Rh4CG246300

acyl-CoA-binding domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0010980
Physical Location & Seq
Forward (+)
49558427 .. 49563592
5166 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG246300.1

Sequence Viewer

Length: 2124 bp
ATGTTCGGTTTTTCTCGCAAACGCATGAAGATTGGACGATTGAAAGTCCAGCTCTCAGATTCTGCTCAGGGAACTAGAAGCCCCATAAGACAGCCGAAACAAAATAATGACTCTGATGCGCCGGCAAGTAGTATTTCTGATGAGCTCGATTGCCAGTGTCCGTCTTCTGCTCCTGAGATTAGTAATTCCACAATGGGGAGCTCTGAGAATTGGATGGTGTTGTCAATTTCTGGAGATAAGCCTACGCCCCGCTTCAATCATGCGGCGGCTGTAATTGGGAATAAGATGATAGTGGTTGGTGGTGAATCTGGGAGTGAATTGCTAGATGATGTACAGGTGCTCAATTTTGACAGTTTTACGTGGAACACAGCTTCGTCAAAGCTTTACTTGTCACCAAGCAGTCTGCCCTTAAAGATCCCTGCATGCAAGGGCCACAGACTGGTTTCCTGGGGGAAAAAGGCACTCCTGGTTGGAGGAAAGACTGAACCTGGGAGTGACAAAATTTCAGTTTGGGCATTTGACACTGAGACAGAATGCTGGTCTCATATGGAAGCAAAGGGTGATATACCGGTTGCTCGTACTGGTCACACTGTAGTCAGGGCAAGTTCTGTATTAATCCTTTTTGGTGGTGAAGATGCTAAAAGACGGAAACTGCATGATCTACATATGTTTGATCTGAAGTCATTAACATGGCTACCTCTTCATTGCACAGGAACGGGACCATCTCCGAGGTCCAACCATGTGGCAGCTCTTTATGATGACAAAACTCTTTTTATATTTGGAGGCACGTCAAAATCCAGGACTTTGAATGATTTGTATTCACTTGACTTTGAAACGATGGTATGGTCAAGAATAAAGAAGCGAGGTTTCCATCCATCACCTAGAGCTGGTTGCTGTGGCGTCTTATGTGGAACCAAATGGTATATCGTTGGTGGTGGAAGCAGAAAAAAACGACATGCGGAGACTCTGATCTTTGATGTTTTGAAACTTGAGTGGTCATTGGCCATTGCATCGCCTCCTTCTACAATCACTACGAATAAGGGTTTCAGCCTAGAGCATGTGCAGCACAAAGAAAAGGACTTTCTTGTTGCTTTTGGGGGATCCAGGAAGGAGCCATCAAATCAGGTTGAAGTACTAACCATGGAAAAGAATGAATTATCCATGAGTCGTCGATCTTTTCCCAGCAAAGGACCTGGAACTTTGATGCCTGAAAAACGCCCATCAACCAATGGCTTGGCTAGTCAACTAACTAACGGTTCTTCCCAGCGCTCAGTCAATTCAGTTGCTAGACAAAATCTGGCATCTGCAATTGAACAACATGGTTCTGGAAGAAAATCTTTGTCAGAGTCCTTGCTTGTCGATCCAAATCCTGTTTCTGGTAATGTCTCACTTAGGAAACAATTTCATAATGACGAAGAATACAACACTTCTGTTAAGGTGACAAAGGGTTCAGAAGATGAAAGTTCTCTCTTGCAGCCCTCAGAAAAGAAGAAAAGTCAATCTGATTCTGCAGTACAAACTAACATACATGGAGGTAGACTTTACTCAGAGGAGATGGTCTCTGCATATGAGTTCGAAAACTCAAATCCCCACAACCATGGACTTGGGAACTTTCCGGTTGATAACAATGATTCAATATATCCAGAAAGTGATAGTAAACCAGGATCACTATCAGCACCTTCAAGTATGTACCAATATTATGAGAAACTAATAAGAAAGAATGGGGTTTTAGAAGGACAGTTGGCTGCTGCATTGGCCAGTCGAGAAGCTGCAGAGAAAAACTTATCCTCTGCTCTCAAGAGCCGACAGGAGATTGAGAGAAAATTAGCAGACTCTGGGAAGGAGATAGAGTTACTGAAGGACAAGCTAGCTGGTGTAGAACTAGCCCAAGAAGAGGCTAACAACATGTCAAGTATTGTTCATTCTGACAATGTGAGGCTTGAGCATGATGTGGCTTTCCTCAAGGCTGTTTTGGATGATACCCAAAAGGAGCTGCATTCAACTAGAGGAGTCCTTGCTGGAGAGCGAGCAAGGGCATTCCAGCTACAGGTTGAAGTTTTTCATCTTAAACAAAGATTACAATCTATGGAGAACCGAGCACCTACACCGAGGAAACCATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_1 Kelch_4 Kelch_5

