RLG00000010916

Sarcosine oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0013564
Physical Location & Seq
Forward (+)
5379145 .. 5381416
2272 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010916

Sequence Viewer

Length: 1311 bp
ATGCGGCGTTGGGCACCCGAGGTGTGGGTGCCGCTGGGAGGGAAGCGAGGGGAGGAGGTCGGACTACCAACGCCGAGCCAGAGTGCCAACGCTCTGAAACCGGAGATGGAATCTTGCGTCCACGAGTTCGACGTCATAGTCGTCGGTGCCGGAATTATGGGCAGCTCCACCGCCTACCAGACCGCCAAGAGGGGCCACAAGACCCTCCTGCTCGAGCAGTTCGACTTCCTCCACCACCGGGGCTCGTCTCATGGCGAGTCCCGGACGATCCGGGCTACTTATCCAGAGGACTATTACGGTCCTCTGGTACTCGAGTCCTACAAGCTATGGCTACAAGCAGAGTCAGAGATCGGGTACAATGTCTATTTCAAGGCGCATCAGTTGGATATGGGCCAAGAAACCGACAGAGTTGTCCAGGCAGTTGTGGGTAGCTGTCGAAAGAACGAGCTTGGTCACCGGGTGCTGACCCGGGAGCAAGTGGAGCAGGAGTACTCGAGTCGGATCAAGATACCGGAGGGGTGGGTGGGTTTGGTTACGCAGCACGGTGGCGTGATCAAGCCCACCAAGGCGGTGTCGATGTTCCAAACTCTGGCCTTGCAAAACGGGGCCGTGCTGAGGGACAACATGGAGGTGACGGAGATTAAGAGAGATGAAGTGAGGGGAGGCATTTGGGTGGGGGTAGCAAATGGGGAGAGGTTTTGGGGTAAGAAATGTGTGGTCACAGTTGGGGCTTGGACTAAGAGGTTAGTTAAAACGGTTGGTGGGTTTGAATTGCCTATACAGCCCTTGGAGACCAGTGTGTGTTATTGGAGGATCAAGGAGGGTCATGAAGCTAGGTTTGCCATTGGAGGTGACTTCCCTACTTTTGCTAGCTATGGGGTTCCATATATCTATGGTACCCCCTCATTGGAGTATCCGGGTTTGATCAAGGTTGCGGTGCATGGCGGGTACCCGTGTGACCCGGATAAGAGGCCGTGGGGACCCGGGAACCCGCTGGCTCCGTTGAAGGAGTGGATAGAGCAGACATTCTCCGGCGTGGTTGATTCCGGAGGGCCGGTTGCCACTCAGTTGTGTATGTATTCCATGACCCCTGATGAGGATTATGTGATTGATTTCATTGGAGGTGAGTTCGGGAAGGATGTGGTGATCGGAGGCGGGTTTTCAGGTCACGGGTTCAAGCTGTCTCCGGTGGTGGGGAGGATATTGGCAGACCTTGCACTTAGTGGAGAGGCTAAAGGAGTGGAGCTAAAGCACTTTAGGATAGCAAGGTTTCAAGAGAATCCTCAAGGCAATGTTAAAGATTTCCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008115 GO:0008150 GO:0008152 GO:0016491 GO:0016645 GO:0016647 GO:0055114
KEGG Pathways
Metabolic & Signaling
ko00260 ko00310 ko01100 ko04146 map00260 map00310 map01100 map04146
Pfam Domains
Protein Families
DAO

Protein Analysis

437

Amino Acids

48.18

Weight (kDa)

6.34

Isoelectric Point (pI)

