RchiOBHm_Chr6g0305131

Sarcosine oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0013564
Physical Location & Seq
Reverse (-)
64202910 .. 64204608
1699 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27421

Sequence Viewer

Length: 1155 bp
ATGGAATCTTGCGTCCACGAGTTCGACGTCATAGTCGTCGGGGCCGGAATTATGGGCAGCTCCACCGCCTACCAGACCGCCAAGAGGGGCCACAAGACCCTCCTGCTCGAGCAATTCGACTTCCTCCACCACCGGGGCTCGTCTCATGGCGAGTCCCGGACGATCCGGGCTACGTATCCAGAGGACTATTACGGTCCTCTGGTACTCGAGTCCTACAAGCTATGGCTACAAGCAGAGTCAGAGATCGGGTACAATGTCTATTTCAAGGCGCATCAGTTGGATATGGGCCAAGAAACCGACAGAGTTGTCCAGGCAGTTGTGGGTAGCTGTCGAAAGAACGAGCTTGGTCACCGGGTGCTGACCCGGGAGCAAGTGGAGCAGGAGTACTCGGGTCGGATCAAGATACCGGAGGGGTGGGTAGGTTTGGTTACGGAGCACGGTGGCGTGATCAAGCCCACCAAGGCGGTGTCGATGTTCCAAACTCTGGCCTTGCAAAACGGCGCCGTGCTGAGGGACAACATGGAGGTGACGGAGATTAAGAGAGATGAAGTGAGGGGAGGCATTTGGGTGGGGGTAGCAAATGGGGAGAGGTTTTGGGGCAAGAAATGTGTGGTCACAGTTGGGGCTTGGACTAAGAGGTTAGTTAAAACGGTTGGTGGGTTTGAATTGCCTATACAGCCCTTGGAGACCAGTGTGTGTTATTGGAGGATCAAGGAGGGTCATGAAGCTAGGTTTGCCATTGGAGGTGACTTCCCTACTTTTGCTAGCTATGGGGTTGCGGTGCATGGCGGGTACCCGTGTGACCCGGATAAGAGGCCGTGGGGACCCGGGAACCCGCTGGCTCCGTTGAAGGAGTGGATAGAGCAGACATTCTCCGGCGTGGTTGATTCCGGAGGGCCGGTTGCCACTCAGTTGTGTATGTATTCCATGACCCCAGATGAGGATTATGTGATTGATTTCATTGGAGGTGAGTTCGGGAAGGATGTGGTGATCGGAGGCGGGTTTTCAGGTCACGGGTTCAAGCTGTCTCCGGTGGTGGGGAGGATATTGGCAGACCTTGCACTTAGTGGACAGGCTAAAGGAGTGGAGCTAAAGCACTTTAGGATAGCAAGGTTTCAAGAGAATCCTCAAGGCAATCTTAAAGATTTCCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008115 GO:0008150 GO:0008152 GO:0016491 GO:0016645 GO:0016647 GO:0055114
KEGG Pathways
Metabolic & Signaling
ko00260 ko00310 ko01100 ko04146 map00260 map00310 map01100 map04146
Pfam Domains
Protein Families
DAO

Protein Analysis

384

Amino Acids

42.44

Weight (kDa)

6.2

Isoelectric Point (pI)

