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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT3G18310 |
| fragaria_vesca | FvH4_5g25850 |
| malus_domestica | MD04G1026400.v1.1 |
| prunus_persica | Prupe.2G050600_v2.0.a1 |
| pyrus_communis | pycom04g02250 |
| rosa_chinensis | RchiOBHm_Chr6g0264721 RchiOBHm_Chr6g0264731 RchiOBHm_Chr7g0216331 |
| rosa_laevigata | RLG00000002581 |
| rosa_multiflora | Rmu_sc0015060.1_g000019 |
| rosa_roxburghii | Rroxscaffold_3G00243320 |
| rosa_rugosa | Rorug07G0160000 |
| rosa_samantha | Rh7AG297400 Rh7BG289700 Rh7CG317600 Rh7DG299800 |
| rosa_wichuraiana | Rw7G025300 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 2520 |
| AatII | GACGTC | 1 cut(s) | 2411 |
| Acc16I | TGCGCA | 1 cut(s) | 1145 |
| Acc36I | ACCTGC | 2 cut(s) | 1073, 2338 |
| AccB1I | GGYRCC | 1 cut(s) | 930 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 492 |
| AciI | CCGC | 7 cut(s) | 290, 356, 490, 1449, 1980, 2170, 2486 |
| AclWI | GGATC | 2 cut(s) | 1363, 2503 |
| AcuI | CTGAAG | 2 cut(s) | 1398, 2678 |
| AcyI | GRCGYC | 1 cut(s) | 2408 |
| AfaI | GTAC | 6 cut(s) | 605, 714, 1668, 1724, 1769, 2121 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 107, 492, 535, 536 |
| AgeI | ACCGGT | 2 cut(s) | 389, 2354 |
| AjnI | CCWGG | 4 cut(s) | 105, 485, 863, 2200 |
| Alw21I | GWGCWC | 1 cut(s) | 1410 |
| Alw26I | GTCTC | 4 cut(s) | 15, 324, 2421, 2426 |
| AlwI | GGATC | 2 cut(s) | 1363, 2503 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1088 |
| Ama87I | CYCGRG | 1 cut(s) | 1270 |
| AoxI | GGCC | 4 cut(s) | 286, 987, 1180, 2046 |
| ApaI | GGGCCC | 2 cut(s) | 290, 991 |
| ApeKI | GCWGC | 3 cut(s) | 1510, 2172, 2328 |
| AsiGI | ACCGGT | 2 cut(s) | 389, 2354 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1391 |
| AspA2I | CCTAGG | 1 cut(s) | 1754 |
| AspLEI | GCGC | 1 cut(s) | 1146 |
| AspS9I | GGNCC | 9 cut(s) | 110, 286, 287, 644, 846, 987, 988, 1331, 2219 |
| AsuC2I | CCSGG | 1 cut(s) | 537 |
| AsuHPI | GGTGA | 5 cut(s) | 404, 782, 2216, 2344, 2369 |
| AsuII | TTCGAA | 2 cut(s) | 1076, 1367 |
| AvaI | CYCGRG | 1 cut(s) | 1270 |
| AvaII | GGWCC | 5 cut(s) | 110, 644, 846, 1331, 2219 |
| AvrII | CCTAGG | 1 cut(s) | 1754 |
| AxyI | CCTNAGG | 1 cut(s) | 1621 |
| BaeGI | GKGCMC | 4 cut(s) | 290, 356, 864, 991 |
| BanI | GGYRCC | 1 cut(s) | 930 |
| BanII | GRGCYC | 4 cut(s) | 290, 991, 1177, 1410 |
| BauI | CACGAG | 1 cut(s) | 1991 |
