Rroxscaffold_2G00116780

DNA excision repair protein ERCC-6-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0013205
Physical Location & Seq
Reverse (-)
44019460 .. 44025697
6238 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00116780.1

Sequence Viewer

Length: 2643 bp
ATGTCTCTCCGCGCCTTCAAAGAAAGCCTTAAACCCTGCCAAACCCAAAACTCCTCGTCGACCCAAACACGGTCGTCGTCAATTCTGTCCCAAATCCCAGAACCCACAATCCCTAGAAAGCCCCCCAAATCGTCTCTGGCTCAGCAGCTCTTGAGGCTCCAAGACCCTCTGTCTCTTCAACCACCTCCACCAACGCCCCTAGAGAAGACCCAAATCAAACAAACCCAGGTGGAGGATCCCGATGAGGAGGACGAAGACGACCCAGAACCATTGGGATGCTCTGGGATTAGTAAAGTTAGTAGCTTTCAGTTTGATCACACAGGACCCTATGAACCCTTGGTCTTATCCGATTTTCCTCTCATTCAGGTTCCTGCATCCATTAACTGCAGATTGCTTCCACATCAGAGGGAGGGAGTAAAGTTTCTATACAATATATACAAGAACAACCATGGAGGGGTTCTTGGAGATGACATGGGGCTAGGCAAAACCATTCAGACAATTGCATTCTTGGCTGCTGTGTTTGGGAAAGATGGTGATCGCATTGATTCCACAATTGCAAAGAGAAGTGAAATAGCAGAACGAAACCCTGTTCTCATAGTTTGCCCCAGTTCAGTTATCCACAACTGGGAGAGCGAATTCTCTAAGTGGTCTAACTTTAGTGTTGCTGTTTACCATGGTGCAAATCGGGATTTGATTTATGATAAATTGGAAGCTCATGGAGTTGAGATACTTATCACGAGCTTTGACACATACAGAATTTGTGGCAGCCAGTTATCAGATGTTAATTGGGAGATTGTTGTTGTCGATGAGGCACACCGGCTTAAGAATGAAAAATCAAAGCTCTACATAGCATGTCTAGAATTTAAAACCTTAAAACGAATTGGTCTCACTGGAACTATGATGCAGAACAAAATCATGGAATTGTTTAACCTGTTTGACTGGGTTGCACCTGGATCCTTGGGAACACGGGAGCATTTTCGGGAGTTTTATGATGAACCTCTCAAACATGGCCAAAGATCAACTGCTCCGGAAAGATTTGTCCGCATTGCTGATGAGCGAAAACAGCATCTAGTAGCTGTTCTTAATAAATATATGTTGAGAAGGACAAAAGAGGAAACCATTGGTCATCTTATGATGGGGAAGGAAGACAATGTCATATTTTGTTCAATGAGCGAATTGCAAAAGCGGGTGTATAGGCGAATGTTACAGCTACCAGACATCCAATGCCTGATAAATAAGGACCTTCCCTGTAGTTGTGGATCTCCTCTTACCCAAGTTGAGTGCTGCAAAAGGATTGTACCAGATGGAAAAATCTGGCCATACCTTCACAGGGATAACCCTGATGGTTGTGATTCATGCCCCTTTTGTATTGTCCTTCCTTGCCTTGTCAAGCTCCAACAGATAAGTAACCACTTGGAGCTTATTAAGCCTAATCCGAAGGATGACGCAGAAAAGCAAAAGAAAGATGCAGAGTTTGCCTCTGCTGTGTTTGGCCCAGATATTGATTTGGTGGGAGGCAACACTCAAAATGAGAGTTTTATGGGCTTAAGTGATGTTAAACAGTGCGGGAAAATGCGAGCACTTGAAAAGTTTATGTTCTCATGGATTGCACAGGGTGATAAAGTCCTTCTGTTCAGTTACTCTGTCAGGATGCTGGACATATTGGAAAAATTTCTTATACGCAAAGGTTATTGCTTCTCAAGACTTGATGGTTCCACACCAACCAACTTGCGCCAGTCTCTTGTTGACAACTTTAATTCAAGTCCAAGCAAACAGGTATTCCTTATATCAACTCGAGCCGGTGGGCTTGGACTGAATCTTGTCAGTGCAAATCGTGTTGTAATATTTGATCCAAGCTGGAATCCTGCCCAAGACTTACAGGCCCAAGACAGGTCATTTCGGTTCGGGCAGAAACGGCATGTTGTGGTTTTCCGCCTTCTTTCTGCTGGTTCCCTTGATGAACTTGTTTATTCACGTCAGGTGTACAAACAGCAGCTATCAAACATTGCTGTTTCTGGAAAAATGGAGAGACGATATTTTGAAGGTGTCCAGGATTGCAAAGACTTTCAAGGGGAACTTTTTGGAATTTGCAATTTATTTCGTGATCTTTCAGATAAGCTCTTTACCAGCGAGATATTTGAGTTGCATGAAAAGCAGGGACAAAGAGAAGGGGATTGTCATGGTAGGGAACAAGAATCAACCAAAATTACTTGCGTAAAAGAAGTGGGTGTGACATCTTCATCCATGTCTGAGACCACGGATTCTGAGAAGGCTTTAGCTAGTCAACCTGTGCTTAAAGATGCGGGTATTGTCTATGCACATCGTAATGAAGACATTGTGAATAATAGACCTGGAATGCAAGGGACGATGGAAAGAGTCATTCCTTGTAATGATAGCCTAAAACAGCCCCCCACTGGTGTTGCTAGGAGAAAGCGACGAGAGGGTACTGGTGGAAAGGAGAATGTATCTGTATCCATGGATCGCAAAAGGATTCAATTTAGTCTCCTTGCTAAGTTCATGGGTATGGGAGAGATTGAATTTAGCAAGTGGGTAGTATCTGCTCCTCCTCTAGAGAGGGAAAAGGTTATCCAGGACTACAAGGAGAGAAACAAGAAGAAACTCAACAGCGTTGATTTGTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helicase_C SNF2_N

Protein Analysis

880

Amino Acids

99.62

Weight (kDa)

8.05

Isoelectric Point (pI)

43.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 129 - 300 8e-09 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 134 - 408 1.9e-65 SNF2-related domain
DEAD PF00270 153 - 303 5.4e-06 DEAD/DEAH box helicase
Helicase_C PF00271 524 - 636 2.4e-23 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013205)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03750
fragaria_vesca FvH4_6g26480 FvH4_6g26480 FvH4_6g26480 FvH4_6g26480
malus_domestica MD17G1236900.v1.1
prunus_persica Prupe.3G139100_v2.0.a1
pyrus_communis pycom17g24160
rosa_chinensis RchiOBHm_Chr2g0127761
rosa_laevigata RLG00000018935
rosa_multiflora Rmu_sc0001829.1_g000008 Rmu_sc0004240.1_g000009
rosa_roxburghii Rroxscaffold_2G00116780
rosa_rugosa Rorug02G0271200
rosa_samantha Rh2AG323300 Rh2BG332500 Rh2CG311100
rosa_wichuraiana Rw2G026240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2641
AccI GTMKAC 1 cut(s) 59
AccII CGCG 1 cut(s) 12
AccIII TCCGGA 1 cut(s) 1027
AciI CCGC 6 cut(s) 10, 1042, 1186, 1566, 1933, 2303
AclWI GGATC 7 cut(s) 230, 243, 948, 961, 1267, 1844, 2487
AcoI YGGCCR 2 cut(s) 1009, 1316
