Rorug02G0264100

Belongs to the glycosyl hydrolase 5 (cellulase A) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0009537
Physical Location & Seq
Reverse (-)
27880065 .. 27884659
4595 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0264100.1

Sequence Viewer

Length: 996 bp
ATGAGTGCCGGCAAAGGTGATAGGAAGGCGACTCTTGACGCCGCCTCATGGCTCTTCAATGTGGTTACATCTGTTGGAATAATCATTGTCAATAAATCATTGATGGCTACGCATGGTTTCAGTTTCGCCACAACGTTAACGGGTCTGCATTTTGCCACAACAACCTTATTGACACTTGTCCTTAAGTGGCTGGGATACATCCAGCCTTCCCAGTTACCACTGCCTGAGCTTTTGAAGTTTGTTTTATTTGCAAATTTCTCCATAGTTGGGATGAATGTGAGCTTAATGTGGAACTCTGTTGGATTCTATCAGATCGCGAAGCTGACTATGATCCCAGTATCATGTTTTCTAGAGGTTGTCTTGGACAAGGTCCGATACTCAAGGGATACGAAGCTTAGCATAACATTGGTTCTCCTTGGTGTTGCAGTTTGTACTGTTACTGATGTGAGTGTTAATGCCAAGGGTTTTATAGCTGCTTCAGTGGCAGTTTGGAGCACTGCCCTACAACAGTATTACGTGCACCATCTTCAACGGAAGTATTCTCTTAGTTCTTTCAACCTATTGGGACATACTGCACCAGTACAGGCTGCATCTTTGCTGATATTAGGTCCCTTCGTGGACTTCTGGTTGACAAGCAAAACAGTTTATGCATATGACTATCATTTTGTATCAGTGTCATTGATTCTCCTATCTTGCACCATTGCGGTAGGAACCAACCTCAGCCAGTTCATATGCATCGGCAGATTTACTGCTGTGTCATTTCAAGTCCTGGGCCATATGAAGACGATTCTAGTCTTGATCTTAGGATTCATATTCTTTGGGAAAGAGGGTCTCAATCATCAAGTAGTTTTAGGTATGTTCATTGCAATCATGGGAATGGTATGGTATGGCAATGCCTCATCCAAACCAGGAGGCAAGGAGCGTCGTTCCGCTCCAAGCAAATCCCAGAAGAAAGGCACTGTATCAGAGTCCTCTGAAGTGGATGACAAGGTATAG

Protein Analysis

331

Amino Acids

36.16

Weight (kDa)

9.5

Isoelectric Point (pI)

