MD17G1223300.v1.1

Photosynthetic NDH subunit of subcomplex B 1, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0013329
Physical Location & Seq
Forward (+)
27227720 .. 27230805
3086 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1223300.v1.1.491

Sequence Viewer

Length: 1392 bp
ATGGCCACTACAACTTCTCTGCTTCCCAAATCCTTCCCACCATTTCTCACCAACGCACCTTCATTCCCCTCAACCCTTTTCACCAAGCTCTCGTCTTTCAACCCACAGGACCACCCCCATTGCACAAAAAGGCCCAACCTTCAAACAAATGCCAAGAAGAAGAACCCCTGGCTCGACCCTTTTGATGATGGGGAGGACCCTGACATGGAGTACGGATCCCTCTTCGCGGACGGGAAGCAGGACGAGGACGATAGGCCACCCGAAAACCCCGATAATCCATACGGGTTCTTGAAGTTCCCAATGGGGTACAGTGTGGAAGTAGCATCACTGGCATTGAAAGTGAGGGGGGATGTTAGGAGGTGTTGCTGTGTGGTTTCTGGTGGTGTGTATGAGAATTTGCTGTATTTTCCCGTAATTCAGTTGCTTAAGGACCGGTATCCCGGCGTGCAAGTGGATGTGGTGGCTTCGGCCAGAGGGAAGCAAGCTTATGAGTTGAATAAGAATGTGAGATGGGCTGATGTGTATGATCCTGATGATCATTTTCCTGAGCCTGCTGAGTACACTGACATGGTTGGACTCCTTAAGAGTAGGTACTACGACATGATCTTATCAACCAAGCTAGCAGGTCTCGGCCATGGAATATTTTTGTTCATGACAACAGCCAGAGAGAGGGTGAGCTACATTTATCCAAATGTAAACGCTGCAGGAGCTGGATTGTTTTTATCTGAAACATTTAGACCGAATGGTATGAATCTCGCTGAAGGCGGGTATAACATGTATCACCAGATGGTGGATTGGTTGGGGAGACCAGTGCGGGATGTGCCACGACAAGCTTTGCCCCCGCTTAAAATATCAATTTCAAAGAAGCTAAAGCAGACTGTAGAGTCAAAATACAAGAAAGCAGGTGTAGATAAAGGAAAGTATATTGTGATTCACGGGTTGGAGTCGGATTCAAAAGCCTCAATGCAGTCTAAGGGGGATAGTGATAGCTTGCTACCCATCGAAACTTGGGCTGCAATAGTCCGCGGTATAAGGGAATTTAGGCCAGTTTTTGTCATTCCACATGAGAAAGAAAGGGAAAATGTGGAGGAAATAGTTGGAGAAGATACCAGCATTGTGTTCATAACAACCCCTGGGCAGCTTGCCGCTCTTATCAATGACTCGGCTGGTGTGATAACTACAAATACCGCAGCTATCCAACTTGCAAATGCACGCCAAAAACCCTGCATTGCATTATTTGGCTCTGCAGAGAAGGGGAAACTATTCGTTCCGAACGCAGAGGAGAAGAACTGTGTTATTGTTTCATCCAAGACAAAAAAGTTAAAGGACATTGACACTGAAGCTGTTAAAAATGCACTTCCAATTTTCAATGTGTCCCTAGCTCTAGTCTAA

Protein Analysis

464

Amino Acids

51.36

Weight (kDa)

6.68

Isoelectric Point (pI)

