Prupe.1G121100_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0018526
Physical Location & Seq
Forward (+)
9501157 .. 9502201
1045 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G121100.1

Sequence Viewer

Length: 585 bp
ATGACGAGTCATCAAGTGAAAAATCAGGTCAGAGACAGTTTTGAGCAAGGCTTCGACAGCCTGCACAGTGTTGCTGATGTCGTGACTTTCCCTCTGGCCCCTCTGGACGGTGCCATCCATGGCACTTCCCGAGGGGTCCTCAACTGGCTCACAGATACCCATCCTGAAGATAGATCCAACTCCAAGCAAAAGGGTCTTCCCAACGGTGGCGGCTACGGCGGTGGACAGGGCAGCGGTTACGGCGGCGGAAACGGCAGAAATGGGGGAGGATATCCTCTGGGTATTAATCCCAACCGCAAACTTGTCACTGTCAAAGGGAATCAAATTAGTGACAACAACGCCGACGCTCAGGCATTTAACAACTTCGCCAACAAGTATAACGGGGGCTACAACGGTCATAACTACCACGACAACGGGCAGGGATTTAACAACTCCGGCAACCGGAATAACGGGGGCTACAATGGTCAGAACCACGACGATGATGACGATGAAGACGACGCTATTTTCGGATTTGAGGATAATAAAATCAGTGGGAACAAGGGTAAGGTGAAAGGCTTTAACGACTTTGGTAACCAGTACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

195

Amino Acids

20.75

Weight (kDa)

5.54

Isoelectric Point (pI)

