Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0010119

Physical Location & Seq

Genomic Coordinates Forward (+)

23485825 .. 23489519

Gene Structure 3695 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1229900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2877 bp

ATGGAAAGGAACTCGGCAGCATGTGTGATGCAATGGAGCATCGAGCTAGAGAAAGCCCTGCGCTCCAAGAAACCAGGTCTCTCTCTCGAAACCATTTCGGAAGTCGGTGCAAGGCTCCAACAGTTAAGCACGGAGCCAGATGCTTCTCCTGCTGTCCACCACCTGTTCGACCTAATTCCTGGTGAGGAAAAGCTCTTCTCCAACACCATCATTCTTCGCCTTGCCAATGCCTTCCATTGTGGAGACAAGAATATAAGGCTCTCTATTGTCAGGGCCTTTCTGTTTTATGGGGATGTTGAGGATAGGGCATTGGCTTTGGGTCTGTTTGGTTGTTGGGCACATTTTGCTAAAGAGAGTTCGAGCATACGCTACTTGGTGCTTTCTAGTTTGGTTTCTTCTCACGTTATGGAGGTAAAAGCATCGCTGTTTGCAGCAGGATGTTTTGCCGAGTTATCAAAGGACTTTGCATGTGTTGTCTTGGAGATGCTGCCTCATATGATGACTTCACCAGAAACGTCACCTGCTATAAGGTTGGCTGGAGCACGAGTATTTGCCAAACTTCGGTGCTCACATCCCCTTGCGAATAATGCTTACAAGACATGCCTAAAGCTTCTGTTAGAGTCCGCGGATGAGGGTTGCCAGGTTGCAATGCTGGTTTCTCTCTCCAAACTGGCTTCCAGGTCAACCATTCTTATATCTCAGCAGATGGATTTGCTTCTCTTGTTTCTTAGCCAAGAAAAACCTCTTCAGCTGCGTGCAACGGCAGTAAGAAGCCTGCATTATATATACAGTCAAGGAATGTGTCATTTTCCTGTAAATGCATATCTTGTGAGAGCACTATTTAACATACTAGATAAACCTCAAGTTCCGTCACTCATACTATGTGATGCACTGCAGACATTACGTAAGATTATTTCATGTAAGCTAGCAGGTCTACCCTTCGATGTATTTGAATCTTCTAAGCTGTTAACCGTTGCTGAGAATGCAACTTCATCTCCAATCACGTCTGAGGGCCTGTTAGCTATTTCTGTTTTGGTAGATGTGCCAACGAAACTAAAAGGAAGTACAGAGATGGGGTCTCTTGCACCTTGTTCCTGTCTTCTGCCATCTCGTGTGATCTCGCTTATCATTGATCGAATCACCTTGATGGTTAAGCCAGTTTTGGATCTTTGTCGGACTGAGTCTCCAGTGTTTCAACAAATTAATTGCCTGCTCAATCTTCTTTTCTGTCTAATTCGAGAATATCCGGATATGCATGTCTTAGTGCTGGATCAAATATATGTTTTGGTCAAATTTTTGGTGGCCTTTCTTGAAAATCTCATTGAAGCTGGCGCCATCTCCACTGAAGTAGTTGACAAAGTGAAGCTTCTGGTCGAACTTGTAAGTCAGAGCAACTTGTTTGGATGTTATACATACACGCTCTACTCTTTGTTGTTACATTGTCGCATCATTTGGGTTAACTTGGCGCATGGGAGTGAGGGAAGTTGCAATCTTGATAAGAACTCGGGTGTATCTCACTTAATGGAGCATGAAATTCGTACAATTGAATGTGCGAACAGGATGCTGACAGAAAACAACAAGTGGCCTGCTTACAGAGTCGGGATTTATGCAGCATGTCAGGGAGCCTGGCTCACTGCCACTTTCACATTCGAGCAGTTAGTACCAAAGGTTCATTCTATTTCCTGCTGCTGCTGGATGAGATCATTAGTTCAATTTTCTGATTCTGAGAGGAAACTCAAGTCTCTGCTGTTGGCAAAACAAGGTTTAGAGACGCATAAATTCGATCTTATACATTTTAGTAATGATTCAGGTGAAATCAGCCAAGATTCAGCTAGTAATATCAGGGAGCATATTAACAGTAGGGAGCTAAAAGCGGCTTACGATGGTCTTTGTTCTTCTTTGGTAACATTAAAAGGTGATTTCAAAATGGACCAAACTTTTTATTTCCAAAGGTGGTTTTTGTCTTTAAGAGCAAAGGTGCTAGGAGCTGTGGTGGATATAGTTAAAGTTCTTGAAAACATTCAATTTGACCAGCACAGCTTTACCAATAATGAACAGGTTGGATACCCTGTATCCTTGAAGAAGTGTACTCAAATATCTGTGCAGTTGAAGAGGCTATCACAAGAATTTGATCTAGTAACAAAATCTTTCATTGACATGGACAGAAAAAGTTCAGGAATCATTTCGGAACTCGCACTATCTTGTTCACTGTTGGCCTTTTGTACTGGTTTTTCTCTTTACATTCCAAGCCTAATCGAATCTGTTTCTATTTTCGGTCTGGGAACAACAGAGAACAACTTAGACTCAACGTTGATACAAAATCTAATTGGGCGTTTGGGGGCCAGTAACCATGAAACCAGTGCAAATCTTTGTCTGCTTTTGAATCCGGGTGGCAAGCCCATTGATTGTTTTCACTTGCAGTCCAGAATCCAAGCTTGTAAGGTTGGTTCTGTAGTGAAAGATATTCTCAGTGTTTGTAGTTCTGTGGTTTTGGGGATTTTTGGCTTGAAAAGTAAGGCAAACGGAGTGCACAACGTGGAAGGTCTCTCCCAAGCAACCAAGGATGGCTTACAACTTCTGTATAGCATCCTAATGAAATGGATGCAAATTCCCTTCCAGACTCCCAAGTACTTTAAATTTAGGCCTTGCCTGGGGTCAGAACTCTTCGCTGTCAGTGAAACGAGAAACCCAGATGGAATATATGTATCCTCAGGCTTCAACTTATCATTAAATTTGTTCCTTCAGTTGAGAAATATGCCCCCAGACATCCGAGTCCGGTTGAAAAATTTGTACTGCATGGTCTACAGTGGAGCATCCTTTCAGGAGCCAAGAGAAAGTGGAGAAAACAAGGAGCAGTTACAGTATGTAAAAGGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

959

Amino Acids

106.47

Weight (kDa)

7.94

Isoelectric Point (pI)

45.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
INTS7_N	PF24436	102 - 442	5.1e-32	Integrator complex subunit 7, N-terminal
INTS7_C_plants	PF22966	885 - 952	1.6e-16	Integrator complex subunit 7, C-terminal domain in plants

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010119)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G20060
fragaria_vesca	FvH4_4g05080 FvH4_4g05080 FvH4_4g05080 FvH4_4g05080
malus_domestica	MD13G1224700.v1.1 MD16G1229900.v1.1
prunus_persica	Prupe.3G104300_v2.0.a1
pyrus_communis	pycom13g19830 pycom16g19170
rosa_chinensis	RchiOBHm_Chr4g0396011
rosa_laevigata	RLG00000009581
rosa_multiflora	Rmu_sc0012989.1_g000013 Rmu_sc0015594.1_g000011
rosa_roxburghii	Rroxscaffold_5G00340800
rosa_rugosa	Rorug03G0352900
rosa_samantha	Rh4BG063300 Rh4CG069900 Rh4CG070000 Rh4DG059900
rosa_wichuraiana	Rw4G005230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	529
AasI	GACNNNNNNGTC	1 cut(s)	2771
Acc36I	ACCTGC	2 cut(s)	529, 920
AccB1I	GGYRCC	1 cut(s)	1331
AccI	GTMKAC	2 cut(s)	934, 2802
AccII	CGCG	1 cut(s)	626
AccIII	TCCGGA	1 cut(s)	1246
AciI	CCGC	3 cut(s)	624, 626, 1874
AclI	AACGTT	1 cut(s)	2309
AclWI	GGATC	2 cut(s)	1173, 1278
