Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0017283

Physical Location & Seq

Genomic Coordinates Reverse (-)

26189432 .. 26191139

Gene Structure 1708 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1241900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1029 bp

ATGCTTCGAACAATAAGCTACTTCGATTTTAAGACATTGAAGAAGGCAACCAAGAACTTTCATCCTGGTAATCTCCTTGGAGTAGGTGGATTTGGCCCTGTCTATCGGGGGAGATTAGGAGATGGGACGTTAATTGCTGCTAAGAAATTGTGCCTTGACAAATCCCAACAAGGAGAATCAGAGTTTCTTACAGAGGTAAAGTTGATCACAAGCGTCCAACACAGGAACTTGGTTCGTCTTATCGGATGCTGCTCTGATGGGCCGCAACGGCTTCTTGTGTATGAATACATGAAGAACAGGAGCTTGGACCTCATTATTTATGGAAAGAGTGATCAGTTCTTGAGCTGGAGCACCAGGTTCCAGATAATTGTCGGTATTGCTCGAGGGTTACAATATCTACATGAGGATTCCCCCCTAAGAATTATCCACAGAGATATCAAGGCAAGCAACATTCTTCTTGACGACAAATTCCAACCGAAAATTGGAGATTTCGGGCTGGCTAGGTTCTTCCCTGAAGACCAAGCTTATCTCAGCACTACATTTGCTGGAACTTTAGGATATACGGCACCTGAGTATGCTATTAGAGGAGAATTATCTGAAAAGGCGGACATCTATAGTTTTGGAGTTCTTGTGCTCGAAATAATCAGCGGTAGAAAAAACACAGATCTCACTTTATCATCAGATATGCAATACCTCCCTGAATATGCATGGAAATTGTTTGAGACGTCGAATGTGATTGATCTGATAGATCCAAAAATAAAAGAAGATGGATATGTGGAAAGGGATGTCTTGCAAGCAACCCAAGTCGCCTTCTTCTGCCTTCAGCCGCACCCAAACCTTAGGCCCCCGATGTCGGAGGTTGTAGTAATGTTAACATGCGAAGTTGAAATGGTCGGAACACCTATGAAACCCGCCTTCTTGGGAAGAAGGCGAACGAAGAACCAGAACATTTCATGGGATTCTATAAGTGAGGCTTTCCCATCTCCCTTGCAGAGTGAGCCTCCCTCGTTAGCTAAACCATCCACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

343

Amino Acids

38.56

Weight (kDa)

7.61

Isoelectric Point (pI)

