MD16G1158800.v1.1
MYB Family

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0016308
Physical Location & Seq
Forward (+)
12730784 .. 12736810
6027 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1158800.v1.1.491

Sequence Viewer

Length: 1128 bp
ATGGGAAGAGCTCCGTGCTGTGAGAAAGTTGGGGTCAAAAGAGGAAGATGGACAGCTGAAGAAGATGAAATCTTAACCAACCACATTAAAGCTCATGGAGAAGGCTCTTGGAGATCTTTACCCAAGAATGCAGGATTGCTGAGGTGTGGGAAGAGTTGCAGATTGAGGTGGATAAATTATCTGAGGGCTGACTTAAAGAGAGGGAACATCACTTCTGAGGAGGAAGAAATCATTGTTAAGTTACACACTGCTTTGGGTAATAGGTGGTCATTGATTGCTGCTCGTTTACCAGGAAGAACAGACAATGAGATCAAAAACTACTGGAACTCTCATTTGAGTCGAAAAATCTACAGCTTTACCAAGATGGGAAACGAATATCTACCCACCATTCTGAATGATGAGAAGAAGATAGCTTCATGCAACAAGCGTCGTAGAGGCGCTCAAGCGAGCAGATCTACCATGAACAACAAGAGCAAGAACAAGAATTTATTGGCCCCATTTAACCCAATAAAATCAGAAGCAAAGCCTCAACTTGAAAAACCTAGTCCACCAACGGGCACCACTATTAATCGAGGGCAAGTGGGTGACGAGGACAGCAAATTAATGGTTTTTGAGTCTTGGGACCCAAGAAAGAATGGAGATGGTGGGGGAATAGGAATGTTGGGGATGCAAAGTTCTTGTTTGGACAGTGCAGAAATGATTAATTGGAATCCTTGTGGTGAGCAGAAAGGTGTACAATTATGCCTTGGGAATAAAGAAAAGAATATTACAACTAGTTCAACTGCAATACGTGGTGATAATAGTGGTGAAAAGGAGGTTTTGGGGCCATATTGTGAATGTGTAGACATTATGGGCCTTAATAATAATATTTTTGAAAGTGAGGGGAGAAAAAGTACAAGCAATTGGAGCTCATCAAATGCAGCTGAGAGTGGCGGTGGCAGTGGCGGTGGTGGTGGAGAGTTGTTCTCTTTCTCTTCATCAATGAATTCAGGCTTTGATGATGAATGGCTTAACTGGGGTTGGGCAGGTGGTGACCTTCAGTGTCACAATGGTGAACTGGAAGGGTGGGATGAAGGGGAGAGGATAGCTTGGTTGTGGGAAAGTGGCAGTACTGGTGAAGGACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DUF3326 DUF4283 Myb_DNA-binding

Protein Analysis

376

Amino Acids

41.27

Weight (kDa)

7.0

Isoelectric Point (pI)