26.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 9 - 353 2.7e-44 FAD dependent oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013564)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24580
fragaria_vesca FvH4_2g36660
malus_domestica MD08G1126500.v1.1 MD15G1106400.v1.1
prunus_persica Prupe.1G458600_v2.0.a1 Prupe.1G458800_v2.0.a1 Prupe.1G458900_v2.0.a1
pyrus_communis pycom08g10570 pycom15g09710
rosa_chinensis RchiOBHm_Chr6g0305131
rosa_laevigata RLG00000010916
rosa_multiflora Rmu_sc0000061.1_g000020
rosa_rugosa Rorug06G0337900
rosa_samantha Rh6AG449500 Rh6BG451800 Rh6CG463000 Rh6DG450000
rosa_wichuraiana Rw6G039150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 32, 305
AatII GACGTC 1 cut(s) 30
Acc65I GGTACC 1 cut(s) 792
AccB1I GGYRCC 2 cut(s) 500, 792
AccB7I CCANNNNNTGG 1 cut(s) 484
AccIII TCCGGA 1 cut(s) 890
AciI CCGC 7 cut(s) 66, 78, 464, 779, 789, 836, 999
AclWI GGATC 3 cut(s) 157, 404, 716
AcyI GRCGYC 2 cut(s) 27, 501
AdeI CACNNNGTG 2 cut(s) 355, 1067
AfaI GTAC 4 cut(s) 204, 251, 386, 794
AfiI CCNNNNNNNGG 6 cut(s) 84, 133, 484, 510, 940, 1037
AgsI TTSAA 5 cut(s) 265, 665, 850, 1021, 1118
AjnI CCWGG 1 cut(s) 309
AluBI AGCT 8 cut(s) 60, 220, 327, 343, 728, 768, 1024, 1090
AluI AGCT 8 cut(s) 60, 220, 327, 343, 728, 768, 1024, 1090
Alw21I GWGCWC 1 cut(s) 438
Alw26I GTCTC 3 cut(s) 147, 680, 1032
AlwI GGATC 3 cut(s) 157, 404, 716
Ama87I CYCGRG 5 cut(s) 107, 206, 363, 388, 827
Aor13HI TCCGGA 1 cut(s) 890
AoxI GGCC 6 cut(s) 42, 88, 286, 486, 815, 896
ApeKI GCWGC 1 cut(s) 57
Asp718I GGTACC 1 cut(s) 792
AspLEI GCGC 2 cut(s) 271, 503
AspS9I GGNCC 6 cut(s) 42, 88, 194, 286, 824, 896
AsuC2I CCSGG 9 cut(s) 134, 157, 167, 353, 364, 365, 806, 828, 829
AsuHPI GGTGA 5 cut(s) 341, 538, 758, 980, 1000
AsuNHI GCTAGC 1 cut(s) 764
AvaI CYCGRG 5 cut(s) 107, 206, 363, 388, 827
AvaII GGWCC 2 cut(s) 194, 824
BaeI ACNNNNGTAYC 4 cut(s) 194, 227, 784, 817
BanI GGYRCC 2 cut(s) 500, 792
BanII GRGCYC 1 cut(s) 140
BauI CACGAG 1 cut(s) 17
Bbv12I GWGCWC 1 cut(s) 438
BbvCI CCTCAGC 1 cut(s) 509
BbvI GCAGC 1 cut(s) 69
BceAI ACGGC 3 cut(s) 488, 514, 802
BciT130I CCWGG 1 cut(s) 311
BciVI GTATCC 1 cut(s) 186
BclI TGATCA 1 cut(s) 447
BcnI CCSGG 9 cut(s) 134, 157, 167, 353, 364, 365, 806, 828, 829
