Prupe.1G301100_v2.0.a1

HD domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0009302
Physical Location & Seq
Forward (+)
29620820 .. 29624638
3819 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G301100.2

Sequence Viewer

Length: 879 bp
ATGGCGCTTAATTCACCGACTCGCTGCGCGGCTCCACTGACCCGCTCTGTCCCTCCTCGTTCGCCTCTCGCTTTGTTCAATCGGACCTTGCCCAGACCCGCATTTTCAACCGAATCCCCGACCGCCAGGCTCGTCTCCGTTCGTTGCCAGACTCTCACTCCGGACGGCGTCCCGTCTAAGCGGCCTGTGGGTTTGGACGAGTCGGAAGCTTCGATTGGGTCGTCGGGCTCTTCGTCCACGTCCTCCGCTATTGATTTTCTCACACTGTGCCATAGTCTCAAGACCACAAAAAGAAAGGGCTGGATCAATCATGGAATAAAGGGTCCTGAGTCCATTGCTGACCATATGTACCGCATGGCTTTGATGTCTTTGATTGCTGGTGACGTTCCTGGCTTGAACAGAGAAAGGTGTATCAAGATAGCAATTGTCCATGACATTGCAGAAGCTATTGTTGGAGATATAACACCAGCTGATGGCATACCCAAAGCAGAAAAGAGCAGAAGGGAGCAAGCAGCTTTGAATGAAATGTGCATAGTTCTTGGTGGAGGGATGAGGGCTGAAGAGATCAAAGAACTTTGGGCAGAGTATGAAAATAATTCGTCCTTAGAGGCTAATCTTGTGAAAGATTTTGATAAAGTTGAAATGATTCTGCAAGCATTGGAATATGAAATAGGTAAAGTGTTTCATTCTTTTGCTGTAACTTTGTTAGCTTTCTTATCTTTCTCTTCTGATATTTTTACCCTCGTAACAGAACATGGGAAGGTGCTGGATGAGTTTTTCATTTCTACCGCAGGAAAATTTCAAACTGAACTAGGAAAGAGTTGGGCAGCTGAAATCATTTCTAGAAGAAATTCACGGGTTGACAAGAGTCATAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HD_3

Protein Analysis

293

Amino Acids

31.92

Weight (kDa)

6.67

Isoelectric Point (pI)

