Prupe.1G349600_v2.0.a1

Mur ligase family, glutamate ligase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0018433
Physical Location & Seq
Reverse (-)
32589789 .. 32592430
2642 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G349600.1_v2.0.a1.CDS.3

Sequence Viewer

Length: 1545 bp
ATGTCTGCAACAGCAGCAGCTGGAGCAACACCACACCCCTCCTCCTTCCCTCACTTATTCTCAAATTCAATCATAAAATCAAGGCACTCCATCTCTGTCACCCGTAATTCAATAAACCCTAGCTCCCCAAATACCCCCAATCCCACTTGGACCGCCTCTGAGATCGCCGACGCTGTCAATGGCAGGATTGTGAAATGGGGTCCTCCTGGAACCGTTTCTACAGACACCAGAACCCTTCAGCCCAACCAATGGTTCTTCGCCATTGCCGGTCTAAACTTCGACGCCCACGATTTCATAACCCTGGAATTATCCCGTAATAAAGGCTGTGCCGGAGTTATCGGCAACCGGGTTTGCGAAAATTGGGACAAGGGCTTCGTTCAATTCGATGGCAATACTGTAATTTCATTGACGAAGATGGCAAGTTATGCGAGGAATAGGTTCCATGGAGAAGTTGTGGGAGTGACTGGGAGTGTGGGAAAGACCAGCACAAAATGCATGGTAGCTTTGGCTCTTCAAATTCAAAGTTCAAGAGGTTTGGTTCTTCATGTTCATCAAAGTCGTGGGAATTGGAATAGTGAAATTGGGGTGGCTCTGTCGCTAATTGGGGTTCCTGCAGGAACTGGGATTGTGGTTTTGGAGTTGGGAATGAGTGGGAAGGGGGAGATATTAGAGCTTGCGAGGATGGCTCGGCCCACTATCAGGGTCATTTTAAATGTGGGCGCTTCCCATTTTCAAAATTTCTCCAGTTTAAAGGAGATAGGCATGGCCAAGGGGGAGATTTTGGCCGAAGCAAAGCCCGGGGATGTTTGTGTTTTGAACGCTGATGATCCACTTGTTATGAGCCTTCCTGTACCACTTGGTGTCAAAAAGGTGCTCTTTGGTCAGAGAGTGGGGTGTGATGTTCGGTTGGTCGCGTCAGAAAGCACGGACGGAGGTCTTGGAGTTAGAGTTGTCTTACAGAATCATCAAGAGATGGTAGAATTTGTGATAGCTAGTCCAGGTCTGCATTTGGCTCTCAATGCATGTGCAGCAGCAGCAGTTGCGACCCTTTTAGGAGTCTCTCTTTCTGAAATTGGAAATCGCCTGTCTGCATTTTCTCCTGTTCACATGAGGTCGGAGCTTGAAGTCAGTAGAAATGGAATCAAAATAGTGAATGATGCTTACAATGCCAATCCTGTGAGCACCAAGGCCGCCATTGACTTACTAGAGAGTATTGATATTGATTGCGGAGGCAAAAGAATTGTTGTGCTTGGCGACATGTTAGAACTTGGTGCAATTGAGTTGGAGTCTCACAAGATGATTCTGAAGTGCTGCTGTTGTAGTGCTCATATTGATTTAGTTGGGCTTGTTGGGAGGAGGTTTTGTGCAGCTGCTAAGAATCTCAACTTGCTTGAAGACAGAAATATTGTGTATGCTCTTGACTCAGAAACTTTTGCACAAGAAATTGTAAAGAGGGTTAGTTATAATGATGTTGTTTTGGTAAAGGGTAGTCGAGCAATGCAAATGGAGAAAGTTGTAAATGCAATTAAAGCAATGGAATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mur_ligase_C Mur_ligase_M

Protein Analysis

515

Amino Acids

54.82

Weight (kDa)

7.93

Isoelectric Point (pI)

