MD16G1221400.v1.1

Pleckstrin homology domain-containing family A member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0006345
Physical Location & Seq
Forward (+)
22128297 .. 22130917
2621 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1221400.v1.1.491

Sequence Viewer

Length: 609 bp
ATGGAGGGCACTGTGTTTGCTCCCGCTTTGGAAGGATTCAAGGCTGTCAAGTCCGACCAAGGAGAGATTCTGACCAAGCCTTTCTTGGATGTCTGCAAGCAGATTTTACCTGTTATAGATAAGTTTGGAGCTGCTATGACCCTTGTTAAATCAGACATCGGAGGTAACATAACGAGATTGGAATCTAAATATGAATCCAATCCTGCCGCATTCAACCTCTTGTACAGTTTGGTACAAGTTGAGATAGAAGCGAAAACAGCAAAAGCGTCATCCAGCTGCACCAATGGTCTTCTTTGGTTGACAAGAGCAATGGATTTCTTGGTAGAGCTGTTCCGGAACTTACTGGAGCATCAAGATTGGGCAATGTCAGAGGCTTGTACAGATTCCTATGGCAAGACCCTGAAAAAATGGCATGGATGGATTGCTAGTTCAACTTTCAGTATTGCCATGAAGCTTGCTCCTGACAGAAAGAAGTTCATGGAGGTGATAGGAGGCAACAGCGATGTCATGGGTGATATAGAGAAATTCTGTGATGCCTTTACGCCTCTTCTTCAAGAGAATCACAAGTTTTTGACTAGTGTTGGCATGGATGATTTGAAGGCTTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0016020 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GLTP

Protein Analysis

203

Amino Acids

22.36

Weight (kDa)

5.45

Isoelectric Point (pI)

