Rroxscaffold_2G00077610
NAC Family

Inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004597
Physical Location & Seq
Reverse (-)
1122579 .. 1127308
4730 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00077610.1

Sequence Viewer

Length: 2325 bp
ATGGGGCGTTTTTACTTGTTGTTTTCAGTGATTGTGTTGTGGGTTTTGTTCCTGCCGAGTACCCATGAGTTGCAAACCTCTCAGAGCCAAGTGTTATTGCAGCTGAGGAAACATTTAGAGTTCCCTTCCCCATTAGGAGTTTTGGAGAATTACACTGGAGACCTCTGTAACCTATCTTCCTCTGCACAGATGAGCATTTCATGCCAGGAAAATTCTATCACTGAGCTCAAAGTCATGGGAGATAAGCTTTTTAATGTCACCGAGTTCAATGGATTTGCAATTCCCAATAAAACTCTTTCTGAGAAGTTCTCCATTGATACTTTTGTGACCACATTGTCAAGGTTGCCTAGCTTGAGGGTTCTTAGCTTAGTGTCTTTGGGGATTTGGGGACCACTTCCTGATAAGATTCATAGGTTATCTTCGCTTGAAGTCTTAGACCTGAGCTCAAATTTCATATTTGGTTCAATTCCCCCTAAGATATCCACAATGGTCAAGCTTCACACTCTAAGCCTGGAGGCCAATTATTTCAATGAGACTGTTCCTGATTGGTTGGACTCGTTATCAAACCTCACCATTTTGTGCTTGAAGAACAATCAGCTCAAGGGTCAGTTTCCTTCTTCAATATGCAGAATTAAGACACTCACTGACCTTGCTCTGTCTCACAATGAGCTTTCTGGAAAATTACCTGACATGAGTACTTTAACCAGCCTGCATGTATTGGATATGAGAGAAAATGATTTAGATTCTGAACTACCAGTGATGCCGAAAGGATTGGTTACAGTACTTCTTAGCAAGAACTCGTTCTCAGGAAAGATTCCGGTGCAACTTGGTGATTTGAGTCAGCTTCAACACCTTGATCTATCATTCAATTATCTCAGTGGAGCACCACCCTCTAACTTGTTCTCTTTGCCAAAAATCAGTTATCTGAATTTAGCATCCAATATGCTGAGTGGACCATTTCCAAACGGTCTAAATTGTGGTGGCAAACTTGGTTTTGTTGATATTTCTAATAACAAGTTAGCAGGGGATCTTCCTTCTTGCCTGGGTAGTACCTCAGATGAGAGAGTTGTTGAACTCAGTGGTAACTGTTTGGCGAATGCTACCCAACACCAGCATGACCATTCATATTGTACAAAGGCTCTTGCAAGGAGAAAACAATCTAGTGTGAGAGAGATTGTGGTGCTAGTTGCTGTTGCCATCGGAGCTCTTCTTGTTTTAGTACTTTCTGTACTTGCGGTTATTTGCTTGTGTAGAAGATACCGTTCAAGGAAGTCTGAGCATAACATATTTGCAAAAGCCGTGCCAGATAATTCACCAAATGATCTTTGCTCGGACATCGTTACAAATGCTAGATATATATCTCAAGCAGCGAAATTAGAGACACATGGTGCCCCAGTATGTCGATTGTTTTCACTTGAAGAGTTGAAGGAAGCAACAAACGACTTTGATTTCTCAATGTTTTTGGGTGAAGGCTCTATGGGAAGGCTTTACAAAGGCAAGTTGGAAAATGGGACCTCAGTTGCAATCAGATCTCTGGCTATATCAAAAAAATGTTCAACTAACAACCTTAAAGTTCAGCTGGATTCACTCTCAAAGCTTCACCATCCTCACTTGGTTGGCCTGTTGGGTCATTGCATTGACAGTAGTGGACAAGATGATTCTGGTGGCAAAAGACTTTTCCTTGTATATGAATACATCTCTAGTGGGAATTACCGTACCTATCTTTCAGAAAACTATCCAGAGAAGGTTCTCAAGTGGCCTGATAGATTGGCAATTCTAATAGGAGTTGCAAAAGCAGTCCATTTTCTACACACTGGGGTTATTCCAGGTTCTTTCAACAATCGATTGAAAACAAACAACATATTGCTAGATGAGCATCGGATTCCAAAGCTGAGTGACTATGGGATGTCCCTCATCACAGAAGAAATTGAAAAACCTGAGGCATGTTTTTCTTATGCAAAGGGAGAAGGCCCAAAATCAAGGCGTAAAAAGGATCTGGAGGGTGATGTTTACAGCTTTGGATTTGTACTGCTTGAATCACTCGTCGGACCTATAGTAAGTGGAAAAGGGGAAACCTTTCTGCTGAATGAAATGGCATCATTTGGGAGCCAAGATGGTCGAAGAAGAATTGTGGATCCAGTTGTGCTGACAACTTGCTCACAAGAGTCGTTATCCATTGTGGTATCGATCACAAAGAAATGCATATCTCCAGAAGTATCATCACGTCCCTCTTTCGAAGACGTGCTTTGGAACTTGCAGTATGCAGCTCAAGTCCAGGCCACTGCTGATGCTGATCAAAAATCCGATGCTACATCCGTGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_8 Pkinase_Tyr

Protein Analysis

774

Amino Acids

85.09

Weight (kDa)

6.83

Isoelectric Point (pI)

38.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 110 - 317 1.5e-12 Leucine-rich repeat region
LRR_8 PF13855 165 - 224 3.8e-07 Leucine rich repeat
LRR_8 PF13855 257 - 316 1.1e-06 Leucine rich repeat
LRR_8 PF13855 281 - 340 7.6e-06 Leucine rich repeat
Pkinase PF00069 485 - 749 3.3e-18 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 486 - 750 1.2e-21 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 334
AccB1I GGYRCC 1 cut(s) 1388
AciI CCGC 1 cut(s) 1235
AclWI GGATC 4 cut(s) 1035, 2001, 2129, 2142
AcsI RAATTY 3 cut(s) 211, 448, 928
AdeI CACNNNGTG 1 cut(s) 1388
AfaI GTAC 9 cut(s) 61, 697, 783, 1051, 1132, 1221, 1230, 1717, 2028
AjiI CACGTC 2 cut(s) 2225, 2242
AjnI CCWGG 5 cut(s) 204, 510, 1041, 1825, 2274
Alw21I GWGCWC 4 cut(s) 228, 446, 886, 1207
Alw26I GTCTC 4 cut(s) 153, 527, 663, 1373
AlwI GGATC 4 cut(s) 1035, 2001, 2129, 2142
AoxI GGCC 5 cut(s) 516, 1618, 1757, 1969, 2277
ApeKI GCWGC 3 cut(s) 100, 1367, 2264
ApoI RAATTY 3 cut(s) 211, 448, 928
Asp700I GAANNNNTTC 2 cut(s) 800, 2076
AspS9I GGNCC 5 cut(s) 389, 953, 1512, 1970, 2048
AsuHPI GGTGA 7 cut(s) 250, 562, 842, 1305, 1478, 1592, 2015
AsuII TTCGAA 1 cut(s) 2235
AvaII GGWCC 4 cut(s) 389, 953, 1512, 2048
AxyI CCTNAGG 1 cut(s) 1938
BaeGI GKGCMC 1 cut(s) 1393
BaeI ACNNNNGTAYC 2 cut(s) 309, 342
BamHI GGATCC 1 cut(s) 2134
BanI GGYRCC 1 cut(s) 1388
BanII GRGCYC 3 cut(s) 228, 446, 1207
BarI GAAGNNNNNNTAC 2 cut(s) 160, 192