53.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 14 - 61 8.6e-15 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 17 - 75 2.8e-08 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 67 - 112 2.7e-16 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 71 - 117 1.3e-08 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016308)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g19310
malus_domestica MD13G1158000.v1.1 MD16G1158800.v1.1
prunus_persica Prupe.1G125300_v2.0.a1
pyrus_communis pycom13g13510
rosa_chinensis RchiOBHm_Chr4g0424331
rosa_laevigata RLG00000007485
rosa_multiflora Rmu_sc0002260.1_g000004
rosa_roxburghii Rroxscaffold_5G00366280
rosa_rugosa Rorug04G0195900
rosa_samantha Rh4AG252800 Rh4BG258200 Rh4CG269100 Rh4DG252600
rosa_wichuraiana Rw4G021800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1016
Acc36I ACCTGC 1 cut(s) 1016
AccB1I GGYRCC 1 cut(s) 557
AccI GTMKAC 1 cut(s) 843
AciI CCGC 2 cut(s) 933, 945
AcsI RAATTY 2 cut(s) 484, 985
AcuI CTGAAG 2 cut(s) 78, 1022
AfaI GTAC 3 cut(s) 735, 895, 1111
AfiI CCNNNNNNNGG 1 cut(s) 554
AgsI TTSAA 3 cut(s) 536, 780, 875
AhlI ACTAGT 1 cut(s) 773
AjnI CCWGG 1 cut(s) 289
AleI CACNNNNGTG 1 cut(s) 1050
AloI GAACNNNNNNTCC 2 cut(s) 658, 690
AluBI AGCT 8 cut(s) 11, 56, 92, 354, 413, 909, 923, 1088
AluI AGCT 8 cut(s) 11, 56, 92, 354, 413, 909, 923, 1088
Alw21I GWGCWC 2 cut(s) 13, 911
AoxI GGCC 3 cut(s) 492, 824, 853
ApeKI GCWGC 2 cut(s) 278, 920
ApoI RAATTY 2 cut(s) 484, 985
AseI ATTAAT 3 cut(s) 567, 602, 702
AspLEI GCGC 1 cut(s) 440
AspS9I GGNCC 4 cut(s) 493, 622, 824, 853
AsuHPI GGTGA 7 cut(s) 596, 731, 806, 818, 1043, 1064, 1127
AvaII GGWCC 1 cut(s) 622
BaeGI GKGCMC 1 cut(s) 560
BanI GGYRCC 1 cut(s) 557
BanII GRGCYC 2 cut(s) 13, 911
Bbv12I GWGCWC 2 cut(s) 13, 911
BbvCI CCTCAGC 1 cut(s) 140
BbvI GCAGC 2 cut(s) 265, 932
BccI CCATC 3 cut(s) 42, 358, 635
BciT130I CCWGG 1 cut(s) 291
BcuI ACTAGT 1 cut(s) 773
BfaI CTAG 2 cut(s) 543, 774
BfmI CTRYAG 1 cut(s) 349
BfoI RGCGCY 1 cut(s) 441
BfuAI ACCTGC 1 cut(s) 1016
BglII AGATCT 2 cut(s) 113, 452
BisI GCNGC 2 cut(s) 279, 921
BlsI GCNGC 2 cut(s) 280, 922
BmcAI AGTACT 1 cut(s) 1111
Bme1390I CCNGG 1 cut(s) 291
Bme18I GGWCC 1 cut(s) 622
BmgT120I GGNCC 4 cut(s) 493, 622, 824, 853
BmiI GGNNCC 5 cut(s) 495, 559, 623, 624, 825
BmrFI CCNGG 1 cut(s) 291
BmrI ACTGGG 1 cut(s) 1024
