Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0009537

Physical Location & Seq

Genomic Coordinates Forward (+)

28910006 .. 28914603

Gene Structure 4598 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1239800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1332 bp

ATGATCTTGGGTTCCTCTGCAGAGAAGAAGAAAATGGTAGTGGCAGGAAATGGCCTGTTCTACCCGATTATTGGATTCGCATCGTGTGTGGCCTTCATCTACATGTCTTTCGGTAACTTGGGGTCGAATTTTGGCAACCAAACGAAGTTGAGTTTTGTGGGGAGGAATGGGACTCAGTTCATGTTGGATGGCAAGGCATTTTACATTAATGGGTGGAATTCTTACTGGCTGATGGATAATAGTGTGGATGAGCATAGAAAGCCGAGGGTTCGGCAGATACTGCAAGCCGGTGCGAAAATGGGCCTCACCGTCTGCCGGACTTGGGCGTTTAATGACGGCGACTACAATGCCCTCCAGATTTCCCCTGGTCACTTCAATGAGCAAGTGTTCAAGGCTTTGGATCATGTCATTGTAGAAGCTAGACAACAGGGCATCAGGTTGCTTCTTTGCTTAGTTAATAACTTGCAAGCATATGGCGGGAAGACTCAGTATGTAAAGTGGGCATGGGAAGAAGGTGTTGGTTTGAGCGCTTCCAATGACTCATTTTTCTTCGATCCATCTATTCGCATTTATTTCAAGAACTACGTCAAGACTCTCCTAACAAGGAAGAATACTCTCAATGGAATTCAGTATAGAAATGATCCTACCATTTTCGCGTGGGAATTAATAAACGAACCTCGTTGCATAACTGATGCTTCAGGTGACACTCTCCAAGATTGGATAGAAGAAATGTCAGCTTTTGTGAAAGCAATTGACAAGAACCATCTACTGACTGTGGGCCTTGAAGGGTTTTATGGTCCTAAAAGCCCTAAAAGGTTAACTGTGAATCCAGAAATGTGGGCATCCAGACTCGGCTCAGATTTTATTCGCAACTCCAAGATCCCCAATATTGATTTTGCCTCTGTTCATATCTACCCTGACCATTGGTTCCATGATGAAGAATTTCACAATAACCTTAAATTTGTCTACAAGTGGATGCGTTCTCACATTGAAGACGGAGACAATGAACTGAACAAACCTGTCATGTTCACCGAATATGGATTGTCAAACCAGAACAAGGACTTTGAACCATCTCAGCGTGTTAGGTTTTACAAAACTATTTTAGACGTGATATACAAATCTGCCAAGAAAAACCAGTCTGGAGCAGGCGCTTTAGCCTGGCAGTTTTTTGTTGGAGGAATGGACGAGTATAATGATGAGTTTGGGATAATTCCGTGGGAAAGTCCGTCTGAGTACAAGATGATGATAGAACAATCGTGCAGGTTGGCTAGACTCAATGGACCAACTCAACACCAGCGAAATTTGAAAGACTTGTGTTCGCAAAGACAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004553 GO:0004567 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010412 GO:0015923 GO:0016052 GO:0016787 GO:0016798 GO:0016985 GO:0016998 GO:0043170 GO:0044036 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0046355 GO:0071554 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00051 map00051

Pfam Domains

Protein Families

Cellulase

Protein Analysis

444

Amino Acids

50.92

Weight (kDa)

8.07

Isoelectric Point (pI)

