MD17G1236900.v1.1

DNA excision repair protein ERCC-6-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0013205
Physical Location & Seq
Reverse (-)
28549255 .. 28562717
13463 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1236900.v1.1.491

Sequence Viewer

Length: 2661 bp
ATGTCCCTCCAGTCCTTCAAAGAAGCCCTTAAACCCTGCAGAAACTCATCATCCTTAACAATCCCATCAACCCAATCACAGTCCTCAATCTCCCAACCCGTAGAAGAACCTACAATCCCAAGACGTCCCCCCAAATCGTCTCTGTCTCAGCAGCTTCTGAGACTGCAAGACCCATTGTCTCTGCCGCCAATTCAAACCAAACAAATCCAGGACCAGAATGGTAGTGAGGATGAGGTTGATGAAGAAGAAGACGACCCAGAATCAGTGGCCTGTGGGAGCCCTAAAGTGGGTTTCTTTGAATTTGATCACACAGGACCATATGAGCCCCTGATCTTGTCCTGTGAGGGAGAATTTCCAGTTGTACAGGTTCCTGCATCCATTAACTGCAGGTTGCTTGAACATCAGAGAGAGGGAGTGAAGTTTCTGTATACTCTGTACAAGAAAAATCATGGAGGTATTCTTGGTGACGATATGGGACTAGGAAAAACCATCCAAACAATTGCATTCTTGGCTGCTGTGTTTGGGAAAGATGGGGATTGCATCGACTCCACAGTACTGAAGAAATGCCAAACAGCTGAAAGAGCCCCTGTTCTCATAGTTTGTCCTACTTCAGTTATCCACAATTGGGAGAATGAATTCTCTAAGTGGTCTAACTTTAGTGTTGCTGTTTACCACGGTGCAAATCGTGATTTGATTTATGATAAATTAGGCTCACATGAAGTTGAGATACTTATCACAAGCTTTGACACATACAGAATATGTGGCAGCCAGTTGTCAGAGGTGAATTGGGAGATCGTGATTGTTGATGAAGCACATCGGCTTAAAAATGAAAAATCAAAGCTCTATATAGCATGTTTAGAATTTAAAACCTTAAAGCGTATTGGTCTCACTGGAACCATAATGCAGAACAAAATCATGGAACTTTTTAACCTCTTTGACTGGGTTGCACCTGGATCCTTGGGAACACGTGAACATTTTCGGGAGTTTTATGATGAACCCCTCAAGCATGGCCAAAGGTCAACTGCTCCTGAAAGATTTGTTCGTGTTGCTGATAAGCGAAAACAACACCTAGTGGCAGTTCTTCATAAATATATGTTAAGAAGGACGAAAGAGGAAACTATTGGGCATCTTATGATGGGGAAGGAAGATAATGTCATATTCTGTGCTATGAGTGAATTGCAAAAACGGGTGTATAGGAGAATGTTACAGCTACCGGATATCCAATGCCTAATAAATAAGGACAACCCATGTAGTTGTGGAAGCCCTCTTACCCAAGCTGAGTGCTGTAAAAGGACTATCCCGCATGGAAAGCTTTGGCCATACTTTCACAAGGAGAACCCCGACGGTTGTGATTCATGCCCCTTCTGCATTGTCCTTCCTTGCCTTGTCAAGCTCCAACAGATAAGTAATCACTTGGAGCTGATTAAGCCTAATCCAAAGGATGACCCAGATAAGCAAAAGAAAGATGCAGAGTTTGCCACCGCTGTGTTTGGCGAAGATGCTGAATTGGTGGGAGGGAACACCCAAAATGAGAGTTTTATGGGCCTGAGTGATGTTAAACATTGTGGCAAAATGCGAGCACTTGAAAAGTTCTTGTTCTCATGGATTTCACGGGGTGACAAAGTTCTTTTGTTCAGTTACTCTGTCAGGATGCTGGACATACTGGAAAAGTTTCTCATACGCAAAGGCTATTGCTTCTCAAGACTTGATGGTTCAACACCAACCAACTTGCGTCAGTCTCTTGTTGATGACTTTAATTCAAGTCCTAGCAAACAGGTGTTCCTTATATCAACTCGAGCTGGTGGGCTTGGGTTGAATCTGGTCAGCGCAAATCGTGTGGTAATATTTGATCCAAGTTGGAACCCTGCACAAGACTTACAGGCCCAGGACCGGTCATTTCGTTTCGGACAGAAGAGGCATGTTGTGGTTTTCCGCTTTCTTTCTGCTGGTTCCCTTGATGAGCTTGTTTATTCACGTCAGGTGTACAAACAGCAGCTATCCAACATTGCTGTTTCTGGAAAAATGGAGAAAAGATATTTTGAAGGTGTCCAGGATTGCAAAGAATTTCAAGGGGAACTTTTCGGAATCTGCAATTTATTTCGTGACCTTTCAGATAAGCTATTTACCAGCGAAATCTTTGAGCTGAATGAGAAACAAACTCAAAAAGAATTGTATAGTACAAAACAAGAATCAACCAAAGTTGGTAGCGATCATGTTTCATTAAAAGAAGTGGATGTCACCTCTTCATCAGTGTCTGAGGCCAGAACAACCAGTGATTCTGAGAAGCGTTTAACAAGTCAACCTGTGCTCAAAGAAGTCGGTATCGTGTATGCACATCGTAATGAAGACATTGTGAATTATGGACCTGGAACTCAAGGGACAACAGAAATGACCATCCCTCAGAATGGTAGCCTCGTGGAACCAGGCATTCATGTCTCGAGGAAGAAGAAACTAGATGGTATCGGTGGGAAGGAAAATTTTCCTTCACCCATGGATCAGAAAAGGATCCGGTACAGCCGCCTTTCTAAGTTCATGGGTTTGGGAGAGCTTGAATTTAGCAAGTGGGTAATGTCTGCCACACCAATGGAGAGGGAGAGAGTGCTCGGGGACTTCAGGAAGAGAAAGAAGAAGACGCTTGAAAACATTATTTTCGAAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helicase_C SNF2_N

Protein Analysis

887

Amino Acids

100.49

Weight (kDa)

7.48

Isoelectric Point (pI)

43.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 130 - 300 4.3e-09 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 134 - 408 1.2e-66 SNF2-related domain
DEAD PF00270 154 - 301 1.2e-06 DEAD/DEAH box helicase
Helicase_C PF00271 524 - 636 7.1e-24 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013205)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03750
fragaria_vesca FvH4_6g26480 FvH4_6g26480 FvH4_6g26480 FvH4_6g26480
malus_domestica MD17G1236900.v1.1
prunus_persica Prupe.3G139100_v2.0.a1
pyrus_communis pycom17g24160
rosa_chinensis RchiOBHm_Chr2g0127761
rosa_laevigata RLG00000018935
rosa_multiflora Rmu_sc0001829.1_g000008 Rmu_sc0004240.1_g000009
rosa_roxburghii Rroxscaffold_2G00116780
rosa_rugosa Rorug02G0271200
rosa_samantha Rh2AG323300 Rh2BG332500 Rh2CG311100
rosa_wichuraiana Rw2G026240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 127
Acc36I ACCTGC 1 cut(s) 378
AccI GTMKAC 1 cut(s) 428
AciI CCGC 5 cut(s) 185, 1301, 1482, 1933, 2518
AclWI GGATC 6 cut(s) 948, 961, 1844, 2500, 2502, 2513
AcoI YGGCCR 2 cut(s) 1009, 1316
AcsI RAATTY 7 cut(s) 299, 350, 635, 860, 2063, 2476, 2552
AcuI CTGAAG 3 cut(s) 578, 594, 2596
AcvI CACGTG 1 cut(s) 968
AcyI GRCGYC 1 cut(s) 124
AdeI CACNNNGTG 2 cut(s) 1072, 1616
AfaI GTAC 6 cut(s) 363, 437, 555, 1985, 2179, 2513