43.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GLTP PF08718 23 - 164 9.3e-49 Glycolipid transfer protein (GLTP)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 333
AciI CCGC 2 cut(s) 24, 207
AcsI RAATTY 1 cut(s) 524
AfaI GTAC 3 cut(s) 224, 234, 379
AgsI TTSAA 5 cut(s) 40, 214, 432, 554, 598
AhlI ACTAGT 1 cut(s) 575
AjuI GAANNNNNNNTTGG 2 cut(s) 51, 83
AloI GAACNNNNNNTCC 2 cut(s) 412, 444
AluBI AGCT 4 cut(s) 131, 276, 328, 454
AluI AGCT 4 cut(s) 131, 276, 328, 454
Aor13HI TCCGGA 1 cut(s) 333
ApeKI GCWGC 2 cut(s) 131, 276
ApoI RAATTY 1 cut(s) 524
AsuHPI GGTGA 2 cut(s) 496, 524
BaeGI GKGCMC 1 cut(s) 11
BbsI GAAGAC 1 cut(s) 281
BbvI GCAGC 2 cut(s) 118, 263
BccI CCATC 1 cut(s) 411
BcuI ACTAGT 1 cut(s) 575
BfaI CTAG 2 cut(s) 426, 576
BisI GCNGC 3 cut(s) 132, 207, 277
BlsI GCNGC 3 cut(s) 133, 208, 278
BmsI GCATC 2 cut(s) 358, 523
BpiI GAAGAC 1 cut(s) 281
BpmI CTGGAG 1 cut(s) 365
BsaBI GATNNNNATC 1 cut(s) 181
BsaJI CCNNGG 1 cut(s) 58
BsaWI WCCGGW 1 cut(s) 333
Bse1I ACTGG 1 cut(s) 348
Bse3DI GCAATG 2 cut(s) 315, 369
Bse8I GATNNNNATC 1 cut(s) 181
BseAI TCCGGA 1 cut(s) 333
BseDI CCNNGG 1 cut(s) 58
BseGI GGATG 4 cut(s) 94, 269, 422, 595
BseJI GATNNNNATC 1 cut(s) 181
BseMI GCAATG 2 cut(s) 315, 369
BseNI ACTGG 1 cut(s) 348
BseSI GKGCMC 1 cut(s) 11
BseXI GCAGC 2 cut(s) 118, 263
BsgI GTGCAG 1 cut(s) 262
BsiSI CCGG 1 cut(s) 334
BsmI GAATGC 1 cut(s) 209
Bsp1286I GDGCHC 1 cut(s) 11
Bsp13I TCCGGA 1 cut(s) 333
Bsp1407I TGTACA 2 cut(s) 222, 377
BspACI CCGC 2 cut(s) 24, 207
BspEI TCCGGA 1 cut(s) 333
BsrDI GCAATG 2 cut(s) 315, 369
BsrGI TGTACA 2 cut(s) 222, 377
BsrI ACTGG 1 cut(s) 348
BssECI CCNNGG 1 cut(s) 58
BssT1I CCWWGG 1 cut(s) 58
Bst4CI ACNGT 2 cut(s) 13, 227
Bst6I CTCTTC 1 cut(s) 552
BstAUI TGTACA 2 cut(s) 222, 377
BstC8I GCNNGC 2 cut(s) 98, 456
BstF5I GGATG 4 cut(s) 94, 269, 422, 595
BstMWI GCNNNNNNNGC 1 cut(s) 257
BstSLI GKGCMC 1 cut(s) 11
BstV1I GCAGC 2 cut(s) 118, 263
BstV2I GAAGAC 1 cut(s) 281
BtgZI GCGATG 1 cut(s) 516
BtsCI GGATG 4 cut(s) 94, 269, 422, 595
BtsIMutI CAGTG 1 cut(s) 9
Cac8I GCNNGC 2 cut(s) 98, 456
CseI GACGC 1 cut(s) 255
Csp6I GTAC 3 cut(s) 223, 233, 378
CviAII CATG 5 cut(s) 413, 448, 478, 508, 586
CviJI RGCY 8 cut(s) 44, 79, 131, 276, 328, 374, 454, 602
CviKI_1 RGCY 8 cut(s) 44, 79, 131, 276, 328, 374, 454, 602
CviQI GTAC 3 cut(s) 223, 233, 378
Eam1104I CTCTTC 1 cut(s) 552
EarI CTCTTC 1 cut(s) 552
Eco130I CCWWGG 1 cut(s) 58
EcoT14I CCWWGG 1 cut(s) 58
ErhI CCWWGG 1 cut(s) 58
FaeI CATG 5 cut(s) 416, 451, 481, 511, 589
FalI AAGNNNNNCTT 2 cut(s) 68, 100
FatI CATG 5 cut(s) 412, 447, 477, 507, 585
FauI CCCGC 1 cut(s) 31
Fnu4HI GCNGC 3 cut(s) 132, 207, 277
FokI GGATG 4 cut(s) 101, 256, 429, 602
Fsp4HI GCNGC 3 cut(s) 132, 207, 277
FspBI CTAG 2 cut(s) 426, 576
GluI GCNGC 3 cut(s) 132, 207, 277
GsuI CTGGAG 1 cut(s) 365
HapII CCGG 1 cut(s) 334
HgaI GACGC 1 cut(s) 255
Hin1II CATG 5 cut(s) 416, 451, 481, 511, 589
HincII GTYRAC 1 cut(s) 300
HindII GTYRAC 1 cut(s) 300
HindIII AAGCTT 1 cut(s) 452
HinfI GANTC 6 cut(s) 36, 67, 182, 194, 383, 559
HpaII CCGG 1 cut(s) 334
HphI GGTGA 2 cut(s) 496, 524
Hpy166II GTNNAC 1 cut(s) 300
Hpy188I TCNGA 5 cut(s) 55, 72, 154, 161, 370
Hpy188III TCNNGA 4 cut(s) 334, 353, 461, 554
Hpy8I GTNNAC 1 cut(s) 300
HpyAV CCTTC 2 cut(s) 26, 592
HpyCH4III ACNGT 2 cut(s) 13, 227
HpyCH4V TGCA 2 cut(s) 96, 279
HpyF10VI GCNNNNNNNGC 1 cut(s) 257
Hsp92II CATG 5 cut(s) 416, 451, 481, 511, 589
Kpn2I TCCGGA 1 cut(s) 333
LmnI GCTCC 4 cut(s) 25, 128, 346, 463
LpnPI CCDG 7 cut(s) 123, 216, 286, 329, 347, 413, 474
Lsp1109I GCAGC 2 cut(s) 118, 263
LweI GCATC 2 cut(s) 358, 523
MaeI CTAG 2 cut(s) 426, 576
MaeIII GTNAC 1 cut(s) 164
MboII GAAGA 3 cut(s) 281, 539, 542
MhlI GDGCHC 1 cut(s) 11
MluCI AATT 1 cut(s) 524
MmeI TCCRAC 1 cut(s) 78
MnlI CCTC 6 cut(s) 155, 227, 364, 475, 485, 555
MroI TCCGGA 1 cut(s) 333
MseI TTAA 1 cut(s) 147
MslI CAYNNNNRTG 1 cut(s) 482
MspA1I CMGCKG 1 cut(s) 276
MspI CCGG 1 cut(s) 334
Mva1269I GAATGC 1 cut(s) 209
MwoI GCNNNNNNNGC 1 cut(s) 257
NlaIII CATG 5 cut(s) 416, 451, 481, 511, 589
PctI GAATGC 1 cut(s) 209
PfeI GAWTC 6 cut(s) 36, 67, 182, 194, 383, 559
PkrI GCNGC 3 cut(s) 133, 208, 278
PvuII CAGCTG 1 cut(s) 276
RsaI GTAC 3 cut(s) 224, 234, 379
RsaNI GTAC 3 cut(s) 223, 233, 378
RseI CAYNNNNRTG 1 cut(s) 482
SaqAI TTAA 1 cut(s) 147
SatI GCNGC 3 cut(s) 132, 207, 277
SduI GDGCHC 1 cut(s) 11
SetI ASST 8 cut(s) 112, 133, 166, 219, 278, 330, 456, 486
SfaNI GCATC 2 cut(s) 358, 523
SmiMI CAYNNNNRTG 1 cut(s) 482
SpeI ACTAGT 1 cut(s) 575
Sse9I AATT 1 cut(s) 524
SsiI CCGC 2 cut(s) 24, 207
SspMI CTAG 2 cut(s) 426, 576
StyI CCWWGG 1 cut(s) 58
TaaI ACNGT 2 cut(s) 13, 227
TasI AATT 1 cut(s) 524
TatI WGTACW 2 cut(s) 222, 377
TauI GCSGC 1 cut(s) 209
TfiI GAWTC 6 cut(s) 36, 67, 182, 194, 383, 559
Tru1I TTAA 1 cut(s) 147
Tru9I TTAA 1 cut(s) 147
TscAI CASTG 1 cut(s) 16
TseI GCWGC 2 cut(s) 131, 276
TspDTI ATGAA 3 cut(s) 207, 464, 466
TspRI CASTG 1 cut(s) 16
XapI RAATTY 1 cut(s) 524
XcmI CCANNNNNNNNNTGG 1 cut(s) 82
XspI CTAG 2 cut(s) 426, 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.