RLG00000018935

DNA excision repair protein ERCC-6-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0013205
Physical Location & Seq
Forward (+)
43108625 .. 43115994
7370 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018935

Sequence Viewer

Length: 2889 bp
ATGTCTCTCCGCGCCTTCAAAGAGAGCCTTAAACCCTGCCAAACCCAAAACTCCTCGTCGACCCAAACACAGTCACAGTCGTCGTCGTCGTCGTCAATTCTGTCCCAAATCCCAGAACCCACAATCCCTAGAAAGCCCCCCAAATCGTCTCTGGCTCAGCAGCTCCTGAGACTCCAAGACCCTCTGTCTCTTCAACCACCTCCACCTCCACCTCCACCTCCACCACCGCCCCTAGAGAAGACCCAAATCAAACAAACCCAGGTGGAGGATCCCGATGAGGAGGACGAAAACGACCCAGAACCACTGGGATGCTCTGGGACTAGTAAAGTTAGTAGCTTTCAGTTTGATCACACAGGACCCTATGAACCCTTGGTCTTATCCCATTTTCCTCTCATTCAGGTTCCTGCATCCATTAACTGCAGATTGCTTCCACATCAGAGAGAGGGAGTAAAGTTTCTGTACAATATATACAAGAACAACCATGGAGGGGTTCTTGGAGATGACATGGGGCTAGGCAAAACCATTCAGACAATTGCATTCTTGGCTGCTGTGTTTGGGAAAGATGGTGATCGCATTGATTCCACAATTCCAAAGAGAAGTGAAATAGCAGAACGAAACCCTGTTCTCATAGTTTGCCCCAGTTCAGTTATCCACAACTGGGAGAGCGAATTCTCTAAGTGGTCTAACTTTAGTGTTGCTGTTTACCATGGTGCAAATCGTGATTTGATTTATGATAAATTGGAAGCTCATGGAGTTGAGATACTTATCACGAGCTTTGACACATACAGAATTTGTGGCAGCCAGTTATCAGATGTTAATTGGGAGATTGTTGTTGTCGATGAGGCACACCGGCTTAAGAATGAAAAATCAAAGCTCTACATAGCATGTCTAGAATTTAAAACCTTAAAACGAATTGGTCTCACTGGAACTATGATGCAGAACAAAATCATGGAATTGTTTAACCTGTTTGACTGGGTTGCACCTGGATCCTTGGGAACACGGGAGCATTTTCGGGAGTTTTATGATGAACCTCTCAAACATGGCCAAAGATCAACTGCTCCAGAAAGATTTGTCCGCATTGCTGATGAGCGGAAACAGCATCTAGTAGCTGTTCTTAATAAATATATGTTGAGAAGGACAAAAGAGGAAACCATTGGTCATCTTATGATGGGGAAGGAAGACAATGTCATATTTTGTTCAATGAGCGAATCGCAAAAGCGGGTGTATAGGCGAATGTTACAGCTACCAGACATCCAATGCCTGATAAATAAGGACCTTCCCTGTAGTTGTGGATCTCCTCTTACCCAAGTTGAGTGCTGCAAAAGGATTGTACCGGATGGAAAAATATGGCCATACCTTCACAGGGACAACCCTGATGGTTGTGATTCATGCCCATTTTGTATTGTCCTTCCTTGCCTTGTCAAGCTCCAACAGATAAGTAACCACTTGGAGCTTATTAAGCCTAATCCGAAGGATGACCCAGAAAAGCAAAAGAAAGATGCAGAGTTTGCCTCTGCTGTGTTTGGCCCAGATATTGATTTGGTGGGAGGCAACACTCAAAATGAGAGTTTTATGGGCTTGAGTGATGTCAAACAGTGTGGGAAAATGCGAGCACTTGAAAAGTTTATGTTCTCATGGATTGCACAGGGAGATAAAGTCCTTCTGTTTAGTTACTCTGTCAGGATGTTGGACATATTGGAAAAATTTCTTATACGCAAAGGTTATTGCTTCTCGAGACTTGATGGTTCTACACCAACCAACTTGCGCCAGTCTCTTGTCGACAACTTTAATTCAAGTCCAAGCAAACAGGTGTTCCTTATATCAACTCGTGCCGGTGGGCTTGGACTGAATCTTGTCAGCGCAAATCGTGTTGTAATATTTGATCCAAGCTGGAATCCTGCCCAAGACTTACAGGCCCAAGACAGGTCATTTCGGTTCGGGCAGAAACGGCATGTTGTGGTTTTCCGCCTTCTTTCTGCTGGTTCCCTTGATGAACTTGTTTATTCACGTCAGGTGTACAAACAGCAGCTGTCAAACATTGCTGTTTCTGGAAAAATGGAGAAACGATATTTTGAAGGTGTCCAGGATTGCAAAGACTTTCAAGGGGAACTTTTTGGAATTTGCAATTTATTTCGTGACCTTTCAGATAAGCTCTTTACCAGCGAAATATTTGAGTTGCATGAAAAGCAGGGACAAAAAGAAGGGGGTTGTCATGGTTGGGAACAAGAATCAACCAAAATTACTTGCATAAAAGAAGTGGGTGTGACATCTTCATCCGTGTCTGAGACCAATGATTCTGAGAAGGCTTTAGCTAGTCAACCTGTGCTCAAACATGTGGGTATTGTCTATGCACATCGTAATGAAGACATTGTGAATAATAGACCTGGAATGCAAGGGACGATGGAAATAGTCATTCCTTGTAATGATAGCCTAAAACAGCCCCCCACTGGTGATGCTAGGAGAAAGCGACGAGAGGTTACTGGTGGAAAGGAGAATGTATCTGTATCCATGGATCGCAAAAGGATTCAATTTAGTCTCCTTGCTAAGTTCATGGGTATGGGAGAGATTGAATTTAGCAAGTGGGTAGTATCTGCTTCTCCTCTGGAGAGGGAAAAAGTTCTCCAGGACTACAAGGAGAGAAACAAGAAGAAACTCAACAGCCAAAACGAACCTGAATGGCACAGCGACGACGGCCTGGCCTTTATGGTTGACGAGCTCTGCAGGCTACTAGTTGAGGAACTCCGGCTTGAGGTCCCCTTCGTGACGTTTCAATCGTGTTACTGTTGGTTGACGAGCTCCGCACGCTACGCCGAGAAGCCGCTGGACGCCTTGAAGGAGATTCAGTCGCTTGTCCTGCCAGTGAGCTTTGGCCTTTTGCTATCGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helicase_C SNF2_N

Protein Analysis

963

Amino Acids

108.8

Weight (kDa)

6.86

Isoelectric Point (pI)

47.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 140 - 311 9.4e-09 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 145 - 419 3.5e-65 SNF2-related domain
DEAD PF00270 164 - 314 6.3e-06 DEAD/DEAH box helicase
Helicase_C PF00271 535 - 647 2.7e-23 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013205)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03750
fragaria_vesca FvH4_6g26480 FvH4_6g26480 FvH4_6g26480 FvH4_6g26480
malus_domestica MD17G1236900.v1.1
prunus_persica Prupe.3G139100_v2.0.a1
pyrus_communis pycom17g24160
rosa_chinensis RchiOBHm_Chr2g0127761
rosa_laevigata RLG00000018935
rosa_multiflora Rmu_sc0001829.1_g000008 Rmu_sc0004240.1_g000009
rosa_roxburghii Rroxscaffold_2G00116780
rosa_rugosa Rorug02G0271200
rosa_samantha Rh2AG323300 Rh2BG332500 Rh2CG311100
rosa_wichuraiana Rw2G026240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1090
AccI GTMKAC 2 cut(s) 59, 1779
AccII CGCG 1 cut(s) 12
AciI CCGC 8 cut(s) 10, 227, 1075, 1090, 1219, 1966, 2799, 2819
AclWI GGATC 7 cut(s) 263, 276, 981, 994, 1300, 1877, 2520
AcoI YGGCCR 2 cut(s) 1042, 1349
AcsI RAATTY 6 cut(s) 668, 789, 893, 1703, 2118, 2570
AcyI GRCGYC 1 cut(s) 2826
AfaI GTAC 3 cut(s) 461, 1332, 2018