AcsI	RAATTY	8 cut(s)	1292, 1533, 1778, 2126, 2607, 2636, 2731, 2785
AcuI	CTGAAG	3 cut(s)	731, 1365, 2726
AcyI	GRCGYC	1 cut(s)	1332
AdeI	CACNNNGTG	1 cut(s)	2536
AfaI	GTAC	7 cut(s)	1066, 1540, 1662, 2089, 2224, 2630, 2792
AfiI	CCNNNNNNNGG	2 cut(s)	561, 2651
AjiI	CACGTC	1 cut(s)	1005
AjnI	CCWGG	6 cut(s)	73, 178, 639, 677, 1625, 2649
AjuI	GAANNNNNNNTTGG	2 cut(s)	1657, 1689
Alw21I	GWGCWC	4 cut(s)	544, 569, 838, 2532
Alw26I	GTCTC	7 cut(s)	83, 237, 1083, 1188, 1746, 1763, 2549
Alw44I	GTGCAC	1 cut(s)	2528
AlwI	GGATC	2 cut(s)	1173, 1278
Ama87I	CYCGRG	1 cut(s)	1504
Aor13HI	TCCGGA	1 cut(s)	1246
AoxI	GGCC	7 cut(s)	273, 1012, 1302, 1583, 2214, 2340, 2642
ApaLI	GTGCAC	1 cut(s)	2528
ApeKI	GCWGC	7 cut(s)	17, 431, 487, 751, 1610, 1686, 1689
ApoI	RAATTY	8 cut(s)	1292, 1533, 1778, 2126, 2607, 2636, 2731, 2785
AseI	ATTAAT	1 cut(s)	1203
Asp700I	GAANNNNTTC	3 cut(s)	2019, 2170, 2182
AspLEI	GCGC	3 cut(s)	63, 1334, 1468
AspS9I	GGNCC	4 cut(s)	273, 1012, 1930, 2340
AsuC2I	CCSGG	1 cut(s)	2388
AsuHPI	GGTGA	6 cut(s)	194, 498, 510, 1132, 1823, 1928
AsuNHI	GCTAGC	1 cut(s)	925
AvaI	CYCGRG	1 cut(s)	1504
AvaII	GGWCC	1 cut(s)	1930
AxyI	CCTNAGG	1 cut(s)	2710
BaeGI	GKGCMC	2 cut(s)	340, 2532
BanI	GGYRCC	1 cut(s)	1331
BauI	CACGAG	2 cut(s)	543, 1110
BbsI	GAAGAC	1 cut(s)	1091
Bbv12I	GWGCWC	4 cut(s)	544, 569, 838, 2532
BbvI	GCAGC	7 cut(s)	29, 443, 474, 738, 1622, 1673, 1676
BccI	CCATC	9 cut(s)	215, 700, 1066, 1114, 1141, 1343, 1877, 2558, 2687
BceAI	ACGGC	1 cut(s)	777
BcgI	CGANNNNNNTGC	4 cut(s)	1517, 1543, 1551, 1577
BciT130I	CCWGG	6 cut(s)	75, 180, 641, 679, 1627, 2651
BciVI	GTATCC	3 cut(s)	2057, 2083, 2716
BcnI	CCSGG	1 cut(s)	2388
BcoDI	GTCTC	7 cut(s)	83, 237, 1083, 1188, 1746, 1763, 2549
BfaI	CTAG	7 cut(s)	47, 384, 851, 926, 1833, 1982, 2135
BfmI	CTRYAG	3 cut(s)	893, 2451, 2803
BfoI	RGCGCY	1 cut(s)	1335
BfuAI	ACCTGC	2 cut(s)	529, 920
BfuI	GTATCC	3 cut(s)	2057, 2083, 2716
BisI	GCNGC	8 cut(s)	18, 432, 488, 752, 1611, 1687, 1690, 1875
BlsI	GCNGC	8 cut(s)	19, 433, 489, 753, 1612, 1688, 1691, 1876
BmcAI	AGTACT	1 cut(s)	2630
Bme1390I	CCNGG	7 cut(s)	75, 180, 641, 679, 1627, 2388, 2651
Bme18I	GGWCC	1 cut(s)	1930
BmeT110I	CYCGRG	1 cut(s)	1504
BmgBI	CACGTC	1 cut(s)	1005
BmgT120I	GGNCC	4 cut(s)	273, 1012, 1930, 2340
BmiI	GGNNCC	6 cut(s)	116, 135, 1333, 1624, 2341, 2826
