MD16G1173400.v1.1

acyl-CoA-binding domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0010980
Physical Location & Seq
Reverse (-)
14628802 .. 14634092
5291 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1173400.v1.1.491

Sequence Viewer

Length: 2148 bp
ATGTTCGGTTTTTCTCGCAGGCGCATGAAGCTTGGACGGTTGAAAGTCCAGCTTTCAGATTCTGCTCAGGGAACTAGAAGCCCCATAAGACACCCGAAACGAAATAACAGCTCCAATAATGAATGTGATGTGCCGGCAACTAGTAATTCCGATGAGCTCGATAGCCAGTGTCTATCTGCTGTGCCCGAGATTAGTAATTCCGCCACTGGGAGCTCTGAGAATTGGATGGTGTTGTTGATTTCTGGGGACCAGCCTACGCCCCGCTTCAATCATGCTGCTGCTGTAATTGGGAACAAGATGATAGTTGTTGGTGGTGAATCTGGGAATGAATTGCTAGATGATGTACAGGTGCTTAATTTTGATAGTTTTACGTGGACCACTGCTTCGTCAAAGCTTTATTTGTCACCGAGCAGTCTGCCATTAAAGATCCCTGCATGCAAGGGCCACAAACTGGTGTCTTGGGGGAAAAAGGCACTCCTCATTGGAGGAAAAACTGAACCGGGGAGCGACAAAATTTCAGTTTGGGCATTTGATACAGAGACAGAATGCTGGTCTCATATGGAAGCAAAGGGCGATATACCGGTTGCTCGCACTGGTCACACTGTAGTCAGGGCAAGCTCTACATTAATCCTATTTGGAGGTGAAGATGCTAAAAGGAGGAAATTGAACGATTTGCATATGTTTGATCTGAAGTCGTTGACATGGTTACCCCTTCATTGCACCGGTACAGGACCATCTGCAAGGTCCAACCACGTAGCAGCTCTTTATGATGATAAAACTCTTTTTATATTTGGAGGCACATCAAAATCCAAGACTTTGAATGATTTGTATTCAATGGACTTTGAAACTATGGAGTGGTCAAGAATAAAGAAACGAGGTTTCCATCCATCTCCTAGGGCTGGTTGCTGTGGGGTCCTATGTGGAACCAAATGGTATATTGTTGGGGGTGGAAGCAGGAAAAAACGACATGCTGAGACTCTGATCTTTGATATCTTGAAACTTGAGTGGTCGGTGGCCATTGCATCGCCTCCTTCTTCAATCACCGCCAATAAGGGTTTCAGCCTAGAGCTCGTGCAACACAAAGAAAAGGATTTTCTTGTTTCTTTTGGGGGATGCAGAAAGGAACCAACGAATCAGGTTGAAGTACTGACCATGGAAAAGAATGAATCATCCATAAGTCGTCGATCTTTTCCCAGCAAAGGCCCTGGAACTTTGCTGCCTGAGAAACGCGCATCATCCACAGGCTTGGCTTCTCAACTAAGTAATGCTTCTTCCCAACGCTCGGTTAATTCGGTTACCAGACAAAATCTTGCATCTGCAATTGAACAACATGGTTCTGGAAGAAAATCTTTGTCAGAGTCCTTGCTTGTTGATCCGAATCCTGTTTCTGGCAACGTTTCACTTAGGAAACAATTCCACAATGAAGAAGAATTCAACACGACTGTTAAGGTGGCAAAAAGTTCAGAAGATGAAAGTTCTTTCTCGCAGCCAATTGAAAAGAAAACCAGTCAATCTGATTCAGGAGTCCAGACTAACATATATGGAGGCAGACTCTTTTCGGAGGAGATACTATCTGGATATGATTTTGAAAATTCAAATCCTCACCTTCATGGAACTGGAAACCTTTCAATGGATAACAACGATGCCGTGTTCCCAGAGAATGATAGTAAACTAGGAGCCCTATCAACTGCTTCAAGTATGTACCACTACTATGAATCGAAGATGGCCGCACTAATAAGAAAGAATGGAATTTTAGAAGGAAAGTTAGCTACTGTGATGGCCAGCCAGGAAGCTGCTGAGAAAAATTTATCCTCGGCCCTCAAGAGCAGACAGGAGATGGAGAGAAAATTGTCAGACACTGCGAAGGAGATGGAATTTCTGAAGGAAAAGGTTGCGGGTGTAGAGATAGCGCAAGAAGAGGCTAACAACCTCTCAAATATTGTTCATTCTGACAACGTCAGGCTTGAGCATGATGTGGCTTTCCTCAAGGCGGTTTTGGATGATACCCAGAAGGAGCTCCATTCAACTAGAGGAGTCCTTGCAGGAGAACGAGCAAGGGCATTCCAACTACAGGTTGAAGTTTTCCACCTGAAACAGAGATTGCAATCTATGGAGAACCGAGCACCGACACCTCGGAAACCTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_1 Kelch_4 Kelch_5