AfiI CCNNNNNNNGG 7 cut(s) 265, 487, 658, 1363, 1923, 2447, 2749
AflII CTTAAG 1 cut(s) 854
AflIII ACRYGT 1 cut(s) 2332
AhdI GACNNNNNGTC 1 cut(s) 184
AhlI ACTAGT 2 cut(s) 320, 2728
AjiI CACGTC 1 cut(s) 2009
AjnI CCWGG 6 cut(s) 258, 982, 2082, 2383, 2622, 2694
AloI GAACNNNNNNTCC 2 cut(s) 108, 140
Alw21I GWGCWC 4 cut(s) 1615, 2328, 2718, 2798
Alw26I GTCTC 9 cut(s) 9, 153, 163, 192, 923, 1729, 1776, 2279, 2540
AlwI GGATC 7 cut(s) 263, 276, 981, 994, 1300, 1877, 2520
AlwNI CAGNNNCTG 2 cut(s) 166, 2029
Ama87I CYCGRG 1 cut(s) 1732
AoxI GGCC 7 cut(s) 1042, 1349, 1525, 1914, 2692, 2697, 2869
ApeKI GCWGC 5 cut(s) 160, 545, 798, 1317, 2026
ApoI RAATTY 6 cut(s) 668, 789, 893, 1703, 2118, 2570
Asp700I GAANNNNTTC 2 cut(s) 1704, 2616
AspLEI GCGC 3 cut(s) 14, 1767, 1862
AspS9I GGNCC 5 cut(s) 356, 1273, 1526, 1915, 2752
AsuHPI GGTGA 2 cut(s) 578, 2462
AvaI CYCGRG 1 cut(s) 1732
AvaII GGWCC 3 cut(s) 356, 1273, 2752
BalI TGGCCA 2 cut(s) 1044, 1351
BamHI GGATCC 2 cut(s) 268, 986
BanII GRGCYC 2 cut(s) 2718, 2798
BauI CACGAG 2 cut(s) 769, 1827
BbsI GAAGAC 3 cut(s) 245, 1185, 2370
Bbv12I GWGCWC 4 cut(s) 1615, 2328, 2718, 2798
BbvI GCAGC 5 cut(s) 172, 532, 810, 1304, 2038
BccI CCATC 6 cut(s) 557, 1162, 1331, 1370, 1736, 2395
BceAI ACGGC 2 cut(s) 1964, 2707
BciT130I CCWGG 6 cut(s) 260, 984, 2084, 2385, 2624, 2696
BciVI GTATCC 1 cut(s) 2515
BclI TGATCA 1 cut(s) 346
BcoDI GTCTC 9 cut(s) 9, 153, 163, 192, 923, 1729, 1776, 2279, 2540
BcuI ACTAGT 2 cut(s) 320, 2728
BfaI CTAG 9 cut(s) 129, 233, 321, 512, 890, 1103, 2313, 2457, 2729
BfmI CTRYAG 3 cut(s) 418, 1282, 2719
BfrI CTTAAG 1 cut(s) 854
BfuI GTATCC 1 cut(s) 2515
BisI GCNGC 6 cut(s) 161, 546, 799, 1318, 2027, 2819
BlpI GCTNAGC 1 cut(s) 156
BlsI GCNGC 6 cut(s) 162, 547, 800, 1319, 2028, 2820
Bme1390I CCNGG 6 cut(s) 260, 984, 2084, 2385, 2624, 2696
Bme18I GGWCC 3 cut(s) 356, 1273, 2752
BmeRI GACNNNNNGTC 1 cut(s) 184
BmeT110I CYCGRG 1 cut(s) 1732
BmgBI CACGTC 1 cut(s) 2009
BmgT120I GGNCC 5 cut(s) 356, 1273, 1526, 1915, 2752
BmiI GGNNCC 6 cut(s) 270, 358, 402, 988, 1984, 2754
BmrFI CCNGG 6 cut(s) 260, 984, 2084, 2385, 2624, 2696
BmrI ACTGGG 4 cut(s) 314, 633, 667, 982
BmsI GCATC 6 cut(s) 299, 416, 924, 1108, 1489, 2443
BmuI ACTGGG 4 cut(s) 314, 633, 667, 982
BpiI GAAGAC 3 cut(s) 245, 1185, 2370
