MD17G1014600.v1.1
NAC Family

Inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0004597
Physical Location & Seq
Reverse (-)
1205421 .. 1209485
4065 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1014600.v1.1.491

Sequence Viewer

Length: 2316 bp
ATGAGATATTTTTACTTGTTAGTTTCAGTAATTGTGTCATGGGTTTTGTTCCTTCCAAGAACCCATGAATTGCAAACCTCTCAGAGCCAGGTGTTGTTGCAGTTGAAGAAGCATTTAGAGTACCCTCCCTCATTAGAAATTTGGGGAAATTACAGTGGAGACCTCTGTAACCTGTCTTCCACTGCTCATGTGAGCATTTCATGCCAGGGCAATTCAGTCACTGAGCTCAAAATTATGGGAGATTATAAGCTTGTTAATGTGAGTGGTTTCAATGGATTTGCAATTCCCAACCAGACACTGTCTGAGAATTTTGAGATTGATTCTTTTTTCACCACATTGTCAAGGTTGCCTAGTTTGAGGGTTCTTAGCTTAGTGTCGTTGGGGATTTGGGGTCCGCTTCCTGATAAGATTCATCGGTTATCTTCGCTTGAAGTCTTGGACTTGAGCTCAAATTTCATGTTTGGTTCCATTCCGGCGAAAATATCCAGAATTGTGAAACTTCAGACCCTAACACTGGAGAGCAATTATTTCAATGAAACTGTCCCTGAGTGGTTGGACTCGTTGTCGAATCTCACTGTTTTGAGCTTAAAGAACAATCGTTTGAAGGGCAAGTTTCCTTCTTCGTTATGCAGAATCAAGACACTCACTGTCATTGCTTTGTCTCACAATGAGCTCTCTGGAAAGTTACCTGATATGGCTACTTTAAGCAGCCTGCATGTCTTGGACTTGAGAGAAAATCAATTAGATTCCGAACTACCAAGGATGCCAAAAGCGTTGGTTACAGCTCTTCTTAGCAAGAACTCATTCTCCGGAAAGATTCCTGCACAATTTGGTGATTTGGGTCAGCTTCAGCACCTTGATCTCTCATTCAATTATCTTACCGGAGCACCGCCCTCTGCTTTGTTCTCTTTGTCAAACATCAGTTATCTGAATTTAGCATCCAATATGCTGAGCGGAGCATTCCCAGACAAGCTAAATTGTGGTGGAAAACTTGGGTTTGTTGATATTTCTAATAACAAGTTCATAGGTGATCTTCCGTCTTGCCTAGGCAGTCCTTCAGATGAGAGAGTTGTTCAACTGAACGGAAATTGTCTGTCGGTTGATTCTCGACACCAGCATCAGGTTTCATACTGCAGAGAAGCTCTTGCAAGGAGCAAACAATCTAGGGGAAGAGGAACTGTCGTGCTAGTTGCTGTTATCGCTGGAGCTGTTCTTGTTTTACTACTTTTGGTATTTGGGGTTCTTTTCTTTTGTCGAAGATACCGTTCTAGAAGGACGGTTGAGCACAACATATTTGCAAAGGCCGTGCCAGATAATTCACCAAATGGTTATTGCTCTGAACTCGTCGCAAACGCAAGATTCATATCTCAAGCAGCGAAGTTAGACACACACGGTGCCCCAGCGAGTCGTATATTTTCGCTTGAACAGTTGAAGGAAGCAACAGACAACTTCGATTCATCTATGTTTATGGGTGAAGGCTCTATGGGAAAGCTTTACAAAGGAAGGTTGGAAAATGGGACCTATGTTGCAATCCGATCTCTCACTATATTGAAGAAATATTCAATTCAAAACCTTAAAGTTCGGCTGGATTTGCTCTCCAAGCTTCATCATCCACACTTGGCAGGCCTCTTGGGTTATTGCATCGACACTGGTGGACACGATGATTCCAGTGGGAACAGAGTCTTCCTTGTAAATGAATTTGTCTCTGGTGGGAACTACCGTACCTATCTGTCAGAAAACTATCCGGAGAAGGTTCTCAAGTGGTCTGATAGATTGGCAATTCTCATGGGAGTTGCGAAGGCCGTGGACTTTCTTCACACCGGGGTTATTCCAGGTTGCTTCAACAACCGACTGAAAACAAACAATATATTGCTTGATGAGCATCGGATCGCAAAGTTGAGTGACTATGGAATGTCCATCATCACAGATGAAAGTGAAAAACTCGTGGCAAAGGGAGAAGGCCCGAAAACAAAATCGTGGAATAAAACAAATATGGAGGGTGATGTTTACAACTTCGGATTTATCCTACTCGAATCACTGGTTGGGCCTATCGTGAGCGGAAAAGGGGAAACATTTCTGCTAAATGAAATGGCATCGTTTGGCAGCCAAGATGGTCGAAGAAGAATTGTGGACCCGATTGTGTTGACGACTTGCTCGCAAGAGTCTTTATCGATCGTGGTATCCATAACAAAAAAATGCATATCTCCAGAAGTGGCAGCTCGTCCCTCGTTCGAGGATGTTCTTTGGAACTTGCAGTATGCAGCTCAAGTGCAAGCCACTGCTGATAGTGATCAGAAATCCGATTCTACGTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_8 Pkinase_Tyr

Protein Analysis

772

Amino Acids

85.05

Weight (kDa)

8.5

Isoelectric Point (pI)

32.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 111 - 240 3.3e-10 Leucine-rich repeat region
LRR_8 PF13855 166 - 225 5e-09 Leucine rich repeat
LRR_8 PF13855 264 - 317 1.5e-07 Leucine rich repeat
LRR_8 PF13855 282 - 341 2.8e-07 Leucine rich repeat
Pkinase PF00069 488 - 748 6.2e-16 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 490 - 749 8.8e-21 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 246
AccB1I GGYRCC 1 cut(s) 1394
AccBSI CCGCTC 2 cut(s) 954, 2058
AccIII TCCGGA 2 cut(s) 809, 1744
AciI CCGC 4 cut(s) 395, 890, 954, 2058
AclWI GGATC 1 cut(s) 1895
AcsI RAATTY 5 cut(s) 138, 307, 451, 931, 1697
AcuI CTGAAG 3 cut(s) 485, 833, 1041
AdeI CACNNNGTG 1 cut(s) 1394
AfaI GTAC 2 cut(s) 122, 1723
AfiI CCNNNNNNNGG 2 cut(s) 514, 1120
AhdI GACNNNNNGTC 1 cut(s) 562
AjnI CCWGG 3 cut(s) 87, 204, 1831
AjuI GAANNNNNNNTTGG 2 cut(s) 2025, 2057
AloI GAACNNNNNNTCC 2 cut(s) 791, 823
Alw21I GWGCWC 5 cut(s) 228, 449, 675, 889, 1287
Alw26I GTCTC 3 cut(s) 153, 666, 1708
AlwI GGATC 1 cut(s) 1895
AlwNI CAGNNNCTG 2 cut(s) 221, 298
Aor13HI TCCGGA 2 cut(s) 809, 1744
AoxI GGCC 5 cut(s) 1302, 1624, 1800, 1960, 2045
ApeKI GCWGC 5 cut(s) 708, 1373, 2103, 2216, 2261
ApoI RAATTY 5 cut(s) 138, 307, 451, 931, 1697
Asp700I GAANNNNTTC 2 cut(s) 803, 2073
AspA2I CCTAGG 1 cut(s) 1045
AspS9I GGNCC 5 cut(s) 392, 1518, 1961, 2045, 2131
AsuC2I CCSGG 1 cut(s) 1822
AsuHPI GGTGA 6 cut(s) 322, 845, 1040, 1311, 1484, 2012
AvaII GGWCC 3 cut(s) 392, 1518, 2131
AvrII CCTAGG 1 cut(s) 1045
