Rh2CG311100

DNA excision repair protein ERCC-6-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0013205
Physical Location & Seq
Forward (+)
39291126 .. 39297039
5914 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG311100.1

Sequence Viewer

Length: 2637 bp
ATGTCTCTCCGCGCCTTCAAAGAGAGCCTTAAACCCTGCCAAACCCAAAACTCCTCGTCGACCCAAACACAGGCGTCGTCGTCGTCGTCAATTCTGTCCCAAATCCCAGAACCCACCATCCCTAGAAAGCCCCCCAAATCGTCTCTGGCTCAGCAGCTCCTGAGGCTCCAAGACCCTCTGTCTCTTCAACCACCTCCACCTCCACCACCGCCACCGCCCCTAGAGGAGACCCAGGTGGAGGAGGACGAAGACGACCCAGAACCACAGGGATGCTCTGGGACTAGTAAAGTTAGTAGCTTTCAGTTTGATCACACAGGACCCTATGAACCCTTGGTCTTATCCCATTTTCCTCTCATTCAGGTTCCTGCATCCATTAACTGCAGATTGCTTCCACATCAGAGGGAGGGAGTAAAGTTTCTATACAATATATACAAGAACAACCATGGAGGGGTTCTTGGAGATGACATGGGACTAGGCAAAACCATTCAAACAATTGCATTCTTGGCTGCTGTGTTTGGGAAGGATGGTGATTGCATTGATTCCACAATTCCGAAGAGAAGTGAAATAGCAGAACGAAACCCTGTTCTCATAGTTTGCCCCAGTTCAGTTATCCACAACTGGGAGAGTGAATTCTCTAAGTGGTCTAACTTTAGTGTTGCTGTTTACCATGGTGCAAATCGGGATTTGATTTATGATAAATTGGAAGCTCATGGAGTTGAGATACTTATCACGAGCTTTGACACATACAGAATTTGTGGCAGCCAGTTATCAGATGTTAATTGGGAGATTGTTGTTGTCGATGAGGCACACCGGCTTAAGAATGAAAAATCAAAGCTCTACATAGCATGTCTAGAATTCAAAACCTTAAAACGAATTGGTCTCACTGGAACTATGATGCAGAACAAAATCATGGAATTGTTTAACCTGTTTGACTGGGTTGCACCTGGATCCTTGGGAACACGGGAGCATTTTCGGGAGTTTTATGATGAACCTCTCAAACATGGCCAAAGATCAACTGCTCCAGAAAGATTTGTCCGCATTGCTGATGAGCGAAAACAGCATCTAGTAGCTGTTCTTAATAAATACATGTTGAGAAGGACAAAAGAGGAAACCATTGGTCATCTTATGATGGGGAAGGAAGACAATGTCATATTTTGTTCAATGAGCGAATTGCAAAAGCGGGTGTATAGGCGAATGTTACAGCTACCAGACATCCAATGCCTGATAAATAAGGACCTTCCCTGTAGTTGTGGATCTCCTCTTACCCAAGTTGAGTGCTGCAAAAGGATTGTACCAGATGGAAAAATCTGGCCATACCTTCACAGGGATAACCCTGATGGTTGTGATTCATGCCCCTTTTGTATTGTCCTTCCTTGCCTTGTCAAGCTCCAACAGATAAGTAACCACTTGGAGCTTATTAAGCCTAATCCGAAGGATGACCCAGAAAAGCAAAAGAAAGATGCAGAGTTTGCCTCTGCTGTGTTTGGCCCAGATATTGATTTGGTGGGAGGCAACACTCAAAATGAGAGTTTTATGGGCTTAAGTGATGTTAAACAGTGTGGGAAAATGCGAGCACTTGAAAAGTTTATGTTCTCATGGATTGCACAGGGTGATAAAGTCCTTCTGTTCAGTTACTCTGTCAGGATGCTGGACATATTGGAAAAATTTCTTATACGCAAAGGTTATTGCTTCTCAAGACTTGATGGTTCCACACCAACCAACTTGCGCCAGGCTCTTGTTGACAACTTTAATTCAAGTCCAAGCAAACAGGTGTTCCTTATATCAACTCGAGCCGGTGGGCTTGGACTGAATCTTGTCAGTGCAAATCGTGTTGTAATATTTGATCCAAGCTGGAATCCTGCCCAAGACTTACAGGCCCAAGACAGGTCATTTCGGTTTGGGCAGAAACGGCATGTTGTGGTTTTCCGCCTTCTTTCTGCTGGTTCCCTTGATGAACTTGTTTATTCACGTCAGGTGTACAAACAGCAGCTATCAAACATTGCTGTTTCTGGAAAAATGGAGAGACGATATTTTGAAGGTGTCCAGGATTGCAAAGACTTTCAAGGGGAACTTTTTGGAATTTGCAATTTATTTCGTGATCTTTCAGATAAGCTCTTTACCAGCGAGATATTTGAGTTGCATGAAAAGCAGGGACAAAGAGAAGGGGATTGTCATGGTAGGGAACAAGAATCAACCAAAATTACTTGCGTAAAAGAAGTGGGTGTGACATCTTCATCCATGTCTGAGACCACGGATTCTGAGAAGGCTTTAGCTAGTGAACCTGTGCTTAAAGATGTGGGTATTGTCTATGCACATCGTAATGAAGACATTGTGAATAATAGACGTGGATTGCAAGGGACAATGGAAAGAGTCATTCCTTGTAATGACAGCCTAAAACAGCCCCCCACTGGTGTTGCTAGGAGAAAGCGACGAGAGGGTACTGGTGGAAAGGAGAATGTATCTGTATCCATGGATCGCAAAAGGATTCAATTTAGTCTCCTTGCTAAGTTCATGGGTATGGGAGAGATTGAATTTAGCAAGTGGGTAGTATCTGCTCCTCCTCTAGAGAGGGAAAAGGTTATCCAGGACTACAAGGAGAGAAATAAGAAGAAACTCAACAGCGTTGATTTGTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helicase_C SNF2_N

Protein Analysis

878

Amino Acids

99.25

Weight (kDa)

7.89

Isoelectric Point (pI)

45.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 127 - 298 2.2e-08 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 132 - 406 3e-65 SNF2-related domain
DEAD PF00270 151 - 301 5.9e-06 DEAD/DEAH box helicase
Helicase_C PF00271 522 - 634 2.3e-23 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013205)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03750
fragaria_vesca FvH4_6g26480 FvH4_6g26480 FvH4_6g26480 FvH4_6g26480
malus_domestica MD17G1236900.v1.1
prunus_persica Prupe.3G139100_v2.0.a1
pyrus_communis pycom17g24160
rosa_chinensis RchiOBHm_Chr2g0127761
rosa_laevigata RLG00000018935
rosa_multiflora Rmu_sc0001829.1_g000008 Rmu_sc0004240.1_g000009
rosa_roxburghii Rroxscaffold_2G00116780
rosa_rugosa Rorug02G0271200
rosa_samantha Rh2AG323300 Rh2BG332500 Rh2CG311100
rosa_wichuraiana Rw2G026240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2635
AccI GTMKAC 1 cut(s) 59
AccII CGCG 1 cut(s) 12
AciI CCGC 6 cut(s) 10, 209, 215, 1036, 1180, 1927
AclWI GGATC 5 cut(s) 942, 955, 1261, 1838, 2481
AcoI YGGCCR 2 cut(s) 1003, 1310
AcsI RAATTY 6 cut(s) 629, 750, 854, 1664, 2079, 2531
AcyI GRCGYC 1 cut(s) 74