BplI GAGNNNNNCTC 2 cut(s) 1496, 1528
BpmI CTGGAG 3 cut(s) 1044, 2606, 2624
Bpu1102I GCTNAGC 1 cut(s) 156
BpuEI CTTGAG 2 cut(s) 1600, 2768
BsaBI GATNNNNATC 2 cut(s) 567, 764
BsaHI GRCGYC 1 cut(s) 2826
BsaI GGTCTC 2 cut(s) 923, 2279
BsaJI CCNNGG 6 cut(s) 258, 369, 481, 706, 990, 2508
BsaWI WCCGGW 1 cut(s) 1333
BsaXI ACNNNNNCTCC 4 cut(s) 1641, 1671, 2828, 2858
Bsc4I CCNNNNNNNGG 7 cut(s) 265, 487, 658, 1363, 1923, 2447, 2749
Bse118I RCCGGY 2 cut(s) 849, 1832
Bse3DI GCAATG 2 cut(s) 1077, 2037
Bse8I GATNNNNATC 2 cut(s) 567, 764
BseBI CCWGG 6 cut(s) 260, 984, 2084, 2385, 2624, 2696
BseDI CCNNGG 6 cut(s) 258, 369, 481, 706, 990, 2508
BseGI GGATG 7 cut(s) 314, 407, 1251, 1342, 1480, 1689, 2273
BseJI GATNNNNATC 2 cut(s) 567, 764
BseLI CCNNNNNNNGG 7 cut(s) 265, 487, 658, 1363, 1923, 2447, 2749
BseMI GCAATG 2 cut(s) 1077, 2037
BseMII CTCAG 4 cut(s) 158, 170, 2274, 2289
BseRI GAGGAG 4 cut(s) 43, 293, 1287, 2589
BseXI GCAGC 5 cut(s) 172, 532, 810, 1304, 2038
Bsh1236I CGCG 1 cut(s) 12
BshFI GGCC 7 cut(s) 1044, 1351, 1527, 1916, 2694, 2699, 2871
BsiHKAI GWGCWC 4 cut(s) 1615, 2328, 2718, 2798
BsiHKCI CYCGRG 1 cut(s) 1732
BsiSI CCGG 4 cut(s) 850, 1334, 1833, 2743
BslFI GGGAC 6 cut(s) 88, 331, 1379, 2205, 2410, 2738
BslI CCNNNNNNNGG 7 cut(s) 265, 487, 658, 1363, 1923, 2447, 2749
BsmAI GTCTC 9 cut(s) 9, 153, 163, 192, 923, 1729, 1776, 2279, 2540
BsmBI CGTCTC 1 cut(s) 153
BsmFI GGGAC 6 cut(s) 88, 331, 1379, 2205, 2410, 2738
BsmI GAATGC 2 cut(s) 536, 2394
BsnI GGCC 7 cut(s) 1044, 1351, 1527, 1916, 2694, 2699, 2871
Bso31I GGTCTC 2 cut(s) 923, 2279
BsoBI CYCGRG 1 cut(s) 1732
Bsp1286I GDGCHC 4 cut(s) 1615, 2328, 2718, 2798
Bsp1407I TGTACA 2 cut(s) 459, 2016
Bsp143I GATC 8 cut(s) 268, 346, 568, 986, 1049, 1292, 1882, 2512
Bsp1720I GCTNAGC 1 cut(s) 156
Bsp19I CCATGG 3 cut(s) 481, 706, 2508
BspACI CCGC 8 cut(s) 10, 227, 1075, 1090, 1219, 1966, 2799, 2819
BspANI GGCC 7 cut(s) 1044, 1351, 1527, 1916, 2694, 2699, 2871
BspCNI CTCAG 4 cut(s) 159, 169, 2275, 2290
BspFNI CGCG 1 cut(s) 12
BspLI GGNNCC 6 cut(s) 270, 358, 402, 988, 1984, 2754
BspMAI CTGCAG 2 cut(s) 422, 2723
BspPI GGATC 7 cut(s) 263, 276, 981, 994, 1300, 1877, 2520
BspTI CTTAAG 1 cut(s) 854
BspTNI GGTCTC 2 cut(s) 923, 2279
BsrBI CCGCTC 1 cut(s) 1090
BsrDI GCAATG 2 cut(s) 1077, 2037
BsrFI RCCGGY 2 cut(s) 849, 1832
