Rw6G039150

Sarcosine oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0013564
Physical Location & Seq
Reverse (-)
63175308 .. 63176513
1206 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G039150.1

Sequence Viewer

Length: 1206 bp
ATGGAATCTTGCGTCCACGAGTTCGACGTCATAGTCGTCGGTGCCGGAATTATGGGCAGCTCCACCGCCTACCAGACCGCCAAGAGGGGCCACAAGACCCTCCTGCTCGAGCAATTCGACTTCCTCCACCACCGGGGCTCGTCTCATGGCGAGTCCCGGACGATCCGGGCTACGTATCCTGAGGACTATTATGGTCCTCTGGTACTCGAGTCCTACAAGCTATGGCTACAAGCAGAGTCAGAGATCGGGTACAATGTCTATTTCAAGGCGCATCAGTTGGATATGGGCCAAGAAACCGACAGAGTTGTCCAGGCAGTTGTGGGTAGCTGTCGAAAGAACGAGCTTGGTCACCGGGTGCTGACCCGGGAGCAAGTGGAGCAGGAGTACTCGGGTCGGATCAAGATACCGGAGGGGTGGGTGGGTTTGGTTACGGAGCACGGTGGCGTGATCAAGCCCACCAAGGCCGTGTCGATGTTCCAAACTCTGGCCTTGCAAAACGGGGCCGTGCTGAGGGACAACATGGAGGTGACGGAGATTAAGAGAGATGAAGTGAGAGGAGGCATTTGGGTAGGGGTAGCAAATGGGGAGAGGTTTTGGGGTAAGAAATGTGTGGTCACAGTTGGGGCTTGGACTAAGAGGTTAGTTAAAACGGTTGGTGGGTTTGAATTGCCTGTACAGCCCTTGGAGACCAGTGTGTGTTATTGGAGGATCAAGGAGGGTCATGAAGCTAGGTTTGCCATTGGAGGTGACTTCCCTACTTTTGCTAGCTATGGGGTTCCATATATCTATGGTACCCCCTCATTGGAGTATCCGGGTTTGATCAAGGTTGCGGTGCATGGCGGGTACCCGTGTGACCAGGATAAGAGGCCGTGGGGACCCGGGAACCCGCTGGCTCCTTTGAAGGAGTGGATAGAGCAGACATTCTCCGGCGTGGTTGATTCCGGAGGGCCCGTTGCCACTCAGTTGTGTATGTATTCCATGACCCCAGATGAGGATTATGTGATTGATTTCATTGGAGGTGAGTTTGGGAAGGATGTGGTGATCGGAGGCGGGTTTTCAGGTCACGGGTTCAAGCTGTCTCCGGTGGTGGGGAGGATATTGGCAGACCTTGCACTTAGTGGAGAGGCTAAAGGAGTGGAGCTAAAGCACTTTAGGATAGCAAGGTTTCTTGAGAATCCTCAAGGCAATGTTAAAGATTTCCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008115 GO:0008150 GO:0008152 GO:0016491 GO:0016645 GO:0016647 GO:0055114
KEGG Pathways
Metabolic & Signaling
ko00260 ko00310 ko01100 ko04146 map00260 map00310 map01100 map04146
Pfam Domains
Protein Families
DAO

Protein Analysis

401

Amino Acids

44.32

Weight (kDa)

6.09

Isoelectric Point (pI)