BcoDI GTCTC 3 cut(s) 147, 680, 1032
BfaI CTAG 2 cut(s) 729, 765
BfoI RGCGCY 1 cut(s) 504
BfuI GTATCC 1 cut(s) 186
BisI GCNGC 1 cut(s) 58
BlsI GCNGC 1 cut(s) 59
BmcAI AGTACT 1 cut(s) 386
Bme18I GGWCC 2 cut(s) 194, 824
BmeT110I CYCGRG 5 cut(s) 107, 206, 363, 388, 827
BmgT120I GGNCC 6 cut(s) 42, 88, 194, 286, 824, 896
BmiI GGNNCC 8 cut(s) 43, 89, 502, 794, 825, 826, 833, 843
BmsI GCATC 1 cut(s) 280
BmtI GCTAGC 1 cut(s) 768
Bpu10I CCTNAGC 1 cut(s) 509
BpuEI CTTGAG 1 cut(s) 1113
BpuMI CCSGG 9 cut(s) 134, 157, 167, 353, 364, 365, 806, 828, 829
BsaAI YACGTR 1 cut(s) 174
BsaHI GRCGYC 2 cut(s) 27, 501
BsaI GGTCTC 1 cut(s) 680
BsaJI CCNNGG 6 cut(s) 133, 363, 459, 681, 818, 827
BsaWI WCCGGW 3 cut(s) 406, 890, 1030
BsaXI ACNNNNNCTCC 4 cut(s) 368, 398, 885, 915
Bsc4I CCNNNNNNNGG 6 cut(s) 84, 133, 484, 510, 940, 1037
Bse118I RCCGGY 1 cut(s) 898
Bse1I ACTGG 1 cut(s) 690
BseAI TCCGGA 1 cut(s) 890
BseBI CCWGG 1 cut(s) 311
BseDI CCNNGG 6 cut(s) 133, 363, 459, 681, 818, 827
BseGI GGATG 1 cut(s) 988
BseLI CCNNNNNNNGG 6 cut(s) 84, 133, 484, 510, 940, 1037
BseMII CTCAG 2 cut(s) 500, 923
BseNI ACTGG 1 cut(s) 690
BseXI GCAGC 1 cut(s) 69
BshFI GGCC 6 cut(s) 44, 90, 288, 488, 817, 898
BshNI GGYRCC 2 cut(s) 500, 792
BsiHKAI GWGCWC 1 cut(s) 438
BsiHKCI CYCGRG 5 cut(s) 107, 206, 363, 388, 827
BslFI GGGAC 3 cut(s) 139, 527, 837
BslI CCNNNNNNNGG 6 cut(s) 84, 133, 484, 510, 940, 1037
BsmAI GTCTC 3 cut(s) 147, 680, 1032
BsmBI CGTCTC 1 cut(s) 147
BsmFI GGGAC 3 cut(s) 139, 527, 837
BsnI GGCC 6 cut(s) 44, 90, 288, 488, 817, 898
Bso31I GGTCTC 1 cut(s) 680
BsoBI CYCGRG 5 cut(s) 107, 206, 363, 388, 827
Bsp1286I GDGCHC 2 cut(s) 140, 438
Bsp13I TCCGGA 1 cut(s) 890
Bsp143I GATC 6 cut(s) 162, 243, 396, 447, 708, 990
BspACI CCGC 7 cut(s) 66, 78, 464, 779, 789, 836, 999
BspANI GGCC 6 cut(s) 44, 90, 288, 488, 817, 898
BspCNI CTCAG 2 cut(s) 501, 922
BspEI TCCGGA 1 cut(s) 890
BspHI TCATGA 1 cut(s) 721
BspLI GGNNCC 8 cut(s) 43, 89, 502, 794, 825, 826, 833, 843
BspOI GCTAGC 1 cut(s) 768
BspPI GGATC 3 cut(s) 157, 404, 716
BspT107I GGYRCC 2 cut(s) 500, 792
BspTNI GGTCTC 1 cut(s) 680
BsrFI RCCGGY 1 cut(s) 898
BsrI ACTGG 1 cut(s) 690
BssAI RCCGGY 1 cut(s) 898
BssECI CCNNGG 6 cut(s) 133, 363, 459, 681, 818, 827
BssMI GATC 6 cut(s) 162, 243, 396, 447, 708, 990