BbsI GAAGAC 1 cut(s) 2244
Bbv12I GWGCWC 4 cut(s) 228, 446, 886, 1207
BbvCI CCTCAGC 1 cut(s) 104
BbvI GCAGC 3 cut(s) 112, 1379, 2276
BccI CCATC 3 cut(s) 1205, 1611, 2108
BceAI ACGGC 1 cut(s) 1283
BciT130I CCWGG 5 cut(s) 206, 512, 1043, 1827, 2276
BclI TGATCA 1 cut(s) 2293
BcoDI GTCTC 4 cut(s) 153, 527, 663, 1373
BfaI CTAG 6 cut(s) 348, 1161, 1184, 1350, 1701, 1868
BfmI CTRYAG 1 cut(s) 2052
BglII AGATCT 1 cut(s) 1529
BisI GCNGC 3 cut(s) 101, 1368, 2265
BlsI GCNGC 3 cut(s) 102, 1369, 2266
BmcAI AGTACT 3 cut(s) 697, 783, 1221
Bme1390I CCNGG 5 cut(s) 206, 512, 1043, 1827, 2276
Bme18I GGWCC 4 cut(s) 389, 953, 1512, 2048
BmgBI CACGTC 2 cut(s) 2225, 2242
BmgT120I GGNCC 5 cut(s) 389, 953, 1512, 1970, 2048
BmiI GGNNCC 5 cut(s) 390, 1390, 1513, 2108, 2136
BmrFI CCNGG 5 cut(s) 206, 512, 1043, 1827, 2276
BmrI ACTGGG 2 cut(s) 1388, 1824
BmsI GCATC 6 cut(s) 750, 944, 1885, 2105, 2278, 2296
BmuI ACTGGG 2 cut(s) 1388, 1824
BpiI GAAGAC 1 cut(s) 2244
BplI GAGNNNNNCTC 2 cut(s) 293, 325
BpmI CTGGAG 4 cut(s) 177, 533, 2018, 2193
Bpu10I CCTNAGC 2 cut(s) 104, 440
Bpu14I TTCGAA 1 cut(s) 2235
BpuEI CTTGAG 5 cut(s) 373, 584, 1347, 1736, 2253
Bsa29I ATCGAT 2 cut(s) 1843, 2186
BsaBI GATNNNNATC 3 cut(s) 1357, 1875, 2292
BsaI GGTCTC 1 cut(s) 153
BsaJI CCNNGG 1 cut(s) 1042
BsaWI WCCGGW 1 cut(s) 817
Bse1I ACTGG 5 cut(s) 160, 755, 1394, 1819, 2138
Bse21I CCTNAGG 1 cut(s) 1938
Bse3DI GCAATG 1 cut(s) 1630
Bse8I GATNNNNATC 3 cut(s) 1357, 1875, 2292
BseBI CCWGG 5 cut(s) 206, 512, 1043, 1827, 2276
BseCI ATCGAT 2 cut(s) 1843, 2186
BseDI CCNNGG 1 cut(s) 1042
BseGI GGATG 4 cut(s) 935, 1603, 1911, 2312
BseJI GATNNNNATC 3 cut(s) 1357, 1875, 2292
BseMI GCAATG 1 cut(s) 1630
BseNI ACTGG 5 cut(s) 160, 755, 1394, 1819, 2138
BseSI GKGCMC 1 cut(s) 1393
BseXI GCAGC 3 cut(s) 112, 1379, 2276
BsgI GTGCAG 1 cut(s) 168
BshFI GGCC 5 cut(s) 518, 1620, 1759, 1971, 2279
BshNI GGYRCC 1 cut(s) 1388
BshVI ATCGAT 2 cut(s) 1843, 2186
BsiHKAI GWGCWC 4 cut(s) 228, 446, 886, 1207
BsiSI CCGG 1 cut(s) 818
BslFI GGGAC 4 cut(s) 402, 1525, 1894, 2211
BsmAI GTCTC 4 cut(s) 153, 527, 663, 1373
BsmFI GGGAC 4 cut(s) 402, 1525, 1894, 2211
