MD16G1186600.v1.1

Peroxiredoxin-2F

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0012466
Physical Location & Seq
Forward (+)
16110005 .. 16111912
1908 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1186600.v1.1.491

Sequence Viewer

Length: 606 bp
ATGGCGTCCACACTTCTGAAGCGAACGAGCTTCTCCGCAATGAAATCAGTGGTCGGAGGCTTCCAAATCGGAGCTCAATCAAGAGCCTACGCGGCCGCTGCAGTCGGGACCGACCTAGTCTCCGCCGCACCCAATGTTTCCCTCCAGAAAGCTCGCACCTGGGACGAAGGCGTCTCCTCCAACTTCGCCACCACTCCTCTCAAAGACATTTTCAAGGGCAAGAAAGTCGTCATCTTTGGGCTCCCTGGCGCCTTCACCGGCGTTTGTTCTGTGCAACACGTGCCTAGTTACAAGAAGCAGATTGATAAGTTTAAGGCCAAAGGGGTCGATTCTGTGATTTGCGTAGCTGTGAATGATCCATATGTTATGAATGGCTGGGCAACCAAACTCGAAGCCAAAGAAGCTATTGAGTTCTATGGGGACTTCGATGGGAGCTTCCACAAAAGCTTGGAATTGGATAAAGATCTCAGCGGTGCCTTGCTTGGACATCGCTCTCAAAGATGGTCAGCATATGTGGTCGACGGGAAGGTGAAAACTCTAAATGTGGAGGAAGTGCCATCAGACTTCAAGGTTTCTGGCGGGGACGTCATTTTGGGGCAGATCTAG

Protein Analysis

202

Amino Acids

21.53

Weight (kDa)

8.97

Isoelectric Point (pI)