29.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_9 PF01075 332 - 443 9.1e-07 Glycosyltransferase family 9 (heptosyltransferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013329)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15980
fragaria_vesca FvH4_6g28640 FvH4_6g28640 FvH4_6g28640
malus_domestica MD17G1223300.v1.1
prunus_persica Prupe.3G098200_v2.0.a1
pyrus_communis pycom17g22730
rosa_chinensis RchiOBHm_Chr2g0132981
rosa_laevigata RLG00000019301
rosa_multiflora Rmu_sc0007314.1_g000011 Rmu_sc0019447.1_g000001
rosa_roxburghii Rroxscaffold_2G00111580
rosa_rugosa Rorug02G0309100
rosa_samantha Rh2AG361300 Rh2BG367100 Rh2CG344600 Rh2DG384100
rosa_wichuraiana Rw2G029380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 893
AasI GACNNNNNNGTC 1 cut(s) 883
Acc36I ACCTGC 2 cut(s) 614, 893
AccB7I CCANNNNNTGG 1 cut(s) 790
AccBSI CCGCTC 1 cut(s) 1148
AccII CGCG 2 cut(s) 227, 1026
AciI CCGC 8 cut(s) 227, 765, 814, 842, 1024, 1026, 1146, 1188
AclWI GGATC 3 cut(s) 210, 223, 521
AcoI YGGCCR 3 cut(s) 3, 468, 631
AcsI RAATTY 2 cut(s) 394, 1037
AcuI CTGAAG 2 cut(s) 780, 1359
AfaI GTAC 4 cut(s) 212, 308, 560, 593
AfiI CCNNNNNNNGG 4 cut(s) 205, 226, 669, 790
AflII CTTAAG 2 cut(s) 425, 581
AflIII ACRYGT 1 cut(s) 774
AgeI ACCGGT 1 cut(s) 432
AgsI TTSAA 8 cut(s) 100, 143, 292, 337, 496, 861, 954, 1369
AjnI CCWGG 2 cut(s) 167, 1132
Alw26I GTCTC 2 cut(s) 632, 799
AlwI GGATC 3 cut(s) 210, 223, 521
AlwNI CAGNNNCTG 1 cut(s) 710
AoxI GGCC 6 cut(s) 3, 131, 254, 468, 631, 1043
ApeKI GCWGC 4 cut(s) 701, 1013, 1138, 1190
ApoI RAATTY 2 cut(s) 394, 1037
AsiGI ACCGGT 1 cut(s) 432
Asp700I GAANNNNTTC 1 cut(s) 1262
AspS9I GGNCC 4 cut(s) 109, 132, 196, 430
AsuC2I CCSGG 1 cut(s) 441
AsuHPI GGTGA 4 cut(s) 40, 73, 685, 773
AsuNHI GCTAGC 1 cut(s) 619
AvaII GGWCC 3 cut(s) 109, 196, 430
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 215
BbvI GCAGC 4 cut(s) 688, 1000, 1150, 1202
BccI CCATC 4 cut(s) 182, 504, 781, 1007
BciT130I CCWGG 2 cut(s) 169, 1134
BciVI GTATCC 1 cut(s) 447
BclI TGATCA 1 cut(s) 535
BcnI CCSGG 1 cut(s) 441
BcoDI GTCTC 2 cut(s) 632, 799
BfaI CTAG 3 cut(s) 620, 1379, 1385
BfmI CTRYAG 3 cut(s) 702, 879, 1245
BfrI CTTAAG 2 cut(s) 425, 581
BfuAI ACCTGC 2 cut(s) 614, 893
BfuI GTATCC 1 cut(s) 447
BisI GCNGC 5 cut(s) 702, 1014, 1139, 1146, 1191
BlsI GCNGC 5 cut(s) 703, 1015, 1140, 1147, 1192
Bme1390I CCNGG 3 cut(s) 169, 441, 1134
Bme18I GGWCC 3 cut(s) 109, 196, 430
BmgT120I GGNCC 4 cut(s) 109, 132, 196, 430
BmiI GGNNCC 2 cut(s) 198, 217
BmrFI CCNGG 3 cut(s) 169, 441, 1134
BmsI GCATC 1 cut(s) 332
BmtI GCTAGC 1 cut(s) 623
Bpu10I CCTNAGC 1 cut(s) 546
BpuMI CCSGG 1 cut(s) 441
BsaI GGTCTC 2 cut(s) 632, 799
BsaJI CCNNGG 5 cut(s) 167, 634, 1024, 1132, 1133
BsaWI WCCGGW 1 cut(s) 432
