MD16G1144100.v1.1

Belongs to the DHHC palmitoyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0011556
Physical Location & Seq
Reverse (-)
11084468 .. 11088498
4031 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1144100.v1.1.491

Sequence Viewer

Length: 1320 bp
ATGAGAAACAACGACATGGCCAGAACGCAGCACGAAAGAATGCACGCAAATGCTCTGCCTCAGCATTCTGACTCCGACCGTCGGATTATTGACGCCCCTGGCCCGTCCCTGCTGCTTTATCAAGTTTGGAAAGGAAACAATAAATTTTGCTTTAATGGAAGAATTATATTGGGTCCAGATTCGAGGTCATTATTCCTTACTGTGTCCCTAATTGTGGTTCCAGTGATCCTGTTTTGCGCTTTTCTTTCGCAAGGACTAATTCATCAACTCCCGCTCTACACTGGCATTCTTATTGTGGCTTTATGTCCTGTATTAGCAGTATATGTTGTGATTCTTCTCTTGATTACATCTGCAAGAGATCCTGGCATTATTCCTCGTAGCTTGCATCCTCCAGAACCAGAAGATGACGGCTACGTTTCTAGTATCTCTTCTGATTGGCCAGGAAGTCAGAATGGACCTCCAGGCATACCACCCACAAAAGAAGTTATCATTAATGGCGTGATAGTTAAAACCAAATATTGTCAAACATGCATGCTATATCGCCCACCCCGATGCTCTCACTGCTCTATATGCAACAACTGCGTTGAGCGTTTTGATCACCATTGCCCATGGGTGGGGCAATGTATTGGCAAAAGGAATTACAGATTCTTTTTCATGTTTGTGTCCTCCACGACCGTTCTGTGTCTCTATGTCTTTGCGTTCTGCTGGGTAAACATCATGCAGATAATGGATTATTACCATTGCAGTCTCTGGAGAGCTTTATCAAAGTCACCTGTTTCAGGAGTTCTAATTCTCTATACATTTGGAGTTGCTTGGTTTGTTGGAGGTCTCACAGGATTCCATCTCTATTTAATCCTCACTAATCAGACAACATATGAAAACTTTCGGTATCGGTACAATCTGAAGATGAACCCTTACAATCGTGGGTGTTTTAGCAATATTACGGAAGTCTTCTTCTCTGAAATTCCAAGATCTAAACATAACTTCAGGGAAGTGGTCAAGGGCGACACTTCTTCTGTCTACACCACTTCAATGCCATCAGGCCATCCCATGAGCCCAGAGATGCCCAAAGCAAGTTCTGACATAGAAATGGGAAAACGAAAAGCTGTTGCTGCTGAAGATCTGGAGGATATACAAACTCAGATTGATAGTGTTGGTGGATTGCAGCGGTGTGAGATCCAGCCAAGACGTACAAAATGGGATCACAAAGCCAACTGGGGGAGCTCACCTGATATACAAATGTTGGCTGCGGAGTTTGGAATGGAACATGGTTCAACAGGCAGACAGAAAATTGAAGGGAGTACTAATCTGAAGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04391 map04391
Pfam Domains
Protein Families
DHHC

Protein Analysis

440

Amino Acids

49.58

Weight (kDa)

8.62

Isoelectric Point (pI)

