Rh6CG463000

Sarcosine oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0013564
Physical Location & Seq
Reverse (-)
63611539 .. 63612891
1353 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG463000.1

Sequence Viewer

Length: 1233 bp
ATGTCCTCTTCCACGCAGAAACCGGAGATGGAATCTTCCGTCCACGAGTTCGACGTCATAGTCGTCGGTGCCGGAATTATGGGCAGCTCCACCGCCTACCAGACCGCCAAGAGGGGCCACAAGACCCTCCTGCTCGAGCAATTCGACTTCCTCCACCACCGGGGCTCGTCTCATGGCGAGTCCCGGACGATCCGGGCTACGTATCCAGATGACTATTACGGTCCTCTGGTACTCGAGTCCTACAAGCTATGGCTACAAGCAGAGTCAGAGATCGGGTACAATGTCTATTTCAAGGCGCATCAGTTGGATATGGGCCAAGAAACCGACAGAGTTGTCCAGGCAGTTGTGGGTAGCTGTCGAAAGAACGAGCTTGGTCACCGGGTGCTGACCCGGGAGCAAGTGGAGCAGGAGTACTCGGGTCGGATCAAGATACCGGAGGGGTGGGTGGGTTTGGTTACGGAGCACGGTGGCGTGATCAAGCCCACCAAGGCGGTGTCGATGTTCCAAACTCTGGCCTTGCAAAACGGCGCCGTGCTGAGGGACAACATGGAGGTGACGGAGATTAAGAGAGATGAAGTGAGGGGAGGCATTTGGGTGGGGGTAGCAAATGGGGAGAGGTTTTGGGGTAAGAAATGTGTTGTCACAGTTGGGGCTTGGACTAAGAGGTTAGTTAAAACGGTTGGTGGGTTTGAATTGCCTATACAGCCCTTGGAGACCAATGTGTGTTATTGGAGGATCAAGGAGGGTCATGAAGCTAGGTTTGCCATTGGAGGTGACTTCCCTACTTTTGCTAGCTATGGGGTTCCATATATCTATGGTACCCCCTCATTGGAGTATCCGGGTTTGATTAAGGTTGCGGTGCATGGCGGGTACCCGTGTGACCCGGATAAGAGGCCGTGGGGACCCGGGAACCCGCTGGCTCCGTTGAAGGAGTGGATAGAGCAGACATTCTCCGGCATGGTTGATTCCGGAGGGCCGGTTGCCACTCAGTTGTGTATGTATTCCATGACCCCAGATGAGGATTATGTGATTGATTTCATTGGAGGTGAGTTCGGGAAGGATGTGGTGATCGGAGGCGGGTTTTCAGGTCACGGGTTCAAGCTGTCTCCGGTGGTGGGGAGGATATTGGCAGACCTTGCACTTAGTGGAGAGGCTAAAGGAGTGGAGCTAAAGCACTTTAGGATAGCAAGGTTTCAAGAGAATCCTCAAGGCAACGTTAAAGATTTCCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008115 GO:0008150 GO:0008152 GO:0016491 GO:0016645 GO:0016647 GO:0055114
KEGG Pathways
Metabolic & Signaling
ko00260 ko00310 ko01100 ko04146 map00260 map00310 map01100 map04146
Pfam Domains
Protein Families
DAO

Protein Analysis

410

Amino Acids

45.33

Weight (kDa)

6.09

Isoelectric Point (pI)

