MD16G1275500.v1.1

transcriptional regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0013038
Physical Location & Seq
Forward (+)
37083340 .. 37084089
750 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1275500.v1.1.491

Sequence Viewer

Length: 750 bp
ATGGCTAAACTCGGTCTTTTCTCCCGGACCGATCTCCAGAACTTAGCTCTCTTACAACAAAATCAACACCAAACCCAGTTATCAAGCCCTAATTTGGTCCCCAAAGCCTGGATGTGGAACCCTAAGCCAGTTCAAGAACCCGAAGATGACTCATGGGAGGTGAGAGCCTTCGCGGAGGACACTGGTAATGTAATGGGCACCACATGGCCTCCAAGGTCTTACACTTGCACATTTTGTAGAAGAGAATTCCGATCCGCCCAAGCCCTAGGCGGCCACATGAATGTGCACCGCCGCGACCGGGCCCGCCTCCACCAATCCCATCCTCAGATGAACCCGGCAGCAATCTCATCAGCTTCCACTACCTCACTCATAATCCCAACTCAAGAATTTGTGAATTCAAATGGTGGATTATCCCTTGTTTACCAATTGCCAAGCCCTAGTAGTATAAATGGTGCATCCTTCAATACTGCGACAACTTGGATGAGCTCAAGTAGCACAAATGGTCATGGTGTTGAGTCATTACCATCCATTCTTCATTCAGTTTCACCCAATATTCCACCCCATGACTACTTAGTGTCAGTTGTGCCACCAGCTGGAAGAAGCAATTCTAATTCCCAATATTGTCACTCAAACAAATCATCAGCTCATGATGATCCGGAACCCTCAACGTCCGGAACAGAAAATGGCAATTTTGAAGAGCTTGATTTAGAGCTTCGGCTTGGGCATGGTCGACCGGCAATACCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C2H2_6

Protein Analysis

250

Amino Acids

27.24

Weight (kDa)

6.24

Isoelectric Point (pI)