BsrGI TGTACA 2 cut(s) 459, 2016
BssAI RCCGGY 2 cut(s) 849, 1832
BssECI CCNNGG 6 cut(s) 258, 369, 481, 706, 990, 2508
BssMI GATC 8 cut(s) 268, 346, 568, 986, 1049, 1292, 1882, 2512
BssNI GRCGYC 1 cut(s) 2826
BssSI CACGAG 2 cut(s) 769, 1827
BssT1I CCWWGG 5 cut(s) 369, 481, 706, 990, 2508
Bst2BI CACGAG 2 cut(s) 769, 1827
Bst2UI CCWGG 6 cut(s) 260, 984, 2084, 2385, 2624, 2696
Bst4CI ACNGT 4 cut(s) 72, 78, 1596, 2783
Bst6I CTCTTC 1 cut(s) 195
BstACI GRCGYC 1 cut(s) 2826
BstAFI CTTAAG 1 cut(s) 854
BstAPI GCANNNNNTGC 1 cut(s) 1508
BstAUI TGTACA 2 cut(s) 459, 2016
BstC8I GCNNGC 3 cut(s) 1611, 2723, 2803
BstDEI CTNAG 6 cut(s) 156, 167, 675, 2283, 2298, 2544
BstDSI CCRYGG 3 cut(s) 481, 706, 2508
BstF5I GGATG 7 cut(s) 314, 407, 1251, 1342, 1480, 1689, 2273
BstFNI CGCG 1 cut(s) 12
BstHHI GCGC 3 cut(s) 14, 1767, 1862
BstKTI GATC 8 cut(s) 271, 349, 571, 989, 1052, 1295, 1885, 2515
BstMAI GTCTC 9 cut(s) 9, 153, 163, 192, 923, 1729, 1776, 2279, 2540
BstMBI GATC 8 cut(s) 268, 346, 568, 986, 1049, 1292, 1882, 2512
BstNI CCWGG 6 cut(s) 260, 984, 2084, 2385, 2624, 2696
BstNSI RCATGY 3 cut(s) 888, 1955, 2336
BstSCI CCNGG 6 cut(s) 258, 982, 2082, 2383, 2622, 2694
BstSFI CTRYAG 3 cut(s) 418, 1282, 2719
BstUI CGCG 1 cut(s) 12
BstV1I GCAGC 5 cut(s) 172, 532, 810, 1304, 2038
BstV2I GAAGAC 3 cut(s) 245, 1185, 2370
BstX2I RGATCY 3 cut(s) 268, 986, 1292
BstYI RGATCY 3 cut(s) 268, 986, 1292
BsuI GTATCC 1 cut(s) 2515
BsuRI GGCC 7 cut(s) 1044, 1351, 1527, 1916, 2694, 2699, 2871
BtgI CCRYGG 3 cut(s) 481, 706, 2508
BtrI CACGTC 1 cut(s) 2009
BtsCI GGATG 7 cut(s) 314, 407, 1251, 1342, 1480, 1689, 2273
BtsIMutI CAGTG 5 cut(s) 302, 921, 1601, 2445, 2865
Cac8I GCNNGC 3 cut(s) 1611, 2723, 2803
CaiI CAGNNNCTG 2 cut(s) 166, 2029
CfoI GCGC 3 cut(s) 14, 1767, 1862
Cfr10I RCCGGY 2 cut(s) 849, 1832
Cfr13I GGNCC 5 cut(s) 356, 1273, 1526, 1915, 2752
CseI GACGC 1 cut(s) 2834
Csp6I GTAC 3 cut(s) 460, 1331, 2017
CspCI CAANNNNNGTGG 2 cut(s) 1579, 1614
CviQI GTAC 3 cut(s) 460, 1331, 2017
DdeI CTNAG 6 cut(s) 156, 167, 675, 2283, 2298, 2544
DpnI GATC 8 cut(s) 270, 348, 570, 988, 1051, 1294, 1884, 2514
DpnII GATC 8 cut(s) 268, 346, 568, 986, 1049, 1292, 1882, 2512
DraI TTTAAA 1 cut(s) 898
DriI GACNNNNNGTC 1 cut(s) 184
EaeI YGGCCR 2 cut(s) 1042, 1349
Eam1104I CTCTTC 1 cut(s) 195
