Prupe.1G458800_v2.0.a1

Sarcosine oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0013564
Physical Location & Seq
Reverse (-)
38416723 .. 38417922
1200 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G458800.1

Sequence Viewer

Length: 1137 bp
ATGGAATATTCCGCCGATGAATTCGACGTCATCGTGGTCGGAGCCGGCATCATGGGTAGCTCCACCGCCTACCAGACGGCCAAGCGTGACCAGAAGACCCTCTTCCTCGAGCAATTCGACTTCCTCCACCACCGCGGCTCCTCCCGTGGCGCGGTTGGCGAGTCCTGGACGATCCGCGCCACTTACCCAGAAGCCGAGTCCGAGATCGGCTACAATGTCTACTTCAAAGTTCACCATTTGGACATGGCCCCGGCAAACAACAAAGTTCTGCATGCCATTGTTGAGAGCTGTGGTAAAAATTCGGTCCCCTTTCGGGTCATGAAGCGGGACCAGCTGGATCGGGAGTTCTGGGGTCGGGTTCGGATTTCGAAGGATTGGGTGGCCGTGGCGACGGAGCATGGTGAGGTTATTAAACCCACCAAGGCTAATGGTGCCGTTTTGAGGGACAATATGGAGGTGAAGGGTGTGGAGAGAGATGGGGTGAGAAAGGGGGTTTGGGTGTGCACAGAAAATGGAGAGAGGTTTTGGGGGAAGAAGTGTGTGGTGACCGTTGGGGCTTGGACTACAAAGTTAGTTAAAACGGTTGGTGGAATTGAGCTGCCTATGCAGCCATTGGAGACTGCTGTTTGTTATTGGAGGATTAAGGAGGGGCATGAGGGTGGTTTTGCCATTGGTGGTGACTTTCCAACCTTTGCTAGCTATGGGGATACCTACATTTATGGGACACTCTCTTTGGAGTACCCCGGTTTGATCAAGGTTGCCGTGCATGGCGGGTACCCATGTGACCCGGATAAGAGGCCGTGGGGACCCGGGAACCCGCTGGCTCCGTTGAAGGAGTGGATTGAGGGGAGGTTCTCCGGTGTGGTTGACTCCGGTGGGCCGGTGGCCACTCAGCTGTGTATGTACTCCATGACCCCGGATGAGGACTTTGTGATTGATTTCTTGGGTGGGGAGTTTGGGAAGGATGTGGTGGTGGGTGGCGGGTTTTCGGGTCACGGGTTCAAGCTGTCTCCGGTGGTGGGGAGGATATTGGCTGACCTCGCACTTAGTGGGGAGGCTCAAGGAGTGGAGCTAAAGCACTTTAGGATAGCAAGGTTTCAAGAGAATCCTAAAGGCAATGTTAAAGACTTCCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008115 GO:0008150 GO:0008152 GO:0016491 GO:0016645 GO:0016647 GO:0055114
KEGG Pathways
Metabolic & Signaling
ko00260 ko00310 ko01100 ko04146 map00260 map00310 map01100 map04146
Pfam Domains
Protein Families
DAO

Protein Analysis

379

Amino Acids

41.62

Weight (kDa)

6.1

Isoelectric Point (pI)