BmsI GCATC 1 cut(s) 657
BmuI ACTGGG 1 cut(s) 1024
BplI GAGNNNNNCTC 2 cut(s) 950, 982
Bpu10I CCTNAGC 1 cut(s) 140
BpuEI CTTGAG 1 cut(s) 426
BsaAI YACGTR 1 cut(s) 791
BsaJI CCNNGG 1 cut(s) 745
BsaXI ACNNNNNCTCC 2 cut(s) 877, 907
Bsc4I CCNNNNNNNGG 1 cut(s) 554
Bse1I ACTGG 4 cut(s) 326, 1019, 1062, 1117
BseBI CCWGG 1 cut(s) 291
BseDI CCNNGG 1 cut(s) 745
BseGI GGATG 2 cut(s) 672, 1075
BseLI CCNNNNNNNGG 1 cut(s) 554
BseMII CTCAG 4 cut(s) 131, 173, 207, 915
BseNI ACTGG 4 cut(s) 326, 1019, 1062, 1117
BseRI GAGGAG 1 cut(s) 233
BseSI GKGCMC 1 cut(s) 560
BseXI GCAGC 2 cut(s) 265, 932
BsgI GTGCAG 1 cut(s) 711
BshFI GGCC 3 cut(s) 494, 826, 855
BshNI GGYRCC 1 cut(s) 557
BsiHKAI GWGCWC 2 cut(s) 13, 911
BslFI GGGAC 1 cut(s) 635
BslI CCNNNNNNNGG 1 cut(s) 554
BsmFI GGGAC 1 cut(s) 635
BsmI GAATGC 1 cut(s) 133
BsnI GGCC 3 cut(s) 494, 826, 855
Bsp1286I GDGCHC 3 cut(s) 13, 560, 911
Bsp1407I TGTACA 1 cut(s) 733
Bsp143I GATC 3 cut(s) 113, 309, 452
BspACI CCGC 2 cut(s) 933, 945
BspANI GGCC 3 cut(s) 494, 826, 855
BspCNI CTCAG 4 cut(s) 132, 174, 208, 916
BspLI GGNNCC 5 cut(s) 495, 559, 623, 624, 825
BspMI ACCTGC 1 cut(s) 1016
BspT107I GGYRCC 1 cut(s) 557
BsrGI TGTACA 1 cut(s) 733
BsrI ACTGG 4 cut(s) 326, 1019, 1062, 1117
BssECI CCNNGG 1 cut(s) 745
BssMI GATC 3 cut(s) 113, 309, 452
BssT1I CCWWGG 1 cut(s) 745
Bst2UI CCWGG 1 cut(s) 291
Bst4CI ACNGT 1 cut(s) 689
Bst6I CTCTTC 2 cut(s) 146, 979
BstAUI TGTACA 1 cut(s) 733
BstBAI YACGTR 1 cut(s) 791
BstC8I GCNNGC 1 cut(s) 448
BstDEI CTNAG 4 cut(s) 140, 182, 216, 924
BstEII GGTNACC 1 cut(s) 1031
BstF5I GGATG 2 cut(s) 672, 1075
BstH2I RGCGCY 1 cut(s) 441
BstHHI GCGC 1 cut(s) 440
BstKTI GATC 3 cut(s) 116, 312, 455
BstMBI GATC 3 cut(s) 113, 309, 452
BstMWI GCNNNNNNNGC 1 cut(s) 906
BstNI CCWGG 1 cut(s) 291
BstPI GGTNACC 1 cut(s) 1031
BstSCI CCNGG 1 cut(s) 289
BstSFI CTRYAG 1 cut(s) 349
BstSLI GKGCMC 1 cut(s) 560
BstV1I GCAGC 2 cut(s) 265, 932
BstX2I RGATCY 2 cut(s) 113, 452
BstYI RGATCY 2 cut(s) 113, 452
BsuRI GGCC 3 cut(s) 494, 826, 855
BtsCI GGATG 2 cut(s) 672, 1075
BtsI GCAGTG 2 cut(s) 246, 946
BtsIMutI CAGTG 4 cut(s) 246, 694, 946, 1046
BveI ACCTGC 1 cut(s) 1016
Cac8I GCNNGC 1 cut(s) 448
CfoI GCGC 1 cut(s) 440
Cfr13I GGNCC 4 cut(s) 493, 622, 824, 853
CseI GACGC 1 cut(s) 416
Csp6I GTAC 3 cut(s) 734, 894, 1110
CviAII CATG 3 cut(s) 95, 417, 460
CviQI GTAC 3 cut(s) 734, 894, 1110
