MD17G1189000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0015989
Physical Location & Seq
Forward (+)
22547048 .. 22550763
3716 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1189000.v1.1.491

Sequence Viewer

Length: 1347 bp
ATGCTACTCTCTCCGTCGAGCTCCACCCAAAGATCCCCCACCAAAATGCTCGATCGCGTCCTGTCCTCGCGCCGATACGCGCCGTACGCAGACGAATCCGCCGTGGAAAACGACGTCGTCGCCGACGAGTCCAAGATCAAGAAGCACCTCCCCTTCTCCTTCTTCACCACCCGAATCACCAACTACTTGACCCGAACCGGACCCGTCTGGCCCTGCCTCCTCATCCTCGCCCTTGTCCTCCTTCTCATCTTCTCCCTCATCTTCAACTCCCGGAGATTCGTCTGCGTCTCGCCTTACGACCCGGTTTCTCGCACCGGCTTCTTCGGGTTCGATGGCCTCGAATCGGATTTCGGATCACTCGGCGTGCCTTGGTGCAGATCGAAACACGGAAAAACAGTTGAGTGGACAACAAAGGATTTAATCAAGGGCTTGGAAGTATTTATACCTGTATATGAAACCCGACCGATAAAAAACAACATGTACGGGATGGGTTTTGACCACAGCTTTGGGCTCTGGTTCATTGCCCAGTGGCTGAAGCCGGATCTAATGATTGAGAGTGGCGCTTTCAAGGGTCATTCCACTTGGGTTTTGCGACAAGCGATGCCAGACACTCCAATTATTACCCTCACACCCAAGCATCCTGGGAAGTTTCTAAAGAAGGGCCCTGCATATGTTGATGGAAACTGCACTTACTTCACCGGAAAGGATTTTTTGGATTTTGGAAATGTTGATTGGGGAAAAGTGATGAAGAAACATGGGATTACTGATCTCAGCCGTGTTCTTATTTTTTTTGATGATCATCAGAATGAACTGAAAAGAGTAACGCAGGCTCTGAAAGCTGGTTTTCAGCACATAATTTTCGAGGATAATTATGATACTGGAACTGGAGATCATTATTCCTTACGGCAGATATGTGATCAATCCTATATCAGAGGTGGTCACAGCTGCTTTAAAGACAGCGATGAAGCTAGGATTAGGTCAAAAAGGAAGAAGTTCTGGGAGAAAGCGGTGAATATTGAAGAACTTTGTGGGCCTGGTGAAGCATGGTGGGGTGTTAGAGGATACATGCATGATAACTTTACTAAAAGTAGTAAGAAGATTTCCTATTCAGAACATTTTCAGAATAGTCGGTTTGTGGAATCGATTCTTGATGTTTACTGGGAGGTGCCACCAGTGGCAGGTCCATCCCTCACTCATCAAACTAGATATGATCCTGCTCGTGCCGTAACTCCTATTGTTGAAGATGGCCGGTATGGCTTATTCAATCGGCTTGGCTTAACCAGACTCGACCCTTCTGTATTTAATGGATATACCCAGATGGTTTACGTTCAGATATCAAACCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

449

Amino Acids

51.28

Weight (kDa)

8.85

Isoelectric Point (pI)

38.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015989)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G16200
fragaria_vesca FvH4_6g31940
malus_domestica MD09G1207900.v1.1 MD17G1189000.v1.1
prunus_persica Prupe.3G064100_v2.0.a1
pyrus_communis pycom09g12410 pycom17g19730
rosa_chinensis RchiOBHm_Chr2g0139361
rosa_laevigata RLG00000019808
rosa_multiflora Rmu_sc0004390.1_g000004
