Rorug04G0009200

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0017283
Physical Location & Seq
Forward (+)
1458655 .. 1459476
822 bp
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UTR
Exon/CDS
Intron
Rorug04G0009200.1

Sequence Viewer

Length: 594 bp
ATGACCAAAAAGTCCAAAACCCTCCCACTACTCGATTCTACTTTCCTCATTTCCCTCACCGTTTTCTTCTTCACAATCCATGCCACCGCAAAATCCTCAATCTTCTCAGAAGACACATCTCCATTGTCACTTGGGATCAAGCAAGAGAAGCTAAGCCACCTCCACTTCTACTTCCACGATATCGCCAGCGGTCCCAACCCAACTTCCATCTGGGTTGCCCGAGCACCCACGTCTAGCAAATCTCCGACGCTGTTTGGCTCTGTAGCTATGATCGATGATGCGTTAACTATCGGCCCCAAAAGGAGCTCCAAAGTTGTGGGAAGGGCACAAGGGTTTTATGCATCTGCCTCACAAAGTGAAAATGCTTTGATGGTGACCATGAACTTGGCTTTCACCGAAGGGAAGTTTAATGGCAGCAGTCTTACTGTTGTGGGACGAAACCCTGTGGTTGAAGCTGTAAGAGAGATGCCTATAATCGGTGGAAGTGGAGTTTTTCGGTTTTCTAGGGGATATGTTCAGGCCAAGACTATCAGGTTCAATGCAACAACCGGAGATGCTGTTGTTGAATATAATGTGTTTGTGTTCCATTATTGA

Protein Analysis

197

Amino Acids

21.41

Weight (kDa)

9.65

Isoelectric Point (pI)