43.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DHHC PF01529 170 - 296 1.9e-37 DHHC palmitoyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 274
AccI GTMKAC 1 cut(s) 1020
AciI CCGC 3 cut(s) 272, 1168, 1250
AclWI GGATC 4 cut(s) 220, 353, 1171, 1209
AcoI YGGCCR 2 cut(s) 18, 437
AcsI RAATTY 2 cut(s) 143, 963
AcuI CTGAAG 3 cut(s) 923, 970, 1137
AcyI GRCGYC 1 cut(s) 93
AfaI GTAC 3 cut(s) 896, 1192, 1303
AfiI CCNNNNNNNGG 6 cut(s) 81, 214, 613, 614, 779, 1218
AgsI TTSAA 3 cut(s) 1032, 1275, 1295
AjnI CCWGG 4 cut(s) 97, 361, 439, 460
AluBI AGCT 5 cut(s) 381, 758, 1106, 1224, 1317
AluI AGCT 5 cut(s) 381, 758, 1106, 1224, 1317
Alw21I GWGCWC 1 cut(s) 1226
Alw26I GTCTC 3 cut(s) 689, 752, 833
AlwI GGATC 4 cut(s) 220, 353, 1171, 1209
AlwNI CAGNNNCTG 1 cut(s) 750
AoxI GGCC 4 cut(s) 18, 100, 437, 1042
ApeKI GCWGC 5 cut(s) 28, 112, 1112, 1165, 1247
ApoI RAATTY 2 cut(s) 143, 963
AseI ATTAAT 1 cut(s) 492
Asp700I GAANNNNTTC 1 cut(s) 882
AspLEI GCGC 1 cut(s) 239
AspS9I GGNCC 3 cut(s) 101, 173, 455
AsuHPI GGTGA 3 cut(s) 590, 762, 1218
AvaII GGWCC 2 cut(s) 173, 455
BalI TGGCCA 2 cut(s) 20, 439
BanII GRGCYC 2 cut(s) 1058, 1226
BbsI GAAGAC 1 cut(s) 943
Bbv12I GWGCWC 1 cut(s) 1226
BbvCI CCTCAGC 1 cut(s) 60
BbvI GCAGC 5 cut(s) 40, 99, 1099, 1177, 1234
BccI CCATC 3 cut(s) 849, 1045, 1053
BceAI ACGGC 1 cut(s) 424
BciT130I CCWGG 4 cut(s) 99, 363, 441, 462
BclI TGATCA 1 cut(s) 595
BcoDI GTCTC 3 cut(s) 689, 752, 833
BfaI CTAG 1 cut(s) 420
BglII AGATCT 2 cut(s) 971, 1120
BisI GCNGC 5 cut(s) 29, 113, 1113, 1166, 1248
BlsI GCNGC 5 cut(s) 30, 114, 1114, 1167, 1249
BmcAI AGTACT 1 cut(s) 1303
Bme1390I CCNGG 4 cut(s) 99, 363, 441, 462
Bme18I GGWCC 2 cut(s) 173, 455
BmgT120I GGNCC 3 cut(s) 101, 173, 455
BmiI GGNNCC 2 cut(s) 174, 219
BmrFI CCNGG 4 cut(s) 99, 363, 441, 462
BmrI ACTGGG 1 cut(s) 1225
BmsI GCATC 3 cut(s) 394, 542, 1053
BmuI ACTGGG 1 cut(s) 1225
BpiI GAAGAC 1 cut(s) 943
BpmI CTGGAG 4 cut(s) 375, 444, 772, 1145
Bpu10I CCTNAGC 1 cut(s) 60
BsaHI GRCGYC 1 cut(s) 93
BsaI GGTCTC 1 cut(s) 833
BsaJI CCNNGG 2 cut(s) 97, 608
Bsc4I CCNNNNNNNGG 6 cut(s) 81, 214, 613, 614, 779, 1218
Bse1I ACTGG 3 cut(s) 221, 286, 1220