26.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AhpC-TSA PF00578 42 - 154 4.2e-10 AhpC/TSA family
Redoxin PF08534 43 - 192 9e-24 Redoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012466)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06050
fragaria_vesca FvH4_4g14840
malus_domestica MD13G1185800.v1.1 MD16G1186600.v1.1
prunus_persica Prupe.1G153400_v2.0.a1
pyrus_communis pycom13g16030 pycom16g15640
rosa_chinensis RchiOBHm_Chr4g0417421 RchiOBHm_Chr4g0417491
rosa_laevigata RLG00000007904 RLG00000007910
rosa_multiflora Rmu_sc0004142.1_g000019
rosa_roxburghii Rroxscaffold_5G00361170
rosa_rugosa Rorug04G0148000
rosa_samantha Rh4AG211300 Rh4BG211500 Rh4CG222000 Rh4DG209000
rosa_wichuraiana Rw4G017860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 170
AatII GACGTC 1 cut(s) 588
AccB1I GGYRCC 2 cut(s) 248, 473
AccI GTMKAC 1 cut(s) 519
AccII CGCG 1 cut(s) 92
AciI CCGC 7 cut(s) 36, 92, 96, 123, 126, 471, 579
AclWI GGATC 1 cut(s) 350
AcoI YGGCCR 1 cut(s) 93
AcuI CTGAAG 1 cut(s) 38
AcvI CACGTG 1 cut(s) 280
AcyI GRCGYC 4 cut(s) 5, 171, 249, 585
AflIII ACRYGT 1 cut(s) 277
AgsI TTSAA 2 cut(s) 214, 568
AjnI CCWGG 2 cut(s) 158, 244
AluBI AGCT 7 cut(s) 30, 74, 152, 347, 404, 435, 447
AluI AGCT 7 cut(s) 30, 74, 152, 347, 404, 435, 447
Alw21I GWGCWC 1 cut(s) 76
Alw26I GTCTC 2 cut(s) 124, 178
AlwI GGATC 1 cut(s) 350
AoxI GGCC 2 cut(s) 93, 315
ApeKI GCWGC 1 cut(s) 98
AspLEI GCGC 1 cut(s) 251
AspS9I GGNCC 1 cut(s) 108
AsuHPI GGTGA 2 cut(s) 247, 541
AvaII GGWCC 1 cut(s) 108
BanI GGYRCC 2 cut(s) 248, 473
BanII GRGCYC 2 cut(s) 76, 243
BbrPI CACGTG 1 cut(s) 280
Bbv12I GWGCWC 1 cut(s) 76
BbvI GCAGC 1 cut(s) 85
BccI CCATC 3 cut(s) 422, 495, 565
BcgI CGANNNNNNTGC 2 cut(s) 208, 242
BciT130I CCWGG 2 cut(s) 160, 246
BcoDI GTCTC 2 cut(s) 124, 178
BfaI CTAG 3 cut(s) 116, 285, 604
BfmI CTRYAG 1 cut(s) 99
BfoI RGCGCY 1 cut(s) 252
BglI GCCNNNNNGGC 1 cut(s) 92
BglII AGATCT 2 cut(s) 463, 600
BisI GCNGC 4 cut(s) 93, 96, 99, 126
BlsI GCNGC 4 cut(s) 94, 97, 100, 127
Bme1390I CCNGG 2 cut(s) 160, 246
Bme18I GGWCC 1 cut(s) 108
BmgT120I GGNCC 1 cut(s) 108
BmiI GGNNCC 4 cut(s) 109, 242, 250, 475
BmrFI CCNGG 2 cut(s) 160, 246
BpmI CTGGAG 1 cut(s) 128
BsaAI YACGTR 1 cut(s) 280
BsaBI GATNNNNATC 1 cut(s) 462
BsaHI GRCGYC 4 cut(s) 5, 171, 249, 585
BsaJI CCNNGG 2 cut(s) 159, 244
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bse118I RCCGGY 1 cut(s) 257
Bse3DI GCAATG 1 cut(s) 45
Bse8I GATNNNNATC 1 cut(s) 462
BseBI CCWGG 2 cut(s) 160, 246
BseDI CCNNGG 2 cut(s) 159, 244
BseJI GATNNNNATC 1 cut(s) 462
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 1 cut(s) 481
BseRI GAGGAG 2 cut(s) 166, 186
BseX3I CGGCCG 1 cut(s) 93
BseXI GCAGC 1 cut(s) 85
BseYI CCCAGC 1 cut(s) 375
Bsh1236I CGCG 1 cut(s) 92
Bsh1285I CGRYCG 1 cut(s) 96
BshFI GGCC 2 cut(s) 95, 317
BshNI GGYRCC 2 cut(s) 248, 473
BsiEI CGRYCG 1 cut(s) 96
BsiHKAI GWGCWC 1 cut(s) 76
BsiSI CCGG 1 cut(s) 258