40.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	21 - 287	7.6e-45	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	24 - 288	5.8e-43	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0017283)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g06650
malus_domestica	MD13G1236800.v1.1 MD16G1241900.v1.1
prunus_persica	Prupe.1G068800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0398541
rosa_laevigata	RLG00000009346
rosa_roxburghii	Rroxscaffold_5G00343510
rosa_rugosa	Rorug04G0009200
rosa_samantha	Rh4AG087000 Rh4BG083700 Rh4CG095200 Rh4DG078600
rosa_wichuraiana	Rw4G007260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	728
AccB1I	GGYRCC	1 cut(s)	565
AciI	CCGC	5 cut(s)	263, 605, 648, 827, 912
AclWI	GGATC	1 cut(s)	743
AcsI	RAATTY	1 cut(s)	467
AcuI	CTGAAG	2 cut(s)	534, 806
AcyI	GRCGYC	1 cut(s)	725
AfiI	CCNNNNNNNGG	2 cut(s)	482, 853
AgsI	TTSAA	2 cut(s)	40, 887
AjnI	CCWGG	2 cut(s)	64, 353
AjuI	GAANNNNNNNTTGG	2 cut(s)	287, 319
AluBI	AGCT	5 cut(s)	18, 303, 345, 524, 1013
AluI	AGCT	5 cut(s)	18, 303, 345, 524, 1013
Alw21I	GWGCWC	2 cut(s)	353, 636
Alw26I	GTCTC	1 cut(s)	716
AlwI	GGATC	1 cut(s)	743
Ama87I	CYCGRG	1 cut(s)	381
AoxI	GGCC	3 cut(s)	94, 260, 842
ApeKI	GCWGC	2 cut(s)	137, 249
ApoI	RAATTY	1 cut(s)	467
AspS9I	GGNCC	4 cut(s)	95, 260, 307, 843
AsuII	TTCGAA	1 cut(s)	7
AvaI	CYCGRG	1 cut(s)	381
AvaII	GGWCC	1 cut(s)	307
AxyI	CCTNAGG	1 cut(s)	839
BanI	GGYRCC	1 cut(s)	565
BbsI	GAAGAC	1 cut(s)	522
Bbv12I	GWGCWC	2 cut(s)	353, 636
BbvI	GCAGC	2 cut(s)	124, 236
BccI	CCATC	5 cut(s)	116, 251, 761, 988, 1027
BceAI	ACGGC	2 cut(s)	284, 579
BciT130I	CCWGG	2 cut(s)	66, 355
BclI	TGATCA	2 cut(s)	204, 331
BcoDI	GTCTC	1 cut(s)	716
BfaI	CTAG	1 cut(s)	501
BfmI	CTRYAG	1 cut(s)	613
BglI	GCCNNNNNGGC	1 cut(s)	268
BglII	AGATCT	1 cut(s)	664
BisI	GCNGC	4 cut(s)	138, 250, 263, 827
BlsI	GCNGC	4 cut(s)	139, 251, 264, 828
Bme1390I	CCNGG	2 cut(s)	66, 355
Bme18I	GGWCC	1 cut(s)	307
BmeT110I	CYCGRG	1 cut(s)	381
BmgT120I	GGNCC	4 cut(s)	95, 260, 307, 843
BmiI	GGNNCC	3 cut(s)	359, 567, 845
BmrFI	CCNGG	2 cut(s)	66, 355
BmsI	GCATC	1 cut(s)	236
BpiI	GAAGAC	1 cut(s)	522
BplI	GAGNNNNNCTC	4 cut(s)	985, 1017, 989, 1021
BpmI	CTGGAG	1 cut(s)	367
Bpu14I	TTCGAA	1 cut(s)	7
BpuEI	CTTGAG	1 cut(s)	361
BsaHI	GRCGYC	1 cut(s)	725
BsaJI	CCNNGG	1 cut(s)	76
BsaXI	ACNNNNNCTCC	2 cut(s)	615, 645
Bsc4I	CCNNNNNNNGG	2 cut(s)	482, 853
Bse21I	CCTNAGG	1 cut(s)	839
BseBI	CCWGG	2 cut(s)	66, 355
BseDI	CCNNGG	1 cut(s)	76
BseGI	GGATG	4 cut(s)	61, 251, 790, 1019
BseLI	CCNNNNNNNGG	2 cut(s)	482, 853
BseMII	CTCAG	2 cut(s)	544, 561
BseRI	GAGGAG	1 cut(s)	600
BseXI	GCAGC	2 cut(s)	124, 236
BshFI	GGCC	3 cut(s)	96, 262, 844
BshNI	GGYRCC	1 cut(s)	565
BsiHKAI	GWGCWC	2 cut(s)	353, 636
BsiHKCI	CYCGRG	1 cut(s)	381
BslFI	GGGAC	1 cut(s)	139
BslI	CCNNNNNNNGG	2 cut(s)	482, 853
BsmAI	GTCTC	1 cut(s)	716
BsmBI	CGTCTC	1 cut(s)	716