AcsI RAATTY 6 cut(s) 635, 756, 860, 1670, 2085, 2537
AdeI CACNNNGTG 1 cut(s) 1616
AfaI GTAC 3 cut(s) 1299, 1985, 2446
AfiI CCNNNNNNNGG 7 cut(s) 69, 232, 454, 625, 1330, 1890, 2414
AflII CTTAAG 2 cut(s) 821, 1546
AgsI TTSAA 9 cut(s) 19, 179, 1167, 1586, 1761, 2042, 2069, 2495, 2537
AhdI GACNNNNNGTC 1 cut(s) 169
AjiI CACGTC 1 cut(s) 1976
AjnI CCWGG 5 cut(s) 225, 949, 2049, 2350, 2589
AloI GAACNNNNNNTCC 2 cut(s) 93, 125
Alw21I GWGCWC 1 cut(s) 1582
Alw26I GTCTC 8 cut(s) 9, 138, 177, 890, 1743, 2023, 2246, 2507
AlwI GGATC 7 cut(s) 230, 243, 948, 961, 1267, 1844, 2487
Ama87I CYCGRG 1 cut(s) 1794
Aor13HI TCCGGA 1 cut(s) 1027
AoxI GGCC 4 cut(s) 1009, 1316, 1492, 1881
ApeKI GCWGC 5 cut(s) 145, 512, 765, 1284, 1993
ApoI RAATTY 6 cut(s) 635, 756, 860, 1670, 2085, 2537
Asp700I GAANNNNTTC 1 cut(s) 1671
AspLEI GCGC 2 cut(s) 14, 1734
AspS9I GGNCC 4 cut(s) 323, 1240, 1493, 1882
AsuHPI GGTGA 2 cut(s) 545, 1628
AvaI CYCGRG 1 cut(s) 1794
AvaII GGWCC 2 cut(s) 323, 1240
BalI TGGCCA 2 cut(s) 1011, 1318
BamHI GGATCC 2 cut(s) 235, 953
BauI CACGAG 1 cut(s) 736
BbsI GAAGAC 4 cut(s) 212, 261, 1152, 2337
Bbv12I GWGCWC 1 cut(s) 1582
BbvI GCAGC 5 cut(s) 157, 499, 777, 1271, 2005
BccI CCATC 6 cut(s) 524, 1129, 1298, 1337, 1703, 2362
BceAI ACGGC 1 cut(s) 1931
BciT130I CCWGG 5 cut(s) 227, 951, 2051, 2352, 2591
BciVI GTATCC 1 cut(s) 2482
BclI TGATCA 1 cut(s) 313
BcoDI GTCTC 8 cut(s) 9, 138, 177, 890, 1743, 2023, 2246, 2507
BfaI CTAG 8 cut(s) 114, 200, 479, 857, 1070, 2280, 2424, 2570
BfmI CTRYAG 2 cut(s) 385, 1249
BfrI CTTAAG 2 cut(s) 821, 1546
BfuI GTATCC 1 cut(s) 2482
BisI GCNGC 5 cut(s) 146, 513, 766, 1285, 1994
BlpI GCTNAGC 1 cut(s) 141
BlsI GCNGC 5 cut(s) 147, 514, 767, 1286, 1995
Bme1390I CCNGG 5 cut(s) 227, 951, 2051, 2352, 2591
Bme18I GGWCC 2 cut(s) 323, 1240
BmeRI GACNNNNNGTC 1 cut(s) 169
BmeT110I CYCGRG 1 cut(s) 1794
BmgBI CACGTC 1 cut(s) 1976
BmgT120I GGNCC 4 cut(s) 323, 1240, 1493, 1882
BmiI GGNNCC 7 cut(s) 158, 237, 325, 369, 955, 1714, 1951
BmrFI CCNGG 5 cut(s) 227, 951, 2051, 2352, 2591
BmrI ACTGGG 3 cut(s) 600, 634, 949
BmsI GCATC 7 cut(s) 266, 383, 891, 1075, 1456, 1641, 2290
BmuI ACTGGG 3 cut(s) 600, 634, 949
