Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0009955

Physical Location & Seq

Genomic Coordinates Forward (+)

15116826 .. 15122544

Gene Structure 5719 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1177600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2469 bp

ATGCAAAGCCCGCCCCCTCTCTCTCCAACGCAATCGCAACGGCAGCGTTTAGACCAACCACCTAGGGTTTCACAGATGGAAATTCAACCGCCGCTGCCCTCCGACGACACCTCCACCGTCTGGGAATGGGGAGATCTCTTCGATTTCACCGTCGACGATGACTTGTCGATCTCGTGGGGCTCCGCCGAGTATCCGGACCCCGAAGTCGGCCCGGCCCCGGAGCACCTGCTGGAGGATCCGAATTCCAATTCGAGTCGGATAAGGAAGCGGGACCCGAGGCTGGCTTGCCCCAACTTCTTGGCGGGTCATATCCCCTGTGCGTGCCCCGAGATCGACGCGAAGATGATGGAGCTGGAGGAGGAGGAAGCCGGGCACGGGAAGAAGCGGGTCAAGACGGGTCGGGCCCCGAACGGAACGGCCCGGTGTCAGGTCCCGTCTTGCGGCGTCGATATCAAGGAGCTGAAGGGGTATCATCGGAGGCACAGAGTGTGCCTTGCCTGTGCAAATGCCAGCACTGTCGATATCGACGGTGAGAGCAAGCGCTATTGTCAGCAGTGTGGCAAGTTCCATGTCTTATCAGACTTTGATGAAGGTAAGCGGAGCTGTCGAAGAAAATTAGAGCGTCATAATAATAGACGGCGAAGGAAACCTAGTGCTTCTAAAGGAGCGAGTGGAAAGGAATCACAAAAGGAAAATCAAACTGAAGAGACCAATTTTGATGGTGGAGCAACAGAAGATTGTTTACAGTTAAGCAGTCAGTTAAATGAGAAGGAAGAATTGCTGGAGTCTCAAGGTGGGCAAACTCCTATCCTCTGTTCAGTGCCTGAATCCCAGAATATCCGTAGTGACAGCGGTGTATCATTTATGGCTTCTGGTGAAACCCAAATGGATGCAAGAAAACATGATTCTAACAACTCCCTTTCTCCGTCAAATTGTGACAACAAAAGCTACTCTTCTATGTGTCCAACAGGTCGTATATCATTCAAGCTCTATGATTGGAATCCAGCAGAATTTCCTAGAAGACTTCGGCACCAAATATTCGAATGGTTAGCCAGTATGCCGGTTGAGTTGGAGGGATATATCCGCCCCGGATGTACTATCTTGACTATTTTTATTGCAATGCCTAATTTTATGTGGATGAAGTTGCTTGAAGATCCTGTATCATATCTACATGAATTTGTTATTGTACCTGGGAAAATGCTATCCGGGAGAAGCAATATTCTGATTTATCTAAACGACATGATTTTCCGTGTTGTAAAAGACGGGACTTCTGTAAAAAAAACGAAAGTGGAGGTGCGGGCCCCAAGGCTTCATTACGTTCATCCTACATGTTTTGAGGCTGGCAAGCCCATGGAGTTTGTTGCCTGTGGAAGTAATTTATTACAGCCCAAATTTCGGTTTCTGGTATCCTTCTCCGGGAAATATCTGGAATATGATTATTCTCCTCCATCTTCCCATAGTCAGATTGATGGGGACACTGCTAGTAATTTAGACCATCAGTTCTTCCAGATACAGGTCCCTCAGACTGAATCAGATTGTTTTGGTCCTGCATTTATTGAGATTGAGAATGAATCTGGCTTGTCCAACTTTATACCGGTACTCATTGGGGACAAAGAGGTTTGTGCAGAAATGAAGACTATTCAGAAGAGATTTGATGAATCTCTCTCTTTGCAAGGATTACACTTTTCTTCTAGGGTTTCCCTTTCAAACTCTTGTGAAGCATCTTCAATGAGGCACACAGCATTTTCTGACATTATTTTAGATATAGCATGGTTACTAAAAAAACCCTTGTCGGAAAACTTCCAACAGATCATGACTGCTTCACAGATTCAAAGATTCAACTATCTATTGAACTTTTTGATATCTATCAAGTCAACAACCATATTGGATAAAGTTTTACAAAATCTGAAGACTCTAATGGTTAACATGGAGTTAAACAGTGCAAATAATGACAGCGTTGACGCAGATATGAGGCTATTACAGAATTACATGAAATATGCCCGTGATCTTCTTTGTAAAAAATTCGAAAGCTCTGGGGTTTCAGTCAGAGAGTCTGGAAGGTTGGTGAAAGAAGAGGATCTTGTTTCTCAAAGTCAAACCTGCTTTCAGAATGATGGAAAATCGGTTGCTCCATTCCACTGTGAGGATACGGAGATAACTGTAGATGGTAGGCTGGGTATAACTGCGGGTTCAACTGCTAATGAGAGAAGTGAAACTGTTCCGCTGTTGAGTAAAAAAGCAGTTGGTAAAGCCAACTTCATCAAGGAATTGCCTAGCGTGGCTAAGTGTTGCACTTCCGGGGAGGTCTTAAGGTCTCGCTCTAGTGGGATGTTCTTGAGATTTCGCCCTGCACTTTTTGTAATTTCTGCTACAGCTATCTGTGTAGGGTTATGTGCAGTCCTCATCCATCCTCACAAGGTTGGCGAGTTTGCAGTATCAATACGCAGATGTTTATTCGACAAATTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006355 GO:0006950 GO:0007154 GO:0008150 GO:0009267 GO:0009605 GO:0009889 GO:0009987 GO:0009991 GO:0010468 GO:0010556 GO:0016604 GO:0016607 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0033554 GO:0035874 GO:0040008 GO:0042594 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046983 GO:0048638 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0071496 GO:0080090 GO:0120126 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SBP

Protein Analysis

823

Amino Acids

92.18

Weight (kDa)

6.38

Isoelectric Point (pI)

56.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SBP	PF03110	142 - 216	1.2e-27	SBP domain
Ig_SPL7	PF26102	431 - 546	6.9e-39	SPL7-like, immunoglobulin-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009955)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G18830 AT5G18830 AT5G18830
fragaria_vesca	FvH4_4g16910 FvH4_4g16910 FvH4_4g16910 FvH4_4g16910
malus_domestica	MD13G1175800.v1.1 MD16G1177600.v1.1
prunus_persica	Prupe.1G144600_v2.0.a1
pyrus_communis	pycom13g15150 pycom16g14900
rosa_chinensis	RchiOBHm_Chr4g0420491
rosa_laevigata	RLG00000007757
rosa_roxburghii	Rroxscaffold_5G00363110
rosa_rugosa	Rorug04G0164300
rosa_samantha	Rh4AG225900 Rh4BG228600 Rh4CG240200 Rh4DG224300
rosa_wichuraiana	Rw4G019500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	234
AasI	GACNNNNNNGTC	1 cut(s)	203
Acc36I	ACCTGC	2 cut(s)	234, 2108
AccB1I	GGYRCC	1 cut(s)	1029
AccB7I	CCANNNNNTGG	1 cut(s)	120
AccI	GTMKAC	1 cut(s)	153
AccII	CGCG	1 cut(s)	338
