Rw2G026240

DNA excision repair protein ERCC-6-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0013205
Physical Location & Seq
Forward (+)
40460084 .. 40466223
6140 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G026240.1

Sequence Viewer

Length: 2634 bp
ATGTCTCTCCGCGCCTTCAAAGAGAGCCTTAAACCCTGCCAAACCCAAAACTCCTCGTCGACCCAAACACAGTCGTCGTCGTCGTCGTCAATTCTGTCCCAAATCCCAGAACCCACAATCCCTAGAAAGCCCCCCAAATCGTCTCTGGCTCAGCAGCTCCTGAGGCTCCAAGACCCTCTGTCTCTTCAACCACCTCCACCTCCACCGCCACCGCCCCTAGAGAAGACCCAGGTGGAGGAGGACGAAGACGACCCAGAACCACAGGGATGCTCTGGGACTAGTAAAGTTAGTAGCTTTCAGTTTGATCACACAGGACCCTATGAACCCTTGGTCTTATCCCATTTTCCTCTCATTCAGGTTCCTGCATCCATTAACTGCAGATTGCTTCCACATCAGAGGGAGGGAGTAAAGTTTCTATACAATATATACAAGAACAACCATGGAGGGGTTCTTGGAGATGACATGGGACTAGGCAAAACCATTCAAACAATTGCATTCTTGGCTGCTGTGTTTGGGAAAGATGGTGATTGCATTGATTCTACAATTCCGATGAGAAGTGAAATAGCAGAACGAAACCCTGTTCTCATAGTTTGCCCCAGTTCAGTTATCCACAACTGGGAGAGTGAATTCTCTAAGTGGTCTAACTTTAGTGTTGCTGTTTACCATGGTGCAAATCGGGATTTGATTTATGATAAATTGGAAGCTCATGGAGTTGAGATACTTATCACGAGCTTTGACACATACAGAATTTGTGGCAGCCAGTTATCAGATGTTAATTGGGAGATTGTTGTTGTCGATGAGGCACACCGGCTTAAGAATGAAAAATCAAAGCTCTACATAGCATGTCTAGAATTTAAAACCTTAAAACGAATTGGTCTCACTGGAACTATGATGCAGAACAAAATCATGGAATTGTTTAACCTGTTTGACTGGGTTGCACCTGGATCCTTGGGAACACGGGAGCATTTTCGGGAGTTTTATGATGAACCTCTCAAACATGGCCAAAGATCAACTGCTCCAGAAAGATTTGTCCGCATTGCTGATGAGCGAAAACAGCATCTAGTAGCTGTTCTTAATAAATACATGTTGAGAAGGACAAAAGAGGAAACCATTGGTCATCTTATGATGGGAAAGGAAGACAATGTCATATTTTGTTCAATGAGCGAATTGCAAAAGCGGGTGTATAGGCGAATGTTACAGCTACCAGACATCCAATGCCTGATAAATAAGGACCTTCCCTGTAGTTGTGGATCTCCTCTTACCCAAGTTGAGTGCTGCAAAAGGATTGTACCAGATGGAAAAATCTGGCCATACCTTCACAGGGATAACCCTGATGGTTGTGATTCATGCCCCTTTTGTATTGTCCTTCCTTGCCTTGTCAAGCTCCAACAGATAAGTAACCACTTGGAGCTTATTAAGCCTAATCCGAAGGATGACCCAGAAAAGCAAAAGAAAGATGCAGAGTTTGCCTCTGCTGTGTTTGGCCCAGATATTGATTTGGTGGGAGGCAACACTCAAAATGAGAGTTTTATGGGCTTAAGTGATGTTAAACAGTGTGGGAAAATGCGAGCACTTGAAAAGTTTATGTTCTCATGGATTGCACAGGGTGATAAAGTCCTTCTGTTCAGTTACTCTGTCAGGATGCTGGACATATTGGAAAAATTTCTTATACGCAAAGGTTATTGCTTCTCAAGACTTGATGGTTCCACACCAACCAACTTGCGCCAGTCTCTTGTTGACAACTTTAATTCAAGTCCAAGCAAACAGGTGTTCCTTATATCAACTCGAGCCGGTGGGCTTGGACTGAATCTTGTCAGTGCAAATCGTGTTGTAATATTTGATCCAAGCTGGAATCCTGCCCAAGACTTACAGGCCCAAGACAGGTCATTTCGGTTTGGGCAGAAACGGCATGTTGTGGTTTTCCGCCTTCTTTCTGCTGGTTCCCTTGATGAACTTGTTTATTCACGTCAGGTGTACAAACAGCAGCTATCAAACATTGCTGTTTCTGGAAAAATGGAGAGACGATATTTTGAAGGTGTCCAGGATTGCAAAGACTTTCAAGGGGAACTTTTTGGAATTTGCAATTTATTTCGTGATCTTTCAGATAAGCTCTTTACCAGCGAGATATTTGAGTTGCATGAAAAGCAGGGACAAAGAGAAGGGGATTGTCATGGTAGGGAACAAGAATCAACCAAAATTACTTGCGTAAAAGAAGTGGGTGTGACATCTTCATCCATGTCTGAGACCACGGATTCTGAGAAGGCTTTAGCTAGTCAACCTGTGCTTAAAGATGTGGGTATTGTCTATGCACATCGTAATGAAGACATTGTGAATAATAGACCTGGATTGCAAGGGACGATGGAAAGAGTCATTCCTTGTAATGATAGCCTAAAACAGCCCCCCACTGGTGTTGCTAGGAGAAAGCGACGAGAGGGTACTGGTGGAAAGGAGAATGTATCTGTATCCATGGATCGCAAAAGAATTCAATTTAGTCTCCTTGCTAAGTTCATGGGTATGGGAGAGATTGAATTTAGCAAGTGGGTAGTATCTGCTCCTCCTCTAGAGAGGGAAAAGGTTATCCAGGACTACAAGGAGAGGAACAAGAAGAAACTCAACAGCGTTGATTTGTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helicase_C SNF2_N

Protein Analysis

877

Amino Acids

99.12

Weight (kDa)

8.04

Isoelectric Point (pI)

45.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 126 - 297 2.2e-08 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 131 - 405 4e-65 SNF2-related domain
DEAD PF00270 151 - 300 5.9e-06 DEAD/DEAH box helicase
Helicase_C PF00271 521 - 633 2.4e-23 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013205)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03750
fragaria_vesca FvH4_6g26480 FvH4_6g26480 FvH4_6g26480 FvH4_6g26480
malus_domestica MD17G1236900.v1.1
prunus_persica Prupe.3G139100_v2.0.a1
pyrus_communis pycom17g24160
rosa_chinensis RchiOBHm_Chr2g0127761
rosa_laevigata RLG00000018935
rosa_multiflora Rmu_sc0001829.1_g000008 Rmu_sc0004240.1_g000009
rosa_roxburghii Rroxscaffold_2G00116780
rosa_rugosa Rorug02G0271200
rosa_samantha Rh2AG323300 Rh2BG332500 Rh2CG311100
rosa_wichuraiana Rw2G026240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2632
AccI GTMKAC 1 cut(s) 59
AccII CGCG 1 cut(s) 12
AciI CCGC 6 cut(s) 10, 206, 212, 1033, 1177, 1924
AclWI GGATC 5 cut(s) 939, 952, 1258, 1835, 2478
AcoI YGGCCR 2 cut(s) 1000, 1307
AcsI RAATTY 7 cut(s) 626, 747, 851, 1661, 2076, 2481, 2528
AdeI CACNNNGTG 1 cut(s) 1607
AfaI GTAC 3 cut(s) 1290, 1976, 2437
AfiI CCNNNNNNNGG 6 cut(s) 235, 445, 616, 1321, 1881, 2405
AflII CTTAAG 2 cut(s) 812, 1537
AflIII ACRYGT 1 cut(s) 1083
AhdI GACNNNNNGTC 1 cut(s) 178
AhlI ACTAGT 1 cut(s) 278
AjiI CACGTC 1 cut(s) 1967
AjnI CCWGG 5 cut(s) 228, 940, 2040, 2341, 2580
AloI GAACNNNNNNTCC 2 cut(s) 102, 134
Alw21I GWGCWC 1 cut(s) 1573
Alw26I GTCTC 8 cut(s) 9, 147, 186, 881, 1734, 2014, 2237, 2498
AlwI GGATC 5 cut(s) 939, 952, 1258, 1835, 2478
AlwNI CAGNNNCTG 1 cut(s) 160
Ama87I CYCGRG 1 cut(s) 1785
AoxI GGCC 4 cut(s) 1000, 1307, 1483, 1872
ApeKI GCWGC 5 cut(s) 154, 503, 756, 1275, 1984
ApoI RAATTY 7 cut(s) 626, 747, 851, 1661, 2076, 2481, 2528
Asp700I GAANNNNTTC 1 cut(s) 1662
AspLEI GCGC 2 cut(s) 14, 1725
AspS9I GGNCC 4 cut(s) 314, 1231, 1484, 1873
AsuHPI GGTGA 2 cut(s) 536, 1619
AvaI CYCGRG 1 cut(s) 1785
AvaII GGWCC 2 cut(s) 314, 1231
AxyI CCTNAGG 1 cut(s) 161
BalI TGGCCA 2 cut(s) 1002, 1309
BamHI GGATCC 1 cut(s) 944
BauI CACGAG 1 cut(s) 727
BbsI GAAGAC 4 cut(s) 230, 252, 1143, 2328
Bbv12I GWGCWC 1 cut(s) 1573
BbvI GCAGC 5 cut(s) 166, 490, 768, 1262, 1996
BccI CCATC 6 cut(s) 515, 1120, 1289, 1328, 1694, 2353
BceAI ACGGC 1 cut(s) 1922
BciT130I CCWGG 5 cut(s) 230, 942, 2042, 2343, 2582
BciVI GTATCC 1 cut(s) 2473
BclI TGATCA 1 cut(s) 304
BcoDI GTCTC 8 cut(s) 9, 147, 186, 881, 1734, 2014, 2237, 2498
BcuI ACTAGT 1 cut(s) 278
BfaI CTAG 9 cut(s) 123, 218, 279, 470, 848, 1061, 2271, 2415, 2561
BfmI CTRYAG 2 cut(s) 376, 1240
BfrI CTTAAG 2 cut(s) 812, 1537
BfuI GTATCC 1 cut(s) 2473
BisI GCNGC 5 cut(s) 155, 504, 757, 1276, 1985
BlpI GCTNAGC 1 cut(s) 150
BlsI GCNGC 5 cut(s) 156, 505, 758, 1277, 1986
Bme1390I CCNGG 5 cut(s) 230, 942, 2042, 2343, 2582
Bme18I GGWCC 2 cut(s) 314, 1231
BmeRI GACNNNNNGTC 1 cut(s) 178
BmeT110I CYCGRG 1 cut(s) 1785
BmgBI CACGTC 1 cut(s) 1967
BmgT120I GGNCC 4 cut(s) 314, 1231, 1484, 1873
BmiI GGNNCC 6 cut(s) 167, 316, 360, 946, 1705, 1942
BmrFI CCNGG 5 cut(s) 230, 942, 2042, 2343, 2582
BmrI ACTGGG 3 cut(s) 591, 625, 940
BmsI GCATC 6 cut(s) 257, 374, 882, 1066, 1447, 1632