26.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 9 - 370 2.9e-46 FAD dependent oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013564)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24580
fragaria_vesca FvH4_2g36660
malus_domestica MD08G1126500.v1.1 MD15G1106400.v1.1
prunus_persica Prupe.1G458600_v2.0.a1 Prupe.1G458800_v2.0.a1 Prupe.1G458900_v2.0.a1
pyrus_communis pycom08g10570 pycom15g09710
rosa_chinensis RchiOBHm_Chr6g0305131
rosa_laevigata RLG00000010916
rosa_multiflora Rmu_sc0000061.1_g000020
rosa_rugosa Rorug06G0337900
rosa_samantha Rh6AG449500 Rh6BG451800 Rh6CG463000 Rh6DG450000
rosa_wichuraiana Rw6G039150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 32, 305
AatII GACGTC 1 cut(s) 30
Acc65I GGTACC 2 cut(s) 791, 843
AccB1I GGYRCC 3 cut(s) 41, 791, 843
AccB7I CCANNNNNTGG 1 cut(s) 484
AccIII TCCGGA 1 cut(s) 941
AciI CCGC 6 cut(s) 66, 78, 830, 840, 887, 1050
AclWI GGATC 3 cut(s) 157, 404, 716
AcyI GRCGYC 1 cut(s) 27
AdeI CACNNNGTG 2 cut(s) 355, 1118
AfaI GTAC 6 cut(s) 204, 251, 386, 675, 793, 845
AfiI CCNNNNNNNGG 7 cut(s) 84, 133, 484, 510, 802, 991, 1088
AgsI TTSAA 4 cut(s) 265, 665, 901, 1072
AjnI CCWGG 2 cut(s) 309, 855
AluBI AGCT 8 cut(s) 60, 220, 327, 343, 728, 768, 1075, 1141
AluI AGCT 8 cut(s) 60, 220, 327, 343, 728, 768, 1075, 1141
Alw21I GWGCWC 1 cut(s) 438
Alw26I GTCTC 3 cut(s) 147, 680, 1083
AlwI GGATC 3 cut(s) 157, 404, 716
Ama87I CYCGRG 5 cut(s) 107, 206, 363, 388, 878
Aor13HI TCCGGA 1 cut(s) 941
AoxI GGCC 7 cut(s) 88, 286, 462, 486, 501, 866, 947
ApaI GGGCCC 1 cut(s) 951
ApeKI GCWGC 1 cut(s) 57
Asp718I GGTACC 2 cut(s) 791, 843
AspLEI GCGC 1 cut(s) 271
AspS9I GGNCC 7 cut(s) 88, 194, 286, 501, 875, 947, 948
AsuC2I CCSGG 9 cut(s) 134, 157, 167, 353, 364, 365, 813, 879, 880
AsuHPI GGTGA 5 cut(s) 341, 538, 758, 1031, 1051
AsuNHI GCTAGC 1 cut(s) 764
AvaI CYCGRG 5 cut(s) 107, 206, 363, 388, 878
AvaII GGWCC 2 cut(s) 194, 875
AxyI CCTNAGG 1 cut(s) 180
BaeGI GKGCMC 1 cut(s) 951
BaeI ACNNNNGTAYC 4 cut(s) 194, 227, 835, 868
BanI GGYRCC 3 cut(s) 41, 791, 843
BanII GRGCYC 2 cut(s) 140, 951
BauI CACGAG 1 cut(s) 17
Bbv12I GWGCWC 1 cut(s) 438
BbvCI CCTCAGC 1 cut(s) 509
BbvI GCAGC 1 cut(s) 69
BceAI ACGGC 3 cut(s) 449, 488, 853
BciT130I CCWGG 2 cut(s) 311, 857