37.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018433)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g37200
malus_domestica MD13G1002800.v1.1
prunus_persica Prupe.1G349600_v2.0.a1
rosa_laevigata RLG00000005615
rosa_rugosa Rorug04G0376600
rosa_samantha Rh4AG438200 Rh4BG447200 Rh4CG464400 Rh4DG445900
rosa_wichuraiana Rw4G037480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1464
AccB7I CCANNNNNTGG 1 cut(s) 249
AccII CGCG 1 cut(s) 914
AciI CCGC 3 cut(s) 153, 1191, 1227
AclWI GGATC 1 cut(s) 821
AcoI YGGCCR 2 cut(s) 765, 783
AcsI RAATTY 4 cut(s) 64, 516, 736, 980
AcuI CTGAAG 2 cut(s) 221, 1325
AcyI GRCGYC 1 cut(s) 282
AdeI CACNNNGTG 1 cut(s) 860
AfaI GTAC 1 cut(s) 852
AfiI CCNNNNNNNGG 2 cut(s) 249, 699
AflIII ACRYGT 1 cut(s) 1257
AjnI CCWGG 3 cut(s) 205, 300, 997
AjuI GAANNNNNNNTTGG 2 cut(s) 236, 268
AluBI AGCT 7 cut(s) 20, 123, 503, 673, 992, 1120, 1370
AluI AGCT 7 cut(s) 20, 123, 503, 673, 992, 1120, 1370
Alw21I GWGCWC 3 cut(s) 876, 1184, 1327
Alw26I GTCTC 2 cut(s) 1063, 1293
AlwI GGATC 1 cut(s) 821
AlwNI CAGNNNCTG 2 cut(s) 20, 620
Ama87I CYCGRG 1 cut(s) 797
AoxI GGCC 4 cut(s) 689, 765, 783, 1188
ApeKI GCWGC 8 cut(s) 14, 17, 1028, 1031, 1034, 1311, 1367, 1370
ApoI RAATTY 4 cut(s) 64, 516, 736, 980
ArsI GACNNNNNNTTYG 2 cut(s) 356, 388
Asp700I GAANNNNTTC 2 cut(s) 214, 437
AspLEI GCGC 1 cut(s) 722
AspS9I GGNCC 3 cut(s) 150, 200, 690
AsuC2I CCSGG 3 cut(s) 347, 798, 799
AsuHPI GGTGA 1 cut(s) 91
AvaI CYCGRG 1 cut(s) 797
AvaII GGWCC 2 cut(s) 150, 200
BalI TGGCCA 1 cut(s) 767
BbsI GAAGAC 1 cut(s) 1401
Bbv12I GWGCWC 3 cut(s) 876, 1184, 1327
BbvI GCAGC 8 cut(s) 26, 29, 1040, 1043, 1046, 1298, 1357, 1379
BccI CCATC 5 cut(s) 98, 380, 409, 676, 967
BciT130I CCWGG 3 cut(s) 207, 302, 999
BcnI CCSGG 3 cut(s) 347, 798, 799
BcoDI GTCTC 2 cut(s) 1063, 1293
BfaI CTAG 3 cut(s) 120, 993, 1205
BfmI CTRYAG 2 cut(s) 219, 612
BfoI RGCGCY 1 cut(s) 723
BisI GCNGC 9 cut(s) 15, 18, 1029, 1032, 1035, 1191, 1312, 1368, 1371
BlsI GCNGC 9 cut(s) 16, 19, 1030, 1033, 1036, 1192, 1313, 1369, 1372
Bme1390I CCNGG 6 cut(s) 207, 302, 347, 798, 799, 999
Bme18I GGWCC 2 cut(s) 150, 200
BmeT110I CYCGRG 1 cut(s) 797