28.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPT PF03151 22 - 296 3.8e-15 Triose-phosphate Transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 932
AccII CGCG 1 cut(s) 317
AciI CCGC 3 cut(s) 42, 704, 930
AclI AACGTT 1 cut(s) 134
AclWI GGATC 1 cut(s) 325
AcsI RAATTY 1 cut(s) 253
AcuI CTGAAG 2 cut(s) 462, 996
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 2 cut(s) 433, 582
AfiI CCNNNNNNNGG 2 cut(s) 48, 267
AflII CTTAAG 1 cut(s) 182
AgsI TTSAA 5 cut(s) 58, 235, 530, 556, 764
AjnI CCWGG 2 cut(s) 768, 907
AluBI AGCT 5 cut(s) 229, 282, 322, 394, 473
AluI AGCT 5 cut(s) 229, 282, 322, 394, 473
Alw21I GWGCWC 2 cut(s) 497, 522
Alw26I GTCTC 1 cut(s) 836
Alw44I GTGCAC 1 cut(s) 518
AlwI GGATC 1 cut(s) 325
AoxI GGCC 1 cut(s) 772
ApaLI GTGCAC 1 cut(s) 518
ApeKI GCWGC 2 cut(s) 473, 587
ApoI RAATTY 1 cut(s) 253
ArsI GACNNNNNNTTYG 2 cut(s) 647, 679
AspS9I GGNCC 3 cut(s) 370, 608, 772
AsuHPI GGTGA 1 cut(s) 29
AvaII GGWCC 2 cut(s) 370, 608
BaeGI GKGCMC 1 cut(s) 522
BbsI GAAGAC 1 cut(s) 788
Bbv12I GWGCWC 2 cut(s) 497, 522
BbvCI CCTCAGC 1 cut(s) 719
BbvI GCAGC 2 cut(s) 460, 574
BccI CCATC 2 cut(s) 97, 531
BciT130I CCWGG 2 cut(s) 770, 909
BciVI GTATCC 2 cut(s) 188, 379
BcoDI GTCTC 1 cut(s) 836
BfaI CTAG 2 cut(s) 350, 791
BfrI CTTAAG 1 cut(s) 182
BfuI GTATCC 2 cut(s) 188, 379
BisI GCNGC 3 cut(s) 42, 474, 588
BlpI GCTNAGC 1 cut(s) 395
BlsI GCNGC 3 cut(s) 43, 475, 589
Bme1390I CCNGG 2 cut(s) 770, 909
Bme18I GGWCC 2 cut(s) 370, 608
BmgT120I GGNCC 3 cut(s) 370, 608, 772
BmiI GGNNCC 2 cut(s) 610, 712
BmrFI CCNGG 2 cut(s) 770, 909
BmrI ACTGGG 2 cut(s) 205, 329
BmsI GCATC 2 cut(s) 599, 744
BmuI ACTGGG 2 cut(s) 205, 329
BoxI GACNNNNGTC 1 cut(s) 176
BpiI GAAGAC 1 cut(s) 788
Bpu10I CCTNAGC 2 cut(s) 225, 719
Bpu1102I GCTNAGC 1 cut(s) 395
BpuEI CTTGAG 1 cut(s) 364
BsaAI YACGTR 1 cut(s) 517
BsaHI GRCGYC 1 cut(s) 39
BsaI GGTCTC 1 cut(s) 836
BsaJI CCNNGG 3 cut(s) 415, 459, 769
Bsc4I CCNNNNNNNGG 2 cut(s) 48, 267
Bse118I RCCGGY 1 cut(s) 8
Bse1I ACTGG 4 cut(s) 211, 335, 578, 724
Bse3DI GCAATG 3 cut(s) 699, 861, 898
BseBI CCWGG 2 cut(s) 770, 909
BseDI CCNNGG 3 cut(s) 415, 459, 769
BseGI GGATG 4 cut(s) 198, 276, 899, 988
BseLI CCNNNNNNNGG 2 cut(s) 48, 267
BseMI GCAATG 3 cut(s) 699, 861, 898
BseMII CTCAG 2 cut(s) 216, 733
BseNI ACTGG 4 cut(s) 211, 335, 578, 724