BpiI GAAGAC 4 cut(s) 212, 261, 1152, 2337
BplI GAGNNNNNCTC 2 cut(s) 1463, 1495
Bpu1102I GCTNAGC 1 cut(s) 141
BpuEI CTTGAG 2 cut(s) 172, 1684
BsaBI GATNNNNATC 2 cut(s) 534, 731
BsaI GGTCTC 2 cut(s) 890, 2246
BsaJI CCNNGG 7 cut(s) 225, 336, 448, 673, 957, 2256, 2475
BsaWI WCCGGW 1 cut(s) 1027
Bsc4I CCNNNNNNNGG 7 cut(s) 69, 232, 454, 625, 1330, 1890, 2414
Bse118I RCCGGY 2 cut(s) 816, 1799
Bse1I ACTGG 8 cut(s) 606, 629, 769, 895, 944, 1735, 2419, 2452
Bse3DI GCAATG 2 cut(s) 1044, 2004
Bse8I GATNNNNATC 2 cut(s) 534, 731
BseAI TCCGGA 1 cut(s) 1027
BseBI CCWGG 5 cut(s) 227, 951, 2051, 2352, 2591
BseDI CCNNGG 7 cut(s) 225, 336, 448, 673, 957, 2256, 2475
BseGI GGATG 6 cut(s) 281, 374, 1218, 1447, 1656, 2240
BseJI GATNNNNATC 2 cut(s) 534, 731
BseLI CCNNNNNNNGG 7 cut(s) 69, 232, 454, 625, 1330, 1890, 2414
BseMI GCAATG 2 cut(s) 1044, 2004
BseMII CTCAG 3 cut(s) 155, 2241, 2256
BseNI ACTGG 8 cut(s) 606, 629, 769, 895, 944, 1735, 2419, 2452
BseRI GAGGAG 5 cut(s) 43, 260, 1254, 2553, 2556
BseXI GCAGC 5 cut(s) 157, 499, 777, 1271, 2005
Bsh1236I CGCG 1 cut(s) 12
Bsh1285I CGRYCG 1 cut(s) 74
BshFI GGCC 4 cut(s) 1011, 1318, 1494, 1883
BsiEI CGRYCG 1 cut(s) 74
BsiHKAI GWGCWC 1 cut(s) 1582
BsiHKCI CYCGRG 1 cut(s) 1794
BsiSI CCGG 3 cut(s) 817, 1028, 1800
BslFI GGGAC 3 cut(s) 73, 2172, 2377
BslI CCNNNNNNNGG 7 cut(s) 69, 232, 454, 625, 1330, 1890, 2414
BsmAI GTCTC 8 cut(s) 9, 138, 177, 890, 1743, 2023, 2246, 2507
BsmBI CGTCTC 2 cut(s) 138, 2023
BsmFI GGGAC 3 cut(s) 73, 2172, 2377
BsmI GAATGC 2 cut(s) 503, 2361
BsnI GGCC 4 cut(s) 1011, 1318, 1494, 1883
Bso31I GGTCTC 2 cut(s) 890, 2246
BsoBI CYCGRG 1 cut(s) 1794
Bsp1286I GDGCHC 1 cut(s) 1582
Bsp13I TCCGGA 1 cut(s) 1027
Bsp1407I TGTACA 1 cut(s) 1983
Bsp143I GATC 9 cut(s) 235, 313, 535, 953, 1016, 1259, 1849, 2104, 2479
Bsp1720I GCTNAGC 1 cut(s) 141
Bsp19I CCATGG 3 cut(s) 448, 673, 2475
BspACI CCGC 6 cut(s) 10, 1042, 1186, 1566, 1933, 2303
BspANI GGCC 4 cut(s) 1011, 1318, 1494, 1883
BspCNI CTCAG 3 cut(s) 154, 2242, 2257
BspEI TCCGGA 1 cut(s) 1027
BspFNI CGCG 1 cut(s) 12
BspLI GGNNCC 7 cut(s) 158, 237, 325, 369, 955, 1714, 1951