BsmI GAATGC 1 cut(s) 1102
BsnI GGCC 5 cut(s) 518, 1620, 1759, 1971, 2279
Bso31I GGTCTC 1 cut(s) 153
Bsp119I TTCGAA 1 cut(s) 2235
Bsp1286I GDGCHC 5 cut(s) 228, 446, 886, 1207, 1393
Bsp1407I TGTACA 1 cut(s) 1130
Bsp143I GATC 8 cut(s) 856, 1027, 1321, 1529, 1993, 2134, 2187, 2293
BspACI CCGC 1 cut(s) 1235
BspANI GGCC 5 cut(s) 518, 1620, 1759, 1971, 2279
BspDI ATCGAT 2 cut(s) 1843, 2186
BspLI GGNNCC 5 cut(s) 390, 1390, 1513, 2108, 2136
BspPI GGATC 4 cut(s) 1035, 2001, 2129, 2142
BspQI GCTCTTC 1 cut(s) 1212
BspT104I TTCGAA 1 cut(s) 2235
BspT107I GGYRCC 1 cut(s) 1388
BspTNI GGTCTC 1 cut(s) 153
BsrDI GCAATG 1 cut(s) 1630
BsrGI TGTACA 1 cut(s) 1130
BsrI ACTGG 5 cut(s) 160, 755, 1394, 1819, 2138
BssECI CCNNGG 1 cut(s) 1042
BssMI GATC 8 cut(s) 856, 1027, 1321, 1529, 1993, 2134, 2187, 2293
Bst2UI CCWGG 5 cut(s) 206, 512, 1043, 1827, 2276
Bst4CI ACNGT 7 cut(s) 538, 781, 968, 1088, 1262, 1643, 1715
Bst6I CTCTTC 2 cut(s) 1212, 1413
BstAPI GCANNNNNTGC 1 cut(s) 201
BstAUI TGTACA 1 cut(s) 1130
BstBI TTCGAA 1 cut(s) 2235
BstC8I GCNNGC 1 cut(s) 710
BstF5I GGATG 4 cut(s) 935, 1603, 1911, 2312
BstKTI GATC 8 cut(s) 859, 1030, 1324, 1532, 1996, 2137, 2190, 2296
BstMAI GTCTC 4 cut(s) 153, 527, 663, 1373
BstMBI GATC 8 cut(s) 856, 1027, 1321, 1529, 1993, 2134, 2187, 2293
BstMWI GCNNNNNNNGC 3 cut(s) 201, 1202, 1873
BstNI CCWGG 5 cut(s) 206, 512, 1043, 1827, 2276
BstNSI RCATGY 2 cut(s) 716, 1947
BstSCI CCNGG 5 cut(s) 204, 510, 1041, 1825, 2274
BstSFI CTRYAG 1 cut(s) 2052
BstSLI GKGCMC 1 cut(s) 1393
BstV1I GCAGC 3 cut(s) 112, 1379, 2276
BstV2I GAAGAC 1 cut(s) 2244
BstX2I RGATCY 4 cut(s) 1027, 1529, 1993, 2134
BstYI RGATCY 4 cut(s) 1027, 1529, 1993, 2134
Bsu15I ATCGAT 2 cut(s) 1843, 2186
Bsu36I CCTNAGG 1 cut(s) 1938
BsuRI GGCC 5 cut(s) 518, 1620, 1759, 1971, 2279
BsuTUI ATCGAT 2 cut(s) 1843, 2186
BtrI CACGTC 2 cut(s) 2225, 2242
BtsCI GGATG 4 cut(s) 935, 1603, 1911, 2312
BtsI GCAGTG 1 cut(s) 2280
BtsIMutI CAGTG 9 cut(s) 33, 153, 219, 642, 762, 883, 1084, 1812, 2280
Cac8I GCNNGC 1 cut(s) 710
Cfr13I GGNCC 5 cut(s) 389, 953, 1512, 1970, 2048