18.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013564)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24580
fragaria_vesca FvH4_2g36660
malus_domestica MD08G1126500.v1.1 MD15G1106400.v1.1
prunus_persica Prupe.1G458600_v2.0.a1 Prupe.1G458800_v2.0.a1 Prupe.1G458900_v2.0.a1
pyrus_communis pycom08g10570 pycom15g09710
rosa_chinensis RchiOBHm_Chr6g0305131
rosa_laevigata RLG00000010916
rosa_multiflora Rmu_sc0000061.1_g000020
rosa_rugosa Rorug06G0337900
rosa_samantha Rh6AG449500 Rh6BG451800 Rh6CG463000 Rh6DG450000
rosa_wichuraiana Rw6G039150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 30
Acc65I GGTACC 1 cut(s) 774
AccB1I GGYRCC 2 cut(s) 431, 774
AccI GTMKAC 1 cut(s) 219
AccII CGCG 3 cut(s) 135, 152, 177
AclWI GGATC 2 cut(s) 166, 345
AcoI YGGCCR 3 cut(s) 78, 381, 885
AcsI RAATTY 2 cut(s) 20, 298
AcyI GRCGYC 1 cut(s) 27
AdeI CACNNNGTG 1 cut(s) 1049
AfaI GTAC 3 cut(s) 740, 776, 905
AfiI CCNNNNNNNGG 5 cut(s) 151, 313, 441, 922, 1019
AgsI TTSAA 4 cut(s) 226, 832, 1003, 1100
AjnI CCWGG 1 cut(s) 164
AjuI GAANNNNNNNTTGG 2 cut(s) 478, 510
AloI GAACNNNNNNTCC 2 cut(s) 329, 361
AluBI AGCT 8 cut(s) 60, 288, 334, 598, 699, 895, 1006, 1072
AluI AGCT 8 cut(s) 60, 288, 334, 598, 699, 895, 1006, 1072
Alw21I GWGCWC 1 cut(s) 506
Alw26I GTCTC 2 cut(s) 611, 1014
Alw44I GTGCAC 1 cut(s) 502
AlwI GGATC 2 cut(s) 166, 345
Ama87I CYCGRG 2 cut(s) 107, 809
AoxI GGCC 6 cut(s) 78, 246, 381, 797, 878, 885
ApaLI GTGCAC 1 cut(s) 502
ApeKI GCWGC 2 cut(s) 598, 607
ApoI RAATTY 2 cut(s) 20, 298
ArsI GACNNNNNNTTYG 2 cut(s) 715, 747
Asp718I GGTACC 1 cut(s) 774
AspLEI GCGC 2 cut(s) 152, 179
AspS9I GGNCC 5 cut(s) 247, 304, 328, 806, 878
AsuC2I CCSGG 6 cut(s) 251, 744, 788, 810, 811, 917
AsuHPI GGTGA 6 cut(s) 224, 413, 469, 493, 556, 689
AsuII TTCGAA 1 cut(s) 368
AsuNHI GCTAGC 1 cut(s) 695
AvaI CYCGRG 2 cut(s) 107, 809
AvaII GGWCC 3 cut(s) 304, 328, 806
BaeGI GKGCMC 1 cut(s) 506
BaeI ACNNNNGTAYC 2 cut(s) 766, 799
BalI TGGCCA 1 cut(s) 887
BanI GGYRCC 2 cut(s) 431, 774
BbsI GAAGAC 1 cut(s) 101
Bbv12I GWGCWC 1 cut(s) 506
BbvI GCAGC 2 cut(s) 585, 619
BccI CCATC 1 cut(s) 470
BceAI ACGGC 5 cut(s) 93, 368, 419, 746, 784
BciT130I CCWGG 1 cut(s) 166
BciVI GTATCC 1 cut(s) 700
BclI TGATCA 1 cut(s) 750
BcnI CCSGG 6 cut(s) 251, 744, 788, 810, 811, 917
BcoDI GTCTC 2 cut(s) 611, 1014