AccIII	TCCGGA	1 cut(s)	193
AclWI	GGATC	4 cut(s)	230, 243, 1148, 2085
AcsI	RAATTY	7 cut(s)	81, 241, 1010, 1175, 1391, 2021, 2462
AcuI	CTGAAG	3 cut(s)	482, 723, 1928
AcyI	GRCGYC	1 cut(s)	444
AdeI	CACNNNGTG	1 cut(s)	487
AfaI	GTAC	3 cut(s)	1096, 1188, 1599
AfeI	AGCGCT	1 cut(s)	542
AflII	CTTAAG	1 cut(s)	2308
AflIII	ACRYGT	1 cut(s)	1328
AgeI	ACCGGT	1 cut(s)	1594
AgsI	TTSAA	9 cut(s)	86, 985, 1151, 1707, 1728, 1832, 1840, 1852, 2193
AjnI	CCWGG	1 cut(s)	1189
AluBI	AGCT	7 cut(s)	352, 460, 603, 948, 988, 2031, 2375
AluI	AGCT	7 cut(s)	352, 460, 603, 948, 988, 2031, 2375
Alw21I	GWGCWC	1 cut(s)	225
Alw26I	GTCTC	3 cut(s)	701, 792, 2319
AlwI	GGATC	4 cut(s)	230, 243, 1148, 2085
AlwNI	CAGNNNCTG	1 cut(s)	824
Ama87I	CYCGRG	2 cut(s)	274, 326
Aor13HI	TCCGGA	1 cut(s)	193
Aor51HI	AGCGCT	1 cut(s)	542
AoxI	GGCC	5 cut(s)	208, 213, 402, 417, 1299
ApaI	GGGCCC	2 cut(s)	406, 1303
ApeKI	GCWGC	2 cut(s)	43, 94
ApoI	RAATTY	7 cut(s)	81, 241, 1010, 1175, 1391, 2021, 2462
AsiGI	ACCGGT	1 cut(s)	1594
Asp700I	GAANNNNTTC	1 cut(s)	2217
AspA2I	CCTAGG	1 cut(s)	62
AspLEI	GCGC	1 cut(s)	543
AsuC2I	CCSGG	8 cut(s)	212, 218, 370, 421, 1089, 1207, 1417, 2299
AsuHPI	GGTGA	4 cut(s)	139, 542, 887, 2077
AsuII	TTCGAA	2 cut(s)	1041, 2025
AvaI	CYCGRG	2 cut(s)	274, 326
AvaII	GGWCC	5 cut(s)	196, 271, 430, 1516, 1544
AvrII	CCTAGG	1 cut(s)	62
BaeGI	GKGCMC	4 cut(s)	326, 375, 406, 1303
BamHI	GGATCC	1 cut(s)	235
BanI	GGYRCC	1 cut(s)	1029
BanII	GRGCYC	3 cut(s)	182, 406, 1303
BarI	GAAGNNNNNNTAC	2 cut(s)	726, 758
BauI	CACGAG	1 cut(s)	172
BbsI	GAAGAC	3 cut(s)	1027, 1640, 1916
Bbv12I	GWGCWC	1 cut(s)	225
BbvI	GCAGC	2 cut(s)	55, 81
BccI	CCATC	9 cut(s)	70, 340, 713, 1456, 1463, 1503, 2108, 2159, 2415
BceAI	ACGGC	3 cut(s)	56, 432, 653
BciT130I	CCWGG	1 cut(s)	1191
BciVI	GTATCC	3 cut(s)	201, 1417, 2140
BcnI	CCSGG	8 cut(s)	212, 218, 370, 421, 1089, 1207, 1417, 2299
BcoDI	GTCTC	3 cut(s)	701, 792, 2319
BfaI	CTAG	7 cut(s)	63, 651, 1017, 1482, 1692, 2274, 2322
BfmI	CTRYAG	2 cut(s)	2160, 2370
BfoI	RGCGCY	1 cut(s)	544
BfrI	CTTAAG	1 cut(s)	2308
BfuAI	ACCTGC	2 cut(s)	234, 2108
BfuI	GTATCC	3 cut(s)	201, 1417, 2140
BglII	AGATCT	1 cut(s)	133
BisI	GCNGC	4 cut(s)	44, 92, 95, 442