31.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 44 - 405 8.5e-46 FAD dependent oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013564)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24580
fragaria_vesca FvH4_2g36660
malus_domestica MD08G1126500.v1.1 MD15G1106400.v1.1
prunus_persica Prupe.1G458600_v2.0.a1 Prupe.1G458800_v2.0.a1 Prupe.1G458900_v2.0.a1
pyrus_communis pycom08g10570 pycom15g09710
rosa_chinensis RchiOBHm_Chr6g0305131
rosa_laevigata RLG00000010916
rosa_multiflora Rmu_sc0000061.1_g000020
rosa_rugosa Rorug06G0337900
rosa_samantha Rh6AG449500 Rh6BG451800 Rh6CG463000 Rh6DG450000
rosa_wichuraiana Rw6G039150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 137, 410
AatII GACGTC 1 cut(s) 135
Acc65I GGTACC 2 cut(s) 896, 948
AccB1I GGYRCC 5 cut(s) 13, 28, 146, 896, 948
AccB7I CCANNNNNTGG 1 cut(s) 589
AccIII TCCGGA 1 cut(s) 1046
AciI CCGC 9 cut(s) 4, 32, 171, 183, 569, 935, 945, 992, 1155
AclWI GGATC 3 cut(s) 262, 509, 821
AcyI GRCGYC 1 cut(s) 132
AdeI CACNNNGTG 2 cut(s) 460, 1223
AfaI GTAC 5 cut(s) 309, 356, 491, 898, 950
AfiI CCNNNNNNNGG 9 cut(s) 24, 38, 189, 238, 589, 615, 907, 1096, 1193
AgsI TTSAA 5 cut(s) 370, 770, 1006, 1177, 1274
AjnI CCWGG 1 cut(s) 414
AluBI AGCT 8 cut(s) 165, 325, 432, 448, 833, 873, 1180, 1246
AluI AGCT 8 cut(s) 165, 325, 432, 448, 833, 873, 1180, 1246
Alw26I GTCTC 3 cut(s) 252, 785, 1188
AlwI GGATC 3 cut(s) 262, 509, 821
Ama87I CYCGRG 6 cut(s) 17, 212, 311, 468, 493, 983
Aor13HI TCCGGA 1 cut(s) 1046
AoxI GGCC 6 cut(s) 193, 391, 591, 606, 971, 1052
ApeKI GCWGC 2 cut(s) 162, 538
Asp718I GGTACC 2 cut(s) 896, 948
AspLEI GCGC 1 cut(s) 376
AspS9I GGNCC 6 cut(s) 193, 299, 391, 606, 980, 1052
AsuHPI GGTGA 5 cut(s) 446, 643, 863, 1136, 1156
AsuNHI GCTAGC 1 cut(s) 869
AvaI CYCGRG 6 cut(s) 17, 212, 311, 468, 493, 983
AvaII GGWCC 2 cut(s) 299, 980
BaeGI GKGCMC 1 cut(s) 16
BaeI ACNNNNGTAYC 4 cut(s) 299, 332, 940, 973
BanI GGYRCC 5 cut(s) 13, 28, 146, 896, 948
BanII GRGCYC 1 cut(s) 245
BauI CACGAG 1 cut(s) 122
BbvCI CCTCAGC 1 cut(s) 614
BbvI GCAGC 2 cut(s) 174, 550
BccI CCATC 1 cut(s) 100
BceAI ACGGC 2 cut(s) 593, 958
BciT130I CCWGG 1 cut(s) 416
BciVI GTATCC 1 cut(s) 924
BclI TGATCA 2 cut(s) 552, 924