AfiI CCNNNNNNNGG 5 cut(s) 286, 287, 625, 1890, 2405
AflIII ACRYGT 1 cut(s) 965
AgeI ACCGGT 1 cut(s) 1890
AhdI GACNNNNNGTC 1 cut(s) 175
AjiI CACGTC 1 cut(s) 1976
AjnI CCWGG 6 cut(s) 207, 949, 1884, 2049, 2365, 2422
AleI CACNNNNGTG 1 cut(s) 1484
AloI GAACNNNNNNTCC 2 cut(s) 99, 131
Alw21I GWGCWC 3 cut(s) 1582, 2310, 2604
Alw26I GTCTC 7 cut(s) 144, 150, 154, 183, 890, 1743, 2440
AlwI GGATC 6 cut(s) 948, 961, 1844, 2500, 2502, 2513
AlwNI CAGNNNCTG 2 cut(s) 157, 2255
Ama87I CYCGRG 3 cut(s) 1794, 2437, 2603
AoxI GGCC 6 cut(s) 267, 1009, 1316, 1543, 1881, 2259
ApeKI GCWGC 4 cut(s) 151, 512, 765, 1993
ApoI RAATTY 7 cut(s) 299, 350, 635, 860, 2063, 2476, 2552
AsiGI ACCGGT 1 cut(s) 1890
Asp700I GAANNNNTTC 4 cut(s) 635, 975, 1671, 2478
AspLEI GCGC 1 cut(s) 1829
AspS9I GGNCC 6 cut(s) 211, 314, 1543, 1882, 1888, 2363
AsuHPI GGTGA 5 cut(s) 476, 793, 1628, 2230, 2478
AsuII TTCGAA 1 cut(s) 2652
AvaI CYCGRG 3 cut(s) 1794, 2437, 2603
AvaII GGWCC 4 cut(s) 211, 314, 1888, 2363
BalI TGGCCA 2 cut(s) 1011, 1318
BamHI GGATCC 2 cut(s) 953, 2505
BanII GRGCYC 3 cut(s) 281, 327, 586
BarI GAAGNNNNNNTAC 4 cut(s) 711, 743, 1252, 1284
BauI CACGAG 1 cut(s) 2414
BbrPI CACGTG 1 cut(s) 968
BbsI GAAGAC 3 cut(s) 255, 2352, 2636
Bbv12I GWGCWC 3 cut(s) 1582, 2310, 2604
BbvI GCAGC 4 cut(s) 163, 499, 777, 2005
BccI CCATC 7 cut(s) 73, 497, 524, 1129, 1703, 2402, 2450
BciT130I CCWGG 6 cut(s) 209, 951, 1886, 2051, 2367, 2424
BclI TGATCA 1 cut(s) 304
BcoDI GTCTC 7 cut(s) 144, 150, 154, 183, 890, 1743, 2440
BfaI CTAG 4 cut(s) 479, 1070, 1767, 2453
BfmI CTRYAG 2 cut(s) 37, 385
BfuAI ACCTGC 1 cut(s) 378
BisI GCNGC 6 cut(s) 152, 185, 513, 766, 1994, 2518
BlsI GCNGC 6 cut(s) 153, 186, 514, 767, 1995, 2519
BmcAI AGTACT 1 cut(s) 555
Bme1390I CCNGG 6 cut(s) 209, 951, 1886, 2051, 2367, 2424
Bme18I GGWCC 4 cut(s) 211, 314, 1888, 2363
BmeRI GACNNNNNGTC 1 cut(s) 175
BmeT110I CYCGRG 3 cut(s) 1794, 2437, 2603
BmgBI CACGTC 1 cut(s) 1976
BmgT120I GGNCC 6 cut(s) 211, 314, 1543, 1882, 1888, 2363
BmiI GGNNCC 8 cut(s) 278, 369, 895, 955, 1862, 1951, 2421, 2507
BmrFI CCNGG 6 cut(s) 209, 951, 1886, 2051, 2367, 2424
BmrI ACTGGG 1 cut(s) 949
BmsI GCATC 6 cut(s) 383, 549, 1135, 1456, 1489, 1641
BmuI ACTGGG 1 cut(s) 949