15.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 110
AciI CCGC 6 cut(s) 210, 219, 234, 243, 246, 295
AclWI GGATC 1 cut(s) 168
AcuI CTGAAG 1 cut(s) 186
AfaI GTAC 1 cut(s) 578
AfiI CCNNNNNNNGG 3 cut(s) 107, 206, 441
Alw26I GTCTC 1 cut(s) 27
AlwI GGATC 1 cut(s) 168
Ama87I CYCGRG 1 cut(s) 129
AoxI GGCC 1 cut(s) 96
ApeKI GCWGC 1 cut(s) 231
ArsI GACNNNNNNTTYG 2 cut(s) 488, 520
AseI ATTAAT 1 cut(s) 285
AspS9I GGNCC 2 cut(s) 97, 136
AsuHPI GGTGA 1 cut(s) 559
AvaI CYCGRG 1 cut(s) 129
AvaII GGWCC 1 cut(s) 136
BanI GGYRCC 1 cut(s) 110
BbsI GAAGAC 2 cut(s) 188, 498
BbvI GCAGC 1 cut(s) 243
BccI CCATC 2 cut(s) 122, 168
BceAI ACGGC 3 cut(s) 232, 256, 268
BcoDI GTCTC 1 cut(s) 27
BisI GCNGC 3 cut(s) 211, 232, 244
BlsI GCNGC 3 cut(s) 212, 233, 245
BmcAI AGTACT 1 cut(s) 578
Bme18I GGWCC 1 cut(s) 136
BmeT110I CYCGRG 1 cut(s) 129
BmgT120I GGNCC 2 cut(s) 97, 136
BmiI GGNNCC 3 cut(s) 99, 112, 137
BpiI GAAGAC 2 cut(s) 188, 498
BplI GAGNNNNNCTC 2 cut(s) 123, 155
Bpu10I CCTNAGC 1 cut(s) 348
BsaBI GATNNNNATC 1 cut(s) 159
BsaJI CCNNGG 2 cut(s) 118, 130
BsaWI WCCGGW 1 cut(s) 441
Bsc4I CCNNNNNNNGG 3 cut(s) 107, 206, 441
Bse1I ACTGG 2 cut(s) 149, 574
Bse8I GATNNNNATC 1 cut(s) 159
BseDI CCNNGG 2 cut(s) 118, 130
BseGI GGATG 2 cut(s) 114, 160
BseJI GATNNNNATC 1 cut(s) 159
BseLI CCNNNNNNNGG 3 cut(s) 107, 206, 441
BseMII CTCAG 1 cut(s) 362
BseNI ACTGG 2 cut(s) 149, 574
BseXI GCAGC 1 cut(s) 243
BsgI GTGCAG 1 cut(s) 47
BshFI GGCC 1 cut(s) 98
BshNI GGYRCC 1 cut(s) 110
BsiHKCI CYCGRG 1 cut(s) 129
BsiSI CCGG 2 cut(s) 435, 442
BslI CCNNNNNNNGG 3 cut(s) 107, 206, 441
BsmAI GTCTC 1 cut(s) 27
BsnI GGCC 1 cut(s) 98
BsoBI CYCGRG 1 cut(s) 129
Bsp143I GATC 1 cut(s) 173
Bsp19I CCATGG 1 cut(s) 118
BspACI CCGC 6 cut(s) 210, 219, 234, 243, 246, 295
BspANI GGCC 1 cut(s) 98
BspCNI CTCAG 1 cut(s) 361
BspLI GGNNCC 3 cut(s) 99, 112, 137
BspPI GGATC 1 cut(s) 168
BspT107I GGYRCC 1 cut(s) 110
BsrI ACTGG 2 cut(s) 149, 574
BssECI CCNNGG 2 cut(s) 118, 130
BssMI GATC 1 cut(s) 173
BssT1I CCWWGG 1 cut(s) 118
Bst4CI ACNGT 6 cut(s) 38, 68, 110, 206, 310, 395
BstC8I GCNNGC 1 cut(s) 62
BstDEI CTNAG 1 cut(s) 348
BstDSI CCRYGG 1 cut(s) 118
BstEII GGTNACC 1 cut(s) 569
BstF5I GGATG 2 cut(s) 114, 160
BstKTI GATC 1 cut(s) 176
BstMAI GTCTC 1 cut(s) 27
BstMBI GATC 1 cut(s) 173
BstMWI GCNNNNNNNGC 4 cut(s) 57, 216, 240, 252
BstPI GGTNACC 1 cut(s) 569
BstV1I GCAGC 1 cut(s) 243
BstV2I GAAGAC 2 cut(s) 188, 498
BstX2I RGATCY 1 cut(s) 173
BstYI RGATCY 1 cut(s) 173
BsuRI GGCC 1 cut(s) 98
BtgI CCRYGG 1 cut(s) 118
BtsCI GGATG 2 cut(s) 114, 160
BtsIMutI CAGTG 3 cut(s) 73, 306, 535
Cac8I GCNNGC 1 cut(s) 62
Cfr13I GGNCC 2 cut(s) 97, 136
CseI GACGC 2 cut(s) 353, 506
Csp6I GTAC 1 cut(s) 577
CviAII CATG 1 cut(s) 119
CviJI RGCY 8 cut(s) 51, 60, 98, 148, 213, 387, 456, 555
CviKI_1 RGCY 8 cut(s) 51, 60, 98, 148, 213, 387, 456, 555
CviQI GTAC 1 cut(s) 577
DdeI CTNAG 1 cut(s) 348
DpnI GATC 1 cut(s) 175
DpnII GATC 1 cut(s) 173
EciI GGCGGA 1 cut(s) 261
Eco130I CCWWGG 1 cut(s) 118
Eco32I GATATC 1 cut(s) 272
Eco47I GGWCC 1 cut(s) 136
