Rh2AG323300

DNA excision repair protein ERCC-6-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0013205
Physical Location & Seq
Forward (+)
46484496 .. 46490408
5913 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG323300.1

Sequence Viewer

Length: 2631 bp
ATGTCTCTCCGCGCCTTCAAAGAAAGCCTTAAACCCTGCCAAACCCAAAACTCCTTGTCGACCCAAACACAGTCGTCGTCGTCGTCAATTCTGTCCCAAATCCCAGAACCCACAATCCCTAGAAAGCCCCCCAAATCGTCTCTGGCTCAGCAGCTCCTGAGGCTCCAAGAACCTCTGTCTCTTCAACCACCTCCACCTCCACCGCCACCGCCCCTAGAGAAGACCCAGGTGGAGGAGGACGAAGACGACCCAGAACCACAGGGATGCTCTGGGACTAGTAAAGTTAGTAGCTTTCAGTTTGATCACACAGGACCCTATGAACCCTTGGTCTTATCCCATTTTCCTCTCATTCAGGTTCCTGCATCCATTAACTGCAGATTGCTTCCACATCAGAGGGAGGGAGTAAAGTTTCTATACAATATATACAAGAACAACCATGGAGGGGTTCTTGGAGATGACATGGGACTAGGCAAAACCATTCAAACAATTGCATTCTTGGCTGCTGTGTTTGGGAAAGATGGTGATTGCATTGATTCCACAATTCCAAATAGAAGTGAAATAGCAGAACGAAACCCTGTTCTCATAGTTTGCCCCAGTTCAGTTATTCACAACTGGGAGAGTGAATTCTCTAAGTGGTCTAACTTTAGTGTTGCTGTTTACCACGGTGCAAATCGGGATTTGATTTATGATAAATTGGAAGCTCATGGAGTTGAGATACTTATCACGAGCTTTGACACATACAGAATTTGTGGCAGCCAGTTATCAGATGTTAATTGGGAGATTGTTGTTGTCGATGAGGCACACCGGCTTAAGAATGAAAAATCAAAGCTCTACATAGCATGTCTAGAATTTAAAACCTTAAAACGAATTGGTCTCACTGGAACTATGATGCAGAACAAAATCATGGAATTGTTTAACCTGTTTGACTGGGTTGCACCTGGATCCTTGGGAACACGGGAGCATTTTCGGGAGTTTTATGATGAACCTCTCAAACATGGCCAAAGATCAACTGCTCCGGAAAGATTTGTCCGCATTGCTGATGAGCGAAAACAGCATCTAGTAGCTGTTCTTAATAAATACATGTTGAGAAGGACAAAAGAGGAAACCATTGGTCATCTTATGATGGGGAAGGAAGACAATGTCATATTTTGTTCAATGAGCGAATTGCAAAAGCGGGTGTACAGGCGAATGTTACAGCTACCAGACATCCAATGCCTGATAAATAAGGACCTTCCCTGTAGTTGTGGATCTCCTCTTACCCAAGTTGAGTGCTGCAAAAGGATTGTACCAGATGGAAAAATCTGGCCATACCTTCACAGGGATAACCCTGATGGTTGTGATTCATGCCCCTTTTGTATTGTCCTTCCTTGCCTTGTCAAGCTCCAACAGATAAGTAACCACTTGGAGCTTATTAAGCCTAATCCGAAGGATGACCCAGAAAAGCAAAAGAAAGATGCAGAGTTTGCCTCTGCTGTGTTTGGCCCAGATATTGATTTGGTGGGAGGCAACACTCAAAATGAGAGTTTTATGGGCTTAAGTGATGTTAAACAGTGTGGGAAAATGCGAGCACTTGAAAAGTTTATGTTCTCATGGATTGCACAGGGTGATAAAGTCCTTCTGTTCAGTTACTCTGTCAGGATGCTGGACATATTGGAAAAATTTCTTATACGCAAAGGTTATTGCTTCTCAAGACTTGATGGTTCCACACCAACCAACTTGCGCCAGTCTCTTGTTGACAACTTTAATTCAAGTCCAAGCAAACAGGTGTTCCTTATATCAACTCGAGCTGGTGGGCTTGGACTGAATCTTGTCAGTGCAAATCGTGTTGTAATATTTGATCCAAGCTGGAATCCTGCCCAAGACTTACAGGCCCAAGACAGGTCGTTTCGGTTCGGGCAGAAACGGCATGTTGTGGTTTTCCGCCTTCTTTCTGCTGGTTCCCTTGATGAACTTGTTTATTCACGTCAGGTGTACAAACAGCAGCTATCAAACATTGCTGTTTCTGGAAAAATGGAGAGACGATATTTTGAAGGTGTCCAGGATTGCAAAGACTTTCAAGGGGAACTTTTTGGAATTTGCAATTTATTTCGTGATCTTTCAGATAAGCTCTTTACCAGCGAGATATTTGAGTTGCATGAAAAGCAGGGACAAAGAGAAGGGGATTGTCATGGTAGGGAACAAGAATCAACCAAAATTACTTGCGTAAAAGAAGTGGGTGTGACATCTTCATCCATGTCTGAGACCACGGATTCTGAGAAGGCTTTAGCTAGTCAACCTGTACTTAAAGATGTGGGTATTGTCTATGCACATCGTAATGAAGACATTGTGAATAATAGACGTGGATTGCAAGGGACGATGGAAAGAATCATTCCTTGTAATGATAGCCTAAAACAGCCCCCCACTGGTGTTGCTAGGAGAAAGCGACGAGAGGGTACTGGTGGAAAGGAGAATGTATCTGTATCCATGGATCGCAAAAGGATTCAATTTAGTCTCCTTGCTAAGTTCATGGGTATGGGAGAGATTGAATTTAGCAAGTGGGTAGTATCTGCTCCTCCTCTAGAGAGGGAAAAGGTTATCCAGGACTACAAGGAGAGAAACAAGAAGAAACTCAACAGCGTTGATTTGTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helicase_C SNF2_N

Protein Analysis

876

Amino Acids

99.13

Weight (kDa)

8.16

Isoelectric Point (pI)

45.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 125 - 296 2.2e-08 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 130 - 404 3.5e-65 SNF2-related domain
DEAD PF00270 149 - 299 5.9e-06 DEAD/DEAH box helicase
Helicase_C PF00271 520 - 632 2.4e-23 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013205)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03750
fragaria_vesca FvH4_6g26480 FvH4_6g26480 FvH4_6g26480 FvH4_6g26480
malus_domestica MD17G1236900.v1.1
prunus_persica Prupe.3G139100_v2.0.a1
pyrus_communis pycom17g24160
rosa_chinensis RchiOBHm_Chr2g0127761
rosa_laevigata RLG00000018935
rosa_multiflora Rmu_sc0001829.1_g000008 Rmu_sc0004240.1_g000009
rosa_roxburghii Rroxscaffold_2G00116780
rosa_rugosa Rorug02G0271200
rosa_samantha Rh2AG323300 Rh2BG332500 Rh2CG311100
rosa_wichuraiana Rw2G026240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2629
AccI GTMKAC 1 cut(s) 59
AccII CGCG 1 cut(s) 12
AccIII TCCGGA 1 cut(s) 1015
AciI CCGC 6 cut(s) 10, 203, 209, 1030, 1174, 1921
AclWI GGATC 5 cut(s) 936, 949, 1255, 1832, 2475