BaeGI GKGCMC 1 cut(s) 1399
BaeI ACNNNNGTAYC 2 cut(s) 1705, 1738
BanI GGYRCC 1 cut(s) 1394
BanII GRGCYC 3 cut(s) 228, 449, 675
BarI GAAGNNNNNNTAC 2 cut(s) 160, 192
BauI CACGAG 1 cut(s) 1943
BbsI GAAGAC 2 cut(s) 168, 1675
Bbv12I GWGCWC 5 cut(s) 228, 449, 675, 889, 1287
BbvI GCAGC 5 cut(s) 720, 1385, 2115, 2228, 2273
BccI CCATC 2 cut(s) 1925, 2105
BceAI ACGGC 2 cut(s) 1289, 1787
BciT130I CCWGG 3 cut(s) 89, 206, 1833
BciVI GTATCC 1 cut(s) 2191
BclI TGATCA 1 cut(s) 2290
BcnI CCSGG 1 cut(s) 1822
BcoDI GTCTC 3 cut(s) 153, 666, 1708
BfaI CTAG 5 cut(s) 351, 1046, 1164, 1187, 1269
BfmI CTRYAG 1 cut(s) 1132
BfuI GTATCC 1 cut(s) 2191
BisI GCNGC 5 cut(s) 709, 1374, 2104, 2217, 2262
BlnI CCTAGG 1 cut(s) 1045
BlpI GCTNAGC 1 cut(s) 950
BlsI GCNGC 5 cut(s) 710, 1375, 2105, 2218, 2263
Bme1390I CCNGG 4 cut(s) 89, 206, 1822, 1833
Bme18I GGWCC 3 cut(s) 392, 1518, 2131
BmeRI GACNNNNNGTC 1 cut(s) 562
BmgT120I GGNCC 5 cut(s) 392, 1518, 1961, 2045, 2131
BmiI GGNNCC 5 cut(s) 393, 466, 1396, 1519, 2133
BmrFI CCNGG 4 cut(s) 89, 206, 1822, 1833
BmsI GCATC 6 cut(s) 753, 947, 1126, 1650, 1891, 2102
BpiI GAAGAC 2 cut(s) 168, 1675
BpmI CTGGAG 3 cut(s) 536, 1224, 2190
Bpu1102I GCTNAGC 1 cut(s) 950
BpuEI CTTGAG 5 cut(s) 463, 748, 1353, 1742, 2250
BpuMI CCSGG 1 cut(s) 1822
Bsa29I ATCGAT 1 cut(s) 2171
BsaBI GATNNNNATC 3 cut(s) 1363, 1881, 2289
BsaI GGTCTC 1 cut(s) 153
BsaJI CCNNGG 5 cut(s) 205, 758, 1045, 1803, 1821
BsaWI WCCGGW 3 cut(s) 809, 881, 1744
BsaXI ACNNNNNCTCC 2 cut(s) 791, 821
Bsc4I CCNNNNNNNGG 2 cut(s) 514, 1120
Bse1I ACTGG 4 cut(s) 519, 1654, 1668, 2043
Bse3DI GCAATG 1 cut(s) 651
Bse8I GATNNNNATC 3 cut(s) 1363, 1881, 2289
BseAI TCCGGA 2 cut(s) 809, 1744
BseBI CCWGG 3 cut(s) 89, 206, 1833
BseCI ATCGAT 1 cut(s) 2171
BseDI CCNNGG 5 cut(s) 205, 758, 1045, 1803, 1821
BseGI GGATG 4 cut(s) 768, 938, 1609, 2242
BseJI GATNNNNATC 3 cut(s) 1363, 1881, 2289
BseLI CCNNNNNNNGG 2 cut(s) 514, 1120
BseMI GCAATG 1 cut(s) 651
BseMII CTCAG 5 cut(s) 95, 213, 294, 537, 941
BseNI ACTGG 4 cut(s) 519, 1654, 1668, 2043
BseSI GKGCMC 1 cut(s) 1399
BseXI GCAGC 5 cut(s) 720, 1385, 2115, 2228, 2273
BseYI CCCAGC 1 cut(s) 1399