Bse3DI GCAATG 3 cut(s) 601, 626, 739
BseBI CCWGG 4 cut(s) 99, 363, 441, 462
BseDI CCNNGG 2 cut(s) 97, 608
BseGI GGATG 2 cut(s) 385, 1045
BseLI CCNNNNNNNGG 6 cut(s) 81, 214, 613, 614, 779, 1218
BseMI GCAATG 3 cut(s) 601, 626, 739
BseMII CTCAG 2 cut(s) 74, 1154
BseNI ACTGG 3 cut(s) 221, 286, 1220
BseXI GCAGC 5 cut(s) 40, 99, 1099, 1177, 1234
BseYI CCCAGC 1 cut(s) 705
Bsh1285I CGRYCG 2 cut(s) 79, 675
BshFI GGCC 4 cut(s) 20, 102, 439, 1044
BsiEI CGRYCG 2 cut(s) 79, 675
BsiHKAI GWGCWC 1 cut(s) 1226
BslFI GGGAC 2 cut(s) 91, 190
BslI CCNNNNNNNGG 6 cut(s) 81, 214, 613, 614, 779, 1218
BsmAI GTCTC 3 cut(s) 689, 752, 833
BsmFI GGGAC 2 cut(s) 91, 190
BsmI GAATGC 3 cut(s) 45, 64, 285
BsnI GGCC 4 cut(s) 20, 102, 439, 1044
Bso31I GGTCTC 1 cut(s) 833
Bsp1286I GDGCHC 2 cut(s) 1058, 1226
Bsp143I GATC 7 cut(s) 225, 358, 595, 971, 1120, 1176, 1201
Bsp19I CCATGG 1 cut(s) 608
BspACI CCGC 3 cut(s) 272, 1168, 1250
BspANI GGCC 4 cut(s) 20, 102, 439, 1044
BspCNI CTCAG 2 cut(s) 73, 1153
BspLI GGNNCC 2 cut(s) 174, 219
BspPI GGATC 4 cut(s) 220, 353, 1171, 1209
BspQI GCTCTTC 1 cut(s) 1307
BspTNI GGTCTC 1 cut(s) 833
BsrBI CCGCTC 1 cut(s) 274
BsrDI GCAATG 3 cut(s) 601, 626, 739
BsrI ACTGG 3 cut(s) 221, 286, 1220
BssECI CCNNGG 2 cut(s) 97, 608
BssMI GATC 7 cut(s) 225, 358, 595, 971, 1120, 1176, 1201
BssNI GRCGYC 1 cut(s) 93
BssT1I CCWWGG 1 cut(s) 608
Bst2UI CCWGG 4 cut(s) 99, 363, 441, 462
Bst4CI ACNGT 3 cut(s) 80, 202, 676
Bst6I CTCTTC 2 cut(s) 433, 1307
BstACI GRCGYC 1 cut(s) 93
BstAPI GCANNNNNTGC 1 cut(s) 579
BstC8I GCNNGC 3 cut(s) 45, 383, 533
BstDEI CTNAG 2 cut(s) 60, 1140
BstDSI CCRYGG 1 cut(s) 608
BstENI CCTNNNNNAGG 1 cut(s) 777
BstF5I GGATG 2 cut(s) 385, 1045
BstHHI GCGC 1 cut(s) 239
BstKTI GATC 7 cut(s) 228, 361, 598, 974, 1123, 1179, 1204
BstMAI GTCTC 3 cut(s) 689, 752, 833
BstMBI GATC 7 cut(s) 225, 358, 595, 971, 1120, 1176, 1201
BstMCI CGRYCG 2 cut(s) 79, 675
BstMWI GCNNNNNNNGC 4 cut(s) 561, 570, 579, 1112
BstNI CCWGG 4 cut(s) 99, 363, 441, 462
BstNSI RCATGY 2 cut(s) 531, 535
BstSCI CCNGG 4 cut(s) 97, 361, 439, 460
BstV1I GCAGC 5 cut(s) 40, 99, 1099, 1177, 1234
BstV2I GAAGAC 1 cut(s) 943