AdeI CACNNNGTG 1 cut(s) 1610
AfaI GTAC 3 cut(s) 1293, 1979, 2440
AfiI CCNNNNNNNGG 7 cut(s) 70, 238, 448, 619, 1324, 1884, 2408
AflII CTTAAG 2 cut(s) 815, 1540
AflIII ACRYGT 1 cut(s) 1086
AhdI GACNNNNNGTC 1 cut(s) 178
AhlI ACTAGT 1 cut(s) 281
AjiI CACGTC 2 cut(s) 1970, 2345
AjnI CCWGG 5 cut(s) 231, 943, 1728, 2043, 2583
AloI GAACNNNNNNTCC 2 cut(s) 102, 134
Alw21I GWGCWC 1 cut(s) 1576
Alw26I GTCTC 8 cut(s) 9, 147, 186, 221, 884, 2017, 2240, 2501
AlwI GGATC 5 cut(s) 942, 955, 1261, 1838, 2481
AlwNI CAGNNNCTG 1 cut(s) 160
Ama87I CYCGRG 1 cut(s) 1788
AoxI GGCC 4 cut(s) 1003, 1310, 1486, 1875
ApeKI GCWGC 5 cut(s) 154, 506, 759, 1278, 1987
ApoI RAATTY 6 cut(s) 629, 750, 854, 1664, 2079, 2531
Asp700I GAANNNNTTC 1 cut(s) 1665
AspLEI GCGC 2 cut(s) 14, 1728
AspS9I GGNCC 4 cut(s) 317, 1234, 1487, 1876
AsuHPI GGTGA 2 cut(s) 539, 1622
AvaI CYCGRG 1 cut(s) 1788
AvaII GGWCC 2 cut(s) 317, 1234
AxyI CCTNAGG 1 cut(s) 161
BalI TGGCCA 2 cut(s) 1005, 1312
BamHI GGATCC 1 cut(s) 947
BauI CACGAG 1 cut(s) 730
BbsI GAAGAC 3 cut(s) 255, 1146, 2331
Bbv12I GWGCWC 1 cut(s) 1576
BbvI GCAGC 5 cut(s) 166, 493, 771, 1265, 1999
BccI CCATC 6 cut(s) 125, 518, 1123, 1292, 1331, 1697
BceAI ACGGC 1 cut(s) 1925
BciT130I CCWGG 5 cut(s) 233, 945, 1730, 2045, 2585
BciVI GTATCC 1 cut(s) 2476
BclI TGATCA 1 cut(s) 307
BcoDI GTCTC 8 cut(s) 9, 147, 186, 221, 884, 2017, 2240, 2501
BcuI ACTAGT 1 cut(s) 281
BfaI CTAG 9 cut(s) 123, 221, 282, 473, 851, 1064, 2274, 2418, 2564
BfmI CTRYAG 2 cut(s) 379, 1243
BfrI CTTAAG 2 cut(s) 815, 1540
BfuI GTATCC 1 cut(s) 2476
BisI GCNGC 5 cut(s) 155, 507, 760, 1279, 1988
BlpI GCTNAGC 1 cut(s) 150
BlsI GCNGC 5 cut(s) 156, 508, 761, 1280, 1989
Bme1390I CCNGG 5 cut(s) 233, 945, 1730, 2045, 2585
Bme18I GGWCC 2 cut(s) 317, 1234
BmeRI GACNNNNNGTC 1 cut(s) 178
BmeT110I CYCGRG 1 cut(s) 1788
BmgBI CACGTC 2 cut(s) 1970, 2345
BmgT120I GGNCC 4 cut(s) 317, 1234, 1487, 1876
BmiI GGNNCC 6 cut(s) 167, 319, 363, 949, 1708, 1945
BmrFI CCNGG 5 cut(s) 233, 945, 1730, 2045, 2585
BmrI ACTGGG 3 cut(s) 594, 628, 943
BmsI GCATC 6 cut(s) 260, 377, 885, 1069, 1450, 1635
BmuI ACTGGG 3 cut(s) 594, 628, 943
BpiI GAAGAC 3 cut(s) 255, 1146, 2331
