MD16G1224700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0011906
Physical Location & Seq
Forward (+)
22684324 .. 22685854
1531 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1224700.v1.1.491

Sequence Viewer

Length: 828 bp
ATGGGGTGTTTTCTTGCCTGTTTCGGTTTCTCTAAGAAGAAAAAGCGGCGAAAACCGGCTACTAAAGCCGCCGGTGGTGACCATAGACGTGTGAGTTATGTACCACTCGATTCATCTGTCACAATTATTGGTATTGATGGTGCAAAAGAATGTTCGAAATCTGAACTATGCAGAGACAAGGCAAAGGAGCAGACAAGGTTGAAAACCCGAAAGAAAGTTAGCTTCAATCCGAATGTGCAGACCTATGAGCCTATTTCGACCGATTACCGTTCCTTGGAGAGCTATGAGGAGGAAGACTTGGAAGAAAAGAATGTCAAAAGCCTTTCCACTTCAGCATCTAAAAGGGATTCAACTACATTGAGATTGGGACTGTTTCCCTCAAACTACAGGTACCAAAATGTACGGGACAGCTACGAAGAAGAGGAAGGCGATTCAGATGACAATGATATTGACGTTGATGATGAGGATGACTACTATGATGACGATGAAGAGAGTGATATCGATGAACAACGAACAAGCCGAGAAGGGTTTTCGGAGGAACTATGTTCTGCCTCTAGAAATGGAGAATCGGAGTATCCAAATGCTAGAGATTATAAGCATGTGCATTCTGTACTGAGTCCAGTAGAGAATCTCATACAGTGGAAAGCAGCGAAAGCGAAAACAGCAGCACCACAAAAGCACCAAAAGGAGAATATTGCAATATTTCAACCACCCCAGATGCCTTATAGCAGCAGGTCGAATGTTAACCGGTCCACGAAACCTCTACTGCAACAAATCCCAGTTCATGCCAGTCTTTCGAGTTGGTTAAATTCGCAAAGTAGTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

276

Amino Acids

31.27

Weight (kDa)

5.45

Isoelectric Point (pI)