Eam1105I GACNNNNNGTC 1 cut(s) 184
EarI CTCTTC 1 cut(s) 195
EciI GGCGGA 1 cut(s) 1955
Ecl136II GAGCTC 2 cut(s) 2716, 2796
Eco130I CCWWGG 5 cut(s) 369, 481, 706, 990, 2508
Eco24I GRGCYC 2 cut(s) 2718, 2798
Eco31I GGTCTC 2 cut(s) 923, 2279
Eco47I GGWCC 3 cut(s) 356, 1273, 2752
Eco53kI GAGCTC 2 cut(s) 2716, 2796
Eco88I CYCGRG 1 cut(s) 1732
EcoICRI GAGCTC 2 cut(s) 2716, 2796
EcoO109I RGGNCCY 3 cut(s) 356, 1273, 2752
EcoRI GAATTC 1 cut(s) 668
EcoRII CCWGG 6 cut(s) 258, 982, 2082, 2383, 2622, 2694
EcoT14I CCWWGG 5 cut(s) 369, 481, 706, 990, 2508
EcoT38I GRGCYC 2 cut(s) 2718, 2798
ErhI CCWWGG 5 cut(s) 369, 481, 706, 990, 2508
Esp3I CGTCTC 1 cut(s) 153
FalI AAGNNNNNCTT 4 cut(s) 12, 44, 2094, 2126
FaqI GGGAC 6 cut(s) 88, 331, 1379, 2205, 2410, 2738
FauI CCCGC 1 cut(s) 1212
FbaI TGATCA 1 cut(s) 346
FblI GTMKAC 2 cut(s) 59, 1779
Fnu4HI GCNGC 6 cut(s) 161, 546, 799, 1318, 2027, 2819
FokI GGATG 7 cut(s) 321, 394, 1238, 1349, 1487, 1696, 2260
FriOI GRGCYC 2 cut(s) 2718, 2798
Fsp4HI GCNGC 6 cut(s) 161, 546, 799, 1318, 2027, 2819
FspBI CTAG 9 cut(s) 129, 233, 321, 512, 890, 1103, 2313, 2457, 2729
GlaI GCGC 3 cut(s) 13, 1766, 1861
GluI GCNGC 6 cut(s) 161, 546, 799, 1318, 2027, 2819
GsuI CTGGAG 3 cut(s) 1044, 2606, 2624
HaeIII GGCC 7 cut(s) 1044, 1351, 1527, 1916, 2694, 2699, 2871
HapII CCGG 4 cut(s) 850, 1334, 1833, 2743
HgaI GACGC 1 cut(s) 2834
HhaI GCGC 3 cut(s) 14, 1767, 1862
Hin1I GRCGYC 1 cut(s) 2826
Hin6I GCGC 3 cut(s) 12, 1765, 1860
HinP1I GCGC 3 cut(s) 12, 1765, 1860
HincII GTYRAC 5 cut(s) 60, 1780, 2318, 2710, 2790
HindII GTYRAC 5 cut(s) 60, 1780, 2318, 2710, 2790
HpaII CCGG 4 cut(s) 850, 1334, 1833, 2743
HphI GGTGA 2 cut(s) 578, 2462
Hpy166II GTNNAC 7 cut(s) 60, 703, 1780, 2017, 2318, 2710, 2790
Hpy188I TCNGA 7 cut(s) 438, 528, 811, 1470, 2146, 2284, 2299
Hpy8I GTNNAC 7 cut(s) 60, 703, 1780, 2017, 2318, 2710, 2790
Hpy99I CGWCG 8 cut(s) 61, 85, 88, 91, 94, 2472, 2690, 2693
HpyCH4III ACNGT 4 cut(s) 72, 78, 1596, 2783
HpyCH4IV ACGT 2 cut(s) 2008, 2765
HpyF3I CTNAG 6 cut(s) 156, 167, 675, 2283, 2298, 2544
HpySE526I ACGT 2 cut(s) 2008, 2765
Hsp92I GRCGYC 1 cut(s) 2826
HspAI GCGC 3 cut(s) 12, 1765, 1860
Ksp22I TGATCA 1 cut(s) 346
Kzo9I GATC 8 cut(s) 268, 346, 568, 986, 1049, 1292, 1882, 2512
LmnI GCTCC 6 cut(s) 168, 1003, 1063, 1431, 1450, 2801