BlnI	CCTAGG	1 cut(s)	62
BlsI	GCNGC	4 cut(s)	45, 93, 96, 443
Bme1390I	CCNGG	9 cut(s)	212, 218, 370, 421, 1089, 1191, 1207, 1417, 2299
Bme18I	GGWCC	5 cut(s)	196, 271, 430, 1516, 1544
BmeT110I	CYCGRG	2 cut(s)	274, 326
BmrFI	CCNGG	9 cut(s)	212, 218, 370, 421, 1089, 1191, 1207, 1417, 2299
BmsI	GCATC	2 cut(s)	880, 1730
BpiI	GAAGAC	3 cut(s)	1027, 1640, 1916
BpmI	CTGGAG	3 cut(s)	251, 374, 803
Bpu14I	TTCGAA	2 cut(s)	1041, 2025
BpuEI	CTTGAG	2 cut(s)	774, 2356
BpuMI	CCSGG	8 cut(s)	212, 218, 370, 421, 1089, 1207, 1417, 2299
BsaBI	GATNNNNATC	2 cut(s)	999, 1865
BsaHI	GRCGYC	1 cut(s)	444
BsaI	GGTCTC	2 cut(s)	701, 2319
BsaJI	CCNNGG	8 cut(s)	62, 216, 275, 1087, 1190, 1304, 1350, 2298
BsaWI	WCCGGW	2 cut(s)	193, 1594
BsaXI	ACNNNNNCTCC	2 cut(s)	95, 125
Bse118I	RCCGGY	2 cut(s)	1060, 1594
Bse1I	ACTGG	1 cut(s)	1053
Bse3DI	GCAATG	1 cut(s)	1125
Bse8I	GATNNNNATC	2 cut(s)	999, 1865
BseAI	TCCGGA	1 cut(s)	193
BseBI	CCWGG	1 cut(s)	1191
BseDI	CCNNGG	8 cut(s)	62, 216, 275, 1087, 1190, 1304, 1350, 2298
BseGI	GGATG	7 cut(s)	895, 1097, 1143, 1321, 2334, 2403, 2407
BseJI	GATNNNNATC	2 cut(s)	999, 1865
BseMI	GCAATG	1 cut(s)	1125
BseMII	CTCAG	1 cut(s)	1535
BseNI	ACTGG	1 cut(s)	1053
BseRI	GAGGAG	3 cut(s)	371, 374, 1434
BseSI	GKGCMC	4 cut(s)	326, 375, 406, 1303
BseXI	GCAGC	2 cut(s)	55, 81
BseYI	CCCAGC	1 cut(s)	2173
BsgI	GTGCAG	3 cut(s)	1644, 2334, 2415
Bsh1236I	CGCG	1 cut(s)	338
BshFI	GGCC	5 cut(s)	210, 215, 404, 419, 1301
BshNI	GGYRCC	1 cut(s)	1029
BshTI	ACCGGT	1 cut(s)	1594
BsiHKAI	GWGCWC	1 cut(s)	225
BsiHKCI	CYCGRG	2 cut(s)	274, 326
BslFI	GGGAC	6 cut(s)	284, 416, 1279, 1487, 1502, 1622
BsmAI	GTCTC	3 cut(s)	701, 792, 2319
BsmFI	GGGAC	6 cut(s)	284, 416, 1279, 1487, 1502, 1622
BsnI	GGCC	5 cut(s)	210, 215, 404, 419, 1301
Bso31I	GGTCTC	2 cut(s)	701, 2319
BsoBI	CYCGRG	2 cut(s)	274, 326
Bsp119I	TTCGAA	2 cut(s)	1041, 2025
Bsp120I	GGGCCC	2 cut(s)	402, 1299
Bsp1286I	GDGCHC	6 cut(s)	182, 225, 326, 375, 406, 1303
Bsp13I	TCCGGA	1 cut(s)	193
Bsp143I	GATC	8 cut(s)	133, 168, 235, 330, 1153, 1809, 2005, 2077
Bsp19I	CCATGG	1 cut(s)	1350
BspANI	GGCC	5 cut(s)	210, 215, 404, 419, 1301
BspCNI	CTCAG	1 cut(s)	1534
BspEI	TCCGGA	1 cut(s)	193
BspFNI	CGCG	1 cut(s)	338