BslFI GGGAC 4 cut(s) 121, 176, 434, 596
BsmAI GTCTC 2 cut(s) 124, 178
BsmBI CGTCTC 1 cut(s) 178
BsmFI GGGAC 4 cut(s) 121, 176, 434, 596
BsnI GGCC 2 cut(s) 95, 317
Bsp1286I GDGCHC 2 cut(s) 76, 243
Bsp143I GATC 3 cut(s) 355, 463, 600
BspACI CCGC 7 cut(s) 36, 92, 96, 123, 126, 471, 579
BspANI GGCC 2 cut(s) 95, 317
BspCNI CTCAG 1 cut(s) 480
BspFNI CGCG 1 cut(s) 92
BspLI GGNNCC 4 cut(s) 109, 242, 250, 475
BspMAI CTGCAG 1 cut(s) 103
BspPI GGATC 1 cut(s) 350
BspT107I GGYRCC 2 cut(s) 248, 473
BsrDI GCAATG 1 cut(s) 45
BsrFI RCCGGY 1 cut(s) 257
BssAI RCCGGY 1 cut(s) 257
BssECI CCNNGG 2 cut(s) 159, 244
BssMI GATC 3 cut(s) 355, 463, 600
BssNI GRCGYC 4 cut(s) 5, 171, 249, 585
Bst2UI CCWGG 2 cut(s) 160, 246
BstACI GRCGYC 4 cut(s) 5, 171, 249, 585
BstAPI GCANNNNNTGC 1 cut(s) 280
BstBAI YACGTR 1 cut(s) 280
BstC8I GCNNGC 1 cut(s) 154
BstDEI CTNAG 1 cut(s) 467
BstFNI CGCG 1 cut(s) 92
BstH2I RGCGCY 1 cut(s) 252
BstHHI GCGC 1 cut(s) 251
BstKTI GATC 3 cut(s) 358, 466, 603
BstMAI GTCTC 2 cut(s) 124, 178
BstMBI GATC 3 cut(s) 355, 463, 600
BstMCI CGRYCG 1 cut(s) 96
BstMWI GCNNNNNNNGC 4 cut(s) 92, 98, 280, 401
BstNI CCWGG 2 cut(s) 160, 246
BstSCI CCNGG 2 cut(s) 158, 244
BstSFI CTRYAG 1 cut(s) 99
BstUI CGCG 1 cut(s) 92
BstV1I GCAGC 1 cut(s) 85
BstX2I RGATCY 2 cut(s) 463, 600
BstYI RGATCY 2 cut(s) 463, 600
BstZI CGGCCG 1 cut(s) 93
BsuRI GGCC 2 cut(s) 95, 317
BtgZI GCGATG 1 cut(s) 473
BtsIMutI CAGTG 1 cut(s) 54
Cac8I GCNNGC 1 cut(s) 154
CciNI GCGGCCGC 1 cut(s) 93
CfoI GCGC 1 cut(s) 251
Cfr10I RCCGGY 1 cut(s) 257
Cfr13I GGNCC 1 cut(s) 108
CseI GACGC 1 cut(s) 160
DdeI CTNAG 1 cut(s) 467
DinI GGCGCC 1 cut(s) 250
DpnI GATC 3 cut(s) 357, 465, 602
DpnII GATC 3 cut(s) 355, 463, 600
DrdI GACNNNNNNGTC 1 cut(s) 170
DseDI GACNNNNNNGTC 1 cut(s) 170
EaeI YGGCCR 1 cut(s) 93
EagI CGGCCG 1 cut(s) 93
EciI GGCGGA 1 cut(s) 112
Ecl136II GAGCTC 1 cut(s) 74
EclXI CGGCCG 1 cut(s) 93
Eco24I GRGCYC 2 cut(s) 76, 243
Eco47I GGWCC 1 cut(s) 108
Eco52I CGGCCG 1 cut(s) 93
Eco53kI GAGCTC 1 cut(s) 74
Eco57I CTGAAG 1 cut(s) 38
Eco72I CACGTG 1 cut(s) 280
EcoICRI GAGCTC 1 cut(s) 74
EcoRII CCWGG 2 cut(s) 158, 244
EcoT38I GRGCYC 2 cut(s) 76, 243
EgeI GGCGCC 1 cut(s) 250
EheI GGCGCC 1 cut(s) 250
Esp3I CGTCTC 1 cut(s) 178
FaiI YATR 6 cut(s) 361, 363, 368, 417, 511, 513
FaqI GGGAC 4 cut(s) 121, 176, 434, 596
FauI CCCGC 1 cut(s) 572
FauNDI CATATG 2 cut(s) 361, 511
FblI GTMKAC 1 cut(s) 519
Fnu4HI GCNGC 4 cut(s) 93, 96, 99, 126
FriOI GRGCYC 2 cut(s) 76, 243
Fsp4HI GCNGC 4 cut(s) 93, 96, 99, 126
FspBI CTAG 3 cut(s) 116, 285, 604
GlaI GCGC 1 cut(s) 250
GluI GCNGC 4 cut(s) 93, 96, 99, 126
GsaI CCCAGC 1 cut(s) 379
GsuI CTGGAG 1 cut(s) 128
HaeII RGCGCY 1 cut(s) 252
HaeIII GGCC 2 cut(s) 95, 317
HapII CCGG 1 cut(s) 258
HgaI GACGC 1 cut(s) 160
HhaI GCGC 1 cut(s) 251
Hin1I GRCGYC 4 cut(s) 5, 171, 249, 585
Hin6I GCGC 1 cut(s) 249
HinP1I GCGC 1 cut(s) 249
HincII GTYRAC 1 cut(s) 520
HindII GTYRAC 1 cut(s) 520
HindIII AAGCTT 1 cut(s) 445