Protein Analysis

707

Amino Acids

77.71

Weight (kDa)

8.82

Isoelectric Point (pI)

47.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 68 - 125 1.2e-11 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 68 - 127 6e-10 FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 68 - 199 6.4e-12 Attractin/LZTR1 beta-propeller
Kelch_HCF PF13854 72 - 314 3.7e-26 Host cell factor, Kelch-repeats domain
Kelch_1 PF01344 83 - 126 3.8e-08 Kelch motif
Beta-prop_FBX42 PF13415 142 - 265 1.8e-23 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 144 - 350 4.4e-59 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_TYW4 PF13418 145 - 367 1.3e-12 tRNA wybutosine-synthesizing protein 4, beta-propeller
Beta-prop_ATRN-LZTR1 PF24981 156 - 290 1.1e-19 Attractin/LZTR1 beta-propeller
Beta-prop_TYW4 PF13418 190 - 386 5.3e-08 tRNA wybutosine-synthesizing protein 4, beta-propeller
Beta-prop_ATRN-LZTR1 PF24981 206 - 376 6.8e-20 Attractin/LZTR1 beta-propeller
Beta-prop_FBX42 PF13415 247 - 389 1.8e-18 FBX42, beta-propeller domain
ACBP4_C PF24922 640 - 697 1.7e-09 Acyl-CoA-binding domain-containing protein 4-6, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010980)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18590 AT5G18590
fragaria_vesca FvH4_4g17510 FvH4_4g17510 FvH4_4g17510
malus_domestica MD16G1173400.v1.1
prunus_persica Prupe.1G140300_v2.0.a1 Prupe.1G140300_v2.0.a1
pyrus_communis pycom16g14540
rosa_chinensis RchiOBHm_Chr4g0421431
rosa_laevigata RLG00000007685
rosa_multiflora Rmu_sc0001962.1_g000012
rosa_roxburghii Rroxscaffold_5G00363700
rosa_rugosa Rorug04G0171000 Rorug04G0171100
rosa_samantha Rh4AG231800 Rh4BG234700 Rh4CG246300 Rh4DG230300
rosa_wichuraiana Rw4G020230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 439
AccI GTMKAC 1 cut(s) 1537
AciI CCGC 4 cut(s) 250, 263, 266, 959
AclWI GGATC 5 cut(s) 409, 1095, 1108, 1355, 1672
