pycom08g10570

Sarcosine oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0013564
Physical Location & Seq
Forward (+)
8796823 .. 8797728
906 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g10570.1

Sequence Viewer

Length: 906 bp
ATGGAATATTCCGGCGATGAGTTCGACGTCATCGTAGTTGGAGCCGGCGTTATGGGAAGCTCCACCGCCTACCAGACCGCCAAGCGCGGCCAGAAGACCCTCCTCCTCGAGCAATTCGACTTCCTCCACCACCGCGGCTCCTCCCACGGCGAGTCCCGGACGATTCGCGCCACGTACCCGGAAGATTACTACACGCCCCTCGTCCTCGAGTCCTACAAGCTCTGGCAGCAGGCGGAGTCCGAAATCGGCTACAATGTCTACTTCAAAGCCAACCAATTGGACATGGCTCTGGCGAACGACAAGTTTCTCCTCGCCATTGTCGAGAGTTGTCGGAAAAACTCGGTCGCGTTTTCGGTCATGAACCGGGACCAGCTGCACCAGGAGTTCTCGGGTCGGGTTATGATTCCGGAGGATTGGGTGGGTGTTGTGACGGAGCACGGCGGGGTTATTAAACCCACCAAGGCGGTGTCAATGTTTCAAACGCTCGCTTTGCAGAACGGTGCCGTTTTGAGGGATAATATGGAGGTGAAGGGTGTGGAGAGAGATGGGGTGAGAGGAGGGGTTTGGGTATCCACAGCAAAAGGGGAGAGGTTTTGGGGGAAGAAATGTGTGGTGACGGTTGGGGCTTGGACGACAAAGTTAGTTAAAACGGTTGGCGGGATCGAACTGCCGATACAGCCGTTGGAGACCACCGTGTGCTACTGGCGGATTAAGGAGGGGCACGAGGGTGCTTTTGCTATCGGAGGAGACTTCCCCACCTTTGCTAGCTATGGGAACCCCTACATTTATGGGACGCCCTCTTTGGAGTATCCCGGTTTGATCGATCGAAGGTCGCCGTGCATGGCGGGTACCCGTGTGACCCCGATAAGAGGCCATGGGGTCCAGGGAACCCGCTGGCTCCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008115 GO:0008150 GO:0008152 GO:0016491 GO:0016645 GO:0016647 GO:0055114
KEGG Pathways
Metabolic & Signaling
ko00260 ko00310 ko01100 ko04146 map00260 map00310 map01100 map04146
Pfam Domains
Protein Families
DAO

Protein Analysis

302

Amino Acids

33.5

Weight (kDa)

7.69

Isoelectric Point (pI)