BciVI GTATCC 2 cut(s) 186, 819
BclI TGATCA 2 cut(s) 447, 819
BcnI CCSGG 9 cut(s) 134, 157, 167, 353, 364, 365, 813, 879, 880
BcoDI GTCTC 3 cut(s) 147, 680, 1083
BfaI CTAG 2 cut(s) 729, 765
BfuI GTATCC 2 cut(s) 186, 819
BisI GCNGC 1 cut(s) 58
BlsI GCNGC 1 cut(s) 59
BmcAI AGTACT 1 cut(s) 386
Bme18I GGWCC 2 cut(s) 194, 875
BmeT110I CYCGRG 5 cut(s) 107, 206, 363, 388, 878
BmgT120I GGNCC 7 cut(s) 88, 194, 286, 501, 875, 947, 948
BmsI GCATC 1 cut(s) 280
BmtI GCTAGC 1 cut(s) 768
Bpu10I CCTNAGC 1 cut(s) 509
BpuEI CTTGAG 2 cut(s) 1164, 1190
BpuMI CCSGG 9 cut(s) 134, 157, 167, 353, 364, 365, 813, 879, 880
BsaAI YACGTR 1 cut(s) 174
BsaHI GRCGYC 1 cut(s) 27
BsaI GGTCTC 1 cut(s) 680
BsaJI CCNNGG 6 cut(s) 133, 363, 459, 681, 869, 878
BsaWI WCCGGW 3 cut(s) 406, 941, 1081
BsaXI ACNNNNNCTCC 4 cut(s) 368, 398, 936, 966
Bsc4I CCNNNNNNNGG 7 cut(s) 84, 133, 484, 510, 802, 991, 1088
Bse1I ACTGG 1 cut(s) 690
Bse21I CCTNAGG 1 cut(s) 180
Bse3DI GCAATG 1 cut(s) 1192
BseAI TCCGGA 1 cut(s) 941
BseBI CCWGG 2 cut(s) 311, 857
BseDI CCNNGG 6 cut(s) 133, 363, 459, 681, 869, 878
BseGI GGATG 1 cut(s) 1039
BseLI CCNNNNNNNGG 7 cut(s) 84, 133, 484, 510, 802, 991, 1088
BseMI GCAATG 1 cut(s) 1192
BseMII CTCAG 3 cut(s) 171, 500, 974
BseNI ACTGG 1 cut(s) 690
BseRI GAGGAG 1 cut(s) 570
BseSI GKGCMC 1 cut(s) 951
BseXI GCAGC 1 cut(s) 69
BshFI GGCC 7 cut(s) 90, 288, 464, 488, 503, 868, 949
BshNI GGYRCC 3 cut(s) 41, 791, 843
BsiHKAI GWGCWC 1 cut(s) 438
BsiHKCI CYCGRG 5 cut(s) 107, 206, 363, 388, 878
BslFI GGGAC 3 cut(s) 139, 527, 888
BslI CCNNNNNNNGG 7 cut(s) 84, 133, 484, 510, 802, 991, 1088
BsmAI GTCTC 3 cut(s) 147, 680, 1083
BsmBI CGTCTC 1 cut(s) 147
BsmFI GGGAC 3 cut(s) 139, 527, 888
BsnI GGCC 7 cut(s) 90, 288, 464, 488, 503, 868, 949
Bso31I GGTCTC 1 cut(s) 680
BsoBI CYCGRG 5 cut(s) 107, 206, 363, 388, 878
Bsp120I GGGCCC 1 cut(s) 947
Bsp1286I GDGCHC 3 cut(s) 140, 438, 951
Bsp13I TCCGGA 1 cut(s) 941
Bsp1407I TGTACA 1 cut(s) 673
Bsp143I GATC 7 cut(s) 162, 243, 396, 447, 708, 819, 1041
BspACI CCGC 6 cut(s) 66, 78, 830, 840, 887, 1050
BspANI GGCC 7 cut(s) 90, 288, 464, 488, 503, 868, 949
BspCNI CTCAG 3 cut(s) 172, 501, 973
BspEI TCCGGA 1 cut(s) 941
BspHI TCATGA 1 cut(s) 721
BspOI GCTAGC 1 cut(s) 768
BspPI GGATC 3 cut(s) 157, 404, 716
BspT107I GGYRCC 3 cut(s) 41, 791, 843