63.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011906)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33400
fragaria_vesca FvH4_4g04330 FvH4_4g04330
malus_domestica MD13G1219300.v1.1 MD16G1224700.v1.1
prunus_persica Prupe.1G046000_v2.0.a1
pyrus_communis pycom13g19420 pycom16g18800
rosa_chinensis RchiOBHm_Chr4g0394781
rosa_laevigata RLG00000009661
rosa_multiflora Rmu_sc0005356.1_g000002
rosa_roxburghii Rroxscaffold_5G00339820
rosa_rugosa Rorug03G0361500.1
rosa_samantha Rh3BG335900 Rh4AG055100 Rh4BG053000 Rh4CG059900 Rh4DG051600
rosa_wichuraiana Rw4G004400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 594
Acc36I ACCTGC 1 cut(s) 723
Acc65I GGTACC 1 cut(s) 390
AccB1I GGYRCC 1 cut(s) 390
AciI CCGC 2 cut(s) 46, 69
AcsI RAATTY 1 cut(s) 808
AcuI CTGAAG 1 cut(s) 315
AfaI GTAC 4 cut(s) 102, 392, 402, 612
AfiI CCNNNNNNNGG 1 cut(s) 274
AflIII ACRYGT 1 cut(s) 88
AgeI ACCGGT 1 cut(s) 747
AgsI TTSAA 4 cut(s) 202, 226, 351, 707
AjiI CACGTC 1 cut(s) 89
AluBI AGCT 3 cut(s) 222, 282, 411
AluI AGCT 3 cut(s) 222, 282, 411
Alw26I GTCTC 1 cut(s) 168
ApeKI GCWGC 3 cut(s) 647, 665, 729
ApoI RAATTY 1 cut(s) 808
AsiGI ACCGGT 1 cut(s) 747
Asp718I GGTACC 1 cut(s) 390
AspS9I GGNCC 1 cut(s) 750
AsuHPI GGTGA 1 cut(s) 89
AsuII TTCGAA 1 cut(s) 155
AvaII GGWCC 1 cut(s) 750
BanI GGYRCC 1 cut(s) 390
BbsI GAAGAC 1 cut(s) 300
BbvI GCAGC 3 cut(s) 659, 677, 741
BccI CCATC 1 cut(s) 131
BciVI GTATCC 1 cut(s) 585
BcoDI GTCTC 1 cut(s) 168
BfaI CTAG 2 cut(s) 555, 585
BfmI CTRYAG 1 cut(s) 385
BfuAI ACCTGC 1 cut(s) 723
BfuI GTATCC 1 cut(s) 585
BisI GCNGC 5 cut(s) 47, 69, 648, 666, 730
BlsI GCNGC 5 cut(s) 48, 70, 649, 667, 731
Bme18I GGWCC 1 cut(s) 750
BmgBI CACGTC 1 cut(s) 89
BmgT120I GGNCC 1 cut(s) 750
BmiI GGNNCC 1 cut(s) 392
BmrI ACTGGG 1 cut(s) 773
BmsI GCATC 2 cut(s) 344, 708
BmuI ACTGGG 1 cut(s) 773
BpiI GAAGAC 1 cut(s) 300
Bpu14I TTCGAA 1 cut(s) 155
Bsa29I ATCGAT 1 cut(s) 501
BsaJI CCNNGG 1 cut(s) 273
BsaWI WCCGGW 1 cut(s) 747
Bsc4I CCNNNNNNNGG 1 cut(s) 274
Bse118I RCCGGY 3 cut(s) 55, 71, 747
Bse1I ACTGG 3 cut(s) 620, 779, 789
BseCI ATCGAT 1 cut(s) 501
BseDI CCNNGG 1 cut(s) 273
BseGI GGATG 1 cut(s) 472
BseLI CCNNNNNNNGG 1 cut(s) 274
BseMII CTCAG 1 cut(s) 605
BseNI ACTGG 3 cut(s) 620, 779, 789
BseRI GAGGAG 1 cut(s) 302
BseXI GCAGC 3 cut(s) 659, 677, 741
BsgI GTGCAG 1 cut(s) 257
Bsh1285I CGRYCG 1 cut(s) 261
BshNI GGYRCC 1 cut(s) 390
BshTI ACCGGT 1 cut(s) 747
BshVI ATCGAT 1 cut(s) 501
BsiEI CGRYCG 1 cut(s) 261
BsiSI CCGG 3 cut(s) 56, 72, 748