HinfI GANTC 1 cut(s) 329
HpaII CCGG 1 cut(s) 258
HphI GGTGA 2 cut(s) 247, 541
Hpy166II GTNNAC 2 cut(s) 9, 520
Hpy188I TCNGA 4 cut(s) 18, 56, 71, 562
Hpy188III TCNNGA 3 cut(s) 81, 106, 145
Hpy8I GTNNAC 2 cut(s) 9, 520
Hpy99I CGWCG 1 cut(s) 524
HpyAV CCTTC 3 cut(s) 161, 262, 520
HpyCH4IV ACGT 2 cut(s) 279, 585
HpyCH4V TGCA 2 cut(s) 101, 274
HpyF10VI GCNNNNNNNGC 4 cut(s) 92, 98, 280, 401
HpyF3I CTNAG 1 cut(s) 467
HpySE526I ACGT 2 cut(s) 279, 585
Hsp92I GRCGYC 4 cut(s) 5, 171, 249, 585
HspAI GCGC 1 cut(s) 249
KasI GGCGCC 1 cut(s) 248
Kzo9I GATC 3 cut(s) 355, 463, 600
LmnI GCTCC 3 cut(s) 71, 246, 432
LpnPI CCDG 8 cut(s) 145, 158, 172, 231, 258, 271, 361, 561
Lsp1109I GCAGC 1 cut(s) 85
MaeI CTAG 3 cut(s) 116, 285, 604
MaeII ACGT 2 cut(s) 279, 585
MaeIII GTNAC 1 cut(s) 287
MalI GATC 3 cut(s) 357, 465, 602
MboI GATC 3 cut(s) 355, 463, 600
MflI RGATCY 2 cut(s) 463, 600
MhlI GDGCHC 2 cut(s) 76, 243
MluCI AATT 1 cut(s) 452
Mly113I GGCGCC 1 cut(s) 249
MmeI TCCRAC 2 cut(s) 34, 204
MnlI CCTC 5 cut(s) 50, 152, 187, 207, 541
MseI TTAA 1 cut(s) 312
MspA1I CMGCKG 2 cut(s) 98, 471
MspI CCGG 1 cut(s) 258
MspR9I CCNGG 2 cut(s) 160, 246
MvaI CCWGG 2 cut(s) 160, 246
MvnI CGCG 1 cut(s) 92
MwoI GCNNNNNNNGC 4 cut(s) 92, 98, 280, 401
NarI GGCGCC 1 cut(s) 249
NdeI CATATG 2 cut(s) 361, 511
NdeII GATC 3 cut(s) 355, 463, 600
NlaIV GGNNCC 4 cut(s) 109, 242, 250, 475
NotI GCGGCCGC 1 cut(s) 93
PfeI GAWTC 1 cut(s) 329
PflFI GACNNNGTC 1 cut(s) 116
PkrI GCNGC 4 cut(s) 94, 97, 100, 127
PluTI GGCGCC 1 cut(s) 252
PmaCI CACGTG 1 cut(s) 280
PmlI CACGTG 1 cut(s) 280
Ppu21I YACGTR 1 cut(s) 280
Psp124BI GAGCTC 1 cut(s) 76
Psp6I CCWGG 2 cut(s) 158, 244
PspCI CACGTG 1 cut(s) 280
PspFI CCCAGC 1 cut(s) 375
PspGI CCWGG 2 cut(s) 158, 244
PspN4I GGNNCC 4 cut(s) 109, 242, 250, 475
PspPI GGNCC 1 cut(s) 108
PstI CTGCAG 1 cut(s) 103
PsuI RGATCY 2 cut(s) 463, 600
PsyI GACNNNGTC 1 cut(s) 116
SacI GAGCTC 1 cut(s) 76
SalI GTCGAC 1 cut(s) 518
SaqAI TTAA 1 cut(s) 312
SatI GCNGC 4 cut(s) 93, 96, 99, 126
Sau3AI GATC 3 cut(s) 355, 463, 600
Sau96I GGNCC 1 cut(s) 108
ScrFI CCNGG 2 cut(s) 160, 246
SduI GDGCHC 2 cut(s) 76, 243
SfcI CTRYAG 1 cut(s) 99
SfoI GGCGCC 1 cut(s) 250
SgrAI CRCCGGYG 1 cut(s) 257
SinI GGWCC 1 cut(s) 108
Sse9I AATT 1 cut(s) 452
SsiI CCGC 7 cut(s) 36, 92, 96, 123, 126, 471, 579
SspDI GGCGCC 1 cut(s) 248
SspMI CTAG 3 cut(s) 116, 285, 604
SstI GAGCTC 1 cut(s) 76
StyD4I CCNGG 2 cut(s) 158, 244
TaiI ACGT 2 cut(s) 282, 588
TaqI TCGA 4 cut(s) 327, 390, 426, 519
TaqII GACCGA 1 cut(s) 125
TasI AATT 1 cut(s) 452
TauI GCSGC 3 cut(s) 95, 98, 128
TfiI GAWTC 1 cut(s) 329
Tru1I TTAA 1 cut(s) 312
Tru9I TTAA 1 cut(s) 312
TscAI CASTG 1 cut(s) 54
TseI GCWGC 1 cut(s) 98
TspDTI ATGAA 2 cut(s) 56, 383
TspRI CASTG 1 cut(s) 54
Tth111I GACNNNGTC 1 cut(s) 116
VpaK11BI GGWCC 1 cut(s) 108
XmiI GTMKAC 1 cut(s) 519
XspI CTAG 3 cut(s) 116, 285, 604
ZraI GACGTC 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.