BspTNI GGTCTC 1 cut(s) 680
BsrDI GCAATG 1 cut(s) 1192
BsrGI TGTACA 1 cut(s) 673
BsrI ACTGG 1 cut(s) 690
BssECI CCNNGG 6 cut(s) 133, 363, 459, 681, 869, 878
BssMI GATC 7 cut(s) 162, 243, 396, 447, 708, 819, 1041
BssNI GRCGYC 1 cut(s) 27
BssSI CACGAG 1 cut(s) 17
BssT1I CCWWGG 2 cut(s) 459, 681
Bst2BI CACGAG 1 cut(s) 17
Bst2UI CCWGG 2 cut(s) 311, 857
Bst4CI ACNGT 3 cut(s) 440, 619, 652
BstACI GRCGYC 1 cut(s) 27
BstAPI GCANNNNNTGC 1 cut(s) 1109
BstAUI TGTACA 1 cut(s) 673
BstBAI YACGTR 1 cut(s) 174
BstC8I GCNNGC 2 cut(s) 766, 891
BstDEI CTNAG 5 cut(s) 180, 509, 633, 960, 1115
BstDSI CCRYGG 1 cut(s) 869
BstEII GGTNACC 1 cut(s) 347
BstF5I GGATG 1 cut(s) 1039
BstHHI GCGC 1 cut(s) 271
BstKTI GATC 7 cut(s) 165, 246, 399, 450, 711, 822, 1044
BstMAI GTCTC 3 cut(s) 147, 680, 1083
BstMBI GATC 7 cut(s) 162, 243, 396, 447, 708, 819, 1041
BstMWI GCNNNNNNNGC 4 cut(s) 376, 676, 734, 1109
BstNI CCWGG 2 cut(s) 311, 857
BstPI GGTNACC 1 cut(s) 347
BstSLI GKGCMC 1 cut(s) 951
BstSNI TACGTA 1 cut(s) 174
BstV1I GCAGC 1 cut(s) 69
Bsu36I CCTNAGG 1 cut(s) 180
BsuI GTATCC 2 cut(s) 186, 819
BsuRI GGCC 7 cut(s) 90, 288, 464, 488, 503, 868, 949
BtgI CCRYGG 1 cut(s) 869
BtsCI GGATG 1 cut(s) 1039
BtsIMutI CAGTG 1 cut(s) 697
Cac8I GCNNGC 2 cut(s) 766, 891
CciI TCATGA 1 cut(s) 721
CfoI GCGC 1 cut(s) 271
Cfr13I GGNCC 7 cut(s) 88, 194, 286, 501, 875, 947, 948
Cfr9I CCCGGG 2 cut(s) 363, 878
Csp6I GTAC 6 cut(s) 203, 250, 385, 674, 792, 844
CviAII CATG 5 cut(s) 146, 520, 722, 836, 979
CviQI GTAC 6 cut(s) 203, 250, 385, 674, 792, 844
DdeI CTNAG 5 cut(s) 180, 509, 633, 960, 1115
DpnI GATC 7 cut(s) 164, 245, 398, 449, 710, 821, 1043
DpnII GATC 7 cut(s) 162, 243, 396, 447, 708, 819, 1041
DraIII CACNNNGTG 2 cut(s) 355, 1118
DrdI GACNNNNNNGTC 2 cut(s) 32, 305
DseDI GACNNNNNNGTC 2 cut(s) 32, 305
Eco105I TACGTA 1 cut(s) 174
Eco130I CCWWGG 2 cut(s) 459, 681
Eco24I GRGCYC 2 cut(s) 140, 951
Eco31I GGTCTC 1 cut(s) 680
Eco47I GGWCC 2 cut(s) 194, 875
Eco81I CCTNAGG 1 cut(s) 180
Eco88I CYCGRG 5 cut(s) 107, 206, 363, 388, 878
Eco91I GGTNACC 1 cut(s) 347
EcoO109I RGGNCCY 2 cut(s) 875, 947
EcoO65I GGTNACC 1 cut(s) 347
EcoRII CCWGG 2 cut(s) 309, 855
EcoT14I CCWWGG 2 cut(s) 459, 681
EcoT38I GRGCYC 2 cut(s) 140, 951
ErhI CCWWGG 2 cut(s) 459, 681