Lsp1109I GCAGC 5 cut(s) 172, 532, 810, 1304, 2038
LweI GCATC 6 cut(s) 299, 416, 924, 1108, 1489, 2443
MaeI CTAG 9 cut(s) 129, 233, 321, 512, 890, 1103, 2313, 2457, 2729
MaeII ACGT 2 cut(s) 2008, 2765
MaeIII GTNAC 9 cut(s) 72, 1236, 1439, 1670, 2135, 2263, 2476, 2761, 2777
MalI GATC 8 cut(s) 270, 348, 570, 988, 1051, 1294, 1884, 2514
MbiI CCGCTC 1 cut(s) 1090
MboI GATC 8 cut(s) 268, 346, 568, 986, 1049, 1292, 1882, 2512
MboII GAAGA 6 cut(s) 182, 250, 1190, 2262, 2375, 2659
MfeI CAATTG 1 cut(s) 531
MflI RGATCY 3 cut(s) 268, 986, 1292
MhlI GDGCHC 4 cut(s) 1615, 2328, 2718, 2798
MlsI TGGCCA 2 cut(s) 1044, 1351
MluNI TGGCCA 2 cut(s) 1044, 1351
MlyI GAGTC 1 cut(s) 165
MmeI TCCRAC 2 cut(s) 1453, 1668
Mox20I TGGCCA 2 cut(s) 1044, 1351
MroXI GAANNNNTTC 2 cut(s) 1704, 2616
MscI TGGCCA 2 cut(s) 1044, 1351
MslI CAYNNNNRTG 3 cut(s) 307, 2358, 2555
Msp20I TGGCCA 2 cut(s) 1044, 1351
MspA1I CMGCKG 2 cut(s) 2029, 2821
MspCI CTTAAG 1 cut(s) 854
MspI CCGG 4 cut(s) 850, 1334, 1833, 2743
MspR9I CCNGG 6 cut(s) 260, 984, 2084, 2385, 2624, 2696
MunI CAATTG 1 cut(s) 531
Mva1269I GAATGC 2 cut(s) 536, 2394
MvaI CCWGG 6 cut(s) 260, 984, 2084, 2385, 2624, 2696
MvnI CGCG 1 cut(s) 12
NcoI CCATGG 3 cut(s) 481, 706, 2508
NdeII GATC 8 cut(s) 268, 346, 568, 986, 1049, 1292, 1882, 2512
NlaIV GGNNCC 6 cut(s) 270, 358, 402, 988, 1984, 2754
NmeAIII GCCGAG 1 cut(s) 2836
NmuCI GTSAC 4 cut(s) 72, 2135, 2263, 2761
NspI RCATGY 3 cut(s) 888, 1955, 2336
PaeR7I CTCGAG 1 cut(s) 1732
PciI ACATGT 1 cut(s) 2332
PcsI WCGNNNNNNNCGW 3 cut(s) 86, 89, 2771
PctI GAATGC 2 cut(s) 536, 2394
PdmI GAANNNNTTC 2 cut(s) 1704, 2616
PfeI GAWTC 9 cut(s) 578, 1208, 1385, 1849, 1894, 2228, 2294, 2524, 2839
PflFI GACNNNGTC 1 cut(s) 1184
PfoI TCCNGGA 2 cut(s) 2082, 2622
PkrI GCNGC 6 cut(s) 162, 547, 800, 1319, 2028, 2820
PleI GAGTC 1 cut(s) 165
PpsI GAGTC 1 cut(s) 165
PpuMI RGGWCCY 3 cut(s) 356, 1273, 2752
PscI ACATGT 1 cut(s) 2332
Psp124BI GAGCTC 2 cut(s) 2718, 2798
Psp5II RGGWCCY 3 cut(s) 356, 1273, 2752
Psp6I CCWGG 6 cut(s) 258, 982, 2082, 2383, 2622, 2694
PspGI CCWGG 6 cut(s) 258, 982, 2082, 2383, 2622, 2694
PspN4I GGNNCC 6 cut(s) 270, 358, 402, 988, 1984, 2754
PspPI GGNCC 5 cut(s) 356, 1273, 1526, 1915, 2752
PspPPI RGGWCCY 3 cut(s) 356, 1273, 2752
PstI CTGCAG 2 cut(s) 422, 2723