BmuI ACTGGG 3 cut(s) 591, 625, 940
BpiI GAAGAC 4 cut(s) 230, 252, 1143, 2328
BplI GAGNNNNNCTC 2 cut(s) 1454, 1486
BpmI CTGGAG 1 cut(s) 1002
Bpu1102I GCTNAGC 1 cut(s) 150
BpuEI CTTGAG 1 cut(s) 1675
BsaBI GATNNNNATC 1 cut(s) 722
BsaI GGTCTC 2 cut(s) 881, 2237
BsaJI CCNNGG 7 cut(s) 228, 327, 439, 664, 948, 2247, 2466
Bsc4I CCNNNNNNNGG 6 cut(s) 235, 445, 616, 1321, 1881, 2405
Bse118I RCCGGY 2 cut(s) 807, 1790
Bse1I ACTGG 8 cut(s) 597, 620, 760, 886, 935, 1726, 2410, 2443
Bse21I CCTNAGG 1 cut(s) 161
Bse3DI GCAATG 2 cut(s) 1035, 1995
Bse8I GATNNNNATC 1 cut(s) 722
BseBI CCWGG 5 cut(s) 230, 942, 2042, 2343, 2582
BseDI CCNNGG 7 cut(s) 228, 327, 439, 664, 948, 2247, 2466
BseGI GGATG 6 cut(s) 272, 365, 1209, 1438, 1647, 2231
BseJI GATNNNNATC 1 cut(s) 722
BseLI CCNNNNNNNGG 6 cut(s) 235, 445, 616, 1321, 1881, 2405
BseMI GCAATG 2 cut(s) 1035, 1995
BseMII CTCAG 4 cut(s) 152, 164, 2232, 2247
BseNI ACTGG 8 cut(s) 597, 620, 760, 886, 935, 1726, 2410, 2443
BseRI GAGGAG 5 cut(s) 43, 251, 1245, 2544, 2547
BseXI GCAGC 5 cut(s) 166, 490, 768, 1262, 1996
Bsh1236I CGCG 1 cut(s) 12
BshFI GGCC 4 cut(s) 1002, 1309, 1485, 1874
BsiHKAI GWGCWC 1 cut(s) 1573
BsiHKCI CYCGRG 1 cut(s) 1785
BsiSI CCGG 2 cut(s) 808, 1791
BslFI GGGAC 5 cut(s) 82, 289, 480, 2163, 2368
BslI CCNNNNNNNGG 6 cut(s) 235, 445, 616, 1321, 1881, 2405
BsmAI GTCTC 8 cut(s) 9, 147, 186, 881, 1734, 2014, 2237, 2498
BsmBI CGTCTC 2 cut(s) 147, 2014
BsmFI GGGAC 5 cut(s) 82, 289, 480, 2163, 2368
BsmI GAATGC 1 cut(s) 494
BsnI GGCC 4 cut(s) 1002, 1309, 1485, 1874
Bso31I GGTCTC 2 cut(s) 881, 2237
BsoBI CYCGRG 1 cut(s) 1785
Bsp1286I GDGCHC 1 cut(s) 1573
Bsp1407I TGTACA 1 cut(s) 1974
Bsp143I GATC 7 cut(s) 304, 944, 1007, 1250, 1840, 2095, 2470
Bsp1720I GCTNAGC 1 cut(s) 150
Bsp19I CCATGG 3 cut(s) 439, 664, 2466
BspACI CCGC 6 cut(s) 10, 206, 212, 1033, 1177, 1924
BspANI GGCC 4 cut(s) 1002, 1309, 1485, 1874
BspCNI CTCAG 4 cut(s) 153, 163, 2233, 2248
BspFNI CGCG 1 cut(s) 12
BspLI GGNNCC 6 cut(s) 167, 316, 360, 946, 1705, 1942
BspMAI CTGCAG 1 cut(s) 380
BspPI GGATC 5 cut(s) 939, 952, 1258, 1835, 2478