26.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 18 - 379 8.6e-46 FAD dependent oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013564)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24580
fragaria_vesca FvH4_2g36660
malus_domestica MD08G1126500.v1.1 MD15G1106400.v1.1
prunus_persica Prupe.1G458600_v2.0.a1 Prupe.1G458800_v2.0.a1 Prupe.1G458900_v2.0.a1
pyrus_communis pycom08g10570 pycom15g09710
rosa_chinensis RchiOBHm_Chr6g0305131
rosa_laevigata RLG00000010916
rosa_multiflora Rmu_sc0000061.1_g000020
rosa_rugosa Rorug06G0337900
rosa_samantha Rh6AG449500 Rh6BG451800 Rh6CG463000 Rh6DG450000
rosa_wichuraiana Rw6G039150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 59, 332
AatII GACGTC 1 cut(s) 57
Acc65I GGTACC 2 cut(s) 818, 870
AccB1I GGYRCC 4 cut(s) 68, 527, 818, 870
AccB7I CCANNNNNTGG 1 cut(s) 511
AccIII TCCGGA 1 cut(s) 968
AciI CCGC 7 cut(s) 93, 105, 491, 857, 867, 914, 1077
AclI AACGTT 1 cut(s) 1215
AclWI GGATC 3 cut(s) 184, 431, 743
AcyI GRCGYC 2 cut(s) 54, 528
AdeI CACNNNGTG 2 cut(s) 382, 1145
AfaI GTAC 5 cut(s) 231, 278, 413, 820, 872
AfiI CCNNNNNNNGG 7 cut(s) 111, 160, 511, 537, 829, 1018, 1115
AgsI TTSAA 5 cut(s) 292, 692, 928, 1099, 1196
AjnI CCWGG 1 cut(s) 336
AluBI AGCT 8 cut(s) 87, 247, 354, 370, 755, 795, 1102, 1168
AluI AGCT 8 cut(s) 87, 247, 354, 370, 755, 795, 1102, 1168
Alw21I GWGCWC 1 cut(s) 465
Alw26I GTCTC 3 cut(s) 174, 707, 1110
AlwI GGATC 3 cut(s) 184, 431, 743
Ama87I CYCGRG 5 cut(s) 134, 233, 390, 415, 905
Aor13HI TCCGGA 1 cut(s) 968
AoxI GGCC 5 cut(s) 115, 313, 513, 893, 974
ApeKI GCWGC 1 cut(s) 84
Asp718I GGTACC 2 cut(s) 818, 870
AspLEI GCGC 2 cut(s) 298, 530
AspS9I GGNCC 5 cut(s) 115, 221, 313, 902, 974
AsuHPI GGTGA 5 cut(s) 368, 565, 785, 1058, 1078
AsuNHI GCTAGC 1 cut(s) 791
AvaI CYCGRG 5 cut(s) 134, 233, 390, 415, 905
AvaII GGWCC 2 cut(s) 221, 902
BaeI ACNNNNGTAYC 4 cut(s) 221, 254, 862, 895
BanI GGYRCC 4 cut(s) 68, 527, 818, 870
BanII GRGCYC 1 cut(s) 167
BauI CACGAG 1 cut(s) 44
Bbv12I GWGCWC 1 cut(s) 465
BbvCI CCTCAGC 1 cut(s) 536
BbvI GCAGC 1 cut(s) 96
BccI CCATC 1 cut(s) 22
BceAI ACGGC 3 cut(s) 515, 541, 880
BciT130I CCWGG 1 cut(s) 338
BciVI GTATCC 2 cut(s) 213, 846
