MD17G1070300.v1.1

S-adenosylmethionine decarboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0008254
Physical Location & Seq
Reverse (-)
5683437 .. 5684610
1174 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1070300.v1.1.491

Sequence Viewer

Length: 1125 bp
ATGGCTGTACCGGTCTCTGCAATTGGATTCGAAGGGTATGAAAAGAGGCTCGAGGTCTCTTTCTTCGAGCCTGGACTGTTCGCTGACCCTAAAGGCATGGGTCTTCGTTCTCTATCGAAAGCTCAAATAGATGAGATTCTGACACCAGCTGAGTGCACCATTGTTTCTTCACTGTCAAATGATGATCTCGACTCTTACGTCCTATCCGAGTCAAGCCTGTTTGTGTACCCTTACAAAGTGATCATCAAAACCTGTGGCACAACGAAATTGCTTCGATCAATCCCTGCCATCCTCAAGTTGGCTGATACCCTCTCTCTAGCTGTAAAATCTGTGAGGTACTCTCGTGGGAGCTTTATCTTTCCTGGTGCTCAGCCCTCTCCTCATCGTAGCTTTTCCGAGGAAGTAGCTGTACTTGACGGCCATTTTAGCAAGCTTGGTTTGGCAAGCAAGGCATATGTTATGGGAAGCCCTGACAAAACTCAGAAATGGCATATTTACTCTGCATCGGCGGAGCTGGCAAGCTTATTGTGGGGTTCACGCCAAACAGGCCCGACCTACACACTGGAGATGTGCATGACCGGTCTAGACAGGAAGAGGGCTTCTGTGTTTTACAAATCCGATGCAAGTTCAGCTGCTGGTATGACCGAAGAATCTGGCATCAGGAAGGTCCTCCCACAATCTGATATATGCGACTTTGAGTTCGAACCTTGCGGTTACTCCATGAACTCAATTGAAGGGAATGCAGTTTCTACGATCCACGTGACACCAGAAGACGGTTTCAGCTACGCTAGCTTTGAAACAGTGGGATATAATTTCAATGATGTGAACTTGACCCTGCTGCTTTACAGGGTTCTGGATTGCTTCAAACCAGCTGAGTTCTCTGTAGCCTTGCACACCACCAGCACCGCAGGCGAAGATCTTGATGCTAAGTGCCCTCTGGACTTAAGGGGATACTGCTGCGGAGGAAGCAGCTATGAGGGTCTAGGCCTGGGTGGTGCAATCATCTACCACAGCTTTGTCAAGGATGATTCTGGTTCGCAGTCTCCAAGGTCGATTCTGAAGTGCTGCTGGAGCGAGGACGAGAAGGACGAGGAAGTTGAAGAGATAGATCTGGCCGAGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00330 ko01100 map00270 map00330 map01100
Pfam Domains
Protein Families
AdoMetDC_leader SAM_decarbox

Protein Analysis

375

Amino Acids

40.78

Weight (kDa)

4.79

Isoelectric Point (pI)