BspMAI CTGCAG 1 cut(s) 389
BspPI GGATC 7 cut(s) 230, 243, 948, 961, 1267, 1844, 2487
BspTI CTTAAG 2 cut(s) 821, 1546
BspTNI GGTCTC 2 cut(s) 890, 2246
BsrDI GCAATG 2 cut(s) 1044, 2004
BsrFI RCCGGY 2 cut(s) 816, 1799
BsrGI TGTACA 1 cut(s) 1983
BsrI ACTGG 8 cut(s) 606, 629, 769, 895, 944, 1735, 2419, 2452
BssAI RCCGGY 2 cut(s) 816, 1799
BssECI CCNNGG 7 cut(s) 225, 336, 448, 673, 957, 2256, 2475
BssMI GATC 9 cut(s) 235, 313, 535, 953, 1016, 1259, 1849, 2104, 2479
BssSI CACGAG 1 cut(s) 736
BssT1I CCWWGG 5 cut(s) 336, 448, 673, 957, 2475
Bst2BI CACGAG 1 cut(s) 736
Bst2UI CCWGG 5 cut(s) 227, 951, 2051, 2352, 2591
Bst4CI ACNGT 2 cut(s) 72, 1563
Bst6I CTCTTC 1 cut(s) 180
BstAFI CTTAAG 2 cut(s) 821, 1546
BstAPI GCANNNNNTGC 1 cut(s) 1475
BstAUI TGTACA 1 cut(s) 1983
BstC8I GCNNGC 1 cut(s) 1578
BstDEI CTNAG 5 cut(s) 141, 642, 2250, 2265, 2511
BstDSI CCRYGG 4 cut(s) 448, 673, 2256, 2475
BstF5I GGATG 6 cut(s) 281, 374, 1218, 1447, 1656, 2240
BstFNI CGCG 1 cut(s) 12
BstHHI GCGC 2 cut(s) 14, 1734
BstKTI GATC 9 cut(s) 238, 316, 538, 956, 1019, 1262, 1852, 2107, 2482
BstMAI GTCTC 8 cut(s) 9, 138, 177, 890, 1743, 2023, 2246, 2507
BstMBI GATC 9 cut(s) 235, 313, 535, 953, 1016, 1259, 1849, 2104, 2479
BstMCI CGRYCG 1 cut(s) 74
BstMWI GCNNNNNNNGC 7 cut(s) 154, 509, 1063, 1426, 1475, 1915, 2152
BstNI CCWGG 5 cut(s) 227, 951, 2051, 2352, 2591
BstNSI RCATGY 2 cut(s) 855, 1922
BstSCI CCNGG 5 cut(s) 225, 949, 2049, 2350, 2589
BstSFI CTRYAG 2 cut(s) 385, 1249
BstUI CGCG 1 cut(s) 12
BstV1I GCAGC 5 cut(s) 157, 499, 777, 1271, 2005
BstV2I GAAGAC 4 cut(s) 212, 261, 1152, 2337
BstX2I RGATCY 3 cut(s) 235, 953, 1259
BstYI RGATCY 3 cut(s) 235, 953, 1259
BsuI GTATCC 1 cut(s) 2482
BsuRI GGCC 4 cut(s) 1011, 1318, 1494, 1883
BtgI CCRYGG 4 cut(s) 448, 673, 2256, 2475
BtrI CACGTC 1 cut(s) 1976
BtsCI GGATG 6 cut(s) 281, 374, 1218, 1447, 1656, 2240
BtsIMutI CAGTG 4 cut(s) 888, 1568, 1831, 2412
Cac8I GCNNGC 1 cut(s) 1578
CfoI GCGC 2 cut(s) 14, 1734
Cfr10I RCCGGY 2 cut(s) 816, 1799
Cfr13I GGNCC 4 cut(s) 323, 1240, 1493, 1882
CseI GACGC 1 cut(s) 1454
Csp6I GTAC 3 cut(s) 1298, 1984, 2445
CspCI CAANNNNNGTGG 2 cut(s) 2401, 2436