AcoI YGGCCR 2 cut(s) 997, 1304
AcsI RAATTY 6 cut(s) 623, 744, 848, 1658, 2073, 2525
AdeI CACNNNGTG 1 cut(s) 1604
AfaI GTAC 5 cut(s) 1181, 1287, 1973, 2280, 2434
AfiI CCNNNNNNNGG 5 cut(s) 232, 442, 1318, 1878, 2402
AflII CTTAAG 2 cut(s) 809, 1534
AflIII ACRYGT 1 cut(s) 1080
AhlI ACTAGT 1 cut(s) 275
AjiI CACGTC 2 cut(s) 1964, 2339
AjnI CCWGG 4 cut(s) 225, 937, 2037, 2577
AloI GAACNNNNNNTCC 2 cut(s) 99, 131
Alw21I GWGCWC 1 cut(s) 1570
Alw26I GTCTC 8 cut(s) 9, 144, 183, 878, 1731, 2011, 2234, 2495
AlwI GGATC 5 cut(s) 936, 949, 1255, 1832, 2475
AlwNI CAGNNNCTG 1 cut(s) 157
Ama87I CYCGRG 1 cut(s) 1782
Aor13HI TCCGGA 1 cut(s) 1015
AoxI GGCC 4 cut(s) 997, 1304, 1480, 1869
ApeKI GCWGC 5 cut(s) 151, 500, 753, 1272, 1981
ApoI RAATTY 6 cut(s) 623, 744, 848, 1658, 2073, 2525
Asp700I GAANNNNTTC 1 cut(s) 1659
AspLEI GCGC 2 cut(s) 14, 1722
AspS9I GGNCC 4 cut(s) 311, 1228, 1481, 1870
AsuHPI GGTGA 2 cut(s) 533, 1616
AvaI CYCGRG 1 cut(s) 1782
AvaII GGWCC 2 cut(s) 311, 1228
AxyI CCTNAGG 1 cut(s) 158
BalI TGGCCA 2 cut(s) 999, 1306
BamHI GGATCC 1 cut(s) 941
BauI CACGAG 1 cut(s) 724
BbsI GAAGAC 4 cut(s) 227, 249, 1140, 2325
Bbv12I GWGCWC 1 cut(s) 1570
BbvI GCAGC 5 cut(s) 163, 487, 765, 1259, 1993
BccI CCATC 6 cut(s) 512, 1117, 1286, 1325, 1691, 2350
BceAI ACGGC 1 cut(s) 1919
BciT130I CCWGG 4 cut(s) 227, 939, 2039, 2579
BciVI GTATCC 1 cut(s) 2470
BclI TGATCA 1 cut(s) 301
BcoDI GTCTC 8 cut(s) 9, 144, 183, 878, 1731, 2011, 2234, 2495
BcuI ACTAGT 1 cut(s) 275
BfaI CTAG 9 cut(s) 120, 215, 276, 467, 845, 1058, 2268, 2412, 2558
BfmI CTRYAG 2 cut(s) 373, 1237
BfrI CTTAAG 2 cut(s) 809, 1534
BfuI GTATCC 1 cut(s) 2470
BisI GCNGC 5 cut(s) 152, 501, 754, 1273, 1982
BlpI GCTNAGC 1 cut(s) 147
BlsI GCNGC 5 cut(s) 153, 502, 755, 1274, 1983
Bme1390I CCNGG 4 cut(s) 227, 939, 2039, 2579
Bme18I GGWCC 2 cut(s) 311, 1228
BmeT110I CYCGRG 1 cut(s) 1782
BmgBI CACGTC 2 cut(s) 1964, 2339
BmgT120I GGNCC 4 cut(s) 311, 1228, 1481, 1870
BmiI GGNNCC 6 cut(s) 164, 313, 357, 943, 1702, 1939
BmrFI CCNGG 4 cut(s) 227, 939, 2039, 2579
BmrI ACTGGG 3 cut(s) 588, 622, 937
BmsI GCATC 6 cut(s) 254, 371, 879, 1063, 1444, 1629