BcoDI GTCTC 3 cut(s) 252, 785, 1188
BfaI CTAG 2 cut(s) 834, 870
BfuI GTATCC 1 cut(s) 924
BisI GCNGC 4 cut(s) 5, 32, 163, 539
BlsI GCNGC 4 cut(s) 6, 33, 164, 540
BmcAI AGTACT 1 cut(s) 491
Bme18I GGWCC 2 cut(s) 299, 980
BmeT110I CYCGRG 6 cut(s) 17, 212, 311, 468, 493, 983
BmgT120I GGNCC 6 cut(s) 193, 299, 391, 606, 980, 1052
BmsI GCATC 1 cut(s) 385
BmtI GCTAGC 1 cut(s) 873
Bpu10I CCTNAGC 1 cut(s) 614
BpuEI CTTGAG 1 cut(s) 1269
BsaHI GRCGYC 1 cut(s) 132
BsaI GGTCTC 1 cut(s) 785
BsaJI CCNNGG 7 cut(s) 18, 238, 468, 564, 786, 974, 983
BsaWI WCCGGW 4 cut(s) 100, 511, 1046, 1186
BsaXI ACNNNNNCTCC 4 cut(s) 473, 503, 1041, 1071
Bsc4I CCNNNNNNNGG 9 cut(s) 24, 38, 189, 238, 589, 615, 907, 1096, 1193
Bse118I RCCGGY 1 cut(s) 1054
Bse1I ACTGG 1 cut(s) 795
Bse3DI GCAATG 1 cut(s) 1297
BseAI TCCGGA 1 cut(s) 1046
BseBI CCWGG 1 cut(s) 416
BseDI CCNNGG 7 cut(s) 18, 238, 468, 564, 786, 974, 983
BseGI GGATG 1 cut(s) 1144
BseLI CCNNNNNNNGG 9 cut(s) 24, 38, 189, 238, 589, 615, 907, 1096, 1193
BseMI GCAATG 1 cut(s) 1297
BseMII CTCAG 2 cut(s) 605, 1079
BseNI ACTGG 1 cut(s) 795
BseRI GAGGAG 1 cut(s) 68
BseSI GKGCMC 1 cut(s) 16
BseXI GCAGC 2 cut(s) 174, 550
BseYI CCCAGC 1 cut(s) 34
BshFI GGCC 6 cut(s) 195, 393, 593, 608, 973, 1054
BshNI GGYRCC 5 cut(s) 13, 28, 146, 896, 948
BsiHKCI CYCGRG 6 cut(s) 17, 212, 311, 468, 493, 983
BslFI GGGAC 3 cut(s) 244, 632, 993
BslI CCNNNNNNNGG 9 cut(s) 24, 38, 189, 238, 589, 615, 907, 1096, 1193
BsmAI GTCTC 3 cut(s) 252, 785, 1188
BsmBI CGTCTC 1 cut(s) 252
BsmFI GGGAC 3 cut(s) 244, 632, 993
BsnI GGCC 6 cut(s) 195, 393, 593, 608, 973, 1054
Bso31I GGTCTC 1 cut(s) 785
BsoBI CYCGRG 6 cut(s) 17, 212, 311, 468, 493, 983
Bsp1286I GDGCHC 2 cut(s) 16, 245
Bsp13I TCCGGA 1 cut(s) 1046
Bsp143I GATC 7 cut(s) 267, 348, 501, 552, 813, 924, 1146
BspACI CCGC 9 cut(s) 4, 32, 171, 183, 569, 935, 945, 992, 1155
BspANI GGCC 6 cut(s) 195, 393, 593, 608, 973, 1054
BspCNI CTCAG 2 cut(s) 606, 1078
BspEI TCCGGA 1 cut(s) 1046
BspHI TCATGA 1 cut(s) 826
BspOI GCTAGC 1 cut(s) 873
BspPI GGATC 3 cut(s) 262, 509, 821
BspT107I GGYRCC 5 cut(s) 13, 28, 146, 896, 948
BspTNI GGTCTC 1 cut(s) 785
BsrDI GCAATG 1 cut(s) 1297
BsrFI RCCGGY 1 cut(s) 1054
BsrI ACTGG 1 cut(s) 795
BssAI RCCGGY 1 cut(s) 1054