BspHI	TCATGA	1 cut(s)	1812
BspMI	ACCTGC	2 cut(s)	234, 2108
BspPI	GGATC	4 cut(s)	230, 243, 1148, 2085
BspT104I	TTCGAA	2 cut(s)	1041, 2025
BspT107I	GGYRCC	1 cut(s)	1029
BspTI	CTTAAG	1 cut(s)	2308
BspTNI	GGTCTC	2 cut(s)	701, 2319
BsrDI	GCAATG	1 cut(s)	1125
BsrFI	RCCGGY	2 cut(s)	1060, 1594
BsrI	ACTGG	1 cut(s)	1053
BssAI	RCCGGY	2 cut(s)	1060, 1594
BssECI	CCNNGG	8 cut(s)	62, 216, 275, 1087, 1190, 1304, 1350, 2298
BssMI	GATC	8 cut(s)	133, 168, 235, 330, 1153, 1809, 2005, 2077
BssNI	GRCGYC	1 cut(s)	444
BssSI	CACGAG	1 cut(s)	172
BssT1I	CCWWGG	3 cut(s)	62, 1304, 1350
Bst2BI	CACGAG	1 cut(s)	172
Bst2UI	CCWGG	1 cut(s)	1191
Bst4CI	ACNGT	9 cut(s)	118, 151, 517, 530, 747, 1940, 2141, 2161, 2218
Bst6I	CTCTTC	5 cut(s)	143, 699, 958, 1640, 2067
BstACI	GRCGYC	1 cut(s)	444
BstAFI	CTTAAG	1 cut(s)	2308
BstBI	TTCGAA	2 cut(s)	1041, 2025
BstC8I	GCNNGC	9 cut(s)	11, 282, 286, 322, 511, 539, 1299, 1342, 1346
BstDEI	CTNAG	2 cut(s)	1521, 2283
BstDSI	CCRYGG	1 cut(s)	1350
BstENI	CCTNNNNNAGG	1 cut(s)	230
BstF5I	GGATG	7 cut(s)	895, 1097, 1143, 1321, 2334, 2403, 2407
BstFNI	CGCG	1 cut(s)	338
BstH2I	RGCGCY	1 cut(s)	544
BstHHI	GCGC	1 cut(s)	543
BstKTI	GATC	8 cut(s)	136, 171, 238, 333, 1156, 1812, 2008, 2080
BstMAI	GTCTC	3 cut(s)	701, 792, 2319
BstMBI	GATC	8 cut(s)	133, 168, 235, 330, 1153, 1809, 2005, 2077
BstMWI	GCNNNNNNNGC	2 cut(s)	10, 43
BstNI	CCWGG	1 cut(s)	1191
BstNSI	RCATGY	1 cut(s)	1332
BstSCI	CCNGG	9 cut(s)	210, 216, 368, 419, 1087, 1189, 1205, 1415, 2297
BstSFI	CTRYAG	2 cut(s)	2160, 2370
BstSLI	GKGCMC	4 cut(s)	326, 375, 406, 1303
BstUI	CGCG	1 cut(s)	338
BstV1I	GCAGC	2 cut(s)	55, 81
BstV2I	GAAGAC	3 cut(s)	1027, 1640, 1916
BstX2I	RGATCY	4 cut(s)	133, 235, 1153, 2077
BstXI	CCANNNNNNTGG	1 cut(s)	298
BstYI	RGATCY	4 cut(s)	133, 235, 1153, 2077
BsuI	GTATCC	3 cut(s)	201, 1417, 2140
BsuRI	GGCC	5 cut(s)	210, 215, 404, 419, 1301
BtgI	CCRYGG	1 cut(s)	1350
BtsCI	GGATG	7 cut(s)	895, 1097, 1143, 1321, 2334, 2403, 2407
BtsI	GCAGTG	2 cut(s)	560, 1476
BtsIMutI	CAGTG	6 cut(s)	513, 560, 825, 1476, 1945, 2137
BveI	ACCTGC	2 cut(s)	234, 2108
Cac8I	GCNNGC	9 cut(s)	11, 282, 286, 322, 511, 539, 1299, 1342, 1346
CaiI	CAGNNNCTG	1 cut(s)	824
CciI	TCATGA	1 cut(s)	1812