| BbsI | GAAGAC | 2 cut(s) | 52, 2154 |
| Bbv12I | GWGCWC | 1 cut(s) | 1410 |
| BbvI | GCAGC | 3 cut(s) | 1522, 2184, 2340 |
| BccI | CCATC | 3 cut(s) | 110, 872, 2417 |
| BciT130I | CCWGG | 4 cut(s) | 107, 487, 865, 2202 |
| BciVI | GTATCC | 2 cut(s) | 322, 2584 |
| BclI | TGATCA | 2 cut(s) | 403, 1852 |
| BcnI | CCSGG | 1 cut(s) | 537 |
| BcoDI | GTCTC | 4 cut(s) | 15, 324, 2421, 2426 |
| BfaI | CTAG | 4 cut(s) | 936, 1730, 1755, 2603 |
| BfmI | CTRYAG | 4 cut(s) | 347, 1944, 2115, 2628 |
| BfuAI | ACCTGC | 2 cut(s) | 1073, 2338 |
| BfuI | GTATCC | 2 cut(s) | 322, 2584 |
| BglII | AGATCT | 3 cut(s) | 15, 1417, 1555 |
| BisI | GCNGC | 5 cut(s) | 1511, 2170, 2173, 2329, 2487 |
| BlnI | CCTAGG | 1 cut(s) | 1754 |
| BlsI | GCNGC | 5 cut(s) | 1512, 2171, 2174, 2330, 2488 |
| Bme1390I | CCNGG | 5 cut(s) | 107, 487, 537, 865, 2202 |
| Bme18I | GGWCC | 5 cut(s) | 110, 644, 846, 1331, 2219 |
| BmeT110I | CYCGRG | 1 cut(s) | 1270 |
| BmgT120I | GGNCC | 9 cut(s) | 110, 286, 287, 644, 846, 987, 988, 1331, 2219 |
| BmiI | GGNNCC | 6 cut(s) | 111, 288, 847, 932, 989, 2220 |
| BmrFI | CCNGG | 5 cut(s) | 107, 487, 537, 865, 2202 |
| BmsI | GCATC | 6 cut(s) | 952, 1146, 1661, 1900, 2197, 2635 |
| BoxI | GACNNNNGTC | 1 cut(s) | 798 |
| BpiI | GAAGAC | 2 cut(s) | 52, 2154 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 2431, 2463 |
| Bpu14I | TTCGAA | 2 cut(s) | 1076, 1367 |
| BpuMI | CCSGG | 1 cut(s) | 537 |
| BsaHI | GRCGYC | 1 cut(s) | 2408 |
| BsaJI | CCNNGG | 5 cut(s) | 106, 536, 863, 864, 1754 |
| BsaWI | WCCGGW | 2 cut(s) | 389, 2354 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1323, 1353 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 107, 492, 535, 536 |
| Bse118I | RCCGGY | 2 cut(s) | 389, 2354 |
| Bse1I | ACTGG | 5 cut(s) | 1160, 2049, 2222, 2402, 2480 |
| Bse21I | CCTNAGG | 1 cut(s) | 1621 |
| Bse3DI | GCAATG | 3 cut(s) | 1175, 1444, 2272 |
| BseBI | CCWGG | 4 cut(s) | 107, 487, 865, 2202 |
| BseDI | CCNNGG | 5 cut(s) | 106, 536, 863, 864, 1754 |
| BseGI | GGATG | 5 cut(s) | 102, 466, 1249, 2276, 2530 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 107, 492, 535, 536 |
| BseMI | GCAATG | 3 cut(s) | 1175, 1444, 2272 |
| BseMII | CTCAG | 7 cut(s) | 26, 69, 1635, 1890, 2147, 2370, 2376 |
| BseNI | ACTGG | 5 cut(s) | 1160, 2049, 2222, 2402, 2480 |
| BseRI | GAGGAG | 4 cut(s) | 65, 212, 247, 314 |
| BseSI | GKGCMC | 4 cut(s) | 290, 356, 864, 991 |