61.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C2H2_6 PF13912 74 - 98 8.4e-06 C2H2-type zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013038)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17810 AT4G17810
fragaria_vesca FvH4_5g21910 FvH4_5g21930
malus_domestica MD06G1033300.v1.1 MD16G1275500.v1.1
prunus_persica Prupe.5G038200_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0209451
rosa_laevigata RLG00000003135
rosa_multiflora Rmu_co8408129.1_g000001 Rmu_sc0007059.1_g000004
rosa_roxburghii Rroxscaffold_3G00249450
rosa_rugosa Rorug07G0113000
rosa_samantha Rh7AG246900 Rh7BG242000 Rh7CG262500 Rh7DG253200
rosa_wichuraiana Rw7G020870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 197
AccB7I CCANNNNNTGG 2 cut(s) 108, 593
AccI GTMKAC 1 cut(s) 730
AccII CGCG 2 cut(s) 173, 294
AccIII TCCGGA 2 cut(s) 655, 671
AciI CCGC 6 cut(s) 173, 255, 270, 289, 292, 304
AclWI GGATC 2 cut(s) 246, 647
AcoI YGGCCR 1 cut(s) 271
AcsI RAATTY 3 cut(s) 245, 386, 394
AfiI CCNNNNNNNGG 5 cut(s) 94, 108, 114, 298, 593
AgsI TTSAA 4 cut(s) 134, 399, 463, 695
AjnI CCWGG 1 cut(s) 107
AluBI AGCT 7 cut(s) 47, 353, 486, 593, 644, 700, 712
AluI AGCT 7 cut(s) 47, 353, 486, 593, 644, 700, 712
Alw21I GWGCWC 2 cut(s) 288, 488
Alw44I GTGCAC 1 cut(s) 284
AlwI GGATC 2 cut(s) 246, 647
Aor13HI TCCGGA 2 cut(s) 655, 671
AoxI GGCC 3 cut(s) 206, 271, 300
ApaI GGGCCC 1 cut(s) 304
ApaLI GTGCAC 1 cut(s) 284
ApeKI GCWGC 1 cut(s) 338
ApoI RAATTY 3 cut(s) 245, 386, 394
Asp700I GAANNNNTTC 1 cut(s) 604
AspA2I CCTAGG 1 cut(s) 265
AspS9I GGNCC 4 cut(s) 27, 97, 300, 301
AsuC2I CCSGG 3 cut(s) 25, 299, 335
AsuHPI GGTGA 2 cut(s) 172, 537
AvaII GGWCC 2 cut(s) 27, 97
AvrII CCTAGG 1 cut(s) 265
BaeGI GKGCMC 3 cut(s) 200, 288, 304
BanI GGYRCC 1 cut(s) 197
BanII GRGCYC 2 cut(s) 304, 488
Bbv12I GWGCWC 2 cut(s) 288, 488
BbvI GCAGC 1 cut(s) 350
BccI CCATC 2 cut(s) 327, 532
BciT130I CCWGG 1 cut(s) 109
BcnI CCSGG 3 cut(s) 25, 299, 335
BfaI CTAG 2 cut(s) 266, 438
BisI GCNGC 3 cut(s) 271, 292, 339
BlnI CCTAGG 1 cut(s) 265
BlsI GCNGC 3 cut(s) 272, 293, 340
Bme1390I CCNGG 4 cut(s) 25, 109, 299, 335
Bme18I GGWCC 2 cut(s) 27, 97
BmgT120I GGNCC 4 cut(s) 27, 97, 300, 301
BmiI GGNNCC 5 cut(s) 99, 119, 199, 302, 660
BmrFI CCNGG 4 cut(s) 25, 109, 299, 335
BmrI ACTGGG 1 cut(s) 70
BmsI GCATC 1 cut(s) 464
BmuI ACTGGG 1 cut(s) 70
BpmI CTGGAG 1 cut(s) 20
Bpu10I CCTNAGC 1 cut(s) 123