40.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 52 - 195 4.9e-58 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017283)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g06650
malus_domestica MD13G1236800.v1.1 MD16G1241900.v1.1
prunus_persica Prupe.1G068800_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0398541
rosa_laevigata RLG00000009346
rosa_roxburghii Rroxscaffold_5G00343510
rosa_rugosa Rorug04G0009200
rosa_samantha Rh4AG087000 Rh4BG083700 Rh4CG095200 Rh4DG078600
rosa_wichuraiana Rw4G007260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 10
AciI CCGC 2 cut(s) 87, 189
AclWI GGATC 1 cut(s) 143
AdeI CACNNNGTG 1 cut(s) 356
AfiI CCNNNNNNNGG 1 cut(s) 476
AgsI TTSAA 3 cut(s) 452, 538, 566
AjiI CACGTC 1 cut(s) 231
AluBI AGCT 4 cut(s) 151, 266, 306, 455
AluI AGCT 4 cut(s) 151, 266, 306, 455
Alw21I GWGCWC 2 cut(s) 226, 308
AlwI GGATC 1 cut(s) 143
Ama87I CYCGRG 1 cut(s) 219
AoxI GGCC 2 cut(s) 292, 519
ApeKI GCWGC 1 cut(s) 414
AspS9I GGNCC 2 cut(s) 191, 293
AsuHPI GGTGA 3 cut(s) 49, 385, 385
AvaI CYCGRG 1 cut(s) 219
AvaII GGWCC 1 cut(s) 191
BaeGI GKGCMC 1 cut(s) 328
BanII GRGCYC 1 cut(s) 308
BbsI GAAGAC 1 cut(s) 117
Bbv12I GWGCWC 2 cut(s) 226, 308
BbvI GCAGC 1 cut(s) 426
BccI CCATC 2 cut(s) 215, 364
BfaI CTAG 2 cut(s) 234, 504
BfmI CTRYAG 1 cut(s) 261
BisI GCNGC 1 cut(s) 415
BlpI GCTNAGC 1 cut(s) 152
BlsI GCNGC 1 cut(s) 416
Bme18I GGWCC 1 cut(s) 191
BmeT110I CYCGRG 1 cut(s) 219
BmgBI CACGTC 1 cut(s) 231
BmgT120I GGNCC 2 cut(s) 191, 293
BmiI GGNNCC 2 cut(s) 193, 295
BmsI GCATC 4 cut(s) 268, 350, 456, 544
BpiI GAAGAC 1 cut(s) 117
Bpu1102I GCTNAGC 1 cut(s) 152
Bsa29I ATCGAT 1 cut(s) 273
BsaWI WCCGGW 1 cut(s) 548
BsaXI ACNNNNNCTCC 2 cut(s) 543, 573
Bsc4I CCNNNNNNNGG 1 cut(s) 476
BseCI ATCGAT 1 cut(s) 273
BseLI CCNNNNNNNGG 1 cut(s) 476
BseMII CTCAG 1 cut(s) 120
BseSI GKGCMC 1 cut(s) 328
BseXI GCAGC 1 cut(s) 426
BshFI GGCC 2 cut(s) 294, 521
BshVI ATCGAT 1 cut(s) 273
BsiHKAI GWGCWC 2 cut(s) 226, 308
BsiHKCI CYCGRG 1 cut(s) 219
BsiSI CCGG 1 cut(s) 549
BslFI GGGAC 2 cut(s) 177, 447
BslI CCNNNNNNNGG 1 cut(s) 476
BsmFI GGGAC 2 cut(s) 177, 447
BsnI GGCC 2 cut(s) 294, 521
BsoBI CYCGRG 1 cut(s) 219
Bsp1286I GDGCHC 3 cut(s) 226, 308, 328
Bsp143I GATC 2 cut(s) 135, 270
Bsp1720I GCTNAGC 1 cut(s) 152
BspACI CCGC 2 cut(s) 87, 189
BspANI GGCC 2 cut(s) 294, 521
BspCNI CTCAG 1 cut(s) 119
BspDI ATCGAT 1 cut(s) 273
BspLI GGNNCC 2 cut(s) 193, 295
BspPI GGATC 1 cut(s) 143
BssMI GATC 2 cut(s) 135, 270
Bst4CI ACNGT 2 cut(s) 61, 427
BstC8I GCNNGC 1 cut(s) 187
BstDEI CTNAG 2 cut(s) 106, 152
BstEII GGTNACC 1 cut(s) 373
BstKTI GATC 2 cut(s) 138, 273
BstMBI GATC 2 cut(s) 135, 270
BstMWI GCNNNNNNNGC 1 cut(s) 148
BstPI GGTNACC 1 cut(s) 373
BstSFI CTRYAG 1 cut(s) 261
BstSLI GKGCMC 1 cut(s) 328
BstV1I GCAGC 1 cut(s) 426
BstV2I GAAGAC 1 cut(s) 117
BstXI CCANNNNNNTGG 2 cut(s) 316, 385
Bsu15I ATCGAT 1 cut(s) 273
BsuRI GGCC 2 cut(s) 294, 521
BsuTUI ATCGAT 1 cut(s) 273
BtrI CACGTC 1 cut(s) 231
Cac8I GCNNGC 1 cut(s) 187
Cfr13I GGNCC 2 cut(s) 191, 293
ClaI ATCGAT 1 cut(s) 273
CseI GACGC 1 cut(s) 256
CviAII CATG 2 cut(s) 80, 379
CviJI RGCY 9 cut(s) 151, 156, 258, 266, 294, 306, 389, 455, 521
CviKI_1 RGCY 9 cut(s) 151, 156, 258, 266, 294, 306, 389, 455, 521