BmgT120I GGNCC 3 cut(s) 150, 200, 690
BmiI GGNNCC 4 cut(s) 201, 211, 440, 609
BmrFI CCNGG 6 cut(s) 207, 302, 347, 798, 799, 999
BmrI ACTGGG 2 cut(s) 474, 630
BmsI GCATC 1 cut(s) 1147
BmuI ACTGGG 2 cut(s) 474, 630
BoxI GACNNNNGTC 1 cut(s) 933
BpiI GAAGAC 1 cut(s) 1401
BplI GAGNNNNNCTC 2 cut(s) 670, 702
BpmI CTGGAG 2 cut(s) 42, 727
BpuMI CCSGG 3 cut(s) 347, 798, 799
BsaHI GRCGYC 1 cut(s) 282
BsaJI CCNNGG 6 cut(s) 300, 442, 768, 797, 798, 1185
Bsc4I CCNNNNNNNGG 2 cut(s) 249, 699
Bse118I RCCGGY 1 cut(s) 266
Bse1I ACTGG 3 cut(s) 469, 625, 744
Bse3DI GCAATG 3 cut(s) 261, 1503, 1539
BseBI CCWGG 3 cut(s) 207, 302, 999
BseDI CCNNGG 6 cut(s) 300, 442, 768, 797, 798, 1185
BseGI GGATG 2 cut(s) 687, 808
BseLI CCNNNNNNNGG 2 cut(s) 249, 699
BseMI GCAATG 3 cut(s) 261, 1503, 1539
BseMII CTCAG 2 cut(s) 150, 1437
BseNI ACTGG 3 cut(s) 469, 625, 744
BseRI GAGGAG 2 cut(s) 31, 1369
BseXI GCAGC 8 cut(s) 26, 29, 1040, 1043, 1046, 1298, 1357, 1379
BsgI GTGCAG 2 cut(s) 1047, 1386
Bsh1236I CGCG 1 cut(s) 914
BshFI GGCC 4 cut(s) 691, 767, 785, 1190
BsiHKAI GWGCWC 3 cut(s) 876, 1184, 1327
BsiHKCI CYCGRG 1 cut(s) 797
BsiSI CCGG 4 cut(s) 267, 330, 346, 798
BslFI GGGAC 1 cut(s) 377
BslI CCNNNNNNNGG 2 cut(s) 249, 699
BsmAI GTCTC 2 cut(s) 1063, 1293
BsmFI GGGAC 1 cut(s) 377
BsnI GGCC 4 cut(s) 691, 767, 785, 1190
BsoBI CYCGRG 1 cut(s) 797
Bsp1286I GDGCHC 3 cut(s) 876, 1184, 1327
Bsp143I GATC 2 cut(s) 162, 826
Bsp19I CCATGG 1 cut(s) 442
BspACI CCGC 3 cut(s) 153, 1191, 1227
BspANI GGCC 4 cut(s) 691, 767, 785, 1190
BspCNI CTCAG 2 cut(s) 151, 1436
BspFNI CGCG 1 cut(s) 914
BspLI GGNNCC 4 cut(s) 201, 211, 440, 609
BspMAI CTGCAG 1 cut(s) 616
BspPI GGATC 1 cut(s) 821
BspQI GCTCTTC 1 cut(s) 516
BsrDI GCAATG 3 cut(s) 261, 1503, 1539
BsrFI RCCGGY 1 cut(s) 266
BsrI ACTGG 3 cut(s) 469, 625, 744
BssAI RCCGGY 1 cut(s) 266
BssECI CCNNGG 6 cut(s) 300, 442, 768, 797, 798, 1185
BssMI GATC 2 cut(s) 162, 826
BssNI GRCGYC 1 cut(s) 282
BssT1I CCWWGG 3 cut(s) 442, 768, 1185
Bst2UI CCWGG 3 cut(s) 207, 302, 999
Bst4CI ACNGT 2 cut(s) 214, 397
Bst6I CTCTTC 1 cut(s) 516
BstACI GRCGYC 1 cut(s) 282
BstAPI GCANNNNNTGC 3 cut(s) 425, 492, 1040
BstC8I GCNNGC 1 cut(s) 675