BmuI ACTGGG 3 cut(s) 588, 622, 937
BpiI GAAGAC 4 cut(s) 227, 249, 1140, 2325
BplI GAGNNNNNCTC 2 cut(s) 1451, 1483
Bpu1102I GCTNAGC 1 cut(s) 147
BpuEI CTTGAG 1 cut(s) 1672
BsaBI GATNNNNATC 1 cut(s) 719
BsaI GGTCTC 2 cut(s) 878, 2234
BsaJI CCNNGG 7 cut(s) 225, 324, 436, 661, 945, 2244, 2463
BsaWI WCCGGW 1 cut(s) 1015
Bsc4I CCNNNNNNNGG 5 cut(s) 232, 442, 1318, 1878, 2402
Bse118I RCCGGY 1 cut(s) 804
Bse1I ACTGG 8 cut(s) 594, 617, 757, 883, 932, 1723, 2407, 2440
Bse21I CCTNAGG 1 cut(s) 158
Bse3DI GCAATG 2 cut(s) 1032, 1992
Bse8I GATNNNNATC 1 cut(s) 719
BseAI TCCGGA 1 cut(s) 1015
BseBI CCWGG 4 cut(s) 227, 939, 2039, 2579
BseDI CCNNGG 7 cut(s) 225, 324, 436, 661, 945, 2244, 2463
BseGI GGATG 6 cut(s) 269, 362, 1206, 1435, 1644, 2228
BseJI GATNNNNATC 1 cut(s) 719
BseLI CCNNNNNNNGG 5 cut(s) 232, 442, 1318, 1878, 2402
BseMI GCAATG 2 cut(s) 1032, 1992
BseMII CTCAG 4 cut(s) 149, 161, 2229, 2244
BseNI ACTGG 8 cut(s) 594, 617, 757, 883, 932, 1723, 2407, 2440
BseRI GAGGAG 4 cut(s) 248, 1242, 2541, 2544
BseXI GCAGC 5 cut(s) 163, 487, 765, 1259, 1993
Bsh1236I CGCG 1 cut(s) 12
BshFI GGCC 4 cut(s) 999, 1306, 1482, 1871
BsiHKAI GWGCWC 1 cut(s) 1570
BsiHKCI CYCGRG 1 cut(s) 1782
BsiSI CCGG 2 cut(s) 805, 1016
BslFI GGGAC 5 cut(s) 79, 286, 477, 2160, 2365
BslI CCNNNNNNNGG 5 cut(s) 232, 442, 1318, 1878, 2402
BsmAI GTCTC 8 cut(s) 9, 144, 183, 878, 1731, 2011, 2234, 2495
BsmBI CGTCTC 2 cut(s) 144, 2011
BsmFI GGGAC 5 cut(s) 79, 286, 477, 2160, 2365
BsmI GAATGC 1 cut(s) 491
BsnI GGCC 4 cut(s) 999, 1306, 1482, 1871
Bso31I GGTCTC 2 cut(s) 878, 2234
BsoBI CYCGRG 1 cut(s) 1782
Bsp1286I GDGCHC 1 cut(s) 1570
Bsp13I TCCGGA 1 cut(s) 1015
Bsp1407I TGTACA 2 cut(s) 1179, 1971
Bsp143I GATC 7 cut(s) 301, 941, 1004, 1247, 1837, 2092, 2467
Bsp1720I GCTNAGC 1 cut(s) 147
Bsp19I CCATGG 2 cut(s) 436, 2463
BspACI CCGC 6 cut(s) 10, 203, 209, 1030, 1174, 1921
BspANI GGCC 4 cut(s) 999, 1306, 1482, 1871
BspCNI CTCAG 4 cut(s) 150, 160, 2230, 2245
BspEI TCCGGA 1 cut(s) 1015
BspFNI CGCG 1 cut(s) 12
BspLI GGNNCC 6 cut(s) 164, 313, 357, 943, 1702, 1939