Esp3I CGTCTC 1 cut(s) 147
FaeI CATG 5 cut(s) 149, 523, 725, 839, 982
FaqI GGGAC 3 cut(s) 139, 527, 888
FatI CATG 5 cut(s) 145, 519, 721, 835, 978
FauI CCCGC 3 cut(s) 833, 894, 1043
FbaI TGATCA 2 cut(s) 447, 819
Fnu4HI GCNGC 1 cut(s) 58
FokI GGATG 1 cut(s) 1046
FriOI GRGCYC 2 cut(s) 140, 951
Fsp4HI GCNGC 1 cut(s) 58
FspBI CTAG 2 cut(s) 729, 765
GlaI GCGC 1 cut(s) 270
GluI GCNGC 1 cut(s) 58
HaeIII GGCC 7 cut(s) 90, 288, 464, 488, 503, 868, 949
HhaI GCGC 1 cut(s) 271
Hin1I GRCGYC 1 cut(s) 27
Hin1II CATG 5 cut(s) 149, 523, 725, 839, 982
Hin6I GCGC 1 cut(s) 269
HinP1I GCGC 1 cut(s) 269
HinfI GANTC 6 cut(s) 5, 152, 209, 236, 938, 1174
HphI GGTGA 5 cut(s) 341, 538, 758, 1031, 1051
Hpy166II GTNNAC 1 cut(s) 16
Hpy188I TCNGA 3 cut(s) 241, 396, 1046
Hpy188III TCNNGA 5 cut(s) 179, 400, 722, 942, 1169
Hpy8I GTNNAC 1 cut(s) 16
Hpy99I CGWCG 2 cut(s) 29, 41
HpyAV CCTTC 2 cut(s) 895, 1024
HpyCH4III ACNGT 3 cut(s) 440, 619, 652
HpyCH4IV ACGT 2 cut(s) 27, 173
HpyCH4V TGCA 3 cut(s) 493, 835, 1112
HpyF10VI GCNNNNNNNGC 4 cut(s) 376, 676, 734, 1109
HpyF3I CTNAG 5 cut(s) 180, 509, 633, 960, 1115
HpySE526I ACGT 2 cut(s) 27, 173
Hsp92I GRCGYC 1 cut(s) 27
Hsp92II CATG 5 cut(s) 149, 523, 725, 839, 982
HspAI GCGC 1 cut(s) 269
KflI GGGWCCC 1 cut(s) 875
Kpn2I TCCGGA 1 cut(s) 941
KpnI GGTACC 2 cut(s) 795, 847
Ksp22I TGATCA 2 cut(s) 447, 819
Kzo9I GATC 7 cut(s) 162, 243, 396, 447, 708, 819, 1041
LmnI GCTCC 6 cut(s) 65, 367, 376, 433, 898, 1138
Lsp1109I GCAGC 1 cut(s) 69
LweI GCATC 1 cut(s) 280
MaeI CTAG 2 cut(s) 729, 765
MaeII ACGT 2 cut(s) 27, 173
MaeIII GTNAC 7 cut(s) 347, 427, 526, 613, 746, 851, 1061
MalI GATC 7 cut(s) 164, 245, 398, 449, 710, 821, 1043
MboI GATC 7 cut(s) 162, 243, 396, 447, 708, 819, 1041
MhlI GDGCHC 3 cut(s) 140, 438, 951
MluCI AATT 3 cut(s) 48, 113, 665
MlyI GAGTC 3 cut(s) 161, 218, 245
MmeI TCCRAC 2 cut(s) 258, 374
MroI TCCGGA 1 cut(s) 941
MseI TTAA 3 cut(s) 537, 645, 1191
MslI CAYNNNNRTG 1 cut(s) 524
MspA1I CMGCKG 1 cut(s) 889
MvaI CCWGG 2 cut(s) 311, 857
MwoI GCNNNNNNNGC 4 cut(s) 376, 676, 734, 1109
NciI CCSGG 9 cut(s) 134, 157, 167, 353, 364, 365, 813, 879, 880
NdeII GATC 7 cut(s) 162, 243, 396, 447, 708, 819, 1041
NheI GCTAGC 1 cut(s) 764
NlaIII CATG 5 cut(s) 149, 523, 725, 839, 982
