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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 192 |
| AciI | CCGC | 1 cut(s) | 192 |
| AclWI | GGATC | 1 cut(s) | 219 |
| AfaI | GTAC | 1 cut(s) | 171 |
| AflIII | ACRYGT | 1 cut(s) | 60 |
| AgsI | TTSAA | 1 cut(s) | 166 |
| AjiI | CACGTC | 1 cut(s) | 63 |
| AluBI | AGCT | 1 cut(s) | 92 |
| AluI | AGCT | 1 cut(s) | 92 |
| AlwI | GGATC | 1 cut(s) | 219 |
| AoxI | GGCC | 1 cut(s) | 202 |
| AspS9I | GGNCC | 1 cut(s) | 202 |
| AxyI | CCTNAGG | 1 cut(s) | 234 |
| BmcAI | AGTACT | 1 cut(s) | 171 |
| BmgBI | CACGTC | 1 cut(s) | 63 |
| BmgT120I | GGNCC | 1 cut(s) | 202 |
| BmiI | GGNNCC | 1 cut(s) | 10 |
| BmsI | GCATC | 1 cut(s) | 124 |
| Bse1I | ACTGG | 1 cut(s) | 205 |
| Bse21I | CCTNAGG | 1 cut(s) | 234 |
| BseGI | GGATG | 1 cut(s) | 139 |
| BseNI | ACTGG | 1 cut(s) | 205 |
| Bsh1236I | CGCG | 1 cut(s) | 192 |
| BshFI | GGCC | 1 cut(s) | 204 |
| BsnI | GGCC | 1 cut(s) | 204 |
| Bsp143I | GATC | 1 cut(s) | 211 |
| BspACI | CCGC | 1 cut(s) | 192 |
| BspANI | GGCC | 1 cut(s) | 204 |
| BspFNI | CGCG | 1 cut(s) | 192 |
| BspLI | GGNNCC | 1 cut(s) | 10 |
| BspPI | GGATC | 1 cut(s) | 219 |
| BsrI | ACTGG | 1 cut(s) | 205 |
| BssMI | GATC | 1 cut(s) | 211 |
| BstDEI | CTNAG | 1 cut(s) | 234 |
| BstF5I | GGATG | 1 cut(s) | 139 |
| BstFNI | CGCG | 1 cut(s) | 192 |
| BstKTI | GATC | 1 cut(s) | 214 |
| BstMBI | GATC | 1 cut(s) | 211 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 89, 108 |
| BstUI | CGCG | 1 cut(s) | 192 |
| Bsu36I | CCTNAGG | 1 cut(s) | 234 |
| BsuRI | GGCC | 1 cut(s) | 204 |
| BtrI | CACGTC | 1 cut(s) | 63 |
| BtsCI | GGATG | 1 cut(s) | 139 |
| Cfr13I | GGNCC | 1 cut(s) | 202 |
| Csp6I | GTAC | 1 cut(s) | 170 |
| CviAII | CATG | 1 cut(s) | 240 |
| CviJI | RGCY | 3 cut(s) | 5, 92, 204 |
| CviKI_1 | RGCY | 3 cut(s) | 5, 92, 204 |
| CviQI | GTAC | 1 cut(s) | 170 |
| DdeI | CTNAG | 1 cut(s) | 234 |
| DpnI | GATC | 1 cut(s) | 213 |
| DpnII | GATC | 1 cut(s) | 211 |
| Eco81I | CCTNAGG | 1 cut(s) | 234 |
| FaeI | CATG | 1 cut(s) | 243 |
| FaiI | YATR | 1 cut(s) | 241 |
| FatI | CATG | 1 cut(s) | 239 |
| FokI | GGATG | 1 cut(s) | 146 |
| HaeIII | GGCC | 1 cut(s) | 204 |
| Hin1II | CATG | 1 cut(s) | 243 |
| Hpy188I | TCNGA | 1 cut(s) | 211 |
| Hpy188III | TCNNGA | 1 cut(s) | 66 |
| Hpy99I | CGWCG | 1 cut(s) | 67 |
| HpyCH4IV | ACGT | 1 cut(s) | 62 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 89, 108 |
| HpyF3I | CTNAG | 1 cut(s) | 234 |
| HpySE526I | ACGT | 1 cut(s) | 62 |
| Hsp92II | CATG | 1 cut(s) | 243 |
| Kzo9I | GATC | 1 cut(s) | 211 |
| LpnPI | CCDG | 2 cut(s) | 218, 221 |
| LweI | GCATC | 1 cut(s) | 124 |
| MaeII | ACGT | 1 cut(s) | 62 |
| MalI | GATC | 1 cut(s) | 213 |
| MboI | GATC | 1 cut(s) | 211 |
| MluCI | AATT | 1 cut(s) | 24 |
| MmeI | TCCRAC | 1 cut(s) | 189 |
| MnlI | CCTC | 3 cut(s) | 118, 124, 243 |
| MseI | TTAA | 3 cut(s) | 21, 27, 71 |
| MvnI | CGCG | 1 cut(s) | 192 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 89, 108 |
| NdeII | GATC | 1 cut(s) | 211 |
| NlaIII | CATG | 1 cut(s) | 243 |
| NlaIV | GGNNCC | 1 cut(s) | 10 |
| PspN4I | GGNNCC | 1 cut(s) | 10 |
| PspPI | GGNCC | 1 cut(s) | 202 |
| RsaI | GTAC | 1 cut(s) | 171 |
| RsaNI | GTAC | 1 cut(s) | 170 |
| SaqAI | TTAA | 3 cut(s) | 21, 27, 71 |
| Sau3AI | GATC | 1 cut(s) | 211 |
| Sau96I | GGNCC | 1 cut(s) | 202 |
| ScaI | AGTACT | 1 cut(s) | 171 |
| SetI | ASST | 4 cut(s) | 65, 94, 129, 235 |
| SfaNI | GCATC | 1 cut(s) | 124 |
| Sse9I | AATT | 1 cut(s) | 24 |
| SsiI | CCGC | 1 cut(s) | 192 |
| TaiI | ACGT | 1 cut(s) | 65 |
| TaqI | TCGA | 1 cut(s) | 123 |
| TaqII | GACCGA | 1 cut(s) | 191 |
| TasI | AATT | 1 cut(s) | 24 |
| TatI | WGTACW | 1 cut(s) | 169 |
| Tru1I | TTAA | 3 cut(s) | 21, 27, 71 |
| Tru9I | TTAA | 3 cut(s) | 21, 27, 71 |
| ZrmI | AGTACT | 1 cut(s) | 171 |