Protein Analysis

716

Amino Acids

78.59

Weight (kDa)

8.62

Isoelectric Point (pI)

48.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_ATRN-LZTR1 PF24981 71 - 164 1.4e-06 Attractin/LZTR1 beta-propeller
Kelch_KLHDC2_KLHL20_DRC7 PF24681 72 - 129 1.8e-11 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 72 - 131 2.1e-10 FBX42, beta-propeller domain
Kelch_HCF PF13854 77 - 318 7.6e-26 Host cell factor, Kelch-repeats domain
Kelch_1 PF01344 87 - 130 7.6e-09 Kelch motif
Beta-prop_FBX42 PF13415 146 - 269 6.1e-24 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 148 - 371 1e-60 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_TYW4 PF13418 149 - 371 3.3e-13 tRNA wybutosine-synthesizing protein 4, beta-propeller
Beta-prop_ATRN-LZTR1 PF24981 160 - 294 8.1e-22 Attractin/LZTR1 beta-propeller
Beta-prop_FBX42 PF13415 254 - 391 5.5e-18 FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 261 - 393 8.9e-12 Attractin/LZTR1 beta-propeller
ACBP4_C PF24922 646 - 705 1.6e-09 Acyl-CoA-binding domain-containing protein 4-6, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010980)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18590 AT5G18590
fragaria_vesca FvH4_4g17510 FvH4_4g17510 FvH4_4g17510
malus_domestica MD16G1173400.v1.1
prunus_persica Prupe.1G140300_v2.0.a1 Prupe.1G140300_v2.0.a1
pyrus_communis pycom16g14540
rosa_chinensis RchiOBHm_Chr4g0421431
rosa_laevigata RLG00000007685
rosa_multiflora Rmu_sc0001962.1_g000012
rosa_roxburghii Rroxscaffold_5G00363700
rosa_rugosa Rorug04G0171000 Rorug04G0171100
rosa_samantha Rh4AG231800 Rh4BG234700 Rh4CG246300 Rh4DG230300
rosa_wichuraiana Rw4G020230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 451
AccII CGCG 1 cut(s) 1230
AciI CCGC 6 cut(s) 201, 262, 1044, 1728, 1895, 1991
AclI AACGTT 1 cut(s) 1395
AclWI GGATC 2 cut(s) 421, 1367
AcoI YGGCCR 3 cut(s) 1014, 1725, 1779
AcsI RAATTY 6 cut(s) 513, 1430, 1591, 1749, 1804, 1874
AcuI CTGAAG 2 cut(s) 710, 1901
AfaI GTAC 4 cut(s) 345, 727, 1146, 1703