AcoI YGGCCR 2 cut(s) 1002, 1755
AcsI RAATTY 1 cut(s) 501
AcuI CTGAAG 2 cut(s) 698, 1877
AcyI GRCGYC 1 cut(s) 900
AfaI GTAC 5 cut(s) 333, 580, 1134, 1515, 1691
AfeI AGCGCT 1 cut(s) 1268
AfiI CCNNNNNNNGG 7 cut(s) 195, 439, 887, 1187, 1376, 1894, 2047
AflIII ACRYGT 1 cut(s) 1905
AgeI ACCGGT 1 cut(s) 568
AjiI CACGTC 1 cut(s) 789
AjnI CCWGG 7 cut(s) 446, 465, 487, 797, 1103, 1192, 1660
AjuI GAANNNNNNNTTGG 2 cut(s) 1357, 1389
Alw21I GWGCWC 4 cut(s) 147, 203, 342, 2101
Alw26I GTCTC 5 cut(s) 521, 546, 956, 1390, 1564
AlwI GGATC 5 cut(s) 409, 1095, 1108, 1355, 1672
AlwNI CAGNNNCTG 2 cut(s) 62, 1835
Aor51HI AGCGCT 1 cut(s) 1268
AoxI GGCC 3 cut(s) 430, 1002, 1755
ApeKI GCWGC 7 cut(s) 746, 1063, 1474, 1745, 1748, 1769, 1993
ApoI RAATTY 1 cut(s) 501
AseI ATTAAT 1 cut(s) 614
AsiGI ACCGGT 1 cut(s) 568
Asp700I GAANNNNTTC 2 cut(s) 2058, 2117
AspLEI GCGC 2 cut(s) 121, 1269
AspS9I GGNCC 4 cut(s) 430, 719, 732, 1190
AsuHPI GGTGA 6 cut(s) 314, 384, 572, 641, 870, 1450
AsuII TTCGAA 1 cut(s) 1575
AsuNHI GCTAGC 1 cut(s) 1867
AvaII GGWCC 3 cut(s) 719, 732, 1190
BalI TGGCCA 2 cut(s) 1004, 1757
BamHI GGATCC 1 cut(s) 1100
BanII GRGCYC 2 cut(s) 147, 203
BbsI GAAGAC 1 cut(s) 156
Bbv12I GWGCWC 4 cut(s) 147, 203, 342, 2101
BbvI GCAGC 7 cut(s) 758, 1075, 1486, 1732, 1735, 1756, 1980
BccI CCATC 8 cut(s) 208, 730, 832, 879, 883, 1123, 1228, 1549
BciT130I CCWGG 7 cut(s) 448, 467, 489, 799, 1105, 1194, 1662
BcoDI GTCTC 5 cut(s) 521, 546, 956, 1390, 1564
BfmI CTRYAG 4 cut(s) 591, 1509, 1770, 2045
BfoI RGCGCY 1 cut(s) 1270
BisI GCNGC 9 cut(s) 264, 267, 747, 1064, 1475, 1746, 1749, 1770, 1994
BlsI GCNGC 9 cut(s) 265, 268, 748, 1065, 1476, 1747, 1750, 1771, 1995
BmcAI AGTACT 1 cut(s) 1134
Bme1390I CCNGG 7 cut(s) 448, 467, 489, 799, 1105, 1194, 1662
Bme18I GGWCC 3 cut(s) 719, 732, 1190
BmgBI CACGTC 1 cut(s) 789
BmgT120I GGNCC 4 cut(s) 430, 719, 732, 1190
BmiI GGNNCC 4 cut(s) 720, 913, 1102, 1113
BmrFI CCNGG 7 cut(s) 448, 467, 489, 799, 1105, 1194, 1662
BmsI GCATC 5 cut(s) 106, 625, 1019, 1194, 1310
BmtI GCTAGC 1 cut(s) 1871
BpiI GAAGAC 1 cut(s) 156
BplI GAGNNNNNCTC 2 cut(s) 1544, 1576
BpmI CTGGAG 2 cut(s) 252, 2040
Bpu10I CCTNAGC 1 cut(s) 66