Bsc4I CCNNNNNNNGG 4 cut(s) 205, 226, 669, 790
Bse118I RCCGGY 1 cut(s) 432
Bse1I ACTGG 3 cut(s) 333, 809, 1046
Bse3DI GCAATG 2 cut(s) 118, 1227
BseBI CCWGG 2 cut(s) 169, 1134
BseDI CCNNGG 5 cut(s) 167, 634, 1024, 1132, 1133
BseGI GGATG 4 cut(s) 355, 460, 823, 1304
BseLI CCNNNNNNNGG 4 cut(s) 205, 226, 669, 790
BseMI GCAATG 2 cut(s) 118, 1227
BseMII CTCAG 2 cut(s) 537, 546
BseNI ACTGG 3 cut(s) 333, 809, 1046
BseRI GAGGAG 1 cut(s) 1295
BseXI GCAGC 4 cut(s) 688, 1000, 1150, 1202
Bsh1236I CGCG 2 cut(s) 227, 1026
BshFI GGCC 6 cut(s) 5, 133, 256, 470, 633, 1045
BshTI ACCGGT 1 cut(s) 432
BsiSI CCGG 2 cut(s) 433, 441
BslFI GGGAC 1 cut(s) 1360
BslI CCNNNNNNNGG 4 cut(s) 205, 226, 669, 790
BsmAI GTCTC 2 cut(s) 632, 799
BsmFI GGGAC 1 cut(s) 1360
BsnI GGCC 6 cut(s) 5, 133, 256, 470, 633, 1045
Bso31I GGTCTC 2 cut(s) 632, 799
Bsp143I GATC 4 cut(s) 215, 526, 535, 603
Bsp19I CCATGG 1 cut(s) 634
BspACI CCGC 8 cut(s) 227, 765, 814, 842, 1024, 1026, 1146, 1188
BspANI GGCC 6 cut(s) 5, 133, 256, 470, 633, 1045
BspCNI CTCAG 2 cut(s) 538, 547
BspFNI CGCG 2 cut(s) 227, 1026
BspHI TCATGA 1 cut(s) 651
BspLI GGNNCC 2 cut(s) 198, 217
BspMAI CTGCAG 2 cut(s) 706, 1249
BspMI ACCTGC 2 cut(s) 614, 893
BspOI GCTAGC 1 cut(s) 623
BspPI GGATC 3 cut(s) 210, 223, 521
BspTI CTTAAG 2 cut(s) 425, 581
BspTNI GGTCTC 2 cut(s) 632, 799
BsrBI CCGCTC 1 cut(s) 1148
BsrDI GCAATG 2 cut(s) 118, 1227
BsrFI RCCGGY 1 cut(s) 432
BsrI ACTGG 3 cut(s) 333, 809, 1046
BssAI RCCGGY 1 cut(s) 432
BssECI CCNNGG 5 cut(s) 167, 634, 1024, 1132, 1133
BssMI GATC 4 cut(s) 215, 526, 535, 603
BssT1I CCWWGG 1 cut(s) 634
Bst2UI CCWGG 2 cut(s) 169, 1134
Bst4CI ACNGT 3 cut(s) 311, 880, 1292
Bst6I CTCTTC 1 cut(s) 227
BstAFI CTTAAG 2 cut(s) 425, 581
BstC8I GCNNGC 7 cut(s) 446, 483, 552, 621, 992, 1143, 1213
BstDEI CTNAG 3 cut(s) 546, 555, 972
BstDSI CCRYGG 2 cut(s) 634, 1024
BstF5I GGATG 4 cut(s) 355, 460, 823, 1304
BstFNI CGCG 2 cut(s) 227, 1026
BstKTI GATC 4 cut(s) 218, 529, 538, 606
BstMAI GTCTC 2 cut(s) 632, 799
BstMBI GATC 4 cut(s) 215, 526, 535, 603
BstMWI GCNNNNNNNGC 3 cut(s) 329, 707, 820
BstNI CCWGG 2 cut(s) 169, 1134
BstNSI RCATGY 1 cut(s) 778
BstSCI CCNGG 3 cut(s) 167, 439, 1132
BstSFI CTRYAG 3 cut(s) 702, 879, 1245
BstUI CGCG 2 cut(s) 227, 1026
BstV1I GCAGC 4 cut(s) 688, 1000, 1150, 1202
BstX2I RGATCY 1 cut(s) 215
BstYI RGATCY 1 cut(s) 215
BsuI GTATCC 1 cut(s) 447
BsuRI GGCC 6 cut(s) 5, 133, 256, 470, 633, 1045
BtgI CCRYGG 2 cut(s) 634, 1024
BtsCI GGATG 4 cut(s) 355, 460, 823, 1304
BtsIMutI CAGTG 5 cut(s) 316, 326, 561, 816, 1335
BveI ACCTGC 2 cut(s) 614, 893
Cac8I GCNNGC 7 cut(s) 446, 483, 552, 621, 992, 1143, 1213
CaiI CAGNNNCTG 1 cut(s) 710
CciI TCATGA 1 cut(s) 651
Cfr10I RCCGGY 1 cut(s) 432
Cfr13I GGNCC 4 cut(s) 109, 132, 196, 430
