MD17G1141200.v1.1

Belongs to the glycosyltransferase 8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0004442
Physical Location & Seq
Forward (+)
12677552 .. 12689049
11498 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1141200.v1.1.491

Sequence Viewer

Length: 1581 bp
ATGAAATTTTATATATCCACGACGGGGATAAAGAGGCTGACGATATCCAGCGCGGGCACCGGGAAGGGGTCTCCCGGGTCTCCGGCGACTCGGAGGATTTCCTGCCGGAGGGTCATGCCGTTGTTGCTGGTTCTCGCACTAGTTTTGCCCTTTCTTTTCGTCAGAATTGCGGTCATTGTCCTTGATTCTTCCACTGCTTGCTCTTCCACTCTCGACTGCGTAGGTTGGAGGATTTTCAGCGGGGGTGACGAATCTACACTCAGAGAGGAGTTAGCAAGAGCACTGCTAGAAGCAAAGGATGGTAACTTAAATAATAATGAGCAAGGGATCGAGTCGTTTAACCAACTGGTGCAGGAGATGACGTTGAAGCAACAAGATGTCAAAGCCTTCGCCTTCAAAACAAAGGCCATGCTGTCAAGAATGGAACACAAGGTTCAGTCAGCCAGACAGCGGGAATCGGTTTACTGGCACTTAGCCTCCCATGGTGTGCCCAAGAGCATACATTGTCTTTGCCTCAAATTAGCAGAAGAATATGCTGTAAATGCAATAGCTCGATCTCATTTACCTCCACCTGAATATGTTTCTCGACTTTCTGATCCCTCCTTTCACCACCTAGTTCTCCTAACTGACAATGTGCTCGCAGCTTCTGTTGTTATCTCCTCTACAGTCCAAAAATCAGCCAAGCCCGAAAAATTGATTTTTCACATAGTGACTGACAAGAAAACTTATACTCCAATGCATGCCTGGTTTGCAATTAATTCTTTTCAATCAGCAGTGGTGGAAGTTAAAGGGCTACACCAGTACGACTGGTCTCAGGAGGTAAATGTTGGAGTCAAGGAGATGCTAGAGATCCATCGCCAGATTTGGAGACGTTACTACAGAAATTTGAAAGAAGATGATTTTGAATCCGATGGAAAACATAAAAGATTCTTGGAAGCTCTGAGTCCCAGCTGCCTTTCCCTTATGAACCTTCTTCGAATCTATATCCCCGAGCTTTTTCCAGATCTCAACAAGATAGTGTTCTTGGACGATGATATCGTTGTACAACATGACTTATCATCTTTGTGGGAACTGGATCTCAATGGAAATGTTGTTGGTGCAGTTGTCAATTCATGGTGCGGTGACAACTGTTGCCCAGGAAGAACTTACAGTGACTACTTCAATTTTTCACACCCTATCATTTCATCCAACTTCGATCATGATCGTTGTGCGTGGCTGTATGGCATGAATGTCTTTGATCTTGAAGCTTGGCGGCAGACCAATATTACAGAAACGTACCATCAATGGTTAAAACTTAACCAGAAGTCTGGGCTGACGTTGTGGCATCCAGGAATAGTTCCACCCGCGTTGATTGCATTTGAGGGTCATGTGCATCCTATTGACCCATCATGTCATGTGGCTGGGCTAGGTTCTCGATCTCCAGAGGTTCCCGAAGATATATTGGAAGCTGCTTCGGTCATCCATTTCAGTGGCCCAGCAAAGCCATGGCTCGAGATTGGGTGTCCAGAGGTAAGAGGCTTGTGGAACAAGCATGTAAACAGTTCAAACAAGTTTGTTAGGCAATGTAGAATCATGGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008194 GO:0016740 GO:0016757 GO:0016758 GO:0047262
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_transf_8

Protein Analysis

527

Amino Acids

59.38

Weight (kDa)

7.07

Isoelectric Point (pI)

