Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0013890

Physical Location & Seq

Genomic Coordinates Forward (+)

33407349 .. 33409236

Gene Structure 1888 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1272100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 648 bp

ATGGTTGTCAAAGTATATGGTCCAGTAATGGCAGCCTGCCCCCAAAGGGTGATGGTTTGCCTTTTGGAGAAAGGAGTGAACTTTGAAATTGTAGATGTTGATCTTGAGGCAGGAGAGCACAAGCGACCCGAGTTTCTCACCCGTCAGCCGTTTGGGCAAGTTCCAGTTGTAGAAGATGGTGACTTCAGGCTCTTTGAATCTAGGGCGATCATAAGGTACTACGCAGCCAAGTATGCAGGCCGTGGTCCCAACCTATTGGGAACAACTCTGGAGGAGAAAGCAGTTGTTGACCAGTGGCTGGAAGTGGAAGGCCACAACTTCAACGACCTGGTTTACACTCTGGTGCTCCAGCTTCTTGTCCTGCCACGGATGGGTCAACGTGGCGACGCTGCATTAATCAATGCATGTGAGGAGAAACTGGAGAAGGTGTTTGATGTTTATGAGGAAAGATTGTCAAAAAGCAAATATCTTGCTGGAGAAACTTTCACTCTGGCTGATCTGAGCCATCTTCCTGGGATTAGGTATCTGATTGATGAAGCTAAACTGGGGCATTTGGTGACTGGGAGGAAGAAAGTGAATGCATGGTGGGAGGACATATCCAACAGGCCTTCTTGGAAGAAACTAATGCAGCTTGCTAGTGACTACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0003674 GO:0003824 GO:0004364 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0006518 GO:0006575 GO:0006749 GO:0006790 GO:0006807 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009056 GO:0009404 GO:0009407 GO:0009636 GO:0009705 GO:0009812 GO:0009889 GO:0009962 GO:0009987 GO:0016020 GO:0016740 GO:0016765 GO:0019222 GO:0019748 GO:0031090 GO:0034641 GO:0042221 GO:0042440 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0044237 GO:0044248 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046283 GO:0050789 GO:0050896 GO:0051186 GO:0065007 GO:0071704 GO:0080090 GO:0098588 GO:0098754 GO:0098805 GO:1900384 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418

Pfam Domains

Protein Families

GST_C GST_N

Protein Analysis

216

Amino Acids

24.49

Weight (kDa)

5.67

Isoelectric Point (pI)