rosa_roxburghii Rroxscaffold_2G00105440
rosa_rugosa Rorug02G0357700
rosa_samantha Rh2DG426500
rosa_wichuraiana Rw2G032980 Rw2G033240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 117
Acc36I ACCTGC 1 cut(s) 1169
AccB1I GGYRCC 1 cut(s) 1165
AccII CGCG 3 cut(s) 57, 70, 80
AciI CCGC 2 cut(s) 99, 1007
AclWI GGATC 4 cut(s) 27, 361, 549, 1205
AcoI YGGCCR 1 cut(s) 1246
AcuI CTGAAG 1 cut(s) 554
AcyI GRCGYC 1 cut(s) 114
AfaI GTAC 2 cut(s) 86, 482
AfiI CCNNNNNNNGG 2 cut(s) 343, 1178
AflIII ACRYGT 1 cut(s) 477
AgsI TTSAA 5 cut(s) 265, 568, 1019, 1241, 1264
AjnI CCWGG 2 cut(s) 640, 1033
AjuI GAANNNNNNNTTGG 2 cut(s) 715, 747
AluBI AGCT 5 cut(s) 21, 504, 839, 945, 968
AluI AGCT 5 cut(s) 21, 504, 839, 945, 968
Alw21I GWGCWC 1 cut(s) 23
Alw26I GTCTC 1 cut(s) 292
AlwI GGATC 4 cut(s) 27, 361, 549, 1205
AlwNI CAGNNNCTG 2 cut(s) 532, 832
AoxI GGCC 5 cut(s) 209, 334, 661, 1031, 1246
ApaI GGGCCC 1 cut(s) 665
ApeKI GCWGC 1 cut(s) 945
ArsI GACNNNNNNTTYG 2 cut(s) 488, 520
Asp700I GAANNNNTTC 3 cut(s) 992, 1116, 1143
AspLEI GCGC 3 cut(s) 72, 82, 563
AspS9I GGNCC 6 cut(s) 200, 210, 661, 662, 1031, 1181
AsuC2I CCSGG 2 cut(s) 271, 302
AsuHPI GGTGA 5 cut(s) 157, 169, 688, 1021, 1049
AvaII GGWCC 2 cut(s) 200, 1181
BaeGI GKGCMC 1 cut(s) 665
BanI GGYRCC 1 cut(s) 1165
BanII GRGCYC 3 cut(s) 23, 513, 665
BarI GAAGNNNNNNTAC 2 cut(s) 426, 458
BauI CACGAG 1 cut(s) 1218
Bbv12I GWGCWC 1 cut(s) 23
BbvI GCAGC 1 cut(s) 932
BccI CCATC 6 cut(s) 326, 481, 671, 1192, 1238, 1312
BceAI ACGGC 5 cut(s) 67, 86, 759, 920, 1208
BciT130I CCWGG 2 cut(s) 642, 1035
BciVI GTATCC 1 cut(s) 1055
BclI TGATCA 2 cut(s) 796, 916
BcnI CCSGG 2 cut(s) 271, 302
BcoDI GTCTC 1 cut(s) 292
BfaI CTAG 2 cut(s) 969, 1203
BfoI RGCGCY 1 cut(s) 564
BfuAI ACCTGC 1 cut(s) 1169
BfuI GTATCC 1 cut(s) 1055
BglI GCCNNNNNGGC 1 cut(s) 1254
BisI GCNGC 1 cut(s) 946
BlsI GCNGC 1 cut(s) 947
Bme1390I CCNGG 4 cut(s) 271, 302, 642, 1035
Bme18I GGWCC 2 cut(s) 200, 1181
BmgT120I GGNCC 6 cut(s) 200, 210, 661, 662, 1031, 1181
BmiI GGNNCC 3 cut(s) 202, 663, 1167
BmrFI CCNGG 4 cut(s) 271, 302, 642, 1035
BmrI ACTGGG 2 cut(s) 520, 1168
BmsI GCATC 2 cut(s) 591, 646
BmuI ACTGGG 2 cut(s) 520, 1168