Eco57I CTGAAG 1 cut(s) 186
Eco88I CYCGRG 1 cut(s) 129
Eco91I GGTNACC 1 cut(s) 569
EcoO109I RGGNCCY 1 cut(s) 136
EcoO65I GGTNACC 1 cut(s) 569
EcoRV GATATC 1 cut(s) 272
EcoT14I CCWWGG 1 cut(s) 118
ErhI CCWWGG 1 cut(s) 118
FaeI CATG 1 cut(s) 122
FaiI YATR 3 cut(s) 120, 378, 399
FatI CATG 1 cut(s) 118
Fnu4HI GCNGC 3 cut(s) 211, 232, 244
FokI GGATG 2 cut(s) 101, 147
Fsp4HI GCNGC 3 cut(s) 211, 232, 244
GluI GCNGC 3 cut(s) 211, 232, 244
HaeIII GGCC 1 cut(s) 98
HapII CCGG 2 cut(s) 435, 442
HgaI GACGC 2 cut(s) 353, 506
Hin1II CATG 1 cut(s) 122
HinfI GANTC 2 cut(s) 7, 319
HpaII CCGG 2 cut(s) 435, 442
HphI GGTGA 1 cut(s) 559
Hpy166II GTNNAC 1 cut(s) 224
Hpy188I TCNGA 4 cut(s) 32, 468, 509, 584
Hpy188III TCNNGA 4 cut(s) 82, 104, 129, 164
Hpy8I GTNNAC 1 cut(s) 224
Hpy99I CGWCG 3 cut(s) 347, 479, 500
HpyCH4III ACNGT 6 cut(s) 38, 68, 110, 206, 310, 395
HpyCH4V TGCA 1 cut(s) 64
HpyF10VI GCNNNNNNNGC 4 cut(s) 57, 216, 240, 252
HpyF3I CTNAG 1 cut(s) 348
Hsp92II CATG 1 cut(s) 122
Kzo9I GATC 1 cut(s) 173
Lsp1109I GCAGC 1 cut(s) 243
MaeIII GTNAC 5 cut(s) 82, 236, 304, 329, 569
MalI GATC 1 cut(s) 175
MboI GATC 1 cut(s) 173
MboII GAAGA 3 cut(s) 179, 188, 503
MflI RGATCY 1 cut(s) 173
MluCI AATT 1 cut(s) 324
MlyI GAGTC 1 cut(s) 16
MmeI TCCRAC 1 cut(s) 201
MnlI CCTC 7 cut(s) 102, 111, 125, 149, 260, 285, 508
MseI TTAA 4 cut(s) 285, 357, 426, 558
MslI CAYNNNNRTG 1 cut(s) 477
MspA1I CMGCKG 1 cut(s) 234
MspI CCGG 2 cut(s) 435, 442
MwoI GCNNNNNNNGC 4 cut(s) 57, 216, 240, 252
NcoI CCATGG 1 cut(s) 118
NdeII GATC 1 cut(s) 173
NlaIII CATG 1 cut(s) 122
NlaIV GGNNCC 3 cut(s) 99, 112, 137
NmuCI GTSAC 3 cut(s) 82, 304, 329
PcsI WCGNNNNNNNCGW 2 cut(s) 483, 492
PfeI GAWTC 1 cut(s) 319
PkrI GCNGC 3 cut(s) 212, 233, 245
PleI GAGTC 1 cut(s) 15
PpsI GAGTC 1 cut(s) 15
PpuMI RGGWCCY 1 cut(s) 136
PshBI ATTAAT 1 cut(s) 285
Psp5II RGGWCCY 1 cut(s) 136
PspEI GGTNACC 1 cut(s) 569
PspN4I GGNNCC 3 cut(s) 99, 112, 137
PspPI GGNCC 2 cut(s) 97, 136
PspPPI RGGWCCY 1 cut(s) 136
PsuI RGATCY 1 cut(s) 173
RsaI GTAC 1 cut(s) 578
RsaNI GTAC 1 cut(s) 577
RseI CAYNNNNRTG 1 cut(s) 477
SaqAI TTAA 4 cut(s) 285, 357, 426, 558
SatI GCNGC 3 cut(s) 211, 232, 244
Sau3AI GATC 1 cut(s) 173
Sau96I GGNCC 2 cut(s) 97, 136
ScaI AGTACT 1 cut(s) 578
SchI GAGTC 1 cut(s) 16
SetI ASST 2 cut(s) 30, 549
SinI GGWCC 1 cut(s) 136
SmiMI CAYNNNNRTG 1 cut(s) 477
Sse9I AATT 1 cut(s) 324
SsiI CCGC 6 cut(s) 210, 219, 234, 243, 246, 295
StyI CCWWGG 1 cut(s) 118
TaaI ACNGT 6 cut(s) 38, 68, 110, 206, 310, 395
TaqI TCGA 1 cut(s) 54
TasI AATT 1 cut(s) 324
TatI WGTACW 1 cut(s) 576
TauI GCSGC 2 cut(s) 213, 246
TfiI GAWTC 1 cut(s) 319
Tru1I TTAA 4 cut(s) 285, 357, 426, 558
Tru9I TTAA 4 cut(s) 285, 357, 426, 558
TscAI CASTG 3 cut(s) 73, 313, 535
TseFI GTSAC 3 cut(s) 82, 304, 329
TseI GCWGC 1 cut(s) 231
Tsp45I GTSAC 3 cut(s) 82, 304, 329
TspDTI ATGAA 1 cut(s) 504
TspRI CASTG 3 cut(s) 73, 313, 535
VpaK11BI GGWCC 1 cut(s) 136
VspI ATTAAT 1 cut(s) 285
ZrmI AGTACT 1 cut(s) 578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.