BslFI GGGAC 2 cut(s) 381, 419
BslI CCNNNNNNNGG 1 cut(s) 274
BsmAI GTCTC 1 cut(s) 168
BsmFI GGGAC 2 cut(s) 381, 419
BsmI GAATGC 1 cut(s) 604
Bsp119I TTCGAA 1 cut(s) 155
BspACI CCGC 2 cut(s) 46, 69
BspCNI CTCAG 1 cut(s) 606
BspDI ATCGAT 1 cut(s) 501
BspLI GGNNCC 1 cut(s) 392
BspMI ACCTGC 1 cut(s) 723
BspT104I TTCGAA 1 cut(s) 155
BspT107I GGYRCC 1 cut(s) 390
BsrFI RCCGGY 3 cut(s) 55, 71, 747
BsrI ACTGG 3 cut(s) 620, 779, 789
BssAI RCCGGY 3 cut(s) 55, 71, 747
BssECI CCNNGG 1 cut(s) 273
BssT1I CCWWGG 1 cut(s) 273
Bst4CI ACNGT 3 cut(s) 269, 372, 639
Bst6I CTCTTC 2 cut(s) 414, 483
BstBI TTCGAA 1 cut(s) 155
BstDEI CTNAG 2 cut(s) 33, 614
BstEII GGTNACC 1 cut(s) 77
BstF5I GGATG 1 cut(s) 472
BstMAI GTCTC 1 cut(s) 168
BstMCI CGRYCG 1 cut(s) 261
BstMWI GCNNNNNNNGC 3 cut(s) 65, 653, 662
BstNSI RCATGY 1 cut(s) 602
BstPI GGTNACC 1 cut(s) 77
BstSFI CTRYAG 1 cut(s) 385
BstV1I GCAGC 3 cut(s) 659, 677, 741
BstV2I GAAGAC 1 cut(s) 300
Bsu15I ATCGAT 1 cut(s) 501
BsuI GTATCC 1 cut(s) 585
BsuTUI ATCGAT 1 cut(s) 501
BtrI CACGTC 1 cut(s) 89
BtsCI GGATG 1 cut(s) 472
BtsIMutI CAGTG 1 cut(s) 644
BveI ACCTGC 1 cut(s) 723
Cfr10I RCCGGY 3 cut(s) 55, 71, 747
Cfr13I GGNCC 1 cut(s) 750
ClaI ATCGAT 1 cut(s) 501
Csp6I GTAC 4 cut(s) 101, 391, 401, 611
CspAI ACCGGT 1 cut(s) 747
CviAII CATG 2 cut(s) 599, 785
CviJI RGCY 8 cut(s) 59, 68, 222, 250, 282, 321, 411, 519
CviKI_1 RGCY 8 cut(s) 59, 68, 222, 250, 282, 321, 411, 519
CviQI GTAC 4 cut(s) 101, 391, 401, 611
DdeI CTNAG 2 cut(s) 33, 614
Eam1104I CTCTTC 2 cut(s) 414, 483
EarI CTCTTC 2 cut(s) 414, 483
Eco130I CCWWGG 1 cut(s) 273
Eco32I GATATC 1 cut(s) 499
Eco47I GGWCC 1 cut(s) 750
Eco57I CTGAAG 1 cut(s) 315
Eco91I GGTNACC 1 cut(s) 77
EcoO65I GGTNACC 1 cut(s) 77
EcoRV GATATC 1 cut(s) 499
EcoT14I CCWWGG 1 cut(s) 273
ErhI CCWWGG 1 cut(s) 273
FaeI CATG 2 cut(s) 602, 788
FaqI GGGAC 2 cut(s) 381, 419
FatI CATG 2 cut(s) 598, 784
Fnu4HI GCNGC 5 cut(s) 47, 69, 648, 666, 730
FokI GGATG 1 cut(s) 479
Fsp4HI GCNGC 5 cut(s) 47, 69, 648, 666, 730
FspBI CTAG 2 cut(s) 555, 585
GluI GCNGC 5 cut(s) 47, 69, 648, 666, 730
HapII CCGG 3 cut(s) 56, 72, 748
Hin1II CATG 2 cut(s) 602, 788
HincII GTYRAC 1 cut(s) 745
HindII GTYRAC 1 cut(s) 745
HinfI GANTC 6 cut(s) 110, 347, 431, 566, 616, 628
HpaI GTTAAC 1 cut(s) 745
HpaII CCGG 3 cut(s) 56, 72, 748
HphI GGTGA 1 cut(s) 89
Hpy166II GTNNAC 2 cut(s) 745, 753
Hpy188I TCNGA 5 cut(s) 163, 231, 436, 535, 571
Hpy188III TCNNGA 1 cut(s) 555
Hpy8I GTNNAC 2 cut(s) 745, 753
HpyAV CCTTC 2 cut(s) 419, 518
HpyCH4III ACNGT 3 cut(s) 269, 372, 639