39.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GH5_mannosidase	PF26410	51 - 389	7.3e-52	GH family 5 beta-mannosidase-like
Cellulase	PF00150	121 - 361	1.3e-11	Cellulase (glycosyl hydrolase family 5)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009537)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20680 AT4G28320
fragaria_vesca	FvH4_6g26240 FvH4_6g26240 FvH4_6g26240
malus_domestica	MD09G1246900.v1.1 MD17G1239800.v1.1
prunus_persica	Prupe.3G132400_v2.0.a1 Prupe.3G132400_v2.0.a1 Prupe.3G132400_v2.0.a1 Prupe.3G132400_v2.0.a1
pyrus_communis	pycom09g16420 pycom17g24310
rosa_chinensis	RchiOBHm_Chr2g0126631
rosa_laevigata	RLG00000018915
rosa_multiflora	Rmu_sc0002369.1_g000020
rosa_roxburghii	Rroxscaffold_2G00117060
rosa_rugosa	Rorug02G0264100
rosa_samantha	Rh2AG321100 Rh2BG329400 Rh2CG309100
rosa_wichuraiana	Rw2G026020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1251
AccI	GTMKAC	1 cut(s)	966
AccII	CGCG	1 cut(s)	656
AciI	CCGC	1 cut(s)	477
AclWI	GGATC	4 cut(s)	408, 548, 635, 874
AcsI	RAATTY	6 cut(s)	127, 217, 624, 941, 959, 1300
AcuI	CTGAAG	1 cut(s)	681
AfaI	GTAC	1 cut(s)	1235
AfeI	AGCGCT	1 cut(s)	529
AfiI	CCNNNNNNNGG	4 cut(s)	71, 315, 322, 1057
AflIII	ACRYGT	1 cut(s)	102
AgsI	TTSAA	7 cut(s)	376, 391, 577, 785, 992, 1067, 1306
AjiI	CACGTC	1 cut(s)	1108
AjnI	CCWGG	2 cut(s)	364, 1157
AjuI	GAANNNNNNNTTGG	2 cut(s)	501, 533
AluBI	AGCT	2 cut(s)	419, 737
AluI	AGCT	2 cut(s)	419, 737
Alw26I	GTCTC	1 cut(s)	993
AlwI	GGATC	4 cut(s)	408, 548, 635, 874
AlwNI	CAGNNNCTG	1 cut(s)	280
Aor51HI	AGCGCT	1 cut(s)	529
AoxI	GGCC	4 cut(s)	52, 90, 301, 778
ApoI	RAATTY	6 cut(s)	127, 217, 624, 941, 959, 1300
AseI	ATTAAT	2 cut(s)	207, 665
Asp700I	GAANNNNTTC	1 cut(s)	942
AspLEI	GCGC	2 cut(s)	530, 1151
AspS9I	GGNCC	4 cut(s)	301, 778, 797, 1280
AsuHPI	GGTGA	3 cut(s)	298, 713, 1021
AvaII	GGWCC	2 cut(s)	797, 1280
BbsI	GAAGAC	2 cut(s)	488, 999
BccI	CCATC	5 cut(s)	182, 226, 565, 771, 1078
BceAI	ACGGC	1 cut(s)	352
BcgI	CGANNNNNNTGC	2 cut(s)	329, 363
BciT130I	CCWGG	2 cut(s)	366, 1159
BcoDI	GTCTC	1 cut(s)	993
BfaI	CTAG	2 cut(s)	420, 1269
BfmI	CTRYAG	1 cut(s)	18
BfoI	RGCGCY	2 cut(s)	531, 1152
BfuAI	ACCTGC	1 cut(s)	1251
Bme1390I	CCNGG	2 cut(s)	366, 1159
Bme18I	GGWCC	2 cut(s)	797, 1280
BmgBI	CACGTC	1 cut(s)	1108
BmgT120I	GGNCC	4 cut(s)	301, 778, 797, 1280
BmiI	GGNNCC	2 cut(s)	13, 929
BmrFI	CCNGG	2 cut(s)	366, 1159