46.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAM_decarbox PF01536 9 - 339 3.5e-115 Adenosylmethionine decarboxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 197
AciI CCGC 4 cut(s) 509, 711, 906, 960
AclWI GGATC 1 cut(s) 748
AcoI YGGCCR 2 cut(s) 418, 1113
AcuI CTGAAG 1 cut(s) 1079
AcvI CACGTG 1 cut(s) 760
AfaI GTAC 4 cut(s) 9, 227, 338, 411
AfiI CCNNNNNNNGG 2 cut(s) 298, 773
AflII CTTAAG 1 cut(s) 943
AgeI ACCGGT 2 cut(s) 10, 578
AgsI TTSAA 5 cut(s) 734, 797, 817, 865, 1100
AjnI CCWGG 3 cut(s) 70, 361, 987
Alw21I GWGCWC 2 cut(s) 158, 370
Alw26I GTCTC 3 cut(s) 19, 61, 1047
Alw44I GTGCAC 1 cut(s) 154
AlwI GGATC 1 cut(s) 748
AlwNI CAGNNNCTG 1 cut(s) 635
Ama87I CYCGRG 1 cut(s) 50
AoxI GGCC 4 cut(s) 418, 547, 985, 1113
ApaLI GTGCAC 1 cut(s) 154
ApeKI GCWGC 5 cut(s) 632, 838, 957, 969, 1065
ArsI GACNNNNNNTTYG 2 cut(s) 683, 715
AsiGI ACCGGT 2 cut(s) 10, 578
AspS9I GGNCC 2 cut(s) 548, 667
AsuII TTCGAA 2 cut(s) 30, 702
AsuNHI GCTAGC 1 cut(s) 788
AvaI CYCGRG 1 cut(s) 50
AvaII GGWCC 1 cut(s) 667
BaeGI GKGCMC 2 cut(s) 158, 935
BaeI ACNNNNGTAYC 2 cut(s) 328, 361
BarI GAAGNNNNNNTAC 2 cut(s) 393, 425
BauI CACGAG 1 cut(s) 342
BbrPI CACGTG 1 cut(s) 760
BbsI GAAGAC 2 cut(s) 95, 777
Bbv12I GWGCWC 2 cut(s) 158, 370
BbvI GCAGC 5 cut(s) 619, 825, 944, 981, 1052
BccI CCATC 1 cut(s) 296
BceAI ACGGC 1 cut(s) 433
BciT130I CCWGG 3 cut(s) 72, 363, 989
BciVI GTATCC 1 cut(s) 944
BclI TGATCA 1 cut(s) 240
BcoDI GTCTC 3 cut(s) 19, 61, 1047
BfaI CTAG 5 cut(s) 317, 584, 789, 983, 1123
BfmI CTRYAG 1 cut(s) 882
BfrI CTTAAG 1 cut(s) 943
BfuI GTATCC 1 cut(s) 944
BglI GCCNNNNNGGC 1 cut(s) 546
BglII AGATCT 3 cut(s) 916, 1108, 1119
BisI GCNGC 5 cut(s) 633, 839, 958, 970, 1066
BlpI GCTNAGC 1 cut(s) 369
BlsI GCNGC 5 cut(s) 634, 840, 959, 971, 1067
Bme1390I CCNGG 3 cut(s) 72, 363, 989
Bme18I GGWCC 1 cut(s) 667
BmeT110I CYCGRG 1 cut(s) 50
BmgT120I GGNCC 2 cut(s) 548, 667
BmrFI CCNGG 3 cut(s) 72, 363, 989
BmsI GCATC 4 cut(s) 512, 610, 666, 913
BmtI GCTAGC 1 cut(s) 792
BpiI GAAGAC 2 cut(s) 95, 777
BplI GAGNNNNNCTC 2 cut(s) 325, 357
BpmI CTGGAG 2 cut(s) 584, 1090
Bpu1102I GCTNAGC 1 cut(s) 369
Bpu14I TTCGAA 2 cut(s) 30, 702
BpuEI CTTGAG 1 cut(s) 278
BsaAI YACGTR 1 cut(s) 760
BsaI GGTCTC 2 cut(s) 19, 61
BsaJI CCNNGG 3 cut(s) 396, 988, 1046
BsaWI WCCGGW 2 cut(s) 10, 578
Bsc4I CCNNNNNNNGG 2 cut(s) 298, 773
Bse118I RCCGGY 2 cut(s) 10, 578
Bse1I ACTGG 1 cut(s) 567
BseBI CCWGG 3 cut(s) 72, 363, 989
BseDI CCNNGG 3 cut(s) 396, 988, 1046
BseGI GGATG 2 cut(s) 288, 1030
BseLI CCNNNNNNNGG 2 cut(s) 298, 773
BseMII CTCAG 4 cut(s) 141, 383, 494, 864