BsgI GTGCAG 1 cut(s) 807
Bsh1285I CGRYCG 1 cut(s) 2175
BshFI GGCC 5 cut(s) 1304, 1626, 1802, 1962, 2047
BshNI GGYRCC 1 cut(s) 1394
BshVI ATCGAT 1 cut(s) 2171
BsiEI CGRYCG 1 cut(s) 2175
BsiHKAI GWGCWC 5 cut(s) 228, 449, 675, 889, 1287
BsiSI CCGG 5 cut(s) 473, 810, 882, 1745, 1821
BslFI GGGAC 3 cut(s) 527, 1531, 2208
BslI CCNNNNNNNGG 2 cut(s) 514, 1120
BsmAI GTCTC 3 cut(s) 153, 666, 1708
BsmFI GGGAC 3 cut(s) 527, 1531, 2208
BsmI GAATGC 1 cut(s) 959
BsnI GGCC 5 cut(s) 1304, 1626, 1802, 1962, 2047
Bso31I GGTCTC 1 cut(s) 153
Bsp1286I GDGCHC 6 cut(s) 228, 449, 675, 889, 1287, 1399
Bsp13I TCCGGA 2 cut(s) 809, 1744
Bsp143I GATC 6 cut(s) 859, 1030, 1535, 1887, 2172, 2290
Bsp1720I GCTNAGC 1 cut(s) 950
BspACI CCGC 4 cut(s) 395, 890, 954, 2058
BspANI GGCC 5 cut(s) 1304, 1626, 1802, 1962, 2047
BspCNI CTCAG 5 cut(s) 94, 214, 295, 538, 942
BspDI ATCGAT 1 cut(s) 2171
BspEI TCCGGA 2 cut(s) 809, 1744
BspLI GGNNCC 5 cut(s) 393, 466, 1396, 1519, 2133
BspMAI CTGCAG 1 cut(s) 1136
BspPI GGATC 1 cut(s) 1895
BspQI GCTCTTC 1 cut(s) 792
BspT107I GGYRCC 1 cut(s) 1394
BspTNI GGTCTC 1 cut(s) 153
BsrBI CCGCTC 2 cut(s) 954, 2058
BsrDI GCAATG 1 cut(s) 651
BsrI ACTGG 4 cut(s) 519, 1654, 1668, 2043
BssECI CCNNGG 5 cut(s) 205, 758, 1045, 1803, 1821
BssMI GATC 6 cut(s) 859, 1030, 1535, 1887, 2172, 2290
BssSI CACGAG 1 cut(s) 1943
BssT1I CCWWGG 2 cut(s) 758, 1045
Bst2BI CACGAG 1 cut(s) 1943
Bst2UI CCWGG 3 cut(s) 89, 206, 1833
Bst6I CTCTTC 2 cut(s) 792, 1165
BstAPI GCANNNNNTGC 1 cut(s) 201
BstC8I GCNNGC 4 cut(s) 713, 1624, 2156, 2274
BstDEI CTNAG 8 cut(s) 81, 222, 303, 365, 370, 546, 791, 950
BstDSI CCRYGG 1 cut(s) 1803
BstF5I GGATG 4 cut(s) 768, 938, 1609, 2242
BstKTI GATC 6 cut(s) 862, 1033, 1538, 1890, 2175, 2293
BstMAI GTCTC 3 cut(s) 153, 666, 1708
BstMBI GATC 6 cut(s) 859, 1030, 1535, 1887, 2172, 2290
BstMCI CGRYCG 1 cut(s) 2175
BstMWI GCNNNNNNNGC 5 cut(s) 201, 1199, 1591, 1600, 1879
BstNI CCWGG 3 cut(s) 89, 206, 1833
BstNSI RCATGY 1 cut(s) 719
BstSCI CCNGG 4 cut(s) 87, 204, 1820, 1831
BstSFI CTRYAG 1 cut(s) 1132
BstSLI GKGCMC 1 cut(s) 1399
BstV1I GCAGC 5 cut(s) 720, 1385, 2115, 2228, 2273