NmuCI GTSAC 6 cut(s) 347, 526, 613, 746, 851, 1061
PaeR7I CTCGAG 2 cut(s) 107, 206
PagI TCATGA 1 cut(s) 721
PcsI WCGNNNNNNNCGW 3 cut(s) 24, 33, 114
PfeI GAWTC 3 cut(s) 5, 938, 1174
PflMI CCANNNNNTGG 1 cut(s) 484
PfoI TCCNGGA 1 cut(s) 155
PkrI GCNGC 1 cut(s) 59
PleI GAGTC 3 cut(s) 160, 217, 244
PpsI GAGTC 3 cut(s) 160, 217, 244
Ppu21I YACGTR 1 cut(s) 174
PpuMI RGGWCCY 1 cut(s) 875
Psp5II RGGWCCY 1 cut(s) 875
Psp6I CCWGG 2 cut(s) 309, 855
PspEI GGTNACC 1 cut(s) 347
PspGI CCWGG 2 cut(s) 309, 855
PspOMI GGGCCC 1 cut(s) 947
PspPI GGNCC 7 cut(s) 88, 194, 286, 501, 875, 947, 948
PspPPI RGGWCCY 1 cut(s) 875
PspXI VCTCGAGB 2 cut(s) 107, 206
RsaI GTAC 6 cut(s) 204, 251, 386, 675, 793, 845
RsaNI GTAC 6 cut(s) 203, 250, 385, 674, 792, 844
RseI CAYNNNNRTG 1 cut(s) 524
SaqAI TTAA 3 cut(s) 537, 645, 1191
SatI GCNGC 1 cut(s) 58
Sau3AI GATC 7 cut(s) 162, 243, 396, 447, 708, 819, 1041
Sau96I GGNCC 7 cut(s) 88, 194, 286, 501, 875, 947, 948
ScaI AGTACT 1 cut(s) 386
SchI GAGTC 3 cut(s) 161, 218, 245
SduI GDGCHC 3 cut(s) 140, 438, 951
SfaNI GCATC 1 cut(s) 280
Sfr274I CTCGAG 2 cut(s) 107, 206
SinI GGWCC 2 cut(s) 194, 875
SlaI CTCGAG 2 cut(s) 107, 206
SmaI CCCGGG 2 cut(s) 365, 880
SmiMI CAYNNNNRTG 1 cut(s) 524
SmlI CTYRAG 4 cut(s) 107, 206, 1169, 1179
SmoI CTYRAG 4 cut(s) 107, 206, 1169, 1179
SnaBI TACGTA 1 cut(s) 174
Sse9I AATT 3 cut(s) 48, 113, 665
SsiI CCGC 6 cut(s) 66, 78, 830, 840, 887, 1050
SspMI CTAG 2 cut(s) 729, 765
StyI CCWWGG 2 cut(s) 459, 681
TaaI ACNGT 3 cut(s) 440, 619, 652
TaiI ACGT 2 cut(s) 30, 176
TaqI TCGA 6 cut(s) 24, 108, 117, 207, 331, 470
TasI AATT 3 cut(s) 48, 113, 665
TatI WGTACW 2 cut(s) 384, 673
TfiI GAWTC 3 cut(s) 5, 938, 1174
Tru1I TTAA 3 cut(s) 537, 645, 1191
Tru9I TTAA 3 cut(s) 537, 645, 1191
TscAI CASTG 1 cut(s) 697
TseFI GTSAC 6 cut(s) 347, 526, 613, 746, 851, 1061
TseI GCWGC 1 cut(s) 57
Tsp45I GTSAC 6 cut(s) 347, 526, 613, 746, 851, 1061
TspDTI ATGAA 3 cut(s) 561, 738, 1000
TspGWI ACGGA 2 cut(s) 446, 545
TspMI CCCGGG 2 cut(s) 363, 878
TspRI CASTG 1 cut(s) 697
Van91I CCANNNNNTGG 1 cut(s) 484
VpaK11BI GGWCC 2 cut(s) 194, 875
XhoI CTCGAG 2 cut(s) 107, 206
XmaI CCCGGG 2 cut(s) 363, 878
XspI CTAG 2 cut(s) 729, 765
ZraI GACGTC 1 cut(s) 28
ZrmI AGTACT 1 cut(s) 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.