CfoI	GCGC	1 cut(s)	543
Cfr10I	RCCGGY	2 cut(s)	1060, 1594
CseI	GACGC	4 cut(s)	344, 433, 611, 1970
Csp6I	GTAC	3 cut(s)	1095, 1187, 1598
CspAI	ACCGGT	1 cut(s)	1594
CviQI	GTAC	3 cut(s)	1095, 1187, 1598
DdeI	CTNAG	2 cut(s)	1521, 2283
DpnI	GATC	8 cut(s)	135, 170, 237, 332, 1155, 1811, 2007, 2079
DpnII	GATC	8 cut(s)	133, 168, 235, 330, 1153, 1809, 2005, 2077
DraIII	CACNNNGTG	1 cut(s)	487
DrdI	GACNNNNNNGTC	1 cut(s)	203
DseDI	GACNNNNNNGTC	1 cut(s)	203
Eam1104I	CTCTTC	5 cut(s)	143, 699, 958, 1640, 2067
EarI	CTCTTC	5 cut(s)	143, 699, 958, 1640, 2067
EciI	GGCGGA	2 cut(s)	172, 1073
Eco130I	CCWWGG	3 cut(s)	62, 1304, 1350
Eco24I	GRGCYC	3 cut(s)	182, 406, 1303
Eco31I	GGTCTC	2 cut(s)	701, 2319
Eco32I	GATATC	3 cut(s)	451, 523, 1863
Eco47I	GGWCC	5 cut(s)	196, 271, 430, 1516, 1544
Eco47III	AGCGCT	1 cut(s)	542
Eco57I	CTGAAG	3 cut(s)	482, 723, 1928
Eco88I	CYCGRG	2 cut(s)	274, 326
EcoNI	CCTNNNNNAGG	1 cut(s)	230
EcoO109I	RGGNCCY	5 cut(s)	271, 403, 430, 1300, 1516
EcoRI	GAATTC	1 cut(s)	241
EcoRII	CCWGG	1 cut(s)	1189
EcoRV	GATATC	3 cut(s)	451, 523, 1863
EcoT14I	CCWWGG	3 cut(s)	62, 1304, 1350
EcoT38I	GRGCYC	3 cut(s)	182, 406, 1303
ErhI	CCWWGG	3 cut(s)	62, 1304, 1350
FalI	AAGNNNNNCTT	4 cut(s)	937, 969, 2064, 2096
FaqI	GGGAC	6 cut(s)	284, 416, 1279, 1487, 1502, 1622
FauI	CCCGC	6 cut(s)	18, 261, 295, 378, 1290, 2179
FblI	GTMKAC	1 cut(s)	153
Fnu4HI	GCNGC	4 cut(s)	44, 92, 95, 442
FokI	GGATG	7 cut(s)	902, 1104, 1150, 1308, 2341, 2390, 2394
FriOI	GRGCYC	3 cut(s)	182, 406, 1303
Fsp4HI	GCNGC	4 cut(s)	44, 92, 95, 442
FspBI	CTAG	7 cut(s)	63, 651, 1017, 1482, 1692, 2274, 2322
GlaI	GCGC	1 cut(s)	542
GluI	GCNGC	4 cut(s)	44, 92, 95, 442
GsaI	CCCAGC	1 cut(s)	2177
GsuI	CTGGAG	3 cut(s)	251, 374, 803
HaeII	RGCGCY	1 cut(s)	544
HaeIII	GGCC	5 cut(s)	210, 215, 404, 419, 1301
HgaI	GACGC	4 cut(s)	344, 433, 611, 1970
HhaI	GCGC	1 cut(s)	543
Hin1I	GRCGYC	1 cut(s)	444
Hin6I	GCGC	1 cut(s)	541
HinP1I	GCGC	1 cut(s)	541
HincII	GTYRAC	4 cut(s)	154, 1875, 1924, 1960
HindII	GTYRAC	4 cut(s)	154, 1875, 1924, 1960
HpaI	GTTAAC	1 cut(s)	1924
HphI	GGTGA	4 cut(s)	139, 542, 887, 2077
Hpy166II	GTNNAC	5 cut(s)	154, 743, 1875, 1924, 1960
Hpy188III	TCNNGA	8 cut(s)	194, 391, 1102, 1427, 1507, 1813, 2055, 2335