DdeI CTNAG 4 cut(s) 140, 182, 216, 924
DpnI GATC 3 cut(s) 115, 311, 454
DpnII GATC 3 cut(s) 113, 309, 452
Eam1104I CTCTTC 2 cut(s) 146, 979
EarI CTCTTC 2 cut(s) 146, 979
Ecl136II GAGCTC 2 cut(s) 11, 909
Eco130I CCWWGG 1 cut(s) 745
Eco24I GRGCYC 2 cut(s) 13, 911
Eco47I GGWCC 1 cut(s) 622
Eco53kI GAGCTC 2 cut(s) 11, 909
Eco57I CTGAAG 2 cut(s) 78, 1022
Eco91I GGTNACC 1 cut(s) 1031
EcoICRI GAGCTC 2 cut(s) 11, 909
EcoO109I RGGNCCY 1 cut(s) 622
EcoO65I GGTNACC 1 cut(s) 1031
EcoRI GAATTC 1 cut(s) 985
EcoRII CCWGG 1 cut(s) 289
EcoT14I CCWWGG 1 cut(s) 745
EcoT38I GRGCYC 2 cut(s) 13, 911
ErhI CCWWGG 1 cut(s) 745
FaeI CATG 3 cut(s) 98, 420, 463
FaiI YATR 6 cut(s) 96, 418, 461, 742, 829, 851
FaqI GGGAC 1 cut(s) 635
FatI CATG 3 cut(s) 94, 416, 459
FblI GTMKAC 1 cut(s) 843
Fnu4HI GCNGC 2 cut(s) 279, 921
FokI GGATG 2 cut(s) 679, 1082
FriOI GRGCYC 2 cut(s) 13, 911
Fsp4HI GCNGC 2 cut(s) 279, 921
FspBI CTAG 2 cut(s) 543, 774
GlaI GCGC 1 cut(s) 439
GluI GCNGC 2 cut(s) 279, 921
HaeII RGCGCY 1 cut(s) 441
HaeIII GGCC 3 cut(s) 494, 826, 855
HgaI GACGC 1 cut(s) 416
HhaI GCGC 1 cut(s) 440
Hin1II CATG 3 cut(s) 98, 420, 463
Hin6I GCGC 1 cut(s) 438
HinP1I GCGC 1 cut(s) 438
HinfI GANTC 3 cut(s) 337, 614, 709
HphI GGTGA 7 cut(s) 596, 731, 806, 818, 1043, 1064, 1127
Hpy166II GTNNAC 5 cut(s) 287, 548, 734, 844, 1055
Hpy188I TCNGA 4 cut(s) 183, 217, 393, 517
Hpy8I GTNNAC 5 cut(s) 287, 548, 734, 844, 1055
Hpy99I CGWCG 1 cut(s) 432
HpyAV CCTTC 5 cut(s) 95, 1046, 1055, 1067, 1112
HpyCH4III ACNGT 1 cut(s) 689
HpyCH4IV ACGT 1 cut(s) 790
HpyCH4V TGCA 7 cut(s) 131, 159, 420, 670, 692, 785, 920
HpyF10VI GCNNNNNNNGC 1 cut(s) 906
HpyF3I CTNAG 4 cut(s) 140, 182, 216, 924
HpySE526I ACGT 1 cut(s) 790
Hsp92II CATG 3 cut(s) 98, 420, 463
HspAI GCGC 1 cut(s) 438
KflI GGGWCCC 1 cut(s) 622
Kzo9I GATC 3 cut(s) 113, 309, 452
LmnI GCTCC 2 cut(s) 16, 906
LpnPI CCDG 9 cut(s) 117, 276, 303, 307, 975, 1000, 1011, 1043, 1098
Lsp1109I GCAGC 2 cut(s) 265, 932
LweI GCATC 1 cut(s) 657
MaeI CTAG 2 cut(s) 543, 774
MaeII ACGT 1 cut(s) 790
MaeIII GTNAC 4 cut(s) 240, 584, 1031, 1043
MalI GATC 3 cut(s) 115, 311, 454
MboI GATC 3 cut(s) 113, 309, 452
MfeI CAATTG 1 cut(s) 901
MflI RGATCY 2 cut(s) 113, 452
MhlI GDGCHC 3 cut(s) 13, 560, 911
MluCI AATT 7 cut(s) 175, 484, 599, 703, 737, 901, 985
MlyI GAGTC 2 cut(s) 346, 623
MslI CAYNNNNRTG 1 cut(s) 1050