BpiI GAAGAC 3 cut(s) 255, 2352, 2636
Bpu14I TTCGAA 1 cut(s) 2652
BpuEI CTTGAG 3 cut(s) 986, 1684, 2358
BsaAI YACGTR 1 cut(s) 968
BsaBI GATNNNNATC 1 cut(s) 731
BsaHI GRCGYC 1 cut(s) 124
BsaI GGTCTC 1 cut(s) 890
BsaJI CCNNGG 4 cut(s) 673, 957, 1884, 2490
BsaWI WCCGGW 3 cut(s) 1213, 1890, 2508
Bsc4I CCNNNNNNNGG 5 cut(s) 286, 287, 625, 1890, 2405
Bse118I RCCGGY 1 cut(s) 1890
Bse1I ACTGG 7 cut(s) 10, 356, 769, 895, 944, 1668, 2271
Bse3DI GCAATG 1 cut(s) 2004
Bse8I GATNNNNATC 1 cut(s) 731
BseBI CCWGG 6 cut(s) 209, 951, 1886, 2051, 2367, 2424
BseDI CCNNGG 4 cut(s) 673, 957, 1884, 2490
BseGI GGATG 8 cut(s) 50, 235, 374, 489, 1447, 1656, 2239, 2394
BseJI GATNNNNATC 1 cut(s) 731
BseLI CCNNNNNNNGG 5 cut(s) 286, 287, 625, 1890, 2405
BseMI GCAATG 1 cut(s) 2004
BseMII CTCAG 7 cut(s) 149, 161, 1269, 1538, 2247, 2271, 2414
BseNI ACTGG 7 cut(s) 10, 356, 769, 895, 944, 1668, 2271
BseXI GCAGC 4 cut(s) 163, 499, 777, 2005
BsgI GTGCAG 1 cut(s) 1851
BshFI GGCC 6 cut(s) 269, 1011, 1318, 1545, 1883, 2261
BshTI ACCGGT 1 cut(s) 1890
BsiHKAI GWGCWC 3 cut(s) 1582, 2310, 2604
BsiHKCI CYCGRG 3 cut(s) 1794, 2437, 2603
BsiSI CCGG 3 cut(s) 1214, 1891, 2509
BslFI GGGAC 4 cut(s) 111, 489, 2392, 2621
BslI CCNNNNNNNGG 5 cut(s) 286, 287, 625, 1890, 2405
BsmAI GTCTC 7 cut(s) 144, 150, 154, 183, 890, 1743, 2440
BsmBI CGTCTC 1 cut(s) 144
BsmFI GGGAC 4 cut(s) 111, 489, 2392, 2621
BsmI GAATGC 2 cut(s) 503, 2427
BsnI GGCC 6 cut(s) 269, 1011, 1318, 1545, 1883, 2261
Bso31I GGTCTC 1 cut(s) 890
BsoBI CYCGRG 3 cut(s) 1794, 2437, 2603
Bsp119I TTCGAA 1 cut(s) 2652
Bsp1286I GDGCHC 6 cut(s) 281, 327, 586, 1582, 2310, 2604
Bsp1407I TGTACA 3 cut(s) 361, 435, 1983
Bsp143I GATC 8 cut(s) 304, 330, 792, 953, 1849, 2209, 2494, 2505
Bsp19I CCATGG 1 cut(s) 2490
BspACI CCGC 5 cut(s) 185, 1301, 1482, 1933, 2518
BspANI GGCC 6 cut(s) 269, 1011, 1318, 1545, 1883, 2261
BspCNI CTCAG 7 cut(s) 150, 160, 1270, 1539, 2248, 2272, 2413
BspLI GGNNCC 8 cut(s) 278, 369, 895, 955, 1862, 1951, 2421, 2507
BspMAI CTGCAG 2 cut(s) 41, 389
BspMI ACCTGC 1 cut(s) 378
BspPI GGATC 6 cut(s) 948, 961, 1844, 2500, 2502, 2513
BspT104I TTCGAA 1 cut(s) 2652
BspTNI GGTCTC 1 cut(s) 890
BsrDI GCAATG 1 cut(s) 2004
BsrFI RCCGGY 1 cut(s) 1890
BsrGI TGTACA 3 cut(s) 361, 435, 1983