BspMAI CTGCAG 1 cut(s) 377
BspPI GGATC 5 cut(s) 936, 949, 1255, 1832, 2475
BspTI CTTAAG 2 cut(s) 809, 1534
BspTNI GGTCTC 2 cut(s) 878, 2234
BsrDI GCAATG 2 cut(s) 1032, 1992
BsrFI RCCGGY 1 cut(s) 804
BsrGI TGTACA 2 cut(s) 1179, 1971
BsrI ACTGG 8 cut(s) 594, 617, 757, 883, 932, 1723, 2407, 2440
BssAI RCCGGY 1 cut(s) 804
BssECI CCNNGG 7 cut(s) 225, 324, 436, 661, 945, 2244, 2463
BssMI GATC 7 cut(s) 301, 941, 1004, 1247, 1837, 2092, 2467
BssSI CACGAG 1 cut(s) 724
BssT1I CCWWGG 4 cut(s) 324, 436, 945, 2463
Bst2BI CACGAG 1 cut(s) 724
Bst2UI CCWGG 4 cut(s) 227, 939, 2039, 2579
Bst4CI ACNGT 3 cut(s) 72, 665, 1551
Bst6I CTCTTC 1 cut(s) 186
BstAFI CTTAAG 2 cut(s) 809, 1534
BstAPI GCANNNNNTGC 1 cut(s) 1463
BstAUI TGTACA 2 cut(s) 1179, 1971
BstC8I GCNNGC 1 cut(s) 1566
BstDEI CTNAG 6 cut(s) 147, 158, 630, 2238, 2253, 2499
BstDSI CCRYGG 4 cut(s) 436, 661, 2244, 2463
BstF5I GGATG 6 cut(s) 269, 362, 1206, 1435, 1644, 2228
BstFNI CGCG 1 cut(s) 12
BstHHI GCGC 2 cut(s) 14, 1722
BstKTI GATC 7 cut(s) 304, 944, 1007, 1250, 1840, 2095, 2470
BstMAI GTCTC 8 cut(s) 9, 144, 183, 878, 1731, 2011, 2234, 2495
BstMBI GATC 7 cut(s) 301, 941, 1004, 1247, 1837, 2092, 2467
BstMWI GCNNNNNNNGC 7 cut(s) 160, 497, 1051, 1414, 1463, 1903, 2140
BstNI CCWGG 4 cut(s) 227, 939, 2039, 2579
BstNSI RCATGY 3 cut(s) 843, 1084, 1910
BstSCI CCNGG 4 cut(s) 225, 937, 2037, 2577
BstSFI CTRYAG 2 cut(s) 373, 1237
BstUI CGCG 1 cut(s) 12
BstV1I GCAGC 5 cut(s) 163, 487, 765, 1259, 1993
BstV2I GAAGAC 4 cut(s) 227, 249, 1140, 2325
BstX2I RGATCY 2 cut(s) 941, 1247
BstYI RGATCY 2 cut(s) 941, 1247
Bsu36I CCTNAGG 1 cut(s) 158
BsuI GTATCC 1 cut(s) 2470
BsuRI GGCC 4 cut(s) 999, 1306, 1482, 1871
BtgI CCRYGG 4 cut(s) 436, 661, 2244, 2463
BtrI CACGTC 2 cut(s) 1964, 2339
BtsCI GGATG 6 cut(s) 269, 362, 1206, 1435, 1644, 2228
BtsIMutI CAGTG 4 cut(s) 876, 1556, 1819, 2400
Cac8I GCNNGC 1 cut(s) 1566
CaiI CAGNNNCTG 1 cut(s) 157
CfoI GCGC 2 cut(s) 14, 1722
Cfr10I RCCGGY 1 cut(s) 804
Cfr13I GGNCC 4 cut(s) 311, 1228, 1481, 1870
Csp6I GTAC 5 cut(s) 1180, 1286, 1972, 2279, 2433
CspCI CAANNNNNGTGG 2 cut(s) 2389, 2424