AfiI CCNNNNNNNGG 9 cut(s) 207, 451, 899, 1199, 1282, 1388, 1630, 1990, 2071
AgeI ACCGGT 2 cut(s) 580, 722
AhlI ACTAGT 1 cut(s) 140
AjnI CCWGG 2 cut(s) 1204, 1785
AjuI GAANNNNNNNTTGG 2 cut(s) 1040, 1072
Alw21I GWGCWC 5 cut(s) 159, 215, 1071, 2019, 2125
Alw26I GTCTC 3 cut(s) 533, 558, 968
AlwI GGATC 2 cut(s) 421, 1367
AlwNI CAGNNNCTG 2 cut(s) 62, 1859
Ama87I CYCGRG 1 cut(s) 185
AoxI GGCC 6 cut(s) 442, 1014, 1201, 1725, 1779, 1815
ApeKI GCWGC 6 cut(s) 275, 278, 758, 1216, 1486, 1793
ApoI RAATTY 6 cut(s) 513, 1430, 1591, 1749, 1804, 1874
ArsI GACNNNNNNTTYG 2 cut(s) 367, 399
AseI ATTAAT 1 cut(s) 626
AsiGI ACCGGT 2 cut(s) 580, 722
Asp700I GAANNNNTTC 3 cut(s) 1412, 1624, 2141
AspA2I CCTAGG 1 cut(s) 893
AspLEI GCGC 3 cut(s) 24, 1232, 1912
AspS9I GGNCC 8 cut(s) 247, 375, 442, 731, 744, 913, 1202, 1816
AsuC2I CCSGG 1 cut(s) 501
AsuHPI GGTGA 5 cut(s) 326, 396, 653, 1033, 1595
AvaI CYCGRG 1 cut(s) 185
AvaII GGWCC 5 cut(s) 247, 375, 731, 744, 913
AvrII CCTAGG 1 cut(s) 893
BaeGI GKGCMC 1 cut(s) 186
BalI TGGCCA 2 cut(s) 1016, 1781
BanII GRGCYC 5 cut(s) 159, 215, 1071, 1681, 2019
BarI GAAGNNNNNNTAC 2 cut(s) 1255, 1287
BauI CACGAG 1 cut(s) 1070
Bbv12I GWGCWC 5 cut(s) 159, 215, 1071, 2019, 2125
BbvI GCAGC 6 cut(s) 262, 265, 770, 1203, 1498, 1780
BccI CCATC 8 cut(s) 220, 742, 891, 895, 1717, 1771, 1831, 1864
BceAI ACGGC 1 cut(s) 1631
BcgI CGANNNNNNTGC 2 cut(s) 397, 431
BciT130I CCWGG 2 cut(s) 1206, 1787
BcnI CCSGG 1 cut(s) 501
BcoDI GTCTC 3 cut(s) 533, 558, 968
BcuI ACTAGT 1 cut(s) 140
BfaI CTAG 8 cut(s) 75, 141, 335, 894, 1064, 1673, 2028, 2146
BfmI CTRYAG 2 cut(s) 603, 2069
BisI GCNGC 7 cut(s) 276, 279, 759, 1217, 1487, 1728, 1794
BlnI CCTAGG 1 cut(s) 893
BlsI GCNGC 7 cut(s) 277, 280, 760, 1218, 1488, 1729, 1795
BmcAI AGTACT 1 cut(s) 1146
Bme1390I CCNGG 3 cut(s) 501, 1206, 1787
Bme18I GGWCC 5 cut(s) 247, 375, 731, 744, 913
BmeT110I CYCGRG 1 cut(s) 185
BmgT120I GGNCC 8 cut(s) 247, 375, 442, 731, 744, 913, 1202, 1816
BmiI GGNNCC 5 cut(s) 248, 914, 925, 1125, 1678
BmrFI CCNGG 3 cut(s) 501, 1206, 1787
BmrI ACTGGG 1 cut(s) 216
BmsI GCATC 6 cut(s) 637, 1031, 1103, 1241, 1322, 1633
BmuI ACTGGG 1 cut(s) 216
BplI GAGNNNNNCTC 2 cut(s) 1536, 1568
Bpu10I CCTNAGC 1 cut(s) 66
BpuEI CTTGAG 4 cut(s) 1022, 1805, 1970, 1985
BpuMI CCSGG 1 cut(s) 501
BsaAI YACGTR 2 cut(s) 372, 754