BseNI ACTGG 1 cut(s) 567
BseRI GAGGAG 1 cut(s) 369
BseSI GKGCMC 2 cut(s) 158, 935
BseXI GCAGC 5 cut(s) 619, 825, 944, 981, 1052
BshFI GGCC 4 cut(s) 420, 549, 987, 1115
BshTI ACCGGT 2 cut(s) 10, 578
BsiHKAI GWGCWC 2 cut(s) 158, 370
BsiHKCI CYCGRG 1 cut(s) 50
BsiSI CCGG 2 cut(s) 11, 579
BslI CCNNNNNNNGG 2 cut(s) 298, 773
BsmAI GTCTC 3 cut(s) 19, 61, 1047
BsmI GAATGC 1 cut(s) 745
BsnI GGCC 4 cut(s) 420, 549, 987, 1115
Bso31I GGTCTC 2 cut(s) 19, 61
BsoBI CYCGRG 1 cut(s) 50
Bsp119I TTCGAA 2 cut(s) 30, 702
Bsp1286I GDGCHC 3 cut(s) 158, 370, 935
Bsp143I GATC 7 cut(s) 184, 240, 275, 753, 916, 1108, 1119
Bsp1720I GCTNAGC 1 cut(s) 369
BspACI CCGC 4 cut(s) 509, 711, 906, 960
BspANI GGCC 4 cut(s) 420, 549, 987, 1115
BspCNI CTCAG 4 cut(s) 142, 382, 493, 865
BspOI GCTAGC 1 cut(s) 792
BspPI GGATC 1 cut(s) 748
BspT104I TTCGAA 2 cut(s) 30, 702
BspTI CTTAAG 1 cut(s) 943
BspTNI GGTCTC 2 cut(s) 19, 61
BsrFI RCCGGY 2 cut(s) 10, 578
BsrI ACTGG 1 cut(s) 567
BssAI RCCGGY 2 cut(s) 10, 578
BssECI CCNNGG 3 cut(s) 396, 988, 1046
BssMI GATC 7 cut(s) 184, 240, 275, 753, 916, 1108, 1119
BssSI CACGAG 1 cut(s) 342
BssT1I CCWWGG 1 cut(s) 1046
Bst2BI CACGAG 1 cut(s) 342
Bst2UI CCWGG 3 cut(s) 72, 363, 989
Bst4CI ACNGT 4 cut(s) 78, 174, 776, 802
Bst6I CTCTTC 2 cut(s) 587, 1095
BstAFI CTTAAG 1 cut(s) 943
BstBAI YACGTR 1 cut(s) 760
BstBI TTCGAA 2 cut(s) 30, 702
BstC8I GCNNGC 6 cut(s) 431, 445, 516, 520, 790, 910
BstDEI CTNAG 5 cut(s) 150, 369, 480, 873, 927
BstF5I GGATG 2 cut(s) 288, 1030
BstKTI GATC 7 cut(s) 187, 243, 278, 756, 919, 1111, 1122
BstMAI GTCTC 3 cut(s) 19, 61, 1047
BstMBI GATC 7 cut(s) 184, 240, 275, 753, 916, 1108, 1119
BstMWI GCNNNNNNNGC 9 cut(s) 426, 449, 515, 546, 629, 789, 909, 966, 1071
BstNI CCWGG 3 cut(s) 72, 363, 989
BstSCI CCNGG 3 cut(s) 70, 361, 987
BstSFI CTRYAG 1 cut(s) 882
BstSLI GKGCMC 2 cut(s) 158, 935
BstV1I GCAGC 5 cut(s) 619, 825, 944, 981, 1052
BstV2I GAAGAC 2 cut(s) 95, 777
BstX2I RGATCY 3 cut(s) 916, 1108, 1119
BstYI RGATCY 3 cut(s) 916, 1108, 1119
BsuI GTATCC 1 cut(s) 944
BsuRI GGCC 4 cut(s) 420, 549, 987, 1115
BtsCI GGATG 2 cut(s) 288, 1030
BtsIMutI CAGTG 3 cut(s) 170, 560, 807
Cac8I GCNNGC 6 cut(s) 431, 445, 516, 520, 790, 910
CaiI CAGNNNCTG 1 cut(s) 635
Cfr10I RCCGGY 2 cut(s) 10, 578
Cfr13I GGNCC 2 cut(s) 548, 667
Csp6I GTAC 4 cut(s) 8, 226, 337, 410
CspAI ACCGGT 2 cut(s) 10, 578
CspCI CAANNNNNGTGG 2 cut(s) 235, 270
CviAII CATG 3 cut(s) 97, 574, 721
CviQI GTAC 4 cut(s) 8, 226, 337, 410
DdeI CTNAG 5 cut(s) 150, 369, 480, 873, 927
DpnI GATC 7 cut(s) 186, 242, 277, 755, 918, 1110, 1121