BspTI CTTAAG 2 cut(s) 812, 1537
BspTNI GGTCTC 2 cut(s) 881, 2237
BsrDI GCAATG 2 cut(s) 1035, 1995
BsrFI RCCGGY 2 cut(s) 807, 1790
BsrGI TGTACA 1 cut(s) 1974
BsrI ACTGG 8 cut(s) 597, 620, 760, 886, 935, 1726, 2410, 2443
BssAI RCCGGY 2 cut(s) 807, 1790
BssECI CCNNGG 7 cut(s) 228, 327, 439, 664, 948, 2247, 2466
BssMI GATC 7 cut(s) 304, 944, 1007, 1250, 1840, 2095, 2470
BssSI CACGAG 1 cut(s) 727
BssT1I CCWWGG 5 cut(s) 327, 439, 664, 948, 2466
Bst2BI CACGAG 1 cut(s) 727
Bst2UI CCWGG 5 cut(s) 230, 942, 2042, 2343, 2582
Bst4CI ACNGT 2 cut(s) 72, 1554
Bst6I CTCTTC 1 cut(s) 189
BstAFI CTTAAG 2 cut(s) 812, 1537
BstAPI GCANNNNNTGC 1 cut(s) 1466
BstAUI TGTACA 1 cut(s) 1974
BstC8I GCNNGC 1 cut(s) 1569
BstDEI CTNAG 6 cut(s) 150, 161, 633, 2241, 2256, 2502
BstDSI CCRYGG 4 cut(s) 439, 664, 2247, 2466
BstF5I GGATG 6 cut(s) 272, 365, 1209, 1438, 1647, 2231
BstFNI CGCG 1 cut(s) 12
BstHHI GCGC 2 cut(s) 14, 1725
BstKTI GATC 7 cut(s) 307, 947, 1010, 1253, 1843, 2098, 2473
BstMAI GTCTC 8 cut(s) 9, 147, 186, 881, 1734, 2014, 2237, 2498
BstMBI GATC 7 cut(s) 304, 944, 1007, 1250, 1840, 2095, 2470
BstMWI GCNNNNNNNGC 7 cut(s) 163, 500, 1054, 1417, 1466, 1906, 2143
BstNI CCWGG 5 cut(s) 230, 942, 2042, 2343, 2582
BstNSI RCATGY 3 cut(s) 846, 1087, 1913
BstSCI CCNGG 5 cut(s) 228, 940, 2040, 2341, 2580
BstSFI CTRYAG 2 cut(s) 376, 1240
BstUI CGCG 1 cut(s) 12
BstV1I GCAGC 5 cut(s) 166, 490, 768, 1262, 1996
BstV2I GAAGAC 4 cut(s) 230, 252, 1143, 2328
BstX2I RGATCY 2 cut(s) 944, 1250
BstYI RGATCY 2 cut(s) 944, 1250
Bsu36I CCTNAGG 1 cut(s) 161
BsuI GTATCC 1 cut(s) 2473
BsuRI GGCC 4 cut(s) 1002, 1309, 1485, 1874
BtgI CCRYGG 4 cut(s) 439, 664, 2247, 2466
BtrI CACGTC 1 cut(s) 1967
BtsCI GGATG 6 cut(s) 272, 365, 1209, 1438, 1647, 2231
BtsIMutI CAGTG 4 cut(s) 879, 1559, 1822, 2403
Cac8I GCNNGC 1 cut(s) 1569
CaiI CAGNNNCTG 1 cut(s) 160
CfoI GCGC 2 cut(s) 14, 1725
Cfr10I RCCGGY 2 cut(s) 807, 1790
Cfr13I GGNCC 4 cut(s) 314, 1231, 1484, 1873
Csp6I GTAC 3 cut(s) 1289, 1975, 2436
CspCI CAANNNNNGTGG 2 cut(s) 2392, 2427
CviQI GTAC 3 cut(s) 1289, 1975, 2436
DdeI CTNAG 6 cut(s) 150, 161, 633, 2241, 2256, 2502