ClaI ATCGAT 2 cut(s) 1843, 2186
Csp6I GTAC 9 cut(s) 60, 696, 782, 1050, 1131, 1220, 1229, 1716, 2027
CviAII CATG 8 cut(s) 65, 201, 235, 691, 713, 1115, 1385, 1944
CviQI GTAC 9 cut(s) 60, 696, 782, 1050, 1131, 1220, 1229, 1716, 2027
DpnI GATC 8 cut(s) 858, 1029, 1323, 1531, 1995, 2136, 2189, 2295
DpnII GATC 8 cut(s) 856, 1027, 1321, 1529, 1993, 2134, 2187, 2293
DraIII CACNNNGTG 1 cut(s) 1388
DrdI GACNNNNNNGTC 1 cut(s) 334
DseDI GACNNNNNNGTC 1 cut(s) 334
Eam1104I CTCTTC 2 cut(s) 1212, 1413
EarI CTCTTC 2 cut(s) 1212, 1413
Ecl136II GAGCTC 3 cut(s) 226, 444, 1205
Eco24I GRGCYC 3 cut(s) 228, 446, 1207
Eco31I GGTCTC 1 cut(s) 153
Eco32I GATATC 1 cut(s) 480
Eco47I GGWCC 4 cut(s) 389, 953, 1512, 2048
Eco53kI GAGCTC 3 cut(s) 226, 444, 1205
Eco81I CCTNAGG 1 cut(s) 1938
EcoICRI GAGCTC 3 cut(s) 226, 444, 1205
EcoO109I RGGNCCY 1 cut(s) 1512
EcoRII CCWGG 5 cut(s) 204, 510, 1041, 1825, 2274
EcoRV GATATC 1 cut(s) 480
EcoT22I ATGCAT 1 cut(s) 2204
EcoT38I GRGCYC 3 cut(s) 228, 446, 1207
FaeI CATG 8 cut(s) 68, 204, 238, 694, 716, 1118, 1388, 1947
FalI AAGNNNNNCTT 2 cut(s) 2229, 2261
FaqI GGGAC 4 cut(s) 402, 1525, 1894, 2211
FatI CATG 8 cut(s) 64, 200, 234, 690, 712, 1114, 1384, 1943
FbaI TGATCA 1 cut(s) 2293
Fnu4HI GCNGC 3 cut(s) 101, 1368, 2265
FokI GGATG 4 cut(s) 922, 1590, 1918, 2299
FriOI GRGCYC 3 cut(s) 228, 446, 1207
Fsp4HI GCNGC 3 cut(s) 101, 1368, 2265
FspBI CTAG 6 cut(s) 348, 1161, 1184, 1350, 1701, 1868
GluI GCNGC 3 cut(s) 101, 1368, 2265
GsuI CTGGAG 4 cut(s) 177, 533, 2018, 2193
HaeIII GGCC 5 cut(s) 518, 1620, 1759, 1971, 2279
HapII CCGG 1 cut(s) 818
Hin1II CATG 8 cut(s) 68, 204, 238, 694, 716, 1118, 1388, 1947
HindIII AAGCTT 3 cut(s) 245, 494, 1595
HpaII CCGG 1 cut(s) 818
HphI GGTGA 7 cut(s) 250, 562, 842, 1305, 1478, 1592, 2015
Hpy166II GTNNAC 3 cut(s) 953, 1649, 2011
Hpy188III TCNNGA 7 cut(s) 398, 542, 675, 807, 1739, 1997, 2210
Hpy8I GTNNAC 3 cut(s) 953, 1649, 2011
Hpy99I CGWCG 1 cut(s) 2048
HpyAV CCTTC 8 cut(s) 135, 624, 1044, 1420, 1463, 1476, 1738, 1961
HpyCH4III ACNGT 7 cut(s) 538, 781, 968, 1088, 1262, 1643, 1715
HpyCH4IV ACGT 2 cut(s) 2224, 2241