PstNI CAGNNNCTG 2 cut(s) 166, 2029
PsuI RGATCY 3 cut(s) 268, 986, 1292
PsyI GACNNNGTC 1 cut(s) 1184
PvuII CAGCTG 1 cut(s) 2029
RsaI GTAC 3 cut(s) 461, 1332, 2018
RsaNI GTAC 3 cut(s) 460, 1331, 2017
RseI CAYNNNNRTG 3 cut(s) 307, 2358, 2555
SacI GAGCTC 2 cut(s) 2718, 2798
SalI GTCGAC 2 cut(s) 58, 1778
SatI GCNGC 6 cut(s) 161, 546, 799, 1318, 2027, 2819
Sau3AI GATC 8 cut(s) 268, 346, 568, 986, 1049, 1292, 1882, 2512
Sau96I GGNCC 5 cut(s) 356, 1273, 1526, 1915, 2752
SchI GAGTC 1 cut(s) 165
ScrFI CCNGG 6 cut(s) 260, 984, 2084, 2385, 2624, 2696
SduI GDGCHC 4 cut(s) 1615, 2328, 2718, 2798
SfaNI GCATC 6 cut(s) 299, 416, 924, 1108, 1489, 2443
SfcI CTRYAG 3 cut(s) 418, 1282, 2719
Sfr274I CTCGAG 1 cut(s) 1732
SinI GGWCC 3 cut(s) 356, 1273, 2752
SlaI CTCGAG 1 cut(s) 1732
SmiMI CAYNNNNRTG 3 cut(s) 307, 2358, 2555
SmlI CTYRAG 4 cut(s) 854, 1579, 1732, 2747
SmoI CTYRAG 4 cut(s) 854, 1579, 1732, 2747
SpeI ACTAGT 2 cut(s) 320, 2728
SsiI CCGC 8 cut(s) 10, 227, 1075, 1090, 1219, 1966, 2799, 2819
SspI AATATT 2 cut(s) 1878, 2169
SspMI CTAG 9 cut(s) 129, 233, 321, 512, 890, 1103, 2313, 2457, 2729
SstI GAGCTC 2 cut(s) 2718, 2798
StyD4I CCNGG 6 cut(s) 258, 982, 2082, 2383, 2622, 2694
StyI CCWWGG 5 cut(s) 369, 481, 706, 990, 2508
TaaI ACNGT 4 cut(s) 72, 78, 1596, 2783
TaiI ACGT 2 cut(s) 2011, 2768
TaqI TCGA 4 cut(s) 59, 837, 1733, 1779
TatI WGTACW 2 cut(s) 459, 2016
TauI GCSGC 1 cut(s) 2821
TfiI GAWTC 9 cut(s) 578, 1208, 1385, 1849, 1894, 2228, 2294, 2524, 2839
TscAI CASTG 5 cut(s) 309, 928, 1601, 2452, 2865
TseFI GTSAC 4 cut(s) 72, 2135, 2263, 2761
TseI GCWGC 5 cut(s) 160, 545, 798, 1317, 2026
Tsp45I GTSAC 4 cut(s) 72, 2135, 2263, 2761
TspDTI ATGAA 9 cut(s) 378, 876, 1041, 1377, 2007, 2196, 2262, 2376, 2539
TspGWI ACGGA 1 cut(s) 2266
TspRI CASTG 5 cut(s) 309, 928, 1601, 2452, 2865
Tth111I GACNNNGTC 1 cut(s) 1184
Vha464I CTTAAG 1 cut(s) 854
VpaK11BI GGWCC 3 cut(s) 356, 1273, 2752
XapI RAATTY 6 cut(s) 668, 789, 893, 1703, 2118, 2570
XbaI TCTAGA 1 cut(s) 889
XceI RCATGY 3 cut(s) 888, 1955, 2336
XcmI CCANNNNNNNNNTGG 1 cut(s) 148
XhoI CTCGAG 1 cut(s) 1732
XmiI GTMKAC 2 cut(s) 59, 1779
XmnI GAANNNNTTC 2 cut(s) 1704, 2616
XspI CTAG 9 cut(s) 129, 233, 321, 512, 890, 1103, 2313, 2457, 2729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.