50.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_8 PF01501 169 - 499 8.2e-65 Glycosyl transferase family 8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 56
AccII CGCG 2 cut(s) 53, 1346
AciI CCGC 7 cut(s) 53, 170, 240, 451, 1119, 1252, 1344
AclWI GGATC 4 cut(s) 335, 590, 844, 1083
AcsI RAATTY 2 cut(s) 5, 883
AdeI CACNNNGTG 1 cut(s) 709
AfaI GTAC 3 cut(s) 803, 1044, 1277
AfiI CCNNNNNNNGG 4 cut(s) 24, 66, 108, 450
AgsI TTSAA 8 cut(s) 367, 397, 767, 889, 905, 1162, 1244, 1545
AhlI ACTAGT 1 cut(s) 139
AjnI CCWGG 3 cut(s) 743, 1135, 1327
AluBI AGCT 7 cut(s) 551, 644, 938, 951, 994, 1247, 1448
AluI AGCT 7 cut(s) 551, 644, 938, 951, 994, 1247, 1448
Alw21I GWGCWC 2 cut(s) 283, 639
Alw26I GTCTC 4 cut(s) 75, 84, 816, 862
AlwI GGATC 4 cut(s) 335, 590, 844, 1083
AlwNI CAGNNNCTG 1 cut(s) 647
Ama87I CYCGRG 3 cut(s) 74, 989, 1490
AoxI GGCC 2 cut(s) 405, 1471
ApeKI GCWGC 3 cut(s) 641, 951, 1448
ApoI RAATTY 2 cut(s) 5, 883
AseI ATTAAT 1 cut(s) 756
AspLEI GCGC 1 cut(s) 53
AspS9I GGNCC 1 cut(s) 1472
AsuC2I CCSGG 3 cut(s) 61, 75, 76
AsuHPI GGTGA 3 cut(s) 257, 599, 1133
AsuII TTCGAA 1 cut(s) 976
AvaI CYCGRG 3 cut(s) 74, 989, 1490
BaeGI GKGCMC 2 cut(s) 59, 492
BanI GGYRCC 1 cut(s) 56
Bbv12I GWGCWC 2 cut(s) 283, 639
BbvI GCAGC 3 cut(s) 653, 938, 1435
BccI CCATC 5 cut(s) 293, 861, 905, 1287, 1393
BceAI ACGGC 1 cut(s) 103
BciT130I CCWGG 3 cut(s) 745, 1137, 1329
BcnI CCSGG 3 cut(s) 61, 75, 76
BcoDI GTCTC 4 cut(s) 75, 84, 816, 862
BcuI ACTAGT 1 cut(s) 139
BfaI CTAG 5 cut(s) 140, 287, 614, 845, 1406
BfmI CTRYAG 2 cut(s) 663, 877
BglII AGATCT 1 cut(s) 1003
BisI GCNGC 4 cut(s) 642, 952, 1253, 1449
BlsI GCNGC 4 cut(s) 643, 953, 1254, 1450
Bme1390I CCNGG 6 cut(s) 61, 75, 76, 745, 1137, 1329
BmeT110I CYCGRG 3 cut(s) 74, 989, 1490
BmgT120I GGNCC 1 cut(s) 1472
BmiI GGNNCC 2 cut(s) 58, 1428
BmrFI CCNGG 6 cut(s) 61, 75, 76, 745, 1137, 1329
BmsI GCATC 3 cut(s) 831, 1333, 1381
BpmI CTGGAG 1 cut(s) 1404
Bpu14I TTCGAA 1 cut(s) 976
BpuMI CCSGG 3 cut(s) 61, 75, 76
BsaBI GATNNNNATC 1 cut(s) 1200
BsaI GGTCTC 3 cut(s) 75, 84, 816
BsaJI CCNNGG 4 cut(s) 74, 481, 1135, 1484
BsaXI ACNNNNNCTCC 6 cut(s) 347, 377, 461, 491, 715, 745