26.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 9 - 267 2.1e-35 FAD dependent oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013564)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24580
fragaria_vesca FvH4_2g36660
malus_domestica MD08G1126500.v1.1 MD15G1106400.v1.1
prunus_persica Prupe.1G458600_v2.0.a1 Prupe.1G458800_v2.0.a1 Prupe.1G458900_v2.0.a1
pyrus_communis pycom08g10570 pycom15g09710
rosa_chinensis RchiOBHm_Chr6g0305131
rosa_laevigata RLG00000010916
rosa_multiflora Rmu_sc0000061.1_g000020
rosa_rugosa Rorug06G0337900
rosa_samantha Rh6AG449500 Rh6BG451800 Rh6CG463000 Rh6DG450000
rosa_wichuraiana Rw6G039150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 30
Acc65I GGTACC 1 cut(s) 848
AccB1I GGYRCC 2 cut(s) 500, 848
AccI GTMKAC 1 cut(s) 258
AccII CGCG 4 cut(s) 87, 135, 168, 347
AccIII TCCGGA 1 cut(s) 406
AclWI GGATC 1 cut(s) 668
AcoI YGGCCR 1 cut(s) 88
AcyI GRCGYC 2 cut(s) 27, 794
AdeI CACNNNGTG 1 cut(s) 696
AfaI GTAC 2 cut(s) 176, 850
AfiI CCNNNNNNNGG 2 cut(s) 510, 869
AgsI TTSAA 2 cut(s) 265, 479
AjnI CCWGG 2 cut(s) 378, 882
AleI CACNNNNGTG 1 cut(s) 726
AluBI AGCT 4 cut(s) 60, 220, 373, 768
AluI AGCT 4 cut(s) 60, 220, 373, 768
Alw21I GWGCWC 1 cut(s) 438
Alw26I GTCTC 2 cut(s) 680, 741
AlwI GGATC 1 cut(s) 668
Ama87I CYCGRG 3 cut(s) 107, 206, 388
Aor13HI TCCGGA 1 cut(s) 406
AoxI GGCC 2 cut(s) 88, 871
ApeKI GCWGC 2 cut(s) 226, 373
ArsI GACNNNNNNTTYG 2 cut(s) 784, 816
Asp718I GGTACC 1 cut(s) 848
AspLEI GCGC 2 cut(s) 87, 170
AspS9I GGNCC 2 cut(s) 367, 880
AsuC2I CCSGG 4 cut(s) 157, 179, 365, 813
AsuHPI GGTGA 3 cut(s) 538, 562, 625
AsuNHI GCTAGC 1 cut(s) 764
AvaI CYCGRG 3 cut(s) 107, 206, 388
AvaII GGWCC 2 cut(s) 367, 880
BaeGI GKGCMC 1 cut(s) 723
BaeI ACNNNNGTAYC 4 cut(s) 665, 698, 840, 873
BanI GGYRCC 2 cut(s) 500, 848
BauI CACGAG 1 cut(s) 722
BbsI GAAGAC 1 cut(s) 101
Bbv12I GWGCWC 1 cut(s) 438
BbvI GCAGC 2 cut(s) 238, 360
BccI CCATC 1 cut(s) 539
BceAI ACGGC 5 cut(s) 163, 454, 488, 664, 820
BciT130I CCWGG 2 cut(s) 380, 884
BciVI GTATCC 2 cut(s) 580, 819
BcnI CCSGG 4 cut(s) 157, 179, 365, 813
BcoDI GTCTC 2 cut(s) 680, 741
BfaI CTAG 1 cut(s) 765
BfuI GTATCC 2 cut(s) 580, 819
BisI GCNGC 4 cut(s) 88, 136, 227, 374
BlsI GCNGC 4 cut(s) 89, 137, 228, 375
Bme1390I CCNGG 6 cut(s) 157, 179, 365, 380, 813, 884
Bme18I GGWCC 2 cut(s) 367, 880
BmeT110I CYCGRG 3 cut(s) 107, 206, 388
BmgT120I GGNCC 2 cut(s) 367, 880
BmiI GGNNCC 9 cut(s) 43, 139, 368, 502, 776, 850, 881, 889, 899
BmrFI CCNGG 6 cut(s) 157, 179, 365, 380, 813, 884
BmtI GCTAGC 1 cut(s) 768
BpiI GAAGAC 1 cut(s) 101
BpuMI CCSGG 4 cut(s) 157, 179, 365, 813
Bsa29I ATCGAT 1 cut(s) 822
BsaAI YACGTR 1 cut(s) 174
BsaHI GRCGYC 2 cut(s) 27, 794