BseSI GKGCMC 1 cut(s) 522
BseXI GCAGC 2 cut(s) 460, 574
BseYI CCCAGC 1 cut(s) 190
BsgI GTGCAG 1 cut(s) 558
Bsh1236I CGCG 1 cut(s) 317
BshFI GGCC 1 cut(s) 774
BsiHKAI GWGCWC 2 cut(s) 497, 522
BsiSI CCGG 1 cut(s) 9
BslFI GGGAC 2 cut(s) 579, 594
BslI CCNNNNNNNGG 2 cut(s) 48, 267
BsmAI GTCTC 1 cut(s) 836
BsmFI GGGAC 2 cut(s) 579, 594
BsnI GGCC 1 cut(s) 774
Bso31I GGTCTC 1 cut(s) 836
Bsp1286I GDGCHC 2 cut(s) 497, 522
Bsp143I GATC 3 cut(s) 312, 330, 798
Bsp1720I GCTNAGC 1 cut(s) 395
Bsp68I TCGCGA 1 cut(s) 317
BspACI CCGC 3 cut(s) 42, 704, 930
BspANI GGCC 1 cut(s) 774
BspCNI CTCAG 2 cut(s) 217, 732
BspFNI CGCG 1 cut(s) 317
BspLI GGNNCC 2 cut(s) 610, 712
BspPI GGATC 1 cut(s) 325
BspQI GCTCTTC 1 cut(s) 59
BspTI CTTAAG 1 cut(s) 182
BspTNI GGTCTC 1 cut(s) 836
BsrBI CCGCTC 1 cut(s) 932
BsrDI GCAATG 3 cut(s) 699, 861, 898
BsrFI RCCGGY 1 cut(s) 8
BsrI ACTGG 4 cut(s) 211, 335, 578, 724
BssAI RCCGGY 1 cut(s) 8
BssECI CCNNGG 3 cut(s) 415, 459, 769
BssMI GATC 3 cut(s) 312, 330, 798
BssNI GRCGYC 1 cut(s) 39
BssT1I CCWWGG 2 cut(s) 415, 459
Bst2UI CCWGG 2 cut(s) 770, 909
Bst4CI ACNGT 4 cut(s) 436, 510, 643, 961
Bst6I CTCTTC 1 cut(s) 59
BstACI GRCGYC 1 cut(s) 39
BstAFI CTTAAG 1 cut(s) 182
BstBAI YACGTR 1 cut(s) 517
BstC8I GCNNGC 1 cut(s) 10
BstDEI CTNAG 5 cut(s) 225, 395, 545, 719, 802
BstF5I GGATG 4 cut(s) 198, 276, 899, 988
BstFNI CGCG 1 cut(s) 317
BstKTI GATC 3 cut(s) 315, 333, 801
BstMAI GTCTC 1 cut(s) 836
BstMBI GATC 3 cut(s) 312, 330, 798
BstMWI GCNNNNNNNGC 1 cut(s) 482
BstNI CCWGG 2 cut(s) 770, 909
BstPAI GACNNNNGTC 1 cut(s) 176
BstSCI CCNGG 2 cut(s) 768, 907
BstSLI GKGCMC 1 cut(s) 522
BstUI CGCG 1 cut(s) 317
BstV1I GCAGC 2 cut(s) 460, 574
BstV2I GAAGAC 1 cut(s) 788
BsuI GTATCC 2 cut(s) 188, 379
BsuRI GGCC 1 cut(s) 774
BtsCI GGATG 4 cut(s) 198, 276, 899, 988
BtsI GCAGTG 2 cut(s) 218, 495
BtsIMutI CAGTG 5 cut(s) 218, 486, 495, 678, 957
BtuMI TCGCGA 1 cut(s) 317
Cac8I GCNNGC 1 cut(s) 10
Cfr10I RCCGGY 1 cut(s) 8
Cfr13I GGNCC 3 cut(s) 370, 608, 772
CseI GACGC 2 cut(s) 47, 911
Csp6I GTAC 2 cut(s) 432, 581
CviAII CATG 4 cut(s) 48, 113, 342, 871
CviQI GTAC 2 cut(s) 432, 581
DdeI CTNAG 5 cut(s) 225, 395, 545, 719, 802
DpnI GATC 3 cut(s) 314, 332, 800
DpnII GATC 3 cut(s) 312, 330, 798
Eam1104I CTCTTC 1 cut(s) 59
EarI CTCTTC 1 cut(s) 59
Eco130I CCWWGG 2 cut(s) 415, 459