BmrFI	CCNGG	7 cut(s)	75, 180, 641, 679, 1627, 2388, 2651
BmtI	GCTAGC	1 cut(s)	929
BpiI	GAAGAC	1 cut(s)	1091
BplI	GAGNNNNNCTC	2 cut(s)	1614, 1646
BpmI	CTGGAG	2 cut(s)	558, 1170
BpuEI	CTTGAG	2 cut(s)	846, 1721
BpuMI	CCSGG	1 cut(s)	2388
BsaAI	YACGTR	1 cut(s)	905
BsaHI	GRCGYC	1 cut(s)	1332
BsaI	GGTCTC	3 cut(s)	83, 1083, 2549
BsaJI	CCNNGG	3 cut(s)	624, 2559, 2650
BsaWI	WCCGGW	2 cut(s)	1246, 2775
BsaXI	ACNNNNNCTCC	8 cut(s)	531, 561, 979, 1009, 1168, 1198, 1977, 2007
Bsc4I	CCNNNNNNNGG	2 cut(s)	561, 2651
Bse1I	ACTGG	6 cut(s)	675, 1157, 1187, 2230, 2343, 2358
Bse21I	CCTNAGG	1 cut(s)	2710
Bse3DI	GCAATG	2 cut(s)	38, 654
BseAI	TCCGGA	1 cut(s)	1246
BseBI	CCWGG	6 cut(s)	75, 180, 641, 679, 1627, 2651
BseDI	CCNNGG	3 cut(s)	624, 2559, 2650
BseLI	CCNNNNNNNGG	2 cut(s)	561, 2651
BseMI	GCAATG	2 cut(s)	38, 654
BseMII	CTCAG	7 cut(s)	713, 969, 999, 1170, 1716, 2482, 2724
BseNI	ACTGG	6 cut(s)	675, 1157, 1187, 2230, 2343, 2358
BseSI	GKGCMC	2 cut(s)	340, 2532
BseXI	GCAGC	7 cut(s)	29, 443, 474, 738, 1622, 1673, 1676
BsgI	GTGCAG	1 cut(s)	2123
Bsh1236I	CGCG	1 cut(s)	626
BshFI	GGCC	7 cut(s)	275, 1014, 1304, 1585, 2216, 2342, 2644
BshNI	GGYRCC	1 cut(s)	1331
BsiHKAI	GWGCWC	4 cut(s)	544, 569, 838, 2532
BsiHKCI	CYCGRG	1 cut(s)	1504
BsiSI	CCGG	3 cut(s)	1247, 2387, 2776
BslI	CCNNNNNNNGG	2 cut(s)	561, 2651
BsmAI	GTCTC	7 cut(s)	83, 237, 1083, 1188, 1746, 1763, 2549
BsmBI	CGTCTC	1 cut(s)	1763
BsmI	GAATGC	1 cut(s)	988
BsnI	GGCC	7 cut(s)	275, 1014, 1304, 1585, 2216, 2342, 2644
Bso31I	GGTCTC	3 cut(s)	83, 1083, 2549
BsoBI	CYCGRG	1 cut(s)	1504
Bsp1286I	GDGCHC	5 cut(s)	340, 544, 569, 838, 2532
Bsp13I	TCCGGA	1 cut(s)	1246
Bsp143I	GATC	7 cut(s)	1116, 1132, 1165, 1270, 1700, 1783, 2131
BspACI	CCGC	3 cut(s)	624, 626, 1874
BspANI	GGCC	7 cut(s)	275, 1014, 1304, 1585, 2216, 2342, 2644
BspCNI	CTCAG	7 cut(s)	712, 970, 1000, 1171, 1717, 2481, 2723
BspEI	TCCGGA	1 cut(s)	1246
BspFNI	CGCG	1 cut(s)	626
BspLI	GGNNCC	6 cut(s)	116, 135, 1333, 1624, 2341, 2826
BspMAI	CTGCAG	1 cut(s)	897
BspMI	ACCTGC	2 cut(s)	529, 920
BspOI	GCTAGC	1 cut(s)	929
BspPI	GGATC	2 cut(s)	1173, 1278
BspQI	GCTCTTC	1 cut(s)	200
BspT107I	GGYRCC	1 cut(s)	1331
BspTNI	GGTCTC	3 cut(s)	83, 1083, 2549
BsrDI	GCAATG	2 cut(s)	38, 654
BsrI	ACTGG	6 cut(s)	675, 1157, 1187, 2230, 2343, 2358
