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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 58, 102 |
| AfaI | GTAC | 1 cut(s) | 270 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 27 |
| AgeI | ACCGGT | 1 cut(s) | 239 |
| AgsI | TTSAA | 1 cut(s) | 173 |
| AluBI | AGCT | 2 cut(s) | 162, 176 |
| AluI | AGCT | 2 cut(s) | 162, 176 |
| Ama87I | CYCGRG | 1 cut(s) | 154 |
| AoxI | GGCC | 1 cut(s) | 147 |
| ApeKI | GCWGC | 2 cut(s) | 159, 301 |
| AsiGI | ACCGGT | 1 cut(s) | 239 |
| AspS9I | GGNCC | 1 cut(s) | 147 |
| AsuC2I | CCSGG | 1 cut(s) | 283 |
| AsuHPI | GGTGA | 1 cut(s) | 254 |
| AvaI | CYCGRG | 1 cut(s) | 154 |
| BbvI | GCAGC | 2 cut(s) | 171, 288 |
| BccI | CCATC | 1 cut(s) | 106 |
| BcnI | CCSGG | 1 cut(s) | 283 |
| BisI | GCNGC | 3 cut(s) | 103, 160, 302 |
| BlsI | GCNGC | 3 cut(s) | 104, 161, 303 |
| BmcAI | AGTACT | 1 cut(s) | 270 |
| Bme1390I | CCNGG | 1 cut(s) | 283 |
| BmeT110I | CYCGRG | 1 cut(s) | 154 |
| BmgT120I | GGNCC | 1 cut(s) | 147 |
| BmiI | GGNNCC | 1 cut(s) | 148 |
| BmrFI | CCNGG | 1 cut(s) | 283 |
| BpuEI | CTTGAG | 1 cut(s) | 10 |
| BpuMI | CCSGG | 1 cut(s) | 283 |
| BsaWI | WCCGGW | 1 cut(s) | 239 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 27 |
| Bse118I | RCCGGY | 1 cut(s) | 239 |
| BseGI | GGATG | 1 cut(s) | 278 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 27 |
| BseMII | CTCAG | 1 cut(s) | 168 |
| BseXI | GCAGC | 2 cut(s) | 171, 288 |
| BshFI | GGCC | 1 cut(s) | 149 |
| BshTI | ACCGGT | 1 cut(s) | 239 |
| BsiHKCI | CYCGRG | 1 cut(s) | 154 |
| BsiSI | CCGG | 2 cut(s) | 240, 282 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 27 |
| BsnI | GGCC | 1 cut(s) | 149 |
| BsoBI | CYCGRG | 1 cut(s) | 154 |
| BspACI | CCGC | 2 cut(s) | 58, 102 |
| BspANI | GGCC | 1 cut(s) | 149 |
| BspCNI | CTCAG | 1 cut(s) | 169 |
| BspLI | GGNNCC | 1 cut(s) | 148 |
| BsrFI | RCCGGY | 1 cut(s) | 239 |
| BssAI | RCCGGY | 1 cut(s) | 239 |
| BstDEI | CTNAG | 1 cut(s) | 177 |
| BstF5I | GGATG | 1 cut(s) | 278 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 47, 99 |
| BstSCI | CCNGG | 1 cut(s) | 281 |
| BstV1I | GCAGC | 2 cut(s) | 171, 288 |
| BsuRI | GGCC | 1 cut(s) | 149 |
| BtsCI | GGATG | 1 cut(s) | 278 |
| Cfr10I | RCCGGY | 1 cut(s) | 239 |
| Cfr13I | GGNCC | 1 cut(s) | 147 |
| Csp6I | GTAC | 1 cut(s) | 269 |
| CspAI | ACCGGT | 1 cut(s) | 239 |
| CviAII | CATG | 1 cut(s) | 190 |
| CviJI | RGCY | 4 cut(s) | 149, 162, 176, 301 |
| CviKI_1 | RGCY | 4 cut(s) | 149, 162, 176, 301 |
| CviQI | GTAC | 1 cut(s) | 269 |
| DdeI | CTNAG | 1 cut(s) | 177 |
| Eco32I | GATATC | 1 cut(s) | 88 |
| Eco88I | CYCGRG | 1 cut(s) | 154 |
| EcoRV | GATATC | 1 cut(s) | 88 |
