Rw4G020230

acyl-CoA-binding domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0010980
Physical Location & Seq
Reverse (-)
43975880 .. 43981107
5228 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G020230.1

Sequence Viewer

Length: 2136 bp
ATGTTCGGTTTTTCTCGCAAACGCATGAAGATTGGACGATTGAAAGTCCAGCTCTCAGATTCTGCTCAGGGAACTAGAAGCCCCATAAGACAGCCGAAACAAAATAACAGCTCAAATAATGACTCTGATGCGCCGGCAAGTAGTATTTCTGATGAGCTCGATTGCCAGTGTCCGTCTTCTGCTCCTGAGATTAGTAATTCCACAATGGGGAGCTCTGAGAATTGGATGGTGTTGTCAATTTCTGGAGATAAGCCTACGCCCCGCTTCAATCATGCGGCGGCTGTAATTGGGAATAAGATGATAGTGGTTGGTGGTGAATCTGGGAGTGAACTGCTAGATGATGTACAGGTGCTCAATTTTGACAGTTTTACGTGGAACACAGCTTCGTCAAAGCTTTACTTGTCACCAAGCAGTCTGCCCTTAAAGATCCCTGCATGCAGGGGCCACAGACTGGTTTCCTGGGGGAAAAAGGCACTCCTGGTTGGAGGAAAGACTGAACCTGGGAGTGACAAAATTGCAGTTTGGGCATTTGACACTGAGACAGAATGCTGGTCTCATATGGAAGCAAAGGGTGATATACCGGTTGCTCGTTCTGGTCACACTGTAGTCAGGGCAAGTTCTGTATTAATCCTTTTTGGTGGTGAAGATGCTAAAAGACGGAAACTGCATGATCTACATATGTTTGATCTGAAGTCATTAACATGGCTACCTCTTCATTGCACAGGAACGGGACCATCTCCGAGGTCCAACCATGTGGCAGCTCTTTATGATGACAAAACTCTTTTTATATTTGGTGGCACGTCAAAATCCAGGACTTTGAATGATTTGTATTCACTTGACTTTGAAACGATGGTATGGTCAAGAATAAAGAAGCGAGGTTTCCATCCATCACCTAGAGCTGGTTGCTGTGGGGTCTTATGTGGAACCAAATGGTATATCGTTGGTGGTGGAAGCAGAAAAAAACGACATGCGGAGACTCTGATCTTTGATGTTTTGAAACTTGAGTGGTCATTGGCCATTGCATCGCCTCCTTCTACAATCACTACGAATAAGGGTTTCAGCCTAGAGCATGTGCAGCACAAAGAAAAGGATTTTCTTGTTGCTTTTGGGGGATCCAGGAAGGAGCCATCAAATCAGGTTGAAGTACTAACCATGGAAAAGAATGAATTATCCATGAGTCGTCGATCTTTTCCCAGCAAAGGACCTGGAACTTTGATGCCTGAAAAACGCCCATCAACCAATGGCTTGGCTAGTCAACTAACTAACGGTTCTTCCCAGCGCTCAGTCAATTCAGTTGCTAGACAAAATCTGGCATCTGCAATTGAACAACATGGTTCTGGAAGAAAATCTTTGTCAGAGTCCTTGCTTGTCGATCCAAATCCTGTTTCTGGTAATGTCTCACTTAGGAAACAATTTCATAATGACGAAGAATACAACATTTCTGTTAAGGTGACAAAGGGTTCAGAAGATGAAAGTTCTCTCTTGCAGCCCTCAGAAAAGAAGAAAAGTCAATCTGATTCTGCAGTACAAACTAACATACATGGAGGTAGACTTTACTCAGAGGAGATGGTCTCTGCATATGAGTTCGAAAACTCAAATCCCCACAACCATGGACTTGGGAACTTTCCGGTTGATAACAATGATTCAATATATCCAGAAAGTGATAGTAAACCAGGATCACTATCAGCGCCTTCAAGTATGTACCAATATTATGAGAAACTAATAAGAAAGAATGGGGTTTTAGAAGGACAGTTGGCTGCTGCATTGGCCAGTCGAGAAGCTGCAGAGAAAAACTTATCCTCTGCTCTCAAGAGCCGACAGGAGATTGAGAGAAAATTAGCAGACTCTGGGAAGGAGATAGAGTTACTGAAGGACAAGCTAGCTGGTGTAGAACTAGCCCAAGAAGAGGCTAACAACATGTCAAGTATTGTTCATTCTGACAATGTGAGGCTTGAGCATGATGTGGCTTTCCTCAAGGCTGTTTTGGATGATACCCAAAAGGAGCTGCATTCAACTAGAGGAGTCCTTGCTGGAGAGCGAGCAAGGGCATTCCAGCTACAGGTTGAAGTTTTTCATCTTAAACAAAGATTACAATCTATGGAGAACCGAGCACCTACACCGAGGAAACCATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_1 Kelch_4 Kelch_5

Protein Analysis

711

Amino Acids

78.12

Weight (kDa)

8.82

Isoelectric Point (pI)

49.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 72 - 129 1.2e-11 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 72 - 131 6e-10 FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 72 - 203 2.4e-11 Attractin/LZTR1 beta-propeller
Kelch_HCF PF13854 76 - 318 1.5e-25 Host cell factor, Kelch-repeats domain
Kelch_1 PF01344 87 - 130 3.8e-08 Kelch motif
Beta-prop_FBX42 PF13415 146 - 269 2e-23 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 148 - 354 6e-59 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_TYW4 PF13418 149 - 371 8e-13 tRNA wybutosine-synthesizing protein 4, beta-propeller
Beta-prop_ATRN-LZTR1 PF24981 210 - 380 7.8e-20 Attractin/LZTR1 beta-propeller
Beta-prop_FBX42 PF13415 251 - 393 1.9e-18 FBX42, beta-propeller domain
ACBP4_C PF24922 644 - 701 1.7e-09 Acyl-CoA-binding domain-containing protein 4-6, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010980)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18590 AT5G18590
fragaria_vesca FvH4_4g17510 FvH4_4g17510 FvH4_4g17510
malus_domestica MD16G1173400.v1.1
prunus_persica Prupe.1G140300_v2.0.a1 Prupe.1G140300_v2.0.a1
pyrus_communis pycom16g14540
rosa_chinensis RchiOBHm_Chr4g0421431
rosa_laevigata RLG00000007685
rosa_multiflora Rmu_sc0001962.1_g000012
rosa_roxburghii Rroxscaffold_5G00363700
rosa_rugosa Rorug04G0171000 Rorug04G0171100
rosa_samantha Rh4AG231800 Rh4BG234700 Rh4CG246300 Rh4DG230300
rosa_wichuraiana Rw4G020230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 451
AccI GTMKAC 1 cut(s) 1549
AciI CCGC 4 cut(s) 262, 275, 278, 971
AclWI GGATC 5 cut(s) 421, 1107, 1120, 1367, 1684
AcoI YGGCCR 2 cut(s) 1014, 1767
AcuI CTGAAG 2 cut(s) 710, 1889