BsrI ACTGG 7 cut(s) 10, 356, 769, 895, 944, 1668, 2271
BssAI RCCGGY 1 cut(s) 1890
BssECI CCNNGG 4 cut(s) 673, 957, 1884, 2490
BssMI GATC 8 cut(s) 304, 330, 792, 953, 1849, 2209, 2494, 2505
BssNAI GTATAC 1 cut(s) 429
BssNI GRCGYC 1 cut(s) 124
BssSI CACGAG 1 cut(s) 2414
BssT1I CCWWGG 2 cut(s) 957, 2490
Bst1107I GTATAC 1 cut(s) 429
Bst2BI CACGAG 1 cut(s) 2414
Bst2UI CCWGG 6 cut(s) 209, 951, 1886, 2051, 2367, 2424
Bst4CI ACNGT 4 cut(s) 81, 553, 677, 1346
Bst6I CTCTTC 3 cut(s) 1907, 2248, 2612
BstACI GRCGYC 1 cut(s) 124
BstAPI GCANNNNNTGC 1 cut(s) 1475
BstAUI TGTACA 3 cut(s) 361, 435, 1983
BstBAI YACGTR 1 cut(s) 968
BstBI TTCGAA 1 cut(s) 2652
BstC8I GCNNGC 1 cut(s) 1578
BstDEI CTNAG 9 cut(s) 147, 158, 642, 1278, 1547, 2256, 2280, 2400, 2526
BstDSI CCRYGG 2 cut(s) 673, 2490
BstF5I GGATG 8 cut(s) 50, 235, 374, 489, 1447, 1656, 2239, 2394
BstHHI GCGC 1 cut(s) 1829
BstKTI GATC 8 cut(s) 307, 333, 795, 956, 1852, 2212, 2497, 2508
BstMAI GTCTC 7 cut(s) 144, 150, 154, 183, 890, 1743, 2440
BstMBI GATC 8 cut(s) 304, 330, 792, 953, 1849, 2209, 2494, 2505
BstMWI GCNNNNNNNGC 6 cut(s) 509, 581, 1309, 1365, 1426, 1475
BstNI CCWGG 6 cut(s) 209, 951, 1886, 2051, 2367, 2424
BstNSI RCATGY 2 cut(s) 855, 1922
BstSCI CCNGG 6 cut(s) 207, 949, 1884, 2049, 2365, 2422
BstSFI CTRYAG 2 cut(s) 37, 385
BstV1I GCAGC 4 cut(s) 163, 499, 777, 2005
BstV2I GAAGAC 3 cut(s) 255, 2352, 2636
BstX2I RGATCY 2 cut(s) 953, 2505
BstXI CCANNNNNNTGG 1 cut(s) 2584
BstYI RGATCY 2 cut(s) 953, 2505
BstZ17I GTATAC 1 cut(s) 429
BsuRI GGCC 6 cut(s) 269, 1011, 1318, 1545, 1883, 2261
BtgI CCRYGG 2 cut(s) 673, 2490
BtrI CACGTC 1 cut(s) 1976
BtsCI GGATG 8 cut(s) 50, 235, 374, 489, 1447, 1656, 2239, 2394
BtsIMutI CAGTG 4 cut(s) 270, 888, 2256, 2278
BveI ACCTGC 1 cut(s) 378
Cac8I GCNNGC 1 cut(s) 1578
CaiI CAGNNNCTG 2 cut(s) 157, 2255
CfoI GCGC 1 cut(s) 1829
Cfr10I RCCGGY 1 cut(s) 1890
Cfr13I GGNCC 6 cut(s) 211, 314, 1543, 1882, 1888, 2363
CseI GACGC 2 cut(s) 1721, 2641
Csp6I GTAC 6 cut(s) 362, 436, 554, 1984, 2178, 2512
CspAI ACCGGT 1 cut(s) 1890
CspCI CAANNNNNGTGG 2 cut(s) 1818, 1853
CviQI GTAC 6 cut(s) 362, 436, 554, 1984, 2178, 2512
DdeI CTNAG 9 cut(s) 147, 158, 642, 1278, 1547, 2256, 2280, 2400, 2526