BstX2I RGATCY 4 cut(s) 358, 971, 1120, 1176
BstYI RGATCY 4 cut(s) 358, 971, 1120, 1176
BsuRI GGCC 4 cut(s) 20, 102, 439, 1044
BtgI CCRYGG 1 cut(s) 608
BtsCI GGATG 2 cut(s) 385, 1045
BtsI GCAGTG 1 cut(s) 559
BtsIMutI CAGTG 3 cut(s) 228, 279, 559
Cac8I GCNNGC 3 cut(s) 45, 383, 533
CaiI CAGNNNCTG 1 cut(s) 750
CfoI GCGC 1 cut(s) 239
Cfr13I GGNCC 3 cut(s) 101, 173, 455
CseI GACGC 1 cut(s) 101
Csp6I GTAC 3 cut(s) 895, 1191, 1302
CviAII CATG 8 cut(s) 16, 528, 532, 609, 655, 718, 1051, 1268
CviQI GTAC 3 cut(s) 895, 1191, 1302
DdeI CTNAG 2 cut(s) 60, 1140
DpnI GATC 7 cut(s) 227, 360, 597, 973, 1122, 1178, 1203
DpnII GATC 7 cut(s) 225, 358, 595, 971, 1120, 1176, 1201
EaeI YGGCCR 2 cut(s) 18, 437
Eam1104I CTCTTC 2 cut(s) 433, 1307
EarI CTCTTC 2 cut(s) 433, 1307
Ecl136II GAGCTC 1 cut(s) 1224
Eco130I CCWWGG 1 cut(s) 608
Eco24I GRGCYC 2 cut(s) 1058, 1226
Eco31I GGTCTC 1 cut(s) 833
Eco47I GGWCC 2 cut(s) 173, 455
Eco53kI GAGCTC 1 cut(s) 1224
Eco57I CTGAAG 3 cut(s) 923, 970, 1137
EcoICRI GAGCTC 1 cut(s) 1224
EcoNI CCTNNNNNAGG 1 cut(s) 777
EcoRII CCWGG 4 cut(s) 97, 361, 439, 460
EcoT14I CCWWGG 1 cut(s) 608
EcoT22I ATGCAT 1 cut(s) 533
EcoT38I GRGCYC 2 cut(s) 1058, 1226
ErhI CCWWGG 1 cut(s) 608
FaeI CATG 8 cut(s) 19, 531, 535, 612, 658, 721, 1054, 1271
FaqI GGGAC 2 cut(s) 91, 190
FatI CATG 8 cut(s) 15, 527, 531, 608, 654, 717, 1050, 1267
FauI CCCGC 1 cut(s) 279
FauNDI CATATG 1 cut(s) 874
FbaI TGATCA 1 cut(s) 595
FblI GTMKAC 1 cut(s) 1020
Fnu4HI GCNGC 5 cut(s) 29, 113, 1113, 1166, 1248
FokI GGATG 2 cut(s) 372, 1032
FriOI GRGCYC 2 cut(s) 1058, 1226
Fsp4HI GCNGC 5 cut(s) 29, 113, 1113, 1166, 1248
FspBI CTAG 1 cut(s) 420
GlaI GCGC 1 cut(s) 238
GluI GCNGC 5 cut(s) 29, 113, 1113, 1166, 1248
GsaI CCCAGC 1 cut(s) 709
GsuI CTGGAG 4 cut(s) 375, 444, 772, 1145
HaeIII GGCC 4 cut(s) 20, 102, 439, 1044
HgaI GACGC 1 cut(s) 101
HhaI GCGC 1 cut(s) 239
Hin1I GRCGYC 1 cut(s) 93
Hin1II CATG 8 cut(s) 19, 531, 535, 612, 658, 721, 1054, 1271
Hin6I GCGC 1 cut(s) 237
HinP1I GCGC 1 cut(s) 237
HinfI GANTC 5 cut(s) 71, 179, 331, 645, 837
HphI GGTGA 3 cut(s) 590, 762, 1218
Hpy166II GTNNAC 2 cut(s) 712, 1021
Hpy188III TCNNGA 6 cut(s) 176, 340, 392, 751, 780, 1124