| BseXI | GCAGC | 3 cut(s) | 1522, 2184, 2340 |
| BseYI | CCCAGC | 1 cut(s) | 1342 |
| BshFI | GGCC | 4 cut(s) | 288, 989, 1182, 2048 |
| BshNI | GGYRCC | 1 cut(s) | 930 |
| BshTI | ACCGGT | 2 cut(s) | 389, 2354 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1410 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1270 |
| BsiSI | CCGG | 3 cut(s) | 390, 536, 2355 |
| BslFI | GGGAC | 2 cut(s) | 859, 2395 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 107, 492, 535, 536 |
| BsmAI | GTCTC | 4 cut(s) | 15, 324, 2421, 2426 |
| BsmBI | CGTCTC | 3 cut(s) | 15, 324, 2421 |
| BsmFI | GGGAC | 2 cut(s) | 859, 2395 |
| BsmI | GAATGC | 1 cut(s) | 829 |
| BsnI | GGCC | 4 cut(s) | 288, 989, 1182, 2048 |
| BsoBI | CYCGRG | 1 cut(s) | 1270 |
| Bsp119I | TTCGAA | 2 cut(s) | 1076, 1367 |
| Bsp120I | GGGCCC | 2 cut(s) | 286, 987 |
| Bsp1286I | GDGCHC | 6 cut(s) | 290, 356, 864, 991, 1177, 1410 |
| Bsp1407I | TGTACA | 2 cut(s) | 712, 1666 |
| BspACI | CCGC | 7 cut(s) | 290, 356, 490, 1449, 1980, 2170, 2486 |
| BspANI | GGCC | 4 cut(s) | 288, 989, 1182, 2048 |
| BspCNI | CTCAG | 7 cut(s) | 25, 68, 1634, 1889, 2146, 2371, 2377 |
| BspHI | TCATGA | 2 cut(s) | 1885, 2254 |
| BspLI | GGNNCC | 6 cut(s) | 111, 288, 847, 932, 989, 2220 |
| BspMAI | CTGCAG | 1 cut(s) | 1948 |
| BspMI | ACCTGC | 2 cut(s) | 1073, 2338 |
| BspPI | GGATC | 2 cut(s) | 1363, 2503 |
| BspQI | GCTCTTC | 1 cut(s) | 1494 |
| BspT104I | TTCGAA | 2 cut(s) | 1076, 1367 |
| BspT107I | GGYRCC | 1 cut(s) | 930 |
| BsrDI | GCAATG | 3 cut(s) | 1175, 1444, 2272 |
| BsrFI | RCCGGY | 2 cut(s) | 389, 2354 |
| BsrGI | TGTACA | 2 cut(s) | 712, 1666 |
| BsrI | ACTGG | 5 cut(s) | 1160, 2049, 2222, 2402, 2480 |
| BssAI | RCCGGY | 2 cut(s) | 389, 2354 |
| BssECI | CCNNGG | 5 cut(s) | 106, 536, 863, 864, 1754 |
| BssNI | GRCGYC | 1 cut(s) | 2408 |
| BssSI | CACGAG | 1 cut(s) | 1991 |
| BssT1I | CCWWGG | 1 cut(s) | 1754 |
| Bst2BI | CACGAG | 1 cut(s) | 1991 |
| Bst2UI | CCWGG | 4 cut(s) | 107, 487, 865, 2202 |
| Bst4CI | ACNGT | 5 cut(s) | 335, 351, 370, 1218, 1236 |
| Bst6I | CTCTTC | 9 cut(s) | 47, 163, 172, 255, 738, 1464, 1494, 2000, 2238 |
| BstACI | GRCGYC | 1 cut(s) | 2408 |
| BstAUI | TGTACA | 2 cut(s) | 712, 1666 |
| BstBI | TTCGAA | 2 cut(s) | 1076, 1367 |
| BstC8I | GCNNGC | 7 cut(s) | 290, 356, 1148, 1976, 1980, 1984, 2247 |
| BstDEI | CTNAG | 9 cut(s) | 12, 55, 973, 1621, 1822, 1876, 2133, 2379, 2385 |