BssECI CCNNGG 7 cut(s) 18, 238, 468, 564, 786, 974, 983
BssMI GATC 7 cut(s) 267, 348, 501, 552, 813, 924, 1146
BssNI GRCGYC 1 cut(s) 132
BssSI CACGAG 1 cut(s) 122
BssT1I CCWWGG 2 cut(s) 564, 786
Bst2BI CACGAG 1 cut(s) 122
Bst2UI CCWGG 1 cut(s) 416
Bst4CI ACNGT 4 cut(s) 299, 545, 724, 757
BstACI GRCGYC 1 cut(s) 132
BstAPI GCANNNNNTGC 1 cut(s) 1214
BstC8I GCNNGC 2 cut(s) 871, 996
BstDEI CTNAG 4 cut(s) 614, 738, 1065, 1220
BstDSI CCRYGG 1 cut(s) 974
BstEII GGTNACC 1 cut(s) 452
BstF5I GGATG 1 cut(s) 1144
BstHHI GCGC 1 cut(s) 376
BstKTI GATC 7 cut(s) 270, 351, 504, 555, 816, 927, 1149
BstMAI GTCTC 3 cut(s) 252, 785, 1188
BstMBI GATC 7 cut(s) 267, 348, 501, 552, 813, 924, 1146
BstMWI GCNNNNNNNGC 4 cut(s) 481, 781, 839, 1214
BstNI CCWGG 1 cut(s) 416
BstPI GGTNACC 1 cut(s) 452
BstSLI GKGCMC 1 cut(s) 16
BstV1I GCAGC 2 cut(s) 174, 550
BsuI GTATCC 1 cut(s) 924
BsuRI GGCC 6 cut(s) 195, 393, 593, 608, 973, 1054
BtgI CCRYGG 1 cut(s) 974
BtsCI GGATG 1 cut(s) 1144
BtsIMutI CAGTG 1 cut(s) 802
Cac8I GCNNGC 2 cut(s) 871, 996
CciI TCATGA 1 cut(s) 826
CfoI GCGC 1 cut(s) 376
Cfr10I RCCGGY 1 cut(s) 1054
Cfr13I GGNCC 6 cut(s) 193, 299, 391, 606, 980, 1052
Cfr9I CCCGGG 2 cut(s) 468, 983
CseI GACGC 1 cut(s) 106
Csp6I GTAC 5 cut(s) 308, 355, 490, 897, 949
CviAII CATG 5 cut(s) 251, 625, 827, 941, 1084
CviQI GTAC 5 cut(s) 308, 355, 490, 897, 949
DdeI CTNAG 4 cut(s) 614, 738, 1065, 1220
DpnI GATC 7 cut(s) 269, 350, 503, 554, 815, 926, 1148
DpnII GATC 7 cut(s) 267, 348, 501, 552, 813, 924, 1146
DraIII CACNNNGTG 2 cut(s) 460, 1223
DrdI GACNNNNNNGTC 2 cut(s) 137, 410
DseDI GACNNNNNNGTC 2 cut(s) 137, 410
Eco130I CCWWGG 2 cut(s) 564, 786
Eco24I GRGCYC 1 cut(s) 245
Eco31I GGTCTC 1 cut(s) 785
Eco47I GGWCC 2 cut(s) 299, 980
Eco88I CYCGRG 6 cut(s) 17, 212, 311, 468, 493, 983
Eco91I GGTNACC 1 cut(s) 452
EcoO109I RGGNCCY 1 cut(s) 980
EcoO65I GGTNACC 1 cut(s) 452
EcoRII CCWGG 1 cut(s) 414
EcoT14I CCWWGG 2 cut(s) 564, 786
EcoT38I GRGCYC 1 cut(s) 245
ErhI CCWWGG 2 cut(s) 564, 786
Esp3I CGTCTC 1 cut(s) 252
FaeI CATG 5 cut(s) 254, 628, 830, 944, 1087
FaqI GGGAC 3 cut(s) 244, 632, 993
FatI CATG 5 cut(s) 250, 624, 826, 940, 1083
FauI CCCGC 3 cut(s) 938, 999, 1148
FbaI TGATCA 2 cut(s) 552, 924