HpyCH4IV ACGT 2 cut(s) 88, 453
HpyCH4V TGCA 6 cut(s) 143, 171, 238, 604, 698, 769
HpyF10VI GCNNNNNNNGC 3 cut(s) 65, 653, 662
HpyF3I CTNAG 2 cut(s) 33, 614
HpySE526I ACGT 2 cut(s) 88, 453
Hsp92II CATG 2 cut(s) 602, 788
KpnI GGTACC 1 cut(s) 394
KspAI GTTAAC 1 cut(s) 745
LmnI GCTCC 1 cut(s) 187
Lsp1109I GCAGC 3 cut(s) 659, 677, 741
LweI GCATC 2 cut(s) 344, 708
MaeI CTAG 2 cut(s) 555, 585
MaeII ACGT 2 cut(s) 88, 453
MaeIII GTNAC 2 cut(s) 77, 118
MboII GAAGA 6 cut(s) 49, 305, 314, 428, 431, 500
MluCI AATT 3 cut(s) 123, 808, 823
MlyI GAGTC 1 cut(s) 625
MnlI CCTC 8 cut(s) 280, 283, 388, 415, 457, 529, 562, 771
MseI TTAA 2 cut(s) 744, 806
MslI CAYNNNNRTG 1 cut(s) 87
MspI CCGG 3 cut(s) 56, 72, 748
Mva1269I GAATGC 1 cut(s) 604
MwoI GCNNNNNNNGC 3 cut(s) 65, 653, 662
NlaIII CATG 2 cut(s) 602, 788
NlaIV GGNNCC 1 cut(s) 392
NmeAIII GCCGAG 1 cut(s) 545
NmuCI GTSAC 2 cut(s) 77, 118
NspI RCATGY 1 cut(s) 602
NspV TTCGAA 1 cut(s) 155
PcsI WCGNNNNNNNCGW 1 cut(s) 517
PctI GAATGC 1 cut(s) 604
PfeI GAWTC 5 cut(s) 110, 347, 431, 566, 628
PinAI ACCGGT 1 cut(s) 747
PkrI GCNGC 5 cut(s) 48, 70, 649, 667, 731
PleI GAGTC 1 cut(s) 624
PpsI GAGTC 1 cut(s) 624
PsiI TTATAA 1 cut(s) 594
PspEI GGTNACC 1 cut(s) 77
PspN4I GGNNCC 1 cut(s) 392
PspPI GGNCC 1 cut(s) 750
RsaI GTAC 4 cut(s) 102, 392, 402, 612
RsaNI GTAC 4 cut(s) 101, 391, 401, 611
RseI CAYNNNNRTG 1 cut(s) 87
SaqAI TTAA 2 cut(s) 744, 806
SatI GCNGC 5 cut(s) 47, 69, 648, 666, 730
Sau96I GGNCC 1 cut(s) 750
SchI GAGTC 1 cut(s) 625
SfaNI GCATC 2 cut(s) 344, 708
SfcI CTRYAG 1 cut(s) 385
SfuI TTCGAA 1 cut(s) 155
SgrAI CRCCGGYG 1 cut(s) 71
SinI GGWCC 1 cut(s) 750
SmiMI CAYNNNNRTG 1 cut(s) 87
Sse9I AATT 3 cut(s) 123, 808, 823
SsiI CCGC 2 cut(s) 46, 69
SspI AATATT 2 cut(s) 694, 702
SspMI CTAG 2 cut(s) 555, 585
StyI CCWWGG 1 cut(s) 273
TaaI ACNGT 3 cut(s) 269, 372, 639
TaiI ACGT 2 cut(s) 91, 456
TaqI TCGA 6 cut(s) 108, 155, 257, 501, 737, 797
TaqII GACCGA 1 cut(s) 275
TasI AATT 3 cut(s) 123, 808, 823
TatI WGTACW 1 cut(s) 610
TauI GCSGC 2 cut(s) 49, 71
TfiI GAWTC 5 cut(s) 110, 347, 431, 566, 628
Tru1I TTAA 2 cut(s) 744, 806
Tru9I TTAA 2 cut(s) 744, 806
TscAI CASTG 1 cut(s) 644
TseFI GTSAC 2 cut(s) 77, 118
TseI GCWGC 3 cut(s) 647, 665, 729
Tsp45I GTSAC 2 cut(s) 77, 118
TspDTI ATGAA 4 cut(s) 102, 501, 519, 773
TspRI CASTG 1 cut(s) 644
VpaK11BI GGWCC 1 cut(s) 750
XapI RAATTY 1 cut(s) 808
XbaI TCTAGA 1 cut(s) 554
XceI RCATGY 1 cut(s) 602
XspI CTAG 2 cut(s) 555, 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.