Hpy8I	GTNNAC	5 cut(s)	154, 743, 1875, 1924, 1960
Hpy99I	CGWCG	6 cut(s)	107, 155, 158, 338, 449, 530
HpyAV	CCTTC	6 cut(s)	457, 584, 636, 763, 1420, 2052
HpyCH4III	ACNGT	9 cut(s)	118, 151, 517, 530, 747, 1940, 2141, 2161, 2218
HpyCH4IV	ACGT	1 cut(s)	1317
HpyF10VI	GCNNNNNNNGC	2 cut(s)	10, 43
HpyF3I	CTNAG	2 cut(s)	1521, 2283
HpySE526I	ACGT	1 cut(s)	1317
Hsp92I	GRCGYC	1 cut(s)	444
HspAI	GCGC	1 cut(s)	541
KflI	GGGWCCC	1 cut(s)	271
Kpn2I	TCCGGA	1 cut(s)	193
KspAI	GTTAAC	1 cut(s)	1924
Kzo9I	GATC	8 cut(s)	133, 168, 235, 330, 1153, 1809, 2005, 2077
LmnI	GCTCC	8 cut(s)	185, 220, 349, 457, 600, 665, 725, 2134
Lsp1109I	GCAGC	2 cut(s)	55, 81
LweI	GCATC	2 cut(s)	880, 1730
MaeI	CTAG	7 cut(s)	63, 651, 1017, 1482, 1692, 2274, 2322
MaeII	ACGT	1 cut(s)	1317
MaeIII	GTNAC	3 cut(s)	845, 935, 1773
MalI	GATC	8 cut(s)	135, 170, 237, 332, 1155, 1811, 2007, 2079
MboI	GATC	8 cut(s)	133, 168, 235, 330, 1153, 1809, 2005, 2077
MflI	RGATCY	4 cut(s)	133, 235, 1153, 2077
MhlI	GDGCHC	6 cut(s)	182, 225, 326, 375, 406, 1303
MlyI	GAGTC	4 cut(s)	262, 794, 1906, 2060
MmeI	TCCRAC	8 cut(s)	50, 126, 236, 989, 1050, 1608, 1773, 1828
MroI	TCCGGA	1 cut(s)	193
MroXI	GAANNNNTTC	1 cut(s)	2217
MseI	TTAA	5 cut(s)	749, 761, 1923, 1934, 2309
MspA1I	CMGCKG	3 cut(s)	94, 852, 2224
MspCI	CTTAAG	1 cut(s)	2308
MspR9I	CCNGG	9 cut(s)	212, 218, 370, 421, 1089, 1191, 1207, 1417, 2299
MvaI	CCWGG	1 cut(s)	1191
MvnI	CGCG	1 cut(s)	338
MwoI	GCNNNNNNNGC	2 cut(s)	10, 43
NciI	CCSGG	8 cut(s)	212, 218, 370, 421, 1089, 1207, 1417, 2299
NcoI	CCATGG	1 cut(s)	1350
NdeII	GATC	8 cut(s)	133, 168, 235, 330, 1153, 1809, 2005, 2077
NmeAIII	GCCGAG	1 cut(s)	211
NmuCI	GTSAC	2 cut(s)	845, 935
NspI	RCATGY	1 cut(s)	1332
NspV	TTCGAA	2 cut(s)	1041, 2025
PagI	TCATGA	1 cut(s)	1812
PaqCI	CACCTGC	1 cut(s)	234
PciI	ACATGT	1 cut(s)	1328
PcsI	WCGNNNNNNNCGW	1 cut(s)	147
PdmI	GAANNNNTTC	1 cut(s)	2217
PfeI	GAWTC	9 cut(s)	680, 827, 905, 1000, 1529, 1571, 1658, 1828, 1836
PflMI	CCANNNNNTGG	1 cut(s)	120
PfoI	TCCNGGA	2 cut(s)	1205, 1415
PinAI	ACCGGT	1 cut(s)	1594
PkrI	GCNGC	4 cut(s)	45, 93, 96, 443
PleI	GAGTC	4 cut(s)	261, 793, 1906, 2059
PpsI	GAGTC	4 cut(s)	261, 793, 1906, 2059