BsaBI GATNNNNATC 2 cut(s) 1377, 2104
BsaI GGTCTC 1 cut(s) 558
BsaJI CCNNGG 6 cut(s) 500, 893, 1152, 1204, 1812, 2132
BsaWI WCCGGW 2 cut(s) 580, 722
Bsc4I CCNNNNNNNGG 9 cut(s) 207, 451, 899, 1199, 1282, 1388, 1630, 1990, 2071
Bse118I RCCGGY 3 cut(s) 133, 580, 722
Bse1I ACTGG 6 cut(s) 166, 211, 456, 598, 1506, 1621
Bse3DI GCAATG 2 cut(s) 715, 1017
Bse8I GATNNNNATC 2 cut(s) 1377, 2104
BseBI CCWGG 2 cut(s) 1206, 1787
BseDI CCNNGG 6 cut(s) 500, 893, 1152, 1204, 1812, 2132
BseGI GGATG 6 cut(s) 231, 883, 1118, 1169, 1235, 2005
BseJI GATNNNNATC 2 cut(s) 1377, 2104
BseLI CCNNNNNNNGG 9 cut(s) 207, 451, 899, 1199, 1282, 1388, 1630, 1990, 2071
BseMI GCAATG 2 cut(s) 715, 1017
BseMII CTCAG 5 cut(s) 80, 207, 963, 1212, 1788
BseNI ACTGG 6 cut(s) 166, 211, 456, 598, 1506, 1621
BseRI GAGGAG 3 cut(s) 467, 1577, 2046
BseSI GKGCMC 1 cut(s) 186
BseXI GCAGC 6 cut(s) 262, 265, 770, 1203, 1498, 1780
BseYI CCCAGC 1 cut(s) 1193
Bsh1236I CGCG 1 cut(s) 1230
BshFI GGCC 6 cut(s) 444, 1016, 1203, 1727, 1781, 1817
BshTI ACCGGT 2 cut(s) 580, 722
BsiHKAI GWGCWC 5 cut(s) 159, 215, 1071, 2019, 2125
BsiHKCI CYCGRG 1 cut(s) 185
BsiSI CCGG 4 cut(s) 134, 500, 581, 723
BslFI GGGAC 1 cut(s) 260
BslI CCNNNNNNNGG 9 cut(s) 207, 451, 899, 1199, 1282, 1388, 1630, 1990, 2071
BsmAI GTCTC 3 cut(s) 533, 558, 968
BsmFI GGGAC 1 cut(s) 260
BsmI GAATGC 2 cut(s) 551, 2060
BsnI GGCC 6 cut(s) 444, 1016, 1203, 1727, 1781, 1817
Bso31I GGTCTC 1 cut(s) 558
BsoBI CYCGRG 1 cut(s) 185
Bsp1286I GDGCHC 7 cut(s) 159, 186, 215, 1071, 1681, 2019, 2125
Bsp1407I TGTACA 1 cut(s) 343
Bsp143I GATC 5 cut(s) 426, 685, 981, 1184, 1372
Bsp19I CCATGG 1 cut(s) 1152
BspACI CCGC 6 cut(s) 201, 262, 1044, 1728, 1895, 1991
BspANI GGCC 6 cut(s) 444, 1016, 1203, 1727, 1781, 1817
BspCNI CTCAG 5 cut(s) 79, 208, 964, 1213, 1789
BspFNI CGCG 1 cut(s) 1230
BspLI GGNNCC 5 cut(s) 248, 914, 925, 1125, 1678
BspPI GGATC 2 cut(s) 421, 1367
BspTNI GGTCTC 1 cut(s) 558
BsrDI GCAATG 2 cut(s) 715, 1017
BsrFI RCCGGY 3 cut(s) 133, 580, 722
BsrGI TGTACA 1 cut(s) 343
BsrI ACTGG 6 cut(s) 166, 211, 456, 598, 1506, 1621
BssAI RCCGGY 3 cut(s) 133, 580, 722
BssECI CCNNGG 6 cut(s) 500, 893, 1152, 1204, 1812, 2132
BssMI GATC 5 cut(s) 426, 685, 981, 1184, 1372
BssSI CACGAG 1 cut(s) 1070
BssT1I CCWWGG 2 cut(s) 893, 1152