Bpu14I TTCGAA 1 cut(s) 1575
BpuEI CTTGAG 4 cut(s) 1010, 1781, 1946, 1961
BsaAI YACGTR 1 cut(s) 360
BsaBI GATNNNNATC 3 cut(s) 1365, 1669, 2080
BsaHI GRCGYC 1 cut(s) 900
BsaI GGTCTC 2 cut(s) 546, 1564
BsaJI CCNNGG 6 cut(s) 447, 488, 728, 1140, 1597, 2108
BsaWI WCCGGW 2 cut(s) 568, 1615
BsaXI ACNNNNNCTCC 2 cut(s) 1835, 1865
Bsc4I CCNNNNNNNGG 7 cut(s) 195, 439, 887, 1187, 1376, 1894, 2047
Bse118I RCCGGY 2 cut(s) 121, 568
Bse1I ACTGG 4 cut(s) 154, 444, 586, 1758
Bse3DI GCAATG 2 cut(s) 703, 1005
Bse8I GATNNNNATC 3 cut(s) 1365, 1669, 2080
BseBI CCWGG 7 cut(s) 448, 467, 489, 799, 1105, 1194, 1662
BseDI CCNNGG 6 cut(s) 447, 488, 728, 1140, 1597, 2108
BseGI GGATG 3 cut(s) 219, 871, 1981
BseJI GATNNNNATC 3 cut(s) 1365, 1669, 2080
BseLI CCNNNNNNNGG 7 cut(s) 195, 439, 887, 1187, 1376, 1894, 2047
BseMI GCAATG 2 cut(s) 703, 1005
BseMII CTCAG 8 cut(s) 69, 80, 165, 195, 516, 1284, 1494, 1560
BseNI ACTGG 4 cut(s) 154, 444, 586, 1758
BseRI GAGGAG 2 cut(s) 1565, 2022
BseXI GCAGC 7 cut(s) 758, 1075, 1486, 1732, 1735, 1756, 1980
BseYI CCCAGC 2 cut(s) 1181, 1263
BsgI GTGCAG 1 cut(s) 1082
BshFI GGCC 3 cut(s) 432, 1004, 1757
BshTI ACCGGT 1 cut(s) 568
BsiHKAI GWGCWC 4 cut(s) 147, 203, 342, 2101
BsiSI CCGG 3 cut(s) 122, 569, 1616
BslFI GGGAC 1 cut(s) 732
BslI CCNNNNNNNGG 7 cut(s) 195, 439, 887, 1187, 1376, 1894, 2047
BsmAI GTCTC 5 cut(s) 521, 546, 956, 1390, 1564
BsmFI GGGAC 1 cut(s) 732
BsmI GAATGC 3 cut(s) 539, 1996, 2036
BsnI GGCC 3 cut(s) 432, 1004, 1757
Bso31I GGTCTC 2 cut(s) 546, 1564
Bsp119I TTCGAA 1 cut(s) 1575
Bsp1286I GDGCHC 4 cut(s) 147, 203, 342, 2101
Bsp1407I TGTACA 1 cut(s) 331
Bsp143I GATC 8 cut(s) 414, 658, 673, 969, 1100, 1172, 1360, 1664
Bsp19I CCATGG 2 cut(s) 1140, 1597
BspACI CCGC 4 cut(s) 250, 263, 266, 959
BspANI GGCC 3 cut(s) 432, 1004, 1757
BspCNI CTCAG 8 cut(s) 68, 79, 166, 196, 517, 1283, 1493, 1559
BspLI GGNNCC 4 cut(s) 720, 913, 1102, 1113
BspMAI CTGCAG 2 cut(s) 1513, 1774
BspOI GCTAGC 1 cut(s) 1871
BspPI GGATC 5 cut(s) 409, 1095, 1108, 1355, 1672
BspT104I TTCGAA 1 cut(s) 1575
BspTNI GGTCTC 2 cut(s) 546, 1564
BsrDI GCAATG 2 cut(s) 703, 1005
BsrFI RCCGGY 2 cut(s) 121, 568
BsrGI TGTACA 1 cut(s) 331