PpuMI	RGGWCCY	3 cut(s)	271, 430, 1516
PscI	ACATGT	1 cut(s)	1328
Psp5II	RGGWCCY	3 cut(s)	271, 430, 1516
Psp6I	CCWGG	1 cut(s)	1189
PspFI	CCCAGC	1 cut(s)	2173
PspGI	CCWGG	1 cut(s)	1189
PspOMI	GGGCCC	2 cut(s)	402, 1299
PspPPI	RGGWCCY	3 cut(s)	271, 430, 1516
PstNI	CAGNNNCTG	1 cut(s)	824
PsuI	RGATCY	4 cut(s)	133, 235, 1153, 2077
RsaI	GTAC	3 cut(s)	1096, 1188, 1599
RsaNI	GTAC	3 cut(s)	1095, 1187, 1598
SalI	GTCGAC	1 cut(s)	152
SaqAI	TTAA	5 cut(s)	749, 761, 1923, 1934, 2309
SatI	GCNGC	4 cut(s)	44, 92, 95, 442
Sau3AI	GATC	8 cut(s)	133, 168, 235, 330, 1153, 1809, 2005, 2077
SchI	GAGTC	4 cut(s)	262, 794, 1906, 2060
ScrFI	CCNGG	9 cut(s)	212, 218, 370, 421, 1089, 1191, 1207, 1417, 2299
SduI	GDGCHC	6 cut(s)	182, 225, 326, 375, 406, 1303
SfaNI	GCATC	2 cut(s)	880, 1730
SfcI	CTRYAG	2 cut(s)	2160, 2370
SfuI	TTCGAA	2 cut(s)	1041, 2025
SgrDI	CGTCGACG	1 cut(s)	152
SinI	GGWCC	5 cut(s)	196, 271, 430, 1516, 1544
SmlI	CTYRAG	3 cut(s)	789, 2308, 2335
SmoI	CTYRAG	3 cut(s)	789, 2308, 2335
SspI	AATATT	2 cut(s)	1038, 1219
SspMI	CTAG	7 cut(s)	63, 651, 1017, 1482, 1692, 2274, 2322
StyD4I	CCNGG	9 cut(s)	210, 216, 368, 419, 1087, 1189, 1205, 1415, 2297
StyI	CCWWGG	3 cut(s)	62, 1304, 1350
TaaI	ACNGT	9 cut(s)	118, 151, 517, 530, 747, 1940, 2141, 2161, 2218
TaiI	ACGT	1 cut(s)	1320
TatI	WGTACW	1 cut(s)	1094
TauI	GCSGC	2 cut(s)	94, 444
TfiI	GAWTC	9 cut(s)	680, 827, 905, 1000, 1529, 1571, 1658, 1828, 1836
Tru1I	TTAA	5 cut(s)	749, 761, 1923, 1934, 2309
Tru9I	TTAA	5 cut(s)	749, 761, 1923, 1934, 2309
TscAI	CASTG	6 cut(s)	520, 560, 825, 1483, 1945, 2144
TseFI	GTSAC	2 cut(s)	845, 935
TseI	GCWGC	2 cut(s)	43, 94
Tsp45I	GTSAC	2 cut(s)	845, 935
TspGWI	ACGGA	5 cut(s)	426, 830, 915, 1238, 2165
TspRI	CASTG	6 cut(s)	520, 560, 825, 1483, 1945, 2144
Van91I	CCANNNNNTGG	1 cut(s)	120
Vha464I	CTTAAG	1 cut(s)	2308
VpaK11BI	GGWCC	5 cut(s)	196, 271, 430, 1516, 1544
XagI	CCTNNNNNAGG	1 cut(s)	230
XapI	RAATTY	7 cut(s)	81, 241, 1010, 1175, 1391, 2021, 2462
XceI	RCATGY	1 cut(s)	1332
XmaJI	CCTAGG	1 cut(s)	62
XmiI	GTMKAC	1 cut(s)	153
XmnI	GAANNNNTTC	1 cut(s)	2217
XspI	CTAG	7 cut(s)	63, 651, 1017, 1482, 1692, 2274, 2322

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.