BclI TGATCA 1 cut(s) 474
BcoDI GTCTC 3 cut(s) 174, 707, 1110
BfaI CTAG 2 cut(s) 756, 792
BfoI RGCGCY 1 cut(s) 531
BfuI GTATCC 2 cut(s) 213, 846
BisI GCNGC 1 cut(s) 85
BlsI GCNGC 1 cut(s) 86
BmcAI AGTACT 1 cut(s) 413
Bme18I GGWCC 2 cut(s) 221, 902
BmeT110I CYCGRG 5 cut(s) 134, 233, 390, 415, 905
BmgT120I GGNCC 5 cut(s) 115, 221, 313, 902, 974
BmsI GCATC 1 cut(s) 307
BmtI GCTAGC 1 cut(s) 795
Bpu10I CCTNAGC 1 cut(s) 536
BpuEI CTTGAG 1 cut(s) 1191
BsaAI YACGTR 1 cut(s) 201
BsaHI GRCGYC 2 cut(s) 54, 528
BsaI GGTCTC 1 cut(s) 707
BsaJI CCNNGG 6 cut(s) 160, 390, 486, 708, 896, 905
BsaWI WCCGGW 4 cut(s) 22, 433, 968, 1108
BsaXI ACNNNNNCTCC 4 cut(s) 395, 425, 963, 993
Bsc4I CCNNNNNNNGG 7 cut(s) 111, 160, 511, 537, 829, 1018, 1115
Bse118I RCCGGY 1 cut(s) 976
BseAI TCCGGA 1 cut(s) 968
BseBI CCWGG 1 cut(s) 338
BseDI CCNNGG 6 cut(s) 160, 390, 486, 708, 896, 905
BseGI GGATG 1 cut(s) 1066
BseLI CCNNNNNNNGG 7 cut(s) 111, 160, 511, 537, 829, 1018, 1115
BseMII CTCAG 2 cut(s) 527, 1001
BseXI GCAGC 1 cut(s) 96
BshFI GGCC 5 cut(s) 117, 315, 515, 895, 976
BshNI GGYRCC 4 cut(s) 68, 527, 818, 870
BsiHKAI GWGCWC 1 cut(s) 465
BsiHKCI CYCGRG 5 cut(s) 134, 233, 390, 415, 905
BslFI GGGAC 3 cut(s) 166, 554, 915
BslI CCNNNNNNNGG 7 cut(s) 111, 160, 511, 537, 829, 1018, 1115
BsmAI GTCTC 3 cut(s) 174, 707, 1110
BsmBI CGTCTC 1 cut(s) 174
BsmFI GGGAC 3 cut(s) 166, 554, 915
BsnI GGCC 5 cut(s) 117, 315, 515, 895, 976
Bso31I GGTCTC 1 cut(s) 707
BsoBI CYCGRG 5 cut(s) 134, 233, 390, 415, 905
Bsp1286I GDGCHC 2 cut(s) 167, 465
Bsp13I TCCGGA 1 cut(s) 968
Bsp143I GATC 6 cut(s) 189, 270, 423, 474, 735, 1068
BspACI CCGC 7 cut(s) 93, 105, 491, 857, 867, 914, 1077
BspANI GGCC 5 cut(s) 117, 315, 515, 895, 976
BspCNI CTCAG 2 cut(s) 528, 1000
BspEI TCCGGA 1 cut(s) 968
BspHI TCATGA 1 cut(s) 748
BspOI GCTAGC 1 cut(s) 795
BspPI GGATC 3 cut(s) 184, 431, 743
BspT107I GGYRCC 4 cut(s) 68, 527, 818, 870
BspTNI GGTCTC 1 cut(s) 707
BsrFI RCCGGY 1 cut(s) 976
BssAI RCCGGY 1 cut(s) 976
BssECI CCNNGG 6 cut(s) 160, 390, 486, 708, 896, 905
BssMI GATC 6 cut(s) 189, 270, 423, 474, 735, 1068
BssNI GRCGYC 2 cut(s) 54, 528
BssSI CACGAG 1 cut(s) 44
BssT1I CCWWGG 2 cut(s) 486, 708
Bst2BI CACGAG 1 cut(s) 44