42.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 473
AccBSI CCGCTC 1 cut(s) 45
AccII CGCG 1 cut(s) 29
AccIII TCCGGA 1 cut(s) 160
AciI CCGC 8 cut(s) 29, 43, 99, 123, 181, 246, 352, 789
AclWI GGATC 1 cut(s) 311
AcsI RAATTY 2 cut(s) 797, 850
AcuI CTGAAG 1 cut(s) 579
AcyI GRCGYC 1 cut(s) 168
AdeI CACNNNGTG 1 cut(s) 267
AfaI GTAC 1 cut(s) 350
AfiI CCNNNNNNNGG 1 cut(s) 473
AgsI TTSAA 6 cut(s) 79, 108, 397, 520, 641, 803
AjiI CACGTC 1 cut(s) 240
AjnI CCWGG 2 cut(s) 125, 388
AjuI GAANNNNNNNTTGG 4 cut(s) 198, 230, 435, 467
AluBI AGCT 6 cut(s) 209, 446, 470, 515, 710, 830
AluI AGCT 6 cut(s) 209, 446, 470, 515, 710, 830
Alw26I GTCTC 2 cut(s) 139, 281
AlwI GGATC 1 cut(s) 311
Aor13HI TCCGGA 1 cut(s) 160
AoxI GGCC 1 cut(s) 182
ApeKI GCWGC 3 cut(s) 24, 512, 827
ApoI RAATTY 2 cut(s) 797, 850
Asp700I GAANNNNTTC 2 cut(s) 645, 850
AspLEI GCGC 2 cut(s) 7, 29
AspS9I GGNCC 2 cut(s) 84, 323
AsuHPI GGTGA 2 cut(s) 6, 392
AvaII GGWCC 2 cut(s) 84, 323
BanII GRGCYC 1 cut(s) 230
BbvI GCAGC 3 cut(s) 11, 524, 839
BccI CCATC 1 cut(s) 467
BceAI ACGGC 1 cut(s) 181
BciT130I CCWGG 2 cut(s) 127, 390
BcoDI GTCTC 2 cut(s) 139, 281
BfaI CTAG 2 cut(s) 812, 843
BfoI RGCGCY 1 cut(s) 8
BisI GCNGC 5 cut(s) 25, 30, 182, 513, 828
BlsI GCNGC 5 cut(s) 26, 31, 183, 514, 829
Bme1390I CCNGG 2 cut(s) 127, 390
Bme18I GGWCC 2 cut(s) 84, 323
BmgBI CACGTC 1 cut(s) 240
BmgT120I GGNCC 2 cut(s) 84, 323
BmiI GGNNCC 2 cut(s) 33, 324
BmrFI CCNGG 2 cut(s) 127, 390
BoxI GACNNNNGTC 1 cut(s) 867
BpuEI CTTGAG 1 cut(s) 263
BsaHI GRCGYC 1 cut(s) 168
BsaWI WCCGGW 1 cut(s) 160
BsaXI ACNNNNNCTCC 2 cut(s) 142, 172
Bsc4I CCNNNNNNNGG 1 cut(s) 473
Bse3DI GCAATG 2 cut(s) 333, 435
BseAI TCCGGA 1 cut(s) 160
BseBI CCWGG 2 cut(s) 127, 390
BseGI GGATG 2 cut(s) 555, 775
BseLI CCNNNNNNNGG 1 cut(s) 473
BseMI GCAATG 2 cut(s) 333, 435
BseMII CTCAG 1 cut(s) 318
BseRI GAGGAG 1 cut(s) 45
BseXI GCAGC 3 cut(s) 11, 524, 839
Bsh1236I CGCG 1 cut(s) 29
Bsh1285I CGRYCG 1 cut(s) 123
BshFI GGCC 1 cut(s) 184
BsiEI CGRYCG 1 cut(s) 123
BsiSI CCGG 1 cut(s) 161
BslFI GGGAC 2 cut(s) 35, 155
BslI CCNNNNNNNGG 1 cut(s) 473
BsmAI GTCTC 2 cut(s) 139, 281
BsmBI CGTCTC 1 cut(s) 139
BsmFI GGGAC 2 cut(s) 35, 155
BsnI GGCC 1 cut(s) 184
Bsp1286I GDGCHC 1 cut(s) 230
Bsp13I TCCGGA 1 cut(s) 160
Bsp143I GATC 2 cut(s) 303, 564
BspACI CCGC 8 cut(s) 29, 43, 99, 123, 181, 246, 352, 789
BspANI GGCC 1 cut(s) 184
BspCNI CTCAG 1 cut(s) 319
BspEI TCCGGA 1 cut(s) 160
BspFNI CGCG 1 cut(s) 29
BspLI GGNNCC 2 cut(s) 33, 324
BspPI GGATC 1 cut(s) 311
BspQI GCTCTTC 1 cut(s) 235
BsrBI CCGCTC 1 cut(s) 45
BsrDI GCAATG 2 cut(s) 333, 435
BssMI GATC 2 cut(s) 303, 564
BssNI GRCGYC 1 cut(s) 168
Bst2UI CCWGG 2 cut(s) 127, 390
Bst4CI ACNGT 1 cut(s) 267
