94
Bse118I RCCGGY 1 cut(s) 269
BseBI CCWGG 2 cut(s) 118, 155
BseDI CCNNGG 3 cut(s) 219, 220, 221
BseGI GGATG 1 cut(s) 313
BseXI GCAGC 2 cut(s) 213, 348
Bsh1285I CGRYCG 1 cut(s) 274
BsiEI CGRYCG 1 cut(s) 274
BsiHKCI CYCGRG 1 cut(s) 220
BsiSI CCGG 3 cut(s) 95, 221, 270
BsoBI CYCGRG 1 cut(s) 220
Bsp143I GATC 3 cut(s) 9, 85, 99
BspLI GGNNCC 1 cut(s) 159
BspMI ACCTGC 1 cut(s) 135
BspPI GGATC 1 cut(s) 4
BspTI CTTAAG 1 cut(s) 196
BsrFI RCCGGY 1 cut(s) 269
BssAI RCCGGY 1 cut(s) 269
BssECI CCNNGG 3 cut(s) 219, 220, 221
BssMI GATC 3 cut(s) 9, 85, 99
Bst2UI CCWGG 2 cut(s) 118, 155
Bst4CI ACNGT 1 cut(s) 314
BstAFI CTTAAG 1 cut(s) 196
BstC8I GCNNGC 1 cut(s) 206
BstF5I GGATG 1 cut(s) 313
BstH2I RGCGCY 1 cut(s) 233
BstHHI GCGC 1 cut(s) 232
BstKTI GATC 3 cut(s) 12, 88, 102
BstMBI GATC 3 cut(s) 9, 85, 99
BstMCI CGRYCG 1 cut(s) 274
BstNI CCWGG 2 cut(s) 118, 155
BstSCI CCNGG 4 cut(s) 116, 153, 219, 220
BstV1I GCAGC 2 cut(s) 213, 348
BstX2I RGATCY 1 cut(s) 9
BstYI RGATCY 1 cut(s) 9
BtsCI GGATG 1 cut(s) 313
BveI ACCTGC 1 cut(s) 135
Cac8I GCNNGC 1 cut(s) 206
CfoI GCGC 1 cut(s) 232
Cfr10I RCCGGY 1 cut(s) 269
Cfr9I CCCGGG 1 cut(s) 220
CviJI RGCY 3 cut(s) 208, 269, 354
CviKI_1 RGCY 3 cut(s) 208, 269, 354
DpnI GATC 3 cut(s) 11, 87, 101
DpnII GATC 3 cut(s) 9, 85, 99
Eco47III AGCGCT 1 cut(s) 231
Eco88I CYCGRG 1 cut(s) 220
EcoRII CCWGG 2 cut(s) 116, 153
FaiI YATR 6 cut(s) 68, 170, 192, 291, 324, 326
FbaI TGATCA 1 cut(s) 99
Fnu4HI GCNGC 2 cut(s) 202, 337
FokI GGATG 1 cut(s) 320
Fsp4HI GCNGC 2 cut(s) 202, 337
FspBI CTAG 1 cut(s) 162
GlaI GCGC 1 cut(s) 231
GluI GCNGC 2 cut(s) 202, 337
HaeII RGCGCY 1 cut(s) 233
HapII CCGG 3 cut(s) 95, 221, 270
HhaI GCGC 1 cut(s) 232
Hin6I GCGC 1 cut(s) 230
HinP1I GCGC 1 cut(s) 230
HinfI GANTC 2 cut(s) 56, 281
HpaII CCGG 3 cut(s) 95, 221, 270
HphI GGTGA 1 cut(s) 109
HpyCH4III ACNGT 1 cut(s) 314
HpyCH4V TGCA 1 cut(s) 74
HspAI GCGC 1 cut(s) 230
Ksp22I TGATCA 1 cut(s) 99
Kzo9I GATC 3 cut(s) 9, 85, 99
Lsp1109I GCAGC 2 cut(s) 213, 348
MaeI CTAG 1 cut(s) 162
MaeIII GTNAC 1 cut(s) 329
MalI GATC 3 cut(s) 11, 87, 101
MboI GATC 3 cut(s) 9, 85, 99
MboII GAAGA 3 cut(s) 19, 65, 259
MflI RGATCY 1 cut(s) 9
MluCI AATT 2 cut(s) 69, 149
MnlI CCTC 2 cut(s) 270, 298
MroXI GAANNNNTTC 1 cut(s) 149
MseI TTAA 1 cut(s) 197
MspCI CTTAAG 1 cut(s) 196
MspI CCGG 3 cut(s) 95, 221, 270
MspR9I CCNGG 4 cut(s) 118, 155, 221, 222
MvaI CCWGG 2 cut(s) 118, 155
NciI CCSGG 2 cut(s) 221, 222
NdeII GATC 3 cut(s) 9, 85, 99
NlaIV GGNNCC 1 cut(s) 159
PdmI GAANNNNTTC 1 cut(s) 149
PfeI GAWTC 2 cut(s) 56, 281
PfoI TCCNGGA 1 cut(s) 116
PkrI GCNGC 2 cut(s) 203, 338
Psp6I CCWGG 2 cut(s) 116, 153
PspGI CCWGG 2 cut(s) 116, 153
PspN4I GGNNCC 1 cut(s) 159
PsuI RGATCY 1 cut(s) 9
SaqAI TTAA 1 cut(s) 197
SatI GCNGC 2 cut(s) 202, 337
Sau3AI GATC 3 cut(s) 9, 85, 99
ScrFI CCNGG 4 cut(s) 118, 155, 221, 222
SetI ASST 3 cut(s) 129, 262, 350
SmaI CCCGGG 1 cut(s) 222
SmlI CTYRAG 1 cut(s) 196
SmoI CTYRAG 1 cut(s) 196
Sse9I AATT 2 cut(s) 69, 149
SspMI CTAG 1 cut(s) 162
StyD4I CCNGG 4 cut(s) 116, 153, 219, 220
TaaI ACNGT 1 cut(s) 314
TaqI TCGA 3 cut(s) 39, 59, 88
TasI AATT 2 cut(s) 69, 149
TfiI GAWTC 2 cut(s) 56, 281
Tru1I TTAA 1 cut(s) 197
Tru9I TTAA 1 cut(s) 197
TseI GCWGC 2 cut(s) 201, 336
TspDTI ATGAA 2 cut(s) 57, 306
TspMI CCCGGG 1 cut(s) 220
Vha464I CTTAAG 1 cut(s) 196
XapI RAATTY 1 cut(s) 149
XmaI CCCGGG 1 cut(s) 220
XmnI GAANNNNTTC 1 cut(s) 149
XspI CTAG 1 cut(s) 162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.