BstV2I GAAGAC 2 cut(s) 168, 1675
Bsu15I ATCGAT 1 cut(s) 2171
BsuI GTATCC 1 cut(s) 2191
BsuRI GGCC 5 cut(s) 1304, 1626, 1802, 1962, 2047
BsuTUI ATCGAT 1 cut(s) 2171
BtgI CCRYGG 1 cut(s) 1803
BtsCI GGATG 4 cut(s) 768, 938, 1609, 2242
BtsI GCAGTG 2 cut(s) 180, 2277
Cac8I GCNNGC 4 cut(s) 713, 1624, 2156, 2274
CaiI CAGNNNCTG 2 cut(s) 221, 298
Cfr13I GGNCC 5 cut(s) 392, 1518, 1961, 2045, 2131
ClaI ATCGAT 1 cut(s) 2171
Csp6I GTAC 2 cut(s) 121, 1722
CviAII CATG 7 cut(s) 39, 65, 188, 201, 457, 716, 1786
CviQI GTAC 2 cut(s) 121, 1722
DdeI CTNAG 8 cut(s) 81, 222, 303, 365, 370, 546, 791, 950
DpnI GATC 6 cut(s) 861, 1032, 1537, 1889, 2174, 2292
DpnII GATC 6 cut(s) 859, 1030, 1535, 1887, 2172, 2290
DraIII CACNNNGTG 1 cut(s) 1394
DriI GACNNNNNGTC 1 cut(s) 562
Eam1104I CTCTTC 2 cut(s) 792, 1165
Eam1105I GACNNNNNGTC 1 cut(s) 562
EarI CTCTTC 2 cut(s) 792, 1165
Ecl136II GAGCTC 3 cut(s) 226, 447, 673
Eco130I CCWWGG 2 cut(s) 758, 1045
Eco147I AGGCCT 1 cut(s) 1626
Eco24I GRGCYC 3 cut(s) 228, 449, 675
Eco31I GGTCTC 1 cut(s) 153
Eco47I GGWCC 3 cut(s) 392, 1518, 2131
Eco53kI GAGCTC 3 cut(s) 226, 447, 673
Eco57I CTGAAG 3 cut(s) 485, 833, 1041
EcoICRI GAGCTC 3 cut(s) 226, 447, 673
EcoO109I RGGNCCY 1 cut(s) 1518
EcoRII CCWGG 3 cut(s) 87, 204, 1831
EcoT14I CCWWGG 2 cut(s) 758, 1045
EcoT22I ATGCAT 1 cut(s) 2201
EcoT38I GRGCYC 3 cut(s) 228, 449, 675
ErhI CCWWGG 2 cut(s) 758, 1045
FaeI CATG 7 cut(s) 42, 68, 191, 204, 460, 719, 1789
FaqI GGGAC 3 cut(s) 527, 1531, 2208
FatI CATG 7 cut(s) 38, 64, 187, 200, 456, 715, 1785
FbaI TGATCA 1 cut(s) 2290
Fnu4HI GCNGC 5 cut(s) 709, 1374, 2104, 2217, 2262
FokI GGATG 4 cut(s) 775, 925, 1596, 2249
FriOI GRGCYC 3 cut(s) 228, 449, 675
Fsp4HI GCNGC 5 cut(s) 709, 1374, 2104, 2217, 2262
FspBI CTAG 5 cut(s) 351, 1046, 1164, 1187, 1269
GluI GCNGC 5 cut(s) 709, 1374, 2104, 2217, 2262
GsaI CCCAGC 1 cut(s) 1403
GsuI CTGGAG 3 cut(s) 536, 1224, 2190
HaeIII GGCC 5 cut(s) 1304, 1626, 1802, 1962, 2047
HapII CCGG 5 cut(s) 473, 810, 882, 1745, 1821
Hin1II CATG 7 cut(s) 42, 68, 191, 204, 460, 719, 1789
HincII GTYRAC 1 cut(s) 2145
HindII GTYRAC 1 cut(s) 2145
HindIII AAGCTT 3 cut(s) 248, 1490, 1601