| FaeI | CATG | 1 cut(s) | 193 |
| FaiI | YATR | 2 cut(s) | 39, 191 |
| FatI | CATG | 1 cut(s) | 189 |
| Fnu4HI | GCNGC | 3 cut(s) | 103, 160, 302 |
| FokI | GGATG | 1 cut(s) | 265 |
| Fsp4HI | GCNGC | 3 cut(s) | 103, 160, 302 |
| GluI | GCNGC | 3 cut(s) | 103, 160, 302 |
| HaeIII | GGCC | 1 cut(s) | 149 |
| HapII | CCGG | 2 cut(s) | 240, 282 |
| Hin1II | CATG | 1 cut(s) | 193 |
| HinfI | GANTC | 2 cut(s) | 7, 152 |
| HpaII | CCGG | 2 cut(s) | 240, 282 |
| HphI | GGTGA | 1 cut(s) | 254 |
| Hpy188III | TCNNGA | 2 cut(s) | 11, 274 |
| HpyAV | CCTTC | 2 cut(s) | 187, 291 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 47, 99 |
| HpyF3I | CTNAG | 1 cut(s) | 177 |
| Hsp92II | CATG | 1 cut(s) | 193 |
| LpnPI | CCDG | 3 cut(s) | 27, 253, 295 |
| Lsp1109I | GCAGC | 2 cut(s) | 171, 288 |
| MaeIII | GTNAC | 1 cut(s) | 8 |
| MboII | GAAGA | 3 cut(s) | 300, 303, 306 |
| MluCI | AATT | 3 cut(s) | 72, 168, 307 |
| MlyI | GAGTC | 2 cut(s) | 16, 146 |
| MnlI | CCTC | 1 cut(s) | 221 |
| MseI | TTAA | 3 cut(s) | 69, 75, 186 |
| MspA1I | CMGCKG | 2 cut(s) | 102, 162 |
| MspI | CCGG | 2 cut(s) | 240, 282 |
| MspR9I | CCNGG | 1 cut(s) | 283 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 47, 99 |
| NciI | CCSGG | 1 cut(s) | 283 |
| NlaIII | CATG | 1 cut(s) | 193 |
| NlaIV | GGNNCC | 1 cut(s) | 148 |
| NmuCI | GTSAC | 1 cut(s) | 8 |
| PfoI | TCCNGGA | 1 cut(s) | 281 |
| PinAI | ACCGGT | 1 cut(s) | 239 |
| PkrI | GCNGC | 3 cut(s) | 104, 161, 303 |
| PleI | GAGTC | 2 cut(s) | 15, 146 |
| PpsI | GAGTC | 2 cut(s) | 15, 146 |
| PspN4I | GGNNCC | 1 cut(s) | 148 |
| PspPI | GGNCC | 1 cut(s) | 147 |
| PvuII | CAGCTG | 1 cut(s) | 162 |
| RsaI | GTAC | 1 cut(s) | 270 |
| RsaNI | GTAC | 1 cut(s) | 269 |
| SaqAI | TTAA | 3 cut(s) | 69, 75, 186 |
| SatI | GCNGC | 3 cut(s) | 103, 160, 302 |
| Sau96I | GGNCC | 1 cut(s) | 147 |
| ScaI | AGTACT | 1 cut(s) | 270 |
| SchI | GAGTC | 2 cut(s) | 16, 146 |
| ScrFI | CCNGG | 1 cut(s) | 283 |
| SetI | ASST | 3 cut(s) | 164, 178, 213 |
| SgrAI | CRCCGGYG | 1 cut(s) | 239 |
| SmlI | CTYRAG | 1 cut(s) | 25 |
| SmoI | CTYRAG | 1 cut(s) | 25 |
| Sse9I | AATT | 3 cut(s) | 72, 168, 307 |
| SsiI | CCGC | 2 cut(s) | 58, 102 |
| SspI | AATATT | 1 cut(s) | 184 |
| StyD4I | CCNGG | 1 cut(s) | 281 |
| TasI | AATT | 3 cut(s) | 72, 168, 307 |
| TatI | WGTACW | 1 cut(s) | 268 |
| TauI | GCSGC | 1 cut(s) | 105 |
| Tru1I | TTAA | 3 cut(s) | 69, 75, 186 |
| Tru9I | TTAA | 3 cut(s) | 69, 75, 186 |
| TseFI | GTSAC | 1 cut(s) | 8 |
| TseI | GCWGC | 2 cut(s) | 159, 301 |
| Tsp45I | GTSAC | 1 cut(s) | 8 |
| TspDTI | ATGAA | 2 cut(s) | 30, 206 |
| ZrmI | AGTACT | 1 cut(s) | 270 |