BplI GAGNNNNNCTC 2 cut(s) 1457, 1489
BpmI CTGGAG 1 cut(s) 1005
Bpu1102I GCTNAGC 1 cut(s) 150
BpuEI CTTGAG 1 cut(s) 1678
BsaBI GATNNNNATC 1 cut(s) 725
BsaHI GRCGYC 1 cut(s) 74
BsaI GGTCTC 3 cut(s) 221, 884, 2240
BsaJI CCNNGG 7 cut(s) 231, 330, 442, 667, 951, 2250, 2469
Bsc4I CCNNNNNNNGG 7 cut(s) 70, 238, 448, 619, 1324, 1884, 2408
Bse118I RCCGGY 2 cut(s) 810, 1793
Bse1I ACTGG 7 cut(s) 600, 623, 763, 889, 938, 2413, 2446
Bse21I CCTNAGG 1 cut(s) 161
Bse3DI GCAATG 2 cut(s) 1038, 1998
Bse8I GATNNNNATC 1 cut(s) 725
BseBI CCWGG 5 cut(s) 233, 945, 1730, 2045, 2585
BseDI CCNNGG 7 cut(s) 231, 330, 442, 667, 951, 2250, 2469
BseGI GGATG 8 cut(s) 117, 275, 368, 529, 1212, 1441, 1650, 2234
BseJI GATNNNNATC 1 cut(s) 725
BseLI CCNNNNNNNGG 7 cut(s) 70, 238, 448, 619, 1324, 1884, 2408
BseMI GCAATG 2 cut(s) 1038, 1998
BseMII CTCAG 4 cut(s) 152, 164, 2235, 2250
BseNI ACTGG 7 cut(s) 600, 623, 763, 889, 938, 2413, 2446
BseRI GAGGAG 6 cut(s) 43, 239, 254, 1248, 2547, 2550
BseXI GCAGC 5 cut(s) 166, 493, 771, 1265, 1999
Bsh1236I CGCG 1 cut(s) 12
BshFI GGCC 4 cut(s) 1005, 1312, 1488, 1877
BsiHKAI GWGCWC 1 cut(s) 1576
BsiHKCI CYCGRG 1 cut(s) 1788
BsiSI CCGG 2 cut(s) 811, 1794
BslFI GGGAC 5 cut(s) 82, 292, 483, 2166, 2371
BslI CCNNNNNNNGG 7 cut(s) 70, 238, 448, 619, 1324, 1884, 2408
BsmAI GTCTC 8 cut(s) 9, 147, 186, 221, 884, 2017, 2240, 2501
BsmBI CGTCTC 2 cut(s) 147, 2017
BsmFI GGGAC 5 cut(s) 82, 292, 483, 2166, 2371
BsmI GAATGC 1 cut(s) 497
BsnI GGCC 4 cut(s) 1005, 1312, 1488, 1877
Bso31I GGTCTC 3 cut(s) 221, 884, 2240
BsoBI CYCGRG 1 cut(s) 1788
Bsp1286I GDGCHC 1 cut(s) 1576
Bsp1407I TGTACA 1 cut(s) 1977
Bsp143I GATC 7 cut(s) 307, 947, 1010, 1253, 1843, 2098, 2473
Bsp1720I GCTNAGC 1 cut(s) 150
Bsp19I CCATGG 3 cut(s) 442, 667, 2469
BspACI CCGC 6 cut(s) 10, 209, 215, 1036, 1180, 1927
BspANI GGCC 4 cut(s) 1005, 1312, 1488, 1877
BspCNI CTCAG 4 cut(s) 153, 163, 2236, 2251
BspFNI CGCG 1 cut(s) 12
BspLI GGNNCC 6 cut(s) 167, 319, 363, 949, 1708, 1945
BspMAI CTGCAG 1 cut(s) 383
BspPI GGATC 5 cut(s) 942, 955, 1261, 1838, 2481
BspTI CTTAAG 2 cut(s) 815, 1540
BspTNI GGTCTC 3 cut(s) 221, 884, 2240