BstDEI CTNAG 3 cut(s) 159, 1374, 1423
BstDSI CCRYGG 1 cut(s) 442
BstF5I GGATG 2 cut(s) 687, 808
BstFNI CGCG 1 cut(s) 914
BstH2I RGCGCY 1 cut(s) 723
BstHHI GCGC 1 cut(s) 722
BstKTI GATC 2 cut(s) 165, 829
BstMAI GTCTC 2 cut(s) 1063, 1293
BstMBI GATC 2 cut(s) 162, 826
BstNI CCWGG 3 cut(s) 207, 302, 999
BstNSI RCATGY 2 cut(s) 1026, 1261
BstPAI GACNNNNGTC 1 cut(s) 933
BstSCI CCNGG 6 cut(s) 205, 300, 345, 796, 797, 997
BstSFI CTRYAG 2 cut(s) 219, 612
BstUI CGCG 1 cut(s) 914
BstV1I GCAGC 8 cut(s) 26, 29, 1040, 1043, 1046, 1298, 1357, 1379
BstV2I GAAGAC 1 cut(s) 1401
BsuRI GGCC 4 cut(s) 691, 767, 785, 1190
BtgI CCRYGG 1 cut(s) 442
BtsCI GGATG 2 cut(s) 687, 808
Cac8I GCNNGC 1 cut(s) 675
CaiI CAGNNNCTG 2 cut(s) 20, 620
CfoI GCGC 1 cut(s) 722
Cfr10I RCCGGY 1 cut(s) 266
Cfr13I GGNCC 3 cut(s) 150, 200, 690
Cfr9I CCCGGG 1 cut(s) 797
CseI GACGC 3 cut(s) 179, 290, 903
Csp6I GTAC 1 cut(s) 851
CviAII CATG 7 cut(s) 443, 496, 545, 763, 1023, 1108, 1258
CviQI GTAC 1 cut(s) 851
DdeI CTNAG 3 cut(s) 159, 1374, 1423
DpnI GATC 2 cut(s) 164, 828
DpnII GATC 2 cut(s) 162, 826
DraI TTTAAA 2 cut(s) 711, 750
DraIII CACNNNGTG 1 cut(s) 860
EaeI YGGCCR 2 cut(s) 765, 783
Eam1104I CTCTTC 1 cut(s) 516
EarI CTCTTC 1 cut(s) 516
Eco130I CCWWGG 3 cut(s) 442, 768, 1185
Eco47I GGWCC 2 cut(s) 150, 200
Eco57I CTGAAG 2 cut(s) 221, 1325
Eco88I CYCGRG 1 cut(s) 797
EcoO109I RGGNCCY 1 cut(s) 200
EcoRII CCWGG 3 cut(s) 205, 300, 997
EcoT14I CCWWGG 3 cut(s) 442, 768, 1185
EcoT22I ATGCAT 2 cut(s) 497, 1024
ErhI CCWWGG 3 cut(s) 442, 768, 1185
FaeI CATG 7 cut(s) 446, 499, 548, 766, 1026, 1111, 1261
FalI AAGNNNNNCTT 2 cut(s) 860, 892
FaqI GGGAC 1 cut(s) 377
FatI CATG 7 cut(s) 442, 495, 544, 762, 1022, 1107, 1257
Fnu4HI GCNGC 9 cut(s) 15, 18, 1029, 1032, 1035, 1191, 1312, 1368, 1371
FokI GGATG 2 cut(s) 694, 815
Fsp4HI GCNGC 9 cut(s) 15, 18, 1029, 1032, 1035, 1191, 1312, 1368, 1371
FspBI CTAG 3 cut(s) 120, 993, 1205
GlaI GCGC 1 cut(s) 721
GluI GCNGC 9 cut(s) 15, 18, 1029, 1032, 1035, 1191, 1312, 1368, 1371
GsuI CTGGAG 2 cut(s) 42, 727
HaeII RGCGCY 1 cut(s) 723
HaeIII GGCC 4 cut(s) 691, 767, 785, 1190
HapII CCGG 4 cut(s) 267, 330, 346, 798
HgaI GACGC 3 cut(s) 179, 290, 903