BsmFI	GGGAC	1 cut(s)	139
BsnI	GGCC	3 cut(s)	96, 262, 844
BsoBI	CYCGRG	1 cut(s)	381
Bsp119I	TTCGAA	1 cut(s)	7
Bsp1286I	GDGCHC	2 cut(s)	353, 636
Bsp143I	GATC	5 cut(s)	204, 331, 664, 739, 748
BspACI	CCGC	5 cut(s)	263, 605, 648, 827, 912
BspANI	GGCC	3 cut(s)	96, 262, 844
BspCNI	CTCAG	2 cut(s)	543, 562
BspLI	GGNNCC	3 cut(s)	359, 567, 845
BspPI	GGATC	1 cut(s)	743
BspT104I	TTCGAA	1 cut(s)	7
BspT107I	GGYRCC	1 cut(s)	565
BssECI	CCNNGG	1 cut(s)	76
BssMI	GATC	5 cut(s)	204, 331, 664, 739, 748
BssNI	GRCGYC	1 cut(s)	725
BssT1I	CCWWGG	1 cut(s)	76
Bst2UI	CCWGG	2 cut(s)	66, 355
BstACI	GRCGYC	1 cut(s)	725
BstBI	TTCGAA	1 cut(s)	7
BstC8I	GCNNGC	3 cut(s)	445, 498, 795
BstDEI	CTNAG	5 cut(s)	141, 416, 530, 570, 839
BstF5I	GGATG	4 cut(s)	61, 251, 790, 1019
BstKTI	GATC	5 cut(s)	207, 334, 667, 742, 751
BstMAI	GTCTC	1 cut(s)	716
BstMBI	GATC	5 cut(s)	204, 331, 664, 739, 748
BstMWI	GCNNNNNNNGC	2 cut(s)	268, 997
BstNI	CCWGG	2 cut(s)	66, 355
BstNSI	RCATGY	1 cut(s)	879
BstSCI	CCNGG	2 cut(s)	64, 353
BstSFI	CTRYAG	1 cut(s)	613
BstV1I	GCAGC	2 cut(s)	124, 236
BstV2I	GAAGAC	1 cut(s)	522
BstX2I	RGATCY	2 cut(s)	664, 748
BstYI	RGATCY	2 cut(s)	664, 748
Bsu36I	CCTNAGG	1 cut(s)	839
BsuRI	GGCC	3 cut(s)	96, 262, 844
BtsCI	GGATG	4 cut(s)	61, 251, 790, 1019
Cac8I	GCNNGC	3 cut(s)	445, 498, 795
Cfr13I	GGNCC	4 cut(s)	95, 260, 307, 843
CseI	GACGC	1 cut(s)	202
CsiI	ACCWGGT	1 cut(s)	353
CviAII	CATG	5 cut(s)	289, 401, 708, 876, 954
DdeI	CTNAG	5 cut(s)	141, 416, 530, 570, 839
DpnI	GATC	5 cut(s)	206, 333, 666, 741, 750
DpnII	GATC	5 cut(s)	204, 331, 664, 739, 748
EciI	GGCGGA	1 cut(s)	620
Eco130I	CCWWGG	1 cut(s)	76
Eco32I	GATATC	1 cut(s)	436
Eco47I	GGWCC	1 cut(s)	307
Eco57I	CTGAAG	2 cut(s)	534, 806
Eco81I	CCTNAGG	1 cut(s)	839
Eco88I	CYCGRG	1 cut(s)	381
EcoO109I	RGGNCCY	1 cut(s)	843
EcoRII	CCWGG	2 cut(s)	64, 353
EcoRV	GATATC	1 cut(s)	436
EcoT14I	CCWWGG	1 cut(s)	76
EcoT22I	ATGCAT	1 cut(s)	709
ErhI	CCWWGG	1 cut(s)	76
Esp3I	CGTCTC	1 cut(s)	716
FaeI	CATG	5 cut(s)	292, 404, 711, 879, 957
FalI	AAGNNNNNCTT	2 cut(s)	958, 990
FaqI	GGGAC	1 cut(s)	139
FatI	CATG	5 cut(s)	288, 400, 707, 875, 953
FauI	CCCGC	1 cut(s)	919
FbaI	TGATCA	2 cut(s)	204, 331
Fnu4HI	GCNGC	4 cut(s)	138, 250, 263, 827
FokI	GGATG	4 cut(s)	48, 258, 797, 1006
Fsp4HI	GCNGC	4 cut(s)	138, 250, 263, 827
FspBI	CTAG	1 cut(s)	501
GluI	GCNGC	4 cut(s)	138, 250, 263, 827
GsuI	CTGGAG	1 cut(s)	367
HaeIII	GGCC	3 cut(s)	96, 262, 844
HgaI	GACGC	1 cut(s)	202
Hin1I	GRCGYC	1 cut(s)	725
Hin1II	CATG	5 cut(s)	292, 404, 711, 879, 957
HincII	GTYRAC	1 cut(s)	873
HindII	GTYRAC	1 cut(s)	873
HindIII	AAGCTT	1 cut(s)	522
HinfI	GANTC	3 cut(s)	176, 407, 959
HpaI	GTTAAC	1 cut(s)	873
Hpy166II	GTNNAC	1 cut(s)	873
Hpy188I	TCNGA	8 cut(s)	181, 245, 256, 598, 682, 744, 856, 896
Hpy188III	TCNNGA	3 cut(s)	340, 361, 458
Hpy8I	GTNNAC	1 cut(s)	873
Hpy99I	CGWCG	1 cut(s)	730