31.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GST_N	PF02798	1 - 73	4.1e-20	Glutathione S-transferase, N-terminal domain
GST_N_3	PF13417	10 - 81	9.5e-12	Glutathione S-transferase, N-terminal domain
GST_N_2	PF13409	13 - 75	8.4e-14	Glutathione S-transferase, N-terminal domain
GST_C	PF00043	114 - 203	6.8e-14	Glutathione S-transferase, C-terminal domain
GST_C_3	PF14497	135 - 206	5.9e-07	Glutathione S-transferase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0013890)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G03190 AT5G17220
fragaria_vesca	FvH4_1g27460
malus_domestica	MD17G1272100.v1.1
prunus_persica	Prupe.3G013600_v2.0.a1
pyrus_communis	pycom17g27080
rosa_chinensis	RchiOBHm_Chr3g0488551
rosa_laevigata	RLG00000022944
rosa_multiflora	Rmu_co8213432.1_g000001 Rmu_sc0005363.1_g000020
rosa_roxburghii	Rroxscaffold_6G00394180
rosa_rugosa	Rorug03G0239200.1
rosa_samantha	Rh3AG288000 Rh3BG324700 Rh3CG322100 Rh3DG320800
rosa_wichuraiana	Rw3G025500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	298
AcuI	CTGAAG	1 cut(s)	169
AfaI	GTAC	1 cut(s)	218
AfiI	CCNNNNNNNGG	3 cut(s)	46, 298, 371
AgsI	TTSAA	3 cut(s)	86, 197, 322
AjnI	CCWGG	2 cut(s)	327, 511
AleI	CACNNNNGTG	1 cut(s)	341
AluBI	AGCT	3 cut(s)	352, 539, 631
AluI	AGCT	3 cut(s)	352, 539, 631
Alw21I	GWGCWC	2 cut(s)	120, 348
AlwNI	CAGNNNCTG	1 cut(s)	298
Ama87I	CYCGRG	1 cut(s)	128
AoxI	GGCC	3 cut(s)	238, 310, 605
ApeKI	GCWGC	4 cut(s)	32, 224, 389, 628
AseI	ATTAAT	1 cut(s)	395
AspS9I	GGNCC	2 cut(s)	20, 245
AsuHPI	GGTGA	4 cut(s)	61, 130, 191, 568
AvaI	CYCGRG	1 cut(s)	128
AvaII	GGWCC	2 cut(s)	20, 245
Bbv12I	GWGCWC	2 cut(s)	120, 348
BbvI	GCAGC	4 cut(s)	44, 236, 376, 640
BccI	CCATC	4 cut(s)	46, 170, 364, 513
BceAI	ACGGC	2 cut(s)	133, 225
BciT130I	CCWGG	2 cut(s)	329, 513
BfaI	CTAG	3 cut(s)	201, 636, 646
BglI	GCCNNNNNGGC	1 cut(s)	154
BisI	GCNGC	4 cut(s)	33, 225, 390, 629
BlsI	GCNGC	4 cut(s)	34, 226, 391, 630
Bme1390I	CCNGG	2 cut(s)	329, 513
Bme18I	GGWCC	2 cut(s)	20, 245
BmeT110I	CYCGRG	1 cut(s)	128
BmgT120I	GGNCC	2 cut(s)	20, 245
BmiI	GGNNCC	1 cut(s)	247
BmrFI	CCNGG	2 cut(s)	329, 513
BmrI	ACTGGG	2 cut(s)	554, 570
BmuI	ACTGGG	2 cut(s)	554, 570
BpmI	CTGGAG	4 cut(s)	290, 332, 440, 495
BpuEI	CTTGAG	1 cut(s)	125
BsaBI	GATNNNNATC	1 cut(s)	99
BsaJI	CCNNGG	3 cut(s)	241, 365, 512
BsaXI	ACNNNNNCTCC	2 cut(s)	413, 443
Bsc4I	CCNNNNNNNGG	3 cut(s)	46, 298, 371
Bse1I	ACTGG	6 cut(s)	23, 164, 292, 423, 549, 565
Bse8I	GATNNNNATC	1 cut(s)	99
BseBI	CCWGG	2 cut(s)	329, 513
BseDI	CCNNGG	3 cut(s)	241, 365, 512
BseGI	GGATG	1 cut(s)	375
BseJI	GATNNNNATC	1 cut(s)	99