BmsI	GCATC	5 cut(s)	89, 441, 682, 851, 966
BpiI	GAAGAC	2 cut(s)	488, 999
BpmI	CTGGAG	2 cut(s)	338, 1161
BsaBI	GATNNNNATC	1 cut(s)	79
BsaJI	CCNNGG	3 cut(s)	263, 364, 1214
Bsc4I	CCNNNNNNNGG	4 cut(s)	71, 315, 322, 1057
Bse118I	RCCGGY	1 cut(s)	287
Bse1I	ACTGG	2 cut(s)	230, 1135
Bse8I	GATNNNNATC	1 cut(s)	79
BseBI	CCWGG	2 cut(s)	366, 1159
BseDI	CCNNGG	3 cut(s)	263, 364, 1214
BseGI	GGATG	4 cut(s)	193, 253, 842, 981
BseJI	GATNNNNATC	1 cut(s)	79
BseLI	CCNNNNNNNGG	4 cut(s)	71, 315, 322, 1057
BseMII	CTCAG	5 cut(s)	188, 500, 870, 1088, 1221
BseNI	ACTGG	2 cut(s)	230, 1135
BsgI	GTGCAG	1 cut(s)	1279
Bsh1236I	CGCG	1 cut(s)	656
BshFI	GGCC	4 cut(s)	54, 92, 303, 780
BsiSI	CCGG	2 cut(s)	288, 316
BslFI	GGGAC	1 cut(s)	184
BslI	CCNNNNNNNGG	4 cut(s)	71, 315, 322, 1057
BsmAI	GTCTC	1 cut(s)	993
BsmFI	GGGAC	1 cut(s)	184
BsnI	GGCC	4 cut(s)	54, 92, 303, 780
Bsp143I	GATC	5 cut(s)	3, 400, 553, 640, 879
BspACI	CCGC	1 cut(s)	477
BspANI	GGCC	4 cut(s)	54, 92, 303, 780
BspCNI	CTCAG	5 cut(s)	187, 499, 869, 1087, 1222
BspFNI	CGCG	1 cut(s)	656
BspLI	GGNNCC	2 cut(s)	13, 929
BspMAI	CTGCAG	1 cut(s)	22
BspMI	ACCTGC	1 cut(s)	1251
BspPI	GGATC	4 cut(s)	408, 548, 635, 874
BsrFI	RCCGGY	1 cut(s)	287
BsrI	ACTGG	2 cut(s)	230, 1135
BssAI	RCCGGY	1 cut(s)	287
BssECI	CCNNGG	3 cut(s)	263, 364, 1214
BssMI	GATC	5 cut(s)	3, 400, 553, 640, 879
Bst2UI	CCWGG	2 cut(s)	366, 1159
Bst4CI	ACNGT	4 cut(s)	310, 775, 823, 1329
BstAPI	GCANNNNNTGC	1 cut(s)	280
BstC8I	GCNNGC	3 cut(s)	285, 468, 1147
BstDEI	CTNAG	6 cut(s)	174, 451, 486, 856, 1074, 1230
BstDSI	CCRYGG	1 cut(s)	1214
BstF5I	GGATG	4 cut(s)	193, 253, 842, 981
BstFNI	CGCG	1 cut(s)	656
BstH2I	RGCGCY	2 cut(s)	531, 1152
BstHHI	GCGC	2 cut(s)	530, 1151
BstKTI	GATC	5 cut(s)	6, 403, 556, 643, 882
BstMAI	GTCTC	1 cut(s)	993
BstMBI	GATC	5 cut(s)	3, 400, 553, 640, 879
BstMWI	GCNNNNNNNGC	2 cut(s)	259, 280
BstNI	CCWGG	2 cut(s)	366, 1159
BstNSI	RCATGY	1 cut(s)	106
BstSCI	CCNGG	2 cut(s)	364, 1157
BstSFI	CTRYAG	1 cut(s)	18
BstUI	CGCG	1 cut(s)	656
BstV2I	GAAGAC	2 cut(s)	488, 999
BstX2I	RGATCY	1 cut(s)	879
BstXI	CCANNNNNNTGG	1 cut(s)	837
BstYI	RGATCY	1 cut(s)	879
BsuRI	GGCC	4 cut(s)	54, 92, 303, 780
BtgI	CCRYGG	1 cut(s)	1214
BtrI	CACGTC	1 cut(s)	1108
BtsCI	GGATG	4 cut(s)	193, 253, 842, 981
BveI	ACCTGC	1 cut(s)	1251
Cac8I	GCNNGC	3 cut(s)	285, 468, 1147
CaiI	CAGNNNCTG	1 cut(s)	280
CfoI	GCGC	2 cut(s)	530, 1151
Cfr10I	RCCGGY	1 cut(s)	287