HpaII CCGG 5 cut(s) 473, 810, 882, 1745, 1821
HphI GGTGA 6 cut(s) 322, 845, 1040, 1311, 1484, 2012
Hpy166II GTNNAC 5 cut(s) 1655, 1807, 2008, 2131, 2145
Hpy8I GTNNAC 5 cut(s) 1655, 1807, 2008, 2131, 2145
Hpy99I CGWCG 2 cut(s) 1349, 2314
HpyCH4IV ACGT 1 cut(s) 2309
HpyF10VI GCNNNNNNNGC 5 cut(s) 201, 1199, 1591, 1600, 1879
HpyF3I CTNAG 8 cut(s) 81, 222, 303, 365, 370, 546, 791, 950
HpySE526I ACGT 1 cut(s) 2309
Hsp92II CATG 7 cut(s) 42, 68, 191, 204, 460, 719, 1789
Kpn2I TCCGGA 2 cut(s) 809, 1744
Ksp22I TGATCA 1 cut(s) 2290
Kzo9I GATC 6 cut(s) 859, 1030, 1535, 1887, 2172, 2290
LguI GCTCTTC 1 cut(s) 792
LmnI GCTCC 4 cut(s) 884, 956, 1152, 1205
Lsp1109I GCAGC 5 cut(s) 720, 1385, 2115, 2228, 2273
LweI GCATC 6 cut(s) 753, 947, 1126, 1650, 1891, 2102
MaeI CTAG 5 cut(s) 351, 1046, 1164, 1187, 1269
MaeII ACGT 1 cut(s) 2309
MaeIII GTNAC 5 cut(s) 167, 217, 684, 778, 1901
MalI GATC 6 cut(s) 861, 1032, 1537, 1889, 2174, 2292
MbiI CCGCTC 2 cut(s) 954, 2058
MboI GATC 6 cut(s) 859, 1030, 1535, 1887, 2172, 2290
MhlI GDGCHC 6 cut(s) 228, 449, 675, 889, 1287, 1399
MlyI GAGTC 4 cut(s) 551, 1414, 1689, 2171
MmeI TCCRAC 2 cut(s) 534, 1488
Mph1103I ATGCAT 1 cut(s) 2201
MroI TCCGGA 2 cut(s) 809, 1744
MroXI GAANNNNTTC 2 cut(s) 803, 2073
MseI TTAA 4 cut(s) 255, 587, 704, 1575
MspI CCGG 5 cut(s) 473, 810, 882, 1745, 1821
MspR9I CCNGG 4 cut(s) 89, 206, 1822, 1833
Mva1269I GAATGC 1 cut(s) 959
MvaI CCWGG 3 cut(s) 89, 206, 1833
MwoI GCNNNNNNNGC 5 cut(s) 201, 1199, 1591, 1600, 1879
NciI CCSGG 1 cut(s) 1822
NdeII GATC 6 cut(s) 859, 1030, 1535, 1887, 2172, 2290
NlaIII CATG 7 cut(s) 42, 68, 191, 204, 460, 719, 1789
NlaIV GGNNCC 5 cut(s) 393, 466, 1396, 1519, 2133
NmuCI GTSAC 2 cut(s) 217, 1901
NsiI ATGCAT 1 cut(s) 2201
NspI RCATGY 1 cut(s) 719
PceI AGGCCT 1 cut(s) 1626
PciSI GCTCTTC 1 cut(s) 792
PcsI WCGNNNNNNNCGW 1 cut(s) 1261
PctI GAATGC 1 cut(s) 959
PdmI GAANNNNTTC 2 cut(s) 803, 2073
PflFI GACNNNGTC 1 cut(s) 298
PkrI GCNGC 5 cut(s) 710, 1375, 2105, 2218, 2263
Ple19I CGATCG 1 cut(s) 2175
PleI GAGTC 4 cut(s) 551, 1413, 1688, 2170
PpsI GAGTC 4 cut(s) 551, 1413, 1688, 2170