BseLI	CCNNNNNNNGG	3 cut(s)	46, 298, 371
BseMII	CTCAG	1 cut(s)	491
BseNI	ACTGG	6 cut(s)	23, 164, 292, 423, 549, 565
BseRI	GAGGAG	2 cut(s)	287, 425
BseXI	GCAGC	4 cut(s)	44, 236, 376, 640
BshFI	GGCC	3 cut(s)	240, 312, 607
BsiHKAI	GWGCWC	2 cut(s)	120, 348
BsiHKCI	CYCGRG	1 cut(s)	128
BslFI	GGGAC	1 cut(s)	231
BslI	CCNNNNNNNGG	3 cut(s)	46, 298, 371
BsmFI	GGGAC	1 cut(s)	231
BsmI	GAATGC	1 cut(s)	583
BsnI	GGCC	3 cut(s)	240, 312, 607
BsoBI	CYCGRG	1 cut(s)	128
Bsp1286I	GDGCHC	2 cut(s)	120, 348
Bsp143I	GATC	3 cut(s)	100, 207, 496
BspANI	GGCC	3 cut(s)	240, 312, 607
BspCNI	CTCAG	1 cut(s)	492
BspLI	GGNNCC	1 cut(s)	247
BsrI	ACTGG	6 cut(s)	23, 164, 292, 423, 549, 565
BssECI	CCNNGG	3 cut(s)	241, 365, 512
BssMI	GATC	3 cut(s)	100, 207, 496
Bst2UI	CCWGG	2 cut(s)	329, 513
BstC8I	GCNNGC	3 cut(s)	37, 238, 633
BstDEI	CTNAG	1 cut(s)	500
BstDSI	CCRYGG	2 cut(s)	241, 365
BstF5I	GGATG	1 cut(s)	375
BstKTI	GATC	3 cut(s)	103, 210, 499
BstMBI	GATC	3 cut(s)	100, 207, 496
BstMWI	GCNNNNNNNGC	2 cut(s)	154, 233
BstNI	CCWGG	2 cut(s)	329, 513
BstNSI	RCATGY	1 cut(s)	408
BstSCI	CCNGG	2 cut(s)	327, 511
BstV1I	GCAGC	4 cut(s)	44, 236, 376, 640
BstXI	CCANNNNNNTGG	2 cut(s)	256, 512
BsuRI	GGCC	3 cut(s)	240, 312, 607
BtgI	CCRYGG	2 cut(s)	241, 365
BtsCI	GGATG	1 cut(s)	375
BtsIMutI	CAGTG	1 cut(s)	299
Cac8I	GCNNGC	3 cut(s)	37, 238, 633
CaiI	CAGNNNCTG	1 cut(s)	298
Cfr13I	GGNCC	2 cut(s)	20, 245
CseI	GACGC	1 cut(s)	395
CsiI	ACCWGGT	1 cut(s)	327
Csp6I	GTAC	1 cut(s)	217
CviAII	CATG	2 cut(s)	405, 582
CviQI	GTAC	1 cut(s)	217
DdeI	CTNAG	1 cut(s)	500
DpnI	GATC	3 cut(s)	102, 209, 498
DpnII	GATC	3 cut(s)	100, 207, 496
Eco147I	AGGCCT	1 cut(s)	607
Eco47I	GGWCC	2 cut(s)	20, 245
Eco57I	CTGAAG	1 cut(s)	169
Eco88I	CYCGRG	1 cut(s)	128
EcoRII	CCWGG	2 cut(s)	327, 511
EcoT22I	ATGCAT	2 cut(s)	406, 583
FaeI	CATG	2 cut(s)	408, 585
FaiI	YATR	8 cut(s)	16, 18, 212, 234, 406, 441, 583, 596
FaqI	GGGAC	1 cut(s)	231
FatI	CATG	2 cut(s)	404, 581
Fnu4HI	GCNGC	4 cut(s)	33, 225, 390, 629
FokI	GGATG	1 cut(s)	382
Fsp4HI	GCNGC	4 cut(s)	33, 225, 390, 629
FspBI	CTAG	3 cut(s)	201, 636, 646
GluI	GCNGC	4 cut(s)	33, 225, 390, 629
GsuI	CTGGAG	4 cut(s)	290, 332, 440, 495
HaeIII	GGCC	3 cut(s)	240, 312, 607
HgaI	GACGC	1 cut(s)	395
Hin1II	CATG	2 cut(s)	408, 585
HincII	GTYRAC	2 cut(s)	289, 377
HindII	GTYRAC	2 cut(s)	289, 377
HinfI	GANTC	1 cut(s)	197
HphI	GGTGA	4 cut(s)	61, 130, 191, 568
Hpy166II	GTNNAC	4 cut(s)	79, 289, 334, 377
Hpy188I	TCNGA	2 cut(s)	501, 528
Hpy188III	TCNNGA	2 cut(s)	104, 269
Hpy8I	GTNNAC	4 cut(s)	79, 289, 334, 377
Hpy99I	CGWCG	1 cut(s)	389
HpyAV	CCTTC	3 cut(s)	302, 418, 618