Cfr13I	GGNCC	4 cut(s)	301, 778, 797, 1280
Csp6I	GTAC	1 cut(s)	1234
CviAII	CATG	6 cut(s)	103, 181, 404, 504, 932, 1024
CviQI	GTAC	1 cut(s)	1234
DdeI	CTNAG	6 cut(s)	174, 451, 486, 856, 1074, 1230
DpnI	GATC	5 cut(s)	5, 402, 555, 642, 881
DpnII	GATC	5 cut(s)	3, 400, 553, 640, 879
Eco47I	GGWCC	2 cut(s)	797, 1280
Eco47III	AGCGCT	1 cut(s)	529
Eco57I	CTGAAG	1 cut(s)	681
EcoRI	GAATTC	2 cut(s)	217, 624
EcoRII	CCWGG	2 cut(s)	364, 1157
FaeI	CATG	6 cut(s)	106, 184, 407, 507, 935, 1027
FaqI	GGGAC	1 cut(s)	184
FatI	CATG	6 cut(s)	102, 180, 403, 503, 931, 1023
FauI	CCCGC	1 cut(s)	470
FauNDI	CATATG	1 cut(s)	472
FblI	GTMKAC	1 cut(s)	966
FokI	GGATG	4 cut(s)	200, 260, 829, 988
FspBI	CTAG	2 cut(s)	420, 1269
GlaI	GCGC	2 cut(s)	529, 1150
GsuI	CTGGAG	2 cut(s)	338, 1161
HaeII	RGCGCY	2 cut(s)	531, 1152
HaeIII	GGCC	4 cut(s)	54, 92, 303, 780
HapII	CCGG	2 cut(s)	288, 316
HhaI	GCGC	2 cut(s)	530, 1151
Hin1II	CATG	6 cut(s)	106, 184, 407, 507, 935, 1027
Hin6I	GCGC	2 cut(s)	528, 1149
HinP1I	GCGC	2 cut(s)	528, 1149
HincII	GTYRAC	1 cut(s)	819
HindII	GTYRAC	1 cut(s)	819
HinfI	GANTC	8 cut(s)	75, 172, 484, 539, 592, 826, 849, 1272
HpaI	GTTAAC	1 cut(s)	819
HpaII	CCGG	2 cut(s)	288, 316
HphI	GGTGA	3 cut(s)	298, 713, 1021
Hpy166II	GTNNAC	3 cut(s)	819, 967, 1029
Hpy188I	TCNGA	2 cut(s)	859, 1231
Hpy188III	TCNNGA	6 cut(s)	355, 577, 589, 830, 846, 1140
Hpy8I	GTNNAC	3 cut(s)	819, 967, 1029
HpyAV	CCTTC	3 cut(s)	103, 506, 779
HpyCH4III	ACNGT	4 cut(s)	310, 775, 823, 1329
HpyCH4IV	ACGT	2 cut(s)	585, 1107
HpyCH4V	TGCA	5 cut(s)	20, 283, 466, 684, 1260
HpyF10VI	GCNNNNNNNGC	2 cut(s)	259, 280
HpyF3I	CTNAG	6 cut(s)	174, 451, 486, 856, 1074, 1230
HpySE526I	ACGT	2 cut(s)	585, 1107
Hsp92II	CATG	6 cut(s)	106, 184, 407, 507, 935, 1027
HspAI	GCGC	2 cut(s)	528, 1149
KspAI	GTTAAC	1 cut(s)	819
Kzo9I	GATC	5 cut(s)	3, 400, 553, 640, 879
LmnI	GCTCC	1 cut(s)	1142
LweI	GCATC	5 cut(s)	89, 441, 682, 851, 966
MaeI	CTAG	2 cut(s)	420, 1269
MaeII	ACGT	2 cut(s)	585, 1107
MaeIII	GTNAC	3 cut(s)	113, 368, 701
MalI	GATC	5 cut(s)	5, 402, 555, 642, 881
MboI	GATC	5 cut(s)	3, 400, 553, 640, 879
MboII	GAAGA	9 cut(s)	37, 40, 493, 521, 541, 619, 737, 950, 1004
MfeI	CAATTG	1 cut(s)	750
MflI	RGATCY	1 cut(s)	879
MluCI	AATT	9 cut(s)	127, 217, 624, 662, 750, 941, 959, 1209, 1300
MlyI	GAGTC	6 cut(s)	166, 478, 533, 586, 843, 1266
MmeI	TCCRAC	2 cut(s)	165, 1153
MnlI	CCTC	8 cut(s)	25, 156, 258, 314, 362, 687, 910, 1169