BpmI CTGGAG 1 cut(s) 906
BpuMI CCSGG 2 cut(s) 271, 302
Bsa29I ATCGAT 1 cut(s) 1142
BsaBI GATNNNNATC 1 cut(s) 798
BsaHI GRCGYC 1 cut(s) 114
BsaJI CCNNGG 3 cut(s) 102, 368, 641
BsaWI WCCGGW 2 cut(s) 197, 698
Bsc4I CCNNNNNNNGG 2 cut(s) 343, 1178
Bse118I RCCGGY 2 cut(s) 314, 1248
Bse1I ACTGG 6 cut(s) 526, 883, 889, 1163, 1172, 1342
Bse3DI GCAATG 1 cut(s) 519
Bse8I GATNNNNATC 1 cut(s) 798
BseBI CCWGG 2 cut(s) 642, 1035
BseCI ATCGAT 1 cut(s) 1142
BseDI CCNNGG 3 cut(s) 102, 368, 641
BseGI GGATG 4 cut(s) 222, 492, 637, 1184
BseJI GATNNNNATC 1 cut(s) 798
BseLI CCNNNNNNNGG 2 cut(s) 343, 1178
BseMI GCAATG 1 cut(s) 519
BseMII CTCAG 1 cut(s) 784
BseNI ACTGG 6 cut(s) 526, 883, 889, 1163, 1172, 1342
BseRI GAGGAG 1 cut(s) 209
BseSI GKGCMC 1 cut(s) 665
BseXI GCAGC 1 cut(s) 932
BsgI GTGCAG 2 cut(s) 394, 670
Bsh1236I CGCG 3 cut(s) 57, 70, 80
Bsh1285I CGRYCG 2 cut(s) 55, 464
BshFI GGCC 5 cut(s) 211, 336, 663, 1033, 1248
BshNI GGYRCC 1 cut(s) 1165
BshVI ATCGAT 1 cut(s) 1142
BsiEI CGRYCG 2 cut(s) 55, 464
BsiHKAI GWGCWC 1 cut(s) 23
BsiSI CCGG 7 cut(s) 198, 271, 302, 315, 539, 699, 1249
BsiWI CGTACG 1 cut(s) 84
BslI CCNNNNNNNGG 2 cut(s) 343, 1178
BsmAI GTCTC 1 cut(s) 292
BsmBI CGTCTC 1 cut(s) 292
BsnI GGCC 5 cut(s) 211, 336, 663, 1033, 1248
Bsp120I GGGCCC 1 cut(s) 661
Bsp1286I GDGCHC 3 cut(s) 23, 513, 665
BspACI CCGC 2 cut(s) 99, 1007
BspANI GGCC 5 cut(s) 211, 336, 663, 1033, 1248
BspCNI CTCAG 1 cut(s) 783
BspDI ATCGAT 1 cut(s) 1142
BspFNI CGCG 3 cut(s) 57, 70, 80
BspLI GGNNCC 3 cut(s) 202, 663, 1167
BspMI ACCTGC 1 cut(s) 1169
BspPI GGATC 4 cut(s) 27, 361, 549, 1205
BspT107I GGYRCC 1 cut(s) 1165
BsrDI GCAATG 1 cut(s) 519
BsrFI RCCGGY 2 cut(s) 314, 1248
BsrI ACTGG 6 cut(s) 526, 883, 889, 1163, 1172, 1342
BssAI RCCGGY 2 cut(s) 314, 1248
BssECI CCNNGG 3 cut(s) 102, 368, 641
BssNI GRCGYC 1 cut(s) 114
BssSI CACGAG 1 cut(s) 1218
BssT1I CCWWGG 1 cut(s) 368
Bst2BI CACGAG 1 cut(s) 1218
Bst2UI CCWGG 2 cut(s) 642, 1035
Bst4CI ACNGT 1 cut(s) 397
BstACI GRCGYC 1 cut(s) 114
BstC8I GCNNGC 2 cut(s) 365, 828
BstDEI CTNAG 1 cut(s) 770
BstDSI CCRYGG 1 cut(s) 102
BstF5I GGATG 4 cut(s) 222, 492, 637, 1184