Bst6I CTCTTC 3 cut(s) 235, 555, 730
BstACI GRCGYC 1 cut(s) 168
BstC8I GCNNGC 2 cut(s) 510, 654
BstDEI CTNAG 3 cut(s) 177, 327, 604
BstF5I GGATG 2 cut(s) 555, 775
BstFNI CGCG 1 cut(s) 29
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 29
BstKTI GATC 2 cut(s) 306, 567
BstMAI GTCTC 2 cut(s) 139, 281
BstMBI GATC 2 cut(s) 303, 564
BstMCI CGRYCG 1 cut(s) 123
BstNI CCWGG 2 cut(s) 127, 390
BstPAI GACNNNNGTC 1 cut(s) 867
BstSCI CCNGG 2 cut(s) 125, 388
BstUI CGCG 1 cut(s) 29
BstV1I GCAGC 3 cut(s) 11, 524, 839
BsuRI GGCC 1 cut(s) 184
BtrI CACGTC 1 cut(s) 240
BtsCI GGATG 2 cut(s) 555, 775
BtsIMutI CAGTG 2 cut(s) 35, 263
Cac8I GCNNGC 2 cut(s) 510, 654
CfoI GCGC 2 cut(s) 7, 29
Cfr13I GGNCC 2 cut(s) 84, 323
CseI GACGC 1 cut(s) 157
Csp6I GTAC 1 cut(s) 349
CviAII CATG 4 cut(s) 311, 355, 431, 755
CviQI GTAC 1 cut(s) 349
DdeI CTNAG 3 cut(s) 177, 327, 604
DpnI GATC 2 cut(s) 305, 566
DpnII GATC 2 cut(s) 303, 564
DraIII CACNNNGTG 1 cut(s) 267
Eam1104I CTCTTC 3 cut(s) 235, 555, 730
EarI CTCTTC 3 cut(s) 235, 555, 730
Eco24I GRGCYC 1 cut(s) 230
Eco47I GGWCC 2 cut(s) 84, 323
Eco57I CTGAAG 1 cut(s) 579
EcoO109I RGGNCCY 1 cut(s) 323
EcoRII CCWGG 2 cut(s) 125, 388
EcoT38I GRGCYC 1 cut(s) 230
Esp3I CGTCTC 1 cut(s) 139
FaeI CATG 4 cut(s) 314, 358, 434, 758
FaqI GGGAC 2 cut(s) 35, 155
FatI CATG 4 cut(s) 310, 354, 430, 754
FauI CCCGC 2 cut(s) 50, 106
FauNDI CATATG 1 cut(s) 345
Fnu4HI GCNGC 5 cut(s) 25, 30, 182, 513, 828
FokI GGATG 2 cut(s) 562, 782
FriOI GRGCYC 1 cut(s) 230
Fsp4HI GCNGC 5 cut(s) 25, 30, 182, 513, 828
FspBI CTAG 2 cut(s) 812, 843
GlaI GCGC 2 cut(s) 6, 28
GluI GCNGC 5 cut(s) 25, 30, 182, 513, 828
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 184
HapII CCGG 1 cut(s) 161
HgaI GACGC 1 cut(s) 157
HhaI GCGC 2 cut(s) 7, 29
Hin1I GRCGYC 1 cut(s) 168
Hin1II CATG 4 cut(s) 314, 358, 434, 758
Hin6I GCGC 2 cut(s) 5, 27
HinP1I GCGC 2 cut(s) 5, 27
HincII GTYRAC 1 cut(s) 862
HindII GTYRAC 1 cut(s) 862
HindIII AAGCTT 1 cut(s) 207
HinfI GANTC 7 cut(s) 19, 113, 151, 200, 329, 646, 868
HpaII CCGG 1 cut(s) 161
HphI GGTGA 2 cut(s) 6, 392
Hpy166II GTNNAC 2 cut(s) 237, 862
Hpy188I TCNGA 3 cut(s) 84, 205, 730
Hpy188III TCNNGA 5 cut(s) 161, 280, 326, 415, 843
Hpy8I GTNNAC 2 cut(s) 237, 862
Hpy99I CGWCG 1 cut(s) 226
HpyAV CCTTC 2 cut(s) 495, 754
HpyCH4III ACNGT 1 cut(s) 267
HpyCH4IV ACGT 2 cut(s) 239, 384
HpyCH4V TGCA 3 cut(s) 440, 531, 652
HpyF3I CTNAG 3 cut(s) 177, 327, 604
HpySE526I ACGT 2 cut(s) 239, 384
Hsp92I GRCGYC 1 cut(s) 168
Hsp92II CATG 4 cut(s) 314, 358, 434, 758
HspAI GCGC 2 cut(s) 5, 27
Kpn2I TCCGGA 1 cut(s) 160
Kzo9I GATC 2 cut(s) 303, 564
LguI GCTCTTC 1 cut(s) 235
LmnI GCTCC 2 cut(s) 37, 505
Lsp1109I GCAGC 3 cut(s) 11, 524, 839