Hpy8I GTNNAC 2 cut(s) 712, 1021
Hpy99I CGWCG 1 cut(s) 84
HpyAV CCTTC 1 cut(s) 1289
HpyCH4III ACNGT 3 cut(s) 80, 202, 676
HpyCH4IV ACGT 2 cut(s) 414, 1189
HpyCH4V TGCA 8 cut(s) 43, 353, 385, 531, 573, 721, 744, 1165
HpyF10VI GCNNNNNNNGC 4 cut(s) 561, 570, 579, 1112
HpyF3I CTNAG 2 cut(s) 60, 1140
HpySE526I ACGT 2 cut(s) 414, 1189
Hsp92I GRCGYC 1 cut(s) 93
Hsp92II CATG 8 cut(s) 19, 531, 535, 612, 658, 721, 1054, 1271
HspAI GCGC 1 cut(s) 237
Ksp22I TGATCA 1 cut(s) 595
Kzo9I GATC 7 cut(s) 225, 358, 595, 971, 1120, 1176, 1201
LguI GCTCTTC 1 cut(s) 1307
LmnI GCTCC 1 cut(s) 1221
Lsp1109I GCAGC 5 cut(s) 40, 99, 1099, 1177, 1234
LweI GCATC 3 cut(s) 394, 542, 1053
MaeI CTAG 1 cut(s) 420
MaeII ACGT 2 cut(s) 414, 1189
MaeIII GTNAC 1 cut(s) 768
MalI GATC 7 cut(s) 227, 360, 597, 973, 1122, 1178, 1203
MbiI CCGCTC 1 cut(s) 274
MboI GATC 7 cut(s) 225, 358, 595, 971, 1120, 1176, 1201
MboII GAAGA 9 cut(s) 171, 326, 413, 420, 916, 943, 946, 1005, 1130
MflI RGATCY 4 cut(s) 358, 971, 1120, 1176
MhlI GDGCHC 2 cut(s) 1058, 1226
MlsI TGGCCA 2 cut(s) 20, 439
MluCI AATT 8 cut(s) 143, 162, 210, 258, 637, 789, 963, 1290
MluNI TGGCCA 2 cut(s) 20, 439
MlyI GAGTC 1 cut(s) 65
MmeI TCCRAC 3 cut(s) 62, 99, 802
MnlI CCTC 9 cut(s) 69, 177, 384, 399, 468, 676, 818, 866, 1120
Mox20I TGGCCA 2 cut(s) 20, 439
Mph1103I ATGCAT 1 cut(s) 533
MroXI GAANNNNTTC 1 cut(s) 882
MscI TGGCCA 2 cut(s) 20, 439
MseI TTAA 4 cut(s) 153, 492, 507, 851
MslI CAYNNNNRTG 5 cut(s) 48, 550, 659, 1031, 1088
Msp20I TGGCCA 2 cut(s) 20, 439
MspA1I CMGCKG 1 cut(s) 1168
MspR9I CCNGG 4 cut(s) 99, 363, 441, 462
Mva1269I GAATGC 3 cut(s) 45, 64, 285
MvaI CCWGG 4 cut(s) 99, 363, 441, 462
MwoI GCNNNNNNNGC 4 cut(s) 561, 570, 579, 1112
NcoI CCATGG 1 cut(s) 608
NdeI CATATG 1 cut(s) 874
NdeII GATC 7 cut(s) 225, 358, 595, 971, 1120, 1176, 1201
NlaIII CATG 8 cut(s) 19, 531, 535, 612, 658, 721, 1054, 1271
NlaIV GGNNCC 2 cut(s) 174, 219
NmuCI GTSAC 1 cut(s) 768
NsiI ATGCAT 1 cut(s) 533
NspI RCATGY 2 cut(s) 531, 535
PaeI GCATGC 1 cut(s) 535
PciSI GCTCTTC 1 cut(s) 1307
PcsI WCGNNNNNNNCGW 1 cut(s) 547
PctI GAATGC 3 cut(s) 45, 64, 285
PdmI GAANNNNTTC 1 cut(s) 882