BsaI GGTCTC 1 cut(s) 680
BsaJI CCNNGG 5 cut(s) 133, 145, 459, 874, 883
BsaWI WCCGGW 1 cut(s) 406
BsaXI ACNNNNNCTCC 4 cut(s) 33, 63, 122, 152
Bsc4I CCNNNNNNNGG 2 cut(s) 510, 869
Bse118I RCCGGY 1 cut(s) 44
Bse1I ACTGG 1 cut(s) 707
BseAI TCCGGA 1 cut(s) 406
BseBI CCWGG 2 cut(s) 380, 884
BseCI ATCGAT 1 cut(s) 822
BseDI CCNNGG 5 cut(s) 133, 145, 459, 874, 883
BseLI CCNNNNNNNGG 2 cut(s) 510, 869
BseNI ACTGG 1 cut(s) 707
BseRI GAGGAG 6 cut(s) 92, 95, 130, 299, 570, 759
BseSI GKGCMC 1 cut(s) 723
BseXI GCAGC 2 cut(s) 238, 360
BsgI GTGCAG 1 cut(s) 359
Bsh1236I CGCG 4 cut(s) 87, 135, 168, 347
Bsh1285I CGRYCG 2 cut(s) 345, 826
BshFI GGCC 2 cut(s) 90, 873
BshNI GGYRCC 2 cut(s) 500, 848
BshVI ATCGAT 1 cut(s) 822
BsiEI CGRYCG 2 cut(s) 345, 826
BsiHKAI GWGCWC 1 cut(s) 438
BsiHKCI CYCGRG 3 cut(s) 107, 206, 388
BsiSI CCGG 7 cut(s) 12, 45, 157, 179, 364, 407, 813
BslFI GGGAC 3 cut(s) 139, 380, 805
BslI CCNNNNNNNGG 2 cut(s) 510, 869
BsmAI GTCTC 2 cut(s) 680, 741
BsmFI GGGAC 3 cut(s) 139, 380, 805
BsnI GGCC 2 cut(s) 90, 873
Bso31I GGTCTC 1 cut(s) 680
BsoBI CYCGRG 3 cut(s) 107, 206, 388
Bsp1286I GDGCHC 2 cut(s) 438, 723
Bsp13I TCCGGA 1 cut(s) 406
Bsp143I GATC 3 cut(s) 660, 819, 823
Bsp19I CCATGG 1 cut(s) 874
BspANI GGCC 2 cut(s) 90, 873
BspDI ATCGAT 1 cut(s) 822
BspEI TCCGGA 1 cut(s) 406
BspFNI CGCG 4 cut(s) 87, 135, 168, 347
BspHI TCATGA 1 cut(s) 357
BspLI GGNNCC 9 cut(s) 43, 139, 368, 502, 776, 850, 881, 889, 899
BspOI GCTAGC 1 cut(s) 768
BspPI GGATC 1 cut(s) 668
BspT107I GGYRCC 2 cut(s) 500, 848
BspTNI GGTCTC 1 cut(s) 680
BsrFI RCCGGY 1 cut(s) 44
BsrI ACTGG 1 cut(s) 707
BssAI RCCGGY 1 cut(s) 44
BssECI CCNNGG 5 cut(s) 133, 145, 459, 874, 883
BssMI GATC 3 cut(s) 660, 819, 823
BssNI GRCGYC 2 cut(s) 27, 794
BssSI CACGAG 1 cut(s) 722
BssT1I CCWWGG 2 cut(s) 459, 874
Bst2BI CACGAG 1 cut(s) 722
Bst2UI CCWGG 2 cut(s) 380, 884
Bst4CI ACNGT 4 cut(s) 500, 619, 652, 694
BstACI GRCGYC 2 cut(s) 27, 794
BstBAI YACGTR 1 cut(s) 174
BstC8I GCNNGC 5 cut(s) 46, 231, 486, 766, 896
BstDSI CCRYGG 3 cut(s) 133, 145, 874
BstFNI CGCG 4 cut(s) 87, 135, 168, 347
BstHHI GCGC 2 cut(s) 87, 170
BstKTI GATC 3 cut(s) 663, 822, 826
BstMAI GTCTC 2 cut(s) 680, 741
BstMBI GATC 3 cut(s) 660, 819, 823
BstMCI CGRYCG 2 cut(s) 345, 826
BstMWI GCNNNNNNNGC 3 cut(s) 226, 490, 676
BstNI CCWGG 2 cut(s) 380, 884
BstSCI CCNGG 6 cut(s) 155, 177, 363, 378, 811, 882
BstSLI GKGCMC 1 cut(s) 723
BstUI CGCG 4 cut(s) 87, 135, 168, 347
BstV1I GCAGC 2 cut(s) 238, 360
BstV2I GAAGAC 1 cut(s) 101
BstXI CCANNNNNNTGG 1 cut(s) 277
Bsu15I ATCGAT 1 cut(s) 822
BsuI GTATCC 2 cut(s) 580, 819
BsuRI GGCC 2 cut(s) 90, 873
BsuTUI ATCGAT 1 cut(s) 822
BtgI CCRYGG 3 cut(s) 133, 145, 874