CviQI GTAC 3 cut(s) 1298, 1984, 2445
DdeI CTNAG 5 cut(s) 141, 642, 2250, 2265, 2511
DpnI GATC 9 cut(s) 237, 315, 537, 955, 1018, 1261, 1851, 2106, 2481
DpnII GATC 9 cut(s) 235, 313, 535, 953, 1016, 1259, 1849, 2104, 2479
DraI TTTAAA 1 cut(s) 865
DraIII CACNNNGTG 1 cut(s) 1616
DriI GACNNNNNGTC 1 cut(s) 169
EaeI YGGCCR 2 cut(s) 1009, 1316
Eam1104I CTCTTC 1 cut(s) 180
Eam1105I GACNNNNNGTC 1 cut(s) 169
EarI CTCTTC 1 cut(s) 180
EciI GGCGGA 1 cut(s) 1922
Eco130I CCWWGG 5 cut(s) 336, 448, 673, 957, 2475
Eco31I GGTCTC 2 cut(s) 890, 2246
Eco47I GGWCC 2 cut(s) 323, 1240
Eco88I CYCGRG 1 cut(s) 1794
EcoO109I RGGNCCY 2 cut(s) 323, 1240
EcoRI GAATTC 1 cut(s) 635
EcoRII CCWGG 5 cut(s) 225, 949, 2049, 2350, 2589
EcoT14I CCWWGG 5 cut(s) 336, 448, 673, 957, 2475
ErhI CCWWGG 5 cut(s) 336, 448, 673, 957, 2475
Esp3I CGTCTC 2 cut(s) 138, 2023
FalI AAGNNNNNCTT 4 cut(s) 12, 44, 2061, 2093
FaqI GGGAC 3 cut(s) 73, 2172, 2377
FauI CCCGC 3 cut(s) 1179, 1559, 2296
FbaI TGATCA 1 cut(s) 313
FblI GTMKAC 1 cut(s) 59
Fnu4HI GCNGC 5 cut(s) 146, 513, 766, 1285, 1994
FokI GGATG 6 cut(s) 288, 361, 1205, 1454, 1663, 2227
Fsp4HI GCNGC 5 cut(s) 146, 513, 766, 1285, 1994
FspBI CTAG 8 cut(s) 114, 200, 479, 857, 1070, 2280, 2424, 2570
GlaI GCGC 2 cut(s) 13, 1733
GluI GCNGC 5 cut(s) 146, 513, 766, 1285, 1994
HaeIII GGCC 4 cut(s) 1011, 1318, 1494, 1883
HapII CCGG 3 cut(s) 817, 1028, 1800
HgaI GACGC 1 cut(s) 1454
HhaI GCGC 2 cut(s) 14, 1734
Hin6I GCGC 2 cut(s) 12, 1732
HinP1I GCGC 2 cut(s) 12, 1732
HincII GTYRAC 3 cut(s) 60, 1747, 2285
HindII GTYRAC 3 cut(s) 60, 1747, 2285
HinfI GANTC 8 cut(s) 545, 1352, 1816, 1861, 2195, 2261, 2376, 2491
HpaII CCGG 3 cut(s) 817, 1028, 1800
HphI GGTGA 2 cut(s) 545, 1628
Hpy166II GTNNAC 5 cut(s) 60, 670, 1747, 1984, 2285
Hpy188I TCNGA 8 cut(s) 349, 405, 495, 778, 1437, 2113, 2251, 2266
Hpy8I GTNNAC 5 cut(s) 60, 670, 1747, 1984, 2285
Hpy99I CGWCG 3 cut(s) 61, 79, 2439
HpyCH4III ACNGT 2 cut(s) 72, 1563
HpyCH4IV ACGT 1 cut(s) 1975
HpyF10VI GCNNNNNNNGC 7 cut(s) 154, 509, 1063, 1426, 1475, 1915, 2152
HpyF3I CTNAG 5 cut(s) 141, 642, 2250, 2265, 2511
HpySE526I ACGT 1 cut(s) 1975