HpyF10VI GCNNNNNNNGC 3 cut(s) 201, 1202, 1873
HpySE526I ACGT 2 cut(s) 2224, 2241
Hsp92II CATG 8 cut(s) 68, 204, 238, 694, 716, 1118, 1388, 1947
Ksp22I TGATCA 1 cut(s) 2293
Kzo9I GATC 8 cut(s) 856, 1027, 1321, 1529, 1993, 2134, 2187, 2293
LguI GCTCTTC 1 cut(s) 1212
LmnI GCTCC 3 cut(s) 881, 1202, 2106
Lsp1109I GCAGC 3 cut(s) 112, 1379, 2276
LweI GCATC 6 cut(s) 750, 944, 1885, 2105, 2278, 2296
MaeI CTAG 6 cut(s) 348, 1161, 1184, 1350, 1701, 1868
MaeII ACGT 2 cut(s) 2224, 2241
MaeIII GTNAC 7 cut(s) 167, 256, 325, 775, 1082, 1339, 1895
MalI GATC 8 cut(s) 858, 1029, 1323, 1531, 1995, 2136, 2189, 2295
MboI GATC 8 cut(s) 856, 1027, 1321, 1529, 1993, 2134, 2187, 2293
MflI RGATCY 4 cut(s) 1027, 1529, 1993, 2134
MhlI GDGCHC 5 cut(s) 228, 446, 886, 1207, 1393
MlyI GAGTC 3 cut(s) 548, 847, 2174
MmeI TCCRAC 3 cut(s) 531, 1482, 2026
Mph1103I ATGCAT 1 cut(s) 2204
MroXI GAANNNNTTC 2 cut(s) 800, 2076
MseI TTAA 4 cut(s) 252, 633, 701, 1569
MslI CAYNNNNRTG 1 cut(s) 1113
MspA1I CMGCKG 2 cut(s) 103, 1579
MspI CCGG 1 cut(s) 818
MspR9I CCNGG 5 cut(s) 206, 512, 1043, 1827, 2276
Mva1269I GAATGC 1 cut(s) 1102
MvaI CCWGG 5 cut(s) 206, 512, 1043, 1827, 2276
MwoI GCNNNNNNNGC 3 cut(s) 201, 1202, 1873
NdeII GATC 8 cut(s) 856, 1027, 1321, 1529, 1993, 2134, 2187, 2293
NlaIII CATG 8 cut(s) 68, 204, 238, 694, 716, 1118, 1388, 1947
NlaIV GGNNCC 5 cut(s) 390, 1390, 1513, 2108, 2136
NmeAIII GCCGAG 1 cut(s) 81
NmuCI GTSAC 3 cut(s) 256, 325, 1895
NsiI ATGCAT 1 cut(s) 2204
NspI RCATGY 2 cut(s) 716, 1947
NspV TTCGAA 1 cut(s) 2235
PciSI GCTCTTC 1 cut(s) 1212
PctI GAATGC 1 cut(s) 1102
PdmI GAANNNNTTC 2 cut(s) 800, 2076
PfeI GAWTC 7 cut(s) 406, 743, 814, 1583, 1658, 1882, 2036
PkrI GCNGC 3 cut(s) 102, 1369, 2266
PleI GAGTC 3 cut(s) 548, 846, 2173
PpsI GAGTC 3 cut(s) 548, 846, 2173
PpuMI RGGWCCY 1 cut(s) 1512
Psp124BI GAGCTC 3 cut(s) 228, 446, 1207
Psp5II RGGWCCY 1 cut(s) 1512
Psp6I CCWGG 5 cut(s) 204, 510, 1041, 1825, 2274
PspGI CCWGG 5 cut(s) 204, 510, 1041, 1825, 2274
PspN4I GGNNCC 5 cut(s) 390, 1390, 1513, 2108, 2136
PspPI GGNCC 5 cut(s) 389, 953, 1512, 1970, 2048
PspPPI RGGWCCY 1 cut(s) 1512