Cfr42I CCGCGG 1 cut(s) 1027
Csp6I GTAC 4 cut(s) 211, 307, 559, 592
CspAI ACCGGT 1 cut(s) 432
CspCI CAANNNNNGTGG 2 cut(s) 101, 136
CviAII CATG 7 cut(s) 205, 568, 601, 635, 652, 775, 1064
CviQI GTAC 4 cut(s) 211, 307, 559, 592
DdeI CTNAG 3 cut(s) 546, 555, 972
DpnI GATC 4 cut(s) 217, 528, 537, 605
DpnII GATC 4 cut(s) 215, 526, 535, 603
DrdI GACNNNNNNGTC 1 cut(s) 883
DseDI GACNNNNNNGTC 1 cut(s) 883
EaeI YGGCCR 3 cut(s) 3, 468, 631
Eam1104I CTCTTC 1 cut(s) 227
EarI CTCTTC 1 cut(s) 227
Eco130I CCWWGG 1 cut(s) 634
Eco31I GGTCTC 2 cut(s) 632, 799
Eco47I GGWCC 3 cut(s) 109, 196, 430
Eco57I CTGAAG 2 cut(s) 780, 1359
EcoO109I RGGNCCY 1 cut(s) 196
EcoRII CCWGG 2 cut(s) 167, 1132
EcoT14I CCWWGG 1 cut(s) 634
ErhI CCWWGG 1 cut(s) 634
FaeI CATG 7 cut(s) 208, 571, 604, 638, 655, 778, 1067
FaqI GGGAC 1 cut(s) 1360
FatI CATG 7 cut(s) 204, 567, 600, 634, 651, 774, 1063
FauI CCCGC 3 cut(s) 758, 807, 849
FbaI TGATCA 1 cut(s) 535
Fnu4HI GCNGC 5 cut(s) 702, 1014, 1139, 1146, 1191
FokI GGATG 4 cut(s) 362, 467, 830, 1291
Fsp4HI GCNGC 5 cut(s) 702, 1014, 1139, 1146, 1191
FspBI CTAG 3 cut(s) 620, 1379, 1385
GluI GCNGC 5 cut(s) 702, 1014, 1139, 1146, 1191
HaeIII GGCC 6 cut(s) 5, 133, 256, 470, 633, 1045
HapII CCGG 2 cut(s) 433, 441
Hin1II CATG 7 cut(s) 208, 571, 604, 638, 655, 778, 1067
HindIII AAGCTT 2 cut(s) 483, 831
HinfI GANTC 7 cut(s) 576, 751, 884, 931, 944, 950, 1160
HpaII CCGG 2 cut(s) 433, 441
HphI GGTGA 4 cut(s) 40, 73, 685, 773
Hpy166II GTNNAC 2 cut(s) 561, 697
Hpy188I TCNGA 3 cut(s) 727, 949, 1272
Hpy188III TCNNGA 4 cut(s) 289, 530, 545, 652
Hpy8I GTNNAC 2 cut(s) 561, 697
HpyAV CCTTC 5 cut(s) 43, 69, 149, 755, 1246
HpyCH4III ACNGT 3 cut(s) 311, 880, 1292
HpyF10VI GCNNNNNNNGC 3 cut(s) 329, 707, 820
HpyF3I CTNAG 3 cut(s) 546, 555, 972
Hsp92II CATG 7 cut(s) 208, 571, 604, 638, 655, 778, 1067
Ksp22I TGATCA 1 cut(s) 535
KspI CCGCGG 1 cut(s) 1027
Kzo9I GATC 4 cut(s) 215, 526, 535, 603
LmnI GCTCC 1 cut(s) 707
Lsp1109I GCAGC 4 cut(s) 688, 1000, 1150, 1202
LweI GCATC 1 cut(s) 332
MaeI CTAG 3 cut(s) 620, 1379, 1385
MalI GATC 4 cut(s) 217, 528, 537, 605
MbiI CCGCTC 1 cut(s) 1148
MboI GATC 4 cut(s) 215, 526, 535, 603
MboII GAAGA 5 cut(s) 169, 172, 214, 1115, 1297
MflI RGATCY 1 cut(s) 215
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 5 cut(s) 394, 414, 855, 1037, 1362
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 4 cut(s) 570, 893, 953, 1154
MmeI TCCRAC 5 cut(s) 553, 921, 927, 1078, 1222
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 1262
MscI TGGCCA 1 cut(s) 5
MseI TTAA 5 cut(s) 426, 582, 846, 1322, 1347
MslI CAYNNNNRTG 1 cut(s) 566
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 1026
MspCI CTTAAG 2 cut(s) 425, 581
MspI CCGG 2 cut(s) 433, 441