HspAI GCGC 2 cut(s) 12, 1732
Kpn2I TCCGGA 1 cut(s) 1027
Ksp22I TGATCA 1 cut(s) 313
Kzo9I GATC 9 cut(s) 235, 313, 535, 953, 1016, 1259, 1849, 2104, 2479
LmnI GCTCC 6 cut(s) 162, 970, 1030, 1398, 1417, 2566
Lsp1109I GCAGC 5 cut(s) 157, 499, 777, 1271, 2005
LweI GCATC 7 cut(s) 266, 383, 891, 1075, 1456, 1641, 2290
MaeI CTAG 8 cut(s) 114, 200, 479, 857, 1070, 2280, 2424, 2570
MaeII ACGT 1 cut(s) 1975
MaeIII GTNAC 4 cut(s) 1203, 1406, 1637, 2230
MalI GATC 9 cut(s) 237, 315, 537, 955, 1018, 1261, 1851, 2106, 2481
MboI GATC 9 cut(s) 235, 313, 535, 953, 1016, 1259, 1849, 2104, 2479
MboII GAAGA 7 cut(s) 167, 217, 266, 1157, 2229, 2342, 2626
MfeI CAATTG 2 cut(s) 498, 552
MflI RGATCY 3 cut(s) 235, 953, 1259
MhlI GDGCHC 1 cut(s) 1582
MlsI TGGCCA 2 cut(s) 1011, 1318
MluNI TGGCCA 2 cut(s) 1011, 1318
MlyI GAGTC 1 cut(s) 2385
MmeI TCCRAC 1 cut(s) 1420
Mox20I TGGCCA 2 cut(s) 1011, 1318
MroI TCCGGA 1 cut(s) 1027
MroXI GAANNNNTTC 1 cut(s) 1671
MscI TGGCCA 2 cut(s) 1011, 1318
MslI CAYNNNNRTG 3 cut(s) 274, 2325, 2522
Msp20I TGGCCA 2 cut(s) 1011, 1318
MspCI CTTAAG 2 cut(s) 821, 1546
MspI CCGG 3 cut(s) 817, 1028, 1800
MspR9I CCNGG 5 cut(s) 227, 951, 2051, 2352, 2591
MunI CAATTG 2 cut(s) 498, 552
Mva1269I GAATGC 2 cut(s) 503, 2361
MvaI CCWGG 5 cut(s) 227, 951, 2051, 2352, 2591
MvnI CGCG 1 cut(s) 12
MwoI GCNNNNNNNGC 7 cut(s) 154, 509, 1063, 1426, 1475, 1915, 2152
NcoI CCATGG 3 cut(s) 448, 673, 2475
NdeII GATC 9 cut(s) 235, 313, 535, 953, 1016, 1259, 1849, 2104, 2479
NlaIV GGNNCC 7 cut(s) 158, 237, 325, 369, 955, 1714, 1951
NmuCI GTSAC 1 cut(s) 2230
NspI RCATGY 2 cut(s) 855, 1922
PaeR7I CTCGAG 1 cut(s) 1794
PctI GAATGC 2 cut(s) 503, 2361
PdmI GAANNNNTTC 1 cut(s) 1671
PfeI GAWTC 7 cut(s) 545, 1352, 1816, 1861, 2195, 2261, 2491
PflFI GACNNNGTC 1 cut(s) 1151
PfoI TCCNGGA 2 cut(s) 2049, 2589
PkrI GCNGC 5 cut(s) 147, 514, 767, 1286, 1995
PleI GAGTC 1 cut(s) 2384
PpsI GAGTC 1 cut(s) 2384
PpuMI RGGWCCY 2 cut(s) 323, 1240
PsiI TTATAA 1 cut(s) 2641
Psp5II RGGWCCY 2 cut(s) 323, 1240
Psp6I CCWGG 5 cut(s) 225, 949, 2049, 2350, 2589
PspGI CCWGG 5 cut(s) 225, 949, 2049, 2350, 2589