BssECI	CCNNGG	3 cut(s)	624, 2559, 2650
BssMI	GATC	7 cut(s)	1116, 1132, 1165, 1270, 1700, 1783, 2131
BssNI	GRCGYC	1 cut(s)	1332
BssSI	CACGAG	2 cut(s)	543, 1110
BssT1I	CCWWGG	1 cut(s)	2559
Bst2BI	CACGAG	2 cut(s)	543, 1110
Bst2UI	CCWGG	6 cut(s)	75, 180, 641, 679, 1627, 2651
Bst4CI	ACNGT	7 cut(s)	123, 791, 973, 1859, 2211, 2807, 2862
Bst6I	CTCTTC	4 cut(s)	200, 750, 2105, 2669
BstACI	GRCGYC	1 cut(s)	1332
BstAPI	GCANNNNNTGC	1 cut(s)	344
BstBAI	YACGTR	1 cut(s)	905
BstC8I	GCNNGC	7 cut(s)	756, 776, 927, 1211, 1330, 1587, 2396
BstDSI	CCRYGG	1 cut(s)	624
BstFNI	CGCG	1 cut(s)	626
BstH2I	RGCGCY	1 cut(s)	1335
BstHHI	GCGC	3 cut(s)	63, 1334, 1468
BstKTI	GATC	7 cut(s)	1119, 1135, 1168, 1273, 1703, 1786, 2134
BstMAI	GTCTC	7 cut(s)	83, 237, 1083, 1188, 1746, 1763, 2549
BstMBI	GATC	7 cut(s)	1116, 1132, 1165, 1270, 1700, 1783, 2131
BstMWI	GCNNNNNNNGC	4 cut(s)	149, 344, 587, 983
BstNI	CCWGG	6 cut(s)	75, 180, 641, 679, 1627, 2651
BstNSI	RCATGY	5 cut(s)	24, 471, 603, 1259, 1617
BstSCI	CCNGG	7 cut(s)	73, 178, 639, 677, 1625, 2386, 2649
BstSFI	CTRYAG	3 cut(s)	893, 2451, 2803
BstSLI	GKGCMC	2 cut(s)	340, 2532
BstSNI	TACGTA	1 cut(s)	905
BstUI	CGCG	1 cut(s)	626
BstV1I	GCAGC	7 cut(s)	29, 443, 474, 738, 1622, 1673, 1676
BstV2I	GAAGAC	1 cut(s)	1091
BstX2I	RGATCY	1 cut(s)	1165
BstYI	RGATCY	1 cut(s)	1165
Bsu36I	CCTNAGG	1 cut(s)	2710
BsuI	GTATCC	3 cut(s)	2057, 2083, 2716
BsuRI	GGCC	7 cut(s)	275, 1014, 1304, 1585, 2216, 2342, 2644
BtgI	CCRYGG	1 cut(s)	624
BtgZI	GCGATG	1 cut(s)	405
BtrI	CACGTC	1 cut(s)	1005
BtsI	GCAGTG	2 cut(s)	890, 1632
BtsIMutI	CAGTG	9 cut(s)	890, 1194, 1341, 1632, 2207, 2365, 2476, 2680, 2812
BveI	ACCTGC	2 cut(s)	529, 920
Cac8I	GCNNGC	7 cut(s)	756, 776, 927, 1211, 1330, 1587, 2396
CfoI	GCGC	3 cut(s)	63, 1334, 1468
Cfr13I	GGNCC	4 cut(s)	273, 1012, 1930, 2340
Cfr42I	CCGCGG	1 cut(s)	627
CseI	GACGC	1 cut(s)	1780
CsiI	ACCWGGT	1 cut(s)	73
Csp6I	GTAC	7 cut(s)	1065, 1539, 1661, 2088, 2223, 2629, 2791
CviQI	GTAC	7 cut(s)	1065, 1539, 1661, 2088, 2223, 2629, 2791
DinI	GGCGCC	1 cut(s)	1333
DpnI	GATC	7 cut(s)	1118, 1134, 1167, 1272, 1702, 1785, 2133
DpnII	GATC	7 cut(s)	1116, 1132, 1165, 1270, 1700, 1783, 2131
DraI	TTTAAA	1 cut(s)	2635
DraIII	CACNNNGTG	1 cut(s)	2536
DrdI	GACNNNNNNGTC	1 cut(s)	2771
DseDI	GACNNNNNNGTC	1 cut(s)	2771