Bsc4I CCNNNNNNNGG 4 cut(s) 24, 66, 108, 450
Bse1I ACTGG 5 cut(s) 351, 470, 799, 812, 1077
Bse3DI GCAATG 1 cut(s) 1568
Bse8I GATNNNNATC 1 cut(s) 1200
BseBI CCWGG 3 cut(s) 745, 1137, 1329
BseDI CCNNGG 4 cut(s) 74, 481, 1135, 1484
BseGI GGATG 5 cut(s) 304, 1184, 1324, 1372, 1458
BseJI GATNNNNATC 1 cut(s) 1200
BseLI CCNNNNNNNGG 4 cut(s) 24, 66, 108, 450
BseMI GCAATG 1 cut(s) 1568
BseMII CTCAG 3 cut(s) 274, 827, 932
BseNI ACTGG 5 cut(s) 351, 470, 799, 812, 1077
BseRI GAGGAG 2 cut(s) 281, 649
BseSI GKGCMC 2 cut(s) 59, 492
BseXI GCAGC 3 cut(s) 653, 938, 1435
BseYI CCCAGC 3 cut(s) 947, 1400, 1474
BsgI GTGCAG 2 cut(s) 371, 1119
Bsh1236I CGCG 2 cut(s) 53, 1346
BshFI GGCC 2 cut(s) 407, 1473
BshNI GGYRCC 1 cut(s) 56
BsiHKAI GWGCWC 2 cut(s) 283, 639
BsiHKCI CYCGRG 3 cut(s) 74, 989, 1490
BsiSI CCGG 4 cut(s) 60, 75, 83, 106
BslFI GGGAC 1 cut(s) 930
BslI CCNNNNNNNGG 4 cut(s) 24, 66, 108, 450
BsmAI GTCTC 4 cut(s) 75, 84, 816, 862
BsmBI CGTCTC 1 cut(s) 862
BsmFI GGGAC 1 cut(s) 930
BsnI GGCC 2 cut(s) 407, 1473
Bso31I GGTCTC 3 cut(s) 75, 84, 816
BsoBI CYCGRG 3 cut(s) 74, 989, 1490
Bsp119I TTCGAA 1 cut(s) 976
Bsp1286I GDGCHC 4 cut(s) 59, 283, 492, 639
Bsp1407I TGTACA 1 cut(s) 1042
Bsp19I CCATGG 2 cut(s) 481, 1484
BspACI CCGC 7 cut(s) 53, 170, 240, 451, 1119, 1252, 1344
BspANI GGCC 2 cut(s) 407, 1473
BspCNI CTCAG 3 cut(s) 273, 826, 933
BspFNI CGCG 2 cut(s) 53, 1346
BspHI TCATGA 1 cut(s) 1198
BspLI GGNNCC 2 cut(s) 58, 1428
BspPI GGATC 4 cut(s) 335, 590, 844, 1083
BspQI GCTCTTC 1 cut(s) 208
BspT104I TTCGAA 1 cut(s) 976
BspT107I GGYRCC 1 cut(s) 56
BspTNI GGTCTC 3 cut(s) 75, 84, 816
BsrDI GCAATG 1 cut(s) 1568
BsrGI TGTACA 1 cut(s) 1042
BsrI ACTGG 5 cut(s) 351, 470, 799, 812, 1077
BssECI CCNNGG 4 cut(s) 74, 481, 1135, 1484
BssT1I CCWWGG 2 cut(s) 481, 1484
Bst2UI CCWGG 3 cut(s) 745, 1137, 1329
Bst4CI ACNGT 4 cut(s) 667, 1130, 1151, 1541
Bst6I CTCTTC 1 cut(s) 208
BstAUI TGTACA 1 cut(s) 1042
BstBI TTCGAA 1 cut(s) 976
BstC8I GCNNGC 4 cut(s) 55, 199, 639, 741
BstDEI CTNAG 4 cut(s) 260, 472, 813, 941
BstDSI CCRYGG 2 cut(s) 481, 1484
BstENI CCTNNNNNAGG 1 cut(s) 106