AfaI GTAC 4 cut(s) 345, 1146, 1527, 1703
AfeI AGCGCT 1 cut(s) 1280
AfiI CCNNNNNNNGG 7 cut(s) 207, 451, 899, 1199, 1388, 1906, 2059
AflIII ACRYGT 1 cut(s) 1917
AgeI ACCGGT 1 cut(s) 580
AjiI CACGTC 1 cut(s) 801
AjnI CCWGG 7 cut(s) 458, 477, 499, 809, 1115, 1204, 1672
AjuI GAANNNNNNNTTGG 2 cut(s) 1369, 1401
Alw21I GWGCWC 4 cut(s) 159, 215, 354, 2113
Alw26I GTCTC 5 cut(s) 533, 558, 968, 1402, 1576
AlwI GGATC 5 cut(s) 421, 1107, 1120, 1367, 1684
AlwNI CAGNNNCTG 2 cut(s) 62, 1847
Aor51HI AGCGCT 1 cut(s) 1280
AoxI GGCC 3 cut(s) 442, 1014, 1767
ApeKI GCWGC 7 cut(s) 758, 1075, 1486, 1757, 1760, 1781, 2005
AseI ATTAAT 1 cut(s) 626
AsiGI ACCGGT 1 cut(s) 580
Asp700I GAANNNNTTC 2 cut(s) 2070, 2129
AspLEI GCGC 3 cut(s) 133, 1281, 1690
AspS9I GGNCC 4 cut(s) 442, 731, 744, 1202
AsuHPI GGTGA 6 cut(s) 326, 396, 584, 653, 882, 1462
AsuII TTCGAA 1 cut(s) 1587
AsuNHI GCTAGC 1 cut(s) 1879
AvaII GGWCC 3 cut(s) 731, 744, 1202
BalI TGGCCA 2 cut(s) 1016, 1769
BamHI GGATCC 1 cut(s) 1112
BanII GRGCYC 2 cut(s) 159, 215
BbsI GAAGAC 1 cut(s) 168
Bbv12I GWGCWC 4 cut(s) 159, 215, 354, 2113
BbvI GCAGC 7 cut(s) 770, 1087, 1498, 1744, 1747, 1768, 1992
BccI CCATC 8 cut(s) 220, 742, 844, 891, 895, 1135, 1240, 1561
BciT130I CCWGG 7 cut(s) 460, 479, 501, 811, 1117, 1206, 1674
BcoDI GTCTC 5 cut(s) 533, 558, 968, 1402, 1576
BfmI CTRYAG 4 cut(s) 603, 1521, 1782, 2057
BfoI RGCGCY 2 cut(s) 1282, 1691
BisI GCNGC 9 cut(s) 276, 279, 759, 1076, 1487, 1758, 1761, 1782, 2006
BlsI GCNGC 9 cut(s) 277, 280, 760, 1077, 1488, 1759, 1762, 1783, 2007
BmcAI AGTACT 1 cut(s) 1146
Bme1390I CCNGG 7 cut(s) 460, 479, 501, 811, 1117, 1206, 1674
Bme18I GGWCC 3 cut(s) 731, 744, 1202
BmgBI CACGTC 1 cut(s) 801
BmgT120I GGNCC 4 cut(s) 442, 731, 744, 1202
BmiI GGNNCC 5 cut(s) 443, 732, 925, 1114, 1125
BmrFI CCNGG 7 cut(s) 460, 479, 501, 811, 1117, 1206, 1674
BmsI GCATC 5 cut(s) 118, 637, 1031, 1206, 1322
BmtI GCTAGC 1 cut(s) 1883
BpiI GAAGAC 1 cut(s) 168
BplI GAGNNNNNCTC 2 cut(s) 1556, 1588
BpmI CTGGAG 2 cut(s) 264, 2052
Bpu10I CCTNAGC 1 cut(s) 66
Bpu14I TTCGAA 1 cut(s) 1587
BpuEI CTTGAG 4 cut(s) 1022, 1793, 1958, 1973
BsaAI YACGTR 1 cut(s) 372