Eam1104I	CTCTTC	4 cut(s)	200, 750, 2105, 2669
EarI	CTCTTC	4 cut(s)	200, 750, 2105, 2669
Eco105I	TACGTA	1 cut(s)	905
Eco130I	CCWWGG	1 cut(s)	2559
Eco147I	AGGCCT	1 cut(s)	2644
Eco31I	GGTCTC	3 cut(s)	83, 1083, 2549
Eco47I	GGWCC	1 cut(s)	1930
Eco57I	CTGAAG	3 cut(s)	731, 1365, 2726
Eco81I	CCTNAGG	1 cut(s)	2710
Eco88I	CYCGRG	1 cut(s)	1504
EcoO109I	RGGNCCY	2 cut(s)	273, 1012
EcoRII	CCWGG	6 cut(s)	73, 178, 639, 677, 1625, 2649
EcoT14I	CCWWGG	1 cut(s)	2559
EcoT22I	ATGCAT	2 cut(s)	823, 1257
EgeI	GGCGCC	1 cut(s)	1333
EheI	GGCGCC	1 cut(s)	1333
ErhI	CCWWGG	1 cut(s)	2559
Esp3I	CGTCTC	1 cut(s)	1763
FalI	AAGNNNNNCTT	4 cut(s)	1350, 1382, 2552, 2584
FauNDI	CATATG	1 cut(s)	495
FblI	GTMKAC	2 cut(s)	934, 2802
Fnu4HI	GCNGC	8 cut(s)	18, 432, 488, 752, 1611, 1687, 1690, 1875
Fsp4HI	GCNGC	8 cut(s)	18, 432, 488, 752, 1611, 1687, 1690, 1875
FspBI	CTAG	7 cut(s)	47, 384, 851, 926, 1833, 1982, 2135
GlaI	GCGC	3 cut(s)	62, 1333, 1467
GluI	GCNGC	8 cut(s)	18, 432, 488, 752, 1611, 1687, 1690, 1875
GsuI	CTGGAG	2 cut(s)	558, 1170
HaeII	RGCGCY	1 cut(s)	1335
HaeIII	GGCC	7 cut(s)	275, 1014, 1304, 1585, 2216, 2342, 2644
HapII	CCGG	3 cut(s)	1247, 2387, 2776
HgaI	GACGC	1 cut(s)	1780
HhaI	GCGC	3 cut(s)	63, 1334, 1468
Hin1I	GRCGYC	1 cut(s)	1332
Hin6I	GCGC	3 cut(s)	61, 1332, 1466
HinP1I	GCGC	3 cut(s)	61, 1332, 1466
HincII	GTYRAC	4 cut(s)	684, 969, 1354, 1459
HindII	GTYRAC	4 cut(s)	684, 969, 1354, 1459
HindIII	AAGCTT	3 cut(s)	608, 1364, 2433
HpaI	GTTAAC	2 cut(s)	969, 1459
HpaII	CCGG	3 cut(s)	1247, 2387, 2776
HphI	GGTGA	6 cut(s)	194, 498, 510, 1132, 1823, 1928
Hpy188I	TCNGA	9 cut(s)	100, 1009, 1176, 1389, 1720, 1726, 2188, 2659, 2771
HpyAV	CCTTC	5 cut(s)	241, 949, 2534, 2623, 2750
HpyCH4III	ACNGT	7 cut(s)	123, 791, 973, 1859, 2211, 2807, 2862
HpyCH4IV	ACGT	6 cut(s)	402, 515, 904, 1004, 2309, 2535
HpyF10VI	GCNNNNNNNGC	4 cut(s)	149, 344, 587, 983
HpySE526I	ACGT	6 cut(s)	402, 515, 904, 1004, 2309, 2535
Hsp92I	GRCGYC	1 cut(s)	1332
HspAI	GCGC	3 cut(s)	61, 1332, 1466
KasI	GGCGCC	1 cut(s)	1331
Kpn2I	TCCGGA	1 cut(s)	1246
KspAI	GTTAAC	2 cut(s)	969, 1459
KspI	CCGCGG	1 cut(s)	627
Kzo9I	GATC	7 cut(s)	1116, 1132, 1165, 1270, 1700, 1783, 2131
LguI	GCTCTTC	1 cut(s)	200
Lsp1109I	GCAGC	7 cut(s)	29, 443, 474, 738, 1622, 1673, 1676
MabI	ACCWGGT	1 cut(s)	73