BpuEI CTTGAG 2 cut(s) 366, 472
BpuMI CCSGG 3 cut(s) 25, 299, 335
BsaJI CCNNGG 2 cut(s) 212, 265
BsaWI WCCGGW 2 cut(s) 655, 671
BsaXI ACNNNNNCTCC 2 cut(s) 193, 223
Bsc4I CCNNNNNNNGG 5 cut(s) 94, 108, 114, 298, 593
Bse118I RCCGGY 1 cut(s) 733
Bse1I ACTGG 3 cut(s) 76, 128, 187
BseAI TCCGGA 2 cut(s) 655, 671
BseBI CCWGG 1 cut(s) 109
BseDI CCNNGG 2 cut(s) 212, 265
BseGI GGATG 5 cut(s) 117, 319, 455, 486, 524
BseLI CCNNNNNNNGG 5 cut(s) 94, 108, 114, 298, 593
BseMII CTCAG 1 cut(s) 338
BseNI ACTGG 3 cut(s) 76, 128, 187
BseSI GKGCMC 3 cut(s) 200, 288, 304
BseXI GCAGC 1 cut(s) 350
Bsh1236I CGCG 2 cut(s) 173, 294
Bsh1285I CGRYCG 2 cut(s) 298, 734
BshFI GGCC 3 cut(s) 208, 273, 302
BshNI GGYRCC 1 cut(s) 197
BsiEI CGRYCG 2 cut(s) 298, 734
BsiHKAI GWGCWC 2 cut(s) 288, 488
BsiSI CCGG 6 cut(s) 25, 298, 335, 656, 672, 734
BslFI GGGAC 1 cut(s) 83
BslI CCNNNNNNNGG 5 cut(s) 94, 108, 114, 298, 593
BsmFI GGGAC 1 cut(s) 83
BsnI GGCC 3 cut(s) 208, 273, 302
Bsp120I GGGCCC 1 cut(s) 300
Bsp1286I GDGCHC 4 cut(s) 200, 288, 304, 488
Bsp13I TCCGGA 2 cut(s) 655, 671
Bsp143I GATC 3 cut(s) 31, 251, 652
BspACI CCGC 6 cut(s) 173, 255, 270, 289, 292, 304
BspANI GGCC 3 cut(s) 208, 273, 302
BspCNI CTCAG 1 cut(s) 337
BspEI TCCGGA 2 cut(s) 655, 671
BspFNI CGCG 2 cut(s) 173, 294
BspHI TCATGA 1 cut(s) 646
BspLI GGNNCC 5 cut(s) 99, 119, 199, 302, 660
BspPI GGATC 2 cut(s) 246, 647
BspQI GCTCTTC 1 cut(s) 690
BspT107I GGYRCC 1 cut(s) 197
BsrFI RCCGGY 1 cut(s) 733
BsrI ACTGG 3 cut(s) 76, 128, 187
BssAI RCCGGY 1 cut(s) 733
BssECI CCNNGG 2 cut(s) 212, 265
BssMI GATC 3 cut(s) 31, 251, 652
BssT1I CCWWGG 2 cut(s) 212, 265
Bst2UI CCWGG 1 cut(s) 109
Bst6I CTCTTC 2 cut(s) 235, 690
BstC8I GCNNGC 1 cut(s) 304
BstDEI CTNAG 4 cut(s) 43, 123, 324, 571
BstF5I GGATG 5 cut(s) 117, 319, 455, 486, 524
BstFNI CGCG 2 cut(s) 173, 294
BstKTI GATC 3 cut(s) 34, 254, 655
BstMBI GATC 3 cut(s) 31, 251, 652
BstMCI CGRYCG 2 cut(s) 298, 734
BstMWI GCNNNNNNNGC 1 cut(s) 492
BstNI CCWGG 1 cut(s) 109
BstSCI CCNGG 4 cut(s) 23, 107, 297, 333
BstSLI GKGCMC 3 cut(s) 200, 288, 304
BstUI CGCG 2 cut(s) 173, 294
BstV1I GCAGC 1 cut(s) 350
BsuRI GGCC 3 cut(s) 208, 273, 302
BtsCI GGATG 5 cut(s) 117, 319, 455, 486, 524
BtsIMutI CAGTG 1 cut(s) 180
Cac8I GCNNGC 1 cut(s) 304
CciI TCATGA 1 cut(s) 646
Cfr10I RCCGGY 1 cut(s) 733
Cfr13I GGNCC 4 cut(s) 27, 97, 300, 301
CpoI CGGWCCG 1 cut(s) 27