BstFNI CGCG 3 cut(s) 57, 70, 80
BstH2I RGCGCY 1 cut(s) 564
BstHHI GCGC 3 cut(s) 72, 82, 563
BstMAI GTCTC 1 cut(s) 292
BstMCI CGRYCG 2 cut(s) 55, 464
BstMWI GCNNNNNNNGC 3 cut(s) 86, 836, 1254
BstNI CCWGG 2 cut(s) 642, 1035
BstNSI RCATGY 2 cut(s) 481, 1069
BstSCI CCNGG 4 cut(s) 269, 300, 640, 1033
BstSLI GKGCMC 1 cut(s) 665
BstUI CGCG 3 cut(s) 57, 70, 80
BstV1I GCAGC 1 cut(s) 932
BstX2I RGATCY 2 cut(s) 32, 541
BstXI CCANNNNNNTGG 1 cut(s) 506
BstYI RGATCY 2 cut(s) 32, 541
Bsu15I ATCGAT 1 cut(s) 1142
BsuI GTATCC 1 cut(s) 1055
BsuRI GGCC 5 cut(s) 211, 336, 663, 1033, 1248
BsuTUI ATCGAT 1 cut(s) 1142
BtgI CCRYGG 1 cut(s) 102
BtgZI GCGATG 2 cut(s) 614, 975
BtsCI GGATG 4 cut(s) 222, 492, 637, 1184
BtsIMutI CAGTG 2 cut(s) 533, 1179
BveI ACCTGC 1 cut(s) 1169
Cac8I GCNNGC 2 cut(s) 365, 828
CaiI CAGNNNCTG 2 cut(s) 532, 832
CfoI GCGC 3 cut(s) 72, 82, 563
Cfr10I RCCGGY 2 cut(s) 314, 1248
Cfr13I GGNCC 6 cut(s) 200, 210, 661, 662, 1031, 1181
ClaI ATCGAT 1 cut(s) 1142
CseI GACGC 2 cut(s) 46, 274
Csp6I GTAC 2 cut(s) 85, 481
CviAII CATG 5 cut(s) 478, 755, 1044, 1066, 1070
CviQI GTAC 2 cut(s) 85, 481
DdeI CTNAG 1 cut(s) 770
DraI TTTAAA 1 cut(s) 952
EaeI YGGCCR 1 cut(s) 1246
EciI GGCGGA 1 cut(s) 88
Ecl136II GAGCTC 1 cut(s) 21
Eco130I CCWWGG 1 cut(s) 368
Eco24I GRGCYC 3 cut(s) 23, 513, 665
Eco32I GATATC 1 cut(s) 1335
Eco47I GGWCC 2 cut(s) 200, 1181
Eco53kI GAGCTC 1 cut(s) 21
Eco57I CTGAAG 1 cut(s) 554
EcoICRI GAGCTC 1 cut(s) 21
EcoO109I RGGNCCY 2 cut(s) 661, 662
EcoRII CCWGG 2 cut(s) 640, 1033
EcoRV GATATC 1 cut(s) 1335
EcoT14I CCWWGG 1 cut(s) 368
EcoT22I ATGCAT 1 cut(s) 1071
EcoT38I GRGCYC 3 cut(s) 23, 513, 665
ErhI CCWWGG 1 cut(s) 368
Esp3I CGTCTC 1 cut(s) 292
FaeI CATG 5 cut(s) 481, 758, 1047, 1069, 1073
FatI CATG 5 cut(s) 477, 754, 1043, 1065, 1069
FauNDI CATATG 1 cut(s) 670
FbaI TGATCA 2 cut(s) 796, 916
Fnu4HI GCNGC 1 cut(s) 946
FokI GGATG 4 cut(s) 209, 499, 624, 1171
FriOI GRGCYC 3 cut(s) 23, 513, 665
Fsp4HI GCNGC 1 cut(s) 946
FspBI CTAG 2 cut(s) 969, 1203
GlaI GCGC 3 cut(s) 71, 81, 562
GluI GCNGC 1 cut(s) 946
GsuI CTGGAG 1 cut(s) 906
HaeII RGCGCY 1 cut(s) 564
HaeIII GGCC 5 cut(s) 211, 336, 663, 1033, 1248