BstF5I GGATG 5 cut(s) 304, 1184, 1324, 1372, 1458
BstFNI CGCG 2 cut(s) 53, 1346
BstHHI GCGC 1 cut(s) 53
BstMAI GTCTC 4 cut(s) 75, 84, 816, 862
BstMWI GCNNNNNNNGC 4 cut(s) 124, 542, 749, 1352
BstNI CCWGG 3 cut(s) 745, 1137, 1329
BstNSI RCATGY 2 cut(s) 743, 1535
BstSCI CCNGG 6 cut(s) 59, 73, 74, 743, 1135, 1327
BstSFI CTRYAG 2 cut(s) 663, 877
BstSLI GKGCMC 2 cut(s) 59, 492
BstUI CGCG 2 cut(s) 53, 1346
BstV1I GCAGC 3 cut(s) 653, 938, 1435
BstX2I RGATCY 3 cut(s) 849, 1003, 1075
BstXI CCANNNNNNTGG 2 cut(s) 1307, 1469
BstYI RGATCY 3 cut(s) 849, 1003, 1075
BsuRI GGCC 2 cut(s) 407, 1473
BtgI CCRYGG 2 cut(s) 481, 1484
BtgZI GCGATG 1 cut(s) 839
BtsCI GGATG 5 cut(s) 304, 1184, 1324, 1372, 1458
BtsI GCAGTG 3 cut(s) 192, 281, 780
BtsIMutI CAGTG 5 cut(s) 192, 281, 780, 1156, 1474
Cac8I GCNNGC 4 cut(s) 55, 199, 639, 741
CaiI CAGNNNCTG 1 cut(s) 647
CciI TCATGA 1 cut(s) 1198
CfoI GCGC 1 cut(s) 53
Cfr13I GGNCC 1 cut(s) 1472
Cfr9I CCCGGG 1 cut(s) 74
Csp6I GTAC 3 cut(s) 802, 1043, 1276
CspCI CAANNNNNGTGG 2 cut(s) 1329, 1364
CviQI GTAC 3 cut(s) 802, 1043, 1276
DdeI CTNAG 4 cut(s) 260, 472, 813, 941
DraIII CACNNNGTG 1 cut(s) 709
Eam1104I CTCTTC 1 cut(s) 208
EarI CTCTTC 1 cut(s) 208
Eco130I CCWWGG 2 cut(s) 481, 1484
Eco31I GGTCTC 3 cut(s) 75, 84, 816
Eco32I GATATC 2 cut(s) 45, 1036
Eco88I CYCGRG 3 cut(s) 74, 989, 1490
EcoNI CCTNNNNNAGG 1 cut(s) 106
EcoRII CCWGG 3 cut(s) 743, 1135, 1327
EcoRV GATATC 2 cut(s) 45, 1036
EcoT14I CCWWGG 2 cut(s) 481, 1484
EcoT22I ATGCAT 1 cut(s) 741
ErhI CCWWGG 2 cut(s) 481, 1484
Esp3I CGTCTC 1 cut(s) 862
FaqI GGGAC 1 cut(s) 930
FauI CCCGC 4 cut(s) 46, 233, 444, 1351
Fnu4HI GCNGC 4 cut(s) 642, 952, 1253, 1449
FokI GGATG 5 cut(s) 311, 1171, 1311, 1359, 1445
Fsp4HI GCNGC 4 cut(s) 642, 952, 1253, 1449
FspBI CTAG 5 cut(s) 140, 287, 614, 845, 1406
GlaI GCGC 1 cut(s) 52
GluI GCNGC 4 cut(s) 642, 952, 1253, 1449
GsaI CCCAGC 3 cut(s) 951, 1404, 1478
GsuI CTGGAG 1 cut(s) 1404
HaeIII GGCC 2 cut(s) 407, 1473
HapII CCGG 4 cut(s) 60, 75, 83, 106
HhaI GCGC 1 cut(s) 53
Hin6I GCGC 1 cut(s) 51
HinP1I GCGC 1 cut(s) 51
HindIII AAGCTT 1 cut(s) 1245
HpaII CCGG 4 cut(s) 60, 75, 83, 106
HphI GGTGA 3 cut(s) 257, 599, 1133