BfaI CTAG 1 cut(s) 696
BfmI CTRYAG 1 cut(s) 1133
BfuI GTATCC 1 cut(s) 700
BisI GCNGC 3 cut(s) 136, 599, 608
BlsI GCNGC 3 cut(s) 137, 600, 609
Bme1390I CCNGG 7 cut(s) 166, 251, 744, 788, 810, 811, 917
Bme18I GGWCC 3 cut(s) 304, 328, 806
BmeT110I CYCGRG 2 cut(s) 107, 809
BmgT120I GGNCC 5 cut(s) 247, 304, 328, 806, 878
BmrFI CCNGG 7 cut(s) 166, 251, 744, 788, 810, 811, 917
BmsI GCATC 1 cut(s) 57
BmtI GCTAGC 1 cut(s) 699
BpiI GAAGAC 1 cut(s) 101
Bpu14I TTCGAA 1 cut(s) 368
BpuEI CTTGAG 1 cut(s) 1044
BpuMI CCSGG 6 cut(s) 251, 744, 788, 810, 811, 917
BsaHI GRCGYC 1 cut(s) 27
BsaJI CCNNGG 9 cut(s) 133, 145, 249, 384, 420, 742, 800, 809, 915
BsaWI WCCGGW 3 cut(s) 857, 872, 1012
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 5 cut(s) 151, 313, 441, 922, 1019
Bse118I RCCGGY 2 cut(s) 44, 880
Bse3DI GCAATG 1 cut(s) 1123
BseBI CCWGG 1 cut(s) 166
BseDI CCNNGG 9 cut(s) 133, 145, 249, 384, 420, 742, 800, 809, 915
BseGI GGATG 2 cut(s) 925, 970
BseLI CCNNNNNNNGG 5 cut(s) 151, 313, 441, 922, 1019
BseMI GCAATG 1 cut(s) 1123
BseMII CTCAG 1 cut(s) 905
BseRI GAGGAG 1 cut(s) 130
BseSI GKGCMC 1 cut(s) 506
BseXI GCAGC 2 cut(s) 585, 619
Bsh1236I CGCG 3 cut(s) 135, 152, 177
BshFI GGCC 6 cut(s) 80, 248, 383, 799, 880, 887
BshNI GGYRCC 2 cut(s) 431, 774
BsiHKAI GWGCWC 1 cut(s) 506
BsiHKCI CYCGRG 2 cut(s) 107, 809
BslFI GGGAC 5 cut(s) 290, 341, 458, 736, 819
BslI CCNNNNNNNGG 5 cut(s) 151, 313, 441, 922, 1019
BsmAI GTCTC 2 cut(s) 611, 1014
BsmFI GGGAC 5 cut(s) 290, 341, 458, 736, 819
BsnI GGCC 6 cut(s) 80, 248, 383, 799, 880, 887
BsoBI CYCGRG 2 cut(s) 107, 809
Bsp119I TTCGAA 1 cut(s) 368
Bsp1286I GDGCHC 1 cut(s) 506
Bsp143I GATC 4 cut(s) 171, 204, 337, 750
BspANI GGCC 6 cut(s) 80, 248, 383, 799, 880, 887
BspCNI CTCAG 1 cut(s) 904
BspFNI CGCG 3 cut(s) 135, 152, 177
BspHI TCATGA 1 cut(s) 318
BspOI GCTAGC 1 cut(s) 699
BspPI GGATC 2 cut(s) 166, 345
BspT104I TTCGAA 1 cut(s) 368
BspT107I GGYRCC 2 cut(s) 431, 774
BsrDI GCAATG 1 cut(s) 1123
BsrFI RCCGGY 2 cut(s) 44, 880
BssAI RCCGGY 2 cut(s) 44, 880
BssECI CCNNGG 9 cut(s) 133, 145, 249, 384, 420, 742, 800, 809, 915
BssMI GATC 4 cut(s) 171, 204, 337, 750
BssNI GRCGYC 1 cut(s) 27
BssT1I CCWWGG 1 cut(s) 420
Bst2UI CCWGG 1 cut(s) 166
Bst4CI ACNGT 2 cut(s) 550, 583
Bst6I CTCTTC 1 cut(s) 107
BstACI GRCGYC 1 cut(s) 27