BsrI ACTGG 4 cut(s) 154, 444, 586, 1758
BssAI RCCGGY 2 cut(s) 121, 568
BssECI CCNNGG 6 cut(s) 447, 488, 728, 1140, 1597, 2108
BssMI GATC 8 cut(s) 414, 658, 673, 969, 1100, 1172, 1360, 1664
BssNI GRCGYC 1 cut(s) 900
BssT1I CCWWGG 2 cut(s) 1140, 1597
Bst2UI CCWGG 7 cut(s) 448, 467, 489, 799, 1105, 1194, 1662
Bst4CI ACNGT 4 cut(s) 353, 592, 1256, 1740
Bst6I CTCTTC 2 cut(s) 705, 1887
BstACI GRCGYC 1 cut(s) 900
BstAUI TGTACA 1 cut(s) 331
BstBAI YACGTR 1 cut(s) 360
BstBI TTCGAA 1 cut(s) 1575
BstC8I GCNNGC 4 cut(s) 123, 424, 1869, 2028
BstDEI CTNAG 9 cut(s) 55, 66, 174, 204, 525, 1270, 1391, 1480, 1546
BstDSI CCRYGG 2 cut(s) 1140, 1597
BstF5I GGATG 3 cut(s) 219, 871, 1981
BstH2I RGCGCY 1 cut(s) 1270
BstHHI GCGC 2 cut(s) 121, 1269
BstKTI GATC 8 cut(s) 417, 661, 676, 972, 1103, 1175, 1363, 1667
BstMAI GTCTC 5 cut(s) 521, 546, 956, 1390, 1564
BstMBI GATC 8 cut(s) 414, 658, 673, 969, 1100, 1172, 1360, 1664
BstMWI GCNNNNNNNGC 2 cut(s) 1063, 1754
BstNI CCWGG 7 cut(s) 448, 467, 489, 799, 1105, 1194, 1662
BstNSI RCATGY 4 cut(s) 426, 959, 1061, 1909
BstSCI CCNGG 7 cut(s) 446, 465, 487, 797, 1103, 1192, 1660
BstSFI CTRYAG 4 cut(s) 591, 1509, 1770, 2045
BstV1I GCAGC 7 cut(s) 758, 1075, 1486, 1732, 1735, 1756, 1980
BstV2I GAAGAC 1 cut(s) 156
BstX2I RGATCY 2 cut(s) 414, 1100
BstXI CCANNNNNNTGG 4 cut(s) 742, 1234, 1598, 1604
BstYI RGATCY 2 cut(s) 414, 1100
BsuRI GGCC 3 cut(s) 432, 1004, 1757
BtgI CCRYGG 2 cut(s) 1140, 1597
BtgZI GCGATG 1 cut(s) 996
BtrI CACGTC 1 cut(s) 789
BtsCI GGATG 3 cut(s) 219, 871, 1981
BtsIMutI CAGTG 3 cut(s) 161, 522, 588
Cac8I GCNNGC 4 cut(s) 123, 424, 1869, 2028
CaiI CAGNNNCTG 2 cut(s) 62, 1835
CfoI GCGC 2 cut(s) 121, 1269
Cfr10I RCCGGY 2 cut(s) 121, 568
Cfr13I GGNCC 4 cut(s) 430, 719, 732, 1190
CseI GACGC 1 cut(s) 889
Csp6I GTAC 5 cut(s) 332, 579, 1133, 1514, 1690
CspAI ACCGGT 1 cut(s) 568
CviQI GTAC 5 cut(s) 332, 579, 1133, 1514, 1690
DdeI CTNAG 9 cut(s) 55, 66, 174, 204, 525, 1270, 1391, 1480, 1546
DpnI GATC 8 cut(s) 416, 660, 675, 971, 1102, 1174, 1362, 1666
DpnII GATC 8 cut(s) 414, 658, 673, 969, 1100, 1172, 1360, 1664
EaeI YGGCCR 2 cut(s) 1002, 1755
Eam1104I CTCTTC 2 cut(s) 705, 1887
EarI CTCTTC 2 cut(s) 705, 1887