HpyCH4IV	ACGT	1 cut(s)	379
HpyCH4V	TGCA	5 cut(s)	236, 392, 404, 581, 628
HpyF10VI	GCNNNNNNNGC	2 cut(s)	154, 233
HpyF3I	CTNAG	1 cut(s)	500
HpySE526I	ACGT	1 cut(s)	379
Hsp92II	CATG	2 cut(s)	408, 585
Kzo9I	GATC	3 cut(s)	100, 207, 496
LmnI	GCTCC	1 cut(s)	351
Lsp1109I	GCAGC	4 cut(s)	44, 236, 376, 640
MabI	ACCWGGT	1 cut(s)	327
MaeI	CTAG	3 cut(s)	201, 636, 646
MaeII	ACGT	1 cut(s)	379
MaeIII	GTNAC	3 cut(s)	179, 556, 638
MalI	GATC	3 cut(s)	102, 209, 498
MboI	GATC	3 cut(s)	100, 207, 496
MboII	GAAGA	4 cut(s)	185, 500, 580, 628
MhlI	GDGCHC	2 cut(s)	120, 348
MluCI	AATT	1 cut(s)	87
MmeI	TCCRAC	1 cut(s)	624
MnlI	CCTC	6 cut(s)	100, 265, 403, 436, 558, 583
Mph1103I	ATGCAT	2 cut(s)	406, 583
MseI	TTAA	1 cut(s)	395
MslI	CAYNNNNRTG	1 cut(s)	341
MspR9I	CCNGG	2 cut(s)	329, 513
Mva1269I	GAATGC	1 cut(s)	583
MvaI	CCWGG	2 cut(s)	329, 513
MwoI	GCNNNNNNNGC	2 cut(s)	154, 233
NdeII	GATC	3 cut(s)	100, 207, 496
NlaIII	CATG	2 cut(s)	408, 585
NlaIV	GGNNCC	1 cut(s)	247
NmuCI	GTSAC	3 cut(s)	179, 556, 638
NsiI	ATGCAT	2 cut(s)	406, 583
NspI	RCATGY	1 cut(s)	408
OliI	CACNNNNGTG	1 cut(s)	341
PceI	AGGCCT	1 cut(s)	607
PctI	GAATGC	1 cut(s)	583
PfeI	GAWTC	1 cut(s)	197
PflMI	CCANNNNNTGG	1 cut(s)	298
PkrI	GCNGC	4 cut(s)	34, 226, 391, 630
PshBI	ATTAAT	1 cut(s)	395
Psp6I	CCWGG	2 cut(s)	327, 511
PspGI	CCWGG	2 cut(s)	327, 511
PspN4I	GGNNCC	1 cut(s)	247
PspPI	GGNCC	2 cut(s)	20, 245
PstNI	CAGNNNCTG	1 cut(s)	298
RsaI	GTAC	1 cut(s)	218
RsaNI	GTAC	1 cut(s)	217
RseI	CAYNNNNRTG	1 cut(s)	341
SaqAI	TTAA	1 cut(s)	395
SatI	GCNGC	4 cut(s)	33, 225, 390, 629
Sau3AI	GATC	3 cut(s)	100, 207, 496
Sau96I	GGNCC	2 cut(s)	20, 245
ScrFI	CCNGG	2 cut(s)	329, 513
SduI	GDGCHC	2 cut(s)	120, 348
SetI	ASST	9 cut(s)	218, 255, 330, 354, 382, 429, 524, 541, 633
SexAI	ACCWGGT	1 cut(s)	327
SinI	GGWCC	2 cut(s)	20, 245
SmiMI	CAYNNNNRTG	1 cut(s)	341
SmlI	CTYRAG	1 cut(s)	104
SmoI	CTYRAG	1 cut(s)	104
Sse9I	AATT	1 cut(s)	87
SseBI	AGGCCT	1 cut(s)	607
SspMI	CTAG	3 cut(s)	201, 636, 646
StuI	AGGCCT	1 cut(s)	607
StyD4I	CCNGG	2 cut(s)	327, 511
TaiI	ACGT	1 cut(s)	382
TasI	AATT	1 cut(s)	87
TfiI	GAWTC	1 cut(s)	197
Tru1I	TTAA	1 cut(s)	395
Tru9I	TTAA	1 cut(s)	395
TscAI	CASTG	1 cut(s)	299
TseFI	GTSAC	3 cut(s)	179, 556, 638
TseI	GCWGC	4 cut(s)	32, 224, 389, 628
Tsp45I	GTSAC	3 cut(s)	179, 556, 638
TspDTI	ATGAA	1 cut(s)	549
TspGWI	ACGGA	1 cut(s)	382
TspRI	CASTG	1 cut(s)	299
Van91I	CCANNNNNTGG	1 cut(s)	298
VpaK11BI	GGWCC	2 cut(s)	20, 245
VspI	ATTAAT	1 cut(s)	395
XceI	RCATGY	1 cut(s)	408
XspI	CTAG	3 cut(s)	201, 636, 646
Zsp2I	ATGCAT	2 cut(s)	406, 583

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.