DpnI GATC 7 cut(s) 306, 946, 1009, 1252, 1842, 2097, 2472
DpnII GATC 7 cut(s) 304, 944, 1007, 1250, 1840, 2095, 2470
DraI TTTAAA 1 cut(s) 856
DraIII CACNNNGTG 1 cut(s) 1607
DriI GACNNNNNGTC 1 cut(s) 178
EaeI YGGCCR 2 cut(s) 1000, 1307
Eam1104I CTCTTC 1 cut(s) 189
Eam1105I GACNNNNNGTC 1 cut(s) 178
EarI CTCTTC 1 cut(s) 189
EciI GGCGGA 1 cut(s) 1913
Eco130I CCWWGG 5 cut(s) 327, 439, 664, 948, 2466
Eco31I GGTCTC 2 cut(s) 881, 2237
Eco47I GGWCC 2 cut(s) 314, 1231
Eco81I CCTNAGG 1 cut(s) 161
Eco88I CYCGRG 1 cut(s) 1785
EcoO109I RGGNCCY 2 cut(s) 314, 1231
EcoRI GAATTC 2 cut(s) 626, 2481
EcoRII CCWGG 5 cut(s) 228, 940, 2040, 2341, 2580
EcoT14I CCWWGG 5 cut(s) 327, 439, 664, 948, 2466
ErhI CCWWGG 5 cut(s) 327, 439, 664, 948, 2466
Esp3I CGTCTC 2 cut(s) 147, 2014
FalI AAGNNNNNCTT 4 cut(s) 12, 44, 2052, 2084
FaqI GGGAC 5 cut(s) 82, 289, 480, 2163, 2368
FauI CCCGC 1 cut(s) 1170
FbaI TGATCA 1 cut(s) 304
FblI GTMKAC 1 cut(s) 59
Fnu4HI GCNGC 5 cut(s) 155, 504, 757, 1276, 1985
FokI GGATG 6 cut(s) 279, 352, 1196, 1445, 1654, 2218
Fsp4HI GCNGC 5 cut(s) 155, 504, 757, 1276, 1985
FspBI CTAG 9 cut(s) 123, 218, 279, 470, 848, 1061, 2271, 2415, 2561
GlaI GCGC 2 cut(s) 13, 1724
GluI GCNGC 5 cut(s) 155, 504, 757, 1276, 1985
GsuI CTGGAG 1 cut(s) 1002
HaeIII GGCC 4 cut(s) 1002, 1309, 1485, 1874
HapII CCGG 2 cut(s) 808, 1791
HhaI GCGC 2 cut(s) 14, 1725
Hin6I GCGC 2 cut(s) 12, 1723
HinP1I GCGC 2 cut(s) 12, 1723
HincII GTYRAC 3 cut(s) 60, 1738, 2276
HindII GTYRAC 3 cut(s) 60, 1738, 2276
HinfI GANTC 7 cut(s) 536, 1343, 1807, 1852, 2186, 2252, 2367
HpaII CCGG 2 cut(s) 808, 1791
HphI GGTGA 2 cut(s) 536, 1619
Hpy166II GTNNAC 5 cut(s) 60, 661, 1738, 1975, 2276
Hpy188I TCNGA 7 cut(s) 396, 549, 769, 1428, 2104, 2242, 2257
Hpy8I GTNNAC 5 cut(s) 60, 661, 1738, 1975, 2276
Hpy99I CGWCG 6 cut(s) 61, 79, 82, 85, 88, 2430
HpyCH4III ACNGT 2 cut(s) 72, 1554
HpyCH4IV ACGT 1 cut(s) 1966
HpyF10VI GCNNNNNNNGC 7 cut(s) 163, 500, 1054, 1417, 1466, 1906, 2143
HpyF3I CTNAG 6 cut(s) 150, 161, 633, 2241, 2256, 2502
HpySE526I ACGT 1 cut(s) 1966
HspAI GCGC 2 cut(s) 12, 1723