Bst2UI CCWGG 1 cut(s) 338
Bst4CI ACNGT 4 cut(s) 221, 467, 646, 679
Bst6I CTCTTC 1 cut(s) 13
BstACI GRCGYC 2 cut(s) 54, 528
BstAPI GCANNNNNTGC 1 cut(s) 1136
BstBAI YACGTR 1 cut(s) 201
BstC8I GCNNGC 2 cut(s) 793, 918
BstDEI CTNAG 4 cut(s) 536, 660, 987, 1142
BstDSI CCRYGG 1 cut(s) 896
BstEII GGTNACC 1 cut(s) 374
BstF5I GGATG 1 cut(s) 1066
BstH2I RGCGCY 1 cut(s) 531
BstHHI GCGC 2 cut(s) 298, 530
BstKTI GATC 6 cut(s) 192, 273, 426, 477, 738, 1071
BstMAI GTCTC 3 cut(s) 174, 707, 1110
BstMBI GATC 6 cut(s) 189, 270, 423, 474, 735, 1068
BstMWI GCNNNNNNNGC 4 cut(s) 403, 703, 761, 1136
BstNI CCWGG 1 cut(s) 338
BstPI GGTNACC 1 cut(s) 374
BstSNI TACGTA 1 cut(s) 201
BstV1I GCAGC 1 cut(s) 96
BsuI GTATCC 2 cut(s) 213, 846
BsuRI GGCC 5 cut(s) 117, 315, 515, 895, 976
BtgI CCRYGG 1 cut(s) 896
BtsCI GGATG 1 cut(s) 1066
Cac8I GCNNGC 2 cut(s) 793, 918
CciI TCATGA 1 cut(s) 748
CfoI GCGC 2 cut(s) 298, 530
Cfr10I RCCGGY 1 cut(s) 976
Cfr13I GGNCC 5 cut(s) 115, 221, 313, 902, 974
Cfr9I CCCGGG 2 cut(s) 390, 905
Csp6I GTAC 5 cut(s) 230, 277, 412, 819, 871
CviAII CATG 6 cut(s) 173, 547, 749, 863, 958, 1006
CviQI GTAC 5 cut(s) 230, 277, 412, 819, 871
DdeI CTNAG 4 cut(s) 536, 660, 987, 1142
DinI GGCGCC 1 cut(s) 529
DpnI GATC 6 cut(s) 191, 272, 425, 476, 737, 1070
DpnII GATC 6 cut(s) 189, 270, 423, 474, 735, 1068
DraIII CACNNNGTG 2 cut(s) 382, 1145
DrdI GACNNNNNNGTC 2 cut(s) 59, 332
DseDI GACNNNNNNGTC 2 cut(s) 59, 332
Eam1104I CTCTTC 1 cut(s) 13
EarI CTCTTC 1 cut(s) 13
Eco105I TACGTA 1 cut(s) 201
Eco130I CCWWGG 2 cut(s) 486, 708
Eco24I GRGCYC 1 cut(s) 167
Eco31I GGTCTC 1 cut(s) 707
Eco47I GGWCC 2 cut(s) 221, 902
Eco88I CYCGRG 5 cut(s) 134, 233, 390, 415, 905
Eco91I GGTNACC 1 cut(s) 374
EcoO109I RGGNCCY 1 cut(s) 902
EcoO65I GGTNACC 1 cut(s) 374
EcoRII CCWGG 1 cut(s) 336
EcoT14I CCWWGG 2 cut(s) 486, 708
EcoT38I GRGCYC 1 cut(s) 167
EgeI GGCGCC 1 cut(s) 529
EheI GGCGCC 1 cut(s) 529
ErhI CCWWGG 2 cut(s) 486, 708
Esp3I CGTCTC 1 cut(s) 174
FaeI CATG 6 cut(s) 176, 550, 752, 866, 961, 1009
FaqI GGGAC 3 cut(s) 166, 554, 915
FatI CATG 6 cut(s) 172, 546, 748, 862, 957, 1005
FauI CCCGC 3 cut(s) 860, 921, 1070
FbaI TGATCA 1 cut(s) 474
Fnu4HI GCNGC 1 cut(s) 85
FokI GGATG 1 cut(s) 1073