BstBI TTCGAA 1 cut(s) 368
BstC8I GCNNGC 4 cut(s) 46, 273, 697, 822
BstDEI CTNAG 2 cut(s) 891, 1046
BstDSI CCRYGG 4 cut(s) 133, 145, 384, 800
BstEII GGTNACC 1 cut(s) 544
BstF5I GGATG 2 cut(s) 925, 970
BstFNI CGCG 3 cut(s) 135, 152, 177
BstHHI GCGC 2 cut(s) 152, 179
BstKTI GATC 4 cut(s) 174, 207, 340, 753
BstMAI GTCTC 2 cut(s) 611, 1014
BstMBI GATC 4 cut(s) 171, 204, 337, 750
BstMWI GCNNNNNNNGC 6 cut(s) 156, 331, 431, 604, 607, 1040
BstNI CCWGG 1 cut(s) 166
BstNSI RCATGY 1 cut(s) 275
BstPI GGTNACC 1 cut(s) 544
BstSCI CCNGG 7 cut(s) 164, 249, 742, 786, 808, 809, 915
BstSFI CTRYAG 1 cut(s) 1133
BstSLI GKGCMC 1 cut(s) 506
BstUI CGCG 3 cut(s) 135, 152, 177
BstV1I GCAGC 2 cut(s) 585, 619
BstV2I GAAGAC 1 cut(s) 101
BsuI GTATCC 1 cut(s) 700
BsuRI GGCC 6 cut(s) 80, 248, 383, 799, 880, 887
BtgI CCRYGG 4 cut(s) 133, 145, 384, 800
BtsCI GGATG 2 cut(s) 925, 970
Cac8I GCNNGC 4 cut(s) 46, 273, 697, 822
CciI TCATGA 1 cut(s) 318
CfoI GCGC 2 cut(s) 152, 179
Cfr10I RCCGGY 2 cut(s) 44, 880
Cfr13I GGNCC 5 cut(s) 247, 304, 328, 806, 878
Cfr42I CCGCGG 1 cut(s) 136
Cfr9I CCCGGG 1 cut(s) 809
Csp6I GTAC 3 cut(s) 739, 775, 904
CviAII CATG 9 cut(s) 52, 244, 272, 319, 398, 653, 767, 780, 910
CviQI GTAC 3 cut(s) 739, 775, 904
DdeI CTNAG 2 cut(s) 891, 1046
DpnI GATC 4 cut(s) 173, 206, 339, 752
DpnII GATC 4 cut(s) 171, 204, 337, 750
DraIII CACNNNGTG 1 cut(s) 1049
EaeI YGGCCR 3 cut(s) 78, 381, 885
Eam1104I CTCTTC 1 cut(s) 107
EarI CTCTTC 1 cut(s) 107
Eco130I CCWWGG 1 cut(s) 420
Eco47I GGWCC 3 cut(s) 304, 328, 806
Eco88I CYCGRG 2 cut(s) 107, 809
Eco91I GGTNACC 1 cut(s) 544
EcoO109I RGGNCCY 1 cut(s) 806
EcoO65I GGTNACC 1 cut(s) 544
EcoRI GAATTC 1 cut(s) 20
EcoRII CCWGG 1 cut(s) 164
EcoT14I CCWWGG 1 cut(s) 420
ErhI CCWWGG 1 cut(s) 420
FaeI CATG 9 cut(s) 55, 247, 275, 322, 401, 656, 770, 783, 913
FalI AAGNNNNNCTT 2 cut(s) 86, 118
FaqI GGGAC 5 cut(s) 290, 341, 458, 736, 819
FatI CATG 9 cut(s) 51, 243, 271, 318, 397, 652, 766, 779, 909
FauI CCCGC 4 cut(s) 318, 764, 825, 974
FbaI TGATCA 1 cut(s) 750
FblI GTMKAC 1 cut(s) 219
Fnu4HI GCNGC 3 cut(s) 136, 599, 608
FokI GGATG 2 cut(s) 932, 977
Fsp4HI GCNGC 3 cut(s) 136, 599, 608
FspBI CTAG 1 cut(s) 696
GlaI GCGC 2 cut(s) 151, 178
GluI GCNGC 3 cut(s) 136, 599, 608
HaeIII GGCC 6 cut(s) 80, 248, 383, 799, 880, 887
HhaI GCGC 2 cut(s) 152, 179