BsaBI GATNNNNATC 3 cut(s) 1377, 1681, 2092
BsaI GGTCTC 2 cut(s) 558, 1576
BsaJI CCNNGG 6 cut(s) 459, 500, 740, 1152, 1609, 2120
BsaWI WCCGGW 2 cut(s) 580, 1627
BsaXI ACNNNNNCTCC 2 cut(s) 1847, 1877
Bsc4I CCNNNNNNNGG 7 cut(s) 207, 451, 899, 1199, 1388, 1906, 2059
Bse118I RCCGGY 2 cut(s) 133, 580
Bse1I ACTGG 3 cut(s) 166, 456, 1770
Bse3DI GCAATG 2 cut(s) 715, 1017
Bse8I GATNNNNATC 3 cut(s) 1377, 1681, 2092
BseBI CCWGG 7 cut(s) 460, 479, 501, 811, 1117, 1206, 1674
BseDI CCNNGG 6 cut(s) 459, 500, 740, 1152, 1609, 2120
BseGI GGATG 3 cut(s) 231, 883, 1993
BseJI GATNNNNATC 3 cut(s) 1377, 1681, 2092
BseLI CCNNNNNNNGG 7 cut(s) 207, 451, 899, 1199, 1388, 1906, 2059
BseMI GCAATG 2 cut(s) 715, 1017
BseMII CTCAG 8 cut(s) 69, 80, 177, 207, 528, 1296, 1506, 1572
BseNI ACTGG 3 cut(s) 166, 456, 1770
BseRI GAGGAG 2 cut(s) 1577, 2034
BseXI GCAGC 7 cut(s) 770, 1087, 1498, 1744, 1747, 1768, 1992
BseYI CCCAGC 2 cut(s) 1193, 1275
BsgI GTGCAG 1 cut(s) 1094
BshFI GGCC 3 cut(s) 444, 1016, 1769
BshTI ACCGGT 1 cut(s) 580
BsiHKAI GWGCWC 4 cut(s) 159, 215, 354, 2113
BsiSI CCGG 3 cut(s) 134, 581, 1628
BslFI GGGAC 1 cut(s) 744
BslI CCNNNNNNNGG 7 cut(s) 207, 451, 899, 1199, 1388, 1906, 2059
BsmAI GTCTC 5 cut(s) 533, 558, 968, 1402, 1576
BsmFI GGGAC 1 cut(s) 744
BsmI GAATGC 3 cut(s) 551, 2008, 2048
BsnI GGCC 3 cut(s) 444, 1016, 1769
Bso31I GGTCTC 2 cut(s) 558, 1576
Bsp119I TTCGAA 1 cut(s) 1587
Bsp1286I GDGCHC 4 cut(s) 159, 215, 354, 2113
Bsp1407I TGTACA 1 cut(s) 343
Bsp143I GATC 8 cut(s) 426, 670, 685, 981, 1112, 1184, 1372, 1676
Bsp19I CCATGG 2 cut(s) 1152, 1609
BspACI CCGC 4 cut(s) 262, 275, 278, 971
BspANI GGCC 3 cut(s) 444, 1016, 1769
BspCNI CTCAG 8 cut(s) 68, 79, 178, 208, 529, 1295, 1505, 1571
BspLI GGNNCC 5 cut(s) 443, 732, 925, 1114, 1125
BspMAI CTGCAG 2 cut(s) 1525, 1786
BspOI GCTAGC 1 cut(s) 1883
BspPI GGATC 5 cut(s) 421, 1107, 1120, 1367, 1684
BspT104I TTCGAA 1 cut(s) 1587
BspTNI GGTCTC 2 cut(s) 558, 1576
BsrDI GCAATG 2 cut(s) 715, 1017
BsrFI RCCGGY 2 cut(s) 133, 580
BsrGI TGTACA 1 cut(s) 343
BsrI ACTGG 3 cut(s) 166, 456, 1770
BssAI RCCGGY 2 cut(s) 133, 580