BssNI GRCGYC 2 cut(s) 27, 501
BssSI CACGAG 1 cut(s) 17
BssT1I CCWWGG 2 cut(s) 459, 681
Bst2BI CACGAG 1 cut(s) 17
Bst2UI CCWGG 1 cut(s) 311
Bst4CI ACNGT 4 cut(s) 194, 440, 619, 652
BstACI GRCGYC 2 cut(s) 27, 501
BstAPI GCANNNNNTGC 1 cut(s) 1058
BstBAI YACGTR 1 cut(s) 174
BstC8I GCNNGC 2 cut(s) 766, 840
BstDEI CTNAG 4 cut(s) 509, 633, 909, 1064
BstDSI CCRYGG 1 cut(s) 818
BstEII GGTNACC 1 cut(s) 347
BstF5I GGATG 1 cut(s) 988
BstH2I RGCGCY 1 cut(s) 504
BstHHI GCGC 2 cut(s) 271, 503
BstKTI GATC 6 cut(s) 165, 246, 399, 450, 711, 993
BstMAI GTCTC 3 cut(s) 147, 680, 1032
BstMBI GATC 6 cut(s) 162, 243, 396, 447, 708, 990
BstMWI GCNNNNNNNGC 4 cut(s) 376, 676, 734, 1058
BstNI CCWGG 1 cut(s) 311
BstPI GGTNACC 1 cut(s) 347
BstSNI TACGTA 1 cut(s) 174
BstV1I GCAGC 1 cut(s) 69
BsuI GTATCC 1 cut(s) 186
BsuRI GGCC 6 cut(s) 44, 90, 288, 488, 817, 898
BtgI CCRYGG 1 cut(s) 818
BtsCI GGATG 1 cut(s) 988
BtsIMutI CAGTG 1 cut(s) 697
Cac8I GCNNGC 2 cut(s) 766, 840
CciI TCATGA 1 cut(s) 721
CfoI GCGC 2 cut(s) 271, 503
Cfr10I RCCGGY 1 cut(s) 898
Cfr13I GGNCC 6 cut(s) 42, 88, 194, 286, 824, 896
Cfr9I CCCGGG 2 cut(s) 363, 827
Csp6I GTAC 4 cut(s) 203, 250, 385, 793
CviAII CATG 5 cut(s) 146, 520, 722, 785, 928
CviQI GTAC 4 cut(s) 203, 250, 385, 793
DdeI CTNAG 4 cut(s) 509, 633, 909, 1064
DinI GGCGCC 1 cut(s) 502
DpnI GATC 6 cut(s) 164, 245, 398, 449, 710, 992
DpnII GATC 6 cut(s) 162, 243, 396, 447, 708, 990
DraIII CACNNNGTG 2 cut(s) 355, 1067
DrdI GACNNNNNNGTC 2 cut(s) 32, 305
DseDI GACNNNNNNGTC 2 cut(s) 32, 305
Eco105I TACGTA 1 cut(s) 174
Eco130I CCWWGG 2 cut(s) 459, 681
Eco24I GRGCYC 1 cut(s) 140
Eco31I GGTCTC 1 cut(s) 680
Eco47I GGWCC 2 cut(s) 194, 824
Eco88I CYCGRG 5 cut(s) 107, 206, 363, 388, 827
Eco91I GGTNACC 1 cut(s) 347
EcoO109I RGGNCCY 1 cut(s) 824
EcoO65I GGTNACC 1 cut(s) 347
EcoRII CCWGG 1 cut(s) 309
EcoT14I CCWWGG 2 cut(s) 459, 681
EcoT38I GRGCYC 1 cut(s) 140
EgeI GGCGCC 1 cut(s) 502
EheI GGCGCC 1 cut(s) 502
ErhI CCWWGG 2 cut(s) 459, 681
Esp3I CGTCTC 1 cut(s) 147
FaeI CATG 5 cut(s) 149, 523, 725, 788, 931
FalI AAGNNNNNCTT 2 cut(s) 1122, 1154
FaqI GGGAC 3 cut(s) 139, 527, 837
FatI CATG 5 cut(s) 145, 519, 721, 784, 927
FauI CCCGC 3 cut(s) 782, 843, 992
FbaI TGATCA 1 cut(s) 447
Fnu4HI GCNGC 1 cut(s) 58