MroXI	GAANNNNTTC	1 cut(s)	942
MseI	TTAA	6 cut(s)	207, 330, 456, 665, 818, 957
MslI	CAYNNNNRTG	2 cut(s)	101, 375
MspI	CCGG	2 cut(s)	288, 316
MspR9I	CCNGG	2 cut(s)	366, 1159
MunI	CAATTG	1 cut(s)	750
MvaI	CCWGG	2 cut(s)	366, 1159
MvnI	CGCG	1 cut(s)	656
MwoI	GCNNNNNNNGC	2 cut(s)	259, 280
NdeI	CATATG	1 cut(s)	472
NdeII	GATC	5 cut(s)	3, 400, 553, 640, 879
NlaIII	CATG	6 cut(s)	106, 184, 407, 507, 935, 1027
NlaIV	GGNNCC	2 cut(s)	13, 929
NmeAIII	GCCGAG	2 cut(s)	288, 831
NmuCI	GTSAC	2 cut(s)	368, 701
NspI	RCATGY	1 cut(s)	106
PciI	ACATGT	1 cut(s)	102
PdmI	GAANNNNTTC	1 cut(s)	942
PfeI	GAWTC	2 cut(s)	75, 826
PleI	GAGTC	6 cut(s)	166, 478, 533, 586, 843, 1266
PpsI	GAGTC	6 cut(s)	166, 478, 533, 586, 843, 1266
PscI	ACATGT	1 cut(s)	102
PshBI	ATTAAT	2 cut(s)	207, 665
Psp6I	CCWGG	2 cut(s)	364, 1157
PspGI	CCWGG	2 cut(s)	364, 1157
PspN4I	GGNNCC	2 cut(s)	13, 929
PspPI	GGNCC	4 cut(s)	301, 778, 797, 1280
PstI	CTGCAG	1 cut(s)	22
PstNI	CAGNNNCTG	1 cut(s)	280
PsuI	RGATCY	1 cut(s)	879
RsaI	GTAC	1 cut(s)	1235
RsaNI	GTAC	1 cut(s)	1234
RseI	CAYNNNNRTG	2 cut(s)	101, 375
SaqAI	TTAA	6 cut(s)	207, 330, 456, 665, 818, 957
Sau3AI	GATC	5 cut(s)	3, 400, 553, 640, 879
Sau96I	GGNCC	4 cut(s)	301, 778, 797, 1280
SchI	GAGTC	6 cut(s)	166, 478, 533, 586, 843, 1266
ScrFI	CCNGG	2 cut(s)	366, 1159
SfaNI	GCATC	5 cut(s)	89, 441, 682, 851, 966
SfcI	CTRYAG	1 cut(s)	18
SinI	GGWCC	2 cut(s)	797, 1280
SmiMI	CAYNNNNRTG	2 cut(s)	101, 375
Sse9I	AATT	9 cut(s)	127, 217, 624, 662, 750, 941, 959, 1209, 1300
SsiI	CCGC	1 cut(s)	477
SspI	AATATT	1 cut(s)	889
SspMI	CTAG	2 cut(s)	420, 1269
StyD4I	CCNGG	2 cut(s)	364, 1157
TaaI	ACNGT	4 cut(s)	310, 775, 823, 1329
TaiI	ACGT	2 cut(s)	588, 1110
TaqI	TCGA	2 cut(s)	125, 552
TasI	AATT	9 cut(s)	127, 217, 624, 662, 750, 941, 959, 1209, 1300
TatI	WGTACW	1 cut(s)	1233
TfiI	GAWTC	2 cut(s)	75, 826
Tru1I	TTAA	6 cut(s)	207, 330, 456, 665, 818, 957
Tru9I	TTAA	6 cut(s)	207, 330, 456, 665, 818, 957
TseFI	GTSAC	2 cut(s)	368, 701
Tsp45I	GTSAC	2 cut(s)	368, 701
TspDTI	ATGAA	5 cut(s)	85, 169, 896, 951, 1020
TspGWI	ACGGA	3 cut(s)	1011, 1203, 1215
VpaK11BI	GGWCC	2 cut(s)	797, 1280
VspI	ATTAAT	2 cut(s)	207, 665
XapI	RAATTY	6 cut(s)	127, 217, 624, 941, 959, 1300
XceI	RCATGY	1 cut(s)	106
XcmI	CCANNNNNNNNNTGG	1 cut(s)	362
XmiI	GTMKAC	1 cut(s)	966
XmnI	GAANNNNTTC	1 cut(s)	942
XspI	CTAG	2 cut(s)	420, 1269

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.