Hpy166II GTNNAC 2 cut(s) 463, 1537
Hpy188I TCNGA 6 cut(s) 93, 164, 263, 595, 910, 942
Hpy8I GTNNAC 2 cut(s) 463, 1537
Hpy99I CGWCG 1 cut(s) 25
HpyAV CCTTC 4 cut(s) 58, 397, 403, 980
HpyCH4III ACNGT 4 cut(s) 667, 1130, 1151, 1541
HpyCH4IV ACGT 4 cut(s) 362, 871, 1274, 1316
HpyCH4V TGCA 7 cut(s) 352, 545, 739, 752, 1100, 1355, 1372
HpyF10VI GCNNNNNNNGC 4 cut(s) 124, 542, 749, 1352
HpyF3I CTNAG 4 cut(s) 260, 472, 813, 941
HpySE526I ACGT 4 cut(s) 362, 871, 1274, 1316
HspAI GCGC 1 cut(s) 51
LguI GCTCTTC 1 cut(s) 208
Lsp1109I GCAGC 3 cut(s) 653, 938, 1435
LweI GCATC 3 cut(s) 831, 1333, 1381
MaeI CTAG 5 cut(s) 140, 287, 614, 845, 1406
MaeII ACGT 4 cut(s) 362, 871, 1274, 1316
MaeIII GTNAC 6 cut(s) 245, 302, 709, 872, 1121, 1151
MboII GAAGA 7 cut(s) 180, 195, 539, 905, 965, 1152, 1445
MflI RGATCY 3 cut(s) 849, 1003, 1075
MhlI GDGCHC 4 cut(s) 59, 283, 492, 639
MluCI AATT 9 cut(s) 5, 165, 518, 692, 753, 757, 883, 1108, 1162
MlyI GAGTC 4 cut(s) 82, 341, 840, 952
MmeI TCCRAC 3 cut(s) 206, 808, 1212
Mph1103I ATGCAT 1 cut(s) 741
MseI TTAA 6 cut(s) 308, 339, 756, 786, 1289, 1296
MslI CAYNNNNRTG 2 cut(s) 1063, 1467
MspA1I CMGCKG 3 cut(s) 240, 451, 951
MspI CCGG 4 cut(s) 60, 75, 83, 106
MspR9I CCNGG 6 cut(s) 61, 75, 76, 745, 1137, 1329
MvaI CCWGG 3 cut(s) 745, 1137, 1329
MvnI CGCG 2 cut(s) 53, 1346
MwoI GCNNNNNNNGC 4 cut(s) 124, 542, 749, 1352
NciI CCSGG 3 cut(s) 61, 75, 76
NcoI CCATGG 2 cut(s) 481, 1484
NlaIV GGNNCC 2 cut(s) 58, 1428
NmuCI GTSAC 4 cut(s) 245, 709, 1121, 1151
NsiI ATGCAT 1 cut(s) 741
NspI RCATGY 2 cut(s) 743, 1535
NspV TTCGAA 1 cut(s) 976
PaeI GCATGC 1 cut(s) 743
PaeR7I CTCGAG 1 cut(s) 1490
PagI TCATGA 1 cut(s) 1198
PciSI GCTCTTC 1 cut(s) 208
PcsI WCGNNNNNNNCGW 1 cut(s) 1035
PfeI GAWTC 7 cut(s) 185, 251, 455, 905, 927, 978, 1569
PfoI TCCNGGA 1 cut(s) 1327
PkrI GCNGC 4 cut(s) 643, 953, 1254, 1450
PleI GAGTC 4 cut(s) 82, 340, 839, 951
PpsI GAGTC 4 cut(s) 82, 340, 839, 951
PshBI ATTAAT 1 cut(s) 756
Psp6I CCWGG 3 cut(s) 743, 1135, 1327
PspFI CCCAGC 3 cut(s) 947, 1400, 1474
PspGI CCWGG 3 cut(s) 743, 1135, 1327
PspN4I GGNNCC 2 cut(s) 58, 1428
PspPI GGNCC 1 cut(s) 1472