BssECI CCNNGG 6 cut(s) 459, 500, 740, 1152, 1609, 2120
BssMI GATC 8 cut(s) 426, 670, 685, 981, 1112, 1184, 1372, 1676
BssT1I CCWWGG 2 cut(s) 1152, 1609
Bst2UI CCWGG 7 cut(s) 460, 479, 501, 811, 1117, 1206, 1674
Bst4CI ACNGT 4 cut(s) 365, 604, 1268, 1752
Bst6I CTCTTC 2 cut(s) 717, 1899
BstAUI TGTACA 1 cut(s) 343
BstBAI YACGTR 1 cut(s) 372
BstBI TTCGAA 1 cut(s) 1587
BstC8I GCNNGC 4 cut(s) 135, 436, 1881, 2040
BstDEI CTNAG 9 cut(s) 55, 66, 186, 216, 537, 1282, 1403, 1492, 1558
BstDSI CCRYGG 2 cut(s) 1152, 1609
BstF5I GGATG 3 cut(s) 231, 883, 1993
BstH2I RGCGCY 2 cut(s) 1282, 1691
BstHHI GCGC 3 cut(s) 133, 1281, 1690
BstKTI GATC 8 cut(s) 429, 673, 688, 984, 1115, 1187, 1375, 1679
BstMAI GTCTC 5 cut(s) 533, 558, 968, 1402, 1576
BstMBI GATC 8 cut(s) 426, 670, 685, 981, 1112, 1184, 1372, 1676
BstMWI GCNNNNNNNGC 3 cut(s) 524, 1075, 1766
BstNI CCWGG 7 cut(s) 460, 479, 501, 811, 1117, 1206, 1674
BstNSI RCATGY 4 cut(s) 438, 971, 1073, 1921
BstSCI CCNGG 7 cut(s) 458, 477, 499, 809, 1115, 1204, 1672
BstSFI CTRYAG 4 cut(s) 603, 1521, 1782, 2057
BstV1I GCAGC 7 cut(s) 770, 1087, 1498, 1744, 1747, 1768, 1992
BstV2I GAAGAC 1 cut(s) 168
BstX2I RGATCY 2 cut(s) 426, 1112
BstXI CCANNNNNNTGG 4 cut(s) 754, 1246, 1610, 1616
BstYI RGATCY 2 cut(s) 426, 1112
BsuRI GGCC 3 cut(s) 444, 1016, 1769
BtgI CCRYGG 2 cut(s) 1152, 1609
BtgZI GCGATG 1 cut(s) 1008
BtrI CACGTC 1 cut(s) 801
BtsCI GGATG 3 cut(s) 231, 883, 1993
BtsIMutI CAGTG 3 cut(s) 173, 534, 600
Cac8I GCNNGC 4 cut(s) 135, 436, 1881, 2040
CaiI CAGNNNCTG 2 cut(s) 62, 1847
CfoI GCGC 3 cut(s) 133, 1281, 1690
Cfr10I RCCGGY 2 cut(s) 133, 580
Cfr13I GGNCC 4 cut(s) 442, 731, 744, 1202
Csp6I GTAC 4 cut(s) 344, 1145, 1526, 1702
CspAI ACCGGT 1 cut(s) 580
CviQI GTAC 4 cut(s) 344, 1145, 1526, 1702
DdeI CTNAG 9 cut(s) 55, 66, 186, 216, 537, 1282, 1403, 1492, 1558
DpnI GATC 8 cut(s) 428, 672, 687, 983, 1114, 1186, 1374, 1678
DpnII GATC 8 cut(s) 426, 670, 685, 981, 1112, 1184, 1372, 1676
EaeI YGGCCR 2 cut(s) 1014, 1767
Eam1104I CTCTTC 2 cut(s) 717, 1899
EarI CTCTTC 2 cut(s) 717, 1899
Ecl136II GAGCTC 2 cut(s) 157, 213
Eco130I CCWWGG 2 cut(s) 1152, 1609
Eco24I GRGCYC 2 cut(s) 159, 215