MaeI	CTAG	7 cut(s)	47, 384, 851, 926, 1833, 1982, 2135
MaeII	ACGT	6 cut(s)	402, 515, 904, 1004, 2309, 2535
MaeIII	GTNAC	7 cut(s)	516, 870, 1434, 1903, 2137, 2345, 2856
MalI	GATC	7 cut(s)	1118, 1134, 1167, 1272, 1702, 1785, 2133
MboI	GATC	7 cut(s)	1116, 1132, 1165, 1270, 1700, 1783, 2131
MfeI	CAATTG	1 cut(s)	1542
MflI	RGATCY	1 cut(s)	1165
MhlI	GDGCHC	5 cut(s)	340, 544, 569, 838, 2532
Mly113I	GGCGCC	1 cut(s)	1332
MlyI	GAGTC	6 cut(s)	629, 1190, 1605, 2297, 2614, 2781
MmeI	TCCRAC	4 cut(s)	142, 225, 1154, 2041
Mph1103I	ATGCAT	2 cut(s)	823, 1257
MroI	TCCGGA	1 cut(s)	1246
MroXI	GAANNNNTTC	3 cut(s)	2019, 2170, 2182
MslI	CAYNNNNRTG	4 cut(s)	1145, 1473, 2156, 2591
MspA1I	CMGCKG	2 cut(s)	626, 751
MspI	CCGG	3 cut(s)	1247, 2387, 2776
MspR9I	CCNGG	7 cut(s)	75, 180, 641, 679, 1627, 2388, 2651
MunI	CAATTG	1 cut(s)	1542
Mva1269I	GAATGC	1 cut(s)	988
MvaI	CCWGG	6 cut(s)	75, 180, 641, 679, 1627, 2651
MvnI	CGCG	1 cut(s)	626
MwoI	GCNNNNNNNGC	4 cut(s)	149, 344, 587, 983
NarI	GGCGCC	1 cut(s)	1332
NciI	CCSGG	1 cut(s)	2388
NdeI	CATATG	1 cut(s)	495
NdeII	GATC	7 cut(s)	1116, 1132, 1165, 1270, 1700, 1783, 2131
NheI	GCTAGC	1 cut(s)	925
NlaIV	GGNNCC	6 cut(s)	116, 135, 1333, 1624, 2341, 2826
NmeAIII	GCCGAG	1 cut(s)	472
NmuCI	GTSAC	2 cut(s)	516, 870
NsiI	ATGCAT	2 cut(s)	823, 1257
NspI	RCATGY	5 cut(s)	24, 471, 603, 1259, 1617
PaqCI	CACCTGC	1 cut(s)	529
PceI	AGGCCT	1 cut(s)	2644
PciSI	GCTCTTC	1 cut(s)	200
PctI	GAATGC	1 cut(s)	988
PdmI	GAANNNNTTC	3 cut(s)	2019, 2170, 2182
PfeI	GAWTC	9 cut(s)	953, 1137, 1721, 1805, 1826, 2178, 2258, 2383, 2427
PflFI	GACNNNGTC	1 cut(s)	1180
PkrI	GCNGC	8 cut(s)	19, 433, 489, 753, 1612, 1688, 1691, 1876
PleI	GAGTC	6 cut(s)	628, 1189, 1604, 2297, 2614, 2780
PluTI	GGCGCC	1 cut(s)	1335
PpsI	GAGTC	6 cut(s)	628, 1189, 1604, 2297, 2614, 2780
Ppu21I	YACGTR	1 cut(s)	905
PshBI	ATTAAT	1 cut(s)	1203
Psp1406I	AACGTT	1 cut(s)	2309
Psp6I	CCWGG	6 cut(s)	73, 178, 639, 677, 1625, 2649
PspGI	CCWGG	6 cut(s)	73, 178, 639, 677, 1625, 2649
PspN4I	GGNNCC	6 cut(s)	116, 135, 1333, 1624, 2341, 2826
PspPI	GGNCC	4 cut(s)	273, 1012, 1930, 2340
PsrI	GAACNNNNNNTAC	4 cut(s)	1653, 1685, 2431, 2463
PstI	CTGCAG	1 cut(s)	897
PsuI	RGATCY	1 cut(s)	1165
PsyI	GACNNNGTC	1 cut(s)	1180
PvuII	CAGCTG	1 cut(s)	751