BtgZI GCGATG 1 cut(s) 30
Cac8I GCNNGC 5 cut(s) 46, 231, 486, 766, 896
CciI TCATGA 1 cut(s) 357
CfoI GCGC 2 cut(s) 87, 170
Cfr10I RCCGGY 1 cut(s) 44
Cfr13I GGNCC 2 cut(s) 367, 880
Cfr42I CCGCGG 1 cut(s) 136
ClaI ATCGAT 1 cut(s) 822
CseI GACGC 1 cut(s) 802
Csp6I GTAC 2 cut(s) 175, 849
CviAII CATG 4 cut(s) 283, 358, 841, 875
CviQI GTAC 2 cut(s) 175, 849
DpnI GATC 3 cut(s) 662, 821, 825
DpnII GATC 3 cut(s) 660, 819, 823
DraIII CACNNNGTG 1 cut(s) 696
EaeI YGGCCR 1 cut(s) 88
EciI GGCGGA 2 cut(s) 248, 721
Eco130I CCWWGG 2 cut(s) 459, 874
Eco31I GGTCTC 1 cut(s) 680
Eco47I GGWCC 2 cut(s) 367, 880
Eco88I CYCGRG 3 cut(s) 107, 206, 388
EcoRII CCWGG 2 cut(s) 378, 882
EcoT14I CCWWGG 2 cut(s) 459, 874
ErhI CCWWGG 2 cut(s) 459, 874
FaeI CATG 4 cut(s) 286, 361, 844, 878
FaiI YATR 9 cut(s) 53, 284, 359, 401, 521, 771, 789, 842, 876
FaqI GGGAC 3 cut(s) 139, 380, 805
FatI CATG 4 cut(s) 282, 357, 840, 874
FauI CCCGC 4 cut(s) 434, 650, 838, 899
FblI GTMKAC 1 cut(s) 258
Fnu4HI GCNGC 4 cut(s) 88, 136, 227, 374
Fsp4HI GCNGC 4 cut(s) 88, 136, 227, 374
FspBI CTAG 1 cut(s) 765
GlaI GCGC 2 cut(s) 86, 169
GluI GCNGC 4 cut(s) 88, 136, 227, 374
HaeIII GGCC 2 cut(s) 90, 873
HapII CCGG 7 cut(s) 12, 45, 157, 179, 364, 407, 813
HgaI GACGC 1 cut(s) 802
HhaI GCGC 2 cut(s) 87, 170
Hin1I GRCGYC 2 cut(s) 27, 794
Hin1II CATG 4 cut(s) 286, 361, 844, 878
Hin6I GCGC 2 cut(s) 85, 168
HinP1I GCGC 2 cut(s) 85, 168
HinfI GANTC 5 cut(s) 152, 163, 209, 236, 403
HpaII CCGG 7 cut(s) 12, 45, 157, 179, 364, 407, 813
HphI GGTGA 3 cut(s) 538, 562, 625
Hpy166II GTNNAC 1 cut(s) 259
Hpy188I TCNGA 3 cut(s) 241, 333, 743
Hpy188III TCNNGA 3 cut(s) 322, 358, 407
Hpy8I GTNNAC 1 cut(s) 259
Hpy99I CGWCG 1 cut(s) 29
HpyAV CCTTC 2 cut(s) 523, 822
HpyCH4III ACNGT 4 cut(s) 500, 619, 652, 694
HpyCH4IV ACGT 2 cut(s) 27, 173
HpyCH4V TGCA 3 cut(s) 376, 493, 840
HpyF10VI GCNNNNNNNGC 3 cut(s) 226, 490, 676
HpySE526I ACGT 2 cut(s) 27, 173
Hsp92I GRCGYC 2 cut(s) 27, 794
Hsp92II CATG 4 cut(s) 286, 361, 844, 878
HspAI GCGC 2 cut(s) 85, 168
Kpn2I TCCGGA 1 cut(s) 406
KpnI GGTACC 1 cut(s) 852
KroI GCCGGC 1 cut(s) 44
KroNI GCCGGC 1 cut(s) 46
KspI CCGCGG 1 cut(s) 136
Kzo9I GATC 3 cut(s) 660, 819, 823
LmnI GCTCC 5 cut(s) 41, 65, 143, 433, 903
Lsp1109I GCAGC 2 cut(s) 238, 360
MaeI CTAG 1 cut(s) 765
MaeII ACGT 2 cut(s) 27, 173
MaeIII GTNAC 3 cut(s) 427, 613, 856
MalI GATC 3 cut(s) 662, 821, 825
MboI GATC 3 cut(s) 660, 819, 823
MboII GAAGA 3 cut(s) 106, 194, 613
MfeI CAATTG 1 cut(s) 275
MhlI GDGCHC 2 cut(s) 438, 723
MluCI AATT 2 cut(s) 113, 275
MlyI GAGTC 3 cut(s) 161, 218, 245
MmeI TCCRAC 3 cut(s) 19, 311, 663
MroI TCCGGA 1 cut(s) 406
MroNI GCCGGC 1 cut(s) 44
MseI TTAA 3 cut(s) 450, 645, 711