Ecl136II GAGCTC 2 cut(s) 145, 201
Eco130I CCWWGG 2 cut(s) 1140, 1597
Eco24I GRGCYC 2 cut(s) 147, 203
Eco31I GGTCTC 2 cut(s) 546, 1564
Eco47I GGWCC 3 cut(s) 719, 732, 1190
Eco47III AGCGCT 1 cut(s) 1268
Eco53kI GAGCTC 2 cut(s) 145, 201
Eco57I CTGAAG 2 cut(s) 698, 1877
EcoICRI GAGCTC 2 cut(s) 145, 201
EcoO109I RGGNCCY 1 cut(s) 1190
EcoRII CCWGG 7 cut(s) 446, 465, 487, 797, 1103, 1192, 1660
EcoT14I CCWWGG 2 cut(s) 1140, 1597
EcoT38I GRGCYC 2 cut(s) 147, 203
ErhI CCWWGG 2 cut(s) 1140, 1597
FalI AAGNNNNNCTT 4 cut(s) 371, 403, 1321, 1353
FaqI GGGAC 1 cut(s) 732
FauI CCCGC 1 cut(s) 257
FauNDI CATATG 3 cut(s) 546, 666, 1567
FblI GTMKAC 1 cut(s) 1537
Fnu4HI GCNGC 9 cut(s) 264, 267, 747, 1064, 1475, 1746, 1749, 1770, 1994
FokI GGATG 3 cut(s) 226, 858, 1988
FriOI GRGCYC 2 cut(s) 147, 203
Fsp4HI GCNGC 9 cut(s) 264, 267, 747, 1064, 1475, 1746, 1749, 1770, 1994
GlaI GCGC 2 cut(s) 120, 1268
GluI GCNGC 9 cut(s) 264, 267, 747, 1064, 1475, 1746, 1749, 1770, 1994
GsaI CCCAGC 2 cut(s) 1185, 1267
GsuI CTGGAG 2 cut(s) 252, 2040
HaeII RGCGCY 1 cut(s) 1270
HaeIII GGCC 3 cut(s) 432, 1004, 1757
HapII CCGG 3 cut(s) 122, 569, 1616
HgaI GACGC 1 cut(s) 889
HhaI GCGC 2 cut(s) 121, 1269
Hin1I GRCGYC 1 cut(s) 900
Hin6I GCGC 2 cut(s) 119, 1267
HinP1I GCGC 2 cut(s) 119, 1267
HincII GTYRAC 1 cut(s) 1244
HindII GTYRAC 1 cut(s) 1244
HindIII AAGCTT 1 cut(s) 380
HpaII CCGG 3 cut(s) 122, 569, 1616
HphI GGTGA 6 cut(s) 314, 384, 572, 641, 870, 1450
Hpy166II GTNNAC 3 cut(s) 1244, 1538, 1658
Hpy188III TCNNGA 7 cut(s) 173, 231, 849, 1326, 1643, 1763, 1798
Hpy8I GTNNAC 3 cut(s) 1244, 1538, 1658
Hpy99I CGWCG 1 cut(s) 1173
HpyAV CCTTC 6 cut(s) 1029, 1102, 1689, 1727, 1834, 1852
HpyCH4III ACNGT 4 cut(s) 353, 592, 1256, 1740
HpyCH4IV ACGT 2 cut(s) 359, 788
HpyF10VI GCNNNNNNNGC 2 cut(s) 1063, 1754
HpyF3I CTNAG 9 cut(s) 55, 66, 174, 204, 525, 1270, 1391, 1480, 1546
HpySE526I ACGT 2 cut(s) 359, 788
Hsp92I GRCGYC 1 cut(s) 900
HspAI GCGC 2 cut(s) 119, 1267
KroI GCCGGC 1 cut(s) 121
KroNI GCCGGC 1 cut(s) 123
Kzo9I GATC 8 cut(s) 414, 658, 673, 969, 1100, 1172, 1360, 1664
LmnI GCTCC 4 cut(s) 175, 198, 1111, 1990
Lsp1109I GCAGC 7 cut(s) 758, 1075, 1486, 1732, 1735, 1756, 1980