PstNI CAGNNNCTG 1 cut(s) 647
PsuI RGATCY 3 cut(s) 849, 1003, 1075
PvuII CAGCTG 1 cut(s) 951
RsaI GTAC 3 cut(s) 803, 1044, 1277
RsaNI GTAC 3 cut(s) 802, 1043, 1276
RseI CAYNNNNRTG 2 cut(s) 1063, 1467
SapI GCTCTTC 1 cut(s) 208
SaqAI TTAA 6 cut(s) 308, 339, 756, 786, 1289, 1296
SatI GCNGC 4 cut(s) 642, 952, 1253, 1449
Sau96I GGNCC 1 cut(s) 1472
SchI GAGTC 4 cut(s) 82, 341, 840, 952
ScrFI CCNGG 6 cut(s) 61, 75, 76, 745, 1137, 1329
SduI GDGCHC 4 cut(s) 59, 283, 492, 639
SfaNI GCATC 3 cut(s) 831, 1333, 1381
SfcI CTRYAG 2 cut(s) 663, 877
Sfr274I CTCGAG 1 cut(s) 1490
SfuI TTCGAA 1 cut(s) 976
SlaI CTCGAG 1 cut(s) 1490
SmaI CCCGGG 1 cut(s) 76
SmiMI CAYNNNNRTG 2 cut(s) 1063, 1467
SmlI CTYRAG 1 cut(s) 1490
SmoI CTYRAG 1 cut(s) 1490
SpeI ACTAGT 1 cut(s) 139
SphI GCATGC 1 cut(s) 743
Sse9I AATT 9 cut(s) 5, 165, 518, 692, 753, 757, 883, 1108, 1162
SsiI CCGC 7 cut(s) 53, 170, 240, 451, 1119, 1252, 1344
SspI AATATT 1 cut(s) 1264
SspMI CTAG 5 cut(s) 140, 287, 614, 845, 1406
StyD4I CCNGG 6 cut(s) 59, 73, 74, 743, 1135, 1327
StyI CCWWGG 2 cut(s) 481, 1484
TaaI ACNGT 4 cut(s) 667, 1130, 1151, 1541
TaiI ACGT 4 cut(s) 365, 874, 1277, 1319
TaqI TCGA 8 cut(s) 213, 330, 553, 586, 976, 1194, 1414, 1491
TaqII GACCGA 1 cut(s) 1444
TasI AATT 9 cut(s) 5, 165, 518, 692, 753, 757, 883, 1108, 1162
TatI WGTACW 1 cut(s) 1042
TauI GCSGC 1 cut(s) 1255
TfiI GAWTC 7 cut(s) 185, 251, 455, 905, 927, 978, 1569
Tru1I TTAA 6 cut(s) 308, 339, 756, 786, 1289, 1296
Tru9I TTAA 6 cut(s) 308, 339, 756, 786, 1289, 1296
TscAI CASTG 5 cut(s) 199, 288, 780, 1156, 1474
TseFI GTSAC 4 cut(s) 245, 709, 1121, 1151
TseI GCWGC 3 cut(s) 641, 951, 1448
Tsp45I GTSAC 4 cut(s) 245, 709, 1121, 1151
TspDTI ATGAA 5 cut(s) 17, 980, 1101, 1173, 1241
TspMI CCCGGG 1 cut(s) 74
TspRI CASTG 5 cut(s) 199, 288, 780, 1156, 1474
VspI ATTAAT 1 cut(s) 756
XagI CCTNNNNNAGG 1 cut(s) 106
XapI RAATTY 2 cut(s) 5, 883
XceI RCATGY 2 cut(s) 743, 1535
XcmI CCANNNNNNNNNTGG 2 cut(s) 741, 1482
XhoI CTCGAG 1 cut(s) 1490
XmaI CCCGGG 1 cut(s) 74
XspI CTAG 5 cut(s) 140, 287, 614, 845, 1406
Zsp2I ATGCAT 1 cut(s) 741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.