Bst2BI CACGAG 1 cut(s) 1070
Bst2UI CCWGG 2 cut(s) 1206, 1787
Bst4CI ACNGT 4 cut(s) 39, 604, 1444, 1774
Bst6I CTCTTC 1 cut(s) 1911
BstAUI TGTACA 1 cut(s) 343
BstBAI YACGTR 2 cut(s) 372, 754
BstC8I GCNNGC 6 cut(s) 20, 135, 436, 589, 616, 1783
BstDEI CTNAG 7 cut(s) 66, 216, 972, 1221, 1259, 1403, 1797
BstDSI CCRYGG 1 cut(s) 1152
BstEII GGTNACC 2 cut(s) 705, 1294
BstF5I GGATG 6 cut(s) 231, 883, 1118, 1169, 1235, 2005
BstFNI CGCG 1 cut(s) 1230
BstHHI GCGC 3 cut(s) 24, 1232, 1912
BstKTI GATC 5 cut(s) 429, 688, 984, 1187, 1375
BstMAI GTCTC 3 cut(s) 533, 558, 968
BstMBI GATC 5 cut(s) 426, 685, 981, 1184, 1372
BstMWI GCNNNNNNNGC 1 cut(s) 28
BstNI CCWGG 2 cut(s) 1206, 1787
BstNSI RCATGY 2 cut(s) 438, 971
BstPI GGTNACC 2 cut(s) 705, 1294
BstSCI CCNGG 3 cut(s) 499, 1204, 1785
BstSFI CTRYAG 2 cut(s) 603, 2069
BstSLI GKGCMC 1 cut(s) 186
BstUI CGCG 1 cut(s) 1230
BstV1I GCAGC 6 cut(s) 262, 265, 770, 1203, 1498, 1780
BstX2I RGATCY 1 cut(s) 426
BstXI CCANNNNNNTGG 1 cut(s) 1246
BstYI RGATCY 1 cut(s) 426
BsuRI GGCC 6 cut(s) 444, 1016, 1203, 1727, 1781, 1817
BtgI CCRYGG 1 cut(s) 1152
BtgZI GCGATG 1 cut(s) 1008
BtsCI GGATG 6 cut(s) 231, 883, 1118, 1169, 1235, 2005
BtsI GCAGTG 2 cut(s) 378, 1857
BtsIMutI CAGTG 6 cut(s) 173, 204, 378, 591, 600, 1857
Cac8I GCNNGC 6 cut(s) 20, 135, 436, 589, 616, 1783
CaiI CAGNNNCTG 2 cut(s) 62, 1859
CfoI GCGC 3 cut(s) 24, 1232, 1912
Cfr10I RCCGGY 3 cut(s) 133, 580, 722
Cfr13I GGNCC 8 cut(s) 247, 375, 442, 731, 744, 913, 1202, 1816
Csp6I GTAC 4 cut(s) 344, 726, 1145, 1702
CspAI ACCGGT 2 cut(s) 580, 722
CviAII CATG 9 cut(s) 25, 272, 435, 702, 968, 1153, 1331, 1610, 1970
CviQI GTAC 4 cut(s) 344, 726, 1145, 1702
DdeI CTNAG 7 cut(s) 66, 216, 972, 1221, 1259, 1403, 1797
DpnI GATC 5 cut(s) 428, 687, 983, 1186, 1374
DpnII GATC 5 cut(s) 426, 685, 981, 1184, 1372
EaeI YGGCCR 3 cut(s) 1014, 1725, 1779
Eam1104I CTCTTC 1 cut(s) 1911
EarI CTCTTC 1 cut(s) 1911
EciI GGCGGA 1 cut(s) 190
Ecl136II GAGCTC 4 cut(s) 157, 213, 1069, 2017
Eco130I CCWWGG 2 cut(s) 893, 1152
Eco24I GRGCYC 5 cut(s) 159, 215, 1071, 1681, 2019
Eco31I GGTCTC 1 cut(s) 558
Eco32I GATATC 1 cut(s) 991
Eco47I GGWCC 5 cut(s) 247, 375, 731, 744, 913
Eco53kI GAGCTC 4 cut(s) 157, 213, 1069, 2017
Eco57I CTGAAG 2 cut(s) 710, 1901
Eco88I CYCGRG 1 cut(s) 185