CviQI GTAC 5 cut(s) 1180, 1286, 1972, 2279, 2433
DdeI CTNAG 6 cut(s) 147, 158, 630, 2238, 2253, 2499
DpnI GATC 7 cut(s) 303, 943, 1006, 1249, 1839, 2094, 2469
DpnII GATC 7 cut(s) 301, 941, 1004, 1247, 1837, 2092, 2467
DraI TTTAAA 1 cut(s) 853
DraIII CACNNNGTG 1 cut(s) 1604
EaeI YGGCCR 2 cut(s) 997, 1304
Eam1104I CTCTTC 1 cut(s) 186
EarI CTCTTC 1 cut(s) 186
EciI GGCGGA 1 cut(s) 1910
Eco130I CCWWGG 4 cut(s) 324, 436, 945, 2463
Eco31I GGTCTC 2 cut(s) 878, 2234
Eco47I GGWCC 2 cut(s) 311, 1228
Eco81I CCTNAGG 1 cut(s) 158
Eco88I CYCGRG 1 cut(s) 1782
EcoO109I RGGNCCY 2 cut(s) 311, 1228
EcoRI GAATTC 1 cut(s) 623
EcoRII CCWGG 4 cut(s) 225, 937, 2037, 2577
EcoT14I CCWWGG 4 cut(s) 324, 436, 945, 2463
ErhI CCWWGG 4 cut(s) 324, 436, 945, 2463
Esp3I CGTCTC 2 cut(s) 144, 2011
FalI AAGNNNNNCTT 4 cut(s) 12, 44, 2049, 2081
FaqI GGGAC 5 cut(s) 79, 286, 477, 2160, 2365
FauI CCCGC 1 cut(s) 1167
FbaI TGATCA 1 cut(s) 301
FblI GTMKAC 1 cut(s) 59
Fnu4HI GCNGC 5 cut(s) 152, 501, 754, 1273, 1982
FokI GGATG 6 cut(s) 276, 349, 1193, 1442, 1651, 2215
Fsp4HI GCNGC 5 cut(s) 152, 501, 754, 1273, 1982
FspBI CTAG 9 cut(s) 120, 215, 276, 467, 845, 1058, 2268, 2412, 2558
GlaI GCGC 2 cut(s) 13, 1721
GluI GCNGC 5 cut(s) 152, 501, 754, 1273, 1982
HaeIII GGCC 4 cut(s) 999, 1306, 1482, 1871
HapII CCGG 2 cut(s) 805, 1016
HhaI GCGC 2 cut(s) 14, 1722
Hin6I GCGC 2 cut(s) 12, 1720
HinP1I GCGC 2 cut(s) 12, 1720
HincII GTYRAC 3 cut(s) 60, 1735, 2273
HindII GTYRAC 3 cut(s) 60, 1735, 2273
HinfI GANTC 8 cut(s) 533, 1340, 1804, 1849, 2183, 2249, 2364, 2479
HpaII CCGG 2 cut(s) 805, 1016
HphI GGTGA 2 cut(s) 533, 1616
Hpy166II GTNNAC 6 cut(s) 60, 658, 1180, 1735, 1972, 2273
Hpy188I TCNGA 6 cut(s) 393, 766, 1425, 2101, 2239, 2254
Hpy8I GTNNAC 6 cut(s) 60, 658, 1180, 1735, 1972, 2273
Hpy99I CGWCG 4 cut(s) 79, 82, 85, 2427
HpyCH4III ACNGT 3 cut(s) 72, 665, 1551
HpyCH4IV ACGT 2 cut(s) 1963, 2338
HpyF10VI GCNNNNNNNGC 7 cut(s) 160, 497, 1051, 1414, 1463, 1903, 2140
HpyF3I CTNAG 6 cut(s) 147, 158, 630, 2238, 2253, 2499
HpySE526I ACGT 2 cut(s) 1963, 2338
HspAI GCGC 2 cut(s) 12, 1720