| BstEII | GGTNACC | 1 cut(s) | 2350 |
| BstF5I | GGATG | 5 cut(s) | 102, 466, 1249, 2276, 2530 |
| BstHHI | GCGC | 1 cut(s) | 1146 |
| BstMAI | GTCTC | 4 cut(s) | 15, 324, 2421, 2426 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 1156, 1165, 2032 |
| BstNI | CCWGG | 4 cut(s) | 107, 487, 865, 2202 |
| BstNSI | RCATGY | 2 cut(s) | 719, 2573 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 798 |
| BstPI | GGTNACC | 1 cut(s) | 2350 |
| BstSCI | CCNGG | 5 cut(s) | 105, 485, 535, 863, 2200 |
| BstSFI | CTRYAG | 4 cut(s) | 347, 1944, 2115, 2628 |
| BstSLI | GKGCMC | 4 cut(s) | 290, 356, 864, 991 |
| BstV1I | GCAGC | 3 cut(s) | 1522, 2184, 2340 |
| BstV2I | GAAGAC | 2 cut(s) | 52, 2154 |
| BstX2I | RGATCY | 4 cut(s) | 15, 1417, 1555, 2495 |
| BstYI | RGATCY | 4 cut(s) | 15, 1417, 1555, 2495 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1621 |
| BsuI | GTATCC | 2 cut(s) | 322, 2584 |
| BsuRI | GGCC | 4 cut(s) | 288, 989, 1182, 2048 |
| BtsCI | GGATG | 5 cut(s) | 102, 466, 1249, 2276, 2530 |
| BtsIMutI | CAGTG | 4 cut(s) | 356, 528, 1167, 2409 |
| BveI | ACCTGC | 2 cut(s) | 1073, 2338 |
| Cac8I | GCNNGC | 7 cut(s) | 290, 356, 1148, 1976, 1980, 1984, 2247 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1088 |
| CciI | TCATGA | 2 cut(s) | 1885, 2254 |
| CfoI | GCGC | 1 cut(s) | 1146 |
| Cfr10I | RCCGGY | 2 cut(s) | 389, 2354 |
| Cfr13I | GGNCC | 9 cut(s) | 110, 286, 287, 644, 846, 987, 988, 1331, 2219 |
| CseI | GACGC | 1 cut(s) | 1493 |
| CsiI | ACCWGGT | 1 cut(s) | 2200 |
| Csp6I | GTAC | 6 cut(s) | 604, 713, 1667, 1723, 1768, 2120 |
| CspAI | ACCGGT | 2 cut(s) | 389, 2354 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 720, 755 |
| CviQI | GTAC | 6 cut(s) | 604, 713, 1667, 1723, 1768, 2120 |
| DdeI | CTNAG | 9 cut(s) | 12, 55, 973, 1621, 1822, 1876, 2133, 2379, 2385 |
| DraI | TTTAAA | 1 cut(s) | 1962 |
| Eam1104I | CTCTTC | 9 cut(s) | 47, 163, 172, 255, 738, 1464, 1494, 2000, 2238 |
| EarI | CTCTTC | 9 cut(s) | 47, 163, 172, 255, 738, 1464, 1494, 2000, 2238 |
| Ecl136II | GAGCTC | 1 cut(s) | 1408 |
| Eco130I | CCWWGG | 1 cut(s) | 1754 |
| Eco24I | GRGCYC | 4 cut(s) | 290, 991, 1177, 1410 |
| Eco47I | GGWCC | 5 cut(s) | 110, 644, 846, 1331, 2219 |
| Eco53kI | GAGCTC | 1 cut(s) | 1408 |
| Eco57I | CTGAAG | 2 cut(s) | 1398, 2678 |
| Eco81I | CCTNAGG | 1 cut(s) | 1621 |
| Eco88I | CYCGRG | 1 cut(s) | 1270 |
| Eco91I | GGTNACC | 1 cut(s) | 2350 |