FokI GGATG 1 cut(s) 995
FriOI GRGCYC 1 cut(s) 140
Fsp4HI GCNGC 1 cut(s) 58
FspBI CTAG 2 cut(s) 729, 765
GlaI GCGC 2 cut(s) 270, 502
GluI GCNGC 1 cut(s) 58
HaeII RGCGCY 1 cut(s) 504
HaeIII GGCC 6 cut(s) 44, 90, 288, 488, 817, 898
HhaI GCGC 2 cut(s) 271, 503
Hin1I GRCGYC 2 cut(s) 27, 501
Hin1II CATG 5 cut(s) 149, 523, 725, 788, 931
Hin6I GCGC 2 cut(s) 269, 501
HinP1I GCGC 2 cut(s) 269, 501
HinfI GANTC 6 cut(s) 5, 152, 209, 236, 887, 1123
HphI GGTGA 5 cut(s) 341, 538, 758, 980, 1000
Hpy166II GTNNAC 2 cut(s) 16, 1070
Hpy188I TCNGA 3 cut(s) 241, 396, 995
Hpy188III TCNNGA 6 cut(s) 179, 400, 722, 891, 976, 1118
Hpy8I GTNNAC 2 cut(s) 16, 1070
Hpy99I CGWCG 2 cut(s) 29, 41
HpyAV CCTTC 2 cut(s) 844, 973
HpyCH4III ACNGT 4 cut(s) 194, 440, 619, 652
HpyCH4IV ACGT 2 cut(s) 27, 173
HpyCH4V TGCA 3 cut(s) 493, 784, 1061
HpyF10VI GCNNNNNNNGC 4 cut(s) 376, 676, 734, 1058
HpyF3I CTNAG 4 cut(s) 509, 633, 909, 1064
HpySE526I ACGT 2 cut(s) 27, 173
Hsp92I GRCGYC 2 cut(s) 27, 501
Hsp92II CATG 5 cut(s) 149, 523, 725, 788, 931
HspAI GCGC 2 cut(s) 269, 501
KasI GGCGCC 1 cut(s) 500
KflI GGGWCCC 1 cut(s) 824
Kpn2I TCCGGA 1 cut(s) 890
KpnI GGTACC 1 cut(s) 796
Ksp22I TGATCA 1 cut(s) 447
Kzo9I GATC 6 cut(s) 162, 243, 396, 447, 708, 990
LmnI GCTCC 6 cut(s) 65, 367, 376, 433, 847, 1087
Lsp1109I GCAGC 1 cut(s) 69
LweI GCATC 1 cut(s) 280
MaeI CTAG 2 cut(s) 729, 765
MaeII ACGT 2 cut(s) 27, 173
MaeIII GTNAC 7 cut(s) 347, 427, 526, 613, 746, 800, 1010
MalI GATC 6 cut(s) 164, 245, 398, 449, 710, 992
MboI GATC 6 cut(s) 162, 243, 396, 447, 708, 990
MhlI GDGCHC 2 cut(s) 140, 438
MluCI AATT 3 cut(s) 48, 113, 665
Mly113I GGCGCC 1 cut(s) 501
MlyI GAGTC 3 cut(s) 161, 218, 245
MmeI TCCRAC 2 cut(s) 258, 374
MroI TCCGGA 1 cut(s) 890
MseI TTAA 3 cut(s) 537, 645, 1140
MslI CAYNNNNRTG 2 cut(s) 524, 566
MspA1I CMGCKG 1 cut(s) 838
MvaI CCWGG 1 cut(s) 311
MwoI GCNNNNNNNGC 4 cut(s) 376, 676, 734, 1058
NarI GGCGCC 1 cut(s) 501
NciI CCSGG 9 cut(s) 134, 157, 167, 353, 364, 365, 806, 828, 829
NdeII GATC 6 cut(s) 162, 243, 396, 447, 708, 990
NheI GCTAGC 1 cut(s) 764
NlaIII CATG 5 cut(s) 149, 523, 725, 788, 931
NlaIV GGNNCC 8 cut(s) 43, 89, 502, 794, 825, 826, 833, 843
NmuCI GTSAC 6 cut(s) 347, 526, 613, 746, 800, 1010
PaeR7I CTCGAG 2 cut(s) 107, 206