Eco91I GGTNACC 2 cut(s) 705, 1294
EcoICRI GAGCTC 4 cut(s) 157, 213, 1069, 2017
EcoO109I RGGNCCY 2 cut(s) 913, 1202
EcoO65I GGTNACC 2 cut(s) 705, 1294
EcoRI GAATTC 1 cut(s) 1430
EcoRII CCWGG 2 cut(s) 1204, 1785
EcoRV GATATC 1 cut(s) 991
EcoT14I CCWWGG 2 cut(s) 893, 1152
EcoT38I GRGCYC 5 cut(s) 159, 215, 1071, 1681, 2019
ErhI CCWWGG 2 cut(s) 893, 1152
FaeI CATG 9 cut(s) 28, 275, 438, 705, 971, 1156, 1334, 1613, 1973
FalI AAGNNNNNCTT 6 cut(s) 36, 68, 1252, 1284, 1333, 1365
FaqI GGGAC 1 cut(s) 260
FatI CATG 9 cut(s) 24, 271, 434, 701, 967, 1152, 1330, 1609, 1969
FauI CCCGC 2 cut(s) 269, 1888
FauNDI CATATG 2 cut(s) 558, 678
Fnu4HI GCNGC 7 cut(s) 276, 279, 759, 1217, 1487, 1728, 1794
FokI GGATG 6 cut(s) 238, 870, 1125, 1156, 1222, 2012
FriOI GRGCYC 5 cut(s) 159, 215, 1071, 1681, 2019
Fsp4HI GCNGC 7 cut(s) 276, 279, 759, 1217, 1487, 1728, 1794
FspBI CTAG 8 cut(s) 75, 141, 335, 894, 1064, 1673, 2028, 2146
GlaI GCGC 3 cut(s) 23, 1231, 1911
GluI GCNGC 7 cut(s) 276, 279, 759, 1217, 1487, 1728, 1794
GsaI CCCAGC 1 cut(s) 1197
HaeIII GGCC 6 cut(s) 444, 1016, 1203, 1727, 1781, 1817
HapII CCGG 4 cut(s) 134, 500, 581, 723
HhaI GCGC 3 cut(s) 24, 1232, 1912
Hin1II CATG 9 cut(s) 28, 275, 438, 705, 971, 1156, 1334, 1613, 1973
Hin6I GCGC 3 cut(s) 22, 1230, 1910
HinP1I GCGC 3 cut(s) 22, 1230, 1910
HincII GTYRAC 1 cut(s) 699
HindII GTYRAC 1 cut(s) 699
HindIII AAGCTT 2 cut(s) 29, 392
HpaII CCGG 4 cut(s) 134, 500, 581, 723
HphI GGTGA 5 cut(s) 326, 396, 653, 1033, 1595
Hpy166II GTNNAC 3 cut(s) 375, 699, 1670
Hpy188III TCNNGA 7 cut(s) 861, 994, 1338, 1521, 1528, 1575, 1822
Hpy8I GTNNAC 3 cut(s) 375, 699, 1670
Hpy99I CGWCG 1 cut(s) 1185
HpyAV CCTTC 7 cut(s) 722, 1041, 1616, 1751, 1858, 1876, 2005
HpyCH4III ACNGT 4 cut(s) 39, 604, 1444, 1774
HpyCH4IV ACGT 4 cut(s) 371, 753, 1395, 1956
HpyF10VI GCNNNNNNNGC 1 cut(s) 28
HpyF3I CTNAG 7 cut(s) 66, 216, 972, 1221, 1259, 1403, 1797
HpySE526I ACGT 4 cut(s) 371, 753, 1395, 1956
Hsp92II CATG 9 cut(s) 28, 275, 438, 705, 971, 1156, 1334, 1613, 1973
HspAI GCGC 3 cut(s) 22, 1230, 1910
KroI GCCGGC 1 cut(s) 133
KroNI GCCGGC 1 cut(s) 135
Kzo9I GATC 5 cut(s) 426, 685, 981, 1184, 1372
LmnI GCTCC 6 cut(s) 116, 210, 504, 1676, 2014, 2022
Lsp1109I GCAGC 6 cut(s) 262, 265, 770, 1203, 1498, 1780