DpnI GATC 8 cut(s) 306, 332, 794, 955, 1851, 2211, 2496, 2507
DpnII GATC 8 cut(s) 304, 330, 792, 953, 1849, 2209, 2494, 2505
DraI TTTAAA 1 cut(s) 865
DraIII CACNNNGTG 2 cut(s) 1072, 1616
DriI GACNNNNNGTC 1 cut(s) 175
EaeI YGGCCR 2 cut(s) 1009, 1316
Eam1104I CTCTTC 3 cut(s) 1907, 2248, 2612
Eam1105I GACNNNNNGTC 1 cut(s) 175
EarI CTCTTC 3 cut(s) 1907, 2248, 2612
Eco130I CCWWGG 2 cut(s) 957, 2490
Eco24I GRGCYC 3 cut(s) 281, 327, 586
Eco31I GGTCTC 1 cut(s) 890
Eco32I GATATC 1 cut(s) 1219
Eco47I GGWCC 4 cut(s) 211, 314, 1888, 2363
Eco57I CTGAAG 3 cut(s) 578, 594, 2596
Eco72I CACGTG 1 cut(s) 968
Eco88I CYCGRG 3 cut(s) 1794, 2437, 2603
EcoRI GAATTC 1 cut(s) 635
EcoRII CCWGG 6 cut(s) 207, 949, 1884, 2049, 2365, 2422
EcoRV GATATC 1 cut(s) 1219
EcoT14I CCWWGG 2 cut(s) 957, 2490
EcoT38I GRGCYC 3 cut(s) 281, 327, 586
ErhI CCWWGG 2 cut(s) 957, 2490
Esp3I CGTCTC 1 cut(s) 144
FalI AAGNNNNNCTT 4 cut(s) 12, 44, 2061, 2093
FaqI GGGAC 4 cut(s) 111, 489, 2392, 2621
FauI CCCGC 1 cut(s) 1308
FauNDI CATATG 1 cut(s) 319
FbaI TGATCA 1 cut(s) 304
FblI GTMKAC 1 cut(s) 428
Fnu4HI GCNGC 6 cut(s) 152, 185, 513, 766, 1994, 2518
FokI GGATG 8 cut(s) 37, 242, 361, 476, 1454, 1663, 2246, 2381
FriOI GRGCYC 3 cut(s) 281, 327, 586
Fsp4HI GCNGC 6 cut(s) 152, 185, 513, 766, 1994, 2518
FspBI CTAG 4 cut(s) 479, 1070, 1767, 2453
GlaI GCGC 1 cut(s) 1828
GluI GCNGC 6 cut(s) 152, 185, 513, 766, 1994, 2518
HaeIII GGCC 6 cut(s) 269, 1011, 1318, 1545, 1883, 2261
HapII CCGG 3 cut(s) 1214, 1891, 2509
HgaI GACGC 2 cut(s) 1721, 2641
HhaI GCGC 1 cut(s) 1829
Hin1I GRCGYC 1 cut(s) 124
Hin6I GCGC 1 cut(s) 1827
HinP1I GCGC 1 cut(s) 1827
HincII GTYRAC 2 cut(s) 1020, 2300
HindII GTYRAC 2 cut(s) 1020, 2300
HindIII AAGCTT 2 cut(s) 739, 1310
HinfI GANTC 7 cut(s) 260, 545, 1352, 1816, 2085, 2189, 2276
HpaII CCGG 3 cut(s) 1214, 1891, 2509
HphI GGTGA 5 cut(s) 476, 793, 1628, 2230, 2478
Hpy166II GTNNAC 6 cut(s) 429, 670, 971, 1020, 1984, 2300
Hpy8I GTNNAC 6 cut(s) 429, 670, 971, 1020, 1984, 2300
Hpy99I CGWCG 1 cut(s) 1346
HpyAV CCTTC 8 cut(s) 25, 1095, 1135, 1372, 1385, 2036, 2464, 2493
HpyCH4III ACNGT 4 cut(s) 81, 553, 677, 1346
HpyCH4IV ACGT 3 cut(s) 124, 967, 1975
HpyF10VI GCNNNNNNNGC 6 cut(s) 509, 581, 1309, 1365, 1426, 1475