Ksp22I TGATCA 1 cut(s) 304
Kzo9I GATC 7 cut(s) 304, 944, 1007, 1250, 1840, 2095, 2470
LmnI GCTCC 7 cut(s) 162, 171, 961, 1021, 1389, 1408, 2557
Lsp1109I GCAGC 5 cut(s) 166, 490, 768, 1262, 1996
LweI GCATC 6 cut(s) 257, 374, 882, 1066, 1447, 1632
MaeI CTAG 9 cut(s) 123, 218, 279, 470, 848, 1061, 2271, 2415, 2561
MaeII ACGT 1 cut(s) 1966
MaeIII GTNAC 4 cut(s) 1194, 1397, 1628, 2221
MalI GATC 7 cut(s) 306, 946, 1009, 1252, 1842, 2097, 2472
MboI GATC 7 cut(s) 304, 944, 1007, 1250, 1840, 2095, 2470
MboII GAAGA 7 cut(s) 176, 235, 257, 1148, 2220, 2333, 2617
MfeI CAATTG 1 cut(s) 489
MflI RGATCY 2 cut(s) 944, 1250
MhlI GDGCHC 1 cut(s) 1573
MlsI TGGCCA 2 cut(s) 1002, 1309
MluNI TGGCCA 2 cut(s) 1002, 1309
MlyI GAGTC 1 cut(s) 2376
MmeI TCCRAC 1 cut(s) 1411
Mox20I TGGCCA 2 cut(s) 1002, 1309
MroXI GAANNNNTTC 1 cut(s) 1662
MscI TGGCCA 2 cut(s) 1002, 1309
MslI CAYNNNNRTG 3 cut(s) 265, 2316, 2513
Msp20I TGGCCA 2 cut(s) 1002, 1309
MspCI CTTAAG 2 cut(s) 812, 1537
MspI CCGG 2 cut(s) 808, 1791
MspR9I CCNGG 5 cut(s) 230, 942, 2042, 2343, 2582
MunI CAATTG 1 cut(s) 489
Mva1269I GAATGC 1 cut(s) 494
MvaI CCWGG 5 cut(s) 230, 942, 2042, 2343, 2582
MvnI CGCG 1 cut(s) 12
MwoI GCNNNNNNNGC 7 cut(s) 163, 500, 1054, 1417, 1466, 1906, 2143
NcoI CCATGG 3 cut(s) 439, 664, 2466
NdeII GATC 7 cut(s) 304, 944, 1007, 1250, 1840, 2095, 2470
NlaIV GGNNCC 6 cut(s) 167, 316, 360, 946, 1705, 1942
NmuCI GTSAC 1 cut(s) 2221
NspI RCATGY 3 cut(s) 846, 1087, 1913
PaeR7I CTCGAG 1 cut(s) 1785
PciI ACATGT 1 cut(s) 1083
PcsI WCGNNNNNNNCGW 2 cut(s) 80, 83
PctI GAATGC 1 cut(s) 494
PdmI GAANNNNTTC 1 cut(s) 1662
PfeI GAWTC 6 cut(s) 536, 1343, 1807, 1852, 2186, 2252
PflFI GACNNNGTC 1 cut(s) 1142
PfoI TCCNGGA 2 cut(s) 2040, 2580
PkrI GCNGC 5 cut(s) 156, 505, 758, 1277, 1986
PleI GAGTC 1 cut(s) 2375
PpsI GAGTC 1 cut(s) 2375
PpuMI RGGWCCY 2 cut(s) 314, 1231
PscI ACATGT 1 cut(s) 1083
PsiI TTATAA 1 cut(s) 2632
Psp5II RGGWCCY 2 cut(s) 314, 1231
Psp6I CCWGG 5 cut(s) 228, 940, 2040, 2341, 2580
PspGI CCWGG 5 cut(s) 228, 940, 2040, 2341, 2580
PspN4I GGNNCC 6 cut(s) 167, 316, 360, 946, 1705, 1942