PsuI RGATCY 4 cut(s) 1027, 1529, 1993, 2134
PvuII CAGCTG 2 cut(s) 103, 1579
RsaI GTAC 9 cut(s) 61, 697, 783, 1051, 1132, 1221, 1230, 1717, 2028
RsaNI GTAC 9 cut(s) 60, 696, 782, 1050, 1131, 1220, 1229, 1716, 2027
RseI CAYNNNNRTG 1 cut(s) 1113
SacI GAGCTC 3 cut(s) 228, 446, 1207
SapI GCTCTTC 1 cut(s) 1212
SaqAI TTAA 4 cut(s) 252, 633, 701, 1569
SatI GCNGC 3 cut(s) 101, 1368, 2265
Sau3AI GATC 8 cut(s) 856, 1027, 1321, 1529, 1993, 2134, 2187, 2293
Sau96I GGNCC 5 cut(s) 389, 953, 1512, 1970, 2048
ScaI AGTACT 3 cut(s) 697, 783, 1221
SchI GAGTC 3 cut(s) 548, 847, 2174
ScrFI CCNGG 5 cut(s) 206, 512, 1043, 1827, 2276
SduI GDGCHC 5 cut(s) 228, 446, 886, 1207, 1393
SfaNI GCATC 6 cut(s) 750, 944, 1885, 2105, 2278, 2296
SfcI CTRYAG 1 cut(s) 2052
SfuI TTCGAA 1 cut(s) 2235
SinI GGWCC 4 cut(s) 389, 953, 1512, 2048
SmiMI CAYNNNNRTG 1 cut(s) 1113
SmlI CTYRAG 5 cut(s) 352, 599, 1362, 1751, 2268
SmoI CTYRAG 5 cut(s) 352, 599, 1362, 1751, 2268
SsiI CCGC 1 cut(s) 1235
SspMI CTAG 6 cut(s) 348, 1161, 1184, 1350, 1701, 1868
SstI GAGCTC 3 cut(s) 228, 446, 1207
StyD4I CCNGG 5 cut(s) 204, 510, 1041, 1825, 2274
TaaI ACNGT 7 cut(s) 538, 781, 968, 1088, 1262, 1643, 1715
TaiI ACGT 2 cut(s) 2227, 2244
TaqI TCGA 5 cut(s) 1402, 1843, 2119, 2186, 2235
TatI WGTACW 6 cut(s) 695, 781, 1130, 1219, 1228, 2026
TfiI GAWTC 7 cut(s) 406, 743, 814, 1583, 1658, 1882, 2036
Tru1I TTAA 4 cut(s) 252, 633, 701, 1569
Tru9I TTAA 4 cut(s) 252, 633, 701, 1569
TscAI CASTG 9 cut(s) 33, 160, 226, 649, 762, 883, 1084, 1819, 2287
TseFI GTSAC 3 cut(s) 256, 325, 1895
TseI GCWGC 3 cut(s) 100, 1367, 2264
Tsp45I GTSAC 3 cut(s) 256, 325, 1895
TspDTI ATGAA 6 cut(s) 189, 398, 442, 1113, 1704, 2103
TspGWI ACGGA 1 cut(s) 2305
TspRI CASTG 9 cut(s) 33, 160, 226, 649, 762, 883, 1084, 1819, 2287
VpaK11BI GGWCC 4 cut(s) 389, 953, 1512, 2048
XapI RAATTY 3 cut(s) 211, 448, 928
XceI RCATGY 2 cut(s) 716, 1947
XmnI GAANNNNTTC 2 cut(s) 800, 2076
XspI CTAG 6 cut(s) 348, 1161, 1184, 1350, 1701, 1868
ZrmI AGTACT 3 cut(s) 697, 783, 1221
Zsp2I ATGCAT 1 cut(s) 2204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.