Eco31I GGTCTC 2 cut(s) 558, 1576
Eco47I GGWCC 3 cut(s) 731, 744, 1202
Eco47III AGCGCT 1 cut(s) 1280
Eco53kI GAGCTC 2 cut(s) 157, 213
Eco57I CTGAAG 2 cut(s) 710, 1889
EcoICRI GAGCTC 2 cut(s) 157, 213
EcoO109I RGGNCCY 1 cut(s) 1202
EcoRII CCWGG 7 cut(s) 458, 477, 499, 809, 1115, 1204, 1672
EcoT14I CCWWGG 2 cut(s) 1152, 1609
EcoT38I GRGCYC 2 cut(s) 159, 215
ErhI CCWWGG 2 cut(s) 1152, 1609
FalI AAGNNNNNCTT 4 cut(s) 383, 415, 1333, 1365
FaqI GGGAC 1 cut(s) 744
FauI CCCGC 1 cut(s) 269
FauNDI CATATG 3 cut(s) 558, 678, 1579
FblI GTMKAC 1 cut(s) 1549
Fnu4HI GCNGC 9 cut(s) 276, 279, 759, 1076, 1487, 1758, 1761, 1782, 2006
FokI GGATG 3 cut(s) 238, 870, 2000
FriOI GRGCYC 2 cut(s) 159, 215
Fsp4HI GCNGC 9 cut(s) 276, 279, 759, 1076, 1487, 1758, 1761, 1782, 2006
GlaI GCGC 3 cut(s) 132, 1280, 1689
GluI GCNGC 9 cut(s) 276, 279, 759, 1076, 1487, 1758, 1761, 1782, 2006
GsaI CCCAGC 2 cut(s) 1197, 1279
GsuI CTGGAG 2 cut(s) 264, 2052
HaeII RGCGCY 2 cut(s) 1282, 1691
HaeIII GGCC 3 cut(s) 444, 1016, 1769
HapII CCGG 3 cut(s) 134, 581, 1628
HhaI GCGC 3 cut(s) 133, 1281, 1690
Hin6I GCGC 3 cut(s) 131, 1279, 1688
HinP1I GCGC 3 cut(s) 131, 1279, 1688
HincII GTYRAC 1 cut(s) 1256
HindII GTYRAC 1 cut(s) 1256
HindIII AAGCTT 1 cut(s) 392
HpaII CCGG 3 cut(s) 134, 581, 1628
HphI GGTGA 6 cut(s) 326, 396, 584, 653, 882, 1462
Hpy166II GTNNAC 4 cut(s) 329, 1256, 1550, 1670
Hpy188III TCNNGA 7 cut(s) 185, 243, 861, 1338, 1655, 1775, 1810
Hpy8I GTNNAC 4 cut(s) 329, 1256, 1550, 1670
Hpy99I CGWCG 1 cut(s) 1185
HpyAV CCTTC 6 cut(s) 1041, 1114, 1701, 1739, 1846, 1864
HpyCH4III ACNGT 4 cut(s) 365, 604, 1268, 1752
HpyCH4IV ACGT 2 cut(s) 371, 800
HpyF10VI GCNNNNNNNGC 3 cut(s) 524, 1075, 1766
HpyF3I CTNAG 9 cut(s) 55, 66, 186, 216, 537, 1282, 1403, 1492, 1558
HpySE526I ACGT 2 cut(s) 371, 800
HspAI GCGC 3 cut(s) 131, 1279, 1688
KroI GCCGGC 1 cut(s) 133
KroNI GCCGGC 1 cut(s) 135
Kzo9I GATC 8 cut(s) 426, 670, 685, 981, 1112, 1184, 1372, 1676
LmnI GCTCC 4 cut(s) 187, 210, 1123, 2002
Lsp1109I GCAGC 7 cut(s) 770, 1087, 1498, 1744, 1747, 1768, 1992
LweI GCATC 5 cut(s) 118, 637, 1031, 1206, 1322
MaeII ACGT 2 cut(s) 371, 800