Hin1I GRCGYC 1 cut(s) 27
Hin1II CATG 9 cut(s) 55, 247, 275, 322, 401, 656, 770, 783, 913
Hin6I GCGC 2 cut(s) 150, 177
HinP1I GCGC 2 cut(s) 150, 177
HincII GTYRAC 1 cut(s) 868
HindII GTYRAC 1 cut(s) 868
HinfI GANTC 4 cut(s) 161, 197, 869, 1105
HphI GGTGA 6 cut(s) 224, 413, 469, 493, 556, 689
Hpy166II GTNNAC 4 cut(s) 220, 232, 504, 868
Hpy188I TCNGA 3 cut(s) 41, 202, 363
Hpy188III TCNNGA 3 cut(s) 319, 341, 1100
Hpy8I GTNNAC 4 cut(s) 220, 232, 504, 868
Hpy99I CGWCG 2 cut(s) 29, 394
HpyAV CCTTC 4 cut(s) 364, 454, 826, 955
HpyCH4III ACNGT 2 cut(s) 550, 583
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 4 cut(s) 271, 504, 607, 766
HpyF10VI GCNNNNNNNGC 6 cut(s) 156, 331, 431, 604, 607, 1040
HpyF3I CTNAG 2 cut(s) 891, 1046
HpySE526I ACGT 1 cut(s) 27
Hsp92I GRCGYC 1 cut(s) 27
Hsp92II CATG 9 cut(s) 55, 247, 275, 322, 401, 656, 770, 783, 913
HspAI GCGC 2 cut(s) 150, 177
KflI GGGWCCC 1 cut(s) 806
KpnI GGTACC 1 cut(s) 778
KroI GCCGGC 1 cut(s) 44
KroNI GCCGGC 1 cut(s) 46
Ksp22I TGATCA 1 cut(s) 750
KspI CCGCGG 1 cut(s) 136
Kzo9I GATC 4 cut(s) 171, 204, 337, 750
LmnI GCTCC 6 cut(s) 41, 65, 143, 394, 829, 1069
Lsp1109I GCAGC 2 cut(s) 585, 619
LweI GCATC 1 cut(s) 57
MaeI CTAG 1 cut(s) 696
MaeII ACGT 1 cut(s) 27
MaeIII GTNAC 5 cut(s) 86, 544, 677, 782, 992
MalI GATC 4 cut(s) 173, 206, 339, 752
MboI GATC 4 cut(s) 171, 204, 337, 750
MboII GAAGA 3 cut(s) 94, 106, 544
MhlI GDGCHC 1 cut(s) 506
MlsI TGGCCA 1 cut(s) 887
MluCI AATT 4 cut(s) 20, 113, 298, 591
MluNI TGGCCA 1 cut(s) 887
MlyI GAGTC 3 cut(s) 170, 206, 863
MmeI TCCRAC 2 cut(s) 19, 710
Mox20I TGGCCA 1 cut(s) 887
MroNI GCCGGC 1 cut(s) 44
MscI TGGCCA 1 cut(s) 887
MseI TTAA 4 cut(s) 411, 576, 642, 1122
MslI CAYNNNNRTG 1 cut(s) 657
Msp20I TGGCCA 1 cut(s) 887
MspA1I CMGCKG 4 cut(s) 135, 334, 820, 895
MspR9I CCNGG 7 cut(s) 166, 251, 744, 788, 810, 811, 917
MvaI CCWGG 1 cut(s) 166
MvnI CGCG 3 cut(s) 135, 152, 177
MwoI GCNNNNNNNGC 6 cut(s) 156, 331, 431, 604, 607, 1040
NaeI GCCGGC 1 cut(s) 46
NciI CCSGG 6 cut(s) 251, 744, 788, 810, 811, 917
NdeII GATC 4 cut(s) 171, 204, 337, 750
NgoMIV GCCGGC 1 cut(s) 44
NheI GCTAGC 1 cut(s) 695
NlaIII CATG 9 cut(s) 55, 247, 275, 322, 401, 656, 770, 783, 913
NmeAIII GCCGAG 1 cut(s) 220
NmuCI GTSAC 5 cut(s) 86, 544, 677, 782, 992
NspI RCATGY 1 cut(s) 275
NspV TTCGAA 1 cut(s) 368