HapII CCGG 7 cut(s) 198, 271, 302, 315, 539, 699, 1249
HgaI GACGC 2 cut(s) 46, 274
HhaI GCGC 3 cut(s) 72, 82, 563
Hin1I GRCGYC 1 cut(s) 114
Hin1II CATG 5 cut(s) 481, 758, 1047, 1069, 1073
Hin6I GCGC 3 cut(s) 70, 80, 561
HinP1I GCGC 3 cut(s) 70, 80, 561
HinfI GANTC 8 cut(s) 95, 128, 174, 276, 341, 1139, 1144, 1284
HpaII CCGG 7 cut(s) 198, 271, 302, 315, 539, 699, 1249
HphI GGTGA 5 cut(s) 157, 169, 688, 1021, 1049
Hpy166II GTNNAC 3 cut(s) 405, 1156, 1324
Hpy188I TCNGA 8 cut(s) 346, 353, 804, 834, 932, 1111, 1122, 1332
Hpy188III TCNNGA 2 cut(s) 139, 1148
Hpy8I GTNNAC 3 cut(s) 405, 1156, 1324
Hpy99I CGWCG 5 cut(s) 19, 116, 119, 122, 128
HpyAV CCTTC 5 cut(s) 163, 169, 251, 652, 1302
HpyCH4III ACNGT 1 cut(s) 397
HpyCH4IV ACGT 2 cut(s) 114, 1326
HpyCH4V TGCA 4 cut(s) 375, 668, 687, 1069
HpyF10VI GCNNNNNNNGC 3 cut(s) 86, 836, 1254
HpyF3I CTNAG 1 cut(s) 770
HpySE526I ACGT 2 cut(s) 114, 1326
Hsp92I GRCGYC 1 cut(s) 114
Hsp92II CATG 5 cut(s) 481, 758, 1047, 1069, 1073
HspAI GCGC 3 cut(s) 70, 80, 561
Ksp22I TGATCA 2 cut(s) 796, 916
LmnI GCTCC 1 cut(s) 26
Lsp1109I GCAGC 1 cut(s) 932
LweI GCATC 2 cut(s) 591, 646
MaeI CTAG 2 cut(s) 969, 1203
MaeII ACGT 2 cut(s) 114, 1326
MaeIII GTNAC 3 cut(s) 820, 938, 1225
MboII GAAGA 9 cut(s) 154, 241, 253, 313, 760, 1000, 1031, 1108, 1253
MflI RGATCY 2 cut(s) 32, 541
MhlI GDGCHC 3 cut(s) 23, 513, 665
MluCI AATT 3 cut(s) 615, 855, 868
MlyI GAGTC 2 cut(s) 137, 1278
Mph1103I ATGCAT 1 cut(s) 1071
MroXI GAANNNNTTC 3 cut(s) 992, 1116, 1143
MseI TTAA 4 cut(s) 419, 951, 1277, 1302
MslI CAYNNNNRTG 2 cut(s) 44, 804
MspA1I CMGCKG 1 cut(s) 945
MspI CCGG 7 cut(s) 198, 271, 302, 315, 539, 699, 1249
MspR9I CCNGG 4 cut(s) 271, 302, 642, 1035
MvaI CCWGG 2 cut(s) 642, 1035
MvnI CGCG 3 cut(s) 57, 70, 80
MwoI GCNNNNNNNGC 3 cut(s) 86, 836, 1254
NciI CCSGG 2 cut(s) 271, 302
NdeI CATATG 1 cut(s) 670
NlaIII CATG 5 cut(s) 481, 758, 1047, 1069, 1073
NlaIV GGNNCC 3 cut(s) 202, 663, 1167
NmeAIII GCCGAG 1 cut(s) 339
NmuCI GTSAC 1 cut(s) 938
NsiI ATGCAT 1 cut(s) 1071
NspI RCATGY 2 cut(s) 481, 1069
PciI ACATGT 1 cut(s) 477
PcsI WCGNNNNNNNCGW 4 cut(s) 99, 120, 123, 336