MspA1I CMGCKG 2 cut(s) 56, 923
MspR9I CCNGG 1 cut(s) 291
MunI CAATTG 1 cut(s) 901
Mva1269I GAATGC 1 cut(s) 133
MvaI CCWGG 1 cut(s) 291
MwoI GCNNNNNNNGC 1 cut(s) 906
NdeII GATC 3 cut(s) 113, 309, 452
NlaIII CATG 3 cut(s) 98, 420, 463
NlaIV GGNNCC 5 cut(s) 495, 559, 623, 624, 825
NmuCI GTSAC 3 cut(s) 584, 1031, 1043
OliI CACNNNNGTG 1 cut(s) 1050
PaqCI CACCTGC 1 cut(s) 1016
PctI GAATGC 1 cut(s) 133
PfeI GAWTC 1 cut(s) 709
PkrI GCNGC 2 cut(s) 280, 922
PleI GAGTC 2 cut(s) 345, 622
PpsI GAGTC 2 cut(s) 345, 622
Ppu21I YACGTR 1 cut(s) 791
PpuMI RGGWCCY 1 cut(s) 622
PshBI ATTAAT 3 cut(s) 567, 602, 702
Psp124BI GAGCTC 2 cut(s) 13, 911
Psp5II RGGWCCY 1 cut(s) 622
Psp6I CCWGG 1 cut(s) 289
PspEI GGTNACC 1 cut(s) 1031
PspGI CCWGG 1 cut(s) 289
PspN4I GGNNCC 5 cut(s) 495, 559, 623, 624, 825
PspPI GGNCC 4 cut(s) 493, 622, 824, 853
PspPPI RGGWCCY 1 cut(s) 622
PsuI RGATCY 2 cut(s) 113, 452
PvuII CAGCTG 2 cut(s) 56, 923
RsaI GTAC 3 cut(s) 735, 895, 1111
RsaNI GTAC 3 cut(s) 734, 894, 1110
RseI CAYNNNNRTG 1 cut(s) 1050
SacI GAGCTC 2 cut(s) 13, 911
SatI GCNGC 2 cut(s) 279, 921
Sau3AI GATC 3 cut(s) 113, 309, 452
Sau96I GGNCC 4 cut(s) 493, 622, 824, 853
ScaI AGTACT 1 cut(s) 1111
SchI GAGTC 2 cut(s) 346, 623
ScrFI CCNGG 1 cut(s) 291
SduI GDGCHC 3 cut(s) 13, 560, 911
SfaNI GCATC 1 cut(s) 657
SfcI CTRYAG 1 cut(s) 349
SinI GGWCC 1 cut(s) 622
SmiMI CAYNNNNRTG 1 cut(s) 1050
SmlI CTYRAG 1 cut(s) 441
SmoI CTYRAG 1 cut(s) 441
SpeI ACTAGT 1 cut(s) 773
Sse9I AATT 7 cut(s) 175, 484, 599, 703, 737, 901, 985
SsiI CCGC 2 cut(s) 933, 945
SspI AATATT 2 cut(s) 766, 868
SspMI CTAG 2 cut(s) 543, 774
SstI GAGCTC 2 cut(s) 13, 911
StyD4I CCNGG 1 cut(s) 289
StyI CCWWGG 1 cut(s) 745
TaaI ACNGT 1 cut(s) 689
TaiI ACGT 1 cut(s) 793
TaqI TCGA 2 cut(s) 340, 571
TasI AATT 7 cut(s) 175, 484, 599, 703, 737, 901, 985
TatI WGTACW 3 cut(s) 733, 893, 1109
TfiI GAWTC 1 cut(s) 709
TscAI CASTG 4 cut(s) 253, 694, 946, 1046
TseFI GTSAC 3 cut(s) 584, 1031, 1043
TseI GCWGC 2 cut(s) 278, 920
Tsp45I GTSAC 3 cut(s) 584, 1031, 1043
TspDTI ATGAA 7 cut(s) 81, 405, 476, 966, 998, 1017, 1086
TspRI CASTG 4 cut(s) 253, 694, 946, 1046
VpaK11BI GGWCC 1 cut(s) 622
VspI ATTAAT 3 cut(s) 567, 602, 702
XapI RAATTY 2 cut(s) 484, 985
XmiI GTMKAC 1 cut(s) 843
XspI CTAG 2 cut(s) 543, 774
ZrmI AGTACT 1 cut(s) 1111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.