PspN4I GGNNCC 7 cut(s) 158, 237, 325, 369, 955, 1714, 1951
PspPI GGNCC 4 cut(s) 323, 1240, 1493, 1882
PspPPI RGGWCCY 2 cut(s) 323, 1240
PspXI VCTCGAGB 1 cut(s) 1794
PstI CTGCAG 1 cut(s) 389
PsuI RGATCY 3 cut(s) 235, 953, 1259
PsyI GACNNNGTC 1 cut(s) 1151
RsaI GTAC 3 cut(s) 1299, 1985, 2446
RsaNI GTAC 3 cut(s) 1298, 1984, 2445
RseI CAYNNNNRTG 3 cut(s) 274, 2325, 2522
SalI GTCGAC 1 cut(s) 58
SatI GCNGC 5 cut(s) 146, 513, 766, 1285, 1994
Sau3AI GATC 9 cut(s) 235, 313, 535, 953, 1016, 1259, 1849, 2104, 2479
Sau96I GGNCC 4 cut(s) 323, 1240, 1493, 1882
SchI GAGTC 1 cut(s) 2385
ScrFI CCNGG 5 cut(s) 227, 951, 2051, 2352, 2591
SduI GDGCHC 1 cut(s) 1582
SfaNI GCATC 7 cut(s) 266, 383, 891, 1075, 1456, 1641, 2290
SfcI CTRYAG 2 cut(s) 385, 1249
Sfr274I CTCGAG 1 cut(s) 1794
SinI GGWCC 2 cut(s) 323, 1240
SlaI CTCGAG 1 cut(s) 1794
SmiMI CAYNNNNRTG 3 cut(s) 274, 2325, 2522
SmlI CTYRAG 5 cut(s) 151, 821, 1546, 1699, 1794
SmoI CTYRAG 5 cut(s) 151, 821, 1546, 1699, 1794
SsiI CCGC 6 cut(s) 10, 1042, 1186, 1566, 1933, 2303
SspI AATATT 1 cut(s) 1845
SspMI CTAG 8 cut(s) 114, 200, 479, 857, 1070, 2280, 2424, 2570
StyD4I CCNGG 5 cut(s) 225, 949, 2049, 2350, 2589
StyI CCWWGG 5 cut(s) 336, 448, 673, 957, 2475
TaaI ACNGT 2 cut(s) 72, 1563
TaiI ACGT 1 cut(s) 1978
TaqI TCGA 3 cut(s) 59, 804, 1795
TatI WGTACW 1 cut(s) 1983
TfiI GAWTC 7 cut(s) 545, 1352, 1816, 1861, 2195, 2261, 2491
TscAI CASTG 4 cut(s) 895, 1568, 1831, 2419
TseFI GTSAC 1 cut(s) 2230
TseI GCWGC 5 cut(s) 145, 512, 765, 1284, 1993
Tsp45I GTSAC 1 cut(s) 2230
TspDTI ATGAA 9 cut(s) 345, 843, 1008, 1344, 1974, 2163, 2229, 2343, 2506
TspGWI ACGGA 1 cut(s) 2273
TspRI CASTG 4 cut(s) 895, 1568, 1831, 2419
Tth111I GACNNNGTC 1 cut(s) 1151
Vha464I CTTAAG 2 cut(s) 821, 1546
VpaK11BI GGWCC 2 cut(s) 323, 1240
XapI RAATTY 6 cut(s) 635, 756, 860, 1670, 2085, 2537
XbaI TCTAGA 2 cut(s) 856, 2569
XceI RCATGY 2 cut(s) 855, 1922
XcmI CCANNNNNNNNNTGG 1 cut(s) 133
XhoI CTCGAG 1 cut(s) 1794
XmiI GTMKAC 1 cut(s) 59
XmnI GAANNNNTTC 1 cut(s) 1671
XspI CTAG 8 cut(s) 114, 200, 479, 857, 1070, 2280, 2424, 2570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.