PdmI GAANNNNTTC 3 cut(s) 992, 1116, 1143
PfeI GAWTC 6 cut(s) 95, 174, 276, 341, 1139, 1144
Pfl23II CGTACG 1 cut(s) 84
PflFI GACNNNGTC 1 cut(s) 116
PfoI TCCNGGA 1 cut(s) 269
PkrI GCNGC 1 cut(s) 947
Ple19I CGATCG 1 cut(s) 55
PleI GAGTC 2 cut(s) 136, 1278
PpsI GAGTC 2 cut(s) 136, 1278
PscI ACATGT 1 cut(s) 477
Psp124BI GAGCTC 1 cut(s) 23
Psp6I CCWGG 2 cut(s) 640, 1033
PspGI CCWGG 2 cut(s) 640, 1033
PspLI CGTACG 1 cut(s) 84
PspN4I GGNNCC 3 cut(s) 202, 663, 1167
PspOMI GGGCCC 1 cut(s) 661
PspPI GGNCC 6 cut(s) 200, 210, 661, 662, 1031, 1181
PstNI CAGNNNCTG 2 cut(s) 532, 832
PsuI RGATCY 2 cut(s) 32, 541
PsyI GACNNNGTC 1 cut(s) 116
PvuI CGATCG 1 cut(s) 55
PvuII CAGCTG 1 cut(s) 945
RsaI GTAC 2 cut(s) 86, 482
RsaNI GTAC 2 cut(s) 85, 481
RseI CAYNNNNRTG 2 cut(s) 44, 804
SacI GAGCTC 1 cut(s) 23
SaqAI TTAA 4 cut(s) 419, 951, 1277, 1302
SatI GCNGC 1 cut(s) 946
Sau96I GGNCC 6 cut(s) 200, 210, 661, 662, 1031, 1181
SchI GAGTC 2 cut(s) 137, 1278
ScrFI CCNGG 4 cut(s) 271, 302, 642, 1035
SduI GDGCHC 3 cut(s) 23, 513, 665
SfaNI GCATC 2 cut(s) 591, 646
SinI GGWCC 2 cut(s) 200, 1181
SmiMI CAYNNNNRTG 2 cut(s) 44, 804
Sse9I AATT 3 cut(s) 615, 855, 868
SsiI CCGC 2 cut(s) 99, 1007
SspI AATATT 1 cut(s) 1015
SspMI CTAG 2 cut(s) 969, 1203
SstI GAGCTC 1 cut(s) 23
StyD4I CCNGG 4 cut(s) 269, 300, 640, 1033
StyI CCWWGG 1 cut(s) 368
TaaI ACNGT 1 cut(s) 397
TaiI ACGT 2 cut(s) 117, 1329
TaqI TCGA 8 cut(s) 17, 51, 330, 339, 380, 861, 1142, 1287
TaqII GACCGA 1 cut(s) 478
TasI AATT 3 cut(s) 615, 855, 868
TfiI GAWTC 6 cut(s) 95, 174, 276, 341, 1139, 1144
Tru1I TTAA 4 cut(s) 419, 951, 1277, 1302
Tru9I TTAA 4 cut(s) 419, 951, 1277, 1302
TscAI CASTG 2 cut(s) 533, 1179
TseFI GTSAC 1 cut(s) 938
TseI GCWGC 1 cut(s) 945
Tsp45I GTSAC 1 cut(s) 938
TspDTI ATGAA 5 cut(s) 468, 508, 761, 822, 978
TspGWI ACGGA 1 cut(s) 402
TspRI CASTG 2 cut(s) 533, 1179
Tth111I GACNNNGTC 1 cut(s) 116
VpaK11BI GGWCC 2 cut(s) 200, 1181
XceI RCATGY 2 cut(s) 481, 1069
XmnI GAANNNNTTC 3 cut(s) 992, 1116, 1143
XspI CTAG 2 cut(s) 969, 1203
ZraI GACGTC 1 cut(s) 115
Zsp2I ATGCAT 1 cut(s) 1071
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.