PagI TCATGA 1 cut(s) 721
PcsI WCGNNNNNNNCGW 3 cut(s) 24, 33, 114
PfeI GAWTC 3 cut(s) 5, 887, 1123
PflMI CCANNNNNTGG 1 cut(s) 484
PfoI TCCNGGA 1 cut(s) 155
PkrI GCNGC 1 cut(s) 59
PleI GAGTC 3 cut(s) 160, 217, 244
PluTI GGCGCC 1 cut(s) 504
PpsI GAGTC 3 cut(s) 160, 217, 244
Ppu21I YACGTR 1 cut(s) 174
PpuMI RGGWCCY 1 cut(s) 824
Psp5II RGGWCCY 1 cut(s) 824
Psp6I CCWGG 1 cut(s) 309
PspEI GGTNACC 1 cut(s) 347
PspGI CCWGG 1 cut(s) 309
PspN4I GGNNCC 8 cut(s) 43, 89, 502, 794, 825, 826, 833, 843
PspPI GGNCC 6 cut(s) 42, 88, 194, 286, 824, 896
PspPPI RGGWCCY 1 cut(s) 824
PspXI VCTCGAGB 2 cut(s) 107, 206
RsaI GTAC 4 cut(s) 204, 251, 386, 794
RsaNI GTAC 4 cut(s) 203, 250, 385, 793
RseI CAYNNNNRTG 2 cut(s) 524, 566
SaqAI TTAA 3 cut(s) 537, 645, 1140
SatI GCNGC 1 cut(s) 58
Sau3AI GATC 6 cut(s) 162, 243, 396, 447, 708, 990
Sau96I GGNCC 6 cut(s) 42, 88, 194, 286, 824, 896
ScaI AGTACT 1 cut(s) 386
SchI GAGTC 3 cut(s) 161, 218, 245
SduI GDGCHC 2 cut(s) 140, 438
SfaNI GCATC 1 cut(s) 280
SfoI GGCGCC 1 cut(s) 502
Sfr274I CTCGAG 2 cut(s) 107, 206
SinI GGWCC 2 cut(s) 194, 824
SlaI CTCGAG 2 cut(s) 107, 206
SmaI CCCGGG 2 cut(s) 365, 829
SmiMI CAYNNNNRTG 2 cut(s) 524, 566
SmlI CTYRAG 3 cut(s) 107, 206, 1128
SmoI CTYRAG 3 cut(s) 107, 206, 1128
SnaBI TACGTA 1 cut(s) 174
Sse9I AATT 3 cut(s) 48, 113, 665
SsiI CCGC 7 cut(s) 66, 78, 464, 779, 789, 836, 999
SspDI GGCGCC 1 cut(s) 500
SspMI CTAG 2 cut(s) 729, 765
StyI CCWWGG 2 cut(s) 459, 681
TaaI ACNGT 4 cut(s) 194, 440, 619, 652
TaiI ACGT 2 cut(s) 30, 176
TaqI TCGA 6 cut(s) 24, 108, 117, 207, 331, 470
TasI AATT 3 cut(s) 48, 113, 665
TatI WGTACW 1 cut(s) 384
TfiI GAWTC 3 cut(s) 5, 887, 1123
Tru1I TTAA 3 cut(s) 537, 645, 1140
Tru9I TTAA 3 cut(s) 537, 645, 1140
TscAI CASTG 1 cut(s) 697
TseFI GTSAC 6 cut(s) 347, 526, 613, 746, 800, 1010
TseI GCWGC 1 cut(s) 57
Tsp45I GTSAC 6 cut(s) 347, 526, 613, 746, 800, 1010
TspDTI ATGAA 3 cut(s) 561, 738, 949
TspGWI ACGGA 3 cut(s) 446, 545, 834
TspMI CCCGGG 2 cut(s) 363, 827
TspRI CASTG 1 cut(s) 697
Van91I CCANNNNNTGG 1 cut(s) 484
VpaK11BI GGWCC 2 cut(s) 194, 824
XhoI CTCGAG 2 cut(s) 107, 206
XmaI CCCGGG 2 cut(s) 363, 827
XspI CTAG 2 cut(s) 729, 765
ZraI GACGTC 1 cut(s) 28
ZrmI AGTACT 1 cut(s) 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.