HpyAV	CCTTC	5 cut(s)	37, 820, 830, 921, 925
HpyCH4IV	ACGT	2 cut(s)	128, 725
HpyCH4V	TGCA	4 cut(s)	688, 707, 793, 991
HpyF10VI	GCNNNNNNNGC	2 cut(s)	268, 997
HpyF3I	CTNAG	5 cut(s)	141, 416, 530, 570, 839
HpySE526I	ACGT	2 cut(s)	128, 725
Hsp92I	GRCGYC	1 cut(s)	725
Hsp92II	CATG	5 cut(s)	292, 404, 711, 879, 957
Ksp22I	TGATCA	2 cut(s)	204, 331
KspAI	GTTAAC	1 cut(s)	873
Kzo9I	GATC	5 cut(s)	204, 331, 664, 739, 748
LmnI	GCTCC	2 cut(s)	300, 348
Lsp1109I	GCAGC	2 cut(s)	124, 236
LweI	GCATC	1 cut(s)	236
MabI	ACCWGGT	1 cut(s)	353
MaeI	CTAG	1 cut(s)	501
MaeII	ACGT	2 cut(s)	128, 725
MaeIII	GTNAC	1 cut(s)	387
MalI	GATC	5 cut(s)	206, 333, 666, 741, 750
MboI	GATC	5 cut(s)	204, 331, 664, 739, 748
MboII	GAAGA	9 cut(s)	52, 304, 446, 499, 527, 776, 805, 936, 949
MflI	RGATCY	2 cut(s)	664, 748
MhlI	GDGCHC	2 cut(s)	353, 636
MluCI	AATT	8 cut(s)	132, 146, 366, 420, 467, 480, 590, 713
MmeI	TCCRAC	4 cut(s)	241, 496, 834, 874
Mph1103I	ATGCAT	1 cut(s)	709
MseI	TTAA	3 cut(s)	30, 131, 872
MspA1I	CMGCKG	1 cut(s)	648
MspR9I	CCNGG	2 cut(s)	66, 355
MvaI	CCWGG	2 cut(s)	66, 355
MwoI	GCNNNNNNNGC	2 cut(s)	268, 997
NdeII	GATC	5 cut(s)	204, 331, 664, 739, 748
NlaIII	CATG	5 cut(s)	292, 404, 711, 879, 957
NlaIV	GGNNCC	3 cut(s)	359, 567, 845
NsiI	ATGCAT	1 cut(s)	709
NspI	RCATGY	1 cut(s)	879
NspV	TTCGAA	1 cut(s)	7
PaeR7I	CTCGAG	1 cut(s)	381
PfeI	GAWTC	3 cut(s)	176, 407, 959
PkrI	GCNGC	4 cut(s)	139, 251, 264, 828
Psp6I	CCWGG	2 cut(s)	64, 353
PspGI	CCWGG	2 cut(s)	64, 353
PspN4I	GGNNCC	3 cut(s)	359, 567, 845
PspPI	GGNCC	4 cut(s)	95, 260, 307, 843
PspXI	VCTCGAGB	1 cut(s)	381
PsuI	RGATCY	2 cut(s)	664, 748
SaqAI	TTAA	3 cut(s)	30, 131, 872
SatI	GCNGC	4 cut(s)	138, 250, 263, 827
Sau3AI	GATC	5 cut(s)	204, 331, 664, 739, 748
Sau96I	GGNCC	4 cut(s)	95, 260, 307, 843
ScrFI	CCNGG	2 cut(s)	66, 355
SduI	GDGCHC	2 cut(s)	353, 636
SexAI	ACCWGGT	1 cut(s)	353
SfaNI	GCATC	1 cut(s)	236
SfcI	CTRYAG	1 cut(s)	613
Sfr274I	CTCGAG	1 cut(s)	381
SfuI	TTCGAA	1 cut(s)	7
SinI	GGWCC	1 cut(s)	307
SlaI	CTCGAG	1 cut(s)	381
SmlI	CTYRAG	2 cut(s)	340, 381
SmoI	CTYRAG	2 cut(s)	340, 381
Sse9I	AATT	8 cut(s)	132, 146, 366, 420, 467, 480, 590, 713
SsiI	CCGC	5 cut(s)	263, 605, 648, 827, 912
SspMI	CTAG	1 cut(s)	501
StyD4I	CCNGG	2 cut(s)	64, 353
StyI	CCWWGG	1 cut(s)	76
TaiI	ACGT	2 cut(s)	131, 728
TaqI	TCGA	5 cut(s)	7, 24, 382, 636, 728
TasI	AATT	8 cut(s)	132, 146, 366, 420, 467, 480, 590, 713
TauI	GCSGC	2 cut(s)	265, 829
TfiI	GAWTC	3 cut(s)	176, 407, 959
Tru1I	TTAA	3 cut(s)	30, 131, 872
Tru9I	TTAA	3 cut(s)	30, 131, 872
TseI	GCWGC	2 cut(s)	137, 249
TspDTI	ATGAA	5 cut(s)	50, 297, 305, 920, 942
VpaK11BI	GGWCC	1 cut(s)	307
XapI	RAATTY	1 cut(s)	467
XceI	RCATGY	1 cut(s)	879
XcmI	CCANNNNNNNNNTGG	1 cut(s)	479
XhoI	CTCGAG	1 cut(s)	381
XspI	CTAG	1 cut(s)	501
ZraI	GACGTC	1 cut(s)	726
Zsp2I	ATGCAT	1 cut(s)	709

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.