DdeI CTNAG 2 cut(s) 106, 152
DpnI GATC 2 cut(s) 137, 272
DpnII GATC 2 cut(s) 135, 270
DraIII CACNNNGTG 1 cut(s) 356
DrdI GACNNNNNNGTC 1 cut(s) 10
DseDI GACNNNNNNGTC 1 cut(s) 10
Ecl136II GAGCTC 1 cut(s) 306
Eco24I GRGCYC 1 cut(s) 308
Eco32I GATATC 1 cut(s) 181
Eco47I GGWCC 1 cut(s) 191
Eco53kI GAGCTC 1 cut(s) 306
Eco88I CYCGRG 1 cut(s) 219
Eco91I GGTNACC 1 cut(s) 373
EcoICRI GAGCTC 1 cut(s) 306
EcoO65I GGTNACC 1 cut(s) 373
EcoRV GATATC 1 cut(s) 181
EcoT22I ATGCAT 1 cut(s) 343
EcoT38I GRGCYC 1 cut(s) 308
FaeI CATG 2 cut(s) 83, 382
FaiI YATR 7 cut(s) 81, 269, 339, 380, 473, 513, 570
FaqI GGGAC 2 cut(s) 177, 447
FatI CATG 2 cut(s) 79, 378
Fnu4HI GCNGC 1 cut(s) 415
FriOI GRGCYC 1 cut(s) 308
Fsp4HI GCNGC 1 cut(s) 415
FspBI CTAG 2 cut(s) 234, 504
GluI GCNGC 1 cut(s) 415
HaeIII GGCC 2 cut(s) 294, 521
HapII CCGG 1 cut(s) 549
HgaI GACGC 1 cut(s) 256
Hin1II CATG 2 cut(s) 83, 382
HincII GTYRAC 1 cut(s) 285
HindII GTYRAC 1 cut(s) 285
HinfI GANTC 1 cut(s) 35
HpaI GTTAAC 1 cut(s) 285
HpaII CCGG 1 cut(s) 549
HphI GGTGA 3 cut(s) 49, 385, 385
Hpy166II GTNNAC 1 cut(s) 285
Hpy188I TCNGA 2 cut(s) 109, 246
Hpy8I GTNNAC 1 cut(s) 285
Hpy99I CGWCG 1 cut(s) 250
HpyAV CCTTC 2 cut(s) 315, 392
HpyCH4III ACNGT 2 cut(s) 61, 427
HpyCH4IV ACGT 1 cut(s) 230
HpyCH4V TGCA 2 cut(s) 341, 542
HpyF10VI GCNNNNNNNGC 1 cut(s) 148
HpyF3I CTNAG 2 cut(s) 106, 152
HpySE526I ACGT 1 cut(s) 230
Hsp92II CATG 2 cut(s) 83, 382
KspAI GTTAAC 1 cut(s) 285
Kzo9I GATC 2 cut(s) 135, 270
LmnI GCTCC 2 cut(s) 303, 311
LpnPI CCDG 6 cut(s) 196, 199, 456, 503, 517, 562
Lsp1109I GCAGC 1 cut(s) 426
LweI GCATC 4 cut(s) 268, 350, 456, 544
MaeI CTAG 2 cut(s) 234, 504
MaeII ACGT 1 cut(s) 230
MaeIII GTNAC 2 cut(s) 126, 373
MalI GATC 2 cut(s) 137, 272
MboI GATC 2 cut(s) 135, 270
MboII GAAGA 4 cut(s) 58, 61, 94, 122
MhlI GDGCHC 3 cut(s) 226, 308, 328
MmeI TCCRAC 1 cut(s) 269
MnlI CCTC 6 cut(s) 32, 56, 65, 106, 170, 358
Mph1103I ATGCAT 1 cut(s) 343
MseI TTAA 2 cut(s) 284, 408
MspA1I CMGCKG 1 cut(s) 189
MspI CCGG 1 cut(s) 549
MwoI GCNNNNNNNGC 1 cut(s) 148
NdeII GATC 2 cut(s) 135, 270
NlaIII CATG 2 cut(s) 83, 382
NlaIV GGNNCC 2 cut(s) 193, 295
NmuCI GTSAC 2 cut(s) 126, 373
NsiI ATGCAT 1 cut(s) 343
PfeI GAWTC 1 cut(s) 35
PkrI GCNGC 1 cut(s) 416
Psp124BI GAGCTC 1 cut(s) 308
PspEI GGTNACC 1 cut(s) 373
PspN4I GGNNCC 2 cut(s) 193, 295
PspPI GGNCC 2 cut(s) 191, 293
SacI GAGCTC 1 cut(s) 308
SaqAI TTAA 2 cut(s) 284, 408
SatI GCNGC 1 cut(s) 415
Sau3AI GATC 2 cut(s) 135, 270
Sau96I GGNCC 2 cut(s) 191, 293
SduI GDGCHC 3 cut(s) 226, 308, 328
SetI ASST 7 cut(s) 153, 162, 233, 268, 308, 457, 536
SfaNI GCATC 4 cut(s) 268, 350, 456, 544
SfcI CTRYAG 1 cut(s) 261
SinI GGWCC 1 cut(s) 191
SsiI CCGC 2 cut(s) 87, 189
SspMI CTAG 2 cut(s) 234, 504
SstI GAGCTC 1 cut(s) 308
TaaI ACNGT 2 cut(s) 61, 427
TaiI ACGT 1 cut(s) 233
TaqI TCGA 2 cut(s) 33, 273
TfiI GAWTC 1 cut(s) 35
Tru1I TTAA 2 cut(s) 284, 408
Tru9I TTAA 2 cut(s) 284, 408
TseFI GTSAC 2 cut(s) 126, 373
TseI GCWGC 1 cut(s) 414
Tsp45I GTSAC 2 cut(s) 126, 373
TspDTI ATGAA 1 cut(s) 395
VpaK11BI GGWCC 1 cut(s) 191
XcmI CCANNNNNNNNNTGG 1 cut(s) 207
XspI CTAG 2 cut(s) 234, 504
Zsp2I ATGCAT 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.