MaeI CTAG 2 cut(s) 812, 843
MaeII ACGT 2 cut(s) 239, 384
MaeIII GTNAC 3 cut(s) 380, 697, 745
MalI GATC 2 cut(s) 305, 566
MbiI CCGCTC 1 cut(s) 45
MboI GATC 2 cut(s) 303, 564
MboII GAAGA 4 cut(s) 222, 572, 717, 858
MfeI CAATTG 1 cut(s) 423
MhlI GDGCHC 1 cut(s) 230
MluCI AATT 5 cut(s) 10, 423, 595, 797, 850
MlyI GAGTC 5 cut(s) 13, 145, 209, 338, 877
MmeI TCCRAC 2 cut(s) 183, 433
MnlI CCTC 8 cut(s) 63, 66, 75, 253, 539, 546, 601, 752
MroI TCCGGA 1 cut(s) 160
MroXI GAANNNNTTC 2 cut(s) 645, 850
MseI TTAA 1 cut(s) 9
MspA1I CMGCKG 2 cut(s) 470, 830
MspI CCGG 1 cut(s) 161
MspR9I CCNGG 2 cut(s) 127, 390
MunI CAATTG 1 cut(s) 423
MvaI CCWGG 2 cut(s) 127, 390
MvnI CGCG 1 cut(s) 29
NdeI CATATG 1 cut(s) 345
NdeII GATC 2 cut(s) 303, 564
NlaIII CATG 4 cut(s) 314, 358, 434, 758
NlaIV GGNNCC 2 cut(s) 33, 324
NmuCI GTSAC 1 cut(s) 380
PciSI GCTCTTC 1 cut(s) 235
PcsI WCGNNNNNNNCGW 3 cut(s) 209, 218, 230
PdmI GAANNNNTTC 2 cut(s) 645, 850
PfeI GAWTC 2 cut(s) 113, 646
PflFI GACNNNGTC 1 cut(s) 167
PflMI CCANNNNNTGG 1 cut(s) 473
PkrI GCNGC 5 cut(s) 26, 31, 183, 514, 829
PleI GAGTC 5 cut(s) 13, 145, 208, 337, 876
PpsI GAGTC 5 cut(s) 13, 145, 208, 337, 876
PpuMI RGGWCCY 1 cut(s) 323
PshAI GACNNNNGTC 1 cut(s) 867
Psp5II RGGWCCY 1 cut(s) 323
Psp6I CCWGG 2 cut(s) 125, 388
PspGI CCWGG 2 cut(s) 125, 388
PspN4I GGNNCC 2 cut(s) 33, 324
PspPI GGNCC 2 cut(s) 84, 323
PspPPI RGGWCCY 1 cut(s) 323
PsyI GACNNNGTC 1 cut(s) 167
PvuII CAGCTG 2 cut(s) 470, 830
RsaI GTAC 1 cut(s) 350
RsaNI GTAC 1 cut(s) 349
SapI GCTCTTC 1 cut(s) 235
SaqAI TTAA 1 cut(s) 9
SatI GCNGC 5 cut(s) 25, 30, 182, 513, 828
Sau3AI GATC 2 cut(s) 303, 564
Sau96I GGNCC 2 cut(s) 84, 323
SchI GAGTC 5 cut(s) 13, 145, 209, 338, 877
ScrFI CCNGG 2 cut(s) 127, 390
SduI GDGCHC 1 cut(s) 230
SinI GGWCC 2 cut(s) 84, 323
SmlI CTYRAG 1 cut(s) 278
SmoI CTYRAG 1 cut(s) 278
Sse9I AATT 5 cut(s) 10, 423, 595, 797, 850
SsiI CCGC 8 cut(s) 29, 43, 99, 123, 181, 246, 352, 789
SspMI CTAG 2 cut(s) 812, 843
StyD4I CCNGG 2 cut(s) 125, 388
TaaI ACNGT 1 cut(s) 267
TaiI ACGT 2 cut(s) 242, 387
TaqI TCGA 1 cut(s) 212
TasI AATT 5 cut(s) 10, 423, 595, 797, 850
TauI GCSGC 2 cut(s) 32, 184
TfiI GAWTC 2 cut(s) 113, 646
Tru1I TTAA 1 cut(s) 9
Tru9I TTAA 1 cut(s) 9
TscAI CASTG 2 cut(s) 42, 270
TseFI GTSAC 1 cut(s) 380
TseI GCWGC 3 cut(s) 24, 512, 827
Tsp45I GTSAC 1 cut(s) 380
TspDTI ATGAA 5 cut(s) 537, 603, 674, 681, 769
TspGWI ACGGA 1 cut(s) 127
TspRI CASTG 2 cut(s) 42, 270
Tth111I GACNNNGTC 1 cut(s) 167
Van91I CCANNNNNTGG 1 cut(s) 473
VpaK11BI GGWCC 2 cut(s) 84, 323
XapI RAATTY 2 cut(s) 797, 850
XbaI TCTAGA 1 cut(s) 842
XmnI GAANNNNTTC 2 cut(s) 645, 850
XspI CTAG 2 cut(s) 812, 843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.