BsrDI GCAATG 2 cut(s) 1038, 1998
BsrFI RCCGGY 2 cut(s) 810, 1793
BsrGI TGTACA 1 cut(s) 1977
BsrI ACTGG 7 cut(s) 600, 623, 763, 889, 938, 2413, 2446
BssAI RCCGGY 2 cut(s) 810, 1793
BssECI CCNNGG 7 cut(s) 231, 330, 442, 667, 951, 2250, 2469
BssMI GATC 7 cut(s) 307, 947, 1010, 1253, 1843, 2098, 2473
BssNI GRCGYC 1 cut(s) 74
BssSI CACGAG 1 cut(s) 730
BssT1I CCWWGG 5 cut(s) 330, 442, 667, 951, 2469
Bst2BI CACGAG 1 cut(s) 730
Bst2UI CCWGG 5 cut(s) 233, 945, 1730, 2045, 2585
Bst4CI ACNGT 1 cut(s) 1557
Bst6I CTCTTC 2 cut(s) 189, 548
BstACI GRCGYC 1 cut(s) 74
BstAFI CTTAAG 2 cut(s) 815, 1540
BstAPI GCANNNNNTGC 1 cut(s) 1469
BstAUI TGTACA 1 cut(s) 1977
BstC8I GCNNGC 1 cut(s) 1572
BstDEI CTNAG 6 cut(s) 150, 161, 636, 2244, 2259, 2505
BstDSI CCRYGG 4 cut(s) 442, 667, 2250, 2469
BstF5I GGATG 8 cut(s) 117, 275, 368, 529, 1212, 1441, 1650, 2234
BstFNI CGCG 1 cut(s) 12
BstHHI GCGC 2 cut(s) 14, 1728
BstKTI GATC 7 cut(s) 310, 950, 1013, 1256, 1846, 2101, 2476
BstMAI GTCTC 8 cut(s) 9, 147, 186, 221, 884, 2017, 2240, 2501
BstMBI GATC 7 cut(s) 307, 947, 1010, 1253, 1843, 2098, 2473
BstMWI GCNNNNNNNGC 7 cut(s) 163, 503, 1057, 1420, 1469, 1909, 2146
BstNI CCWGG 5 cut(s) 233, 945, 1730, 2045, 2585
BstNSI RCATGY 3 cut(s) 849, 1090, 1916
BstSCI CCNGG 5 cut(s) 231, 943, 1728, 2043, 2583
BstSFI CTRYAG 2 cut(s) 379, 1243
BstUI CGCG 1 cut(s) 12
BstV1I GCAGC 5 cut(s) 166, 493, 771, 1265, 1999
BstV2I GAAGAC 3 cut(s) 255, 1146, 2331
BstX2I RGATCY 2 cut(s) 947, 1253
BstYI RGATCY 2 cut(s) 947, 1253
Bsu36I CCTNAGG 1 cut(s) 161
BsuI GTATCC 1 cut(s) 2476
BsuRI GGCC 4 cut(s) 1005, 1312, 1488, 1877
BtgI CCRYGG 4 cut(s) 442, 667, 2250, 2469
BtrI CACGTC 2 cut(s) 1970, 2345
BtsCI GGATG 8 cut(s) 117, 275, 368, 529, 1212, 1441, 1650, 2234
BtsIMutI CAGTG 4 cut(s) 882, 1562, 1825, 2406
Cac8I GCNNGC 1 cut(s) 1572
CaiI CAGNNNCTG 1 cut(s) 160
CfoI GCGC 2 cut(s) 14, 1728
Cfr10I RCCGGY 2 cut(s) 810, 1793
Cfr13I GGNCC 4 cut(s) 317, 1234, 1487, 1876
CseI GACGC 1 cut(s) 63
Csp6I GTAC 3 cut(s) 1292, 1978, 2439
CspCI CAANNNNNGTGG 2 cut(s) 2395, 2430
CviQI GTAC 3 cut(s) 1292, 1978, 2439