DpnII GATC 7 cut(s) 184, 240, 275, 753, 916, 1108, 1119
DrdI GACNNNNNNGTC 1 cut(s) 197
DseDI GACNNNNNNGTC 1 cut(s) 197
EaeI YGGCCR 2 cut(s) 418, 1113
Eam1104I CTCTTC 2 cut(s) 587, 1095
EarI CTCTTC 2 cut(s) 587, 1095
EciI GGCGGA 1 cut(s) 524
Eco130I CCWWGG 1 cut(s) 1046
Eco147I AGGCCT 1 cut(s) 987
Eco31I GGTCTC 2 cut(s) 19, 61
Eco47I GGWCC 1 cut(s) 667
Eco57I CTGAAG 1 cut(s) 1079
Eco72I CACGTG 1 cut(s) 760
Eco88I CYCGRG 1 cut(s) 50
EcoO109I RGGNCCY 1 cut(s) 667
EcoRII CCWGG 3 cut(s) 70, 361, 987
EcoT14I CCWWGG 1 cut(s) 1046
ErhI CCWWGG 1 cut(s) 1046
FaeI CATG 3 cut(s) 100, 577, 724
FatI CATG 3 cut(s) 96, 573, 720
FauNDI CATATG 1 cut(s) 454
FbaI TGATCA 1 cut(s) 240
Fnu4HI GCNGC 5 cut(s) 633, 839, 958, 970, 1066
FokI GGATG 2 cut(s) 275, 1037
Fsp4HI GCNGC 5 cut(s) 633, 839, 958, 970, 1066
FspBI CTAG 5 cut(s) 317, 584, 789, 983, 1123
GluI GCNGC 5 cut(s) 633, 839, 958, 970, 1066
GsuI CTGGAG 2 cut(s) 584, 1090
HaeIII GGCC 4 cut(s) 420, 549, 987, 1115
HapII CCGG 2 cut(s) 11, 579
Hin1II CATG 3 cut(s) 100, 577, 724
HindIII AAGCTT 2 cut(s) 431, 520
HinfI GANTC 7 cut(s) 27, 136, 191, 209, 650, 1028, 1054
HpaII CCGG 2 cut(s) 11, 579
Hpy166II GTNNAC 4 cut(s) 156, 226, 536, 826
Hpy188I TCNGA 7 cut(s) 141, 208, 397, 483, 619, 682, 1059
Hpy188III TCNNGA 6 cut(s) 188, 584, 661, 854, 920, 938
Hpy8I GTNNAC 4 cut(s) 156, 226, 536, 826
HpyAV CCTTC 4 cut(s) 26, 658, 728, 1078
HpyCH4III ACNGT 4 cut(s) 78, 174, 776, 802
HpyCH4IV ACGT 2 cut(s) 198, 759
HpyCH4V TGCA 8 cut(s) 20, 156, 503, 573, 623, 743, 892, 998
HpyF10VI GCNNNNNNNGC 9 cut(s) 426, 449, 515, 546, 629, 789, 909, 966, 1071
HpyF3I CTNAG 5 cut(s) 150, 369, 480, 873, 927
HpySE526I ACGT 2 cut(s) 198, 759
Hsp92II CATG 3 cut(s) 100, 577, 724
Ksp22I TGATCA 1 cut(s) 240
Kzo9I GATC 7 cut(s) 184, 240, 275, 753, 916, 1108, 1119
LmnI GCTCC 3 cut(s) 348, 511, 1071
Lsp1109I GCAGC 5 cut(s) 619, 825, 944, 981, 1052
LweI GCATC 4 cut(s) 512, 610, 666, 913
MaeI CTAG 5 cut(s) 317, 584, 789, 983, 1123
MaeII ACGT 2 cut(s) 198, 759
MaeIII GTNAC 2 cut(s) 713, 760
MalI GATC 7 cut(s) 186, 242, 277, 755, 918, 1110, 1121
MboI GATC 7 cut(s) 184, 240, 275, 753, 916, 1108, 1119
MboII GAAGA 8 cut(s) 55, 95, 159, 604, 659, 782, 926, 1112
MfeI CAATTG 2 cut(s) 21, 729
MflI RGATCY 3 cut(s) 916, 1108, 1119
MhlI GDGCHC 3 cut(s) 158, 370, 935
MluCI AATT 4 cut(s) 21, 266, 729, 811
MlyI GAGTC 2 cut(s) 185, 218
MseI TTAA 1 cut(s) 944
MspA1I CMGCKG 3 cut(s) 149, 632, 872
MspCI CTTAAG 1 cut(s) 943
MspI CCGG 2 cut(s) 11, 579
MspR9I CCNGG 3 cut(s) 72, 363, 989
MunI CAATTG 2 cut(s) 21, 729
Mva1269I GAATGC 1 cut(s) 745
MvaI CCWGG 3 cut(s) 72, 363, 989