Eco31I GGTCTC 1 cut(s) 836
Eco47I GGWCC 2 cut(s) 370, 608
Eco57I CTGAAG 2 cut(s) 462, 996
EcoO109I RGGNCCY 1 cut(s) 608
EcoRII CCWGG 2 cut(s) 768, 907
EcoT14I CCWWGG 2 cut(s) 415, 459
EcoT22I ATGCAT 2 cut(s) 652, 737
ErhI CCWWGG 2 cut(s) 415, 459
FaeI CATG 4 cut(s) 51, 116, 345, 874
FaqI GGGAC 2 cut(s) 579, 594
FatI CATG 4 cut(s) 47, 112, 341, 870
FauNDI CATATG 3 cut(s) 652, 731, 777
Fnu4HI GCNGC 3 cut(s) 42, 474, 588
FokI GGATG 3 cut(s) 185, 283, 886
Fsp4HI GCNGC 3 cut(s) 42, 474, 588
FspBI CTAG 2 cut(s) 350, 791
GluI GCNGC 3 cut(s) 42, 474, 588
GsaI CCCAGC 1 cut(s) 194
HaeIII GGCC 1 cut(s) 774
HapII CCGG 1 cut(s) 9
HgaI GACGC 2 cut(s) 47, 911
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 4 cut(s) 51, 116, 345, 874
HincII GTYRAC 2 cut(s) 138, 630
HindII GTYRAC 2 cut(s) 138, 630
HindIII AAGCTT 1 cut(s) 392
HinfI GANTC 6 cut(s) 31, 303, 682, 787, 807, 968
HpaI GTTAAC 1 cut(s) 138
HpaII CCGG 1 cut(s) 9
HphI GGTGA 1 cut(s) 29
Hpy166II GTNNAC 4 cut(s) 138, 520, 619, 630
Hpy188I TCNGA 4 cut(s) 312, 374, 967, 976
Hpy188III TCNNGA 4 cut(s) 35, 316, 350, 796
Hpy8I GTNNAC 4 cut(s) 138, 520, 619, 630
Hpy99I CGWCG 1 cut(s) 927
HpyAV CCTTC 3 cut(s) 19, 216, 622
HpyCH4III ACNGT 4 cut(s) 436, 510, 643, 961
HpyCH4IV ACGT 2 cut(s) 134, 516
HpyF10VI GCNNNNNNNGC 1 cut(s) 482
HpyF3I CTNAG 5 cut(s) 225, 395, 545, 719, 802
HpySE526I ACGT 2 cut(s) 134, 516
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 4 cut(s) 51, 116, 345, 874
KroI GCCGGC 1 cut(s) 8
KroNI GCCGGC 1 cut(s) 10
KspAI GTTAAC 1 cut(s) 138
Kzo9I GATC 3 cut(s) 312, 330, 798
LguI GCTCTTC 1 cut(s) 59
LmnI GCTCC 3 cut(s) 492, 919, 937
Lsp1109I GCAGC 2 cut(s) 460, 574
LweI GCATC 2 cut(s) 599, 744
MaeI CTAG 2 cut(s) 350, 791
MaeII ACGT 2 cut(s) 134, 516
MaeIII GTNAC 3 cut(s) 64, 213, 436
MalI GATC 3 cut(s) 314, 332, 800
MbiI CCGCTC 1 cut(s) 932
MboI GATC 3 cut(s) 312, 330, 798
MboII GAAGA 4 cut(s) 46, 518, 793, 961
MhlI GDGCHC 2 cut(s) 497, 522
MluCI AATT 1 cut(s) 253
MlyI GAGTC 2 cut(s) 25, 977
MmeI TCCRAC 2 cut(s) 55, 280
MnlI CCTC 7 cut(s) 55, 346, 728, 820, 905, 907, 982
Mph1103I ATGCAT 2 cut(s) 652, 737
MroNI GCCGGC 1 cut(s) 8
MseI TTAA 4 cut(s) 137, 183, 284, 453
MslI CAYNNNNRTG 1 cut(s) 875
MspCI CTTAAG 1 cut(s) 182
MspI CCGG 1 cut(s) 9
MspR9I CCNGG 2 cut(s) 770, 909