| EcoICRI | GAGCTC | 1 cut(s) | 1408 |
| EcoO109I | RGGNCCY | 2 cut(s) | 110, 987 |
| EcoO65I | GGTNACC | 1 cut(s) | 2350 |
| EcoRI | GAATTC | 5 cut(s) | 341, 2108, 2278, 2705, 2744 |
| EcoRII | CCWGG | 4 cut(s) | 105, 485, 863, 2200 |
| EcoT14I | CCWWGG | 1 cut(s) | 1754 |
| EcoT22I | ATGCAT | 1 cut(s) | 1402 |
| EcoT38I | GRGCYC | 4 cut(s) | 290, 991, 1177, 1410 |
| ErhI | CCWWGG | 1 cut(s) | 1754 |
| Esp3I | CGTCTC | 3 cut(s) | 15, 324, 2421 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1473, 1505, 2146, 2178 |
| FaqI | GGGAC | 2 cut(s) | 859, 2395 |
| FauI | CCCGC | 3 cut(s) | 297, 363, 1987 |
| FbaI | TGATCA | 2 cut(s) | 403, 1852 |
| Fnu4HI | GCNGC | 5 cut(s) | 1511, 2170, 2173, 2329, 2487 |
| FokI | GGATG | 5 cut(s) | 89, 473, 1256, 2283, 2537 |
| FriOI | GRGCYC | 4 cut(s) | 290, 991, 1177, 1410 |
| Fsp4HI | GCNGC | 5 cut(s) | 1511, 2170, 2173, 2329, 2487 |
| FspBI | CTAG | 4 cut(s) | 936, 1730, 1755, 2603 |
| FspI | TGCGCA | 1 cut(s) | 1145 |
| GlaI | GCGC | 1 cut(s) | 1145 |
| GluI | GCNGC | 5 cut(s) | 1511, 2170, 2173, 2329, 2487 |
| GsaI | CCCAGC | 1 cut(s) | 1346 |
| HaeIII | GGCC | 4 cut(s) | 288, 989, 1182, 2048 |
| HapII | CCGG | 3 cut(s) | 390, 536, 2355 |
| HgaI | GACGC | 1 cut(s) | 1493 |
| HhaI | GCGC | 1 cut(s) | 1146 |
| Hin1I | GRCGYC | 1 cut(s) | 2408 |
| Hin6I | GCGC | 1 cut(s) | 1144 |
| HinP1I | GCGC | 1 cut(s) | 1144 |
| HindIII | AAGCTT | 1 cut(s) | 1628 |
| HinfI | GANTC | 8 cut(s) | 239, 532, 727, 794, 1268, 1661, 1682, 2762 |
| HpaII | CCGG | 3 cut(s) | 390, 536, 2355 |
| HphI | GGTGA | 5 cut(s) | 404, 782, 2216, 2344, 2369 |
| Hpy166II | GTNNAC | 6 cut(s) | 526, 731, 893, 1549, 2086, 2360 |
| Hpy8I | GTNNAC | 6 cut(s) | 526, 731, 893, 1549, 2086, 2360 |
| Hpy99I | CGWCG | 2 cut(s) | 311, 374 |
| HpyCH4III | ACNGT | 5 cut(s) | 335, 351, 370, 1218, 1236 |
| HpyCH4IV | ACGT | 3 cut(s) | 309, 955, 2408 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 1156, 1165, 2032 |
| HpyF3I | CTNAG | 9 cut(s) | 12, 55, 973, 1621, 1822, 1876, 2133, 2379, 2385 |
| HpySE526I | ACGT | 3 cut(s) | 309, 955, 2408 |
| Hsp92I | GRCGYC | 1 cut(s) | 2408 |
| HspAI | GCGC | 1 cut(s) | 1144 |
| Ksp22I | TGATCA | 2 cut(s) | 403, 1852 |
| LguI | GCTCTTC | 1 cut(s) | 1494 |
| LmnI | GCTCC | 4 cut(s) | 1057, 1351, 1405, 1413 |
| Lsp1109I | GCAGC | 3 cut(s) | 1522, 2184, 2340 |
| LweI | GCATC | 6 cut(s) | 952, 1146, 1661, 1900, 2197, 2635 |