HpyF3I CTNAG 9 cut(s) 147, 158, 642, 1278, 1547, 2256, 2280, 2400, 2526
HpySE526I ACGT 3 cut(s) 124, 967, 1975
Hsp92I GRCGYC 1 cut(s) 124
HspAI GCGC 1 cut(s) 1827
Ksp22I TGATCA 1 cut(s) 304
Kzo9I GATC 8 cut(s) 304, 330, 792, 953, 1849, 2209, 2494, 2505
LmnI GCTCC 4 cut(s) 276, 1030, 1398, 1417
Lsp1109I GCAGC 4 cut(s) 163, 499, 777, 2005
LweI GCATC 6 cut(s) 383, 549, 1135, 1456, 1489, 1641
MaeI CTAG 4 cut(s) 479, 1070, 1767, 2453
MaeII ACGT 3 cut(s) 124, 967, 1975
MaeIII GTNAC 6 cut(s) 464, 1203, 1616, 1637, 2102, 2236
MalI GATC 8 cut(s) 306, 332, 794, 955, 1851, 2211, 2496, 2507
MboI GATC 8 cut(s) 304, 330, 792, 953, 1849, 2209, 2494, 2505
MfeI CAATTG 2 cut(s) 498, 622
MflI RGATCY 2 cut(s) 953, 2505
MhlI GDGCHC 6 cut(s) 281, 327, 586, 1582, 2310, 2604
MlsI TGGCCA 2 cut(s) 1011, 1318
MluNI TGGCCA 2 cut(s) 1011, 1318
MlyI GAGTC 1 cut(s) 539
MmeI TCCRAC 3 cut(s) 1420, 1838, 2025
Mox20I TGGCCA 2 cut(s) 1011, 1318
MroXI GAANNNNTTC 4 cut(s) 635, 975, 1671, 2478
MscI TGGCCA 2 cut(s) 1011, 1318
MslI CAYNNNNRTG 3 cut(s) 1484, 2340, 2582
Msp20I TGGCCA 2 cut(s) 1011, 1318
MspA1I CMGCKG 2 cut(s) 575, 1484
MspI CCGG 3 cut(s) 1214, 1891, 2509
MspR9I CCNGG 6 cut(s) 209, 951, 1886, 2051, 2367, 2424
MunI CAATTG 2 cut(s) 498, 622
Mva1269I GAATGC 2 cut(s) 503, 2427
MvaI CCWGG 6 cut(s) 209, 951, 1886, 2051, 2367, 2424
MwoI GCNNNNNNNGC 6 cut(s) 509, 581, 1309, 1365, 1426, 1475
NcoI CCATGG 1 cut(s) 2490
NdeI CATATG 1 cut(s) 319
NdeII GATC 8 cut(s) 304, 330, 792, 953, 1849, 2209, 2494, 2505
NlaIV GGNNCC 8 cut(s) 278, 369, 895, 955, 1862, 1951, 2421, 2507
NmuCI GTSAC 4 cut(s) 464, 1616, 2102, 2236
NspI RCATGY 2 cut(s) 855, 1922
NspV TTCGAA 1 cut(s) 2652
OliI CACNNNNGTG 1 cut(s) 1484
PaeR7I CTCGAG 2 cut(s) 1794, 2437
PctI GAATGC 2 cut(s) 503, 2427
PdmI GAANNNNTTC 4 cut(s) 635, 975, 1671, 2478
PfeI GAWTC 6 cut(s) 260, 1352, 1816, 2085, 2189, 2276
PfoI TCCNGGA 2 cut(s) 207, 2049
PinAI ACCGGT 1 cut(s) 1890
PkrI GCNGC 6 cut(s) 153, 186, 514, 767, 1995, 2519
PleI GAGTC 1 cut(s) 539
PmaCI CACGTG 1 cut(s) 968
PmlI CACGTG 1 cut(s) 968
PpsI GAGTC 1 cut(s) 539
Ppu21I YACGTR 1 cut(s) 968
Psp6I CCWGG 6 cut(s) 207, 949, 1884, 2049, 2365, 2422
PspCI CACGTG 1 cut(s) 968
PspGI CCWGG 6 cut(s) 207, 949, 1884, 2049, 2365, 2422