Fnu4HI GCNGC 4 cut(s) 5, 32, 163, 539
FokI GGATG 1 cut(s) 1151
FriOI GRGCYC 1 cut(s) 245
Fsp4HI GCNGC 4 cut(s) 5, 32, 163, 539
FspBI CTAG 2 cut(s) 834, 870
GlaI GCGC 1 cut(s) 375
GluI GCNGC 4 cut(s) 5, 32, 163, 539
GsaI CCCAGC 1 cut(s) 38
HaeIII GGCC 6 cut(s) 195, 393, 593, 608, 973, 1054
HgaI GACGC 1 cut(s) 106
HhaI GCGC 1 cut(s) 376
Hin1I GRCGYC 1 cut(s) 132
Hin1II CATG 5 cut(s) 254, 628, 830, 944, 1087
Hin6I GCGC 1 cut(s) 374
HinP1I GCGC 1 cut(s) 374
HinfI GANTC 7 cut(s) 110, 257, 314, 341, 496, 1043, 1279
HphI GGTGA 5 cut(s) 446, 643, 863, 1136, 1156
Hpy166II GTNNAC 1 cut(s) 121
Hpy188I TCNGA 5 cut(s) 62, 96, 346, 501, 1151
Hpy188III TCNNGA 6 cut(s) 284, 505, 827, 1047, 1132, 1274
Hpy8I GTNNAC 1 cut(s) 121
Hpy99I CGWCG 2 cut(s) 134, 146
HpyAV CCTTC 2 cut(s) 1000, 1129
HpyCH4III ACNGT 4 cut(s) 299, 545, 724, 757
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 3 cut(s) 598, 940, 1217
HpyF10VI GCNNNNNNNGC 4 cut(s) 481, 781, 839, 1214
HpyF3I CTNAG 4 cut(s) 614, 738, 1065, 1220
HpySE526I ACGT 1 cut(s) 132
Hsp92I GRCGYC 1 cut(s) 132
Hsp92II CATG 5 cut(s) 254, 628, 830, 944, 1087
HspAI GCGC 1 cut(s) 374
KflI GGGWCCC 1 cut(s) 980
Kpn2I TCCGGA 1 cut(s) 1046
KpnI GGTACC 2 cut(s) 900, 952
Ksp22I TGATCA 2 cut(s) 552, 924
Kzo9I GATC 7 cut(s) 267, 348, 501, 552, 813, 924, 1146
LmnI GCTCC 5 cut(s) 170, 472, 481, 1003, 1243
Lsp1109I GCAGC 2 cut(s) 174, 550
LweI GCATC 1 cut(s) 385
MaeI CTAG 2 cut(s) 834, 870
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 7 cut(s) 452, 532, 631, 718, 851, 956, 1166
MalI GATC 7 cut(s) 269, 350, 503, 554, 815, 926, 1148
MboI GATC 7 cut(s) 267, 348, 501, 552, 813, 924, 1146
MhlI GDGCHC 2 cut(s) 16, 245
MluCI AATT 2 cut(s) 153, 770
MlyI GAGTC 4 cut(s) 266, 323, 350, 505
MmeI TCCRAC 3 cut(s) 40, 363, 479
MroI TCCGGA 1 cut(s) 1046
MseI TTAA 3 cut(s) 642, 750, 1296
MslI CAYNNNNRTG 2 cut(s) 629, 671
MspA1I CMGCKG 2 cut(s) 34, 994
MvaI CCWGG 1 cut(s) 416
MwoI GCNNNNNNNGC 4 cut(s) 481, 781, 839, 1214
NdeII GATC 7 cut(s) 267, 348, 501, 552, 813, 924, 1146
NheI GCTAGC 1 cut(s) 869
NlaIII CATG 5 cut(s) 254, 628, 830, 944, 1087
NmeAIII GCCGAG 1 cut(s) 99
NmuCI GTSAC 6 cut(s) 452, 631, 718, 851, 956, 1166
PaeR7I CTCGAG 3 cut(s) 212, 311, 493