| MabI | ACCWGGT | 1 cut(s) | 2200 |
| MaeI | CTAG | 4 cut(s) | 936, 1730, 1755, 2603 |
| MaeII | ACGT | 3 cut(s) | 309, 955, 2408 |
| MaeIII | GTNAC | 6 cut(s) | 571, 1230, 1948, 2184, 2350, 2404 |
| MfeI | CAATTG | 1 cut(s) | 576 |
| MflI | RGATCY | 4 cut(s) | 15, 1417, 1555, 2495 |
| MhlI | GDGCHC | 6 cut(s) | 290, 356, 864, 991, 1177, 1410 |
| MlyI | GAGTC | 4 cut(s) | 233, 736, 788, 1262 |
| MmeI | TCCRAC | 4 cut(s) | 613, 704, 2578, 2601 |
| Mph1103I | ATGCAT | 1 cut(s) | 1402 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1391 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1038, 1286 |
| MspA1I | CMGCKG | 1 cut(s) | 2331 |
| MspI | CCGG | 3 cut(s) | 390, 536, 2355 |
| MspR9I | CCNGG | 5 cut(s) | 107, 487, 537, 865, 2202 |
| MunI | CAATTG | 1 cut(s) | 576 |
| Mva1269I | GAATGC | 1 cut(s) | 829 |
| MvaI | CCWGG | 4 cut(s) | 107, 487, 865, 2202 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 1156, 1165, 2032 |
| NciI | CCSGG | 1 cut(s) | 537 |
| NlaIV | GGNNCC | 6 cut(s) | 111, 288, 847, 932, 989, 2220 |
| NmuCI | GTSAC | 5 cut(s) | 571, 1230, 2184, 2350, 2404 |
| NsbI | TGCGCA | 1 cut(s) | 1145 |
| NsiI | ATGCAT | 1 cut(s) | 1402 |
| NspI | RCATGY | 2 cut(s) | 719, 2573 |
| NspV | TTCGAA | 2 cut(s) | 1076, 1367 |
| PagI | TCATGA | 2 cut(s) | 1885, 2254 |
| PasI | CCCWGGG | 1 cut(s) | 864 |
| PciSI | GCTCTTC | 1 cut(s) | 1494 |
| PctI | GAATGC | 1 cut(s) | 829 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1391 |
| PfeI | GAWTC | 4 cut(s) | 532, 1661, 1682, 2762 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 492 |
| PinAI | ACCGGT | 2 cut(s) | 389, 2354 |
| PkrI | GCNGC | 5 cut(s) | 1512, 2171, 2174, 2330, 2488 |
| PleI | GAGTC | 4 cut(s) | 233, 735, 788, 1262 |
| PpsI | GAGTC | 4 cut(s) | 233, 735, 788, 1262 |
| PpuMI | RGGWCCY | 1 cut(s) | 110 |
| PshAI | GACNNNNGTC | 1 cut(s) | 798 |
| PsiI | TTATAA | 1 cut(s) | 2520 |
| Psp124BI | GAGCTC | 1 cut(s) | 1410 |
| Psp5II | RGGWCCY | 1 cut(s) | 110 |
| Psp6I | CCWGG | 4 cut(s) | 105, 485, 863, 2200 |
| PspEI | GGTNACC | 1 cut(s) | 2350 |
| PspFI | CCCAGC | 1 cut(s) | 1342 |
| PspGI | CCWGG | 4 cut(s) | 105, 485, 863, 2200 |
| PspN4I | GGNNCC | 6 cut(s) | 111, 288, 847, 932, 989, 2220 |
| PspOMI | GGGCCC | 2 cut(s) | 286, 987 |
| PspPI | GGNCC | 9 cut(s) | 110, 286, 287, 644, 846, 987, 988, 1331, 2219 |
| PspPPI | RGGWCCY | 1 cut(s) | 110 |