CspI CGGWCCG 1 cut(s) 27
CviAII CATG 7 cut(s) 153, 204, 277, 506, 563, 647, 725
DdeI CTNAG 4 cut(s) 43, 123, 324, 571
DpnI GATC 3 cut(s) 33, 253, 654
DpnII GATC 3 cut(s) 31, 251, 652
EaeI YGGCCR 1 cut(s) 271
Eam1104I CTCTTC 2 cut(s) 235, 690
EarI CTCTTC 2 cut(s) 235, 690
EciI GGCGGA 1 cut(s) 244
Ecl136II GAGCTC 1 cut(s) 486
Eco130I CCWWGG 2 cut(s) 212, 265
Eco24I GRGCYC 2 cut(s) 304, 488
Eco47I GGWCC 2 cut(s) 27, 97
Eco53kI GAGCTC 1 cut(s) 486
EcoICRI GAGCTC 1 cut(s) 486
EcoRI GAATTC 2 cut(s) 245, 394
EcoRII CCWGG 1 cut(s) 107
EcoT14I CCWWGG 2 cut(s) 212, 265
EcoT38I GRGCYC 2 cut(s) 304, 488
ErhI CCWWGG 2 cut(s) 212, 265
FaeI CATG 7 cut(s) 156, 207, 280, 509, 566, 650, 728
FaiI YATR 9 cut(s) 154, 205, 278, 371, 446, 507, 564, 648, 726
FaqI GGGAC 1 cut(s) 83
FatI CATG 7 cut(s) 152, 203, 276, 505, 562, 646, 724
FauI CCCGC 1 cut(s) 311
FblI GTMKAC 1 cut(s) 730
Fnu4HI GCNGC 3 cut(s) 271, 292, 339
FokI GGATG 5 cut(s) 124, 306, 442, 493, 511
FriOI GRGCYC 2 cut(s) 304, 488
Fsp4HI GCNGC 3 cut(s) 271, 292, 339
FspBI CTAG 2 cut(s) 266, 438
GluI GCNGC 3 cut(s) 271, 292, 339
GsuI CTGGAG 1 cut(s) 20
HaeIII GGCC 3 cut(s) 208, 273, 302
HapII CCGG 6 cut(s) 25, 298, 335, 656, 672, 734
Hin1II CATG 7 cut(s) 156, 207, 280, 509, 566, 650, 728
HincII GTYRAC 1 cut(s) 731
HindII GTYRAC 1 cut(s) 731
HinfI GANTC 2 cut(s) 149, 515
HpaII CCGG 6 cut(s) 25, 298, 335, 656, 672, 734
HphI GGTGA 2 cut(s) 172, 537
Hpy166II GTNNAC 3 cut(s) 286, 421, 731
Hpy188I TCNGA 2 cut(s) 251, 327
Hpy188III TCNNGA 7 cut(s) 37, 134, 383, 647, 656, 672, 747
Hpy8I GTNNAC 3 cut(s) 286, 421, 731
HpyAV CCTTC 2 cut(s) 178, 469
HpyCH4IV ACGT 1 cut(s) 668
HpyCH4V TGCA 3 cut(s) 228, 286, 455
HpyF10VI GCNNNNNNNGC 1 cut(s) 492
HpyF3I CTNAG 4 cut(s) 43, 123, 324, 571
HpySE526I ACGT 1 cut(s) 668
Hsp92II CATG 7 cut(s) 156, 207, 280, 509, 566, 650, 728
Kpn2I TCCGGA 2 cut(s) 655, 671
Kzo9I GATC 3 cut(s) 31, 251, 652
LguI GCTCTTC 1 cut(s) 690
Lsp1109I GCAGC 1 cut(s) 350
LweI GCATC 1 cut(s) 464
MaeI CTAG 2 cut(s) 266, 438
MaeII ACGT 1 cut(s) 668
MaeIII GTNAC 1 cut(s) 623
MalI GATC 3 cut(s) 33, 253, 654
MboI GATC 3 cut(s) 31, 251, 652
MboII GAAGA 5 cut(s) 155, 252, 524, 609, 707
MfeI CAATTG 1 cut(s) 425
MhlI GDGCHC 4 cut(s) 200, 288, 304, 488
MluCI AATT 8 cut(s) 91, 245, 386, 394, 425, 604, 610, 688
MlyI GAGTC 2 cut(s) 143, 524
MnlI CCTC 7 cut(s) 151, 169, 219, 317, 333, 373, 673