DdeI CTNAG 6 cut(s) 150, 161, 636, 2244, 2259, 2505
DpnI GATC 7 cut(s) 309, 949, 1012, 1255, 1845, 2100, 2475
DpnII GATC 7 cut(s) 307, 947, 1010, 1253, 1843, 2098, 2473
DraIII CACNNNGTG 1 cut(s) 1610
DriI GACNNNNNGTC 1 cut(s) 178
EaeI YGGCCR 2 cut(s) 1003, 1310
Eam1104I CTCTTC 2 cut(s) 189, 548
Eam1105I GACNNNNNGTC 1 cut(s) 178
EarI CTCTTC 2 cut(s) 189, 548
EciI GGCGGA 1 cut(s) 1916
Eco130I CCWWGG 5 cut(s) 330, 442, 667, 951, 2469
Eco31I GGTCTC 3 cut(s) 221, 884, 2240
Eco47I GGWCC 2 cut(s) 317, 1234
Eco81I CCTNAGG 1 cut(s) 161
Eco88I CYCGRG 1 cut(s) 1788
EcoO109I RGGNCCY 2 cut(s) 317, 1234
EcoRI GAATTC 2 cut(s) 629, 854
EcoRII CCWGG 5 cut(s) 231, 943, 1728, 2043, 2583
EcoT14I CCWWGG 5 cut(s) 330, 442, 667, 951, 2469
ErhI CCWWGG 5 cut(s) 330, 442, 667, 951, 2469
Esp3I CGTCTC 2 cut(s) 147, 2017
FalI AAGNNNNNCTT 4 cut(s) 12, 44, 2055, 2087
FaqI GGGAC 5 cut(s) 82, 292, 483, 2166, 2371
FauI CCCGC 1 cut(s) 1173
FbaI TGATCA 1 cut(s) 307
FblI GTMKAC 1 cut(s) 59
Fnu4HI GCNGC 5 cut(s) 155, 507, 760, 1279, 1988
FokI GGATG 8 cut(s) 104, 282, 355, 536, 1199, 1448, 1657, 2221
Fsp4HI GCNGC 5 cut(s) 155, 507, 760, 1279, 1988
FspBI CTAG 9 cut(s) 123, 221, 282, 473, 851, 1064, 2274, 2418, 2564
GlaI GCGC 2 cut(s) 13, 1727
GluI GCNGC 5 cut(s) 155, 507, 760, 1279, 1988
GsuI CTGGAG 1 cut(s) 1005
HaeIII GGCC 4 cut(s) 1005, 1312, 1488, 1877
HapII CCGG 2 cut(s) 811, 1794
HgaI GACGC 1 cut(s) 63
HhaI GCGC 2 cut(s) 14, 1728
Hin1I GRCGYC 1 cut(s) 74
Hin6I GCGC 2 cut(s) 12, 1726
HinP1I GCGC 2 cut(s) 12, 1726
HincII GTYRAC 2 cut(s) 60, 1741
HindII GTYRAC 2 cut(s) 60, 1741
HinfI GANTC 8 cut(s) 539, 1346, 1810, 1855, 2189, 2255, 2370, 2485
HpaII CCGG 2 cut(s) 811, 1794
HphI GGTGA 2 cut(s) 539, 1622
Hpy166II GTNNAC 5 cut(s) 60, 664, 1741, 1978, 2279
Hpy188I TCNGA 7 cut(s) 399, 552, 772, 1431, 2107, 2245, 2260
Hpy8I GTNNAC 5 cut(s) 60, 664, 1741, 1978, 2279
Hpy99I CGWCG 6 cut(s) 61, 79, 82, 85, 88, 2433
HpyCH4III ACNGT 1 cut(s) 1557
HpyCH4IV ACGT 2 cut(s) 1969, 2344
HpyF10VI GCNNNNNNNGC 7 cut(s) 163, 503, 1057, 1420, 1469, 1909, 2146
HpyF3I CTNAG 6 cut(s) 150, 161, 636, 2244, 2259, 2505
HpySE526I ACGT 2 cut(s) 1969, 2344