HhaI GCGC 1 cut(s) 722
Hin1I GRCGYC 1 cut(s) 282
Hin1II CATG 7 cut(s) 446, 499, 548, 766, 1026, 1111, 1261
Hin6I GCGC 1 cut(s) 720
HinP1I GCGC 1 cut(s) 720
HinfI GANTC 7 cut(s) 961, 1056, 1140, 1286, 1300, 1378, 1421
HpaII CCGG 4 cut(s) 267, 330, 346, 798
HphI GGTGA 1 cut(s) 91
Hpy166II GTNNAC 1 cut(s) 1105
Hpy188I TCNGA 7 cut(s) 160, 885, 919, 1069, 1117, 1305, 1426
Hpy188III TCNNGA 3 cut(s) 528, 968, 1418
Hpy8I GTNNAC 1 cut(s) 1105
Hpy99I CGWCG 2 cut(s) 173, 284
HpyAV CCTTC 4 cut(s) 55, 245, 649, 854
HpyCH4III ACNGT 2 cut(s) 214, 397
HpyF3I CTNAG 3 cut(s) 159, 1374, 1423
Hsp92I GRCGYC 1 cut(s) 282
Hsp92II CATG 7 cut(s) 446, 499, 548, 766, 1026, 1111, 1261
HspAI GCGC 1 cut(s) 720
Kzo9I GATC 2 cut(s) 162, 826
LguI GCTCTTC 1 cut(s) 516
LmnI GCTCC 3 cut(s) 23, 128, 1117
Lsp1109I GCAGC 8 cut(s) 26, 29, 1040, 1043, 1046, 1298, 1357, 1379
LweI GCATC 1 cut(s) 1147
MaeI CTAG 3 cut(s) 120, 993, 1205
MaeIII GTNAC 2 cut(s) 97, 460
MalI GATC 2 cut(s) 164, 828
MboI GATC 2 cut(s) 162, 826
MboII GAAGA 5 cut(s) 247, 424, 503, 533, 1406
MfeI CAATTG 1 cut(s) 1275
MhlI GDGCHC 3 cut(s) 876, 1184, 1327
MlsI TGGCCA 1 cut(s) 767
MluNI TGGCCA 1 cut(s) 767
MlyI GAGTC 3 cut(s) 1065, 1295, 1415
MmeI TCCRAC 2 cut(s) 1095, 1263
Mox20I TGGCCA 1 cut(s) 767
Mph1103I ATGCAT 2 cut(s) 497, 1024
MroXI GAANNNNTTC 2 cut(s) 214, 437
MscI TGGCCA 1 cut(s) 767
MseI TTAA 3 cut(s) 710, 749, 1527
Msp20I TGGCCA 1 cut(s) 767
MspA1I CMGCKG 2 cut(s) 20, 1370
MspI CCGG 4 cut(s) 267, 330, 346, 798
MspR9I CCNGG 6 cut(s) 207, 302, 347, 798, 799, 999
MunI CAATTG 1 cut(s) 1275
MvaI CCWGG 3 cut(s) 207, 302, 999
MvnI CGCG 1 cut(s) 914
NciI CCSGG 3 cut(s) 347, 798, 799
NcoI CCATGG 1 cut(s) 442
NdeII GATC 2 cut(s) 162, 826
NlaIII CATG 7 cut(s) 446, 499, 548, 766, 1026, 1111, 1261
NlaIV GGNNCC 4 cut(s) 201, 211, 440, 609
NmeAIII GCCGAG 1 cut(s) 667
NmuCI GTSAC 2 cut(s) 97, 460
NsiI ATGCAT 2 cut(s) 497, 1024
NspI RCATGY 2 cut(s) 1026, 1261
PciI ACATGT 1 cut(s) 1257
PciSI GCTCTTC 1 cut(s) 516
PcsI WCGNNNNNNNCGW 2 cut(s) 285, 381
PdmI GAANNNNTTC 2 cut(s) 214, 437
PfeI GAWTC 4 cut(s) 961, 1140, 1300, 1378
PflFI GACNNNGTC 1 cut(s) 173
PflMI CCANNNNNTGG 1 cut(s) 249