FriOI GRGCYC 1 cut(s) 167
Fsp4HI GCNGC 1 cut(s) 85
FspBI CTAG 2 cut(s) 756, 792
GlaI GCGC 2 cut(s) 297, 529
GluI GCNGC 1 cut(s) 85
HaeII RGCGCY 1 cut(s) 531
HaeIII GGCC 5 cut(s) 117, 315, 515, 895, 976
HhaI GCGC 2 cut(s) 298, 530
Hin1I GRCGYC 2 cut(s) 54, 528
Hin1II CATG 6 cut(s) 176, 550, 752, 866, 961, 1009
Hin6I GCGC 2 cut(s) 296, 528
HinP1I GCGC 2 cut(s) 296, 528
HinfI GANTC 6 cut(s) 32, 179, 236, 263, 965, 1201
HphI GGTGA 5 cut(s) 368, 565, 785, 1058, 1078
Hpy166II GTNNAC 1 cut(s) 43
Hpy188I TCNGA 3 cut(s) 268, 423, 1073
Hpy188III TCNNGA 6 cut(s) 206, 427, 749, 969, 1054, 1196
Hpy8I GTNNAC 1 cut(s) 43
Hpy99I CGWCG 2 cut(s) 56, 68
HpyAV CCTTC 2 cut(s) 922, 1051
HpyCH4III ACNGT 4 cut(s) 221, 467, 646, 679
HpyCH4IV ACGT 3 cut(s) 54, 200, 1215
HpyCH4V TGCA 3 cut(s) 520, 862, 1139
HpyF10VI GCNNNNNNNGC 4 cut(s) 403, 703, 761, 1136
HpyF3I CTNAG 4 cut(s) 536, 660, 987, 1142
HpySE526I ACGT 3 cut(s) 54, 200, 1215
Hsp92I GRCGYC 2 cut(s) 54, 528
Hsp92II CATG 6 cut(s) 176, 550, 752, 866, 961, 1009
HspAI GCGC 2 cut(s) 296, 528
KasI GGCGCC 1 cut(s) 527
KflI GGGWCCC 1 cut(s) 902
Kpn2I TCCGGA 1 cut(s) 968
KpnI GGTACC 2 cut(s) 822, 874
Ksp22I TGATCA 1 cut(s) 474
Kzo9I GATC 6 cut(s) 189, 270, 423, 474, 735, 1068
LmnI GCTCC 6 cut(s) 92, 394, 403, 460, 925, 1165
Lsp1109I GCAGC 1 cut(s) 96
LweI GCATC 1 cut(s) 307
MaeI CTAG 2 cut(s) 756, 792
MaeII ACGT 3 cut(s) 54, 200, 1215
MaeIII GTNAC 7 cut(s) 374, 454, 553, 640, 773, 878, 1088
MalI GATC 6 cut(s) 191, 272, 425, 476, 737, 1070
MboI GATC 6 cut(s) 189, 270, 423, 474, 735, 1068
MboII GAAGA 1 cut(s) 27
MhlI GDGCHC 2 cut(s) 167, 465
MluCI AATT 3 cut(s) 75, 140, 692
Mly113I GGCGCC 1 cut(s) 528
MlyI GAGTC 3 cut(s) 188, 245, 272
MmeI TCCRAC 2 cut(s) 285, 401
MroI TCCGGA 1 cut(s) 968
MseI TTAA 4 cut(s) 564, 672, 849, 1218
MslI CAYNNNNRTG 2 cut(s) 551, 593
MspA1I CMGCKG 1 cut(s) 916
MvaI CCWGG 1 cut(s) 338
MwoI GCNNNNNNNGC 4 cut(s) 403, 703, 761, 1136
NarI GGCGCC 1 cut(s) 528
NdeII GATC 6 cut(s) 189, 270, 423, 474, 735, 1068
NheI GCTAGC 1 cut(s) 791
NlaIII CATG 6 cut(s) 176, 550, 752, 866, 961, 1009
NmuCI GTSAC 6 cut(s) 374, 553, 640, 773, 878, 1088
PaeR7I CTCGAG 2 cut(s) 134, 233
PagI TCATGA 1 cut(s) 748