MaeIII GTNAC 5 cut(s) 402, 506, 596, 1450, 1863
MalI GATC 8 cut(s) 428, 672, 687, 983, 1114, 1186, 1374, 1678
MboI GATC 8 cut(s) 426, 670, 685, 981, 1112, 1184, 1372, 1676
MfeI CAATTG 1 cut(s) 1320
MflI RGATCY 2 cut(s) 426, 1112
MhlI GDGCHC 4 cut(s) 159, 215, 354, 2113
MlsI TGGCCA 2 cut(s) 1016, 1769
MluNI TGGCCA 2 cut(s) 1016, 1769
MlyI GAGTC 6 cut(s) 116, 970, 1186, 1367, 1838, 2031
MmeI TCCRAC 2 cut(s) 463, 771
Mox20I TGGCCA 2 cut(s) 1016, 1769
MroNI GCCGGC 1 cut(s) 133
MroXI GAANNNNTTC 2 cut(s) 2070, 2129
MscI TGGCCA 2 cut(s) 1016, 1769
MseI TTAA 5 cut(s) 422, 626, 698, 1446, 2079
MslI CAYNNNNRTG 2 cut(s) 700, 1608
Msp20I TGGCCA 2 cut(s) 1016, 1769
MspI CCGG 3 cut(s) 134, 581, 1628
MspR9I CCNGG 7 cut(s) 460, 479, 501, 811, 1117, 1206, 1674
MunI CAATTG 1 cut(s) 1320
Mva1269I GAATGC 3 cut(s) 551, 2008, 2048
MvaI CCWGG 7 cut(s) 460, 479, 501, 811, 1117, 1206, 1674
MwoI GCNNNNNNNGC 3 cut(s) 524, 1075, 1766
NaeI GCCGGC 1 cut(s) 135
NcoI CCATGG 2 cut(s) 1152, 1609
NdeI CATATG 3 cut(s) 558, 678, 1579
NdeII GATC 8 cut(s) 426, 670, 685, 981, 1112, 1184, 1372, 1676
NgoMIV GCCGGC 1 cut(s) 133
NheI GCTAGC 1 cut(s) 1879
NlaIV GGNNCC 5 cut(s) 443, 732, 925, 1114, 1125
NmuCI GTSAC 4 cut(s) 402, 506, 596, 1450
NspI RCATGY 4 cut(s) 438, 971, 1073, 1921
NspV TTCGAA 1 cut(s) 1587
PaeI GCATGC 1 cut(s) 438
PciI ACATGT 1 cut(s) 1917
PctI GAATGC 3 cut(s) 551, 2008, 2048
PdiI GCCGGC 1 cut(s) 135
PdmI GAANNNNTTC 2 cut(s) 2070, 2129
PfeI GAWTC 4 cut(s) 59, 317, 1517, 1643
PflMI CCANNNNNTGG 1 cut(s) 451
PfoI TCCNGGA 2 cut(s) 809, 1115
PinAI ACCGGT 1 cut(s) 580
PkrI GCNGC 9 cut(s) 277, 280, 760, 1077, 1488, 1759, 1762, 1783, 2007
PleI GAGTC 6 cut(s) 116, 970, 1185, 1366, 1838, 2030
PpsI GAGTC 6 cut(s) 116, 970, 1185, 1366, 1838, 2030
Ppu21I YACGTR 1 cut(s) 372
PpuMI RGGWCCY 1 cut(s) 1202
PscI ACATGT 1 cut(s) 1917
PshBI ATTAAT 1 cut(s) 626
Psp124BI GAGCTC 2 cut(s) 159, 215
Psp5II RGGWCCY 1 cut(s) 1202
Psp6I CCWGG 7 cut(s) 458, 477, 499, 809, 1115, 1204, 1672
PspFI CCCAGC 2 cut(s) 1193, 1275
PspGI CCWGG 7 cut(s) 458, 477, 499, 809, 1115, 1204, 1672
PspN4I GGNNCC 5 cut(s) 443, 732, 925, 1114, 1125