PagI TCATGA 1 cut(s) 826
PcsI WCGNNNNNNNCGW 3 cut(s) 129, 138, 219
PfeI GAWTC 3 cut(s) 110, 1043, 1279
PflMI CCANNNNNTGG 1 cut(s) 589
PfoI TCCNGGA 1 cut(s) 260
PkrI GCNGC 4 cut(s) 6, 33, 164, 540
PleI GAGTC 4 cut(s) 265, 322, 349, 504
PpsI GAGTC 4 cut(s) 265, 322, 349, 504
PpuMI RGGWCCY 1 cut(s) 980
Psp5II RGGWCCY 1 cut(s) 980
Psp6I CCWGG 1 cut(s) 414
PspEI GGTNACC 1 cut(s) 452
PspFI CCCAGC 1 cut(s) 34
PspGI CCWGG 1 cut(s) 414
PspPI GGNCC 6 cut(s) 193, 299, 391, 606, 980, 1052
PspPPI RGGWCCY 1 cut(s) 980
PspXI VCTCGAGB 3 cut(s) 212, 311, 493
RsaI GTAC 5 cut(s) 309, 356, 491, 898, 950
RsaNI GTAC 5 cut(s) 308, 355, 490, 897, 949
RseI CAYNNNNRTG 2 cut(s) 629, 671
SaqAI TTAA 3 cut(s) 642, 750, 1296
SatI GCNGC 4 cut(s) 5, 32, 163, 539
Sau3AI GATC 7 cut(s) 267, 348, 501, 552, 813, 924, 1146
Sau96I GGNCC 6 cut(s) 193, 299, 391, 606, 980, 1052
ScaI AGTACT 1 cut(s) 491
SchI GAGTC 4 cut(s) 266, 323, 350, 505
SduI GDGCHC 2 cut(s) 16, 245
SfaNI GCATC 1 cut(s) 385
Sfr274I CTCGAG 3 cut(s) 212, 311, 493
SinI GGWCC 2 cut(s) 299, 980
SlaI CTCGAG 3 cut(s) 212, 311, 493
SmaI CCCGGG 2 cut(s) 470, 985
SmiMI CAYNNNNRTG 2 cut(s) 629, 671
SmlI CTYRAG 4 cut(s) 212, 311, 493, 1284
SmoI CTYRAG 4 cut(s) 212, 311, 493, 1284
Sse9I AATT 2 cut(s) 153, 770
SsiI CCGC 9 cut(s) 4, 32, 171, 183, 569, 935, 945, 992, 1155
SspMI CTAG 2 cut(s) 834, 870
StyI CCWWGG 2 cut(s) 564, 786
TaaI ACNGT 4 cut(s) 299, 545, 724, 757
TaiI ACGT 1 cut(s) 135
TaqI TCGA 7 cut(s) 129, 213, 222, 312, 436, 494, 575
TasI AATT 2 cut(s) 153, 770
TatI WGTACW 1 cut(s) 489
TauI GCSGC 2 cut(s) 7, 34
TfiI GAWTC 3 cut(s) 110, 1043, 1279
Tru1I TTAA 3 cut(s) 642, 750, 1296
Tru9I TTAA 3 cut(s) 642, 750, 1296
TscAI CASTG 1 cut(s) 802
TseFI GTSAC 6 cut(s) 452, 631, 718, 851, 956, 1166
TseI GCWGC 2 cut(s) 162, 538
Tsp45I GTSAC 6 cut(s) 452, 631, 718, 851, 956, 1166
TspDTI ATGAA 3 cut(s) 666, 843, 1105
TspGWI ACGGA 2 cut(s) 650, 990
TspMI CCCGGG 2 cut(s) 468, 983
TspRI CASTG 1 cut(s) 802
Van91I CCANNNNNTGG 1 cut(s) 589
VpaK11BI GGWCC 2 cut(s) 299, 980
XhoI CTCGAG 3 cut(s) 212, 311, 493
XmaI CCCGGG 2 cut(s) 468, 983
XspI CTAG 2 cut(s) 834, 870
ZraI GACGTC 1 cut(s) 133
ZrmI AGTACT 1 cut(s) 491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.