RsaI	GTAC	7 cut(s)	1066, 1540, 1662, 2089, 2224, 2630, 2792
RsaNI	GTAC	7 cut(s)	1065, 1539, 1661, 2088, 2223, 2629, 2791
RseI	CAYNNNNRTG	4 cut(s)	1145, 1473, 2156, 2591
SacII	CCGCGG	1 cut(s)	627
SapI	GCTCTTC	1 cut(s)	200
SatI	GCNGC	8 cut(s)	18, 432, 488, 752, 1611, 1687, 1690, 1875
Sau3AI	GATC	7 cut(s)	1116, 1132, 1165, 1270, 1700, 1783, 2131
Sau96I	GGNCC	4 cut(s)	273, 1012, 1930, 2340
ScaI	AGTACT	1 cut(s)	2630
SchI	GAGTC	6 cut(s)	629, 1190, 1605, 2297, 2614, 2781
ScrFI	CCNGG	7 cut(s)	75, 180, 641, 679, 1627, 2388, 2651
SduI	GDGCHC	5 cut(s)	340, 544, 569, 838, 2532
SexAI	ACCWGGT	1 cut(s)	73
SfcI	CTRYAG	3 cut(s)	893, 2451, 2803
SfoI	GGCGCC	1 cut(s)	1333
Sfr303I	CCGCGG	1 cut(s)	627
SgrBI	CCGCGG	1 cut(s)	627
SinI	GGWCC	1 cut(s)	1930
SmiMI	CAYNNNNRTG	4 cut(s)	1145, 1473, 2156, 2591
SmlI	CTYRAG	2 cut(s)	861, 1736
SmoI	CTYRAG	2 cut(s)	861, 1736
SnaBI	TACGTA	1 cut(s)	905
SseBI	AGGCCT	1 cut(s)	2644
SsiI	CCGC	3 cut(s)	624, 626, 1874
SspDI	GGCGCC	1 cut(s)	1331
SspMI	CTAG	7 cut(s)	47, 384, 851, 926, 1833, 1982, 2135
StuI	AGGCCT	1 cut(s)	2644
StyD4I	CCNGG	7 cut(s)	73, 178, 639, 677, 1625, 2386, 2649
StyI	CCWWGG	1 cut(s)	2559
TaaI	ACNGT	7 cut(s)	123, 791, 973, 1859, 2211, 2807, 2862
TaiI	ACGT	6 cut(s)	405, 518, 907, 1007, 2312, 2538
TaqII	GACCGA	1 cut(s)	2264
TatI	WGTACW	5 cut(s)	1064, 2087, 2222, 2628, 2790
TauI	GCSGC	1 cut(s)	1877
TfiI	GAWTC	9 cut(s)	953, 1137, 1721, 1805, 1826, 2178, 2258, 2383, 2427
TscAI	CASTG	9 cut(s)	897, 1194, 1348, 1639, 2214, 2365, 2476, 2680, 2812
TseFI	GTSAC	2 cut(s)	516, 870
TseI	GCWGC	7 cut(s)	17, 431, 487, 751, 1610, 1686, 1689
Tsp45I	GTSAC	2 cut(s)	516, 870
TspDTI	ATGAA	8 cut(s)	906, 981, 1545, 1661, 2067, 2140, 2367, 2609
TspGWI	ACGGA	3 cut(s)	146, 858, 2538
TspRI	CASTG	9 cut(s)	897, 1194, 1348, 1639, 2214, 2365, 2476, 2680, 2812
Tth111I	GACNNNGTC	1 cut(s)	1180
VneI	GTGCAC	1 cut(s)	2528
VpaK11BI	GGWCC	1 cut(s)	1930
VspI	ATTAAT	1 cut(s)	1203
XapI	RAATTY	8 cut(s)	1292, 1533, 1778, 2126, 2607, 2636, 2731, 2785
XceI	RCATGY	5 cut(s)	24, 471, 603, 1259, 1617
XmiI	GTMKAC	2 cut(s)	934, 2802
XmnI	GAANNNNTTC	3 cut(s)	2019, 2170, 2182
XspI	CTAG	7 cut(s)	47, 384, 851, 926, 1833, 1982, 2135
ZrmI	AGTACT	1 cut(s)	2630
Zsp2I	ATGCAT	2 cut(s)	823, 1257

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.