MwoI GCNNNNNNNGC 9 cut(s) 426, 449, 515, 546, 629, 789, 909, 966, 1071
NdeI CATATG 1 cut(s) 454
NdeII GATC 7 cut(s) 184, 240, 275, 753, 916, 1108, 1119
NheI GCTAGC 1 cut(s) 788
NlaIII CATG 3 cut(s) 100, 577, 724
NmuCI GTSAC 1 cut(s) 760
NspV TTCGAA 2 cut(s) 30, 702
PaeR7I CTCGAG 1 cut(s) 50
PceI AGGCCT 1 cut(s) 987
PcsI WCGNNNNNNNCGW 3 cut(s) 195, 204, 1086
PctI GAATGC 1 cut(s) 745
PfeI GAWTC 5 cut(s) 27, 136, 650, 1028, 1054
PinAI ACCGGT 2 cut(s) 10, 578
PkrI GCNGC 5 cut(s) 634, 840, 959, 971, 1067
PleI GAGTC 2 cut(s) 185, 217
PmaCI CACGTG 1 cut(s) 760
PmlI CACGTG 1 cut(s) 760
PpsI GAGTC 2 cut(s) 185, 217
Ppu21I YACGTR 1 cut(s) 760
PpuMI RGGWCCY 1 cut(s) 667
Psp5II RGGWCCY 1 cut(s) 667
Psp6I CCWGG 3 cut(s) 70, 361, 987
PspCI CACGTG 1 cut(s) 760
PspGI CCWGG 3 cut(s) 70, 361, 987
PspPI GGNCC 2 cut(s) 548, 667
PspPPI RGGWCCY 1 cut(s) 667
PspXI VCTCGAGB 1 cut(s) 50
PstNI CAGNNNCTG 1 cut(s) 635
PsuI RGATCY 3 cut(s) 916, 1108, 1119
PvuII CAGCTG 3 cut(s) 149, 632, 872
RsaI GTAC 4 cut(s) 9, 227, 338, 411
RsaNI GTAC 4 cut(s) 8, 226, 337, 410
SaqAI TTAA 1 cut(s) 944
SatI GCNGC 5 cut(s) 633, 839, 958, 970, 1066
Sau3AI GATC 7 cut(s) 184, 240, 275, 753, 916, 1108, 1119
Sau96I GGNCC 2 cut(s) 548, 667
SchI GAGTC 2 cut(s) 185, 218
ScrFI CCNGG 3 cut(s) 72, 363, 989
SduI GDGCHC 3 cut(s) 158, 370, 935
SfaNI GCATC 4 cut(s) 512, 610, 666, 913
SfcI CTRYAG 1 cut(s) 882
Sfr274I CTCGAG 1 cut(s) 50
SfuI TTCGAA 2 cut(s) 30, 702
SinI GGWCC 1 cut(s) 667
SlaI CTCGAG 1 cut(s) 50
SmlI CTYRAG 3 cut(s) 50, 293, 943
SmoI CTYRAG 3 cut(s) 50, 293, 943
Sse9I AATT 4 cut(s) 21, 266, 729, 811
SseBI AGGCCT 1 cut(s) 987
SsiI CCGC 4 cut(s) 509, 711, 906, 960
SspMI CTAG 5 cut(s) 317, 584, 789, 983, 1123
StuI AGGCCT 1 cut(s) 987
StyD4I CCNGG 3 cut(s) 70, 361, 987
StyI CCWWGG 1 cut(s) 1046
TaaI ACNGT 4 cut(s) 78, 174, 776, 802
TaiI ACGT 2 cut(s) 201, 762
TaqI TCGA 8 cut(s) 30, 51, 66, 116, 189, 274, 702, 1052
TaqII GACCGA 1 cut(s) 659
TasI AATT 4 cut(s) 21, 266, 729, 811
TatI WGTACW 1 cut(s) 409
TfiI GAWTC 5 cut(s) 27, 136, 650, 1028, 1054
Tru1I TTAA 1 cut(s) 944
Tru9I TTAA 1 cut(s) 944
TscAI CASTG 3 cut(s) 177, 567, 807
TseFI GTSAC 1 cut(s) 760
TseI GCWGC 5 cut(s) 632, 838, 957, 969, 1065
Tsp45I GTSAC 1 cut(s) 760
TspDTI ATGAA 2 cut(s) 54, 737
TspRI CASTG 3 cut(s) 177, 567, 807
Vha464I CTTAAG 1 cut(s) 943
VneI GTGCAC 1 cut(s) 154
VpaK11BI GGWCC 1 cut(s) 667
XbaI TCTAGA 1 cut(s) 583
XcmI CCANNNNNNNNNTGG 1 cut(s) 295
XhoI CTCGAG 1 cut(s) 50
XspI CTAG 5 cut(s) 317, 584, 789, 983, 1123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.