LweI GCATC 6 cut(s) 637, 1031, 1103, 1241, 1322, 1633
MaeI CTAG 8 cut(s) 75, 141, 335, 894, 1064, 1673, 2028, 2146
MaeII ACGT 4 cut(s) 371, 753, 1395, 1956
MaeIII GTNAC 4 cut(s) 402, 596, 705, 1294
MalI GATC 5 cut(s) 428, 687, 983, 1186, 1374
MboI GATC 5 cut(s) 426, 685, 981, 1184, 1372
MboII GAAGA 9 cut(s) 656, 1026, 1263, 1353, 1436, 1439, 1478, 1732, 1928
MfeI CAATTG 2 cut(s) 1320, 1491
MflI RGATCY 1 cut(s) 426
MhlI GDGCHC 7 cut(s) 159, 186, 215, 1071, 1681, 2019, 2125
MlsI TGGCCA 2 cut(s) 1016, 1781
MluNI TGGCCA 2 cut(s) 1016, 1781
MlyI GAGTC 5 cut(s) 970, 1367, 1533, 1545, 2043
MmeI TCCRAC 2 cut(s) 771, 2089
Mox20I TGGCCA 2 cut(s) 1016, 1781
MroNI GCCGGC 1 cut(s) 133
MroXI GAANNNNTTC 3 cut(s) 1412, 1624, 2141
MscI TGGCCA 2 cut(s) 1016, 1781
MseI TTAA 5 cut(s) 354, 422, 626, 1287, 1446
MslI CAYNNNNRTG 2 cut(s) 1608, 1710
Msp20I TGGCCA 2 cut(s) 1016, 1781
MspI CCGG 4 cut(s) 134, 500, 581, 723
MspR9I CCNGG 3 cut(s) 501, 1206, 1787
MunI CAATTG 2 cut(s) 1320, 1491
Mva1269I GAATGC 2 cut(s) 551, 2060
MvaI CCWGG 2 cut(s) 1206, 1787
MvnI CGCG 1 cut(s) 1230
MwoI GCNNNNNNNGC 1 cut(s) 28
NaeI GCCGGC 1 cut(s) 135
NciI CCSGG 1 cut(s) 501
NcoI CCATGG 1 cut(s) 1152
NdeI CATATG 2 cut(s) 558, 678
NdeII GATC 5 cut(s) 426, 685, 981, 1184, 1372
NgoMIV GCCGGC 1 cut(s) 133
NlaIII CATG 9 cut(s) 28, 275, 438, 705, 971, 1156, 1334, 1613, 1973
NlaIV GGNNCC 5 cut(s) 248, 914, 925, 1125, 1678
NmeAIII GCCGAG 1 cut(s) 1793
NmuCI GTSAC 2 cut(s) 402, 596
NspI RCATGY 2 cut(s) 438, 971
PaeI GCATGC 1 cut(s) 438
PctI GAATGC 2 cut(s) 551, 2060
PdiI GCCGGC 1 cut(s) 135
PdmI GAANNNNTTC 3 cut(s) 1412, 1624, 2141
PfeI GAWTC 7 cut(s) 59, 317, 1132, 1166, 1378, 1517, 1715
PflFI GACNNNGTC 1 cut(s) 1955
PflMI CCANNNNNTGG 1 cut(s) 451
PinAI ACCGGT 2 cut(s) 580, 722
PkrI GCNGC 7 cut(s) 277, 280, 760, 1218, 1488, 1729, 1795
PleI GAGTC 5 cut(s) 970, 1366, 1532, 1545, 2042
PpsI GAGTC 5 cut(s) 970, 1366, 1532, 1545, 2042
Ppu21I YACGTR 2 cut(s) 372, 754
PpuMI RGGWCCY 1 cut(s) 913
PshBI ATTAAT 1 cut(s) 626
Psp124BI GAGCTC 4 cut(s) 159, 215, 1071, 2019
Psp1406I AACGTT 1 cut(s) 1395
Psp5II RGGWCCY 1 cut(s) 913
Psp6I CCWGG 2 cut(s) 1204, 1785
PspEI GGTNACC 2 cut(s) 705, 1294
PspFI CCCAGC 1 cut(s) 1193
PspGI CCWGG 2 cut(s) 1204, 1785
PspN4I GGNNCC 5 cut(s) 248, 914, 925, 1125, 1678