LweI GCATC 5 cut(s) 106, 625, 1019, 1194, 1310
MaeII ACGT 2 cut(s) 359, 788
MaeIII GTNAC 5 cut(s) 390, 494, 584, 1438, 1851
MalI GATC 8 cut(s) 416, 660, 675, 971, 1102, 1174, 1362, 1666
MboI GATC 8 cut(s) 414, 658, 673, 969, 1100, 1172, 1360, 1664
MfeI CAATTG 1 cut(s) 1308
MflI RGATCY 2 cut(s) 414, 1100
MhlI GDGCHC 4 cut(s) 147, 203, 342, 2101
MlsI TGGCCA 2 cut(s) 1004, 1757
MluNI TGGCCA 2 cut(s) 1004, 1757
MlyI GAGTC 6 cut(s) 104, 958, 1174, 1355, 1826, 2019
MmeI TCCRAC 2 cut(s) 451, 759
Mox20I TGGCCA 2 cut(s) 1004, 1757
MroNI GCCGGC 1 cut(s) 121
MroXI GAANNNNTTC 2 cut(s) 2058, 2117
MscI TGGCCA 2 cut(s) 1004, 1757
MseI TTAA 5 cut(s) 410, 614, 686, 1434, 2067
MslI CAYNNNNRTG 2 cut(s) 688, 1596
Msp20I TGGCCA 2 cut(s) 1004, 1757
MspI CCGG 3 cut(s) 122, 569, 1616
MspR9I CCNGG 7 cut(s) 448, 467, 489, 799, 1105, 1194, 1662
MunI CAATTG 1 cut(s) 1308
Mva1269I GAATGC 3 cut(s) 539, 1996, 2036
MvaI CCWGG 7 cut(s) 448, 467, 489, 799, 1105, 1194, 1662
MwoI GCNNNNNNNGC 2 cut(s) 1063, 1754
NaeI GCCGGC 1 cut(s) 123
NcoI CCATGG 2 cut(s) 1140, 1597
NdeI CATATG 3 cut(s) 546, 666, 1567
NdeII GATC 8 cut(s) 414, 658, 673, 969, 1100, 1172, 1360, 1664
NgoMIV GCCGGC 1 cut(s) 121
NheI GCTAGC 1 cut(s) 1867
NlaIV GGNNCC 4 cut(s) 720, 913, 1102, 1113
NmuCI GTSAC 4 cut(s) 390, 494, 584, 1438
NspI RCATGY 4 cut(s) 426, 959, 1061, 1909
NspV TTCGAA 1 cut(s) 1575
PaeI GCATGC 1 cut(s) 426
PciI ACATGT 1 cut(s) 1905
PctI GAATGC 3 cut(s) 539, 1996, 2036
PdiI GCCGGC 1 cut(s) 123
PdmI GAANNNNTTC 2 cut(s) 2058, 2117
PfeI GAWTC 4 cut(s) 59, 305, 1505, 1631
PflMI CCANNNNNTGG 1 cut(s) 439
PfoI TCCNGGA 2 cut(s) 797, 1103
PinAI ACCGGT 1 cut(s) 568
PkrI GCNGC 9 cut(s) 265, 268, 748, 1065, 1476, 1747, 1750, 1771, 1995
PleI GAGTC 6 cut(s) 104, 958, 1173, 1354, 1826, 2018
PpsI GAGTC 6 cut(s) 104, 958, 1173, 1354, 1826, 2018
Ppu21I YACGTR 1 cut(s) 360
PpuMI RGGWCCY 1 cut(s) 1190
PscI ACATGT 1 cut(s) 1905
PshBI ATTAAT 1 cut(s) 614
Psp124BI GAGCTC 2 cut(s) 147, 203
Psp5II RGGWCCY 1 cut(s) 1190
Psp6I CCWGG 7 cut(s) 446, 465, 487, 797, 1103, 1192, 1660
PspFI CCCAGC 2 cut(s) 1181, 1263
PspGI CCWGG 7 cut(s) 446, 465, 487, 797, 1103, 1192, 1660
PspN4I GGNNCC 4 cut(s) 720, 913, 1102, 1113