Hsp92I GRCGYC 1 cut(s) 74
HspAI GCGC 2 cut(s) 12, 1726
Ksp22I TGATCA 1 cut(s) 307
Kzo9I GATC 7 cut(s) 307, 947, 1010, 1253, 1843, 2098, 2473
LmnI GCTCC 7 cut(s) 162, 171, 964, 1024, 1392, 1411, 2560
Lsp1109I GCAGC 5 cut(s) 166, 493, 771, 1265, 1999
LweI GCATC 6 cut(s) 260, 377, 885, 1069, 1450, 1635
MaeI CTAG 9 cut(s) 123, 221, 282, 473, 851, 1064, 2274, 2418, 2564
MaeII ACGT 2 cut(s) 1969, 2344
MaeIII GTNAC 4 cut(s) 1197, 1400, 1631, 2224
MalI GATC 7 cut(s) 309, 949, 1012, 1255, 1845, 2100, 2475
MboI GATC 7 cut(s) 307, 947, 1010, 1253, 1843, 2098, 2473
MboII GAAGA 7 cut(s) 176, 260, 565, 1151, 2223, 2336, 2620
MfeI CAATTG 1 cut(s) 492
MflI RGATCY 2 cut(s) 947, 1253
MhlI GDGCHC 1 cut(s) 1576
MlsI TGGCCA 2 cut(s) 1005, 1312
MluNI TGGCCA 2 cut(s) 1005, 1312
MlyI GAGTC 1 cut(s) 2379
MmeI TCCRAC 1 cut(s) 1414
Mox20I TGGCCA 2 cut(s) 1005, 1312
MroXI GAANNNNTTC 1 cut(s) 1665
MscI TGGCCA 2 cut(s) 1005, 1312
MslI CAYNNNNRTG 3 cut(s) 268, 2319, 2516
Msp20I TGGCCA 2 cut(s) 1005, 1312
MspCI CTTAAG 2 cut(s) 815, 1540
MspI CCGG 2 cut(s) 811, 1794
MspR9I CCNGG 5 cut(s) 233, 945, 1730, 2045, 2585
MunI CAATTG 1 cut(s) 492
Mva1269I GAATGC 1 cut(s) 497
MvaI CCWGG 5 cut(s) 233, 945, 1730, 2045, 2585
MvnI CGCG 1 cut(s) 12
MwoI GCNNNNNNNGC 7 cut(s) 163, 503, 1057, 1420, 1469, 1909, 2146
NcoI CCATGG 3 cut(s) 442, 667, 2469
NdeII GATC 7 cut(s) 307, 947, 1010, 1253, 1843, 2098, 2473
NlaIV GGNNCC 6 cut(s) 167, 319, 363, 949, 1708, 1945
NmuCI GTSAC 1 cut(s) 2224
NspI RCATGY 3 cut(s) 849, 1090, 1916
PaeR7I CTCGAG 1 cut(s) 1788
PciI ACATGT 1 cut(s) 1086
PcsI WCGNNNNNNNCGW 1 cut(s) 83
PctI GAATGC 1 cut(s) 497
PdmI GAANNNNTTC 1 cut(s) 1665
PfeI GAWTC 7 cut(s) 539, 1346, 1810, 1855, 2189, 2255, 2485
PflFI GACNNNGTC 1 cut(s) 1145
PfoI TCCNGGA 2 cut(s) 2043, 2583
PkrI GCNGC 5 cut(s) 156, 508, 761, 1280, 1989
PleI GAGTC 1 cut(s) 2378
PpsI GAGTC 1 cut(s) 2378
PpuMI RGGWCCY 2 cut(s) 317, 1234
PscI ACATGT 1 cut(s) 1086
PsiI TTATAA 1 cut(s) 2635
Psp5II RGGWCCY 2 cut(s) 317, 1234
Psp6I CCWGG 5 cut(s) 231, 943, 1728, 2043, 2583
PspGI CCWGG 5 cut(s) 231, 943, 1728, 2043, 2583
PspN4I GGNNCC 6 cut(s) 167, 319, 363, 949, 1708, 1945