MslI CAYNNNNRTG 1 cut(s) 726
MspA1I CMGCKG 3 cut(s) 135, 373, 894
MspI CCGG 7 cut(s) 12, 45, 157, 179, 364, 407, 813
MspR9I CCNGG 6 cut(s) 157, 179, 365, 380, 813, 884
MunI CAATTG 1 cut(s) 275
MvaI CCWGG 2 cut(s) 380, 884
MvnI CGCG 4 cut(s) 87, 135, 168, 347
MwoI GCNNNNNNNGC 3 cut(s) 226, 490, 676
NaeI GCCGGC 1 cut(s) 46
NciI CCSGG 4 cut(s) 157, 179, 365, 813
NcoI CCATGG 1 cut(s) 874
NdeII GATC 3 cut(s) 660, 819, 823
NgoMIV GCCGGC 1 cut(s) 44
NheI GCTAGC 1 cut(s) 764
NlaIII CATG 4 cut(s) 286, 361, 844, 878
NlaIV GGNNCC 9 cut(s) 43, 139, 368, 502, 776, 850, 881, 889, 899
NmuCI GTSAC 3 cut(s) 427, 613, 856
OliI CACNNNNGTG 1 cut(s) 726
PaeR7I CTCGAG 2 cut(s) 107, 206
PagI TCATGA 1 cut(s) 357
PcsI WCGNNNNNNNCGW 3 cut(s) 30, 114, 318
PdiI GCCGGC 1 cut(s) 46
PfeI GAWTC 2 cut(s) 163, 403
PfoI TCCNGGA 1 cut(s) 155
PkrI GCNGC 4 cut(s) 89, 137, 228, 375
Ple19I CGATCG 1 cut(s) 826
PleI GAGTC 3 cut(s) 160, 217, 244
PpsI GAGTC 3 cut(s) 160, 217, 244
Ppu21I YACGTR 1 cut(s) 174
Psp6I CCWGG 2 cut(s) 378, 882
PspGI CCWGG 2 cut(s) 378, 882
PspN4I GGNNCC 9 cut(s) 43, 139, 368, 502, 776, 850, 881, 889, 899
PspPI GGNCC 2 cut(s) 367, 880
PspXI VCTCGAGB 2 cut(s) 107, 206
PsrI GAACNNNNNNTAC 2 cut(s) 657, 689
PvuI CGATCG 1 cut(s) 826
PvuII CAGCTG 1 cut(s) 373
RsaI GTAC 2 cut(s) 176, 850
RsaNI GTAC 2 cut(s) 175, 849
RseI CAYNNNNRTG 1 cut(s) 726
SacII CCGCGG 1 cut(s) 136
SaqAI TTAA 3 cut(s) 450, 645, 711
SatI GCNGC 4 cut(s) 88, 136, 227, 374
Sau3AI GATC 3 cut(s) 660, 819, 823
Sau96I GGNCC 2 cut(s) 367, 880
SchI GAGTC 3 cut(s) 161, 218, 245
ScrFI CCNGG 6 cut(s) 157, 179, 365, 380, 813, 884
SduI GDGCHC 2 cut(s) 438, 723
Sfr274I CTCGAG 2 cut(s) 107, 206
Sfr303I CCGCGG 1 cut(s) 136
SgrBI CCGCGG 1 cut(s) 136
SinI GGWCC 2 cut(s) 367, 880
SlaI CTCGAG 2 cut(s) 107, 206
SmiMI CAYNNNNRTG 1 cut(s) 726
SmlI CTYRAG 2 cut(s) 107, 206
SmoI CTYRAG 2 cut(s) 107, 206
Sse9I AATT 2 cut(s) 113, 275
SspI AATATT 1 cut(s) 8
SspMI CTAG 1 cut(s) 765
StyD4I CCNGG 6 cut(s) 155, 177, 363, 378, 811, 882
StyI CCWWGG 2 cut(s) 459, 874
TaaI ACNGT 4 cut(s) 500, 619, 652, 694
TaiI ACGT 2 cut(s) 30, 176
TaqI TCGA 8 cut(s) 24, 108, 117, 207, 321, 663, 822, 826
TaqII GACCGA 2 cut(s) 331, 343
TasI AATT 2 cut(s) 113, 275
TauI GCSGC 2 cut(s) 90, 138
TfiI GAWTC 2 cut(s) 163, 403
Tru1I TTAA 3 cut(s) 450, 645, 711
Tru9I TTAA 3 cut(s) 450, 645, 711
TseFI GTSAC 3 cut(s) 427, 613, 856
TseI GCWGC 2 cut(s) 226, 373
Tsp45I GTSAC 3 cut(s) 427, 613, 856
TspDTI ATGAA 1 cut(s) 374
TspGWI ACGGA 2 cut(s) 446, 890
VpaK11BI GGWCC 2 cut(s) 367, 880
XhoI CTCGAG 2 cut(s) 107, 206
XmiI GTMKAC 1 cut(s) 258
XspI CTAG 1 cut(s) 765
ZraI GACGTC 1 cut(s) 28
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.