PcsI WCGNNNNNNNCGW 3 cut(s) 51, 60, 141
PfeI GAWTC 3 cut(s) 32, 965, 1201
PflMI CCANNNNNTGG 1 cut(s) 511
PfoI TCCNGGA 1 cut(s) 182
PkrI GCNGC 1 cut(s) 86
PleI GAGTC 3 cut(s) 187, 244, 271
PluTI GGCGCC 1 cut(s) 531
PpsI GAGTC 3 cut(s) 187, 244, 271
Ppu21I YACGTR 1 cut(s) 201
PpuMI RGGWCCY 1 cut(s) 902
Psp1406I AACGTT 1 cut(s) 1215
Psp5II RGGWCCY 1 cut(s) 902
Psp6I CCWGG 1 cut(s) 336
PspEI GGTNACC 1 cut(s) 374
PspGI CCWGG 1 cut(s) 336
PspPI GGNCC 5 cut(s) 115, 221, 313, 902, 974
PspPPI RGGWCCY 1 cut(s) 902
PspXI VCTCGAGB 2 cut(s) 134, 233
RsaI GTAC 5 cut(s) 231, 278, 413, 820, 872
RsaNI GTAC 5 cut(s) 230, 277, 412, 819, 871
RseI CAYNNNNRTG 2 cut(s) 551, 593
SaqAI TTAA 4 cut(s) 564, 672, 849, 1218
SatI GCNGC 1 cut(s) 85
Sau3AI GATC 6 cut(s) 189, 270, 423, 474, 735, 1068
Sau96I GGNCC 5 cut(s) 115, 221, 313, 902, 974
ScaI AGTACT 1 cut(s) 413
SchI GAGTC 3 cut(s) 188, 245, 272
SduI GDGCHC 2 cut(s) 167, 465
SfaNI GCATC 1 cut(s) 307
SfoI GGCGCC 1 cut(s) 529
Sfr274I CTCGAG 2 cut(s) 134, 233
SinI GGWCC 2 cut(s) 221, 902
SlaI CTCGAG 2 cut(s) 134, 233
SmaI CCCGGG 2 cut(s) 392, 907
SmiMI CAYNNNNRTG 2 cut(s) 551, 593
SmlI CTYRAG 3 cut(s) 134, 233, 1206
SmoI CTYRAG 3 cut(s) 134, 233, 1206
SnaBI TACGTA 1 cut(s) 201
Sse9I AATT 3 cut(s) 75, 140, 692
SsiI CCGC 7 cut(s) 93, 105, 491, 857, 867, 914, 1077
SspDI GGCGCC 1 cut(s) 527
SspMI CTAG 2 cut(s) 756, 792
StyI CCWWGG 2 cut(s) 486, 708
TaaI ACNGT 4 cut(s) 221, 467, 646, 679
TaiI ACGT 3 cut(s) 57, 203, 1218
TaqI TCGA 6 cut(s) 51, 135, 144, 234, 358, 497
TasI AATT 3 cut(s) 75, 140, 692
TatI WGTACW 1 cut(s) 411
TfiI GAWTC 3 cut(s) 32, 965, 1201
Tru1I TTAA 4 cut(s) 564, 672, 849, 1218
Tru9I TTAA 4 cut(s) 564, 672, 849, 1218
TseFI GTSAC 6 cut(s) 374, 553, 640, 773, 878, 1088
TseI GCWGC 1 cut(s) 84
Tsp45I GTSAC 6 cut(s) 374, 553, 640, 773, 878, 1088
TspDTI ATGAA 3 cut(s) 588, 765, 1027
TspGWI ACGGA 4 cut(s) 28, 473, 572, 912
TspMI CCCGGG 2 cut(s) 390, 905
Van91I CCANNNNNTGG 1 cut(s) 511
VpaK11BI GGWCC 2 cut(s) 221, 902
XhoI CTCGAG 2 cut(s) 134, 233
XmaI CCCGGG 2 cut(s) 390, 905
XspI CTAG 2 cut(s) 756, 792
ZraI GACGTC 1 cut(s) 55
ZrmI AGTACT 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.