PspPI GGNCC 4 cut(s) 442, 731, 744, 1202
PspPPI RGGWCCY 1 cut(s) 1202
PstI CTGCAG 2 cut(s) 1525, 1786
PstNI CAGNNNCTG 2 cut(s) 62, 1847
PsuI RGATCY 2 cut(s) 426, 1112
RsaI GTAC 4 cut(s) 345, 1146, 1527, 1703
RsaNI GTAC 4 cut(s) 344, 1145, 1526, 1702
RseI CAYNNNNRTG 2 cut(s) 700, 1608
SacI GAGCTC 2 cut(s) 159, 215
SaqAI TTAA 5 cut(s) 422, 626, 698, 1446, 2079
SatI GCNGC 9 cut(s) 276, 279, 759, 1076, 1487, 1758, 1761, 1782, 2006
Sau3AI GATC 8 cut(s) 426, 670, 685, 981, 1112, 1184, 1372, 1676
Sau96I GGNCC 4 cut(s) 442, 731, 744, 1202
ScaI AGTACT 1 cut(s) 1146
SchI GAGTC 6 cut(s) 116, 970, 1186, 1367, 1838, 2031
ScrFI CCNGG 7 cut(s) 460, 479, 501, 811, 1117, 1206, 1674
SduI GDGCHC 4 cut(s) 159, 215, 354, 2113
SfaNI GCATC 5 cut(s) 118, 637, 1031, 1206, 1322
SfcI CTRYAG 4 cut(s) 603, 1521, 1782, 2057
SfuI TTCGAA 1 cut(s) 1587
SinI GGWCC 3 cut(s) 731, 744, 1202
SmiMI CAYNNNNRTG 2 cut(s) 700, 1608
SmlI CTYRAG 4 cut(s) 1001, 1808, 1952, 1973
SmoI CTYRAG 4 cut(s) 1001, 1808, 1952, 1973
SphI GCATGC 1 cut(s) 438
SsiI CCGC 4 cut(s) 262, 275, 278, 971
SspI AATATT 1 cut(s) 1709
SstI GAGCTC 2 cut(s) 159, 215
StyD4I CCNGG 7 cut(s) 458, 477, 499, 809, 1115, 1204, 1672
StyI CCWWGG 2 cut(s) 1152, 1609
TaaI ACNGT 4 cut(s) 365, 604, 1268, 1752
TaiI ACGT 2 cut(s) 374, 803
TaqI TCGA 5 cut(s) 159, 1183, 1371, 1587, 1774
TatI WGTACW 3 cut(s) 343, 1144, 1525
TauI GCSGC 2 cut(s) 278, 281
TfiI GAWTC 4 cut(s) 59, 317, 1517, 1643
Tru1I TTAA 5 cut(s) 422, 626, 698, 1446, 2079
Tru9I TTAA 5 cut(s) 422, 626, 698, 1446, 2079
TscAI CASTG 3 cut(s) 173, 541, 607
TseFI GTSAC 4 cut(s) 402, 506, 596, 1450
TseI GCWGC 7 cut(s) 758, 1075, 1486, 1757, 1760, 1781, 2005
Tsp45I GTSAC 4 cut(s) 402, 506, 596, 1450
TspDTI ATGAA 7 cut(s) 41, 704, 1179, 1406, 1485, 1922, 2063
TspGWI ACGGA 2 cut(s) 162, 673
TspRI CASTG 3 cut(s) 173, 541, 607
Van91I CCANNNNNTGG 1 cut(s) 451
VpaK11BI GGWCC 3 cut(s) 731, 744, 1202
VspI ATTAAT 1 cut(s) 626
XceI RCATGY 4 cut(s) 438, 971, 1073, 1921
XmiI GTMKAC 1 cut(s) 1549
XmnI GAANNNNTTC 2 cut(s) 2070, 2129
ZrmI AGTACT 1 cut(s) 1146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.