| PstI | CTGCAG | 1 cut(s) | 1948 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1088 |
| PsuI | RGATCY | 4 cut(s) | 15, 1417, 1555, 2495 |
| PvuII | CAGCTG | 1 cut(s) | 2331 |
| RsaI | GTAC | 6 cut(s) | 605, 714, 1668, 1724, 1769, 2121 |
| RsaNI | GTAC | 6 cut(s) | 604, 713, 1667, 1723, 1768, 2120 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1038, 1286 |
| SacI | GAGCTC | 1 cut(s) | 1410 |
| SapI | GCTCTTC | 1 cut(s) | 1494 |
| SatI | GCNGC | 5 cut(s) | 1511, 2170, 2173, 2329, 2487 |
| Sau96I | GGNCC | 9 cut(s) | 110, 286, 287, 644, 846, 987, 988, 1331, 2219 |
| SchI | GAGTC | 4 cut(s) | 233, 736, 788, 1262 |
| ScrFI | CCNGG | 5 cut(s) | 107, 487, 537, 865, 2202 |
| SduI | GDGCHC | 6 cut(s) | 290, 356, 864, 991, 1177, 1410 |
| SexAI | ACCWGGT | 1 cut(s) | 2200 |
| SfaNI | GCATC | 6 cut(s) | 952, 1146, 1661, 1900, 2197, 2635 |
| SfcI | CTRYAG | 4 cut(s) | 347, 1944, 2115, 2628 |
| SfuI | TTCGAA | 2 cut(s) | 1076, 1367 |
| SgrAI | CRCCGGYG | 2 cut(s) | 389, 2354 |
| SinI | GGWCC | 5 cut(s) | 110, 644, 846, 1331, 2219 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1038, 1286 |
| SsiI | CCGC | 7 cut(s) | 290, 356, 490, 1449, 1980, 2170, 2486 |
| SspMI | CTAG | 4 cut(s) | 936, 1730, 1755, 2603 |
| SstI | GAGCTC | 1 cut(s) | 1410 |
| StyD4I | CCNGG | 5 cut(s) | 105, 485, 535, 863, 2200 |
| StyI | CCWWGG | 1 cut(s) | 1754 |
| TaaI | ACNGT | 5 cut(s) | 335, 351, 370, 1218, 1236 |
| TaiI | ACGT | 3 cut(s) | 312, 958, 2411 |
| TaqI | TCGA | 6 cut(s) | 440, 512, 545, 1076, 1238, 1367 |
| TatI | WGTACW | 4 cut(s) | 603, 712, 1666, 1722 |
| TauI | GCSGC | 2 cut(s) | 2172, 2489 |
| TfiI | GAWTC | 4 cut(s) | 532, 1661, 1682, 2762 |
| TscAI | CASTG | 4 cut(s) | 356, 528, 1167, 2409 |
| TseFI | GTSAC | 5 cut(s) | 571, 1230, 2184, 2350, 2404 |
| TseI | GCWGC | 3 cut(s) | 1510, 2172, 2328 |
| Tsp45I | GTSAC | 5 cut(s) | 571, 1230, 2184, 2350, 2404 |
| TspGWI | ACGGA | 4 cut(s) | 250, 259, 483, 1704 |
| TspRI | CASTG | 4 cut(s) | 356, 528, 1167, 2409 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 492 |
| VpaK11BI | GGWCC | 5 cut(s) | 110, 644, 846, 1331, 2219 |
| XceI | RCATGY | 2 cut(s) | 719, 2573 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1167 |
| XmaJI | CCTAGG | 1 cut(s) | 1754 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1391 |
| XspI | CTAG | 4 cut(s) | 936, 1730, 1755, 2603 |
| ZraI | GACGTC | 1 cut(s) | 2409 |
| Zsp2I | ATGCAT | 1 cut(s) | 1402 |