PaeI GCATGC 1 cut(s) 275
PaeR7I CTCGAG 1 cut(s) 107
PagI TCATGA 1 cut(s) 318
PcsI WCGNNNNNNNCGW 2 cut(s) 30, 114
PdiI GCCGGC 1 cut(s) 46
PfeI GAWTC 1 cut(s) 1105
PfoI TCCNGGA 1 cut(s) 164
PkrI GCNGC 3 cut(s) 137, 600, 609
PleI GAGTC 3 cut(s) 169, 205, 863
PpsI GAGTC 3 cut(s) 169, 205, 863
PpuMI RGGWCCY 1 cut(s) 806
Psp5II RGGWCCY 1 cut(s) 806
Psp6I CCWGG 1 cut(s) 164
PspEI GGTNACC 1 cut(s) 544
PspGI CCWGG 1 cut(s) 164
PspPI GGNCC 5 cut(s) 247, 304, 328, 806, 878
PspPPI RGGWCCY 1 cut(s) 806
PspXI VCTCGAGB 1 cut(s) 107
PvuII CAGCTG 2 cut(s) 334, 895
RsaI GTAC 3 cut(s) 740, 776, 905
RsaNI GTAC 3 cut(s) 739, 775, 904
RseI CAYNNNNRTG 1 cut(s) 657
SacII CCGCGG 1 cut(s) 136
SaqAI TTAA 4 cut(s) 411, 576, 642, 1122
SatI GCNGC 3 cut(s) 136, 599, 608
Sau3AI GATC 4 cut(s) 171, 204, 337, 750
Sau96I GGNCC 5 cut(s) 247, 304, 328, 806, 878
SchI GAGTC 3 cut(s) 170, 206, 863
ScrFI CCNGG 7 cut(s) 166, 251, 744, 788, 810, 811, 917
SduI GDGCHC 1 cut(s) 506
SfaNI GCATC 1 cut(s) 57
SfcI CTRYAG 1 cut(s) 1133
Sfr274I CTCGAG 1 cut(s) 107
Sfr303I CCGCGG 1 cut(s) 136
SfuI TTCGAA 1 cut(s) 368
SgrBI CCGCGG 1 cut(s) 136
SinI GGWCC 3 cut(s) 304, 328, 806
SlaI CTCGAG 1 cut(s) 107
SmaI CCCGGG 1 cut(s) 811
SmiMI CAYNNNNRTG 1 cut(s) 657
SmlI CTYRAG 2 cut(s) 107, 1059
SmoI CTYRAG 2 cut(s) 107, 1059
SphI GCATGC 1 cut(s) 275
Sse9I AATT 4 cut(s) 20, 113, 298, 591
SspI AATATT 1 cut(s) 8
SspMI CTAG 1 cut(s) 696
StyD4I CCNGG 7 cut(s) 164, 249, 742, 786, 808, 809, 915
StyI CCWWGG 1 cut(s) 420
TaaI ACNGT 2 cut(s) 550, 583
TaiI ACGT 1 cut(s) 30
TaqI TCGA 4 cut(s) 24, 108, 117, 368
TaqII GACCGA 1 cut(s) 292
TasI AATT 4 cut(s) 20, 113, 298, 591
TatI WGTACW 1 cut(s) 903
TauI GCSGC 1 cut(s) 138
TfiI GAWTC 1 cut(s) 1105
Tru1I TTAA 4 cut(s) 411, 576, 642, 1122
Tru9I TTAA 4 cut(s) 411, 576, 642, 1122
TseFI GTSAC 5 cut(s) 86, 544, 677, 782, 992
TseI GCWGC 2 cut(s) 598, 607
Tsp45I GTSAC 5 cut(s) 86, 544, 677, 782, 992
TspDTI ATGAA 2 cut(s) 33, 335
TspGWI ACGGA 2 cut(s) 407, 816
TspMI CCCGGG 1 cut(s) 809
VneI GTGCAC 1 cut(s) 502
VpaK11BI GGWCC 3 cut(s) 304, 328, 806
XapI RAATTY 2 cut(s) 20, 298
XceI RCATGY 1 cut(s) 275
XhoI CTCGAG 1 cut(s) 107
XmaI CCCGGG 1 cut(s) 809
XmiI GTMKAC 1 cut(s) 219
XspI CTAG 1 cut(s) 696
ZraI GACGTC 1 cut(s) 28
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.