Kpn2I TCCGGA 1 cut(s) 1015
Ksp22I TGATCA 1 cut(s) 301
Kzo9I GATC 7 cut(s) 301, 941, 1004, 1247, 1837, 2092, 2467
LmnI GCTCC 7 cut(s) 159, 168, 958, 1018, 1386, 1405, 2554
Lsp1109I GCAGC 5 cut(s) 163, 487, 765, 1259, 1993
LweI GCATC 6 cut(s) 254, 371, 879, 1063, 1444, 1629
MaeI CTAG 9 cut(s) 120, 215, 276, 467, 845, 1058, 2268, 2412, 2558
MaeII ACGT 2 cut(s) 1963, 2338
MaeIII GTNAC 4 cut(s) 1191, 1394, 1625, 2218
MalI GATC 7 cut(s) 303, 943, 1006, 1249, 1839, 2094, 2469
MboI GATC 7 cut(s) 301, 941, 1004, 1247, 1837, 2092, 2467
MboII GAAGA 7 cut(s) 173, 232, 254, 1145, 2217, 2330, 2614
MfeI CAATTG 1 cut(s) 486
MflI RGATCY 2 cut(s) 941, 1247
MhlI GDGCHC 1 cut(s) 1570
MlsI TGGCCA 2 cut(s) 999, 1306
MluNI TGGCCA 2 cut(s) 999, 1306
MmeI TCCRAC 1 cut(s) 1408
Mox20I TGGCCA 2 cut(s) 999, 1306
MroI TCCGGA 1 cut(s) 1015
MroXI GAANNNNTTC 1 cut(s) 1659
MscI TGGCCA 2 cut(s) 999, 1306
MslI CAYNNNNRTG 3 cut(s) 262, 2313, 2510
Msp20I TGGCCA 2 cut(s) 999, 1306
MspCI CTTAAG 2 cut(s) 809, 1534
MspI CCGG 2 cut(s) 805, 1016
MspR9I CCNGG 4 cut(s) 227, 939, 2039, 2579
MunI CAATTG 1 cut(s) 486
Mva1269I GAATGC 1 cut(s) 491
MvaI CCWGG 4 cut(s) 227, 939, 2039, 2579
MvnI CGCG 1 cut(s) 12
MwoI GCNNNNNNNGC 7 cut(s) 160, 497, 1051, 1414, 1463, 1903, 2140
NcoI CCATGG 2 cut(s) 436, 2463
NdeII GATC 7 cut(s) 301, 941, 1004, 1247, 1837, 2092, 2467
NlaIV GGNNCC 6 cut(s) 164, 313, 357, 943, 1702, 1939
NmuCI GTSAC 1 cut(s) 2218
NspI RCATGY 3 cut(s) 843, 1084, 1910
PaeR7I CTCGAG 1 cut(s) 1782
PciI ACATGT 1 cut(s) 1080
PcsI WCGNNNNNNNCGW 1 cut(s) 80
PctI GAATGC 1 cut(s) 491
PdmI GAANNNNTTC 1 cut(s) 1659
PfeI GAWTC 8 cut(s) 533, 1340, 1804, 1849, 2183, 2249, 2364, 2479
PflFI GACNNNGTC 1 cut(s) 1139
PfoI TCCNGGA 2 cut(s) 2037, 2577
PkrI GCNGC 5 cut(s) 153, 502, 755, 1274, 1983
PpuMI RGGWCCY 2 cut(s) 311, 1228
PscI ACATGT 1 cut(s) 1080
PsiI TTATAA 1 cut(s) 2629
Psp5II RGGWCCY 2 cut(s) 311, 1228
Psp6I CCWGG 4 cut(s) 225, 937, 2037, 2577
PspGI CCWGG 4 cut(s) 225, 937, 2037, 2577
PspN4I GGNNCC 6 cut(s) 164, 313, 357, 943, 1702, 1939
PspPI GGNCC 4 cut(s) 311, 1228, 1481, 1870