PfeI GAWTC 4 cut(s) 179, 331, 645, 837
PkrI GCNGC 5 cut(s) 30, 114, 1114, 1167, 1249
PleI GAGTC 1 cut(s) 65
PpsI GAGTC 1 cut(s) 65
PshBI ATTAAT 1 cut(s) 492
Psp124BI GAGCTC 1 cut(s) 1226
Psp6I CCWGG 4 cut(s) 97, 361, 439, 460
PspFI CCCAGC 1 cut(s) 705
PspGI CCWGG 4 cut(s) 97, 361, 439, 460
PspN4I GGNNCC 2 cut(s) 174, 219
PspPI GGNCC 3 cut(s) 101, 173, 455
PstNI CAGNNNCTG 1 cut(s) 750
PsuI RGATCY 4 cut(s) 358, 971, 1120, 1176
RsaI GTAC 3 cut(s) 896, 1192, 1303
RsaNI GTAC 3 cut(s) 895, 1191, 1302
RseI CAYNNNNRTG 5 cut(s) 48, 550, 659, 1031, 1088
SacI GAGCTC 1 cut(s) 1226
SapI GCTCTTC 1 cut(s) 1307
SaqAI TTAA 4 cut(s) 153, 492, 507, 851
SatI GCNGC 5 cut(s) 29, 113, 1113, 1166, 1248
Sau3AI GATC 7 cut(s) 225, 358, 595, 971, 1120, 1176, 1201
Sau96I GGNCC 3 cut(s) 101, 173, 455
ScaI AGTACT 1 cut(s) 1303
SchI GAGTC 1 cut(s) 65
ScrFI CCNGG 4 cut(s) 99, 363, 441, 462
SduI GDGCHC 2 cut(s) 1058, 1226
SfaNI GCATC 3 cut(s) 394, 542, 1053
SinI GGWCC 2 cut(s) 173, 455
SmiMI CAYNNNNRTG 5 cut(s) 48, 550, 659, 1031, 1088
SphI GCATGC 1 cut(s) 535
Sse9I AATT 8 cut(s) 143, 162, 210, 258, 637, 789, 963, 1290
SsiI CCGC 3 cut(s) 272, 1168, 1250
SspI AATATT 2 cut(s) 518, 940
SspMI CTAG 1 cut(s) 420
SstI GAGCTC 1 cut(s) 1226
StyD4I CCNGG 4 cut(s) 97, 361, 439, 460
StyI CCWWGG 1 cut(s) 608
TaaI ACNGT 3 cut(s) 80, 202, 676
TaiI ACGT 2 cut(s) 417, 1192
TaqI TCGA 1 cut(s) 182
TasI AATT 8 cut(s) 143, 162, 210, 258, 637, 789, 963, 1290
TatI WGTACW 1 cut(s) 1301
TfiI GAWTC 4 cut(s) 179, 331, 645, 837
Tru1I TTAA 4 cut(s) 153, 492, 507, 851
Tru9I TTAA 4 cut(s) 153, 492, 507, 851
TscAI CASTG 3 cut(s) 228, 286, 566
TseFI GTSAC 1 cut(s) 768
TseI GCWGC 5 cut(s) 28, 112, 1112, 1165, 1247
Tsp45I GTSAC 1 cut(s) 768
TspDTI ATGAA 4 cut(s) 251, 643, 891, 923
TspGWI ACGGA 1 cut(s) 959
TspRI CASTG 3 cut(s) 228, 286, 566
VpaK11BI GGWCC 2 cut(s) 173, 455
VspI ATTAAT 1 cut(s) 492
XagI CCTNNNNNAGG 1 cut(s) 777
XapI RAATTY 2 cut(s) 143, 963
XceI RCATGY 2 cut(s) 531, 535
XmiI GTMKAC 1 cut(s) 1020
XmnI GAANNNNTTC 1 cut(s) 882
XspI CTAG 1 cut(s) 420
ZrmI AGTACT 1 cut(s) 1303
Zsp2I ATGCAT 1 cut(s) 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.