PspPI GGNCC 4 cut(s) 314, 1231, 1484, 1873
PspPPI RGGWCCY 2 cut(s) 314, 1231
PspXI VCTCGAGB 1 cut(s) 1785
PstI CTGCAG 1 cut(s) 380
PstNI CAGNNNCTG 1 cut(s) 160
PsuI RGATCY 2 cut(s) 944, 1250
PsyI GACNNNGTC 1 cut(s) 1142
RsaI GTAC 3 cut(s) 1290, 1976, 2437
RsaNI GTAC 3 cut(s) 1289, 1975, 2436
RseI CAYNNNNRTG 3 cut(s) 265, 2316, 2513
SalI GTCGAC 1 cut(s) 58
SatI GCNGC 5 cut(s) 155, 504, 757, 1276, 1985
Sau3AI GATC 7 cut(s) 304, 944, 1007, 1250, 1840, 2095, 2470
Sau96I GGNCC 4 cut(s) 314, 1231, 1484, 1873
SchI GAGTC 1 cut(s) 2376
ScrFI CCNGG 5 cut(s) 230, 942, 2042, 2343, 2582
SduI GDGCHC 1 cut(s) 1573
SfaNI GCATC 6 cut(s) 257, 374, 882, 1066, 1447, 1632
SfcI CTRYAG 2 cut(s) 376, 1240
Sfr274I CTCGAG 1 cut(s) 1785
SinI GGWCC 2 cut(s) 314, 1231
SlaI CTCGAG 1 cut(s) 1785
SmiMI CAYNNNNRTG 3 cut(s) 265, 2316, 2513
SmlI CTYRAG 4 cut(s) 812, 1537, 1690, 1785
SmoI CTYRAG 4 cut(s) 812, 1537, 1690, 1785
SpeI ACTAGT 1 cut(s) 278
SsiI CCGC 6 cut(s) 10, 206, 212, 1033, 1177, 1924
SspI AATATT 1 cut(s) 1836
SspMI CTAG 9 cut(s) 123, 218, 279, 470, 848, 1061, 2271, 2415, 2561
StyD4I CCNGG 5 cut(s) 228, 940, 2040, 2341, 2580
StyI CCWWGG 5 cut(s) 327, 439, 664, 948, 2466
TaaI ACNGT 2 cut(s) 72, 1554
TaiI ACGT 1 cut(s) 1969
TaqI TCGA 3 cut(s) 59, 795, 1786
TatI WGTACW 1 cut(s) 1974
TfiI GAWTC 6 cut(s) 536, 1343, 1807, 1852, 2186, 2252
TscAI CASTG 4 cut(s) 886, 1559, 1822, 2410
TseFI GTSAC 1 cut(s) 2221
TseI GCWGC 5 cut(s) 154, 503, 756, 1275, 1984
Tsp45I GTSAC 1 cut(s) 2221
TspDTI ATGAA 9 cut(s) 336, 834, 999, 1335, 1965, 2154, 2220, 2334, 2497
TspGWI ACGGA 1 cut(s) 2264
TspRI CASTG 4 cut(s) 886, 1559, 1822, 2410
Tth111I GACNNNGTC 1 cut(s) 1142
Vha464I CTTAAG 2 cut(s) 812, 1537
VpaK11BI GGWCC 2 cut(s) 314, 1231
XapI RAATTY 7 cut(s) 626, 747, 851, 1661, 2076, 2481, 2528
XbaI TCTAGA 2 cut(s) 847, 2560
XceI RCATGY 3 cut(s) 846, 1087, 1913
XcmI CCANNNNNNNNNTGG 1 cut(s) 142
XhoI CTCGAG 1 cut(s) 1785
XmiI GTMKAC 1 cut(s) 59
XmnI GAANNNNTTC 1 cut(s) 1662
XspI CTAG 9 cut(s) 123, 218, 279, 470, 848, 1061, 2271, 2415, 2561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.