PspPPI RGGWCCY 2 cut(s) 311, 1228
PspXI VCTCGAGB 1 cut(s) 1782
PstI CTGCAG 1 cut(s) 377
PstNI CAGNNNCTG 1 cut(s) 157
PsuI RGATCY 2 cut(s) 941, 1247
PsyI GACNNNGTC 1 cut(s) 1139
RsaI GTAC 5 cut(s) 1181, 1287, 1973, 2280, 2434
RsaNI GTAC 5 cut(s) 1180, 1286, 1972, 2279, 2433
RseI CAYNNNNRTG 3 cut(s) 262, 2313, 2510
SalI GTCGAC 1 cut(s) 58
SatI GCNGC 5 cut(s) 152, 501, 754, 1273, 1982
Sau3AI GATC 7 cut(s) 301, 941, 1004, 1247, 1837, 2092, 2467
Sau96I GGNCC 4 cut(s) 311, 1228, 1481, 1870
ScrFI CCNGG 4 cut(s) 227, 939, 2039, 2579
SduI GDGCHC 1 cut(s) 1570
SfaNI GCATC 6 cut(s) 254, 371, 879, 1063, 1444, 1629
SfcI CTRYAG 2 cut(s) 373, 1237
Sfr274I CTCGAG 1 cut(s) 1782
SinI GGWCC 2 cut(s) 311, 1228
SlaI CTCGAG 1 cut(s) 1782
SmiMI CAYNNNNRTG 3 cut(s) 262, 2313, 2510
SmlI CTYRAG 4 cut(s) 809, 1534, 1687, 1782
SmoI CTYRAG 4 cut(s) 809, 1534, 1687, 1782
SpeI ACTAGT 1 cut(s) 275
SsiI CCGC 6 cut(s) 10, 203, 209, 1030, 1174, 1921
SspI AATATT 1 cut(s) 1833
SspMI CTAG 9 cut(s) 120, 215, 276, 467, 845, 1058, 2268, 2412, 2558
StyD4I CCNGG 4 cut(s) 225, 937, 2037, 2577
StyI CCWWGG 4 cut(s) 324, 436, 945, 2463
TaaI ACNGT 3 cut(s) 72, 665, 1551
TaiI ACGT 2 cut(s) 1966, 2341
TaqI TCGA 3 cut(s) 59, 792, 1783
TatI WGTACW 3 cut(s) 1179, 1971, 2278
TfiI GAWTC 8 cut(s) 533, 1340, 1804, 1849, 2183, 2249, 2364, 2479
TscAI CASTG 4 cut(s) 883, 1556, 1819, 2407
TseFI GTSAC 1 cut(s) 2218
TseI GCWGC 5 cut(s) 151, 500, 753, 1272, 1981
Tsp45I GTSAC 1 cut(s) 2218
TspDTI ATGAA 9 cut(s) 333, 831, 996, 1332, 1962, 2151, 2217, 2331, 2494
TspGWI ACGGA 1 cut(s) 2261
TspRI CASTG 4 cut(s) 883, 1556, 1819, 2407
Tth111I GACNNNGTC 1 cut(s) 1139
Vha464I CTTAAG 2 cut(s) 809, 1534
VpaK11BI GGWCC 2 cut(s) 311, 1228
XapI RAATTY 6 cut(s) 623, 744, 848, 1658, 2073, 2525
XbaI TCTAGA 2 cut(s) 844, 2557
XceI RCATGY 3 cut(s) 843, 1084, 1910
XcmI CCANNNNNNNNNTGG 1 cut(s) 139
XhoI CTCGAG 1 cut(s) 1782
XmiI GTMKAC 1 cut(s) 59
XmnI GAANNNNTTC 1 cut(s) 1659
XspI CTAG 9 cut(s) 120, 215, 276, 467, 845, 1058, 2268, 2412, 2558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.