PspPI GGNCC 4 cut(s) 317, 1234, 1487, 1876
PspPPI RGGWCCY 2 cut(s) 317, 1234
PspXI VCTCGAGB 1 cut(s) 1788
PstI CTGCAG 1 cut(s) 383
PstNI CAGNNNCTG 1 cut(s) 160
PsuI RGATCY 2 cut(s) 947, 1253
PsyI GACNNNGTC 1 cut(s) 1145
RsaI GTAC 3 cut(s) 1293, 1979, 2440
RsaNI GTAC 3 cut(s) 1292, 1978, 2439
RseI CAYNNNNRTG 3 cut(s) 268, 2319, 2516
SalI GTCGAC 1 cut(s) 58
SatI GCNGC 5 cut(s) 155, 507, 760, 1279, 1988
Sau3AI GATC 7 cut(s) 307, 947, 1010, 1253, 1843, 2098, 2473
Sau96I GGNCC 4 cut(s) 317, 1234, 1487, 1876
SchI GAGTC 1 cut(s) 2379
ScrFI CCNGG 5 cut(s) 233, 945, 1730, 2045, 2585
SduI GDGCHC 1 cut(s) 1576
SfaNI GCATC 6 cut(s) 260, 377, 885, 1069, 1450, 1635
SfcI CTRYAG 2 cut(s) 379, 1243
Sfr274I CTCGAG 1 cut(s) 1788
SinI GGWCC 2 cut(s) 317, 1234
SlaI CTCGAG 1 cut(s) 1788
SmiMI CAYNNNNRTG 3 cut(s) 268, 2319, 2516
SmlI CTYRAG 4 cut(s) 815, 1540, 1693, 1788
SmoI CTYRAG 4 cut(s) 815, 1540, 1693, 1788
SpeI ACTAGT 1 cut(s) 281
SsiI CCGC 6 cut(s) 10, 209, 215, 1036, 1180, 1927
SspI AATATT 1 cut(s) 1839
SspMI CTAG 9 cut(s) 123, 221, 282, 473, 851, 1064, 2274, 2418, 2564
StyD4I CCNGG 5 cut(s) 231, 943, 1728, 2043, 2583
StyI CCWWGG 5 cut(s) 330, 442, 667, 951, 2469
TaaI ACNGT 1 cut(s) 1557
TaiI ACGT 2 cut(s) 1972, 2347
TaqI TCGA 3 cut(s) 59, 798, 1789
TatI WGTACW 1 cut(s) 1977
TfiI GAWTC 7 cut(s) 539, 1346, 1810, 1855, 2189, 2255, 2485
TscAI CASTG 4 cut(s) 889, 1562, 1825, 2413
TseFI GTSAC 1 cut(s) 2224
TseI GCWGC 5 cut(s) 154, 506, 759, 1278, 1987
Tsp45I GTSAC 1 cut(s) 2224
TspDTI ATGAA 9 cut(s) 339, 837, 1002, 1338, 1968, 2157, 2223, 2337, 2500
TspGWI ACGGA 1 cut(s) 2267
TspRI CASTG 4 cut(s) 889, 1562, 1825, 2413
Tth111I GACNNNGTC 1 cut(s) 1145
Vha464I CTTAAG 2 cut(s) 815, 1540
VpaK11BI GGWCC 2 cut(s) 317, 1234
XapI RAATTY 6 cut(s) 629, 750, 854, 1664, 2079, 2531
XbaI TCTAGA 2 cut(s) 850, 2563
XceI RCATGY 3 cut(s) 849, 1090, 1916
XcmI CCANNNNNNNNNTGG 1 cut(s) 142
XhoI CTCGAG 1 cut(s) 1788
XmiI GTMKAC 1 cut(s) 59
XmnI GAANNNNTTC 1 cut(s) 1665
XspI CTAG 9 cut(s) 123, 221, 282, 473, 851, 1064, 2274, 2418, 2564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.