MspR9I CCNGG 3 cut(s) 169, 441, 1134
MvaI CCWGG 2 cut(s) 169, 1134
MvnI CGCG 2 cut(s) 227, 1026
MwoI GCNNNNNNNGC 3 cut(s) 329, 707, 820
NciI CCSGG 1 cut(s) 441
NcoI CCATGG 1 cut(s) 634
NdeII GATC 4 cut(s) 215, 526, 535, 603
NheI GCTAGC 1 cut(s) 619
NlaIII CATG 7 cut(s) 208, 571, 604, 638, 655, 778, 1067
NlaIV GGNNCC 2 cut(s) 198, 217
NmeAIII GCCGAG 2 cut(s) 609, 1142
NspI RCATGY 1 cut(s) 778
PagI TCATGA 1 cut(s) 651
PaqCI CACCTGC 1 cut(s) 893
PasI CCCWGGG 1 cut(s) 1133
PciI ACATGT 1 cut(s) 774
PdmI GAANNNNTTC 1 cut(s) 1262
PfeI GAWTC 3 cut(s) 751, 931, 950
PflMI CCANNNNNTGG 1 cut(s) 790
PinAI ACCGGT 1 cut(s) 432
PkrI GCNGC 5 cut(s) 703, 1015, 1140, 1147, 1192
PleI GAGTC 4 cut(s) 570, 892, 952, 1154
PpsI GAGTC 4 cut(s) 570, 892, 952, 1154
PpuMI RGGWCCY 1 cut(s) 196
PscI ACATGT 1 cut(s) 774
Psp5II RGGWCCY 1 cut(s) 196
Psp6I CCWGG 2 cut(s) 167, 1132
PspGI CCWGG 2 cut(s) 167, 1132
PspN4I GGNNCC 2 cut(s) 198, 217
PspPI GGNCC 4 cut(s) 109, 132, 196, 430
PspPPI RGGWCCY 1 cut(s) 196
PstI CTGCAG 2 cut(s) 706, 1249
PstNI CAGNNNCTG 1 cut(s) 710
PsuI RGATCY 1 cut(s) 215
RsaI GTAC 4 cut(s) 212, 308, 560, 593
RsaNI GTAC 4 cut(s) 211, 307, 559, 592
RseI CAYNNNNRTG 1 cut(s) 566
SacII CCGCGG 1 cut(s) 1027
SaqAI TTAA 5 cut(s) 426, 582, 846, 1322, 1347
SatI GCNGC 5 cut(s) 702, 1014, 1139, 1146, 1191
Sau3AI GATC 4 cut(s) 215, 526, 535, 603
Sau96I GGNCC 4 cut(s) 109, 132, 196, 430
SchI GAGTC 4 cut(s) 570, 893, 953, 1154
ScrFI CCNGG 3 cut(s) 169, 441, 1134
SfaNI GCATC 1 cut(s) 332
SfcI CTRYAG 3 cut(s) 702, 879, 1245
Sfr303I CCGCGG 1 cut(s) 1027
SgrBI CCGCGG 1 cut(s) 1027
SinI GGWCC 3 cut(s) 109, 196, 430
SmiMI CAYNNNNRTG 1 cut(s) 566
SmlI CTYRAG 2 cut(s) 425, 581
SmoI CTYRAG 2 cut(s) 425, 581
Sse9I AATT 5 cut(s) 394, 414, 855, 1037, 1362
SsiI CCGC 8 cut(s) 227, 765, 814, 842, 1024, 1026, 1146, 1188
SspI AATATT 1 cut(s) 642
SspMI CTAG 3 cut(s) 620, 1379, 1385
StyD4I CCNGG 3 cut(s) 167, 439, 1132
StyI CCWWGG 1 cut(s) 634
TaaI ACNGT 3 cut(s) 311, 880, 1292
TaqI TCGA 2 cut(s) 174, 1002
TaqII GACCGA 1 cut(s) 754
TasI AATT 5 cut(s) 394, 414, 855, 1037, 1362
TatI WGTACW 1 cut(s) 558
TauI GCSGC 1 cut(s) 1148
TfiI GAWTC 3 cut(s) 751, 931, 950
Tru1I TTAA 5 cut(s) 426, 582, 846, 1322, 1347
Tru9I TTAA 5 cut(s) 426, 582, 846, 1322, 1347
TscAI CASTG 5 cut(s) 316, 333, 568, 816, 1342
TseI GCWGC 4 cut(s) 701, 1013, 1138, 1190
TspDTI ATGAA 5 cut(s) 51, 640, 764, 1111, 1293
TspGWI ACGGA 1 cut(s) 228
TspRI CASTG 5 cut(s) 316, 333, 568, 816, 1342
Van91I CCANNNNNTGG 1 cut(s) 790
Vha464I CTTAAG 2 cut(s) 425, 581
VpaK11BI GGWCC 3 cut(s) 109, 196, 430
XapI RAATTY 2 cut(s) 394, 1037
XceI RCATGY 1 cut(s) 778
XmnI GAANNNNTTC 1 cut(s) 1262
XspI CTAG 3 cut(s) 620, 1379, 1385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.