MroI TCCGGA 2 cut(s) 655, 671
MroXI GAANNNNTTC 1 cut(s) 604
MslI CAYNNNNRTG 2 cut(s) 279, 281
MspA1I CMGCKG 1 cut(s) 593
MspI CCGG 6 cut(s) 25, 298, 335, 656, 672, 734
MspR9I CCNGG 4 cut(s) 25, 109, 299, 335
MunI CAATTG 1 cut(s) 425
MvaI CCWGG 1 cut(s) 109
MvnI CGCG 2 cut(s) 173, 294
MwoI GCNNNNNNNGC 1 cut(s) 492
NciI CCSGG 3 cut(s) 25, 299, 335
NdeII GATC 3 cut(s) 31, 251, 652
NlaIII CATG 7 cut(s) 156, 207, 280, 509, 566, 650, 728
NlaIV GGNNCC 5 cut(s) 99, 119, 199, 302, 660
NmuCI GTSAC 1 cut(s) 623
PagI TCATGA 1 cut(s) 646
PciSI GCTCTTC 1 cut(s) 690
PdmI GAANNNNTTC 1 cut(s) 604
PflMI CCANNNNNTGG 2 cut(s) 108, 593
PfoI TCCNGGA 1 cut(s) 23
PkrI GCNGC 3 cut(s) 272, 293, 340
PleI GAGTC 2 cut(s) 143, 523
PpsI GAGTC 2 cut(s) 143, 523
Psp124BI GAGCTC 1 cut(s) 488
Psp6I CCWGG 1 cut(s) 107
PspGI CCWGG 1 cut(s) 107
PspN4I GGNNCC 5 cut(s) 99, 119, 199, 302, 660
PspOMI GGGCCC 1 cut(s) 300
PspPI GGNCC 4 cut(s) 27, 97, 300, 301
PvuII CAGCTG 1 cut(s) 593
RseI CAYNNNNRTG 2 cut(s) 279, 281
Rsr2I CGGWCCG 1 cut(s) 27
RsrII CGGWCCG 1 cut(s) 27
SacI GAGCTC 1 cut(s) 488
SalI GTCGAC 1 cut(s) 729
SapI GCTCTTC 1 cut(s) 690
SatI GCNGC 3 cut(s) 271, 292, 339
Sau3AI GATC 3 cut(s) 31, 251, 652
Sau96I GGNCC 4 cut(s) 27, 97, 300, 301
SchI GAGTC 2 cut(s) 143, 524
ScrFI CCNGG 4 cut(s) 25, 109, 299, 335
SduI GDGCHC 4 cut(s) 200, 288, 304, 488
SfaNI GCATC 1 cut(s) 464
SinI GGWCC 2 cut(s) 27, 97
SmiMI CAYNNNNRTG 2 cut(s) 279, 281
SmlI CTYRAG 2 cut(s) 381, 487
SmoI CTYRAG 2 cut(s) 381, 487
Sse9I AATT 8 cut(s) 91, 245, 386, 394, 425, 604, 610, 688
SsiI CCGC 6 cut(s) 173, 255, 270, 289, 292, 304
SspI AATATT 2 cut(s) 553, 620
SspMI CTAG 2 cut(s) 266, 438
SstI GAGCTC 1 cut(s) 488
StyD4I CCNGG 4 cut(s) 23, 107, 297, 333
StyI CCWWGG 2 cut(s) 212, 265
TaiI ACGT 1 cut(s) 671
TaqI TCGA 1 cut(s) 730
TaqII GACCGA 1 cut(s) 44
TasI AATT 8 cut(s) 91, 245, 386, 394, 425, 604, 610, 688
TauI GCSGC 2 cut(s) 273, 294
TscAI CASTG 1 cut(s) 187
TseFI GTSAC 1 cut(s) 623
TseI GCWGC 1 cut(s) 338
Tsp45I GTSAC 1 cut(s) 623
TspDTI ATGAA 3 cut(s) 293, 344, 524
TspRI CASTG 1 cut(s) 187
Van91I CCANNNNNTGG 2 cut(s) 108, 593
VneI GTGCAC 1 cut(s) 284
VpaK11BI GGWCC 2 cut(s) 27, 97
XapI RAATTY 3 cut(s) 245, 386, 394
XmaJI CCTAGG 1 cut(s) 265
XmiI GTMKAC 1 cut(s) 730
XmnI GAANNNNTTC 1 cut(s) 604
XspI CTAG 2 cut(s) 266, 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.