MvaI CCWGG 2 cut(s) 770, 909
MvnI CGCG 1 cut(s) 317
MwoI GCNNNNNNNGC 1 cut(s) 482
NaeI GCCGGC 1 cut(s) 10
NdeI CATATG 3 cut(s) 652, 731, 777
NdeII GATC 3 cut(s) 312, 330, 798
NgoMIV GCCGGC 1 cut(s) 8
NlaIII CATG 4 cut(s) 51, 116, 345, 874
NlaIV GGNNCC 2 cut(s) 610, 712
NruI TCGCGA 1 cut(s) 317
NsiI ATGCAT 2 cut(s) 652, 737
PciSI GCTCTTC 1 cut(s) 59
PdiI GCCGGC 1 cut(s) 10
PfeI GAWTC 4 cut(s) 303, 682, 787, 807
PflFI GACNNNGTC 1 cut(s) 368
PkrI GCNGC 3 cut(s) 43, 475, 589
PleI GAGTC 2 cut(s) 25, 976
PpsI GAGTC 2 cut(s) 25, 976
Ppu21I YACGTR 1 cut(s) 517
PpuMI RGGWCCY 1 cut(s) 608
PshAI GACNNNNGTC 1 cut(s) 176
Psp1406I AACGTT 1 cut(s) 134
Psp5II RGGWCCY 1 cut(s) 608
Psp6I CCWGG 2 cut(s) 768, 907
PspFI CCCAGC 1 cut(s) 190
PspGI CCWGG 2 cut(s) 768, 907
PspN4I GGNNCC 2 cut(s) 610, 712
PspPI GGNCC 3 cut(s) 370, 608, 772
PspPPI RGGWCCY 1 cut(s) 608
PsyI GACNNNGTC 1 cut(s) 368
RruI TCGCGA 1 cut(s) 317
RsaI GTAC 2 cut(s) 433, 582
RsaNI GTAC 2 cut(s) 432, 581
RseI CAYNNNNRTG 1 cut(s) 875
SapI GCTCTTC 1 cut(s) 59
SaqAI TTAA 4 cut(s) 137, 183, 284, 453
SatI GCNGC 3 cut(s) 42, 474, 588
Sau3AI GATC 3 cut(s) 312, 330, 798
Sau96I GGNCC 3 cut(s) 370, 608, 772
SchI GAGTC 2 cut(s) 25, 977
ScrFI CCNGG 2 cut(s) 770, 909
SduI GDGCHC 2 cut(s) 497, 522
SfaNI GCATC 2 cut(s) 599, 744
SinI GGWCC 2 cut(s) 370, 608
SmiMI CAYNNNNRTG 1 cut(s) 875
SmlI CTYRAG 2 cut(s) 182, 379
SmoI CTYRAG 2 cut(s) 182, 379
Sse9I AATT 1 cut(s) 253
SsiI CCGC 3 cut(s) 42, 704, 930
SspMI CTAG 2 cut(s) 350, 791
StyD4I CCNGG 2 cut(s) 768, 907
StyI CCWWGG 2 cut(s) 415, 459
TaaI ACNGT 4 cut(s) 436, 510, 643, 961
TaiI ACGT 2 cut(s) 137, 519
TasI AATT 1 cut(s) 253
TatI WGTACW 2 cut(s) 431, 580
TauI GCSGC 1 cut(s) 44
TfiI GAWTC 4 cut(s) 303, 682, 787, 807
Tru1I TTAA 4 cut(s) 137, 183, 284, 453
Tru9I TTAA 4 cut(s) 137, 183, 284, 453
TscAI CASTG 5 cut(s) 225, 486, 502, 678, 964
TseI GCWGC 2 cut(s) 473, 587
TspDTI ATGAA 5 cut(s) 287, 718, 794, 799, 850
TspGWI ACGGA 1 cut(s) 547
TspRI CASTG 5 cut(s) 225, 486, 502, 678, 964
Tth111I GACNNNGTC 1 cut(s) 368
Vha464I CTTAAG 1 cut(s) 182
VneI GTGCAC 1 cut(s) 518
VpaK11BI GGWCC 2 cut(s) 370, 608
XapI RAATTY 1 cut(s) 253
XbaI TCTAGA 1 cut(s) 349
XspI CTAG 2 cut(s) 350, 791
Zsp2I ATGCAT 2 cut(s) 652, 737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.