PspPI GGNCC 8 cut(s) 247, 375, 442, 731, 744, 913, 1202, 1816
PspPPI RGGWCCY 1 cut(s) 913
PstNI CAGNNNCTG 2 cut(s) 62, 1859
PsuI RGATCY 1 cut(s) 426
PsyI GACNNNGTC 1 cut(s) 1955
RsaI GTAC 4 cut(s) 345, 727, 1146, 1703
RsaNI GTAC 4 cut(s) 344, 726, 1145, 1702
RseI CAYNNNNRTG 2 cut(s) 1608, 1710
SacI GAGCTC 4 cut(s) 159, 215, 1071, 2019
SaqAI TTAA 5 cut(s) 354, 422, 626, 1287, 1446
SatI GCNGC 7 cut(s) 276, 279, 759, 1217, 1487, 1728, 1794
Sau3AI GATC 5 cut(s) 426, 685, 981, 1184, 1372
Sau96I GGNCC 8 cut(s) 247, 375, 442, 731, 744, 913, 1202, 1816
ScaI AGTACT 1 cut(s) 1146
SchI GAGTC 5 cut(s) 970, 1367, 1533, 1545, 2043
ScrFI CCNGG 3 cut(s) 501, 1206, 1787
SduI GDGCHC 7 cut(s) 159, 186, 215, 1071, 1681, 2019, 2125
SfaNI GCATC 6 cut(s) 637, 1031, 1103, 1241, 1322, 1633
SfcI CTRYAG 2 cut(s) 603, 2069
SinI GGWCC 5 cut(s) 247, 375, 731, 744, 913
SmiMI CAYNNNNRTG 2 cut(s) 1608, 1710
SmlI CTYRAG 4 cut(s) 1001, 1820, 1964, 1985
SmoI CTYRAG 4 cut(s) 1001, 1820, 1964, 1985
SpeI ACTAGT 1 cut(s) 140
SphI GCATGC 1 cut(s) 438
SsiI CCGC 6 cut(s) 201, 262, 1044, 1728, 1895, 1991
SspI AATATT 1 cut(s) 1939
SspMI CTAG 8 cut(s) 75, 141, 335, 894, 1064, 1673, 2028, 2146
SstI GAGCTC 4 cut(s) 159, 215, 1071, 2019
StyD4I CCNGG 3 cut(s) 499, 1204, 1785
StyI CCWWGG 2 cut(s) 893, 1152
TaaI ACNGT 4 cut(s) 39, 604, 1444, 1774
TaiI ACGT 4 cut(s) 374, 756, 1398, 1959
TaqI TCGA 3 cut(s) 159, 1183, 1718
TatI WGTACW 2 cut(s) 343, 1144
TauI GCSGC 1 cut(s) 1730
TfiI GAWTC 7 cut(s) 59, 317, 1132, 1166, 1378, 1517, 1715
Tru1I TTAA 5 cut(s) 354, 422, 626, 1287, 1446
Tru9I TTAA 5 cut(s) 354, 422, 626, 1287, 1446
TscAI CASTG 6 cut(s) 173, 211, 385, 598, 607, 1864
TseFI GTSAC 2 cut(s) 402, 596
TseI GCWGC 6 cut(s) 275, 278, 758, 1216, 1486, 1793
Tsp45I GTSAC 2 cut(s) 402, 596
TspRI CASTG 6 cut(s) 173, 211, 385, 598, 607, 1864
Tth111I GACNNNGTC 1 cut(s) 1955
Van91I CCANNNNNTGG 1 cut(s) 451
VpaK11BI GGWCC 5 cut(s) 247, 375, 731, 744, 913
VspI ATTAAT 1 cut(s) 626
XapI RAATTY 6 cut(s) 513, 1430, 1591, 1749, 1804, 1874
XceI RCATGY 2 cut(s) 438, 971
XmaJI CCTAGG 1 cut(s) 893
XmnI GAANNNNTTC 3 cut(s) 1412, 1624, 2141
XspI CTAG 8 cut(s) 75, 141, 335, 894, 1064, 1673, 2028, 2146
ZrmI AGTACT 1 cut(s) 1146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.