PspN4I GGNNCC 8 cut(s) 278, 369, 895, 955, 1862, 1951, 2421, 2507
PspPI GGNCC 6 cut(s) 211, 314, 1543, 1882, 1888, 2363
PspXI VCTCGAGB 1 cut(s) 1794
PstI CTGCAG 2 cut(s) 41, 389
PstNI CAGNNNCTG 2 cut(s) 157, 2255
PsuI RGATCY 2 cut(s) 953, 2505
PvuII CAGCTG 1 cut(s) 575
RsaI GTAC 6 cut(s) 363, 437, 555, 1985, 2179, 2513
RsaNI GTAC 6 cut(s) 362, 436, 554, 1984, 2178, 2512
RseI CAYNNNNRTG 3 cut(s) 1484, 2340, 2582
SatI GCNGC 6 cut(s) 152, 185, 513, 766, 1994, 2518
Sau3AI GATC 8 cut(s) 304, 330, 792, 953, 1849, 2209, 2494, 2505
Sau96I GGNCC 6 cut(s) 211, 314, 1543, 1882, 1888, 2363
ScaI AGTACT 1 cut(s) 555
SchI GAGTC 1 cut(s) 539
ScrFI CCNGG 6 cut(s) 209, 951, 1886, 2051, 2367, 2424
SduI GDGCHC 6 cut(s) 281, 327, 586, 1582, 2310, 2604
SfaNI GCATC 6 cut(s) 383, 549, 1135, 1456, 1489, 1641
SfcI CTRYAG 2 cut(s) 37, 385
Sfr274I CTCGAG 2 cut(s) 1794, 2437
SfuI TTCGAA 1 cut(s) 2652
SinI GGWCC 4 cut(s) 211, 314, 1888, 2363
SlaI CTCGAG 2 cut(s) 1794, 2437
SmiMI CAYNNNNRTG 3 cut(s) 1484, 2340, 2582
SmlI CTYRAG 5 cut(s) 1001, 1699, 1794, 2373, 2437
SmoI CTYRAG 5 cut(s) 1001, 1699, 1794, 2373, 2437
SsiI CCGC 5 cut(s) 185, 1301, 1482, 1933, 2518
SspI AATATT 1 cut(s) 1845
SspMI CTAG 4 cut(s) 479, 1070, 1767, 2453
StyD4I CCNGG 6 cut(s) 207, 949, 1884, 2049, 2365, 2422
StyI CCWWGG 2 cut(s) 957, 2490
TaaI ACNGT 4 cut(s) 81, 553, 677, 1346
TaiI ACGT 3 cut(s) 127, 970, 1978
TaqI TCGA 4 cut(s) 543, 1795, 2438, 2652
TatI WGTACW 5 cut(s) 361, 435, 553, 1983, 2177
TauI GCSGC 2 cut(s) 187, 2520
TfiI GAWTC 6 cut(s) 260, 1352, 1816, 2085, 2189, 2276
TscAI CASTG 4 cut(s) 270, 895, 2256, 2278
TseFI GTSAC 4 cut(s) 464, 1616, 2102, 2236
TseI GCWGC 4 cut(s) 151, 512, 765, 1993
Tsp45I GTSAC 4 cut(s) 464, 1616, 2102, 2236
TspRI CASTG 4 cut(s) 270, 895, 2256, 2278
VpaK11BI GGWCC 4 cut(s) 211, 314, 1888, 2363
XapI RAATTY 7 cut(s) 299, 350, 635, 860, 2063, 2476, 2552
XceI RCATGY 2 cut(s) 855, 1922
XcmI CCANNNNNNNNNTGG 1 cut(s) 215
XhoI CTCGAG 2 cut(s) 1794, 2437
XmiI GTMKAC 1 cut(s) 428
XmnI GAANNNNTTC 4 cut(s) 635, 975, 1671, 2478
XspI CTAG 4 cut(s) 479, 1070, 1767, 2453
ZraI GACGTC 1 cut(s) 125
ZrmI AGTACT 1 cut(s) 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.