PspPI GGNCC 4 cut(s) 430, 719, 732, 1190
PspPPI RGGWCCY 1 cut(s) 1190
PstI CTGCAG 2 cut(s) 1513, 1774
PstNI CAGNNNCTG 2 cut(s) 62, 1835
PsuI RGATCY 2 cut(s) 414, 1100
RsaI GTAC 5 cut(s) 333, 580, 1134, 1515, 1691
RsaNI GTAC 5 cut(s) 332, 579, 1133, 1514, 1690
RseI CAYNNNNRTG 2 cut(s) 688, 1596
SacI GAGCTC 2 cut(s) 147, 203
SaqAI TTAA 5 cut(s) 410, 614, 686, 1434, 2067
SatI GCNGC 9 cut(s) 264, 267, 747, 1064, 1475, 1746, 1749, 1770, 1994
Sau3AI GATC 8 cut(s) 414, 658, 673, 969, 1100, 1172, 1360, 1664
Sau96I GGNCC 4 cut(s) 430, 719, 732, 1190
ScaI AGTACT 1 cut(s) 1134
SchI GAGTC 6 cut(s) 104, 958, 1174, 1355, 1826, 2019
ScrFI CCNGG 7 cut(s) 448, 467, 489, 799, 1105, 1194, 1662
SduI GDGCHC 4 cut(s) 147, 203, 342, 2101
SfaNI GCATC 5 cut(s) 106, 625, 1019, 1194, 1310
SfcI CTRYAG 4 cut(s) 591, 1509, 1770, 2045
SfuI TTCGAA 1 cut(s) 1575
SinI GGWCC 3 cut(s) 719, 732, 1190
SmiMI CAYNNNNRTG 2 cut(s) 688, 1596
SmlI CTYRAG 4 cut(s) 989, 1796, 1940, 1961
SmoI CTYRAG 4 cut(s) 989, 1796, 1940, 1961
SphI GCATGC 1 cut(s) 426
SsiI CCGC 4 cut(s) 250, 263, 266, 959
SspI AATATT 1 cut(s) 1697
SstI GAGCTC 2 cut(s) 147, 203
StyD4I CCNGG 7 cut(s) 446, 465, 487, 797, 1103, 1192, 1660
StyI CCWWGG 2 cut(s) 1140, 1597
TaaI ACNGT 4 cut(s) 353, 592, 1256, 1740
TaiI ACGT 2 cut(s) 362, 791
TaqI TCGA 5 cut(s) 147, 1171, 1359, 1575, 1762
TatI WGTACW 3 cut(s) 331, 1132, 1513
TauI GCSGC 2 cut(s) 266, 269
TfiI GAWTC 4 cut(s) 59, 305, 1505, 1631
Tru1I TTAA 5 cut(s) 410, 614, 686, 1434, 2067
Tru9I TTAA 5 cut(s) 410, 614, 686, 1434, 2067
TscAI CASTG 3 cut(s) 161, 529, 595
TseFI GTSAC 4 cut(s) 390, 494, 584, 1438
TseI GCWGC 7 cut(s) 746, 1063, 1474, 1745, 1748, 1769, 1993
Tsp45I GTSAC 4 cut(s) 390, 494, 584, 1438
TspDTI ATGAA 7 cut(s) 41, 692, 1167, 1394, 1473, 1910, 2051
TspGWI ACGGA 2 cut(s) 150, 661
TspRI CASTG 3 cut(s) 161, 529, 595
Van91I CCANNNNNTGG 1 cut(s) 439
VpaK11BI GGWCC 3 cut(s) 719, 732, 1190
VspI ATTAAT 1 cut(s) 614
XapI RAATTY 1 cut(s) 501
XceI RCATGY 4 cut(s) 426, 959, 1061, 1909
XmiI GTMKAC 1 cut(s) 1537
XmnI GAANNNNTTC 2 cut(s) 2058, 2117
ZrmI AGTACT 1 cut(s) 1134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.