PpuMI RGGWCCY 1 cut(s) 1518
PsiI TTATAA 1 cut(s) 246
Psp124BI GAGCTC 3 cut(s) 228, 449, 675
Psp5II RGGWCCY 1 cut(s) 1518
Psp6I CCWGG 3 cut(s) 87, 204, 1831
PspFI CCCAGC 1 cut(s) 1399
PspGI CCWGG 3 cut(s) 87, 204, 1831
PspN4I GGNNCC 5 cut(s) 393, 466, 1396, 1519, 2133
PspPI GGNCC 5 cut(s) 392, 1518, 1961, 2045, 2131
PspPPI RGGWCCY 1 cut(s) 1518
PsrI GAACNNNNNNTAC 2 cut(s) 1224, 1256
PstI CTGCAG 1 cut(s) 1136
PstNI CAGNNNCTG 2 cut(s) 221, 298
PsyI GACNNNGTC 1 cut(s) 298
PvuI CGATCG 1 cut(s) 2175
RsaI GTAC 2 cut(s) 122, 1723
RsaNI GTAC 2 cut(s) 121, 1722
SacI GAGCTC 3 cut(s) 228, 449, 675
SapI GCTCTTC 1 cut(s) 792
SaqAI TTAA 4 cut(s) 255, 587, 704, 1575
SatI GCNGC 5 cut(s) 709, 1374, 2104, 2217, 2262
Sau3AI GATC 6 cut(s) 859, 1030, 1535, 1887, 2172, 2290
Sau96I GGNCC 5 cut(s) 392, 1518, 1961, 2045, 2131
SchI GAGTC 4 cut(s) 551, 1414, 1689, 2171
ScrFI CCNGG 4 cut(s) 89, 206, 1822, 1833
SduI GDGCHC 6 cut(s) 228, 449, 675, 889, 1287, 1399
SfaNI GCATC 6 cut(s) 753, 947, 1126, 1650, 1891, 2102
SfcI CTRYAG 1 cut(s) 1132
SinI GGWCC 3 cut(s) 392, 1518, 2131
SmlI CTYRAG 5 cut(s) 442, 727, 1368, 1757, 2265
SmoI CTYRAG 5 cut(s) 442, 727, 1368, 1757, 2265
SseBI AGGCCT 1 cut(s) 1626
SsiI CCGC 4 cut(s) 395, 890, 954, 2058
SspI AATATT 1 cut(s) 1559
SspMI CTAG 5 cut(s) 351, 1046, 1164, 1187, 1269
SstI GAGCTC 3 cut(s) 228, 449, 675
StuI AGGCCT 1 cut(s) 1626
StyD4I CCNGG 4 cut(s) 87, 204, 1820, 1831
StyI CCWWGG 2 cut(s) 758, 1045
TaiI ACGT 1 cut(s) 2312
TaqI TCGA 9 cut(s) 566, 1108, 1255, 1452, 1644, 2031, 2116, 2171, 2232
Tru1I TTAA 4 cut(s) 255, 587, 704, 1575
Tru9I TTAA 4 cut(s) 255, 587, 704, 1575
TseFI GTSAC 2 cut(s) 217, 1901
TseI GCWGC 5 cut(s) 708, 1373, 2103, 2216, 2261
Tsp45I GTSAC 2 cut(s) 217, 1901
TspGWI ACGGA 2 cut(s) 1026, 1098
Tth111I GACNNNGTC 1 cut(s) 298
VpaK11BI GGWCC 3 cut(s) 392, 1518, 2131
XapI RAATTY 5 cut(s) 138, 307, 451, 931, 1697
XbaI TCTAGA 1 cut(s) 1268
XceI RCATGY 1 cut(s) 719
XmaJI CCTAGG 1 cut(s) 1045
XmnI GAANNNNTTC 2 cut(s) 803, 2073
XspI CTAG 5 cut(s) 351, 1046, 1164, 1187, 1269
Zsp2I ATGCAT 1 cut(s) 2201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.