PfoI TCCNGGA 1 cut(s) 205
PkrI GCNGC 9 cut(s) 16, 19, 1030, 1033, 1036, 1192, 1313, 1369, 1372
PleI GAGTC 3 cut(s) 1064, 1294, 1415
PpsI GAGTC 3 cut(s) 1064, 1294, 1415
PpuMI RGGWCCY 1 cut(s) 200
PscI ACATGT 1 cut(s) 1257
PshAI GACNNNNGTC 1 cut(s) 933
PsiI TTATAA 1 cut(s) 1464
Psp5II RGGWCCY 1 cut(s) 200
Psp6I CCWGG 3 cut(s) 205, 300, 997
PspGI CCWGG 3 cut(s) 205, 300, 997
PspN4I GGNNCC 4 cut(s) 201, 211, 440, 609
PspPI GGNCC 3 cut(s) 150, 200, 690
PspPPI RGGWCCY 1 cut(s) 200
PsrI GAACNNNNNNTAC 2 cut(s) 202, 234
PstI CTGCAG 1 cut(s) 616
PstNI CAGNNNCTG 2 cut(s) 20, 620
PsyI GACNNNGTC 1 cut(s) 173
PvuII CAGCTG 2 cut(s) 20, 1370
RsaI GTAC 1 cut(s) 852
RsaNI GTAC 1 cut(s) 851
SapI GCTCTTC 1 cut(s) 516
SaqAI TTAA 3 cut(s) 710, 749, 1527
SatI GCNGC 9 cut(s) 15, 18, 1029, 1032, 1035, 1191, 1312, 1368, 1371
Sau3AI GATC 2 cut(s) 162, 826
Sau96I GGNCC 3 cut(s) 150, 200, 690
SbfI CCTGCAGG 1 cut(s) 616
SchI GAGTC 3 cut(s) 1065, 1295, 1415
ScrFI CCNGG 6 cut(s) 207, 302, 347, 798, 799, 999
SdaI CCTGCAGG 1 cut(s) 616
SduI GDGCHC 3 cut(s) 876, 1184, 1327
SfaNI GCATC 1 cut(s) 1147
SfcI CTRYAG 2 cut(s) 219, 612
SinI GGWCC 2 cut(s) 150, 200
SmaI CCCGGG 1 cut(s) 799
Sse8387I CCTGCAGG 1 cut(s) 616
SsiI CCGC 3 cut(s) 153, 1191, 1227
SspI AATATT 1 cut(s) 1405
SspMI CTAG 3 cut(s) 120, 993, 1205
StyD4I CCNGG 6 cut(s) 205, 300, 345, 796, 797, 997
StyI CCWWGG 3 cut(s) 442, 768, 1185
TaaI ACNGT 2 cut(s) 214, 397
TaqI TCGA 3 cut(s) 279, 384, 1492
TauI GCSGC 1 cut(s) 1193
TfiI GAWTC 4 cut(s) 961, 1140, 1300, 1378
Tru1I TTAA 3 cut(s) 710, 749, 1527
Tru9I TTAA 3 cut(s) 710, 749, 1527
TseFI GTSAC 2 cut(s) 97, 460
TseI GCWGC 8 cut(s) 14, 17, 1028, 1031, 1034, 1311, 1367, 1370
Tsp45I GTSAC 2 cut(s) 97, 460
TspDTI ATGAA 4 cut(s) 283, 393, 533, 539
TspGWI ACGGA 2 cut(s) 941, 945
TspMI CCCGGG 1 cut(s) 797
Tth111I GACNNNGTC 1 cut(s) 173
Van91I CCANNNNNTGG 1 cut(s) 249
VpaK11BI GGWCC 2 cut(s) 150, 200
XapI RAATTY 4 cut(s) 64, 516, 736, 980
XceI RCATGY 2 cut(s) 1026, 1261
XmaI CCCGGG 1 cut(s) 797
XmnI GAANNNNTTC 2 cut(s) 214, 437
XspI CTAG 3 cut(s) 120, 993, 1205
Zsp2I ATGCAT 2 cut(s) 497, 1024
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.